The present invention relates to a kind of β-1,4-
glucanase and application thereof, belong to the field of
biotechnology. The present invention screens and identifies a kind of novel β-1,4-
glucanase in tiger milk
mushroom, the β-1,4-
glucanase of the present invention has wide
substrate specificity, can specifically hydrolyze the β-
glucan (such as
sodium carboxymethyl cellulose) containing β-1,4 glycosidic bonds, can hydrolyze the β-
glucan (such as barley β-
glucan and oat β-glucan) containing β-1,3-1,4 glycosidic bonds again, therefore the β-1,4-glucanase of the present invention has good
enzyme activity to the β-glucan containing β-1,4 glycosidic bonds and the β-glucan containing β-1,3-1,4 glycosidic bonds. In addition, the β-1,4-glucanase of the present invention is under the conditions of pH 4.0-7.0 and its
enzyme activity can keep more than 70%, and at 40 DEG C, its
enzyme activity can keep more than 88%, with good stability.