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38 results about "Staphylococcus carnosus" patented technology

Staphylococcus carnosus is a Gram-positive, coagulase-negative member of the bacterial genus Staphylococcus consisting of single and paired cocci. Its genome has the highest GC content - 36.4% - of any sequenced staphylococcal species.

Starter used for producing fermented bean curd and preparation method

The invention relates to a starter used for producing fermented bean curd and a preparation method, the starter is composed of bacterial strains of Micrococcus luteus, Kocuria kristinae, Staphylococcus carnosus, Leuconostoc mesenteroides, bacillus subtilis and Tetragenococcus halophilus, the above bacterial strains are subjected to high density culture, concentration, freeze drying protection andfreeze drying to obtain a freeze-dried fungus powder, the freeze-dried fungus powder is mixed with the above bacterial strains according to specific proportion of 3:2:1:1:2:1 to prepare the starter product which can be directly used for producing the fermented bean curd. The technology of the method is simple, the viable bacteria amount in the obtained starter is greater than 2.0*10<11> CFU / g freeze-drying powder. The starter has the advantages of high fermentation activity, less amount and good fermentation effect, thereby the fermentation period of the fermented bean curd is shortened by 60-70 days. The starter can be liquid. The method of the invention solves the problems of unstable product quality caused by competitor pollution and decreased production capacity of bacterial strain ina traditional natural fermentation process, extra protease addition is not required in the fermentation process, the fermentation period is effectively shortened, and the flavor of the product is unique.
Owner:黑龙江省克东腐乳有限公司

Method for producing bound trotters through artificial inoculation and fermentation

The invention discloses a method for producing bound trotters through artificial inoculation and fermentation, and belongs to the technical field of food processing. The method comprises the following steps: preparing trotter skin; pretreating raw materials; pickling the pretreated raw materials at a low temperature; rolling and kneading the pickled raw materials; inoculating and fermenting the kneaded raw materials; encrusting the fermented raw materials, and bundling up the encrusted raw materials; marinating the bundled raw materials; cooling the marinated raw materials, and vacuum packing the cooled raw materials; and sterilizing the packed raw materials at high pressure, cooling the sterilized raw materials, packing the cooled raw materials in boxes, and warehousing the boxes. According to the method disclosed by the invention, a conventional processing method is combined with a microorganism fermentation technique, mixed bacteria species of bulgaricus milk bacillus, saccharomyces cerevisia or bulgaricus milk bacillus and staphylococcus carnosus are used for fermentation in a bacteria species mixing manner, so that a novel and convenient ready-to-eat health food is provided for society. The bound trotters processed by the method disclosed by the invention are unique in special flavor, deep red in color, loose in meat quality, high in nutrient value, easy to preserve, stable in quality, and high in edible safety, and effectively reduce the content of nitrite.
Owner:XUZHOU UNIV OF TECH

Processing technology of meat products, and meat products obtained through processing technology

InactiveCN108208621AAvoid sour tasteGuaranteed consistency of tasteFood scienceFlavorStaphylococcus carnosus
The invention provides a processing technology of meat products, and meat products obtained through the processing technology. The processing technology of the meat products comprises the following steps: performing fermentation treatment on raw material meat through a fermenting agent so as to obtain fermented meat, wherein the fermenting agent comprises 60-75 wt.% of staphylococcus carnosus and25-40 wt.% of staphylococcus vitulinus; pickling the fermented meat to obtain pickled meat; and steaming the pickled meat so as to obtain the meat products disclosed by the invention. The fermenting agent consisting of the staphylococcus carnosus and the staphylococcus vitulinus is used for fermentation, two kinds of strains mutually cooperate, and the strains rapidly propagate through nutrients in the meat, so that the products produce unique fermented flavor; besides, through fermentation, the original color of the meat is displayed, and the color of the products is bright and similar with the original color of the meat; and besides, the fermenting agent does not generate acids, so that the problem that the taste of the meat products turns sour is solved. In addition, a steaming technology is combined, so that the usage of a conventional marinating and cooking technique is avoided, and the standardization of technology implementation and the taste consistency of the obtained meat products can be guaranteed.
Owner:内蒙古大牧场牧业(集团)有限责任公司 +1

Beef quality regulation and control method and beef product produced by same

The invention relates to a beef quality regulation and control method and a beef product produced by the method. The beef quality regulation and control method comprises the following steps: adding aleavening agent during pickling of a beef raw material, wherein the leavening agent comprises Staphylococcus carnosus and Staphylococcus vitulinus, pickling is performed for 24-72 hours at a temperature of 15-25 DEG C; and conducting drying, cooking and sterilization. According to the invention, the leavening agent is added during the pickling of the beef raw material, and drying, cooking and sterilization are then performed so as to achieve the quality regulation and control of the beef raw material, so the problems of dry and hard texture and color deterioration of the beef product can be improved, shearing force can be reduced by about 56%, and the product can be endowed with new flavor. The new micro-fermented beef product produced by the method disclosed by the invention is ruddy in color, easy to chew and unique in flavor, and has better market competitiveness.
Owner:CHINA MEAT RES CENT

Minced meat type fermented instant dried meat slices

The invention relates to minced meat type fermented instant dried meat slices. Fresh meat is used as a raw material and undergoes mincing, pickling, fermenting, sieving, baking, pressing and slicing to prepare instant dried meat slices; in the fermentation stage, a fermentation agent selected from THM-17 (staphylococcus xylosus and pediococcus pentosaceus), VHI-41 (staphylococcus xylosus, pediococcus pentosaceus and lactobacillus plantarum), PROMIX-5 (staphylococcus xylosus, lactobacillus paraplantarum and lactobacillus sake) and WBX-43 (staphylococcus xylosus and staphylococcus carnosus) is inoculated, wherein dried meat slices prepared from the fermentation agent WBX-43 and PROMIX-5 are good in texture, taste, color and overall acceptability, thus providing data support for actual development and production of the fermented dried meat slices and producing the minced meat type fermented instant dried meat slices good in taste and water binding capacity.
Owner:TIANJIN AGRICULTURE COLLEGE

Low-nitrite coarse cereal fermentation sausage and making method thereof

The invention discloses a low-nitrite coarse cereal fermentation sausage and a making method thereof, and belongs to the technical field of food processing. The making method provided by the inventioncomprises the following steps of adopting cow rhubarb sticks and chicken breasts as main materials, and coarse cereal powder, monascorubin and chitosan as auxiliary materials, adding bacterium lacticum, microzymes and staphylococcus carnosus separated and screened from sausages obtained by natural fermentation, and performing fermentation for 30-36h, so as to obtain the fermentation sausage. Theprepared low-nitrite coarse cereal fermentation sausage has the characteristics that the monascorubin and the chitosan are mixed according to the national standard to obtain a mixture, and the mixtureis used for replacing conventional nitrite for coloring, so that under the condition that the color is not influenced, the nitrite content in the sausage is reduced. In addition, coarse cereals are added for fermentation, so that the purpose that the coarse cereals and the meat products have complementary advantages in nutrients is realized. The content of dietary fibers in the sausage is increased, the sausage has the characteristics of being high in probiotic content and low in fat; and besides, mouth feel is improved, the palatability is improved, and the fermentation sausage has favorablemarket prospects.
Owner:JILIN UNIV

Staphylococcus carnosus capable of lowering biogenic amine and application of Staphylococcus carnosus

The invention discloses Staphylococcus carnosus capable of lowering biogenic amine and application of the Staphylococcus carnosus. The Staphylococcus carnosus is preserved in China General Microbiological Culture Collection Center which is located in Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing. The Staphylococcus carnosusis good in biogenic amine degradation ability and has an evident degradation effect on the biogenic amine such as tyramine, spermidine, putrescine and cadaverine which are common in fermented food, the tyramine degradation rate of the Staphylococcus carnosus is 50.88%, the spermidine degradation rate of the Staphylococcus carnosus is 51.83%, the putrescine degradation rate of the Staphylococcus carnosus is 31.14%, the cadaverine degradation rate of the Staphylococcus carnosus is 22.82%, and the Staphylococcus carnosus is stable in a soybean paste production environment and applicable to soybean paste fermentation.
Owner:JIANGNAN UNIV

Method of recycling kitchen waste effluent

The invention discloses a method of recycling kitchen waste effluent and relates to the technical field of resource recycling. The method includes the steps of adjusting pH of waste effluent to 6-8, adding soybean cake powder and molasses, heating to 80 DEG C and above to obtain a pretreated fermentation liquor; lowering the temperature of the pretreated fermentation liquor to 32 DEG C and below,adding a Bacillus siamensis agent and a Staphylococcus carnosus agent into the pretreated fermentation liquor, carrying out ventilated aerobic fermentation, and applying a fermentation product as a liquid microbial fertilizer to agricultural planting. The method has the advantages that the whole process is free of fouling odor, the liquid microbial fertilizer meets the national standard of microbial fertilizers, contains massive free amino acids and polypeptide ingredients, is more helpful for direct absorption by crops, can promote quick germination of seeds and development of roots, allows crops to mature early, and has a significant biocontrol function.
Owner:陕西天仁雪农业科技有限公司

Preparation method of low-mutton-smell fermented dried mutton slices

The invention discloses a preparation method of low-mutton-smell fermented dried mutton slices, which takes mutton as a raw material and comprises the following steps of: cleaning mutton, freezing, slicing and pickling; preparing a compound bacterium leavening agent consisting of lactobacillus plantarum, pediococcus pentosaceus and staphylococcus carnosus, adding the compound bacterium leavening agent into the pickled mutton slices, adding glucose as a carbon source, and fermenting; and drying and baking the fermented mutton slices to obtain the low-mutton-smell fermented dried mutton slices.The dried mutton slices prepared by the method are low in mutton smell, high in nutritional value, crispy in taste, rich in flavor, convenient to eat and suitable for various people to eat.
Owner:ZHEJIANG UNIV

Synthetic medium for Staphylococcus carnosus, and preparation method and application of Staphylococcus carnosus fermentation broth

The invention discloses a synthetic medium for Staphylococcus carnosus, and a preparation method and application of Staphylococcus carnosus fermentation broth. The synthetic medium for Staphylococcus carnosus comprises the following raw materials by weight: on the basis of a volume of 1 L, 10 to 50 g of anhydrous glucose, 0.1 to 1.5 g of lysine, 1.0 to 5.0 g of valine, 0.1 to 1.5 g of glycine, 1.0 to 5.0 g of arginine, 1.0 to 5.0 g of proline, 5 to 10.0 g of glutamic acid, 0.1 to 0.5 g of tryptophan, 0.1 to 1.5 g if cystine, 1.0 to 3.0 mg of thiamine hydrochloride, 1.0 to 3.0 mg of calcium pantothenate, 1.0 to 3.0 mg of nicotinic acid, 1.0 to 3.0 mg of manganese sulfate, 0.1 to 1.0 g of potassium dihydrogen phosphate, 0.1 to 0.8 g of magnesium sulfate heptahydrate, 0.005 to 0.010 g of ferrous sulfate heptahydrate, 1.0 to 5.0 g of dipotassium hydrogen phosphate trihydrate and 6 to 10 g of 3-(N-morpholino)propanesulfonic acid, with the balance being water. The invention also discloses a preparation method for the Staphylococcus aureus fermentation broth. The synthetic medium for Staphylococcus carnosus is reasonable in composition, rich in nutrients and capable of meeting the growth demands of Staphylococcus carnosus. The growth amount of Staphylococcus carnosus obtained through the synthetic medium provided by the invention is increased by 5% compared with the growth amount of Staphylococcus carnosus obtained through frequently used LB (Luria-Bertani) mediums.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY

Rapid gradient fermentation process for normal-temperature ready-to-eat leisure fermented meat and application of rapid gradient fermentation process

The invention provides a rapid gradient fermentation process for normal-temperature ready-to-eat leisure fermented meat and an application of the rapid gradient fermentation process. The normal-temperature ready-to-eat leisure fermented meat comprises the following strains in percentage by mass of 20-25% of lactobacillus sake, 20-25% of pediococcus acidilactici, 20-25% of staphylococcus xylosus, 15-25% of staphylococcus carnosus and 10-20% of staphylococcus vitulinus. Compared with the prior art, a design compound bacteria is used for fermentation, a three-stage rapid gradient fermentation production process is designed, the processing time of products is short, the acidity of the products better conforms to the mouth feel of Chinese people, the products are more moist, the mouth feel requirement of consumers is met, and in addition, the shelf life of the products is long.
Owner:ANHUI THREE SQUIRRELS ELECTRONICS BUSINESS +1

Starter used for producing fermented bean curd and preparation method

The invention relates to a starter used for producing fermented bean curd and a preparation method, the starter is composed of bacterial strains of Micrococcus luteus, Kocuria kristinae, Staphylococcus carnosus, Leuconostoc mesenteroides, bacillus subtilis and Tetragenococcus halophilus, the above bacterial strains are subjected to high density culture, concentration, freeze drying protection and freeze drying to obtain a freeze-dried fungus powder, the freeze-dried fungus powder is mixed with the above bacterial strains according to specific proportion of 3:2:1:1:2:1 to prepare the starter product which can be directly used for producing the fermented bean curd. The technology of the method is simple, the viable bacteria amount in the obtained starter is greater than 2.0*10<11> CFU / g freeze-drying powder. The starter has the advantages of high fermentation activity, less amount and good fermentation effect, thereby the fermentation period of the fermented bean curd is shortened by 60-70 days. The starter can be liquid. The method of the invention solves the problems of unstable product quality caused by competitor pollution and decreased production capacity of bacterial strain in a traditional natural fermentation process, extra protease addition is not required in the fermentation process, the fermentation period is effectively shortened, and the flavor of the product is unique.
Owner:黑龙江省克东腐乳有限公司

Fermented meat paste and preparation method thereof[v]

The invention discloses fermented meat paste and a preparation method thereof. The fermented meat paste comprises fermented meat and a flavoring agent, every 100 g of fermented meat contains 3% of white granulated sugar, 10% of thick broad-bean sauce, 2% of chili powder, 15% of sour and hot cowpeas, 10% of vegetable oil, 0.5% of monosodium glutamate, 0.5% of cumin powder, 0.06% of Chinese pricklyash powder, 0.1% of pepper powder, 0.08% of ginger powder, 50% of purified water and 5% of sweet potato starch. A composite leavening agent prepared from pediococcus pentosaceus 37x-15 and staphylococcus carnosus is added into meat for fermentation, and the meat is stir-fried after fermentation is completed. The meat paste disclosed by the invention is strong in sauce flavor, red and bright in color, moderate in sour and spicy taste and thick in paste body, and is a convenient table food with rich nutrition and unique flavor.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Method for electroporation of Staphylococcus carnosus and its application

The invention discloses a method for electroporation of Staphylococcus carnosus. The method comprises the following three steps: preparation of competent cells, electroporation, and resuscitation and culture. In the step of preparation of competent cells, Staphylococcus carnosus ATCC 51365 is activated in a LB medium and then inoculated into a growth medium B2, then undergoes shaking culture until OD578 is 0.6, and then is washed with a GS electric shock buffer. In the step of electroporation, to be transformed plasmids and the competent cells are uniformly mixed and subjected to ice-bath treatment and electroporation. In the step of resuscitation and culture, 1 mL of a LBM resuscitation medium is rapidly added into an electric shock cup after electric shock, the plasmids and the competent cells are taken out after uniform mixing and subjected to resuscitation and culture for 4 h, and the plasmids and the competent cells then coat a plate for culture overnight so as to obtain recombinant Staphylococcus carnosus with the introduced plasmids. With the method provided by the invention, conversion efficiency reaches 5.95 * 10<3> CFU / [mu]g DNA, so the conversion efficiency of Staphylococcus carnosus is greatly improved.
Owner:TIANJIN UNIV OF SCI & TECH

Method for preparing low-salt dry-soused scomberomorus niphonius based on low-temperature air drying-inoculation fermentation

The invention relates to the field of deep processing of aquatic products, and discloses a method for preparing low-salt dry-soused scomberomorus niphonius based on low-temperature air-drying inoculation fermentation. The method comprises the following steps: (1) pretreating raw materials; (2) sousing; (3) cleaning and draining; (4) carrying out low-temperature air drying; (5) carrying out microwave drying; and (6) carrying out vacuum packaging. According to the invention, lactobacillus plantarum and staphylococcus carnosus are used as mixed fermenting agents, fat hydrolysis of fish meat is promoted through rapid fermentation and sousing, and the content of flavor precursor substance free fatty acid is increased; and by adopting a low-temperature air-drying process, excessive oxidation offat in scomberomorus niphonius meat is remarkably inhibited, and the strength of aldehyde and ester flavor compounds is improved so as to improve the volatile flavor quality of scomberomorus niphoniusand reserve nutritional and functional components of the dry-soused scomberomorus niphonius to the greatest extent. The prepared low-salt dry-soused scomberomorus niphonius product is fresh and tender in meat quality, golden in color and luster and moderate in salty taste.
Owner:ZHONGKAI UNIV OF AGRI & ENG

Staphylococcus carnosus IEF1018 and application thereof

The invention discloses a staphylococcus carnosus and application of the staphylococcus carnosus in making sauce koji through solid state fermentation. The staphylococcus carnosus strain is separated from a sauce koji sample in a traditional solid state fermentation process and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.21620, and the preservation date is January 13, 2021. When the staphylococcus carnosus and aspergillus oryzae are mixed and fermented in a solid state to prepare the sauce koji, the inhibition effect on the growth of aspergillus oryzae hyphae is small, and it is observed through a scanning electron microscope that the staphylococcus carnosus can be directly adsorbed on the aspergillus oryzae hyphae, so that the staphylococcus carnosus can directly interact with the aspergillus oryzae. And when mixed koji-making is carried out for 48 hours, the protease activity of the sauce koji is improved by 40% than that of single koji-making of aspergillus oryzae, and a good industrial application performance is provided.
Owner:ZHEJIANG UNIV OF TECH

Poultry liver flavor sauce and processing method thereof

The invention provides a poultry liver flavor sauce and a processing method thereof. The processing method comprises the following steps of: dipping fresh poultry livers in black tea saline water andperforming chopping and stirring into liver paste, mixing soybean drawing protein, performing cooking, then pouring the cooked liver paste into a mixed extraction solution of fig, Nanguo pear and tremella fuciformis, conducting ultrasonic oscillation to obtain pretreated liver paste, then inoculating staphylococcus carnosus, saccharomyces cerevisiae and lactobacillus pentosus into the pretreated liver paste, and performing fermentation to obtain the poultry liver flavor sauce. Besides edible salt, other seasonings are not added, so that the product is natural and healthy, nutrient loss is effectively avoided in the processing process, the influence of fishy smell and bitter taste of poultry livers on the flavor of the product is eliminated, and a large number of protein molecules are degraded into small molecular peptides or amino acids which can be directly absorbed by the human body.
Owner:崔艳兰

Medicated diet chicken meal seasoning capable of tonifying Qi and blood and preparation method thereof

The invention discloses a medicated diet chicken meal seasoning capable of tonifying qi and blood and a preparation method thereof, and belongs to the technical field of food processing. The preparation method mainly comprises the steps that firstly, chicken is minced into chicken paste, lactococcus lactis, pediococcus pentosaceus, staphylococcus xylosus and staphylococcus carnosus are added to the chicken paste according to the volume ratio of 1:1:(1-6):(1-6), and after fermentation and enzymolysis, fermented chicken enzymatic hydrolysate is obtained; secondly, 0.1-0.3% of L-cysteine, 2-4% ofglucose and 3-7% of traditional Chinese medicine powder are added to the fermented chicken enzymatic hydrolysate, treatment is conducted for 60-80 minutes at the temperature of 75-85 DEG C, the pH value of 7-7.5 and the ultrasonic power of 200-300 W, and a crude chicken meal seasoning is obtained; thirdly, the crude chicken meal seasoning is subjected to filtration, preparation, suction filtration, sterilization and spray drying to obtain the chicken meal seasoning. The chicken meal seasoning prepared through the method is lasting in fermentation flavor, volatile substances are rich in type and high in content, the flavor level is obviously higher than the flavor level of the prior art, and the medicated diet chicken meal seasoning has the functions of promoting health and tonifying qi and blood.
Owner:NINGXIA UNIVERSITY

A Staphylococcus carnosus b1-2 with chromogenic effect and its application

The invention relates to staphylococcus camosus B1-2 with a chromogenic effect and application thereof, and belongs to the technical field of food fermentation. The strain B1-2 has classification nameStaphylococcus camosus, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 11, 2019; and the preservation number is CGMCC No.17325. The strain is appliedto preparation of a leavening agent to replace nitrite to realize a color protection effect. The staphylococcus camosus B1-2 with the chromogenic effect provided by the invention can replace the chromogenic effect of nitrite in meat products, and can be added into salami to realize nitrite-free fermentation.
Owner:BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY

Method for processing meat products by high-temperature fermentation technology

The invention provides a method for processing meat products by using a high-temperature fermentation technology, and belongs to the field of food processing. The method comprises the following stepsof pretreating raw material meat, mixing the pretreated raw material meat with ingredients, rolling and pickling to obtain pickled meat, fermenting the pickled meat by using a fermenting agent, a black bean peel extract and a cocoa extract to obtain fermented meat, and drying, cooking, cooling, packaging and sterilizing the fermented meat to obtain a fermented meat product. The fermenting agent comprises lactobacillus sake, pediococcus acidilactici, staphylococcus vitulinus and staphylococcus carnosus. The method for processing the meat product by utilizing the high-temperature fermentation technology has the advantages of short period and lower cost, and the prepared meat product is safe, good in flavor and high in nutritional value.
Owner:浙江波拉波拉食品股份有限公司 +1

A strain of Staphylococcus carnosus reducing biogenic amine and its application

The invention discloses a strain of Staphylococcus carnosus that reduces biogenic amines and its application. The Staphylococcus carnosus has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on July 25, 2019, and the preservation number is CGMCC No.18295 , and the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. The strain has a good ability to degrade biogenic amines, and has obvious degradation effects on tyramine, spermidine, putrescine and cadaverine, which are common biogenic amines in fermented foods, and the degradation rates are 50.88% and 51.83% respectively. , 31.14% and 22.82%, and has stability in the production environment of soybean paste, and can be used for fermentation of soybean paste.
Owner:JIANGNAN UNIV

Fermented earthworm powder and preparation method thereof

The invention provides fermented earthworm powder and a preparation method thereof. The preparation method comprises the following steps that earthworms are ground for 100-140 minutes, the grinding temperature is gradually increased to 78-80 DEG C from 60-62 DEG C, water is added in the grinding process, and earthworm grinding liquid is obtained after grinding is completed; a mixing agent is added into the earthworm grinding liquid and fermented at 78-82 DEG C, the mixing agent comprises, by mass, 66-70% of staphylococcus carnosus and 30-34% of neutral protease, and earthworm fermentation liquid is obtained after fermentation and enzymolysis; phosphate is added into the earthworm fermentation liquor to adjust the pH value to 4.5-6.5, and the stability of the earthworm fermentation liquor is maintained; and the earthworm fermentation liquor added with the phosphate is dried to prepare powder, thereby obtaining the fermented earthworm powder. Through proper grinding temperature and grinding time, protein is stably decomposed, release of active ingredients and beneficial ingredients of earthworms is promoted, and the broad-spectrum antibacterial and bacteriostatic performance can also be realized.
Owner:北京艾克赛德生物工程有限公司

Preservative for prolonging shelf life of pork and preparation method thereof

The invention discloses a preservative for prolonging the shelf life of pork and a preparation method thereof, and belongs to the technical field of pork freshness preservation. Raw materials comprise the following components in parts by weight: 15-25 parts of chitosan, 1-4 parts of lactic acid bacteria, 0.2-0.7 part of staphylococcus carnosus, 3-8 parts of soybean protein, 10-14 parts of cherry pit powder, and 3-5 parts of sorbitol. The preservative disclosed by the invention can be used for improving the saturation degree of pork intermuscular fat, so that the oxidation resistance, the pork color and the pH index of the pork are improved, and the shelf life of the pork is prolonged.
Owner:HEFEI UNIV OF TECH

Overexpressed UTP-glucose-1-phosphate-uridine transferase gene, and construction method and application of recombinant engineering bacterium of overexpressed UTP-glucose-1-phosphate-uridine transferase gene

The invention discloses an overexpression UTP-glucose-1-phosphate-uridine transferase gene, and a construction method and an application of a recombinant engineering bacterium of the overexpression UTP-glucose-1-phosphate-uridine transferase gene. The gene is a Lactobacillus acidophilus ATCC 4356 gene from a gene for encoding the UTP-glucose-1-phosphate-uridine transferase, and the nucleotide sequence of the overexpression UTP-glucose-1-phosphate-uridine transferase gene is represented by SEQ ID No:1 in a sequence table. The construction method comprises the following steps: the UTP-glucose-1-phosphate-uridine transferase gene segment of Lactobacillus acidophilus ATCC4356 is cloned and is connected to a pMG36e expression vector, the obtained substance is introduced to Staphylococcus carnosus or Lactococcus lactis by using an electric conversion method, and erythromycin resistance screening and identification are performed to obtain the recombinant engineering strain with the target gene, that is the engineering bacterium of the overexpressed UTP-glucose-1-phosphate-uridine transferase gene. The construction method has the advantages of significantly improving bacterial growth, enzyme activity and the freeze-drying survival rate.
Owner:NINGBO UNIV

Method for processing dry-type ripened beef tenderloin by using three-bacterium compound fermentation inoculant

PendingCN114794401AAccelerate the progress of maturationAdd flavorYeast food ingredientsPediococcusBiotechnologyStaphylococcus
The invention discloses a method for processing dry-type ripened beef tenderloin by using a three-bacterium compound fermentation inoculant, and belongs to the technical field of beef processing. The dry-type ripened beef tenderloin processing method using the three-bacterium compound fermentation inoculant comprises the following steps: cleaning and draining fresh beef tenderloin, and spraying a mixed bacterium suspension prepared by mixing staphylococcus carnosus, debaryomyces hansenii and pediococcus pentosaceus in proportion on the surface of the beef tenderloin; putting the beef tenderloin into a ripening cabinet for ripening to obtain the dry type ripening beef tenderloin; wherein the viable count ratio of the staphylococcus carnosus to the debaryomyces hansenii to the pediococcus pentosaceus in the mixed bacterial suspension is (1-2): (1-2): (1-2). The ripening time of the ripening beef tenderloin obtained by the processing method of the dry type ripening beef tenderloin is shortened to 14 days, the loss is reduced to 9.15%, the taste is soft, tender and juicy, the flavor is unique, and the cost is reduced.
Owner:JIANGNAN UNIV

Eriocheir sinensis fermented paste saucing base material and preparation method and application thereof

The invention belongs to the technical field of food processing, and provides an eriocheir sinensis fermented paste saucing base material and a preparation method and application thereof in order to solve the problem that current eriocheir sinensis marketing is restricted by the season, and consequently eriocheir sinensis cannot be eaten at any time. The eriocheir sinensis fermented paste saucingbase material is prepared in the mode that eriocheir sinensis high-pressure leaching liquor is subjected to mixed fermentation through staphylococcus xylosus, pediococcus pentosaceus, staphylococcus carnosus, THM-17 and WBX-43, wherein the inoculation amount of the staphylococcus xylosus, the pediococcus pentosaceus and the staphylococcus carnosus is 10<7> CFU / g, and the addition amount of the THM-17 and the WBX-43 is 0.2 g / kg. The cooked eriocheir sinensis high-pressure leaching liquor is fermented through the five fermentation agents commonly used for meat product processing, in order to facilitate transportation and storage, the group with the best flavor is selected to be thickened to make a paste to be applied into steamed buns with pork stuffing, thus the stuffing flavor has repeatability, and the reference value of the optimal adding amount of the fermented saucing base material is determined.
Owner:榭丰(江苏)农业科技有限公司

Processing method for enhancing chewiness of fish meat by using biological method

The invention discloses a processing method for enhancing chewiness of fish meat by using a biological method, which comprises the following steps: pretreating fish, draining water, smearing a composite biological fermentation agent which is 1-2% of the mass of the fish, and fermenting the fish at low temperature for 1.5-2.5 days, and taking out the fermented fish, wherein the compound biological leavening agent is prepared by mixing pediococcus pentosaceus, lactobacillus sake and staphylococcus carnosus according to the mass ratio of 1: 1: 3, and dissolving the mixture in water according to the mass ratio of 1:(10-20); and adding 2-3% of table salt, 0.03-0.05% of ginger powder and 0.03-0.05% of licorice powder based on the mass of the fish into the water. According to the processing method disclosed by the invention, the product is endowed with fragrant and sweet taste and chewy mouth feel, so that the single mouth feel and quality of the traditional frozen or hot-processed fish are changed; the invention also solves the problem of fishy smell of fishes, especially mud fishy smell of freshwater fishes; the product is chewy and elastic, has no bad taste, and is a nutritious food suitable for people of all ages.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Freeze-dried formulations of antibacterial protein

PendingUS20210161821A1Antibacterial agentsPowder deliveryBiotechnologyStaphylococcus pasteuri
A freeze-dried formulation includes an antibacterial protein having killing activity specific to at least one of or all following species: Staphylococcus arlettae, Staphylococcus aureus, Staphylococcus auricularis, Staphylococcus carnosus, Staphylococcus carprae, Staphylococcus chromogenes, Staphylococcus cohnii, Staphylococcus delphini, Staphylococcus epidermidis, Staphylococcus equorum, Staphylococcus gallinarum, Staphylococcus hemolyticus, Staphylococcus hominis, Staphylococcus intermedius, Staphylococcus kloosii, Staphylococcus lentus, Staphylococcus lugdunensis, Staphylococcus muscae, Staphylococcus pasteuri, Staphylococcus saprophyticus, Staphylococcus warneri, and Staphylococcus xylosus; a poloxamer; a sugar, and an amino acid.
Owner:INTRON BIOTECHNOLOGY INC

Combined microbial agent for fermenting food and application of combined microbial agent

PendingCN114107138ARealize Rational Construction StrategiesEasy to produceFungiBacteriaBiotechnologyMicrobial agent
The invention discloses a combined microbial agent for fermenting food and application of the combined microbial agent, and belongs to the technical field of microorganisms. According to the invention, the composite fermentation inoculant suitable for the broad bean mash of the Pixian bean paste is designed, and pediococcus acidilactici, staphylococcus carnosus and candida variabilis are sequentially inoculated in equal proportion, so that the quantity of flavor compounds generated by fermentation of the Pixian bean paste can reach 63.1% of that of in-situ fermentation, and the types of the flavor compounds are relatively similar; and the level of amino acid nitrogen reaches 0.45 g / 100g, which is far higher than 0.3 g / 100g specified by GB 2718-2014.
Owner:JIANGNAN UNIV +1
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