The present application provides a mint
gene and a
cloning method and application thereof, wherein the
nucleotide sequence of the mint
gene is shown as SEQ ID NO. 1, and the MTPS1
gene is cloned from the mint in the following manner: the expressed sequences in leaves are assembled by using the
transcriptome sequencing of the mint; then, the PCR
specific primers are designed by using a primer
design software Primer Premier,
total RNA is extracted from the mint leaves, reverse transcription PCR is carried out, and the MTPS1 gene is cloned. When the MTPS1 is expressed in tomatoes, the synthesis of volatile aromatic substances in the fruits is changed, and it can be seen that the mint gene can change the volatile aromatic components of the
plant fruits.