6 results about "Turnip mosaic virus" patented technology

The application discloses an application of a small-molecule compound in preventing and treating plant RNA virus infection and belongs to the technical field of biotechnology. 2'-amino-2'-deoxyguanosine (ADG) is applied as the only antiviral active ingredient in plant RNA virus prevention for the first time, only a low concentration of 250 muM is needed, and a simple exogenous spraying method can achieve the broad-spectrum inhibition of various plant RNA viruses such as turnip mosaic virus (TuMV), tomato mosaic virus (ToMV) and tobacco mosaic virus (TMV), and after spraying, the normal growth of plants is not affected, the risk of drug damage is completely avoided, the safety, high efficiency and broad spectrum of plant RNA virus prevention are significantly improved, and the application has important practical application significance for guaranteeing the yield and quality of crops in agricultural production and promoting the green transformation of agriculture.

This invention discloses the application of the NbTuA gene in *Nicotiana benthamiana* in regulating plant resistance to turnip mosaic virus (CMV) and a method for cultivating transgenic plants. The NbTuA gene sequence is shown in SEQ ID NO:1. This invention involves cloning the NbTuA gene from *Nicotiana benthamiana* and constructing a vector fused with a YFP tag. Genetic transformation is then performed using Agrobacterium-mediated T-DNA transformation to construct transgenic plants of *Nicotiana benthamiana*. The *Nicotiana benthamiana* NbTuA transgenic plants obtained by this invention can significantly inhibit CMV infection.

The invention belongs to the field of biological agriculture, and particularly relates to a polypeptide and application thereof. The amino acid sequence of the polypeptide is shown as SEQ ID NO: 1, and the polypeptide is derived from nicotiana benthamiana. The polypeptide is screened and identified from nicotiana benthamiana through a mass spectrum technology, and experiments prove that virus diseases caused by turnip mosaic virus, potato X virus, tomato mosaic virus and tobacco mosaic virus on tobacco are remarkably inhibited by applying the polypeptide. The polypeptide is derived from plants, has the advantages of being green, safe and not prone to generating drug resistance, and provides new bioactive molecules for green prevention and treatment of plant virus diseases.

The application discloses a small peptide TaLEP1 and application thereof in plant antiviral defense, and belongs to the field of agricultural biotechnology. An amino acid sequence of the plant antiviral defense small peptide TaLEP1 is shown as SEQ ID NO:1, and the small peptide TaLEP1 can be used for preparing plant antiviral products, including virus inhibitors and antiviral drugs, and is suitable for inhibiting wheat yellow mosaic virus, turnip mosaic virus and soybean mosaic virus of the Potyviridae family. When used, the TaLEP1 is dissolved in sterile PBS to prepare a 5 muM working solution, and is externally sprayed on plant leaves; after absorption of the plant, the TaLEP1 can induce the activation of a plant autophagy related pathway, and then inhibit virus infection and reduce the replication and accumulation of the virus in the plant. The small peptide TaLEP1 provided by the application can effectively inhibit the replication of various RNA viruses of the Potyviridae family, and has good antiviral effect.

The application belongs to the field of agricultural chemicals, and particularly relates to a compound with anti-potato virus Y virus activity and a preparation method and application thereof. 16 H 23 N5O7, the compound provided by the application has good inhibiting effect on viruses of the potato virus Y, such as potato virus Y, sugarcane mosaic virus and turnip mosaic virus, and can be used for preparation of anti-plant virus pesticides, and has wide application prospect.

The invention discloses a TuMV CP as a target for screening medicines for preventing turnip mosaic viruses and application of the TuMV CP, by taking the TuMV CP as the target and integrating MST and ITC high-throughput screening, molecular docking, dynamic simulation, mutation verification and in-vivo experiments, the invention provides an efficient and specific medicine screening method, high-affinity compounds can be quickly identified, and the application of the TuMV CP in screening medicines for preventing turnip mosaic viruses is realized. The development period of medicines for preventing the turnip mosaic virus is shortened, and the prevention and treatment effect is improved. Compared with the prior art, the problems of poor target specificity, low screening efficiency and insufficient in-vivo verification are solved, the drug screening accuracy is improved, and the actual curative effect of the drug can be ensured.