292 results about "Cascade reaction" patented technology

The invention discloses a biosynthesis system for high-yield 1, 6-hexamethylenediamine and application of the biosynthesis system, and belongs to the technical field of biology. According to the method, key genes which are excavated and optimized in a 1, 6-hexamethylenediamine biosynthetic pathway are co-expressed in a single cell module or a multi-cell module, and different enzyme elements expressed by the cell module are subjected to enzyme cascade reaction, so that 1, 6-hexamethylenediamine can be efficiently synthesized by taking caprolactam or 6-aminocaproic acid as a substrate; furthermore, after the carboxylic acid reductase MaCAR is mutated, the catalytic activity of the carboxylic acid reductase MaCAR can be remarkably improved, and the yield of the 1, 6-hexamethylenediamine is further improved; in addition, the biosynthesis system disclosed by the invention directly takes the cell module as a whole-cell catalyst, and an enzyme purification step is avoided, so that the biosynthesis system has the advantage of low cost.

The invention discloses a formate dehydrogenase mutant and application of the formate dehydrogenase mutant in catalysis of carbon dioxide reduction, the 223 arginine (Arg) of an amino acid sequence of formate dehydrogenase PsFDH48 derived from Paracoccus sp. MKU1 is mutated into proline (Pro) or the 242 proline (Pro) is mutated into phenylalanine (Phe) through a site-specific mutagenesis technology, and the obtained mutants R223P and P242F have higher catalytic activity and can be applied to catalysis of carbon dioxide reduction. The catalyst can catalyze CO2 to generate formic acid more effectively in vitro, and has potential application value in the aspects of efficient activation of CO2 and further conversion of CO2 into other mono-carbon compounds through cascade reaction.

The invention relates to the technical field of sewage treatment, and particularly provides a method and a system for realizing beneficiation wastewater N, N-diethyldithiocarbamate-C-N-S synergistic oxidation-reduction coupling treatment by an MABR oxygen layering process. According to the method and the system, a time sequence dissolved oxygen (DO) dynamic regulation and control strategy is innovatively developed: through periodic / pulse type switching anaerobic-hypoxia-aerobic conditions, oxygenolysis and ammonification reaction of N, N '-diethyldithiocarbamate are intensified directionally, and toxicity inhibition of intermediate products on functional bacteria is relieved. Based on the strategy, the system can accurately utilize biological membrane oxygen gradient spatial distribution in a single MABR reactor to realize a cascade reaction of sulfur oxidation-ammonification-aerobic nitrification-anoxic denitrification of ethyl sulfur nitrogen; or aiming at high-concentration diethyldithiocarbamate (gt; 50 mg / L) is coupled with a front anaerobic reactor, and ammoniation detoxification and denitrification nitrogen removal are enhanced.

The invention discloses a novel biosynthesis path for producing 3 '-phosphoadenosine-5'-phosphosulfuric acid and application, and belongs to the technical field of bioengineering. According to the invention, through recombinant expression and purification of ribose kinase, ribose phosphate pyrophosphate kinase, ribose phosphate transferase, adenine thiotransferase, adenosine phosphate sulfate kinase and polyphosphate kinase 2, a six-enzyme cascade reaction taking ribose and adenosine as substrates in vitro is constructed, the molar conversion rate reaches 22.85%, and the escherichia coli and bacillus subtilis PAPS cell factory can be used for preparing the recombinant ribose phosphate pyrophosphate kinase, the ribose phosphate transferase, the adenine thiotransferase, the adenosine phosphate sulfate kinase and the polyphosphate kinase 2. The yield of PAPS in a 5-L fermentation tank reaches 7.60 g / L and 5.03 g / L respectively. The PAPS synthesis route provided by the invention provides an extensible, economical and efficient solution for the production of sulfated compounds.

The invention belongs to the technical field of coke oven gas purification treatment, and particularly discloses a method for removing hydrogen sulfide in coke oven gas through photocatalysis, generation of oxygen vacancies is promoted by adopting a synthesis concept of synergistic effect of silver doping and polyethyleneimine, polyethyleneimine provides an alkaline condition on one hand, lone electron pairs on nitrogen are coordinated with zinc on the other hand, and the hydrogen sulfide in the coke oven gas is removed through photocatalysis. The preparation method comprises the following steps: by taking H2S and polyethyleneimine as raw materials, generating abundant oxygen vacancies under the synergistic effect of the H2S and silver, preventing agglomeration of generated ZnO and increasing reaction sites by taking polyethyleneimine as a surfactant, and then promoting a cascade reaction between ZnO < 1-x > and H2S by taking abundant oxygen vacancies, so that a 1D / 3D-ZnS / ZnO heterojunction is generated in situ to further reduce recombination of photo-generated electron holes; the catalyst converts H2S into elemental sulfur through photo-induced electrons and holes, and a carbon disulfide solution is used for cleaning the catalyst to realize regeneration of the catalyst. The method provided by the invention not only improves the removal efficiency of H2S, but also reduces the operation cost and reduces environmental pollution.

The application discloses a formic acid dehydrogenase mutant and application thereof in catalyzing carbon dioxide reduction. Paracoccus The formic acid dehydrogenase from sp.MKU1 is mutated by a site-directed mutation technology Ps The arginine (Arg) at the 223th position of the FDH48 amino acid sequence is mutated into proline (Pro) or the proline (Pro) at the 242th position is mutated into phenylalanine (Phe), and the obtained mutants R223P and P242F have higher catalytic activity, can more effectively catalyze CO2 to generate formic acid in vitro, and have potential application values in efficient activation of CO2 and further conversion into other one-carbon compounds through cascade reactions.

The invention discloses a method for preparing high-value chemicals through photocatalytic driving of beta-O-4 lignin depolymerization and coupling cascade reaction, and belongs to the technical field of photocatalytic biomass resource conversion. The method comprises the following steps: (1) dispersing a beta-O-4 lignin raw material into a solvent containing a photocatalyst; (2) introducing a normal-temperature chemically stable gas to replace air in a reaction system; and (3) carrying out a depolymerization reaction of beta-O-4 bonds and an in-situ C-C bond coupling reaction under an illumination condition to generate a target product. And (4) separating and purifying the product and recovering the catalyst. Through photocatalytic cascade reaction design, the defects of harsh reaction conditions and multi-step separation in a traditional method are avoided, the method has the advantages of mild reaction conditions, high atom utilization rate, high product selectivity and environmental friendliness, and a new way is provided for high-value utilization of lignin.

The invention discloses 5-hydroxymethylfurfural and a preparation method thereof, and belongs to the technical field of chemical engineering. The preparation method comprises the following steps: reacting a homogeneous aqueous solution containing fructose, inorganic salt and an acid catalyst in a multi-kettle series reactor to obtain the 5-hydroxymethylfurfural, the inorganic salt is selected from at least one of halogen compounds, borides, silicate, aluminate, phosphate and borate; the acidic catalyst comprises at least one of water-soluble acids. The specific inorganic salt and the specific acid catalyst are added and can play a synergistic role, the reaction is carried out under multi-kettle series connection, the backmixing degree of the reaction materials in the reactor is maximized, side reactions can be effectively controlled, black rot is avoided, the reaction production efficiency and conversion rate are effectively improved, the reaction selectivity is improved, and the method is suitable for industrial production. And the equipment maintenance and labor cost is greatly reduced.

The application discloses an emulsion microreactor for cascade reaction and a preparation method and application thereof. The emulsion microreactor is prepared by the following steps: acidolysis of agar, hydrophobic modification, mixing of obtained interface stable modified agar nanoparticles, enzymes and buffer to obtain an aqueous phase; mixing of a substrate, a catalyst and an organic solvent to obtain an oil phase; and mixing of the aqueous phase and the oil phase to obtain the emulsion microreactor. The emulsion microreactor is used for synthesizing 4-biphenylcarboxylic acid ethyl ester by using lipase and a metal palladium catalyst, solves the problem of poor compatibility between the catalyst metal palladium and the lipase, spatially separates the lipase and the metal palladium, improves the cascade catalytic efficiency of the metal enzyme, and finally the yield of the product 4-biphenylcarboxylic acid ethyl ester can reach 55.85%; and the metal enzyme cascade reaction is completed in one pot in the emulsion microreactor, avoids separation of an intermediate product, expands the range of catalytic reactions, and provides a reaction platform for other high-value-added pharmaceutical intermediate products.

The invention discloses a method for efficiently producing pyridoxal phosphate based on a three-enzyme cascade reaction and a recombinant strain, and belongs to the technical field of biological engineering. The recombinant escherichia coli for co-expressing the pyridoxine phosphate phosphatase mutant, the pyridoxine phosphate oxidase and the hydrogen peroxide decomposing enzyme is constructed, three-enzyme synergy is realized by optimizing the copy number of a carrier, and the catalytic efficiency of a recombinant strain as a cell catalyst is improved. The invention further provides a one-pot method for preparing pyridoxal phosphate, the production cost is reduced, an efficient, green and economical biosynthesis path is provided for industrial production of PLP, and the method can be widely applied to the fields of food, medicine and the like and has important industrial value and application prospects.

This invention discloses a method for synthesizing Rotundine and its derivatives, as well as the construction of a whole-cell catalytic system. This invention utilizes an artificially designed multi-enzyme cascade reaction method to efficiently synthesize Rotundine and its derivatives. Furthermore, this invention constructs the enzymes of the synthetic route into E. coli chassis cells to obtain a whole-cell catalyst comprising M1a, M2a, M3a, and M4a. Using the whole-cell catalyst to synthesize Rotundine and its derivatives, the yield of Rotundine can reach 2.44 g / L.

The invention discloses a binary single-site material for controlling spatial distribution of active sites as well as a preparation method and application of the binary single-site material. The binary single-site material comprises SiO2 nanospheres, single-site metal encapsulated in the SiO2 nanospheres and single-site metal anchored on the surfaces of the SiO2 nanospheres, wherein the single-site metal includes at least one of Cu, Zr, Yb, Hf, Y, and Zn. The preparation method adopting a reverse microemulsion method has universality, a high-dispersion single-site catalyst of corresponding metal can be synthesized by changing the variety of coated organic metal salt, the problem that high-dispersion single-metal sites cannot be synthesized in a SiO2 bulk phase is solved, spatial distribution of active sites can be accurately controlled, and the preparation method is suitable for large-scale industrial production. And the catalyst inhibits the generation of a by-product ethylene, significantly improves the catalytic activity and butene selectivity through an ordered catalytic cascade reaction, and can better meet the field of ethanol-to-olefin catalysis.

A fluidized bed device for coupling naphtha and methanol to prepare aromatics and co-produce olefins and its application method are provided. By using the device, under the action of a catalyst, naphtha reacts with methanol to generate product gas containing aromatics and light olefins as main components. The method can efficiently and selectively convert linear and branched aliphatic hydrocarbons into aromatics, while also increasing p-xylene production through aromatic methylation reactions, with the p-xylene content in the xylene mixture exceeding 75 wt %. The fluidized bed reactor achieves increased p-xylene production by controlling the progression of cascade reactions (naphtha→benzene / toluene→p-xylene). Additionally, it utilizes the methylation reaction of benzene / toluene with methanol to provide in-situ heat for the coupled naphtha-methanol aromatization process, thereby achieving autothermal balance.

This invention discloses a method for preparing sulfonamide-modified benzo[a]fluorene-5-one derivatives, using 1,7-enyne and N Using sulfonylaminopyridine salts as raw materials, a series of sulfonated benzo[a]fluorene-5-one compounds were successfully synthesized by catalyzing the sulfonation / cyclization tandem reaction of 1,7-enynes under photocatalytic conditions. This method has excellent functional group tolerance and high economic applicability.

The present application relates to the technical field of organic synthesis, and particularly relates to a method for preparing (+)-crispine A by means of biological enzyme resolution. First, 6,7-dimethoxy-3,4-dihydroisoquinoline-2-oxide is subjected to 1,3-dipolar cycloaddition with allyl alcohol to obtain a first compound; then the first compound is added into a sulfonylation reagent and zinc powder to obtain a racemate second compound through a three-step continuous series reaction of primary alcohol sulfonylation, nitrogen-oxygen bond cleavage and ring formation; then the second compound is mixed with vinyl acetate and biological enzyme, and chiral resolution is carried out under the action of the biological enzyme to obtain a third compound with right-handed optical rotation and a fourth compound with left-handed optical rotation; finally, the third compound is mixed with an alkaline reagent and a sulfonylation reagent, and then secondary alcohol sulfonylation is carried out to obtain a fifth compound with right-handed optical rotation; the fifth compound is mixed with a deoxidizing reagent to carry out deoxidation reaction, and (+)-crispine A is prepared. The method is simple, green and environmentally friendly, and can be produced in large quantities.

The present invention belongs to the field of catalyst technology, and specifically discloses an organic hydrogen transfer aid for propane dehydrogenation and a synthesis method thereof. The preparation method described in the present invention is to first coat the surface of a quinone material (L) with a layer of SiO2, and then perform a hydrothermal crystallization treatment to form an S-1 molecular sieve shell to obtain an L@S-1 material. Through the confinement of the molecular sieve pores, the quinone material can realize a hydrogenation-dehydrogenation cycle at a higher reaction temperature. When the material is used in combination with a noble metal propane dehydrogenation catalyst, a series reaction of propane dehydrogenation and hydrogenation of the quinone material is realized, the hydrogen partial pressure of the propane dehydrogenation process is reduced, and the thermodynamic equilibrium limit is broken through, thereby realizing a propane dehydrogenation reaction at a lower reaction temperature. The reduction in reaction temperature will bring about advantages such as reduced energy consumption and increased catalyst stability.

The invention develops a cascade reaction strategy for realizing efficient synthesis of a caged ketal skeleton compound by taking Bronsted acid as a catalyst and taking a 3-hexene-2, 5-diketone derivative and a cinnamyl alcohol analogue as substrates, and synthesizes a natural product (+ / -)-catunaregin and a series of novel analogues of the natural product (+ / -)-catunaregin which are not reported in literatures. Important technical support and early-stage theoretical basis are provided for the creation of new drugs of the compounds. The cinnamyl alcohol analogue disclosed by the invention is simple and easy to prepare, wide in derivation range and low in price, and can better enrich the structural diversity of caged ketal skeleton compounds. The method disclosed by the invention can be prepared by a one-pot method, is efficient and rapid, simple to operate, short in reaction step, good in substrate tolerance, mild in reaction condition and wide in application range, and overcomes the defects of complex operation, harsh reaction conditions, substrate limitation, intolerant substrate functional groups, tedious steps and the like in the traditional reaction.

The application provides a synthesis method of 2,3-disubstituted benzofuran compounds and application thereof. The application takes o-hydroxy chalcone and gamma-bromobutyric acid ester as reaction raw materials, and obtains a series of 2,3-disubstituted benzofuran compounds through one-pot multi-step tandem reaction under the promotion of alkali. Advantages of the application include: the reaction reagent is cheap and easy to obtain, the reaction is efficient, the reaction step is short, the application is economical and practical, and the application is environment-friendly; the reaction is easy to enlarge, and has practical value. The compounds have certain inhibitory activity on alpha-glucosidase, and can be used for treating diabetes.

The present invention relates to a preparation method and application of a surface protein imprinted polymer relying on an enzyme cascade reaction; the surface imprinted polymer is prepared under conditions of high monomer concentration by mediating local free radical polymerization through an enzyme cascade reaction. The local free radical polymerization reaction is initiated by an initiation system consisting of GOx / HRP, acetylacetone and glucose fixed on the surface of a nanocarrier, so that the polymerization reaction proceeds around the surface of the nanocarrier. The random freedom of free radicals in the entire solution is avoided, and even at high monomer concentrations, no agglomeration phenomenon occurs during polymerization. At the same time, the method is carried out under conditions of high monomer concentration, which is conducive to the sufficient pre-assembly of functional monomers and template proteins, thereby improving the imprinting efficiency. The imprinting capacity of the synthesized nanoimprinted microspheres for lysozyme is 145.3 mg g ‑1 , the imprinting factor is 14.2.

Disclosed in the present invention are a cascade reaction device and method for preparing aviation fuel by means of photoelectrocatalytic conversion of lignin. The method comprises: placing lignin in a pretreatment reaction chamber filled with an alkaline solution, so that the lignin is fully dissolved; opening valves to enable a pretreated reaction liquid to flow into an anode chamber in a photoelectrocatalytic reaction chamber; using solar energy to drive a photoelectrocatalytic reaction at an anode; after sufficient reaction, enabling the reaction liquid in the anode chamber to flow into a temporary storage chamber, and subsequently, using a pump to transfer the reaction liquid in the temporary storage chamber into a cathode chamber; and after sufficient reaction, introducing the reaction liquid in the cathode chamber into a high-pressure reaction chamber by means of a pump, and enabling hydrogen generated in the cathode chamber to flow into a gas cylinder by means of a pipe for collection, so as to be supplied to the high-pressure reaction chamber, wherein the reaction liquid undergoes a hydrodeoxygenation reaction under the action of a catalyst in the high-pressure reaction chamber, and after sufficient reaction, the aviation fuel can be obtained. The present invention achieves an integrated cascade reaction for solar-driven conversion of lignin into aviation fuel.

The invention relates to a process device and method for preparing a carbon nano tube based on serial connection of methane dry reforming and Fischer-Tropsch synthesis reaction, and belongs to the technical field of carbon nano material preparation and chemical processes. The invention provides a process device and method for preparing a carbon nanotube based on serial connection of methane dry reforming and Fischer-Tropsch synthesis reaction, a thermal-thermal serial connection strategy is adopted, and a dual-catalyst-dual-bed coupling serial connection system of a fixed bed (DRM reaction unit) and a tubular furnace (FTS reaction unit) is used for preparing the carbon nanotube. The invention provides a process device for preparing a carbon nanotube based on serial connection of methane dry reforming and Fischer-Tropsch synthesis reaction. The process device mainly comprises a serial connection reaction group and an online detection unit which are connected in sequence.

The application discloses a five-membered ring containing alkynyl or triazole functional group P The chiral phosphine oxide compound is obtained through Domino Heck-Sonogashira cascade reaction by taking a prochiral tertiary phosphine oxide compound containing a double ethylene group and phenylacetylene as raw materials, transition metal palladium catalyst and chiral TADDOL phosphoramidite ligand under the protection of inert gas P The chiral phosphine oxide compound has a product yield of 96% and an enantioselectivity ee value of 99%, and a diastereoselectivity dr value of greater than 20:1. The application adopts an asymmetric desymmetrization strategy, uses a Heck reaction to start and a Sonogashira reaction to end a cascade reaction, simultaneously constructs P The chiral and quaternary carbon chiral center has mild reaction conditions, simple operation and good substrate universality, provides a compound skeleton with diversity, and is successfully applied to a palladium-catalyzed asymmetric allyl substitution reaction as a chiral ligand.

The invention provides a synthesis method and application of a tetra-substituted pyran compound. The tetra-substituted pyran compound is directly constructed through a one-pot cascade reaction under an organic base catalysis system by taking triketone olefin and allyl ammonium salt as key reaction raw materials. The method has the advantages that the operation is simple and convenient, no water or oxygen is needed, the reaction is efficient, the steps are simple, the method is environment-friendly, the reaction is easy to amplify, and the method has practical value. Activity tests prove that the compounds have certain inhibitory activity on alpha-glucosidase, or can be used for treating diabetes mellitus.

This application discloses a method for synthesizing chiral cyano-containing full-carbon quaternary cyclic compounds, characterized by the following steps: mixing a solvent, an oxidant, an alkynyl malononitrile compound, a carboxylic acid compound, a palladium salt, and a chiral dinitrogen ligand; reacting the mixture; drying; and separating to obtain the chiral cyano-containing full-carbon quaternary cyclic compound. This method can achieve a palladium-catalyzed tandem reaction of alkynyl malononitrile with a carboxylic acid involving asymmetric acyl oxidative cyclization / acyl migration, yielding cyano-containing full-carbon quaternary cyclic compounds with optical purity in good yields (enantiomer ratios up to 96.5:3.5). This invention features a broad substrate range, readily available raw materials, simple and practical operation, and mild reaction conditions.

This invention provides a method for preparing 4,1-disubstituted pyrrolo[1,2-a]quinoxaline-1-yl ketone derivatives, belonging to the field of organic synthesis technology. This invention uses 2-(1H-pyrrolo-1-yl)aniline compounds and α-carbonyl acids as reactants, employing only ammonium persulfate as an oxidant. Under metal-free catalysis, a one-pot tandem reaction is carried out to simultaneously complete the cyclization of the pyrrolo[1,2-a]quinoxaline nucleus and the C-1 acylation modification. The substituents at the 4- and 1-positions of the product can be flexibly controlled by changing the α-carbonyl acid with different structures. This method requires no pre-modified substrate, eliminates the need for metal catalysts, avoids the risk of metal residue, and features simplified steps and high functional group compatibility, providing a new green and low-cost route for the synthesis of polysubstituted pyrrolo[1,2-a]quinoxaline ketone derivatives.

The invention provides a metal-heteropolyacid catalyst confined to a molecular sieve as well as a preparation method and hydrodeoxygenation application of the metal-heteropolyacid catalyst. The catalyst takes a spherical pure silicon molecular sieve with the particle size of 2-3mm as a carrier, and transition metal and heteropoly acid active components are supported in a pore channel in a limited range; the mass ratio of the heteropoly acid to the metal is (10-50): 1. According to the invention, an organic amine bridging strategy is adopted to construct near double active sites, that is, organic amine molecules in heteropolyacid organic amine salt are used as a guiding anchoring agent, and a transition metal precursor is accurately anchored near heteropolyacid. After roasting, a concerted catalysis center in which a metal hydrogenation site and a heteropolyacid deoxidation site are closely close on the nanoscale is successfully constructed in a confinement space of the molecular sieve, and the mass transfer efficiency and the step coupling effect in a hydrodeoxygenation multi-step cascade reaction are improved. When the weight hourly space velocity is 2 g / gcat. / h, the temperature is 150-250 DEG C and the pressure is 0.5-2 MPa, the conversion rate of the oxygen-enriched phenolic compound can be greater than or equal to 94%, and the yield of cycloalkane can be greater than or equal to 90%.

This invention discloses an α-indolylpyrrolo[1,2-a]indole derivative and its preparation method. The derivative is a levorotatory or dextrorotatory optically active form or racemate with the following structural formula: The preparation involves using α-indolylpropynyl alcohols and indole compounds as raw materials, and 3,5-bis(trifluoromethyl)phenylbinaphthylphosphonic acid as a catalyst, reacting in an organic solvent to obtain the α-indolylpyrrolo[1,2-a]indole derivative. This invention utilizes an organophosphate-catalyzed tandem reaction of two components to synthesize the α-indolylpyrrolo[1,2-a]indole derivative. The reaction conditions are mild, the process is simple, and the operation is convenient. The obtained product is the core skeleton of the indole bases of the natural products isoborreverine and dimethyl isoborreverine, which is of great significance for the synthesis of analogs of these natural products.

The application is suitable for the field of analog enzyme technology, and provides a light-controlled zein-based dual-enzyme-activity analog enzyme and a preparation method thereof.The preparation method comprises the following steps: under water bath conditions, dissolving zein in an alkaline aqueous solution and incubating; adding a hydroxylamine solution to the solution obtained in step S1 and continuing to react; and after the reaction is completed, obtaining the light-controlled zein-based dual-enzyme-activity analog enzyme.The application uses zein as a raw material, is modified by hydroxylamine, does not need any metal component to participate, has light-controlled switchable dual-enzyme activity, exhibits oxidase-like activity under visible light irradiation, presents peroxidase-like activity in dark conditions, and can realize reversible switching of the two enzyme activities by regulating light, effectively overcomes the problems that traditional artificial analog enzymes depend on metal ions, multiple enzyme activities coexist, interfere with each other and are difficult to accurately control, can be used for colorimetric detection of total antioxidant capacity, can be used for colorimetric detection of glucose in cascade reaction with glucose oxidase, has the advantages of simple operation, rapid response, high sensitivity, good selectivity, low cost and the like, and has a good application prospect in the fields of food analysis, clinical diagnosis and the like.

The invention discloses a method for synthesizing mesalazine and application of mesalazine. The method comprises the following steps: introducing p-nitrophenol, formic acid and a Cu-Co-coated MFI-NS molecular sieve catalyst into a continuous flow reaction zone, and sequentially carrying out electrophilic substitution and nitro hydrogenation cascade reaction at normal pressure and 50-70 DEG C; the catalyst has a micropore-mesopore stepped pore channel, and contains a Cu0 metal cluster and a framework Co < 2 + > acid site. And separating, acidifying and crystallizing the reaction liquid to obtain a finished product. According to the invention, the shape-selective selectivity and difunctional synergistic effect of the molecular sieve are utilized, high conversion rate (gt, 98%) and high selectivity (gt, 94%) under mild conditions are realized, the product purity is greater than or equal to 99.5%, and the Cu / Co residue meets the ICHQ3D standard. The catalyst can be recycled for 10 times or more, the yield is reduced by 5% or less, iron mud pollution of a traditional iron powder method and the metal leaching risk of a noble metal catalyst are thoroughly eliminated, and the method has the remarkable advantages of being environmentally friendly, efficient, low in cost, easy to industrially amplify and the like and is suitable for preparing the medicine for treating the inflammatory bowel disease.

This application discloses a multi-enzyme complex based on a Snap-Fit self-assembly tag, its construction method, and its applications, relating to the field of protein engineering technology. The multi-enzyme complex based on the Snap-Fit self-assembly tag comprises: a TLL-FitC fusion protein with the amino acid sequence shown in SEQ ID NO.13, a FitN-AnChOx fusion protein with the amino acid sequence shown in SEQ ID NO.14, and an OleT protein with the amino acid sequence shown in SEQ ID NO.15. PRN - SnapG fusion protein. This multi-enzyme complex employs a Snap-Fit assembly strategy, which allows three different enzymes to be assembled using only one set of tags. When applied to the synthesis of olefins, it helps to improve the transfer efficiency of 1-olefin cascade reaction intermediates between upstream and downstream enzyme active sites, thereby increasing the final yield, and has great application potential.