166 results about "Cascade reaction" patented technology

The invention discloses a biosynthesis system for high-yield 1, 6-hexamethylenediamine and application of the biosynthesis system, and belongs to the technical field of biology. According to the method, key genes which are excavated and optimized in a 1, 6-hexamethylenediamine biosynthetic pathway are co-expressed in a single cell module or a multi-cell module, and different enzyme elements expressed by the cell module are subjected to enzyme cascade reaction, so that 1, 6-hexamethylenediamine can be efficiently synthesized by taking caprolactam or 6-aminocaproic acid as a substrate; furthermore, after the carboxylic acid reductase MaCAR is mutated, the catalytic activity of the carboxylic acid reductase MaCAR can be remarkably improved, and the yield of the 1, 6-hexamethylenediamine is further improved; in addition, the biosynthesis system disclosed by the invention directly takes the cell module as a whole-cell catalyst, and an enzyme purification step is avoided, so that the biosynthesis system has the advantage of low cost.

The invention discloses a method for preparing high-value chemicals through photocatalytic driving of beta-O-4 lignin depolymerization and coupling cascade reaction, and belongs to the technical field of photocatalytic biomass resource conversion. The method comprises the following steps: (1) dispersing a beta-O-4 lignin raw material into a solvent containing a photocatalyst; (2) introducing a normal-temperature chemically stable gas to replace air in a reaction system; and (3) carrying out a depolymerization reaction of beta-O-4 bonds and an in-situ C-C bond coupling reaction under an illumination condition to generate a target product. And (4) separating and purifying the product and recovering the catalyst. Through photocatalytic cascade reaction design, the defects of harsh reaction conditions and multi-step separation in a traditional method are avoided, the method has the advantages of mild reaction conditions, high atom utilization rate, high product selectivity and environmental friendliness, and a new way is provided for high-value utilization of lignin.

The invention discloses a method for efficiently producing pyridoxal phosphate based on a three-enzyme cascade reaction and a recombinant strain, and belongs to the technical field of biological engineering. The recombinant escherichia coli for co-expressing the pyridoxine phosphate phosphatase mutant, the pyridoxine phosphate oxidase and the hydrogen peroxide decomposing enzyme is constructed, three-enzyme synergy is realized by optimizing the copy number of a carrier, and the catalytic efficiency of a recombinant strain as a cell catalyst is improved. The invention further provides a one-pot method for preparing pyridoxal phosphate, the production cost is reduced, an efficient, green and economical biosynthesis path is provided for industrial production of PLP, and the method can be widely applied to the fields of food, medicine and the like and has important industrial value and application prospects.

This invention discloses a method for synthesizing Rotundine and its derivatives, as well as the construction of a whole-cell catalytic system. This invention utilizes an artificially designed multi-enzyme cascade reaction method to efficiently synthesize Rotundine and its derivatives. Furthermore, this invention constructs the enzymes of the synthetic route into E. coli chassis cells to obtain a whole-cell catalyst comprising M1a, M2a, M3a, and M4a. Using the whole-cell catalyst to synthesize Rotundine and its derivatives, the yield of Rotundine can reach 2.44 g / L.

A fluidized bed device for coupling naphtha and methanol to prepare aromatics and co-produce olefins and its application method are provided. By using the device, under the action of a catalyst, naphtha reacts with methanol to generate product gas containing aromatics and light olefins as main components. The method can efficiently and selectively convert linear and branched aliphatic hydrocarbons into aromatics, while also increasing p-xylene production through aromatic methylation reactions, with the p-xylene content in the xylene mixture exceeding 75 wt %. The fluidized bed reactor achieves increased p-xylene production by controlling the progression of cascade reactions (naphtha→benzene / toluene→p-xylene). Additionally, it utilizes the methylation reaction of benzene / toluene with methanol to provide in-situ heat for the coupled naphtha-methanol aromatization process, thereby achieving autothermal balance.

This invention discloses a method for preparing sulfonamide-modified benzo[a]fluorene-5-one derivatives, using 1,7-enyne and N Using sulfonylaminopyridine salts as raw materials, a series of sulfonated benzo[a]fluorene-5-one compounds were successfully synthesized by catalyzing the sulfonation / cyclization tandem reaction of 1,7-enynes under photocatalytic conditions. This method has excellent functional group tolerance and high economic applicability.

The present application relates to the technical field of organic synthesis, and particularly relates to a method for preparing (+)-crispine A by means of biological enzyme resolution. First, 6,7-dimethoxy-3,4-dihydroisoquinoline-2-oxide is subjected to 1,3-dipolar cycloaddition with allyl alcohol to obtain a first compound; then the first compound is added into a sulfonylation reagent and zinc powder to obtain a racemate second compound through a three-step continuous series reaction of primary alcohol sulfonylation, nitrogen-oxygen bond cleavage and ring formation; then the second compound is mixed with vinyl acetate and biological enzyme, and chiral resolution is carried out under the action of the biological enzyme to obtain a third compound with right-handed optical rotation and a fourth compound with left-handed optical rotation; finally, the third compound is mixed with an alkaline reagent and a sulfonylation reagent, and then secondary alcohol sulfonylation is carried out to obtain a fifth compound with right-handed optical rotation; the fifth compound is mixed with a deoxidizing reagent to carry out deoxidation reaction, and (+)-crispine A is prepared. The method is simple, green and environmentally friendly, and can be produced in large quantities.

The invention develops a cascade reaction strategy for realizing efficient synthesis of a caged ketal skeleton compound by taking Bronsted acid as a catalyst and taking a 3-hexene-2, 5-diketone derivative and a cinnamyl alcohol analogue as substrates, and synthesizes a natural product (+ / -)-catunaregin and a series of novel analogues of the natural product (+ / -)-catunaregin which are not reported in literatures. Important technical support and early-stage theoretical basis are provided for the creation of new drugs of the compounds. The cinnamyl alcohol analogue disclosed by the invention is simple and easy to prepare, wide in derivation range and low in price, and can better enrich the structural diversity of caged ketal skeleton compounds. The method disclosed by the invention can be prepared by a one-pot method, is efficient and rapid, simple to operate, short in reaction step, good in substrate tolerance, mild in reaction condition and wide in application range, and overcomes the defects of complex operation, harsh reaction conditions, substrate limitation, intolerant substrate functional groups, tedious steps and the like in the traditional reaction.

Disclosed in the present invention are a cascade reaction device and method for preparing aviation fuel by means of photoelectrocatalytic conversion of lignin. The method comprises: placing lignin in a pretreatment reaction chamber filled with an alkaline solution, so that the lignin is fully dissolved; opening valves to enable a pretreated reaction liquid to flow into an anode chamber in a photoelectrocatalytic reaction chamber; using solar energy to drive a photoelectrocatalytic reaction at an anode; after sufficient reaction, enabling the reaction liquid in the anode chamber to flow into a temporary storage chamber, and subsequently, using a pump to transfer the reaction liquid in the temporary storage chamber into a cathode chamber; and after sufficient reaction, introducing the reaction liquid in the cathode chamber into a high-pressure reaction chamber by means of a pump, and enabling hydrogen generated in the cathode chamber to flow into a gas cylinder by means of a pipe for collection, so as to be supplied to the high-pressure reaction chamber, wherein the reaction liquid undergoes a hydrodeoxygenation reaction under the action of a catalyst in the high-pressure reaction chamber, and after sufficient reaction, the aviation fuel can be obtained. The present invention achieves an integrated cascade reaction for solar-driven conversion of lignin into aviation fuel.

The invention relates to a process device and method for preparing a carbon nano tube based on serial connection of methane dry reforming and Fischer-Tropsch synthesis reaction, and belongs to the technical field of carbon nano material preparation and chemical processes. The invention provides a process device and method for preparing a carbon nanotube based on serial connection of methane dry reforming and Fischer-Tropsch synthesis reaction, a thermal-thermal serial connection strategy is adopted, and a dual-catalyst-dual-bed coupling serial connection system of a fixed bed (DRM reaction unit) and a tubular furnace (FTS reaction unit) is used for preparing the carbon nanotube. The invention provides a process device for preparing a carbon nanotube based on serial connection of methane dry reforming and Fischer-Tropsch synthesis reaction. The process device mainly comprises a serial connection reaction group and an online detection unit which are connected in sequence.

The application discloses a five-membered ring containing alkynyl or triazole functional group P The chiral phosphine oxide compound is obtained through Domino Heck-Sonogashira cascade reaction by taking a prochiral tertiary phosphine oxide compound containing a double ethylene group and phenylacetylene as raw materials, transition metal palladium catalyst and chiral TADDOL phosphoramidite ligand under the protection of inert gas P The chiral phosphine oxide compound has a product yield of 96% and an enantioselectivity ee value of 99%, and a diastereoselectivity dr value of greater than 20:1. The application adopts an asymmetric desymmetrization strategy, uses a Heck reaction to start and a Sonogashira reaction to end a cascade reaction, simultaneously constructs P The chiral and quaternary carbon chiral center has mild reaction conditions, simple operation and good substrate universality, provides a compound skeleton with diversity, and is successfully applied to a palladium-catalyzed asymmetric allyl substitution reaction as a chiral ligand.

The invention provides a synthesis method and application of a tetra-substituted pyran compound. The tetra-substituted pyran compound is directly constructed through a one-pot cascade reaction under an organic base catalysis system by taking triketone olefin and allyl ammonium salt as key reaction raw materials. The method has the advantages that the operation is simple and convenient, no water or oxygen is needed, the reaction is efficient, the steps are simple, the method is environment-friendly, the reaction is easy to amplify, and the method has practical value. Activity tests prove that the compounds have certain inhibitory activity on alpha-glucosidase, or can be used for treating diabetes mellitus.

This application discloses a method for synthesizing chiral cyano-containing full-carbon quaternary cyclic compounds, characterized by the following steps: mixing a solvent, an oxidant, an alkynyl malononitrile compound, a carboxylic acid compound, a palladium salt, and a chiral dinitrogen ligand; reacting the mixture; drying; and separating to obtain the chiral cyano-containing full-carbon quaternary cyclic compound. This method can achieve a palladium-catalyzed tandem reaction of alkynyl malononitrile with a carboxylic acid involving asymmetric acyl oxidative cyclization / acyl migration, yielding cyano-containing full-carbon quaternary cyclic compounds with optical purity in good yields (enantiomer ratios up to 96.5:3.5). This invention features a broad substrate range, readily available raw materials, simple and practical operation, and mild reaction conditions.

This invention provides a method for preparing 4,1-disubstituted pyrrolo[1,2-a]quinoxaline-1-yl ketone derivatives, belonging to the field of organic synthesis technology. This invention uses 2-(1H-pyrrolo-1-yl)aniline compounds and α-carbonyl acids as reactants, employing only ammonium persulfate as an oxidant. Under metal-free catalysis, a one-pot tandem reaction is carried out to simultaneously complete the cyclization of the pyrrolo[1,2-a]quinoxaline nucleus and the C-1 acylation modification. The substituents at the 4- and 1-positions of the product can be flexibly controlled by changing the α-carbonyl acid with different structures. This method requires no pre-modified substrate, eliminates the need for metal catalysts, avoids the risk of metal residue, and features simplified steps and high functional group compatibility, providing a new green and low-cost route for the synthesis of polysubstituted pyrrolo[1,2-a]quinoxaline ketone derivatives.

The invention provides a metal-heteropolyacid catalyst confined to a molecular sieve as well as a preparation method and hydrodeoxygenation application of the metal-heteropolyacid catalyst. The catalyst takes a spherical pure silicon molecular sieve with the particle size of 2-3mm as a carrier, and transition metal and heteropoly acid active components are supported in a pore channel in a limited range; the mass ratio of the heteropoly acid to the metal is (10-50): 1. According to the invention, an organic amine bridging strategy is adopted to construct near double active sites, that is, organic amine molecules in heteropolyacid organic amine salt are used as a guiding anchoring agent, and a transition metal precursor is accurately anchored near heteropolyacid. After roasting, a concerted catalysis center in which a metal hydrogenation site and a heteropolyacid deoxidation site are closely close on the nanoscale is successfully constructed in a confinement space of the molecular sieve, and the mass transfer efficiency and the step coupling effect in a hydrodeoxygenation multi-step cascade reaction are improved. When the weight hourly space velocity is 2 g / gcat. / h, the temperature is 150-250 DEG C and the pressure is 0.5-2 MPa, the conversion rate of the oxygen-enriched phenolic compound can be greater than or equal to 94%, and the yield of cycloalkane can be greater than or equal to 90%.

This invention discloses an α-indolylpyrrolo[1,2-a]indole derivative and its preparation method. The derivative is a levorotatory or dextrorotatory optically active form or racemate with the following structural formula: The preparation involves using α-indolylpropynyl alcohols and indole compounds as raw materials, and 3,5-bis(trifluoromethyl)phenylbinaphthylphosphonic acid as a catalyst, reacting in an organic solvent to obtain the α-indolylpyrrolo[1,2-a]indole derivative. This invention utilizes an organophosphate-catalyzed tandem reaction of two components to synthesize the α-indolylpyrrolo[1,2-a]indole derivative. The reaction conditions are mild, the process is simple, and the operation is convenient. The obtained product is the core skeleton of the indole bases of the natural products isoborreverine and dimethyl isoborreverine, which is of great significance for the synthesis of analogs of these natural products.

The application is suitable for the field of analog enzyme technology, and provides a light-controlled zein-based dual-enzyme-activity analog enzyme and a preparation method thereof.The preparation method comprises the following steps: under water bath conditions, dissolving zein in an alkaline aqueous solution and incubating; adding a hydroxylamine solution to the solution obtained in step S1 and continuing to react; and after the reaction is completed, obtaining the light-controlled zein-based dual-enzyme-activity analog enzyme.The application uses zein as a raw material, is modified by hydroxylamine, does not need any metal component to participate, has light-controlled switchable dual-enzyme activity, exhibits oxidase-like activity under visible light irradiation, presents peroxidase-like activity in dark conditions, and can realize reversible switching of the two enzyme activities by regulating light, effectively overcomes the problems that traditional artificial analog enzymes depend on metal ions, multiple enzyme activities coexist, interfere with each other and are difficult to accurately control, can be used for colorimetric detection of total antioxidant capacity, can be used for colorimetric detection of glucose in cascade reaction with glucose oxidase, has the advantages of simple operation, rapid response, high sensitivity, good selectivity, low cost and the like, and has a good application prospect in the fields of food analysis, clinical diagnosis and the like.

The invention discloses a method for synthesizing mesalazine and application of mesalazine. The method comprises the following steps: introducing p-nitrophenol, formic acid and a Cu-Co-coated MFI-NS molecular sieve catalyst into a continuous flow reaction zone, and sequentially carrying out electrophilic substitution and nitro hydrogenation cascade reaction at normal pressure and 50-70 DEG C; the catalyst has a micropore-mesopore stepped pore channel, and contains a Cu0 metal cluster and a framework Co < 2 + > acid site. And separating, acidifying and crystallizing the reaction liquid to obtain a finished product. According to the invention, the shape-selective selectivity and difunctional synergistic effect of the molecular sieve are utilized, high conversion rate (gt, 98%) and high selectivity (gt, 94%) under mild conditions are realized, the product purity is greater than or equal to 99.5%, and the Cu / Co residue meets the ICHQ3D standard. The catalyst can be recycled for 10 times or more, the yield is reduced by 5% or less, iron mud pollution of a traditional iron powder method and the metal leaching risk of a noble metal catalyst are thoroughly eliminated, and the method has the remarkable advantages of being environmentally friendly, efficient, low in cost, easy to industrially amplify and the like and is suitable for preparing the medicine for treating the inflammatory bowel disease.

This application discloses a multi-enzyme complex based on a Snap-Fit self-assembly tag, its construction method, and its applications, relating to the field of protein engineering technology. The multi-enzyme complex based on the Snap-Fit self-assembly tag comprises: a TLL-FitC fusion protein with the amino acid sequence shown in SEQ ID NO.13, a FitN-AnChOx fusion protein with the amino acid sequence shown in SEQ ID NO.14, and an OleT protein with the amino acid sequence shown in SEQ ID NO.15. PRN - SnapG fusion protein. This multi-enzyme complex employs a Snap-Fit assembly strategy, which allows three different enzymes to be assembled using only one set of tags. When applied to the synthesis of olefins, it helps to improve the transfer efficiency of 1-olefin cascade reaction intermediates between upstream and downstream enzyme active sites, thereby increasing the final yield, and has great application potential.

The application belongs to the technical field of aniline compound preparation, and particularly relates to a synthesis method of deuterated aniline based on transition metal catalyzed nitrobenzene compound. The method uses nitrobenzene compound as raw material, is catalyzed by a low-load transition metal catalyst, and adopts one-pot method to complete the series reaction of high-selectivity hydrogenation and hydrogen-deuterium exchange of the nitrobenzene compound, so that the deuterated aniline compound with high added value is obtained. The yield of the deuterated product and the deuterium substitution rate of the product are better than those of conventional hydrogenation and deuterium substitution, the product is stable and cannot be easily replaced by hydrogen to return to the raw material, and the operation and application are convenient.

The application relates to the field of slow-release fertilizers, and particularly discloses a nano-selenium drought-resistant and high-temperature-resistant slow-release fertilizer and a preparation method thereof. 0 The method comprises the following steps: fixing glucose oxidase on the surface of TiO2 nanoparticles, and performing cascade reaction of the glucose oxidase, glucose and sodium selenite under mild conditions to realize synchronous synthesis of Se The method is green, mild and controllable, the obtained selenium fertilizer has excellent stability and slow-release performance, can significantly improve the selenium content and yield of crops, and is suitable for selenium-rich functional agriculture.

The application provides a preparation method and a production system for preparing dichloropropanol by glycerol chlorination, and belongs to the technical field of compound preparation. The method adopts multistage series connection of reaction kettles for reaction, and precisely controls the chlorination reaction process by controlling the reaction temperature and the dehydration amount. In the reaction, hydrogen chloride gas is kept in communication with all the reactors, glycerol and a catalyst are fed into a first-stage reaction kettle for reaction, liquid-phase products are transferred into a next-stage reaction kettle for continuous reaction, and gas-phase products are transferred into the original reaction kettle for continuous reaction after dehydration. The method provided by the application can not only precisely and efficiently solve the water inhibition problem in the reaction process, but also can improve the chlorination reaction efficiency, the selectivity and the yield of dichloropropanol, and the purity of the product.

The present application relates to the technical field of biotechnology, and particularly relates to a tagatose-6-phosphate phosphatase mutant and a method for synthesizing tagatose from scratch. The method provided by the present application is a one-pot method for biosynthesizing D-tagatose by using starch or dextrin as a substrate, adopting a multi-enzyme cascade reaction system composed of a tagatose-6-phosphate phosphatase mutant, a glycogen-debranching enzyme, an alpha-glucan phosphorylase, a phosphoglucomutase, a glucose-6-phosphate isomerase, a D-tagatose-6-phosphate-3-epimerase and a 4-alpha-glucan transferase. The method can prepare tagatose from ordinary and inexpensive starting materials (starch or dextrin) at a high yield, improves the overall conversion rate of the system and the final yield of tagatose, provides a new technical approach for realizing low-cost and green production of tagatose, and has the advantages of high yield, low cost and being more suitable for industrial production.

The present application relates to the field of electroenzymatic organic synthesis, in particular to a rhodium-based catalyst and a preparation method and use thereof, the rhodium-based catalyst has the method of electrocatalytic reduction of CO2 to formic acid and the use thereof in the coupling multi-enzyme cascade reaction for producing high-value-added chemicals from CO2. The present application provides a rhodium-based catalyst ([Cp*Rh(bpy)H2O] 2+ ) and a method for simultaneously electrocatalytically reducing CO2 to formic acid and reducing oxidized coenzyme I (NAD + ) to obtain reduced coenzyme I (NADH). The above-mentioned rhodium-based catalyst effectively solves the limitation of low activity and low efficiency of enzymatic reduction of carbon dioxide to formic acid, and at the same time lays the foundation for providing necessary reducing power (NADH) for subsequent multi-enzyme cascade, and finally realizes high-added chemicals directly from CO2.

The invention discloses a hydrogen esterification reaction method taking carbon dioxide as a carbonyl source, and belongs to the field of organic synthesis of application of an electro-catalysis technology. A copper-zinc electrode is applied to carbon dioxide electroreduction, an acetonitrile solution with 1-butyl-3-methylimidazolium hexafluorophosphate as supporting electrolyte serves as a carbon dioxide electroreduction solution, dilute sulphuric acid provides a proton source for the carbon dioxide electroreduction solution, carbon dioxide is efficiently and selectively reduced into carbon monoxide mixed gas, the carbon monoxide mixed gas is subjected to a cascade reaction process, and the carbon dioxide is obtained. Removing redundant carbon dioxide from the generated mixed gas through a saturated KOH aqueous solution; and then directly introducing the mixture into an unsaturated hydrocarbon reactor, and directly synthesizing an esterification product in one step under the catalytic action of metal palladium and a phosphine ligand.

The invention belongs to the technical field of genetic engineering, and discloses an inositol-3-phosphate synthase mutant and a preparation method of inositol. Compared with an amino acid fragment as shown in SEQ ID NO: 1, the inositol-3-phosphate synthase mutant provided by the invention comprises substitution mutation at one or more sites of the 132 site, the 181 site, the 211 site and the 324 site, and the introduction of the substitution mutation can realize structural optimization of a catalytic active center in the inositol-3-phosphate synthase; the method has the advantages that substrate combination, oxidation reaction and condensation reaction are benefited, so that the obtained inositol-3-phosphate synthase mutant is endowed with excellent activity of catalyzing glucose-6-phosphate to generate inositol-3-phosphate and can be well applied to in-vitro enzyme cascade reaction for synthesis of inositol, high-yield and high-efficiency production of inositol is realized, and the method is suitable for industrial production. And the method has an excellent practical application prospect.

The invention discloses hydrophilic super-macroporous polystyrene (HGPS (at) MAF-7 (at) FDH) composite microspheres loaded with an inorganic / organic hybrid cascade catalytic system and a preparation method of the hydrophilic super-macroporous polystyrene (HGPS (at) MAF-7 (at) FDH) composite microspheres. The method comprises the following steps: coupling HGPS microspheres with an organic ligand triazole through surface epoxy modification, and reacting the HGPS microspheres with a metal ion solution to obtain a metal ion-loaded microsphere skeleton; then dispersing the framework material in a buffer solution, adding an enzyme solution and an organic ligand triazole, and carrying out a one-pot reaction to obtain the HGPS-coated MAF-7-coated FDH composite microsphere which is used as an inorganic / organic hybrid cascade catalytic system to catalyze CO2 conversion to generate formic acid. According to the method, the defects that a nano material is easy to aggregate and collapse and difficult to recover are effectively overcome, and the material has high specific surface area, excellent mass transfer performance, biocompatibility and mechanical stability. The characteristics of the MAF-7 organic ligand and the controllable porous structure are beneficial to adsorption of CO2 gas, and the MAF-7 not only can be used as a nano-enzyme for replacing carbonic anhydrase to catalyze a CO2 hydration reaction, but also can be used as a carrier for immobilizing formate dehydrogenase. According to the HGPS and MAF-7 and FDH composite microspheres, the complexity of a multi-enzyme cascade reaction is simplified, the stability and robustness of enzymes are improved, a substrate channel is shortened, the overall reaction efficiency is improved, and the HGPS and MAF-7 and FDH composite microspheres have excellent popularization prospects in the fields of CO2 bioconversion and wider cascade reaction system biocatalysis.

The invention discloses a preparation method of all-trans-farnesol through a three-enzyme cascade reaction. The invention firstly provides a mutant of farnesyl pyrophosphate synthase, and the amino acid sequence of the mutant is shown as SEQ ID No.3. Compared with a wild type, the mutant is applied to enzyme catalytic synthesis of farnesol, so that the conversion rate of a gold product is remarkably improved. The invention also provides a three-enzyme cascade reaction preparation method of farnesol, which comprises the following steps: in the same reaction system, dimethyl allyl diphosphate is used as a substrate; according to the method, isopentenyl diphosphate isomerase, farnesyl pyrophosphate synthase or a mutant thereof and farnesyl pyrophosphatase are used as catalytic enzymes for enzymatic reaction to prepare all-trans-farnesyl alcohol. According to the invention, in a single reaction system, three key enzymes are combined for application, so that continuous catalysis, high-efficiency and high-selectivity preparation of all-trans-farnesol is realized, process steps are simplified, and a new way is provided for green and efficient preparation of farnesol.

The invention relates to an electric pulse mediated electrochemical carbon capture type nitrogen-rich copper-based catalyst as well as a preparation method and application thereof. The catalyst is of a Cu / Cu2O-coated NCS core-shell structure, a core phase is a Cu / Cu2O composite phase, and a shell layer is a nitrogen-rich carbon layer rich in sp2-hybrid pyridine nitrogen sites. Through a Stber method, roasting doping and load activation step-by-step preparation, a nitrogen-free doping control sample is synchronously prepared to verify performance advantages. According to the catalyst, a dynamic response integrated system is constructed through coordination of an ECC function and pulse potential regulation and control, CO2 capture-release and time-space precise conversion are realized, a CO2 and nitrate reduction competition path is reconstructed into an ordered cascade reaction, and C-N coupling is directionally promoted to generate urea. The problems that an existing catalyst is low in urea yield, difficult in reactant regulation and control and high in energy consumption are solved, the urea yield reaches 36249 [mu] g.h <-1 >. Mg <-1 > under the optimized pulse potential, nearly 31% of energy consumption is reduced, near-zero carbon emission can be achieved with industrial nitrate wastewater as a nitrogen source, and a new normal form is provided for green urea electrocatalytic synthesis.

The invention discloses a multifunctional nano-enzyme with self-driven cascade catalytic activity and a preparation method and application thereof, and relates to the field of diabetes wound repair materials, and the nano-enzyme comprises the following components: copper peroxide nano-dots, a zinc-based metal organic framework and glucose oxidase. According to the invention, a step-by-step synthesis strategy is adopted, copper peroxide nano-dots are uniformly encapsulated in a zinc-based metal organic framework, and then glucose oxidase is immobilized through surface modification, so that the nano-enzyme is constructed. The nano enzyme can realize self-driven triple enzyme cascade reaction through ingenious structural design, and plays a synergistic treatment role in a diabetes infected wound microenvironment. The nano-enzyme can effectively reduce blood sugar, efficiently sterilize, relieve inflammation and promote revascularization, and an innovative and efficient treatment scheme is provided for treatment of diabetes infected wounds.