This invention relates to the fields of
molecular biology and
plant molecular marker breeding technology, and discloses an
Indel marker co-segregating with the
disease resistance trait in pepper and its application. The
Indel marker is located at 29,449,971-29,449,991 bp on
chromosome 5 of the pepper cv.CM334
genome; compared with the
disease-susceptible pepper, the
disease-resistant pepper exhibits a deletion of
Indel 73. The marker provided by this invention is tightly linked to the disease resistance
gene in pepper, exhibits stable localization, and is suitable for
molecular breeding; it allows for the detection of the disease resistance
genotype in pepper under laboratory conditions, without environmental limitations. Using this marker, rapid disease resistance testing of individual pepper plants can be performed at any growth stage without affecting their
normal growth; the
molecular marker of this invention can lay the foundation for
cloning the disease resistance
gene in pepper and studying the
molecular mechanism of disease resistance in pepper, and has application value in the breeding of new varieties.