Combined microbial agent for fermenting food and application of combined microbial agent

A technology of combining bacterial agents and continuing fermentation, applied in the field of microorganisms, can solve the problems that microorganisms cannot adapt to high-salt environments, are easily infected with harmful substances, and are unstable, and achieve the effect of improving actual production.

Pending Publication Date: 2022-03-01
JIANGNAN UNIV +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Pixian Douban broad bean fermented grains are fermented by mixing koji with salt water. After mixing, most of the microorganisms contained in the koji cannot adapt to the high-salt environment
Therefore, during the fermentation process of Pixian Douban broad bean fermented grains, most of the microorganisms that directly affect th

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Combined microbial agent for fermenting food and application of combined microbial agent
  • Combined microbial agent for fermenting food and application of combined microbial agent
  • Combined microbial agent for fermenting food and application of combined microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The microbial screening of embodiment 1 combination microbial agent

[0026] First, the flavor components of the natural fermentation samples were used as evaluation criteria. Select target strains from complex communities using different screening conditions. Then, single-cell fermentations were performed in fully synthetic media to characterize the different strains. And carry out double-strain fermentation according to the function of bacterial strain. Based on the functional performance of single-strain and double-strain fermentations and microbial interactions, microbial communities with three or more microorganisms were established. Solid-state fermentation was carried out to evaluate the actual fermentation effect of the synthetic flora, and finally a combined bacterial agent inoculated with Pediococcus lactis, Staphylococcus carnosus, and Candida vulgaris in equal proportions was constructed. Specific steps include:

[0027] (1) Selection of target microorga...

Embodiment 2

[0052] Embodiment 2 uses three bacterium microorganism combination solid-state fermentation

[0053] In order to construct a synthetic microbiome capable of producing more flavor compounds and closer to the flavor composition of the in situ system, Lactococcus lactis-Staphylococcus carnosus-variable Candida is a three-bacteria combination inoculated in a specific order, and Pediococcus lactis, Staphylococcus carnosus, and Candida vulgaris are cultured to OD 600 =0.7, cultured to OD at a ratio of 2% 600 =0.7 Pediococcus lactis bacteria solution was inoculated into the synthetic medium, cultured for 48 hours and then inoculated and cultivated to OD in a proportion of 2% 600 =0.7 Staphylococcus carnus liquid, cultured for 48 hours and inoculated with 2% to OD 600 =0.7 of the Candida variabilis liquid, co-fermented for 6 days, the test results of volatile substances showed that, with the expansion of the scale of the synthetic microbiome, the number of flavors produced by the mi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a combined microbial agent for fermenting food and application of the combined microbial agent, and belongs to the technical field of microorganisms. According to the invention, the composite fermentation inoculant suitable for the broad bean mash of the Pixian bean paste is designed, and pediococcus acidilactici, staphylococcus carnosus and candida variabilis are sequentially inoculated in equal proportion, so that the quantity of flavor compounds generated by fermentation of the Pixian bean paste can reach 63.1% of that of in-situ fermentation, and the types of the flavor compounds are relatively similar; and the level of amino acid nitrogen reaches 0.45 g/100g, which is far higher than 0.3 g/100g specified by GB 2718-2014.

Description

technical field [0001] The invention relates to a combined microbial agent for fermented food and its application, belonging to the technical field of microbes. Background technique [0002] For thousands of years, fermented foods have been an important part of dinner tables around the world. Fermented food participates in the assembly of gut microbiome by providing vitamins, minerals, calories and other nutrients, and converts substrates into bioactive or bioavailable end products, some of which have antihypertensive, antihypertensive, Anti-cancer, anti-inflammatory, anti-diabetic, anti-thrombotic and anti-atherosclerotic functions. However, most fermented foods are open fermentation, and an important feature of this fermentation method is that it is uncontrollable. This leads to many problems, such as the intrusion of environmental microorganisms during the fermentation process leading to the production of harmful compounds. The introduction of artificially constructed ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N1/16A23L11/50A23L27/24C12R1/01C12R1/44C12R1/72
CPCC12N1/20C12N1/16A23L11/50A23L27/24A23V2002/00A23V2400/413A23V2250/76
Inventor 徐岩张丽杰郑鹏飞
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap