The invention discloses a preparation method and application of an anti-tumor immune
cell based on a ligand-targeted
cell conjugate (LTCC)technology. The preparation method comprises the following steps: adding non-natural
sugar modified with a biological orthogonal reaction group into a culture medium of immune cells, such as NK cells, so as to obtain immune cells modified with the biological orthogonal reaction group; then, modifying a targeting ligand, such as a nano
antibody, of which one end is a biological orthogonal reaction
pairing group capable of being matched with the biological orthogonal reaction group to generate a connection reaction to the surface of the immune
cell through biological orthogonal reaction under physiological conditions, wherein the linking mode is a transpeptidase SrtA mediated chemical
enzyme method, and ligand targeting has the characteristics of high-specificity recognition and high-expression
receptor binding to the surface of the tumor cell. The ligand-targeted modified immune cell can specifically bind to
cancer cells in a targeting manner, so that the modified immune cell generates an effect of secreting a large number of cytokines to enable the surfaces of the
cancer cells to generate transmembrane pores or phagocytic cleavage of the
cancer cells, and the immune cell has the effect of specifically killing the cancer cells.