Photobiological hydrogen production system and preparation method and application thereof

A photobiological and systematic technology, applied in the field of photobiological hydrogen production system and its preparation, can solve the problems of difficult long-term maintenance of anaerobic environment and easy damage of green algae, and achieve good mitochondrial activity, high cell activity, and efficient removal. effect of oxygen

Active Publication Date: 2020-06-12
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0005] The purpose of the present invention is to provide a photobiological hydrogen production system and its preparation method and application, so as to solve the problems in the prior art that the anaerobic environment is difficult to maintain for a long time and green algae are easily damaged in the photobiological hydrogen production system

Method used

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  • Photobiological hydrogen production system and preparation method and application thereof
  • Photobiological hydrogen production system and preparation method and application thereof
  • Photobiological hydrogen production system and preparation method and application thereof

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Embodiment 1

[0034] To prepare a simple, cheap and long-term efficient photobiological hydrogen production system, including the following steps:

[0035] Mix 1mg of glucose oxidase (total enzyme activity about 50U) and 1mg of catalase (total enzyme activity about 50KU) into a 1.5mL graduated EP tube, add 1ml of phosphate buffer to dissolve, seal the EP tube, and put it in 4 Store under ℃ conditions. Weigh 27mg of glucose and 8.75mg of magnesium hydroxide respectively, place them in a transparent glass test tube with a volume of 8mL, then add 2mL of Chlamydomonas reinhardtii liquid culture (OD750=0.5), then add 1mL of glucose oxidase and catalase Premix the solution, seal the test tube and culture at 25°C under 6300lux light conditions, draw 10 μL of liquid culture with an airtight needle at fixed time points (2 days, 6 days, 10 days, 14 days, 18 days, 22 days, 26 days) The substance was dropped on a glass slide, covered with a cover glass, and observed under a Zeiss fluorescence microsco...

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Abstract

The invention provides a photobiological hydrogen production system and a preparation method and application thereof. The preparation method comprises the following steps of: S1, mixing glucose, glucose oxidase, catalase and an inorganic green alga flocculating agent, so that gluconic acid generated by enzyme cascade reaction of glucose can spontaneously react with the inorganic green alga flocculating agent to form chemical-enzyme cascade reaction; and S2, introducing the chemical-enzyme cascade reaction into a green alga illumination culture system to flocculate the green alga to form greenalga aggregates, and keeping the green alga aggregates in an anaerobic and near-neutral pH environment for a long time to obtain a photobiological hydrogen production system, and monitoring the contents of oxygen and hydrogen in the photobiological hydrogen production system through a gas chromatograph. According to the invention, a simple, cheap, long-term and efficient photobiological hydrogen production system is provided, so that the problems that the anaerobic environment is difficult to maintain for a long time and green algae are easy to damage are solved, and the development of the application of photobiological hydrogen production in large-scale commercial hydrogen preparation is expected to be promoted.

Description

technical field [0001] The invention relates to the field of photobiological hydrogen production, and more specifically relates to a photobiological hydrogen production system and its preparation method and application. Background technique [0002] With the gradual depletion of traditional fossil energy materials such as petroleum and the global environmental pollution it brings, it is urgent to develop green and environmentally friendly new energy materials as an alternative. Recently, hydrogen has attracted more and more attention as a renewable, clean, and efficient energy source. However, the hydrogen currently available for commercialization still comes from traditional energy sources such as natural gas, which has been controversial due to environmental pollution and low efficiency. . [0003] Photobiological hydrogen production, because it directly uses solar energy as an energy source, and uses renewable organisms or biological enzymes as a catalyst, is the most pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00
CPCC12P3/00
Inventor 樊春海柳华杰王丽华陈杰李茜
Owner SHANGHAI JIAO TONG UNIV
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