35 results about "Turnip mosaic virus" patented technology

The invention claims a TuMV (Turnip Mosaic Virus) high-resistance RNA (Ribonucleic Acid) and an RNAi carrier for encoding the RNA. The TuMV high-resistance RNA consists of one of the following nucleotide sequences: 1) nucleotide sequence indicated by SEQ ID No.2 in a sequence table; 2) limited RNA sequence with more than 90% of homology and same functions, wherein concretely the homology is more than 95%, then concretely more than 96%, then concretely more than 97%, then concretely more than 98% and then concretely more than 99%. The RNA and the RNAi carrier for encoding the RNA are used for enhancing the resistance of the plant to TuMV or glufosinate-ammonium and meanwhile can be used for laying a foundation for cultivating the TuMV high-resistance transgenic plants.

The invention relates to a method for detecting a turnip mosaic virus (TuMV) by adopting a reverse transcription loop-mediated isothermal amplification (RT-LAMP) technology. The method comprises the following steps: performing total RNA extraction, RT-LAMP reaction, real-time turbidity detection and electrophoresis detection on the TuMV, and determining whether the TuMV is existent in a sample. RT-LAMP primers are designed according to a conservation region of the TuMV gene, and the method for quickly, sensitively and highly specifically detecting the TuMV is created by adopting the one-step RT-LAMP technology. A novel means is provided for quickly detecting the TuMV.

The invention discloses a hybridoma cell strain capable of secreting a tomato spotted wilt virus resistant monoclonal antibody and application of the monoclonal antibody. The preparation method comprises the following steps: immunizing a BALB / c mouse by taking tomato spotted wilt virus particles purified by a differential centrifugation method to serve as an antigen, performing cell fusion, screening and cloning, thereby obtaining a hybridoma cell strain 1A5 capable of performing stable passage and secreting the tomato spotted wilt virus resistant monoclonal antibody, wherein the collection number of the strain is CGMCC No.9343. The monoclonal antibody secreted by the hybridoma cells has the ascitic fluid ELISA titer of 10<-8>, and the type and sub-type of the antibody are IgG1 and kappa chain. A specific reaction is carried out between the monoclonal antibody secreted by 1A5 and the tomato spotted wilt virus only, and the monoclonal antibody does not react with turnip mosaic virus, tomato mosaic virus, iris yellow spot virus and cucumber mosaic virus; and moreover, the monoclonal antibody can be used for specifically and sensitively detecting the tomato spotted wilt virus. The hybridoma cell strain and the secreted monoclonal antibody provide technology and material supports for diagnosis, detection and scientific prevention and control of the tomato virus diseases.

The invention discloses a one-step multiplex reverse transcription-polymerase chain reaction (RT-PCR) method for detecting turnip mosaic virus (TuMV) and special primers for the method. The invention provides a primer group for detecting the turnip mosaic virus. The primer group is a primer group C, a primer group A or a primer group B shown in the specifications, wherein the primer group C consists of the primer group A and the primer group B; the primer group A comprises a primer pair a; the primer group B comprises a primer pair b; the primer pair a consists of a DNA molecule shown as a sequence 3 in a sequence table and a DNA molecule shown as a sequence 4 in the sequence table; and the primer pair b consists of a DNA molecule shown as a sequence 5 in the sequence table and a DNA molecule shown as a sequence 6 in the sequence table. Experiments prove that: the TuMV is quickly and accurately detected by the one-step multiplex RT-PCR method.

The invention provides a molecular marker closely linked with Chinese cabbage turnip mosaic virus resistance gene retrcs03 and an application thereof, belonging to the field of biotechnology. The molecular marker provided by the present invention further locates the Chinese cabbage TuMV resistance gene retrcs03, which helps to be better used in the Chinese cabbage resistance TuMV molecular marker-assisted selection, and at the same time, the gene can be located, and it can be used for the cloning and In-depth use and research to lay the foundation. At the same time, applying it to breeding work will greatly reduce the economic loss caused by the TuMV epidemic to Chinese cabbage production, which is beneficial to reduce production costs, and has great application potential and high economic value.

The invention discloses application of a TuMV-CP gene fragment-mediated RNAi carrier in cultivation of an anti-TuMV transgenic plant. The sequence 4 in a protection sequence table provided in the invention shows RNA fragments and its coding sequence. The RNA molecule can interfere with replication of TuMV RNA, thus inhibiting the TuMV. The invention also protects a transgenic plant cultivation method, which includes the following step of: expressing the RNA molecule in a starting plant so as to obtain a transgenic plant with higher TuMV resistance than that of the starting plant. The invention is of great value for cultivation of anti-TuMV plants (especially cruciferous plants).

The invention discloses cultivation and application methods of an arabidopsis nbr1 / atg8f double mutant. The cultivation method comprises determining that arabidopsis nbr1 and atg8f mutants can inhibitinfection turnip mosaic virus (TuMV), then crossing the arabidopsis nbr1 and atg8f mutants through genetic hybridization and screening offspring to obtain the arabidopsis nbr1 / atg8f double mutant. The obtained T3-generation arabidopsis nbr1 / atg8f double mutant plant can significantly inhibit infection of TuMV.