The application provides a preparation method and application and composition of oat beta-
glucan, and relates to the technical field of
cosmetics.The preparation method comprises the following steps: (1) oat is crushed, mixed with
alkaline water, and subjected to subcritical
water extraction at 150-180 DEG C to obtain a crude extract; (2) the pH of the crude extract is adjusted to be acidic, the crude extract is allowed to stand, and the supernatant is taken; (3) the pH of the supernatant is adjusted to be neutral, then
amylase and
neutral protease are added to perform enzymatic reaction, and the filtrate is obtained through
filtration; and (4) the filtrate is purified through Sephacryl S-200 gel resin, concentrated, and oat beta-
glucan is obtained.The method significantly improves the yield of oat beta-
glucan, maximally retains the anti-aging activity of oat beta-glucan, and has better anti-aging, anti-
wrinkle and
skin elasticity improvement effects; oat beta-glucan and other anti-aging ingredients are mutually matched, and the effect of significantly removing
DPPH free radicals is brought.