15 results about "Turnip mosaic virus" patented technology

The invention discloses application of a small molecule compound in prevention and treatment of plant RNA virus infection, and belongs to the technical field of biology. According to the invention, 2 '-amino-2'-deoxyguanosine (ADG) is used as a unique antiviral active component to be applied to prevention and treatment of plant RNA viruses for the first time, and broad-spectrum inhibition of various plant RNA viruses such as turnip mosaic virus (TuMV), tomato mosaic virus (ToMV) and tobacco mosaic virus (TMV) can be realized only by a low concentration of 250 [mu] M and a simple exogenous spraying mode; and after spraying, the normal growth of plants is not influenced, the risk of phytotoxicity is thoroughly avoided, the safety, high efficiency and broad spectrum of plant RNA virus prevention and control are remarkably improved, and important practical application significance is achieved for guaranteeing the yield and quality of crops in agricultural production and promoting agricultural green transformation.

The invention discloses a plant endogenous small molecule polypeptide and application thereof in inhibiting RNA virus infected plants, the sequence of the small molecule polypeptide is Pep1-3: IIGVSNFDPVKRS, and the small molecule polypeptide is derived from nicotiana benthamiana; the invention relates to application of plant endogenous small molecule polypeptide in inhibiting RNA virus infection of plants. The plant is tobacco; the RNA viruses comprise tobacco mosaic viruses, tomato mosaic viruses and turnip mosaic viruses, and the small peptide Pep1-3 serves as a novel plant immunity inducer and shows the potential of the small peptide Pep1-3 in the aspect of preventing and controlling the RNA viruses. In agricultural production, prevention and treatment of virus diseases by adopting oligopeptide substances are a green and sustainable direction, and compared with traditional pesticides and plant immunity inducers, the composition has the advantages of broad spectrum, small toxicity, no drug resistance risk and long lasting period, and can generate a synergistic or synergistic effect when being mixed with other medicaments.

The present invention discloses the use of betulinic acid in the preparation of an anti-Tubulic Mosaic Virus preparation, relating to the technical field of agricultural plant protection. The present invention used 10 mg / L of betulinic acid to continuously root-irrigate Nicotiana benthamiana plants for 5 days, and found that betulinic acid treatment significantly inhibited TuMV infection and reduced viral accumulation levels compared to the control group. Betulinic acid, as a plant-derived substance, has its own degradation pathway and has the advantages of being safe, economical, and pollution-free, meeting the requirements of sustainable agricultural development and green environmental protection. The present invention makes it possible to use betulinic acid for the prevention and treatment of plant viruses, providing a new approach for the prevention and treatment of plant viruses.

The invention belongs to the technical field of plant molecular biology, and particularly relates to application of a Sec62 gene in plant antivirus and a cultivation method of an antiviral plant. A Sec62 overexpression plant can significantly inhibit system infection of BBSV, tobacco mosaic virus (TMV) and turnip mosaic virus (TuMV), so that the expression quantities of the three viruses in system leaves are significantly reduced. According to the invention, the antiviral function of the Sec62 gene is defined, broad-spectrum resistance to various viruses is realized, and based on plant endogenous gene regulation and control, the biological safety is high, the Sec62 gene can be used for antiviral breeding of crops, and a new strategy is provided for green prevention and control of plant virus diseases.

The present invention relates to the use of the MpAcrP5 protein in influencing the transmission of Turnip Mosaic Virus by Myzus persicae. The present invention discovers that the MpAcrP5 protein of Myzus persicae can influence the transmission of Turnip Mosaic Virus by Myzus persicae. Reducing the expression of the MpAcrP5 protein in Myzus persicae can reduce the transmission of Turnip Mosaic Virus by Myzus persicae. Specifically, dsRNA of the MpAcrP5 gene can be used to interfere with the transcription of the MpAcrP5 gene in Myzus persicae, thereby reducing the transmission of Turnip Mosaic Virus by Myzus persicae, thereby preventing or alleviating plant diseases caused by Turnip Mosaic Virus.

The invention belongs to the field of plant genetic engineering and synthetic biology, and particularly relates to a regulatory element TuMV-S modified by turnip mosaic virus 5 'UTR and application of the regulatory element TuMV-S in gene expression regulation. The nucleotide sequence of the regulatory element TuMV-S is as shown in SEQ ID NO. 2. Based on an agrobacterium tumefaciens-mediated tobacco instantaneous transformation system, through real-time fluorescent quantitative PCR, western blot and in-vitro enzyme activity experiments, it is found that compared with the situation that a viral element is not used, TuMV-S can improve the mRNA abundance of a target gene by about 8.4 times, increase the accumulation amount of zymoprotein by about 28.6 times and improve the catalytic activity of the zymoprotein by about 19.8 times. Therefore, the TuMV-S can significantly improve the mRNA stability and translation efficiency of the exogenous gene expressed in the tobacco cells, and effectively solves the problems of low expression intensity and instability of the current tobacco exogenous gene.

The application discloses an application of a small-molecule compound in preventing and treating plant RNA virus infection and belongs to the technical field of biotechnology. 2'-amino-2'-deoxyguanosine (ADG) is applied as the only antiviral active ingredient in plant RNA virus prevention for the first time, only a low concentration of 250 muM is needed, and a simple exogenous spraying method can achieve the broad-spectrum inhibition of various plant RNA viruses such as turnip mosaic virus (TuMV), tomato mosaic virus (ToMV) and tobacco mosaic virus (TMV), and after spraying, the normal growth of plants is not affected, the risk of drug damage is completely avoided, the safety, high efficiency and broad spectrum of plant RNA virus prevention are significantly improved, and the application has important practical application significance for guaranteeing the yield and quality of crops in agricultural production and promoting the green transformation of agriculture.

This invention discloses the application of the NbTuA gene in *Nicotiana benthamiana* in regulating plant resistance to turnip mosaic virus (CMV) and a method for cultivating transgenic plants. The NbTuA gene sequence is shown in SEQ ID NO:1. This invention involves cloning the NbTuA gene from *Nicotiana benthamiana* and constructing a vector fused with a YFP tag. Genetic transformation is then performed using Agrobacterium-mediated T-DNA transformation to construct transgenic plants of *Nicotiana benthamiana*. The *Nicotiana benthamiana* NbTuA transgenic plants obtained by this invention can significantly inhibit CMV infection.

The invention discloses a small peptide TaLEP1 and application thereof in antiviral defense of plants, and belongs to the technical field of agricultural biology. The amino acid sequence of the plant antiviral defense small peptide TaLEP1 is shown as SEQ ID NO: 1, and the small peptide TaLEP1 can be used for preparing plant antiviral products, including virus inhibitors and antiviral drugs, and is suitable for inhibiting wheat yellow mosaic virus, turnip mosaic virus and soybean mosaic virus of potyviridae Y. When in use, the TaLEP1 is dissolved in sterile PBS (Phosphate Buffer Solution) to prepare a 5 mu M working solution, the working solution is subjected to exogenous spraying on plant leaves, and after the plant leaves absorb the working solution, the TaLEP1 can induce activation of plant autophagy related pathways, so that virus infection is inhibited, and replication and accumulation of viruses in the plant bodies are reduced. The small peptide TaLEP1 provided by the invention can effectively inhibit the replication of various RNA viruses of potyviridae and has a good antiviral effect.

The invention relates to an application of an MpAcrP5 protein in influencing transmission of turnip mosaic virus by green peach aphid. The invention finds that the MpAcrP5 protein of green peach aphid can influence the transmission of turnip mosaic virus by green peach aphid, and when the expression quantity of the MpAcrP5 protein in green peach aphid body is reduced, the transmission of turnip mosaic virus by green peach aphid can be reduced. Specifically, dsRNA of the MpAcrP5 gene can be used for interfering transcription of the MpAcrP5 gene in the green peach aphid, so that turnip mosaic viruses transmitted by the green peach aphid are reduced, and the purpose of preventing or relieving plant diseases caused by the turnip mosaic viruses is achieved.

The invention belongs to the field of biological agriculture, and particularly relates to a polypeptide and application thereof. The amino acid sequence of the polypeptide is shown as SEQ ID NO: 1, and the polypeptide is derived from nicotiana benthamiana. The polypeptide is screened and identified from nicotiana benthamiana through a mass spectrum technology, and experiments prove that virus diseases caused by turnip mosaic virus, potato X virus, tomato mosaic virus and tobacco mosaic virus on tobacco are remarkably inhibited by applying the polypeptide. The polypeptide is derived from plants, has the advantages of being green, safe and not prone to generating drug resistance, and provides new bioactive molecules for green prevention and treatment of plant virus diseases.

The application discloses a small peptide TaLEP1 and application thereof in plant antiviral defense, and belongs to the field of agricultural biotechnology. An amino acid sequence of the plant antiviral defense small peptide TaLEP1 is shown as SEQ ID NO:1, and the small peptide TaLEP1 can be used for preparing plant antiviral products, including virus inhibitors and antiviral drugs, and is suitable for inhibiting wheat yellow mosaic virus, turnip mosaic virus and soybean mosaic virus of the Potyviridae family. When used, the TaLEP1 is dissolved in sterile PBS to prepare a 5 muM working solution, and is externally sprayed on plant leaves; after absorption of the plant, the TaLEP1 can induce the activation of a plant autophagy related pathway, and then inhibit virus infection and reduce the replication and accumulation of the virus in the plant. The small peptide TaLEP1 provided by the application can effectively inhibit the replication of various RNA viruses of the Potyviridae family, and has good antiviral effect.

The application belongs to the field of agricultural chemicals, and particularly relates to a compound with anti-potato virus Y virus activity and a preparation method and application thereof. 16 H 23 N5O7, the compound provided by the application has good inhibiting effect on viruses of the potato virus Y, such as potato virus Y, sugarcane mosaic virus and turnip mosaic virus, and can be used for preparation of anti-plant virus pesticides, and has wide application prospect.

The invention relates to the technical field of gene engineering, and particularly discloses an application of an MmFIT2 gene in plant broad-spectrum antivirus, which utilizes heterologous overexpression of the mouse gene MmFIT2 in nicotiana benthamiana to induce the increase of lipid droplets in cells, promote the increase of lipid droplet abundance, and improve the antiviral activity of plants. Normal growth and development of plants are not affected, and meanwhile the anti-virus capacity of the nicotiana benthamiana on barley stripe mosaic virus, tobacco mosaic virus, turnip mosaic virus and beet necrotic yellow vein virus is improved; by means of heterologous overexpression of the mouse-derived gene MmFIT2 in the rice, lipid droplets in cells are induced to be increased, lipid droplet abundance is promoted to be increased, normal growth and development of plants are not affected, and meanwhile the stripe virus resisting capacity of the rice is improved; the broad-spectrum applicability of a lipid droplet mediated antiviral strategy in nicotiana benthamiana and rice is proved, and an important value is provided for deeply exploring the application of lipid droplets in crop disease resistance breeding.

The invention discloses a TuMV CP as a target for screening medicines for preventing turnip mosaic viruses and application of the TuMV CP, by taking the TuMV CP as the target and integrating MST and ITC high-throughput screening, molecular docking, dynamic simulation, mutation verification and in-vivo experiments, the invention provides an efficient and specific medicine screening method, high-affinity compounds can be quickly identified, and the application of the TuMV CP in screening medicines for preventing turnip mosaic viruses is realized. The development period of medicines for preventing the turnip mosaic virus is shortened, and the prevention and treatment effect is improved. Compared with the prior art, the problems of poor target specificity, low screening efficiency and insufficient in-vivo verification are solved, the drug screening accuracy is improved, and the actual curative effect of the drug can be ensured.