Photobiological hydrogen production system and preparation method and application thereof
A photobiological and systematic technology, applied in the field of photobiological hydrogen production system and its preparation, can solve the problems of difficult long-term maintenance of anaerobic environment and easy damage of green algae, and achieve good mitochondrial activity, high cell activity, and efficient removal. effect of oxygen
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[0034] To prepare a simple, cheap and long-term efficient photobiological hydrogen production system, including the following steps:
[0035] Mix 1mg of glucose oxidase (total enzyme activity about 50U) and 1mg of catalase (total enzyme activity about 50KU) into a 1.5mL graduated EP tube, add 1ml of phosphate buffer to dissolve, seal the EP tube, and put it in 4 Store under ℃ conditions. Weigh 27mg of glucose and 8.75mg of magnesium hydroxide respectively, place them in a transparent glass test tube with a volume of 8mL, then add 2mL of Chlamydomonas reinhardtii liquid culture (OD750=0.5), then add 1mL of glucose oxidase and catalase Premix the solution, seal the test tube and culture at 25°C under 6300lux light conditions, draw 10 μL of liquid culture with an airtight needle at fixed time points (2 days, 6 days, 10 days, 14 days, 18 days, 22 days, 26 days) The substance was dropped on a glass slide, covered with a cover glass, and observed under a Zeiss fluorescence microsco...
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