73 results about "Chemo enzymatic" patented technology

This invention provides a direct method for monitoring bacterial transglycosylase activity using labeled substrates produced by chemo-enzymatic synthesis wherein the labels are selected to permit the detection of both polymeric and non-polymeric products simultaneously, either directly or following the separation of product from starting material. The invention promotes the discovery of new antibiotics with activity against bacterial transglycosylases by a) laying the groundwork for structural analysis of purified, active transglycosylase (which permits structure-based design); and b) providing an assay that can be used to screen for inhibitors.

In vitro/cell-free process of preparing a sialylated oligosaccharides are described. The sialylated oligosaccharides include gangliosides. The oligosaccharides linked to various moieties including sphingoids and ceramides. Novel compounds that comprise sphingoid groups are disclosed. The compounds include sialylated oligosaccharides including gangliosides as well as various sphingoids and ceramides.

The invention discloses a method for extracting isoflavone from kudzuvine root efficiently, and belongs to the technical field of the processing of agricultural products and food. The method comprises the following steps of: (1) crushing, namely crushing kudzuvine root to form 60 to 80-mesh kudzuvine root powder; (2) performing enzymolysis, namely adding 70 percent of ethanol into the kudzuvine root powder, adding cellulase and protease and performing the enzymolysis to obtain kudzuvine root extract, wherein 1 to 2 grams of the cellulase and the protease are added into each 100 milliliters of solution; and (3) extracting, namely performing ultrasonic-microwave processing on the obtained kudzuvine root extract from the step (2), concentrating under reduced pressure, freezing and drying to obtain kudzuvine root isoflavone. In the method, the kudzuvine root isoflavone is extracted by a compound enzymolysis technology of the cellulase and the protease and combining an ultrasonic-microwave assisted extraction technology, so the technical advantages of chemical enzymolysis and physical extraction are expressed fully, the cost is reduced, and the dissolution rate and extraction rate of the kudzuvine root isoflavone are improved, so that the extraction purity is greatly higher than that by conventional extraction method.

The invention relates to methods and compositions for causing the selective targeting and killing of tumor cells. The present invention describes prophylactic or therapeutic cancer vaccines based on purified TAA proteins or TAA-derived synthetic peptides altered by chemical, enzymatic or chemo-enzymatic methods to introduce αGal epitopes or αGal glycomimetic epitopes, in order to allow for enhanced opsonization of the antigen by natural anti-αGal antibodies to stimulate TAA capture and presentation, thereby inducing a humoral and cellular immune response to the TAA expressed by a tumor. The animal's immune system thus is stimulated to produce tumor specific cytotoxic cells and antibodies which will attack and kill tumor cells present in the animal.

The invention discloses a kinetic resolution method, in particular a method for preparing chemical enzyme of (R)-1-(1-naphthyl) ethylamine by resolution, which using 1-(1-naphthyl) ethylamine as a raw material. In the method, kinetic resolution is performed by using 1-(1-naphthyl) ethylamine which is a racemic mixture as a raw material, phenylethyl acetate as an acyl donor and immobilized lipase from Candida Antarctica as a catalyst under the controlled operation conditions that: the temperature is 30 to 80 DEG C, the volume ratio of the raw material to a solvent is 1:(25-50), the mass ratio of the raw material to the catalyst is 1:(0.02-0.05), and the molar ratio of the raw material to the phenylethyl acetate is 1:(1.2-4.0). The method has the advantages of high catalytic efficiency and environment friendliness.

A new class of paromomycin-derived aminoglycosides, which exhibit efficient stop-codon mutation suppression activity, low toxicity and high selectivity towards eukaryotic cells are provided. Also provided are chemical and chemo-enzymatic processes of preparing these paromomycin-derived aminoglycosides and intermediates thereof, as well as pharmaceutical compositions containing the same, and uses thereof in the treatment of genetic disorders.

The invention provides a method for mapping the location of the post-translational modifications of a post-translationally modified peptide. Also provided is a solid-phase support that includes a reagent for modifying a post-translationally modified amino acid residues of a post-translationally modified, converting it into a substrate for a peptidase.

The invention discloses the preparation of a tin dioxide-three-dimensional graphene (SnO2-3DGR) nanocomposite material immobilized protein-modified electrode and the application research of electrochemical sensing, which belongs to the technical field of biosensors. The method provided by the present invention is to prepare SnO2‑3DGR nanomaterials, which are drop-coated on the surface of ionic liquid carbon paste electrode (CILE), and then myoglobin (Mb) is drop-coated on the surface of CILE modified by SnO2‑3DGR. After natural drying, chitosan (CTS) solution was added dropwise and dried to prepare the third-generation electrochemical enzyme sensor. The sensor prepared by the invention has the characteristics of simple process, convenient operation, low cost, high detection sensitivity, strong electrical signal and simple electrode pretreatment, and can be used to detect organic small molecule trichloroacetic acid (TCA), with a detection limit of 1.67 mmol L -1.

The present invention provides a one-pot multi-enzyme method for preparing UDP-sugars from simple sugar starting materials. The invention also provides a one-pot multi-enzyme method for preparing oligosaccharides from simple sugar starting materials.

The present invention relates to a novel process for the preparation of optically enriched substituted R(+)beta-benzyl-gamma-butyrolactones of the general formula 1, wherein, R1 and R2 independently represent the following groups, R1=R2=H, OH, -OCnH2n+1 (n=1 to 8), NH2, and/or CF3, R1 and R2 together represents -O(CH2)mO- where m=2 to 4.

A process for preparing an intermediate for synthesizing escitalopram and the pharmaceutically acceptable salts thereof from 4-(4-dimethylamino)-1-(4′-fluorophenyl)-1-(hydroxybutyl)-3-(acyloxymethyl)benzonitrile is described. The process involves converting said intermediate into the (S+) enantiomer of citalopram by means of enzymatic enantiomeric resolution.

The present invention provides a chemical-enzymatic method for preparing D-serine. According to the method, DL-serine is adopted as a raw material and is derived into DL-N-phenylacetyl serine by using an acylating agent; immobilized penicillin acylase is adopted as a biocatalyst, correspondingly and selectively catalyze the DL-N-phenylacetyl serine in an aqueous medium to obtain L-serine, phenylacetic acid and D-N-phenylacetyl serine; a metal complexation method and an isoelectric point crystallization method are adopted to carry out separation to obtain optically pure D-phenylacetyl serine; the D-N-phenylacetyl serine is subjected to acid hydrolysis, concentration and crystallization to obtain the D-serine, wherein the yield of the D-serine is 45%, ee of the D-serine is 99.6%. The method provided by the present invention has characteristics of high yield, high chemical purity, high optical purity, environment-friendly property, and is suitable for the large-scale production of the D-serine.

The invention relates to an isolated culture method of chicken intestinal tract epithelial GammaDeltaT cells. Physical grinding is matched with a chemical enzyme process to dissociate chicken intestinal tract epithelial cells, thereby enhancing the intestinal tract cell dissociation degree, shortening the cell treatment time and ensuring the high activity of the cells; the use of the cell separation solution with the optimum density ensures the purification of the intestinal tract epithelial lymphocytes; the magnetic bead secondary antibody separation process is utilized to ensure the higher purity of the target cells; and the cell culture detects that the purity of the target cells obtained by isolated culture is up to 90%. The method overcomes the defects in the isolated culture process of the chicken intestinal tract epithelial GammaDeltaT cells, thereby laying the foundation for chicken intestinal tract epithelial GammaDeltaT cell culture and biological and immunological researches thereof.

The invention relates to an electrochemical enzyme-linked immunosensor, a preparation thereof and an application for detecting antigens, which belong to the technical field of protein detection. According to the preparation, an electrochemical enzyme-linked immunoprobe is prepared, the antigens are added into a biological conjugate of a magnetic bead and captured antibodies for incubation, an upper clear liquid is removed under the condition of an externally applied magnetic field, then the antigens are added into a biological conjugate of nano gold, detection antibodies and enzyme for incubation, and the electrochemical enzyme-linked immunoprobe is obtained after the double-antibody sandwich reaction; and a dispersion liquid of the immunoprobe is modified on the surface of an electrode ofa carbon paste base, so as to obtain the electrochemical enzyme-linked immunosensor. A linearity range of the electrochemical enzyme-linked immunosensor (MB-eElisa) provided by the invention is 0.01-6.0 ng mL<-1>, the detection limit is 4.0 pg mL<-1>(S/N=3); and compared with the existing electrochemical immunodetection method, the preparation method has low detection limit and high sensitivity.

The invention provides a photobiological hydrogen production system and a preparation method and application thereof. The preparation method comprises the following steps of: S1, mixing glucose, glucose oxidase, catalase and an inorganic green alga flocculating agent, so that gluconic acid generated by enzyme cascade reaction of glucose can spontaneously react with the inorganic green alga flocculating agent to form chemical-enzyme cascade reaction; and S2, introducing the chemical-enzyme cascade reaction into a green alga illumination culture system to flocculate the green alga to form greenalga aggregates, and keeping the green alga aggregates in an anaerobic and near-neutral pH environment for a long time to obtain a photobiological hydrogen production system, and monitoring the contents of oxygen and hydrogen in the photobiological hydrogen production system through a gas chromatograph. According to the invention, a simple, cheap, long-term and efficient photobiological hydrogen production system is provided, so that the problems that the anaerobic environment is difficult to maintain for a long time and green algae are easy to damage are solved, and the development of the application of photobiological hydrogen production in large-scale commercial hydrogen preparation is expected to be promoted.

The invention relates to a method for detecting mucoprotein type O-linked glycosylation (O-GalNAc type). The method is based on a strategy of combining biological enzyme selective oxidation and biological orthogonal chemical reaction; alactose 6-hydroxyl is oxidized into an aldehyde group by utilizing galactose oxidase, the glycopeptides are specifically captured and released through hydrazide chemistry, the O-GalNAc glycoform high sensitivity analysis is achieved by using the mass spectrometry-based detection method, and 59 O-GalNAc glycosylation modification sequences can be detected from 50[mu] L of human serum, and can correspondingly exceed 30 O glycated proteins. The method has the characteristics of high detection sensitivity, high enrichment specificity and the like, and is an important means for analyzing the existing endogenous O-GalNAc type glycosylated protein/peptide fragment.

A chemoenzymatic process for the production of fatty acid esters comprising partially esterifying one or more fatty acid esters, under mild temperatures with an enzymatic catalyst, and optionally in the presence of one or more inert solvents, with a 1- to 5-fold molar excess of one or more water-containing aliphatic alcohols with boiling points between 60° C. and 120° C., then removing the water and unreacted alcohol(s) from the resulting pre-esterification product, followed by additional esterification up to 99.7%, chemically-catalyzed with, e.g., an acid or tin salt at slightly higher temperatures, optionally using one or more inert solvents, with a 1- to 4-fold molar excess of the same one or more aliphatic alcohols as employed in the preliminary esterification step.