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Molecule labeling method of distinguishing mustard vegetables resisting turnip mosaic virus

A turnip mosaic virus, molecular technology, applied in the field of molecular biology, can solve problems that have not yet been

Inactive Publication Date: 2007-03-14
ZHEJIANG UNIV
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Problems solved by technology

So far, there are no molecular markers related to resistance to TuMV in mustard vegetables

Method used

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  • Molecule labeling method of distinguishing mustard vegetables resisting turnip mosaic virus

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Embodiment

[0026] Molecular Marker Method of Anti-TuMV in Brassica Vegetables

[0027] Mustard vegetables (Brassica juncea var.) were provided by our laboratory, and the materials were grown in Vegetable Research Institute, Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University.

[0028] (1) Primer design: According to the Brassica juncea anti-TuMV related gene BjRT in the Genebank database, (BjRT gene Genebank accession number: DQ789595, can be found in http: / / www.ncbi.nlm.nih.gov / ) design primers, Synthetic nucleotide molecules:

[0029] Forward primer: 5'-GCTGACCCAATAGTCTTGAT-3'

[0030] Reverse primer: 5'-TAGACGGAAATCCAAAGAG-3'

[0031] (2) Extraction of total plant DNA by CTAB method

[0032] (a) Take 0.5g of young leaves in a mortar, grind them under liquid nitrogen, add 600ul 2×CTAB preheated at 65°C (mixed with 30µl mercaptoethanol), transfer to a centrifuge tube, and keep warm at 65°C for 1-2 Hour.

[0033] (b) Add an equal volume of chlor...

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Abstract

The molecular marking process for identifying turnip mosaic virus (TuMV) resisting gene of mustard vegetables includes the following steps: extracting genome DNA's of different germplams of mustard vegetables, designing primer with the extracted genome DNA's as template according to the cloned TuMV resisting gene BjRT for PCR, agarose gel electrophoresis of the amplified product to detect the characteristic strip of TuMV resisting gene in mustard vegetables. The characteristic strip of TuMV resisting gene may be detected only in high TuMV resisting germplams. The present invention lays foundation for identifying TuMV resisting germplam resource of mustard vegetables.

Description

technical field [0001] The invention relates to a plant molecular marker technology, specifically a molecular marker method for identifying mustard vegetables resistant to turnip mosaic virus, and belongs to the technical field of molecular biology. Background technique [0002] With the development of molecular biology, molecular marker technology has become an important means of assisted breeding and identification of specific germplasm resources. The use of molecular marker assisted selection can be used for identification at the early stage of breeding such as the seedling stage; the selection process is not affected by factors such as the environment . Molecular markers commonly used in crop breeding include RFLP, RAPD, SSR and SCAP markers. The method adopts specific molecular marker technology to obtain molecular markers of mustard vegetable resistance to turnip mosaic virus (TuMV), and is used for screening and identification of specific germplasm resources of musta...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C07H21/04C12P19/34C12N15/11
Inventor 张明方杨景华许智颖
Owner ZHEJIANG UNIV
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