Low-nitrite coarse cereal fermentation sausage and making method thereof
A low-nitrite, fermented sausage technology is applied in the field of low-nitrite miscellaneous grain fermented sausage and its production, which can solve the problems of high dietary fiber sausage quality and less research on taste, so as to solve the problem of poor taste and increase nutritional function. , the effect of increasing the content of beneficial bacteria
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Embodiment 1
[0028] A method for making fermented sausage with low nitrite miscellaneous grains, comprising the following steps:
[0029] Step 1: Screening of fermentation strains
[0030] (1) Take the sausage in the mid-stage of natural fermentation, cut it into pieces and homogenate;
[0031] (2) Dilute the homogenate to different concentrations with physiological saline and spread it on an MRS plate, incubate at 36±1°C for 48±1h, pick a single colony according to the color, size, gloss, transparency, etc. of the colony, and separate and purify by streaking;
[0032] (3) Screen dominant bacteria through tests such as salt tolerance test, acid production ability test, protein degradation activity, and fat degradation activity;
[0033] (4) Identify and screen strains according to the results of colony morphological characteristics, physiological and biochemical characteristics and 16SrRNA sequence analysis;
[0034] Step 2: Preparation of miscellaneous grain powder
[0035] (1) Stir-fr...
Embodiment 2
[0046] A method for making fermented sausage with low nitrite grains comprises the following steps:
[0047] Step 1: Screening of fermentation strains
[0048] (1) Take the sausage in the mid-stage of natural fermentation, cut it into pieces and homogenate;
[0049] (2) Dilute the homogenate to different concentrations with physiological saline and spread it on an MRS plate, incubate at 36±1°C for 48±1h, pick a single colony according to the color, size, gloss, transparency, etc. of the colony, and separate and purify by streaking;
[0050] (3) Screen dominant bacteria through tests such as salt tolerance test, acid production ability test, protein degradation activity, and fat degradation activity;
[0051] (4) Identify and screen strains according to the results of colony morphological characteristics, physiological and biochemical characteristics and 16SrRNA sequence analysis;
[0052]Step 2: Preparation of miscellaneous grain flour
[0053] (1) Stir-fry 17g each of oats...
Embodiment 3
[0064] Step 1: Screening of fermentation strains
[0065] (1) Take the sausage in the mid-stage of natural fermentation, cut it into pieces and homogenate;
[0066] (2) Dilute the homogenate to different concentrations with physiological saline and spread it on an MRS plate, incubate at 36±1°C for 48±1h, pick a single colony according to the color, size, gloss, transparency, etc. of the colony, and separate and purify by streaking;
[0067] (3) Screen dominant bacteria through tests such as salt tolerance test, acid production ability test, protein degradation activity, and fat degradation activity;
[0068] (4) Identify and screen strains according to the results of colony morphological characteristics, physiological and biochemical characteristics and 16SrRNA sequence analysis;
[0069] Step 2: Preparation of miscellaneous grain powder
[0070] (1) Stir-fry 15g each of oats, barley, buckwheat, amaranth seeds, and millet until the fragrance overflows, and grind into flour; ...
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