49 results about "Synthetic nucleotide" patented technology

Multimolecular devices and drug delivery systems prepared from synthetic heteropolymers, heteropolymeric discrete structures, multivalent heteropolymeric hybrid structures, aptameric multimolecular devices, multivalent imprints, tethered specific recognition devices, paired specific recognition devices, nonaptameric multimolecular devices and immobilized multimolecular structures are provided, including molecular adsorbents and multimolecular adherents, adhesives, transducers, switches, sensors and delivery systems. Methods for selecting single synthetic nucleotides, shape-specific probes and specifically attractive surfaces for use in these multimolecular devices are also provided. In addition, paired nucleotide-nonnucleotide mapping libraries for transposition of selected populations of selected nonoligonucleotide molecules into selected populations of replicatable nucleotide sequences are described.

Disclosed herein are nucleotide analogs with one or more protecting groups, methods of synthesizing nucleotide analogs with one or more protecting groups and methods of treating diseases and/or conditions such as viral infections, cancer, and/or parasitic diseases with the nucleotide analogs with one or more protecting groups.

The present invention is drawn to methods for designing synthetic nucleotide sequences encoding polypeptides of interest. The methods involve organizing a database of sequences as a set of N-length oligomer sequences and compiling a list of probability scores for each N-length sequence. The probability scores are used to substitute one or more higher-scoring sequences into the parent nucleotide sequence to generate an optimized sequence. The nucleotide sequence of interest may be further optimized by removing either or both of unintended open reading frames or undesired short DNA elements, and/or substituting oligomer sequences to achieve a specific G:C content. These methods may be used for optimizing expression of heterologous genes in any organism, particularly in plants. The method generates synthetic sequences with a composition similar to that of a target database. These synthetic sequences may be used, for example, for regulating pesticidal activity or herbicide resistance in organisms, particularly plants or plant cells.

The invention discloses a decoding and sequencing method by real-time synthesis of two nucleotides into deoxyribonucleic acid (DNA). Single-sequencing reactions are performed on two different nucleotides of X and Y simultaneously and a base sequence fragment code of XYn is obtained according to the quantitative relation between the number of synthesizing nucleotides and the number of the detected molecules which are generated in real time. The sequencing comprises two sets of sequencing reactions on the same template; and in either set of sequencing, a cycle that two different nucleotides synthesize the sequencing reaction simultaneously is performed on deoxyadenosine triphosphate (dATP), deoxycytidine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP) and deoxy-thymidine triphosphate (dTTP) containing four nucleotides in the mode that each nucleotide is used once in a cycle. After a plurality of sequencing reactions, information of a plurality of XYn ranked according to a sequencing order by the first set is obtained. When the set of sequencing reactions is completed, denaturation is performed to eliminate the extended strand of a sequencing primer and the sequencing primer is re-crossed to perform the second set of sequencing reactions; information of a plurality of XYn ranked by the second sequencing reaction is obtained; the specific base sequence of nucleic acid fragment to be detected is assembled by decoding the information of a plurality of XYn ranked according to a sequence order by the two sets.

The invention relates to a human papilloma virus 18 L1 (HPV18L1) polynucleotide sequence and its expression vector, host cell and use and especially relates to an amino acid sequence of an HPV L1 capsid protein, a synthetic nucleotide sequence for coding the amino acid sequence, a recombinant expression vector containing the synthetic nucleotide sequence, and a hansenula polymorpha expression host strain containing the synthetic nucleotide sequence. The invention also relates to a use of an HPV18L1 protein composed of the amino acid sequence and derivatives of the amino acid sequence in preparation of vaccines. Through modification of a nucleotide sequence of a gene of an HPV18L1 wide-type virus, a recombinant HPV18L1 capsid protein can be highly expressed in a hansenula polymorpha expression system and thus hansenula polymorpha expression system-based industrial production of an HPV18L1 capsid protein is realized. Compared with the existing eukaryotic expression systems, the HPV18L1 polynucleotide sequence and its expression vector and host cell have advantages of higher yield and lower cost.

The invention relates to an HPV16L1 polynucleotide sequence, and an expression vector, a host cell and application of the HPV16L1 polynucleotide sequence. The HPV16L1 polynucleotide sequence comprises an amino acid sequence of recombinant human papillomavirus (HPV) L1 capsid protein, a synthetic nucleotide sequence coding the amino acid sequence, and a recombinant expression vector and a hansenula polymorpha expression host strain comprising the nucleotide sequence. The invention also relates to the application of HPV16L1 protein consisting of the amino acid sequence and derivatives of the HPV16L1 polynucleotide sequence in preparing vaccine. According to the invention, by transforming the nucleotide sequence of HPV16L1 wild type virogene, the recombinant HPV16L1 capsid protein in a hansenula polymorpha system is efficiently expressed, and the HPV16L1 capsid protein can be industrially produced by using the hansenula polymorpha expression system; and compared with the conventional other eukaryotic expression systems, the hansenula polymorpha expression system has the advantages of high yield, low cost and the like.

A composition comprising: a plurality of identical first synthetic nucleotide oligomers; and a plurality of identical second synthetic nucleotide oligomers which are different to the first synthetic nucleotide oligomers, wherein each of the first synthetic nucleotide oligomers comprises a first primer binding sequence of bases, a first identifier sequence of three to seven bases in length, and a second primer binding sequence of bases, the first identifier sequence being disposed between the first and second primer binding sequences, wherein each of the second synthetic nucleotide oligomers comprises a third primer binding sequence of bases, a second identifier sequence of three to seven bases in length, and a fourth primer binding sequence of bases, the second identifier sequence being disposed between the third and fourth primer binding sequences, and wherein the first identifier sequence is different to the second identifier sequence.

According to the present invention, there is provided a fluorescent nucleotide represented by the formula: A-B-C,

wherein A represents a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue; B represents a divalent linking group or a single bond; and C represents a monovalent group derived from a fluorescent dye having 0 or 1 sulfonic acid group or phosphoric acid group in a molecule. The present invention provides useful fluorescent nucleotides for labeling nucleic acids, specifically, fluorescent nucleotides of which uptake ratio is high in synthetic reaction of nucleic acids.

The invention discloses an artificially synthesized nucleotide sequence, which is shown by SEQ ID NO: 1. The amino acid sequence for the disinsection protein of the nucleotide sequence coding is as shown by SEQ ID NO: 2. The invention also discloses a plasmid of the nucleotide sequence. The invention also disclose the use of the nucleotide sequence, which is a method of getting transgenic plant: the transgenic plant is stably guided. The invention can be used for detecting whether the nucleotide sequence is contained in the transgenic plant.

The invention discloses construction of a temperature-controlled aspergillus niger genetically engineered bacterium started to express an excision enzyme of cellulose and belongs to the field of enzyme engineering. The aspergillus niger genetically engineered bacterium disclosed by the invention is an aspergillus niger strain which is integrated with a gene of the excision enzyme of cellulose in a position of a heat shock protein gene. Construction of the aspergillus niger genetically engineered bacterium comprises the following steps: synthesizing a homologous recombinant fragment shown in a nucleotide sequence such as SEQ ID NO. 1; enzyme-digesting the recombinant fragment and a pWM1 plasmid by using Sa1I and then connecting transformed DH5alpha to obtain a homologous recombinant plasmid; transforming agrobacterium by using the homologous recombinant plasmid and obtaining the target strain by agrobacterium tumefaciens-mediated transformation. According to the invention, heat shock expression of the excision enzyme of cellulose is realized, so that the enzyme constitution of the cellulose of aspergillus niger is reasonable, so that the catalytic capacity of the cellulose is enhanced; the strain constructed does not generate toxins and is high in safety and suitable for industrial application.

The invention discloses a preparation method and application of antibacterial peptide Enterocin P. An antibacterial peptide Enterocin P gene has a nucleotide sequence shown as SEQ ID No.1. A mature antibacterial peptide Enterocin P coded by the antibacterial peptide Enterocin P gene has an amino acid sequence shown as SEQ ID No.2. The preparation method includes following steps: (1), artificially synthesizing a gene with a nucleotide sequence shown as SEQ ID NO:3; (2), building recombinant plasmid capable of realizing efficient expression; (3), building a gene engineering bacterium of lactococcus lactis NZ900; (4), utilizing the gene engineering bacterium to express the antibacterial peptide; (5), detecting antibacterial activity of the antibacterial peptide. The antibacterial peptide has high antibacterial activity to pathogenic bacteria and is safe, nontoxic and free of side effect.

Method for the synthesis of nucleotide derivatives wherein molecules of interest are grafted on the oligonucleotide with the help of a “click chemistry” reaction between an azide function on the molecule of interest and an alkyne function on the oligonucleotide, or between an alkyne function on the molecule of interest and an azide function on the oligonucleotide.

Intermediate molecules, notably alkyne functionalized oligonucleotides, grafted oligonucleotides, azide functionalized oligonucleotides, oligonucleotide micro arrays containing them and the use of those grafted oligonucleotides for biological investigation and for cell targeting.

The invention discloses a preparation method and application of a probiotic antibacterial peptide Enterocin B genetically engineered bacterium. A probiotic antibacterial peptide Enterocin B gene has a nucleotide sequence shown as SEQ ID No.1. Mature antibacterial peptide Enterocin B coded by the probiotic antibacterial peptide Enterocin B gene has an amino acid sequence shown as SEQ ID No.2. The preparation method includes following steps: (1), artificially synthesizing a gene with a nuceotide sequence shown as SEQ ID NO:3; (2), establishing recombinant plasmid supportive of efficient expression; (3), establishing a genetically engineered bacterium; (4), utilizing the genetically engineered bacterium to express the antibacterial peptide Enterocin B. The antibacterial peptide enterocin B has high bacteriostatic activity and is safet, nontoxic and free of side effect and antibiotic resistance.

The invention discloses amethod for constructing a cellulase high-yielding strain by expressing doubleactive protein of celluloseexonucleaseand endonuclease, and belongs to the field of enzyme engineering. According to the method, coding genes provided with doubleactive protein of celluloseexonucleaseand endonucleaseare integrated to a position of an aspergillusniger heat shock protein gene with adoption of homologous recombination and genetic engineering technologies. Specifically, a homologous recombinant fragment whose nucleotide sequence is represented by SEQ ID NO.1 is synthesized, is digested by Sal I and connected with pWM1 plasmid,DH 5 alpha is transformed, and homologous recombinant plasmid is obtained; the homologous recombinant plasmid is transformed to agrobacterium tumefaciens, and a cellulase high-yielding aspergillusniger strain is obtained through agrobacterium tumefaciens mediated transformation. Expression of interest protein is increased remarkably by increasing the temperature through heat shockexpression, so that the activities of the celluloseexonucleaseand endonuclease are improved remarkably, and the enzyme activity of celluloseis improved.

The invention belongs to the technical field of biology, in particular to an artificial codon-optimized pseudorabies virus gE protein as well as a coding gene and an application thereof. According tothe invention, the gE-like protein gene which can be recognized by a pseudorabies virus gE antibody is optimized and synthesized through an artificial codon, an expression vector is constructed by utilizing an artificially synthesized nucleotide sequence, and the specific recognition capability of the pseudorabies virus gE protein and the pseudorabies virus gE antibody is analyzed through westernblotting, ELISA and sequencing. The pseudorabies virus gE protein obtained by the invention can be used for preparing a differential diagnosis kit for porcine pseudorabies virus infected wild strains.

A biological system for generating and preserving a repository of personalized, humanized transplantable cells, tissues, and organs for transplantation, wherein the biological system is biologically active and metabolically active, the biological system having genetically reprogrammed cells, tissues, and organs in a non-human animal for transplantation into a human recipient, wherein the non-human animal does not present one or more surface glycan epitopes and specific sequences from the wild-type swine's SLA is replaced with a synthetic nucleotides based on a human captured reference sequence from a human recipient's HLA.

The invention discloses a DNA sequencing method using a nucleotide and a nucleotide with a reversibly blocked end 3'. X and Y* nucleotides simultaneously undergo a single sequencing reaction, whereinX represents a nucleotide with an unblocked end 3' and Y* is a nucleotide with a reversibly blocked end 3'. According to a quantitative relationship between the same number of detected molecules produced by two different nucleotides in the sequencing reaction and the number N of synthetic nucleotides, the sequencing information for a single sequencing reaction includes (N-1) specific bases X and 1or 0 coding XY. The whole sequencing process includes at least two sequencing reactions on the same template. Through comparing the two sets of sequencing information, the specific base sequence of the nucleic acid fragment to be tested is determined. Three groups of sequencing can also be performed. Through comparing the sequencing information obtained by the three sequencing reactions, the specific base sequences of the nucleic acid fragments to be tested are determined and the accuracy of sequencing is further improved.

The invention discloses a probiotic antimicrobial peptide Enterocin P, and a preparation method and application thereof. The probiotic antimicrobial peptide Enterocin P gene has nucleotide sequence disclosed as SEQ ID No.1. The mature antimicrobial peptide Enterocin P encoded by the probiotic antimicrobial peptide Enterocin P gene has amino acid sequence disclosed as SEQ ID No.2. The preparation method of the antimicrobial peptide Enterocin P comprises the following steps: (1) synthesizing gene of which the nucleotide sequence is disclosed as SEQ ID NO:3; (2) establishing a recombinant plasmid capable of expressing the antimicrobial peptide; (3) transforming the host bacterium by using the recombinant plasmid to establish a gene engineering bacterium; (4) expressing the antimicrobial peptide by using the gene engineering bacterium; and (5) detecting the antibacterial activity of the antimicrobial peptide. The probiotic antimicrobial peptide Enterocin P has antibacterial activity on pathogenic bacteria, and can be used as an animal feed additive.

The present invention relates to the identification of a nucleotide sequence that is conserved in the genome of the Mouse Hepatitis Virus (MHV) and to the design and construction of synthetic nucleotide primers and probes that target this conserved region. The invention further provides for methods and kits that use these primers and probes to detect the presence of Mouse Hepatitis virus in a test sample.

The invention relates to a method for synthesizing nucleotide pools in a high-throughput manner by the aid of semiconductor chips and assembling double-stranded DNA (deoxyribonucleic acid). The method includes steps of (1), dividing pre-synthetic double-stranded DNA into a plurality of DNA fragments; (2), designing nucleotide by the aid of the DNA fragments; (3), adding sequences to two ends of a primer which is the nucleotide, synthesizing nucleotide sequences and preparing the nucleotide sequences by the aid of the semiconductor chips; (4), carrying out first-round amplification by the aid of the primer with biotin labels; (5), recombining first-round PCR (polymerase chain reaction) products by the aid of at least one type of recombinant enzymes; (6), purifying PCR mixtures; (7), carrying out second-round PCR, and assembling nucleotide fragments to obtain assembled PCR products; (8), carrying out third-round PCR amplification on the PCR products by the aid of designed head and tail primers to obtain double-stranded DNA fragments. The method has the advantages of low cost and high one-step synthesis throughput.