10results about How to "Does not affect normal growth" patented technology

The invention provides an application of bakuchiol in preparation of a drug for resisting pseudomonas aeruginosa infection. A research result verifies that bakuchiol obviously inhibits gene expression related to a PQS quorum sensing system of the pseudomonas aeruginosa under the condition that normal growth of the pseudomonas aeruginosa is not hindered. Further analysis shows that the action mechanism of the bakuchiol definitely points to a transcriptional activator PqsR of a PQS quorum sensing system, in addition, the bakuchiol remarkably reduces the athletic ability of pseudomonas aeruginosa in an experiment, remarkably reduces gene expression related to a virulence factor, and has a significant effect in infection models of plants and animals. The bakuchiol shows the effect of remarkably weakening the toxicity of the pseudomonas aeruginosa on the Chinese cabbage and the caenorhabditis elegans.

The invention provides a culture medium for reducing the content of broussonetia papyrifera esterase and a tissue culture method, and belongs to the technical field of broussonetia papyrifera tissue culture. The culture medium for reducing the content of the broussonetia papyrifera esterase provided by the invention comprises an induction culture medium, a proliferation culture medium, a differentiation proliferation culture medium and a rooting culture medium, wherein the induction culture medium is composed of an MS culture medium, 6-BA and IAA; the proliferation culture medium is composed of a 1 / 2 MS culture medium, NAA, KT and IBA; the differentiation and proliferation culture medium consists of a B5 culture medium, folic acid, TDZ, 2, 4-D, sucrose and agar; the rooting culture medium consists of an N6 culture medium, gibberellin 3, ethylene, IAA, cane sugar and carrageenan. The culture medium and the tissue culture method provided by the invention can promote more than 98% of adventitious buds to root, ensure that the survival rate is more than 98% after acclimatization and transplantation, not only can effectively reduce the content of lignin in paper mulberry tissue culture seedlings, but also cannot influence the normal growth of the paper mulberry tissue culture seedlings.

The invention relates to the technical field of green prevention and control of plant diseases, in particular to application of a cucumber-marigold intercropping mode to prevention and control of cucumber root knot nematode diseases. Cucumbers and marigold are intercropped and cultivated, and cucumber root-knot nematode infection and / or reproduction are / is inhibited by the allelopathy of the marigold; wherein marigold plants are planted in soil and / or a culture medium, so that stable root systems are formed before cucumber planting; 7-21 days after field planting of the marigold, cucumber seedlings are planted in the same cultivation container or the adjacent rhizosphere space, the root system of the cucumber and the root system of the marigold are located in the same cultivation environment but not directly twined, and the marigold keeps surviving and growing in the early stage and the middle stage of cucumber growth. The method solves the problem that an existing intercropping mode lacks a system method with a cultivation structure and a planting time sequence as the core, and has the advantages that operation is easy and convenient, applicability is high, popularization is easy, compared with cucumber single cropping, the root knot index of cucumbers under an intercropping system is remarkably reduced, and the requirement for green agriculture development is met.

The invention belongs to the technical field of gene engineering, and discloses application of a rice gene OsUGT79 in regulation and control of rice seed germination under salt stress, the nucleotide sequence of the rice gene OsUGT79 is as shown in SEQ ID NO.1, and the sequence of protein coded by the rice gene OsUGT79 is as shown in SEQ ID NO.2. The method has the advantages that a new gene resource can be provided for molecular breeding and new germplasm creation related to plant salt-tolerant stress germination, and the method has a good application prospect and popularization value.

The invention relates to an insect-resistant agent and a prevention and control method for plant pests, and relates to the technical field of plant insect-resistant agents. Wherein the insect-resistant agent comprises a semen cuscutae extract; the preparation method of the semen cuscutae extract comprises the following steps: carrying out first extraction treatment on dried semen cuscutae powder by adopting a first solvent, and removing an extracting solution to obtain powder subjected to the first extraction treatment; the first solvent is an aliphatic alkane solvent; carrying out second extraction treatment on the powder subjected to the first extraction treatment by adopting a second solvent, and removing an extracting solution to obtain powder subjected to the second extraction treatment; the second solvent is an ester organic solvent; performing third extraction treatment on the powder subjected to the second extraction treatment by adopting a third solvent, and collecting a third solvent extracting solution; the third solvent is one or more of ethanol, methanol and water; and performing solvent removal treatment on the third solvent extracting solution to obtain the semen cuscutae extract. The invention mainly develops an insect-resistant agent which is green, efficient, low in toxicity and not easy to generate drug resistance.

A plurality of cultivation grooves are formed in a circulating fungus cultivation frame of the factory-like cultivation equipment, each cultivation groove is composed of an outer groove body and an inner groove body, a plurality of partition plates are arranged in the inner groove bodies and divide the inner groove bodies into a plurality of cultivation bins, long grooves are formed in the bottoms of the cultivation bins, and a plurality of sealing plates are connected to the bottoms of the outer groove bodies in a lifting mode. The closed plate is provided with a plurality of air inlets with air filters, the upper portion of each culture bin is further connected with a cover in an opening and closing mode, each cover is connected with a first connector, and one end of the outer groove body is further provided with a second connector. According to the ganoderma lucidum spore powder collecting device, spore powder sprayed out of ganoderma lucidum can be collected in time, and growth of other immature ganoderma lucidum cannot be affected.

The application discloses an application of a small-molecule compound in preventing and treating plant RNA virus infection and belongs to the technical field of biotechnology. 2'-amino-2'-deoxyguanosine (ADG) is applied as the only antiviral active ingredient in plant RNA virus prevention for the first time, only a low concentration of 250 muM is needed, and a simple exogenous spraying method can achieve the broad-spectrum inhibition of various plant RNA viruses such as turnip mosaic virus (TuMV), tomato mosaic virus (ToMV) and tobacco mosaic virus (TMV), and after spraying, the normal growth of plants is not affected, the risk of drug damage is completely avoided, the safety, high efficiency and broad spectrum of plant RNA virus prevention are significantly improved, and the application has important practical application significance for guaranteeing the yield and quality of crops in agricultural production and promoting the green transformation of agriculture.

The application belongs to the technical field of medical materials and relates to a bacteriostatic suture for surgical suturing and a preparation method thereof. The preparation method comprises the following steps: 2-amino-5-acetylpyridine is dissolved in a hydrobromic acid glacial acetic acid solution, an acetic acid solution containing bromine is added, and an intermediate 1 is obtained through reaction; the intermediate 1 is added to tetrahydrofuran, triethylamine and acetic anhydride are added, and an intermediate 2 is obtained through reaction; the intermediate 2 and thiourea are added to anhydrous ethanol, and an intermediate 3 is obtained through reaction; the intermediate 3 and p-nitrobenzaldehyde are added to anhydrous methanol, and an intermediate 4 is obtained through reaction; phosphorus oxychloride is added to N,N-dimethylformamide, and the intermediate 4 is added to obtain an intermediate 5 through reaction; the intermediate 5 is dissolved in a mixed solvent, and cystamine is added to obtain an antibacterial agent; and the medical silk suture is immersed in dimethyl sulfoxide containing the antibacterial agent, and is subjected to ultrasonic treatment, soaking, rinsing and drying to obtain the bacteriostatic suture. The bacteriostatic suture has the advantages of multi-mechanism synergistic enhanced bacteriostatic performance, an environment-responsive active release mechanism and excellent interface affinity.

The utility model discloses a facility vegetable plant hanging device which comprises two clamping pieces which are correspondingly matched, each clamping piece comprises a semicircular clamping part and a pressing part which is arranged in an inclined mode, and the pressing parts are fixedly connected with the end portions of the clamping parts through connecting parts. Fastening assemblies used for clamping plants are arranged in the two clamping parts, clamping holes which are correspondingly matched are formed in the opposite faces of the two connecting parts, clamping pieces are arranged in the clamping holes, the connecting positions of the two pressing parts are movably connected through a connecting shaft assembly, and spring pieces are installed on the opposite faces of the two pressing parts. After a plant is clamped, the fastening assembly is used for fastening the plant, so that the plant is fixed in the clamping part, and after the plant grows and becomes thick, the fastening assembly is extruded outwards, so that normal growth of the plant is not affected while the plant is kept clamped and fixed, and meanwhile, the situation that the lifting rope is loosened due to the fact that the device is opened is avoided.

This invention relates to the fields of molecular biology and plant molecular marker breeding technology, and discloses an Indel marker co-segregating with the disease resistance trait in pepper and its application. The Indel marker is located at 29,449,971-29,449,991 bp on chromosome 5 of the pepper cv.CM334 genome; compared with the disease-susceptible pepper, the disease-resistant pepper exhibits a deletion of Indel 73. The marker provided by this invention is tightly linked to the disease resistance gene in pepper, exhibits stable localization, and is suitable for molecular breeding; it allows for the detection of the disease resistance genotype in pepper under laboratory conditions, without environmental limitations. Using this marker, rapid disease resistance testing of individual pepper plants can be performed at any growth stage without affecting their normal growth; the molecular marker of this invention can lay the foundation for cloning the disease resistance gene in pepper and studying the molecular mechanism of disease resistance in pepper, and has application value in the breeding of new varieties.