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76 results about "Chemo enzymatic" patented technology

Bacterial transglycosylases: assays for monitoring the activity using Lipid II substrate analogs and methods for discovering new antibiotics

This invention provides a direct method for monitoring bacterial transglycosylase activity using labeled substrates produced by chemo-enzymatic synthesis wherein the labels are selected to permit the detection of both polymeric and non-polymeric products simultaneously, either directly or following the separation of product from starting material. The invention promotes the discovery of new antibiotics with activity against bacterial transglycosylases by a) laying the groundwork for structural analysis of purified, active transglycosylase (which permits structure-based design); and b) providing an assay that can be used to screen for inhibitors.
Owner:THE TRUSTEES FOR PRINCETON UNIV

Chemo-enzymatic synthesis of sialylated oligosaccharides

In vitro / cell-free process of preparing a sialylated oligosaccharides are described. The sialylated oligosaccharides include gangliosides. The oligosaccharides linked to various moieties including sphingoids and ceramides. Novel compounds that comprise sphingoid groups are disclosed. The compounds include sialylated oligosaccharides including gangliosides as well as various sphingoids and ceramides.
Owner:SENEB BIOSCI

Method for extracting isoflavone from kudzuvine root efficiently

The invention discloses a method for extracting isoflavone from kudzuvine root efficiently, and belongs to the technical field of the processing of agricultural products and food. The method comprises the following steps of: (1) crushing, namely crushing kudzuvine root to form 60 to 80-mesh kudzuvine root powder; (2) performing enzymolysis, namely adding 70 percent of ethanol into the kudzuvine root powder, adding cellulase and protease and performing the enzymolysis to obtain kudzuvine root extract, wherein 1 to 2 grams of the cellulase and the protease are added into each 100 milliliters of solution; and (3) extracting, namely performing ultrasonic-microwave processing on the obtained kudzuvine root extract from the step (2), concentrating under reduced pressure, freezing and drying to obtain kudzuvine root isoflavone. In the method, the kudzuvine root isoflavone is extracted by a compound enzymolysis technology of the cellulase and the protease and combining an ultrasonic-microwave assisted extraction technology, so the technical advantages of chemical enzymolysis and physical extraction are expressed fully, the cost is reduced, and the dissolution rate and extraction rate of the kudzuvine root isoflavone are improved, so that the extraction purity is greatly higher than that by conventional extraction method.
Owner:JIANSU MAOBAO GEYE CO LTD

Enhanced immunogenicity of tumor associated antigens by addition of alphaGal epitopes

The invention relates to methods and compositions for causing the selective targeting and killing of tumor cells. The present invention describes prophylactic or therapeutic cancer vaccines based on purified TAA proteins or TAA-derived synthetic peptides altered by chemical, enzymatic or chemo-enzymatic methods to introduce αGal epitopes or αGal glycomimetic epitopes, in order to allow for enhanced opsonization of the antigen by natural anti-αGal antibodies to stimulate TAA capture and presentation, thereby inducing a humoral and cellular immune response to the TAA expressed by a tumor. The animal's immune system thus is stimulated to produce tumor specific cytotoxic cells and antibodies which will attack and kill tumor cells present in the animal.
Owner:LUMOS PHARMA

A chemical enzymatic resolution preparation method of (r)-1-(1-naphthyl)ethylamine

The invention discloses a kinetic resolution method, in particular a method for preparing chemical enzyme of (R)-1-(1-naphthyl) ethylamine by resolution, which using 1-(1-naphthyl) ethylamine as a raw material. In the method, kinetic resolution is performed by using 1-(1-naphthyl) ethylamine which is a racemic mixture as a raw material, phenylethyl acetate as an acyl donor and immobilized lipase from Candida Antarctica as a catalyst under the controlled operation conditions that: the temperature is 30 to 80 DEG C, the volume ratio of the raw material to a solvent is 1:(25-50), the mass ratio of the raw material to the catalyst is 1:(0.02-0.05), and the molar ratio of the raw material to the phenylethyl acetate is 1:(1.2-4.0). The method has the advantages of high catalytic efficiency and environment friendliness.
Owner:倪发根

Methods for making simvastatin and intermediates

The invention provides synthetic chemical and chemoenzymatic methods of producing simvastatin and various intermediates. In one aspect, enzymes such as hydrolases, e.g., esterases, are used in the methods of the invention.
Owner:VERENIUM CORP (US)

Novel aminoglycosides and uses thereof in the treatment of genetic disorders

ActiveUS20090093418A1Improved termination mutation suppression activityLow toxicityBiocideMuscular disorderChemo enzymaticInosamycins
A new class of paromomycin-derived aminoglycosides, which exhibit efficient stop-codon mutation suppression activity, low toxicity and high selectivity towards eukaryotic cells are provided. Also provided are chemical and chemo-enzymatic processes of preparing these paromomycin-derived aminoglycosides and intermediates thereof, as well as pharmaceutical compositions containing the same, and uses thereof in the treatment of genetic disorders.
Owner:TECHNION RES & DEV FOUND LTD

Chemo-enzymatic process for proteome-wide mapping of post-translational modification

The invention provides a method for mapping the location of the post-translational modifications of a post-translationally modified peptide. Also provided is a solid-phase support that includes a reagent for modifying a post-translationally modified amino acid residues of a post-translationally modified, converting it into a substrate for a peptidase.
Owner:RGT UNIV OF CALIFORNIA

Preparation and electrochemical sensing application research of tin dioxide-three-dimensional graphene nanocomposite immobilized protein modified electrode

The invention discloses preparation and electrochemical sensing application research of a tin dioxide-three-dimensional graphene (SnO2-3DGR) nanocomposite immobilized protein modified electrode, and belongs to the technical field of biosensors. A method provided by the invention comprises the following steps: preparing a SnO2-3DGR nanometer material, dispensing the SnO2-3DGR nanometer material onto the surface of a carbon ionic liquid electrode (CILE), then dispensing myoglobin (Mb) onto the SnO2-3DGR modified CILE surface, naturally airing, dropwise adding a chitosan (CTS) solution, and drying a third-generation electrochemical enzyme sensor. The prepared sensor has the characteristics of a simple process, convenient operation, low cost, high detection sensitivity, strong electrical signal and simple electrode pretreatment, and can be used for detecting organic small molecular trichloroacetic acid (TCA), with a detection limit of 1.67 mmol / L.
Owner:HAINAN NORMAL UNIVERSITY

Bacterial transglycosylases: assays for monitoring the activity using Lipid II substrate analogs and methods for discovering new antibiotics

This invention provides a direct method for monitoring bacterial transglycosylase activity using labeled substrates produced by chemo-enzymatic synthesis wherein the labels are selected to permit the detection of both polymeric and non-polymeric products simultaneously, either directly or following the separation of product from starting material. The invention promotes the discovery of new antibiotics with activity against bacterial transglycosylases by a) laying the groundwork for structural analysis of purified, active transglycosylase (which permits structure-based design); and b) providing an assay that can be used to screen for inhibitors.
Owner:THE TRUSTEES FOR PRINCETON UNIV

Chemoenzymatic synthesis of peptide beta-lactones and beta-hydroxy acids

Methods of producing peptide beta-lactones and beta-hydroxy acids are disclosed that include contacting a beta-hydroxy-alpha-amino acid, an aryl carrier protein (ObiD), and ATP with a non-ribosomal protein synthetase. A continuous flow reactor is disclosed that includes an elongate conduit with at least one region that includes a first region with a non-ribosomal protein synthetase immobilized to a substrate. The non-ribosomal protein synthetase of the continuous flow reactor is configured to contact a flow of a reaction mixture that includes a beta-hydroxy-alpha-amino acid and an aryl carrier protein. The non-ribosomal protein synthetase is further configured to release a peptide beta-lactone into the flow of the reaction mixture.
Owner:WASHINGTON UNIV IN SAINT LOUIS

Glycosyltransferase reversibility for sugar nucleotide synthesis and microscale scanning

The present invention generally relates to materials and methods for exploiting glycosyltransferase reversibility for nucleotide diphosphate (NDP) sugar synthesis. The present invention provides engineered glycosyltransferase enzymes characterized by improved reaction reversibility and expanded sugar donor specificity as compared to corresponding non-mutated glycosyltransferase enzymes. Such reagents provide advantageous routes to NDP sugars for subsequent use in a variety of biomedical applications, including enzymatic and chemo-enzymatic glycorandomization.
Owner:WISCONSIN ALUMNI RES FOUND

Chemoenzymatic synthesis of heparin and heparan sulfate analogs

The present invention provides a one-pot multi-enzyme method for preparing UDP-sugars from simple sugar starting materials. The invention also provides a one-pot multi-enzyme method for preparing oligosaccharides from simple sugar starting materials.
Owner:RGT UNIV OF CALIFORNIA

Bacterial transglycosylases: assays for monitoring the activity using Lipid II substrate analogs and methods for discovering new antibiotics

This invention provides a direct method for monitoring bacterial transglycosylase activity using labeled substrates produced by chemo-enzymatic synthesis wherein the labels are selected to permit the detection of both polymeric and non-polymeric products simultaneously, either directly or following the separation of product from starting material. The invention promotes the discovery of new antibiotics with activity against bacterial transglycosylases by a) laying the groundwork for structural analysis of purified, active transglycosylase (which permits structure-based design); and b) providing an assay that can be used to screen for inhibitors.
Owner:THE TRUSTEES OF PRINCETON UNIV

Capillary electrophoresis electrochemical enzyme linked immunoassay for detecting amnesic shellfish toxicity

The invention discloses capillary electrophoresis electrochemical enzyme linked immunoassay for detecting amnesic shellfish toxicity, which belongs to the technical field of assay and detection. Solution of standard substances and shellfish samples with the amnesic shellfish toxicity are assayed by combining the capillary electrophoresis technology and electrochemical enzyme linked immunoassay technology. The capillary electrophoresis electrochemical enzyme linked immunoassay comprises the following steps of: reacting sample solution and an amnesic shellfish toxicity antibody marked by a horse radish peroxidase (HRP) in a noncompetitive mode; separating an amnesic shellfish toxicity-enzyme labeled antibody compound and the rest amnesic shellfish toxicity antibody marked by the HRP in the mixed solution by capillary electrophoresis; generating a 3-aminophenazine with electrochemical activities by using aminophenol which is the oxidogenic substrate of peroxide in the presence of a catalyst; and performing electrochemical detection. The method simplifies a sample treatment process and has high selectivity and high accuracy. The linear detection range of the solution of amnesic shellfish toxicity standard substances of the method is 0.5 to 50ng / mL and a detection limit of the method is 0.2ng / mL. The method is an ideal method for detecting the amnesic shellfish toxicity of shellfish samples.
Owner:QINGDAO UNIV OF SCI & TECH

Chemical-enzymatic method for preparing D-serine

The present invention provides a chemical-enzymatic method for preparing D-serine. According to the method, DL-serine is adopted as a raw material and is derived into DL-N-phenylacetyl serine by using an acylating agent; immobilized penicillin acylase is adopted as a biocatalyst, correspondingly and selectively catalyze the DL-N-phenylacetyl serine in an aqueous medium to obtain L-serine, phenylacetic acid and D-N-phenylacetyl serine; a metal complexation method and an isoelectric point crystallization method are adopted to carry out separation to obtain optically pure D-phenylacetyl serine; the D-N-phenylacetyl serine is subjected to acid hydrolysis, concentration and crystallization to obtain the D-serine, wherein the yield of the D-serine is 45%, ee of the D-serine is 99.6%. The method provided by the present invention has characteristics of high yield, high chemical purity, high optical purity, environment-friendly property, and is suitable for the large-scale production of the D-serine.
Owner:CHONGQING UNIV OF POSTS & TELECOMM

Isolated culture method of chicken intestinal tract epithelial GammaDeltaT cells

The invention relates to an isolated culture method of chicken intestinal tract epithelial GammaDeltaT cells. Physical grinding is matched with a chemical enzyme process to dissociate chicken intestinal tract epithelial cells, thereby enhancing the intestinal tract cell dissociation degree, shortening the cell treatment time and ensuring the high activity of the cells; the use of the cell separation solution with the optimum density ensures the purification of the intestinal tract epithelial lymphocytes; the magnetic bead secondary antibody separation process is utilized to ensure the higher purity of the target cells; and the cell culture detects that the purity of the target cells obtained by isolated culture is up to 90%. The method overcomes the defects in the isolated culture process of the chicken intestinal tract epithelial GammaDeltaT cells, thereby laying the foundation for chicken intestinal tract epithelial GammaDeltaT cell culture and biological and immunological researches thereof.
Owner:FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES

Chemical-enzyme method for preparing D-basic amino acid hydrochloride

InactiveCN102392061AGuaranteed high optical activitySimpler than ion exchange separationOrganic compound preparationAmino-carboxyl compound preparationPenicillinPhenylacetic acid
The invention provides a chemical-enzyme method for preparing D-basic amino acid hydrochloride. The method comprises the following steps of: derivatizing DL-basic amino acid serving as a raw material to obtain DL-N-diphenyl acetyl basic amino acid by utilizing an acylating agent, hydrolyzing the DL-N-diphenyl acetyl basic amino acid by using immobilized penicillin acylase as a biocatalyst through non-stereo selectivity and enantioselectivity to obtain L-basic amino acid, phenylacetic acid and D-alpha-N-phenylacetyl basic amino acid; and performing acidolysis on the D-alpha-N-phenylacetyl basic amino acid to obtain phenylacetic acid and D-basic amino acid dihydrochloride, and performing treatment of epoxypropane to obtain the D-basic amino acid hydrochloride. The D-lysine hydrochloride and D-ornithine hydrochloride which are prepared by the method have the yield of more than 40 percent, and ee is more than 99 percent. The method has the characteristics of high yield and optical purity, simple process and the like, and is suitable for the large-scale production of the D-basic amino acid hydrochloride.
Owner:CHONGQING UNIV OF POSTS & TELECOMM

Electrochemical enzyme-linked immunosensor, preparation thereof and application for detecting antigens

ActiveCN110376380ASolve problems such as being easily inactivated by proteinsImprove accuracyMaterial electrochemical variablesAntigenProtein detection
The invention relates to an electrochemical enzyme-linked immunosensor, a preparation thereof and an application for detecting antigens, which belong to the technical field of protein detection. According to the preparation, an electrochemical enzyme-linked immunoprobe is prepared, the antigens are added into a biological conjugate of a magnetic bead and captured antibodies for incubation, an upper clear liquid is removed under the condition of an externally applied magnetic field, then the antigens are added into a biological conjugate of nano gold, detection antibodies and enzyme for incubation, and the electrochemical enzyme-linked immunoprobe is obtained after the double-antibody sandwich reaction; and a dispersion liquid of the immunoprobe is modified on the surface of an electrode ofa carbon paste base, so as to obtain the electrochemical enzyme-linked immunosensor. A linearity range of the electrochemical enzyme-linked immunosensor (MB-eElisa) provided by the invention is 0.01-6.0 ng mL<-1>, the detection limit is 4.0 pg mL<-1>(S / N=3); and compared with the existing electrochemical immunodetection method, the preparation method has low detection limit and high sensitivity.
Owner:HUAZHONG UNIV OF SCI & TECH

Method for synthesizing pseudouridine by chemical enzyme method

The invention discloses a method for synthesizing pseudouridine by a chemical enzyme method, which comprises the following steps: synthesizing pseudouridine monophosphate from ribose-5-phosphoric acid and a pseudouridine-5-phosphosidase mutant, and carrying out dephosphorylation and separation to obtain pseudouridine. The amino acid sequence of the pseudouridine-5-phosphosidase mutant is as shown in SEQ ID NO: 1. According to the method, the pseudouridine is synthesized through chemical-enzymatic reaction, mass production of the pseudouridine is realized through simple reaction and low cost, and the application of industrial synthesis of the pseudouridine is promoted.
Owner:武汉糖智药业有限公司

Photobiological hydrogen production system and preparation method and application thereof

The invention provides a photobiological hydrogen production system and a preparation method and application thereof. The preparation method comprises the following steps of: S1, mixing glucose, glucose oxidase, catalase and an inorganic green alga flocculating agent, so that gluconic acid generated by enzyme cascade reaction of glucose can spontaneously react with the inorganic green alga flocculating agent to form chemical-enzyme cascade reaction; and S2, introducing the chemical-enzyme cascade reaction into a green alga illumination culture system to flocculate the green alga to form greenalga aggregates, and keeping the green alga aggregates in an anaerobic and near-neutral pH environment for a long time to obtain a photobiological hydrogen production system, and monitoring the contents of oxygen and hydrogen in the photobiological hydrogen production system through a gas chromatograph. According to the invention, a simple, cheap, long-term and efficient photobiological hydrogen production system is provided, so that the problems that the anaerobic environment is difficult to maintain for a long time and green algae are easy to damage are solved, and the development of the application of photobiological hydrogen production in large-scale commercial hydrogen preparation is expected to be promoted.
Owner:SHANGHAI JIAO TONG UNIV

Chemical-enzyme method for preparing D-tyrosine

ActiveCN102352392AAvoid formingSimplify catalytic reactionsFermentationPenicillinPhenylacetic acid
The invention provides a chemical-enzyme method for preparing D-tyrosine. The method comprises the following steps: based on DL-tyrosine as a raw material, deriving DL-tyrosine into DL-N-phenylacetyltyrosine in an acidic medium by using an acylating agent; based on immobilized penicillin acylase as a biocatalyst, carrying out enantiomorphous selective hydrolysis so as to form L-tyrosine, phenylacetic acid and D-N-phenylacetyltyrosine; and carrying out acidolysis on D-N-phenylacetyltyrosine so as to obtain phenylacetic acid and D-tyrosine. According to the chemical-enzyme method, the yield of D-tyrosine is 40%, and enantiomeric excess is larger than 99%. The method provided by the invention has the characteristics of high yield and optical purity of product, simple process and the like, and is suitable for large-scale production of D-tyrosine.
Owner:CHONGQING UNIV OF POSTS & TELECOMM

Anti-tumor immune cell based on ligand-targeted cell conjugate (LTCC) technology as well as preparation method and application of anti-tumor immune cell

InactiveCN112675202AImprove the ability of targeting and killing tumorsImprove securityCulture processMammal material medical ingredientsCancer cellAntiendomysial antibodies
The invention discloses a preparation method and application of an anti-tumor immune cell based on a ligand-targeted cell conjugate (LTCC)technology. The preparation method comprises the following steps: adding non-natural sugar modified with a biological orthogonal reaction group into a culture medium of immune cells, such as NK cells, so as to obtain immune cells modified with the biological orthogonal reaction group; then, modifying a targeting ligand, such as a nano antibody, of which one end is a biological orthogonal reaction pairing group capable of being matched with the biological orthogonal reaction group to generate a connection reaction to the surface of the immune cell through biological orthogonal reaction under physiological conditions, wherein the linking mode is a transpeptidase SrtA mediated chemical enzyme method, and ligand targeting has the characteristics of high-specificity recognition and high-expression receptor binding to the surface of the tumor cell. The ligand-targeted modified immune cell can specifically bind to cancer cells in a targeting manner, so that the modified immune cell generates an effect of secreting a large number of cytokines to enable the surfaces of the cancer cells to generate transmembrane pores or phagocytic cleavage of the cancer cells, and the immune cell has the effect of specifically killing the cancer cells.
Owner:AFFILIATED HOSPITAL OF JIANGNAN UNIV +1

Enhanced immunogenicity of tumor associated antigens by addition of alphaGal epitopes

The invention relates to methods and compositions for causing the selective targeting and killing of tumor cells. The present invention describes prophylactic or therapeutic cancer vaccines based on purified TAA proteins or TAA-derived synthetic peptides altered by chemical, enzymatic or chemo-enzymatic methods to introduce αGal epitopes or αGal glycomimetic epitopes, in order to allow for enhanced opsonization of the antigen by natural anti-αGal antibodies to stimulate TAA capture and presentation, thereby inducing a humoral and cellular immune response to the TAA expressed by a tumor. The animal's immune system thus is stimulated to produce tumor specific cytotoxic cells and antibodies which will attack and kill tumor cells present in the animal.
Owner:LUMOS PHARMA

Human serum O-glycosylation identification method based on chemical enzymatic catalysis

The invention relates to a method for detecting mucoprotein type O-linked glycosylation (O-GalNAc type). The method is based on a strategy of combining biological enzyme selective oxidation and biological orthogonal chemical reaction; alactose 6-hydroxyl is oxidized into an aldehyde group by utilizing galactose oxidase, the glycopeptides are specifically captured and released through hydrazide chemistry, the O-GalNAc glycoform high sensitivity analysis is achieved by using the mass spectrometry-based detection method, and 59 O-GalNAc glycosylation modification sequences can be detected from 50[mu] L of human serum, and can correspondingly exceed 30 O glycated proteins. The method has the characteristics of high detection sensitivity, high enrichment specificity and the like, and is an important means for analyzing the existing endogenous O-GalNAc type glycosylated protein / peptide fragment.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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