Combined microbial agent for fermenting food and application of combined microbial agent
A technology of combining bacterial agents and continuing fermentation, applied in the field of microorganisms, can solve the problems that microorganisms cannot adapt to high-salt environments, are easily infected with harmful substances, and are unstable, and achieve the effect of improving actual production.
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Embodiment 1
[0025] The microbial screening of embodiment 1 combination microbial agent
[0026] First, the flavor components of the natural fermentation samples were used as evaluation criteria. Select target strains from complex communities using different screening conditions. Then, single-cell fermentations were performed in fully synthetic media to characterize the different strains. And carry out double-strain fermentation according to the function of bacterial strain. Based on the functional performance of single-strain and double-strain fermentations and microbial interactions, microbial communities with three or more microorganisms were established. Solid-state fermentation was carried out to evaluate the actual fermentation effect of the synthetic flora, and finally a combined bacterial agent inoculated with Pediococcus lactis, Staphylococcus carnosus, and Candida vulgaris in equal proportions was constructed. Specific steps include:
[0027] (1) Selection of target microorga...
Embodiment 2
[0052] Embodiment 2 uses three bacterium microorganism combination solid-state fermentation
[0053] In order to construct a synthetic microbiome capable of producing more flavor compounds and closer to the flavor composition of the in situ system, Lactococcus lactis-Staphylococcus carnosus-variable Candida is a three-bacteria combination inoculated in a specific order, and Pediococcus lactis, Staphylococcus carnosus, and Candida vulgaris are cultured to OD 600 =0.7, cultured to OD at a ratio of 2% 600 =0.7 Pediococcus lactis bacteria solution was inoculated into the synthetic medium, cultured for 48 hours and then inoculated and cultivated to OD in a proportion of 2% 600 =0.7 Staphylococcus carnus liquid, cultured for 48 hours and inoculated with 2% to OD 600 =0.7 of the Candida variabilis liquid, co-fermented for 6 days, the test results of volatile substances showed that, with the expansion of the scale of the synthetic microbiome, the number of flavors produced by the mi...
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