Molecule labeling method of distinguishing mustard vegetables resisting turnip mosaic virus
A turnip mosaic virus, molecular technology, applied in the field of molecular biology, can solve problems that have not yet been
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[0026] Molecular Marker Method of Anti-TuMV in Brassica Vegetables
[0027] Mustard vegetables (Brassica juncea var.) were provided by our laboratory, and the materials were grown in Vegetable Research Institute, Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University.
[0028] (1) Primer design: According to the Brassica juncea anti-TuMV related gene BjRT in the Genebank database, (BjRT gene Genebank accession number: DQ789595, can be found in http: / / www.ncbi.nlm.nih.gov / ) design primers, Synthetic nucleotide molecules:
[0029] Forward primer: 5'-GCTGACCCAATAGTCTTGAT-3'
[0030] Reverse primer: 5'-TAGACGGAAATCCAAAGAG-3'
[0031] (2) Extraction of total plant DNA by CTAB method
[0032] (a) Take 0.5g of young leaves in a mortar, grind them under liquid nitrogen, add 600ul 2×CTAB preheated at 65°C (mixed with 30µl mercaptoethanol), transfer to a centrifuge tube, and keep warm at 65°C for 1-2 Hour.
[0033] (b) Add an equal volume of chlor...
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