50 results about "Synthetic nucleotide" patented technology

Method for the synthesis of nucleotide derivatives wherein molecules of interest are grafted on the oligonucleotide with the help of a “click chemistry” reaction between an azide function on the molecule of interest and an alkyne function on the oligonucleotide, or between an alkyne function on the molecule of interest and an azide function on the oligonucleotide. Intermediate molecules, notably alkyne functionalized oligonucleotides, grafted oligonucleotides, azide functionalized oligonucleotides, oligonucleotide micro arrays containing them and the use of those grafted oligonucleotides for biological investigation and for cell targeting.

Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions containing a synthetic nucleotide sequence encoding a Cryl Ac protein are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided, wherein the nucleotide sequences are set forth in SEQ ID NO:5, 1, 2, 3, or 4, as well as variants and fragments thereof.

A composition comprising: a plurality of identical first synthetic nucleotide oligomers; and a plurality of identical second synthetic nucleotide oligomers which are different to the first synthetic nucleotide oligomers, wherein each of the first synthetic nucleotide oligomers comprises a first primer binding sequence of bases, a first identifier sequence of three to seven bases in length, and a second primer binding sequence of bases, the first identifier sequence being disposed between the first and second primer binding sequences, wherein each of the second synthetic nucleotide oligomers comprises a third primer binding sequence of bases, a second identifier sequence of three to seven bases in length, and a fourth primer binding sequence of bases, the second identifier sequence being disposed between the third and fourth primer binding sequences, and wherein the first identifier sequence is different to the second identifier sequence.

A decoding and sequencing method by real-time synthesis of two nucleotides into DNA. Single-sequencing reactions are performed on two different nucleotides, namely, X and Y simultaneously, and a base sequence fragment code XYn is obtained according to the quantitative relation between the number of synthesizing nucleotides and the number of the detected molecules which are generated in real time. The entire sequencing comprises performing two sets of sequencing reactions on the same template, wherein each set of sequencing comprises four nucleotides, namely, dATP, dCTP, dGTP, and dTTP, and cycles of sequencing reactions by simultaneous synthesis of two different nucleotides are performed in a manner that each nucleotide is used only once in each cycle. After several sequencing reactions, a set of XYn information ranked in a sequencing order is obtained. When said set of sequencing reactions is completed, denaturation is performed to eliminate an extended strand of a sequencing primer and the sequencing primer is re-crossed to perform a second set of sequencing reactions, and then several XYn information ranked by the second sequencing reaction is obtained; and finally, a specific base sequence of a nucleic acid fragment to be detected is assembled by decoding two sets of XYn information ranked in a sequencing order.