Fermented oil with moisturizing, repairing and anti-oxidation effects, preparation method, application and daily chemical product

Abstract

The invention belongs to the technical field of cosmetic production, and discloses fermented oil with moisturizing, repairing and anti-oxidation effects, the fermented oil with the moisturizing, repairing and anti-oxidation effects is prepared by fermenting plant raw materials through strains, and the plant raw materials comprise soybeans, mint and onions. The strain is selected from at least one of lactobacillus plantarum, lactobacillus rhamnosus and pichia pastoris; the mass ratio of the soybeans to the mint to the onions in the plant raw materials is 3: (1-2): (0.1-1); the mass ratio of the strains to the plant raw materials is (1-3): 50; the fermented oil with good free radical scavenging capacity and cell migration promoting capacity is obtained by fermenting plant raw materials through the strain, and in addition, the invention further discloses a preparation method and application of the fermented oil with the moisturizing, repairing and anti-oxidation effects and cosmetics.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetic production, and in particular to a fermented oil with moisturizing, repairing and antioxidant effects, a preparation method, an application and cosmetics. Background Art

[0002] The skin barrier is the outermost layer of the skin, mainly composed of the stratum corneum and sebum membrane, and is an important protective device for the human body. The skin barrier has many key functions. It can block external physical damage, such as friction, scratches and pressure, and can also prevent harmful chemicals, pollutants and pathogens such as bacteria and viruses from invading the body. At the same time, the microbial community on the skin (skin microbiome) can inhibit the growth of harmful microorganisms and maintain skin health;

[0003] At the same time, the skin barrier maintains the skin's moisture content through lipids and natural moisturizing factors (such as urea, amino acids, lactic acid, etc.) in the stratum corneum, preventing the skin from becoming excessively dry and dehydrated.

[0004] The skin barrier is an important protective mechanism for maintaining skin and overall health. When the skin barrier is damaged (such as excessive cleansing, lack of moisturizing, environmental damage, etc.), it may lead to dry skin, redness, sensitivity, infection or other skin problems.

[0005] Aging and anti-aging are eternal topics. With the development of modern science, people have proposed many theories of aging, among which the theory of reactive oxygen free radicals has received more support. Normal physiological metabolism of the human body and environmental influences such as ultraviolet radiation and environmental pollution will lead to the generation of reactive oxygen (ROS). When the ROS content exceeds the body's ability to clear it, it breaks the balance between oxidation and anti-oxidation, causing oxidative stress. As the outermost tissue of the human body, the skin is directly exposed to the environment and is more likely to cause oxidative stress and oxidative damage. Oxidative damage caused by ROS includes damage to cell membranes, DNA, and proteins. In addition to causing skin aging, it can also cause skin tumors, photoaging, lupus erythematosus and other skin diseases. The antioxidant mechanism is roughly divided into scavenging free radicals, increasing the activity of antioxidant enzymes and reducing lipid metabolites, protecting important organelles of cells, regulating cell signal transduction pathways and inhibiting cell apoptosis at the cellular level. Since the antioxidant effect is often the result of the coordinated action of multiple mechanisms, cosmetics developed with different free radical scavengers or antioxidants can achieve the effect of delaying skin aging, and such cosmetics are also very popular among consumers.

[0006] Chinese patent application 201811612873.2 discloses a tyrosinase inhibitor and a whitening repair cream and a preparation method thereof. The tyrosinase inhibitor comprises a kudzu root extract and a mint extract. Based on the total mass of the tyrosinase inhibitor, the kudzu root extract is added in an amount of 0.1-55%, and the mint extract is added in an amount of 45-99.9%;

[0007] This solution effectively inhibits the activity of tyrosinase through the combination of kudzu root extract and peppermint extract, thereby reducing the production of melanin and achieving a whitening effect.

[0008] Chinese patent application 201910567504.4 discloses a cosmetic formula consisting of the following ingredients: 1-3 parts of dipropylene glycol, 2.5-3 parts of soy isoflavones, 0.5-1 parts of polyethylene glycol-40.5, 1-2 parts of butylene glycol, 0.1-50 parts of L-arginine, 0.8-1.2 parts of sodium hyaluronate, 2-5 parts of glycerol, 3-5 parts of oligopeptide-13, 0.5-1 parts of snow lotus extract, 2 parts of phenoxyethanol, 0.5-1 parts of tea extract, 0.8-1.5 parts of dipotassium glycyrrhizinate, 0.2-1 parts of trehalose, 0.3-1 parts of xanthan gum, 1-1.5 parts of hydroxyethyl cellulose, 0.1-30 parts of menthol, 0.5-0.8 parts of chlorphenesin, and the balance is pure water;

[0009] The cosmetics prepared by this scheme can be absorbed by skin massage, and L-citrulline is produced under the skin through endothelial nitric oxide synthase, and nitric oxide is released, which can increase capillary blood flow, promote collagen synthesis, improve skin quality, promote tissue regeneration, epithelial tissue healing, anti-inflammatory immunity and other functions. In addition, it can improve skin and mucous membrane damage, improve itching and allergies, and promote the healing of ulcers and ulcers.

[0010] The scheme explains the mechanism of action of its ability to improve skin and mucous membrane damage in paragraph 34 of the specification, specifically: "L-arginine and menthol added to cosmetics can produce L-citrulline and release nitric oxide under the skin through endothelial nitric oxide synthase. Nitric oxide can increase capillary blood flow under the skin, promote collagen synthesis, and improve skin quality. At the same time, nitric oxide has the functions of skin care, promoting tissue regeneration, epithelial tissue healing, anti-inflammatory immunity, etc. In addition, it contains rich physiologically active nutrients necessary for skin care, which can improve skin and mucous membrane damage, improve itching and allergies, and promote the healing of ulcers and ulcers."

[0011] The problem that this scheme needs to solve is: how to develop a fermented oil with good moisturizing, repairing and antioxidant abilities. Summary of the invention

[0012] The purpose of the present application is to provide a fermented oil with moisturizing, repairing and antioxidant effects, wherein the fermented oil is fermented by bacteria on multiple plant components to obtain a fermented product with good moisturizing, repairing and antioxidant effects;

[0013] Another object of the present application is to provide a method for preparing the fermented oil having moisturizing, repairing and antioxidant effects, as well as the use of the fermented oil having moisturizing, repairing and antioxidant effects, and a cosmetic.

[0014] To achieve the above-mentioned purpose, the present application discloses a fermented oil with moisturizing, repairing and antioxidant effects, which is obtained by fermenting plant raw materials with bacteria, wherein the plant raw materials are soybeans, mint and onions, and the bacteria are selected from at least one of Lactobacillus plantarum, Lactobacillus rhamnosus and Pichia pastoris;

[0015] The mass ratio of soybean, mint and onion in the plant raw material is 3:1-2:0.1-1;

[0016] The mass ratio of the bacterial strain to the plant raw material is 1 to 3:50.

[0017] Preferably, the mass ratio of soybean, mint and onion in the plant raw material is 3:1-1.5:0.1-0.3.

[0018] Soybeans contain natural fatty acids and proteins, which can moisturize and nourish the skin, helping to keep it soft and elastic; ground dry soybean powder can be used as a mild exfoliant to help remove dead skin cells on the skin surface, promote skin metabolism, and make the skin smoother; soybeans are rich in vitamins, minerals, and proteins, which can provide the skin with essential nutrients and help maintain skin health; soybeans also contain a large amount of antioxidants, which can fight free radicals, slow down skin aging, and reduce the appearance of fine lines and wrinkles; and the natural oils in soybeans can enhance the skin's barrier function, prevent moisture loss, and protect the skin from external environmental damage;

[0019] The menthol component in mint has a significant cooling sensation, which can quickly bring a soothing and refreshing feeling to the skin, and is particularly suitable for summer skin care products and after-sun repair products. Mint also has natural anti-inflammatory and calming effects, which can reduce skin redness and irritation, and is suitable for the care of sensitive skin and acne-prone skin. At the same time, mint has the function of regulating sebum secretion, which helps reduce the secretion of excess oil and keeps the skin refreshed, thereby preventing the formation of acne and blackheads. In addition, certain ingredients in mint can promote the repair and regeneration of skin cells, which helps accelerate wound healing and reduce scars.

[0020] Onions have natural antibacterial and anti-inflammatory properties that can help inhibit bacterial growth on the skin, reduce inflammation and infection, and are suitable for the care of acne and blackheads. In addition, onions are rich in antioxidants, such as vitamin C and polyphenols, which can neutralize free radicals, slow down the skin aging process, and protect the skin from environmental stress. At the same time, some ingredients in onion extracts can promote the metabolism and repair of skin cells, helping to accelerate skin wound healing and tissue regeneration.

[0021] Preferably, the bacterial strain is a mixed strain of Lactobacillus rhamnosus and Pichia pastoris, and the mass ratio of Lactobacillus rhamnosus to Pichia pastoris is 1 to 2:1.

[0022] Preferably, the fermentation product obtained by fermenting plant raw materials with bacteria is mixed with sodium hyaluronate;

[0023] The mass ratio of the fermentation product to sodium hyaluronate is 50:1.2-1.8.

[0024] Preferably, the sodium hyaluronate is full molecular weight sodium hyaluronate with an average molecular weight of 600±5 kDa.

[0025] In addition, the present application also discloses a method for preparing the above-mentioned fermented oil with moisturizing, repairing and antioxidant effects, comprising the following steps:

[0026] Step 1: Weigh and crush soybeans, mint and onion according to the mass ratio to obtain mixed powder;

[0027] Step 2: Mix the mixed powder with sodium dihydrogen phosphate, peptone and pure water to prepare a culture solution;

[0028] Step 3: Inoculate the bacteria into the culture medium and ferment for 20 to 26 hours, sterilize, and obtain the fermented oil with moisturizing, repairing and antioxidant effects.

[0029] Preferably, step 3 is specifically as follows: inoculating the bacteria into the culture solution and fermenting for 20 to 26 hours, sterilizing to obtain a fermentation product, and then mixing the fermentation product with sodium hyaluronate, homogenizing, and obtaining a fermented oil with moisturizing, repairing and antioxidant effects.

[0030] In addition, the present application also discloses the use of the fermented oil with moisturizing, repairing and antioxidant effects as described above in preparing cosmetics.

[0031] In addition, the present application also discloses a cosmetic containing 1 to 50 wt % of the fermented oil having moisturizing, repairing and antioxidant effects as described above.

[0032] More preferably, the cosmetic is in the form of a cream, lotion or spray.

[0033] The beneficial effects of the present application are as follows: the soybeans in the plant raw materials used in the present application contain natural fatty acids and proteins, which can moisturize and nourish the skin, and help keep the skin soft and elastic; the ground dry soybean powder can be used as a mild exfoliant, which helps to remove dead skin cells on the skin surface, promotes skin metabolism, and makes the skin smoother; soybeans are rich in vitamins, minerals, and proteins, which can provide essential nutrients for the skin and help maintain skin health; soybeans also contain a large amount of antioxidants, which can fight free radicals, slow down skin aging, and reduce the appearance of fine lines and wrinkles; and the natural oils in soybeans can enhance the skin's barrier function, prevent water loss, and protect the skin from external environment damage;

[0034] The menthol component in mint has a significant cooling sensation, which can quickly bring a soothing and refreshing feeling to the skin, and is particularly suitable for summer skin care products and after-sun repair products. Mint also has natural anti-inflammatory and calming effects, which can reduce skin redness and irritation, and is suitable for the care of sensitive skin and acne-prone skin. At the same time, mint has the function of regulating sebum secretion, which helps reduce the secretion of excess oil and keeps the skin refreshed, thereby preventing the formation of acne and blackheads. In addition, certain ingredients in mint can promote the repair and regeneration of skin cells, which helps accelerate wound healing and reduce scars.

[0035] Onions have natural antibacterial and anti-inflammatory properties, which can help inhibit bacterial growth on the skin, reduce inflammation and infection, and are suitable for the care of acne and blackheads. In addition, onions are rich in antioxidant ingredients, such as vitamin C and polyphenols, which can neutralize free radicals, slow down the skin aging process, and protect the skin from environmental stress. At the same time, some ingredients in onion extracts can promote the metabolism and repair of skin cells, helping to accelerate skin wound healing and tissue regeneration.

[0036] In addition, the present application further enhances the moisturizing, repairing and antioxidant capabilities of the fermented oil by fermenting the above-mentioned plant ingredients with bacteria and using the above-mentioned plant ingredients in combination. DETAILED DESCRIPTION

[0037] The present invention will be described clearly and completely below in conjunction with the embodiments of the present invention. In the description of the present invention, it should be noted that, if no specific conditions are specified in the embodiments, the conditions are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0038] Before presenting the examples, necessary descriptions are first made on the raw materials used in the examples. The supplier or source information of the raw materials in the examples is shown in Table 1:

[0039] Table 1

[0040]

[0041]

[0042] It should be noted that before the experiment, the bacteria were activated. The activation method was: the bacteria were inoculated onto an MRS plate culture medium and cultured at a constant temperature of 28° C. for 24 h to obtain a bacteria activation solution.

[0043] Furthermore, the method for preparing the fermented oil in the embodiment and the comparative example comprises the following steps:

[0044] Step 1: weigh soybeans, mint and onion according to the mass ratio, dry and crush to obtain mixed powder;

[0045] Step 2: Mix the mixed powder with sodium dihydrogen phosphate, peptone and pure water to prepare a culture solution, wherein the mass fraction of the mixed powder is 76%; the mass fraction of sodium dihydrogen phosphate is 1%; the mass fraction of peptone is 3%; and the mass fraction of pure water is 20%;

[0046] Step 3: Inoculate the strains into the culture medium in the form of strain activation liquid and ferment for 24 hours (the preparation methods of the strain activation liquid in the subsequent embodiments and comparative examples all adopt the above method, and the mass ratio of the mixed strains is the mass ratio of the corresponding strain activation liquid), then add lysozyme and neutral protease (the role of the above enzymes is only to sterilize the strains), sterilize and filter to obtain fermented oil with moisturizing, repairing and antioxidant effects;

[0047] Further, regarding the addition amount of each raw material in the mixed powder, the inoculation amount of the bacterial strain activation liquid and the specific selection of the bacterial strain in each embodiment and comparative example, refer to the specific formula of each embodiment and comparative example.

[0048] Examples 1-5

[0049] A fermented oil with moisturizing, repairing and antioxidant effects, the formula is shown in Table 2:

[0050] Table 2

[0051]

[0052] Example 6

[0053] The method is basically the same as Example 1, except that, when the total amount of bacteria added is the same, a mixture of Lactobacillus plantarum and Pichia pastoris is used to replace Lactobacillus rhamnosus, and the mass ratio of Lactobacillus plantarum to Pichia pastoris is 1.5:1.

[0054] Example 7

[0055] The method is basically the same as Example 1, except that, when the total amount of bacteria added is the same, a mixture of Lactobacillus rhamnosus and Pichia pastoris is used to replace Lactobacillus rhamnosus, and the mass ratio of Lactobacillus rhamnosus to Pichia pastoris is 1.5:1.

[0056] Example 8

[0057] The method is basically the same as Example 1, except that, when the total amount of bacteria added is the same, a mixture of Lactobacillus rhamnosus and Pichia pastoris is used to replace Lactobacillus rhamnosus, and the mass ratio of Lactobacillus rhamnosus to Pichia pastoris is 1:1.

[0058] Example 9

[0059] The method is basically the same as Example 1, except that, when the total amount of bacteria added is the same, a mixture of Lactobacillus rhamnosus and Pichia pastoris is used to replace Lactobacillus rhamnosus, and the mass ratio of Lactobacillus rhamnosus to Pichia pastoris is 2:1.

[0060] Example 10

[0061] The method is basically the same as Example 1, except that step 3 specifically comprises: inoculating the strain into the culture solution in the form of strain activation solution and fermenting for 24 hours, then adding lysozyme and neutral protease for sterilization and filtering to obtain a fermentation product;

[0062] The fermentation product is then mixed with sodium hyaluronate HA60 (with an average molecular weight of 600 kDa) and homogenized to obtain a fermentation oil with moisturizing, repairing and antioxidant effects.

[0063] Embodiment 11

[0064] The method is basically the same as Example 7, except that step 3 specifically comprises: inoculating the strain into the culture solution in the form of strain activation solution and fermenting for 24 hours, then adding lysozyme and neutral protease for sterilization and filtering to obtain a fermentation product;

[0065] The fermentation product is then mixed with sodium hyaluronate HA60 (with an average molecular weight of 600 kDa) and homogenized to obtain a fermentation oil with moisturizing, repairing and antioxidant effects.

[0066] Example 12

[0067] The method is basically the same as Example 1, except that step 3 specifically comprises: inoculating the strain into the culture solution in the form of strain activation solution and fermenting for 24 hours, then adding lysozyme and neutral protease for sterilization and filtering to obtain a fermentation product;

[0068] The fermentation product is then mixed with sodium hyaluronate HA60 (average molecular weight of 600 kDa and molecular weight distribution of 500-1000 kDa) and homogenized to obtain a fermentation oil with moisturizing, repairing and antioxidant effects.

[0069] Comparative Examples 1-5

[0070] A fermented oil, the formula is shown in Table 3:

[0071] Table 3

[0072]

[0073] Comparative Example 6

[0074] The method is substantially the same as Example 1, except that chickpeas of the same mass are used to replace soybeans.

[0075] Comparative Example 7

[0076] The method is substantially the same as Example 1, except that the mint is replaced with kudzu root of the same mass.

[0077] Comparative Example 8

[0078] The method is substantially the same as Example 1, except that honeysuckle of the same mass is used to replace onion.

[0079] Test Example 1

[0080] DPPH free radical scavenging activity assay

[0081] The fermented oil obtained in the embodiment and the comparative example was sampled, diluted to a concentration of 20% with pure water, and the DPPH free radical scavenging activity in the sample was measured. The specific method is as follows:

[0082] Accurately pipette 1.0mL of sample solution and mix it with the newly prepared 2.0mL DPPH working solution. Keep it in the dark at room temperature for 30 minutes and measure its absorbance B at 517nm. At the same time, measure the absorbance C of 2mL 95% ethanol solution and 1mL sample solution and the absorbance A of 2mL DPPH working solution and 1mL sample solvent. Take vitamin C as the positive control and water as the negative control. Repeat each sample and each concentration 3 times, take the average value, and calculate the DPPH free radical scavenging rate.

[0083] The DPPH free radical scavenging activity is shown in Table 4:

[0084] Table 4

[0085]

[0086] Result analysis:

[0087] It can be seen from Examples 1-5 that when the addition amount of the strain and the plant raw material is slightly changed, the DPPH radical scavenging rate of the fermented oil shows a certain fluctuation trend, but the overall amplitude is small. However, Example 1 has a more excellent DPPH radical scavenging rate than the other Examples 2-4. It can be seen that the addition amount of Example 1 is the best addition amount. On the other hand, it can be seen from Examples 3 and 5 that the DPPH radical scavenging rates of the two are relatively close. It can be seen that the fermented oil obtained by fermentation with Lactobacillus plantarum or Lactobacillus rhamnosus can produce good antioxidant effects.

[0088] By observing Example 1 and Examples 6-9, it can be seen that when Lactobacillus plantarum and Pichia pastoris are used for compounding, the DPPH radical scavenging rate of the fermented oil obtained in Example 6 is improved to a certain extent compared with Example 5, but the improvement is small, and when Lactobacillus rhamnosus and Pichia pastoris are used for compounding in Example 7, the DPPH radical scavenging rate of Example 7 is more significantly improved relative to Example 1; it can be seen that although the use of Pichia pastoris with Lactobacillus plantarum or Lactobacillus rhamnosus can improve the DPPH radical scavenging rate of the fermented oil, when Lactobacillus rhamnosus and Pichia pastoris are used for compounding, the DPPH radical scavenging rate improvement effect of Example 7 is more obvious, and this phenomenon may be caused by the fact that under this culture system, when Pichia pastoris and Lactobacillus rhamnosus are used together, the fermentation coordination ability between the two is stronger, thereby improving the content of substances with DPPH radical scavenging ability in the fermented oil;

[0089] Further observation of Examples 7-9 shows that when the mass ratio of Pichia pastoris to Lactobacillus rhamnosus is adjusted, the DPPH free radical scavenging rates of Examples 8 and 9 show a downward trend to varying degrees relative to Example 7. It can be seen that the mass ratio of Pichia pastoris to Lactobacillus rhamnosus in Example 7 is the optimal mass ratio; the reason for the decline may be that after adjusting the mass ratio of Pichia pastoris to Lactobacillus rhamnosus, the overall resource utilization capacity of the strain decreases, thereby resulting in a decrease in the content of substances with DPPH free radical scavenging ability;

[0090] It can be seen from Example 1 and Examples 10-12 that when the fermented product obtained by fermentation is further mixed with sodium hyaluronate to obtain fermented oil, the DPPH free radical scavenging rate of Example 10 is significantly improved compared with Example 1, and Example 11 is more significantly improved compared with Example 7; the reason for this phenomenon may be that sodium hyaluronate promotes the entry of substances with DPPH free radical scavenging ability into cells, and on the other hand, because the product obtained in Example 7 itself has good DPPH free radical scavenging ability, with the help of further penetration promotion of sodium hyaluronate, its free radical scavenging rate is further improved, and thus Example 11 is more significantly improved compared with Example 7;

[0091] It can be seen from Example 1 and Comparative Examples 1-3 that when any one of soybean, mint and onion is missing from the plant raw material, the free radical scavenging ability of Comparative Examples 1-3 shows a significant downward trend. It can be seen that soybean, mint and onion in the present application are indispensable.

[0092] Further observation of Comparative Examples 4-8 shows that when other bacterial species or other plant ingredients are used to replace the bacterial species and plant ingredients in the present application, the free radical scavenging abilities of Comparative Examples 4-8 show a significant downward trend. It can be seen that the bacterial species and plant raw materials in the present application are irreplaceable.

[0093] Test Example 2

[0094] Cell migration promotion efficacy assay

[0095] The fermented oils prepared in the examples and comparative examples were sampled, diluted to a concentration of 5% with pure water, and the cell migration promoting effects of the samples were measured. The specific method is as follows:

[0096] (1) Take 3T3 cells in the logarithmic growth phase and in good growth condition, digest them with trypsin to make a single cell suspension. Inoculate the cells in a 6-well plate, adjust the cell suspension to a cell concentration of 2E5 cells / mL, add 2 mL of complete culture medium to each well, and incubate the cells at 37°C and 5% CO2. When the cells are almost full, prepare to scratch, use a 200uL pipette tip to evenly scratch, then wash with 2mL / well PBS 2-3 times, wash away the suspended cells as much as possible, and after washing, replace the blank group with complete culture medium containing 1% serum, and the sample group contains the test substance (1%) and complete culture medium containing 1% serum. Observe the cell migration from 0 to 20 hours, take pictures and record, and then calculate the migration rate using ImageJ software.

[0097] The results of the cell migration promotion efficacy test in each case are shown in Table 5:

[0098] Table 5

[0099]

[0100]

[0101] Result analysis:

[0102] It can be seen from Examples 1-5 that when the addition amount of the strain and the plant raw material is slightly changed, the cell migration rate of the fermented oil shows a certain fluctuation trend, but the overall amplitude is small. However, Example 1 has a better cell migration rate than the other Examples 2-4. It can be seen that the addition amount of Example 1 is the best addition amount. On the other hand, it can be seen from Examples 3 and 5 that the cell migration rates of the two are relatively close. It can be seen that the fermented oil obtained by fermentation with Lactobacillus plantarum or Lactobacillus rhamnosus can produce good antioxidant effects.

[0103] By observing Example 1 and Examples 6-9, it can be seen that when Lactobacillus plantarum and Pichia pastoris are used for compounding, the cell migration rate of the fermented oil obtained in Example 6 is improved to a certain extent compared with Example 5, but the improvement is small, and when Lactobacillus rhamnosus and Pichia pastoris are used for compounding in Example 7, the cell migration rate of Example 7 is more significantly improved relative to Example 1; it can be seen that although the use of Pichia pastoris with Lactobacillus plantarum or Lactobacillus rhamnosus can improve the cell migration rate of the fermented oil, when Lactobacillus rhamnosus and Pichia pastoris are used for compounding, the cell migration rate improvement effect of Example 7 is more obvious, and this phenomenon may be caused by the fact that under this culture system, when Pichia pastoris and Lactobacillus rhamnosus are used together, the fermentation coordination ability between the two is stronger, thereby improving the content of substances that promote cell migration in the fermented oil;

[0104] Further observation of Examples 7-9 shows that when the mass ratio of Pichia pastoris to Lactobacillus rhamnosus is adjusted, the cell migration rates of Examples 8 and 9 show a downward trend to varying degrees relative to Example 7. It can be seen that the mass ratio of Pichia pastoris to Lactobacillus rhamnosus in Example 7 is the optimal mass ratio; the reason for the decline may be that after adjusting the mass ratio of Pichia pastoris to Lactobacillus rhamnosus, the overall resource utilization capacity of the strain decreases, thereby resulting in a decrease in the content of substances that promote cell migration ability;

[0105] It can be seen from Example 1 and Examples 10-12 that when the fermentation product obtained by fermentation is further mixed with sodium hyaluronate to obtain fermented oil, the cell migration rate of Example 10 is significantly improved compared with Example 1, and Example 11 is more significantly improved compared with Example 7; the reason for this phenomenon may be that sodium hyaluronate promotes the entry of substances that promote cell migration into cells, and on the other hand, because the product obtained in Example 7 itself has a good ability to promote cell migration, with the help of further penetration promotion of sodium hyaluronate, its cell migration rate is further improved, and thus Example 11 is more significantly improved compared with Example 7;

[0106] It can be seen from Example 1 and Comparative Examples 1-3 that when any one of soybean, mint and onion is missing from the plant raw material, the cell migration rates of Comparative Examples 1-3 all show a significant downward trend. It can be seen that soybean, mint and onion in the present application are indispensable.

[0107] Further observation of Comparative Examples 4-8 shows that when other bacterial species or other plant components are used to replace the bacterial species and plant components in the present application, the cell migration rates of Comparative Examples 4-8 all show a significant downward trend. It can be seen that the bacterial species and plant raw materials in the present application are irreplaceable.

[0108] Test Example 3

[0109] Stratum corneum moisture content test

[0110] 4.1 Test Method

[0111] 30 healthy male or female subjects aged 18 to 40 were selected to conduct the experiment in a constant temperature and humidity environment (20°C to 22°C, humidity 40% to 60%). Before the experiment, the subjects were required to sit quietly for 20 minutes in this environment. After the environment reached equilibrium, a random, single-blind, controlled test was performed. Blank controls (pure water) and test samples (the fermented oil obtained in Example 1-5 was sampled and diluted to a concentration of 5% with pure water) were used on the left and right forearms of the subjects according to the random principle. The concentration of (2.0±0.1) mg / cm 2 The tester will apply the dose to the test site before and 4 hours after the test. The CM825 skin moisture tester tested the skin moisture content of the experimental site. Each subject was measured 3 times and the average value was taken. The results are shown in Table 6:

[0112] Table 6

[0113]

[0114]

[0115] Compared with the blank control, after the products obtained in Examples 1-5 were applied to the skin, the skin moisture content was significantly increased, and the skin moisture content change rates of the remaining examples and comparative examples relative to the blank control group were all improved to a certain extent.

Claims

1. A fermented oil with moisturizing, repairing and antioxidant effects, characterized in that: The product is prepared by fermenting plant raw materials with bacteria, wherein the plant raw materials are soybeans, mint and onions, and the bacteria are selected from at least one of Lactobacillus plantarum, Lactobacillus rhamnosus and Pichia pastoris; The mass ratio of soybean, mint and onion in the plant raw material is 3:1-2:0.1-1; The mass ratio of the bacterial strain to the plant raw material is 1 to 3:

50.

2. The fermented oil with moisturizing, repairing and antioxidant effects according to claim 1, characterized in that: The mass ratio of soybean, mint and onion in the plant raw materials is 3:1-1.5:0.1-0.

3.

3. The fermented oil with moisturizing, repairing and antioxidant effects according to claim 1, characterized in that: The bacterial strain is a mixed bacterial strain of Lactobacillus rhamnosus and Pichia pastoris, and the mass ratio of Lactobacillus rhamnosus to Pichia pastoris is 1-2:

1.

4. The fermented oil with moisturizing, repairing and antioxidant effects according to claim 1, characterized in that: It is prepared by mixing the fermentation product obtained by fermenting plant raw materials with bacteria with sodium hyaluronate; The mass ratio of the fermentation product to sodium hyaluronate is 50:1.2-1.

8.

5. The fermented oil with moisturizing, repairing and antioxidant effects according to claim 4, characterized in that: The sodium hyaluronate is full molecular weight sodium hyaluronate with an average molecular weight of 600±5 kDa.

6. A method for preparing the fermented oil having moisturizing, repairing and antioxidant effects as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: Step 1: Weigh and crush soybeans, mint and onion according to the mass ratio to obtain mixed powder; Step 2: Mix the mixed powder with sodium dihydrogen phosphate, peptone and pure water to prepare a culture solution; Step 3: Inoculate the bacteria into the culture medium and ferment for 20 to 26 hours, sterilize, and obtain the fermented oil with moisturizing, repairing and antioxidant effects.

7. The method for preparing the fermented oil having moisturizing, repairing and antioxidant effects according to claim 6, characterized in that: The step 3 is specifically as follows: inoculating the bacteria into the culture solution and fermenting for 20 to 26 hours, sterilizing to obtain a fermentation product, and then mixing the fermentation product with sodium hyaluronate, homogenizing, and obtaining a fermented oil with moisturizing, repairing, and antioxidant effects.

8. Use of the fermented oil with moisturizing, repairing and antioxidant effects as claimed in any one of claims 1 to 5 in preparing cosmetics.

9. A cosmetic comprising 1 to 50 wt% of the fermented oil having moisturizing, repairing and antioxidant effects as claimed in any one of claims 1 to 5.

10. The cosmetic according to claim 9, characterized in that: The dosage form of the cosmetic is cream, lotion and spray.

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