115 results about "Beta d glucan" patented technology

The invention discloses a radiation-resistant and moisture-retention sun-blocking skin-care product, which contains 2-5 wt% of oat beta-glucan. The radiation-resistant and moisture-retention sun-blocking skin-care product provided by the invention can be used to raise radiation resistance and immunity of the whole skin and construct healthy skin, has an anti-allergic effect, can effectively prevent / alleviate various inflammation and symptoms such as erythema, and can preserve moisture, minimize fine lines and smoothen skin.

The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability, including thermotolerance or thermostability, at increased or decreased pHs and temperatures.

The invention discloses a hyaluronic acid dressing and a preparation method thereof. The dressing comprises the following components in percentage by mass: 0.01-0.3% of medium-molecular-weight hyaluronic acid, 0.01-0.5% of small-molecular-weight hyaluronic acid, 0.03-1% of allantoin, 0.1-1% of Carbomer, 2-5% of sorbitol, 0.2-1% of trehalose, 0.7-2.5% of witch hazel distillate, 0.1-0.5% of D-panthenol, 0.05-0.15% of alpha-bisabolol, 0.2-1.0% of oat glucan, 2-10% of glycerinum, 0.06-1% of water-soluble silicon wax, 0.05-0.9% of an antalkali, 0.004-0.2% of 2-methyl-4-isothiazoline-3-one, 0.05-0.4% of phenoxyethanol, 0.005-0.05% of pentanediol, 0.01-0.06% of octylene glycol, 0.025-0.5% of methylparaben and the balance of water. The dressing disclosed by the invention is capable of permeating into the deep layers of the skin to remove excessive free radicals, promoting the generations of blood vessels, and accelerating the union of wounds, in particular, acnes, eczematous dermatitis, photorejuvenation postoperation repair and the like.

Devices to detect detecting endotoxin (lipopolysaccharide or LPS) and / or 1-3-β-D-glucan (beta glucan or BG) using substrates including electrogenic mediators for amperometric detection are provided herein. Substrates include an amine group as well as one or more substituted organic rings, such as a phenol. Devices include cartridges for detecting LPS or BG simultaneously using dual detection techniques.

The invention provides moisturizing mask liquid and a preparation method thereof. The moisturizing mask liquid comprises water, glycerol, propylene glycol, polyglycerol-10, hydroxyethylurea, snail liquid, deep-sea salmon collagen, gynostemma pentaphylla extracts, grass green salicornia herbacea extracts, natto fermentation essence, oat beta-glucan, carboxgpolmethylene, triethanolamine, sodium hyaluronate, methylparaben, phenoxyethanol and essence. The moisturizing mask liquid has the advantages that the moisturizing mask liquid is high in moisturizing effect and rich in nutrition and can be easily absorbed by the skin of a user, the skin of the user can be sufficiently moisturized, the skin of the user is allowed to be moist, tender and glossy, and the skin of the user can be effectively kept moist all day long.

The invention provides a preparation method of allergen-free oat beta-glucan. The preparation method comprises the following steps: (1) microwave drying; (2) carbon dioxide supercritical extraction; (3) amylase hydrolysis; (4) glucoamylase hydrolysis; (5) alkali extraction; (6) isoelectric point protein precipitation; (7) centrifugal separation to collect the supernate; (8) protease hydrolysis; (9) flocculant precipitation; (10) centrifugal separation to collect the supernate; (11) membrane separation; (12) vacuum condensation; (13) alcohol precipitation; (14) centrifugal separation to collect the precipitate; (15) vacuum drying. The oat beta-glucan extracted from oat bran does not contain any allergen, has the advantage of high purity, and has a specific molecular weight.

The invention discloses a method for extracting high-purity beta-glucosan from oat bran. According to the method, oat bran powder is sequentially treated by adopting the following steps: (1) carrying out ethyl alcohol reflux and enzyme deactivation; (2) stirring and extracting in hot water; (3) centrifuging and collecting supernatant; (4) carrying out enzymolysis and isoelectric precipitation; (5) carrying out alcohol precipitation; (6) centrifuging, collecting and precipitating; (7) carrying out ammonium sulfate precipitation; (8) dialyzing and removing impurities; and (9) carrying out freeze drying, so that the oat beta-glucosan is obtained. The high-purity oat beta-glucosan is obtained by adopting a mild water extraction method through extraction and treatment steps such as enzymolysis, isoelectric precipitation, alcohol precipitation, ammonium sulfate precipitation and dialysis. The high-purity oat beta-glucosan has a certain antioxidation effect on oil and can be added to yoghourt so as to improve the texture characteristics and nutritional ingredients of the yoghourt.

The invention provides a method for extracting beta-D-glucan from cell walls of candida utilis. The method includes steps of acquiring cells of the candida utilis; carrying out induction autolysis on the cells of the candida utilis; carrying out high-temperature digestion; ultrasonically breaking the walls; carrying out high-temperature extraction; carrying out degreasing; carrying out enzymatic hydrolysis to remove proteins, to be more specific, preparing suspension from degreased crude glucan by the aid of phosphoric acid buffer solution, carrying out enzymatic hydrolysis, carrying out reaction, then washing reaction products by the aid of water, centrifuging the reaction products, adding water into the reaction products, sufficiently uniformly mixing the reaction products and the water with one another to obtain mixtures, then heating the mixtures, deactivating enzymes, centrifuging the mixtures and freezing and drying the mixtures to obtain beta-D-glucan products. The method has the advantages that product degradation effects due to the traditional methods implemented by the aid of acid and alkali reagents can be prevented, and the method for extracting beta-glucan can be carried out under mild conditions; potato juice without the proteins is used as a culture medium for the candida utilis, accordingly, environmental pollution due to the discarded potato juice can be abated, and the method has important significance on environmental protection.

The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability, including thermotolerance or thermostability, at increased or decreased pHs and temperatures.

The invention provides a kit for detecting a fungus (1-3)-beta-D glucan in human body fluid. The kit can be industrially produced, and is accurate and high in speed and specificity. The kit is characterized by comprising a main reactant, a body fluid treating agent, an alkaline diluent, an acidic diluent, sterile water, lyophilized serum and a (1-3)-beta-D glucan quality control substance, wherein the main reactant contains a tachypleus tridentatus blood cell G factor, coagulating zymogen and an activator. A detection method is convenient, rapid, safe and high in specificity, the (1-3)-beta-D glucan in body fluid samples of 10 persons can be detected within 80 minutes, a discrimination coincidence rate reaches 100 percent, and the fungus (1-3)-beta-D glucan and bacterial endotoxin can be discriminated within the same reaction time.

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle toner and a preparation method thereof. The anti-wrinkle toner consists of water, glycerin, propylene glycol, polyglycerol-10, butanediol, oat Beta-glucan, lycine, hydroxyethylurea, milk protein, lactobacillus / pomegranate fruit fermentation product extract, starfish essence, allantoin, sodium hyaluronate, preservative and essence. The anti-wrinkle toner is mild, skin-friendly, safe and non-irritating, can effectively moisturize, preserve moisture, resist aging, repair wrinkles and ensures that skin is moist, smooth and elastic, and can be used for a long term.

The invention discloses a polysaccharide combination, which is prepared by 0.01-10 percent of chitosan, 0.01-20 percent of oat beta-glucan and 70-99.98 percent of water. The polysaccharide combination is prepared through a method which comprises the following steps of: dissolving oat beta-glucan in the water to prepare oat beta-glucan dissolved water solution; and adding water into the chitosan for swelling, removing foams after one night to prepare chitosan gel, and mixing and evenly agitating the two kinds of solutions to finally obtain the polysaccharide combination which is prepared by using 0.01-10 percent of chitosan, 0.01-20 percent of oat beta-glucan and 70-99.98 percent of water. The polysaccharide combination can be used for the preparation of medical dressings, wound care agents, medicines for preventing and curing radiodermatitis, medicines for curing ulcers caused by bedsores and diabetes mellitus and dental ulcers, and medicines for promoting wound healing and reducing scars.

Objects of the present invention are to provide a DNA fragment encoding a limulus-derived pro-clotting enzyme, a virus harboring the DNA fragment, a cell harboring the virus, a method of producing the pro-clotting enzyme by use of the cell, and means for assaying an endotoxin or (1→3)-β-D-glucan employing the enzyme, wherein these elements are capable of producing an endotoxin or (1→3)-β-D-glucan assay reagent of satisfactory quality, steadily, at low cost, and on a large scale. In the present invention, for example, a DNA fragment encoding a protein having an amino acid sequence defined by SEQ ID NO: 4 is selected as a nucleic acid fragment encoding a limulus-derived pro-clotting enzyme, and the corresponding recombinant pro-clotting enzyme. Use of the enzyme can provide a high-sensitivity method and kit for detecting (1→3)-β-D-glucan and an endotoxin, utilizing a cascade reaction system in a horseshoe crab lysate.

The invention belongs to the technical field of special diet foods, and particularly relates to an athletic nutrition food namely whey protein powder having the function of relieving weariness and a preparation method of the whey protein powder. According to the preparation method disclosed by the invention, isomaltitol, haematococcus pluvialis derived astaxanthin powder, trehalose, calcium beta-hydroxy-beta-methylbutyrate, ginseng, oat-beta-glucan, probiotics and medicinal and eatable traditional Chinese medicine mixed powder. The nutrient components are in synergistic reaction to generate technical results beyond all expectation, nutrient components of the whey protein powder can be further increased, and the function of the whey protein powder for relieving weariness can be further improved. The athletic nutrition food namely the whey protein powder having the function of relieving weariness disclosed by the invention is reasonable in formula, comprehensive in nutrients and safe toeat; and animal experiment results display after the athletic food disclosed by the invention is scientifically eaten, physical fitness can be quickly complemented, and weariness can be effectively relieved.

The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.

The invention disclose a preparation method of yeast beta-D-glucan. The method comprises the following steps that (1) a brewer yeast is added to water to form a suspension a; NaC1 is added to the suspension a; induced autolysis, enzymatic inactivation and then centrifugation are conducted; a sediment a is obtained; (2) a phosphate buffer is added to the sediment a to form a suspension b; a sediment b is obtained by extraction and centrifugation; (3) water is added to the sediment b to form a suspension c; the suspension c is subjected to microjet homogenization treatment and then centrifugation to form a sediment c; (4) a phosphate buffer b is added to the sediment c to form a suspension d; enzyme is added to the suspension d for enzymolysis; then enzymatic inactivation treatment is conducted; (5) an enzymatic hydrolysate treated in Step (4) is subjected to the microjet homogenization treatment and then centrifugation to form a sediment d; and (6) the sediment d is dehydrated and dried in sequence to form yeast beta-D-glucan. The method can realize comprehensive utilization of industrial by-products, namely waste beer yeasts and increase the utilization ratio and added values of the industrial by-products, and has significant economic benefits and environmental protection significance.

The invention relates to an oat beta-glucan hypolipemic capsule prepared by oat beta-glucan and flax-seed oil. The invention also relates to a preparing method for the oat beta-glucan hypolipemic capsule, comprising weighing the oat beta-glucan and flax-seed oil in proportion by weight of 5-40:60-95, mixing for 10-30 mins in a mixer, detecting the evenness, filling into the capsules by the capsule filler, obtaining the oral oat beta-glucan hypolipemic capsule. The invention has the efficacy as follows: reducing blood serum total cholesterin, adjusting blood sugar level, losing weight, diminishing hyperalimentation, improving intestine and stomach function; waste discharging and youth keeping, defecating, preventing colon cancer and enhancing immunity.

The invention relates to a developing-process fungus 1,3-beta-D-glucan detection kit for human body fluid. The developing-process fungus 1,3-beta-D-glucan detection kit comprises a reaction main agent, a main agent compound solution, a sample treatment solution, heat-source-free water, a standard product and a quality control product, wherein the reaction main agent takes horseshoe crab blood cells as a main raw material and contains G factors, coagulase, coagulase zymogen and a polypeptide developing substrate; the polypeptide developing substrate is synthesized tripeptide or tetrapeptide with a Gly-Arg tail end connected with a PNA; the polypeptide developing substrate is subjected to enzyme digestion by adopting the coagulase; after the free paranitroaniline (PNA) is generated, a microplate reader is used for directly detecting so that a detection route is shortened and the cost is reduced; the microplate reader is used for carrying out a velocity-method enzyme kinetics detection method so that the sensitivity is relatively high when being compared with a nephelometry detection method; and the reaction main agent is not easily interfered by protein in a body fluid sample and medicines to generate non-specific turbidity, so that the probability of a false positive detection result is reduced and the detection accuracy is relatively high.

The invention discloses functional bread with beta-glucan. The bread is made from wheat flour, beta-glucan, pure water, yeast, sugar, salt and oil which are mixed in a certain ratio, and is made after stirring, mixing, fermentation, shaping, secondary fermentation and baking. beta-glucan is water-soluble fiber with the role of reducing cholesterin, reducing blood glucose and strengthening immune function and an ideal additive of bread production and has higher application value in actual production. The invention has the advantages of soft flavor, being rich in elasticity, being refreshing and not sticking, smell of baking products, the plump shape, the smooth surface, softness and elasticity, the even and fine bread, the swelling volume, the strong preservation ability and extending about 85 percent of shelf service life than that of the ordinary bread.

The invention relates to a chopped birch enzyme and a preparation method thereof. The enzyme is prepared from, by weight, 55-65 parts of enzyme extractives, 0.05-0.15 part of composite lactic acid bacterium powder, 0.3-0.7 part of yeast powder, 30-40 parts of erythritol and 3-7 parts of arabinose. The chopped birch enzyme contains abundant polysaccharide substances, beta-D-glucan has the high SODactivity, the active effect can be achieved for promoting insulin secretion, the prepared chopped birch enzyme contains various abundant flavone substances, the good blood sugar reduction effect is achieved, and the chopped birch enzyme can be adopted as auxiliary products for preventing and treating high blood sugar; chopped birch, corn stigma, chrysanthemum flowers, Coptis chinensis and burdockare fermented by probiotics, flavone substances can be sufficiently extracted, the content of effective ingredients is increased, and the healthcare effect is better.

The present invention relates to low and medium molecular weight isoflavone-β-D-glucan produced by submerged liquid culture of Agaricus blazei, a method of producing the isoflavone-β-D-glucan using autolysis enzyme of Agaricus blazei mycelia, and use of the isoflavone-β-D-glucan for anti-cancer and immunoenhancing effect.

The invention provides a nutrient composition and a preparation method thereof. The nutrient composition is prepared from, by weight, 100 parts of concentrated whey protein powder, 10-50 parts of fructo-oligosaccharide, 10-50 parts of galacto-oligosaccharide, 10-50 parts of polydextrose, 50-100 parts of whole milk powder, 0-15 parts of bovine colostrum powder, 0-15 parts of N-acetylneuraminic acid, 0-15 parts of yeast beta-glucan and 0-15 parts of oat beta-glucan. The nutrient composition contains various nutrients which can be easily digested and absorbed by human bodies, can comprehensivelysupplement various nutrients and can enhance human body resistance and increase immunity.

The invention relates to a chitosan containing antibacterial foaming agent for gynecology and a preparation method thereof, and belongs to the medicine field. The foaming agent is prepared from following raw materials in parts by weight: 0.5 to 2 parts of chitosan quaternary ammonium salt, 1 to 5 parts of chitosan, 0.5 to 2 parts of sodium hyaluronate, 0.5 to 1 part of 1,3-butylene glycol, 0.01 to1 part of oat beta-glucan, 0.2 to 2 parts of natural lactic acid, 2 to 5 parts of glycerin, 0.04 to 1 part of foaming agent, and 75 to 105 parts of purified water. The raw materials can generate a prominent synergistic effect on preventing pathogenic bacteria of colpitis. The foaming agent has a good antibacterial effect. Moreover, the irritation on vaginas is little, and the foaming agent is suitable for the treatments on gynecologic inflammation such as colpitis, cervicitis, and the like, and can be used to repair wounds of vagina mucosa.

The invention relates to an improved body fluid color-developing fungi 1,3-beta-D-glucan detection kit, which comprises a reaction main-agent, a main agent combination solution, a sample treatment fluid, sterile water, a standard substance and a quality control material. With Tachypleus tridentatus Leach or Limulus polyphemus Linnaeus hemocyte lysate as a main raw material, the reaction main-agent contains G factor, B factor, C factor, clottable protein and a polypeptide chromogenic substrate. By a new preparation process, the reaction main-agent has characteristics of high stability and small intra-assay and inter-assay difference. The main agent combination solution adopts a new formula. After the main agent combination solution is mixed with the reaction main-agent, Tachypleus Amebocyte Lysate endotoxin reaction branch can be effectively shielded. According to the kit, rate-method enzyme kinetics detection is carried out by ELIASA. The detection speed is fast, and anti-interference performance is strong. In addition, the preparation technology is simple, and the product has stronger stability and higher specificity and sensitivity.

The invention discloses a yeast beta-D-glucan derivative and a preparation method and an application thereof. The yeast beta-D-glucan derivative is obtained by substituting the hydroxyl of a yeast beta-D-glucan molecule with a sulfate group, wherein the degree of substitution of the sulfate group is 0.28-0.32 or 0.40-0.51. The invention further provides a method for preparing the yeast beta-D-dextran derivative, which comprises the following steps: dissolving yeast beta-D-glucan into an organic solvent and adding an esterifying agent for esterification reaction to obtain the yeast beta-D-dextran derivative. The contrastive analysis of a scanning electron microscopy discovers that the sulfated and modified yeast beta-D-glucan derivative is in a grid shape and has obvious grooves, the specific surface area is obviously increased, and the adsorption capacity is remarkably enhanced. An adsorption effect test shows that the adsorption quantity of zearalenone by the yeast beta-D-glucan derivative reaches 18.6528 mug / mg and is remarkably higher than that of the zearalenone by a yeast cell wall and the yeast beta-D-glucan.

The invention relates to a composition for balancing blood glucose. The composition comprises 15-20 parts of chia seeds, 45-55 parts of inulin, 15-20 parts of oat beta-glucosan, 5-10 parts of white kidney bean extract and 5-10 parts of sophora flower bud extract. The composition adopts multiple components for compatibility of medicines; the chia seeds, the inulin, the oat beta-glucosan, the white kidney bean extract and the sophora flower bud extract are compatible with one another, so that intake of most part of heat of starch can be blocked, an absorption process of saccharides can be retarded, intestinal tract movement is prompted, and thus the effect of balancing the blood glucose is achieved. The composition for balancing the blood glucose provided by the invention is simple in preparation method, effective components of various materials can be maximally reserved, and the effective components have a synergistic effect, gain mutually, and jointly achieve the effect of balancing the blood glucose.

The invention discloses a method for improving yeast beta-D-glucan water-solubility. The method comprises the steps that: (1) yeast beta-glucan is dissolved by using an ionic liquid, such that a glucan-ionic liquid mixed solution is obtained; (2) the glucan-ionic liquid mixed solution is subjected to a micro-jet homogenization process; (3) an organic solvent is added into the homogenized glucan-ionic liquid mixed solution, such that precipitation is carried out; (4) the precipitate is dissolved by using water, and centrifugation is carried out, such that a supernatant is obtained, and the improvement upon the yeast beta-glucan water-solubility is realized. With the method provided by the invention, modified and solubilized yeast beta-D-glucan is white fluffy cottony solid. With the method provided by the invention, yeast beta-glucan application range can be widened.

The invention relates to a sulphating method for improving water solubility of yeast beta-D-glucan. The method comprises steps as follows: 1) taking the yeast beta-D-glucan as a raw material, dissolving the yeast beta-D-glucan in a dimethyl sulfoxide solution containing urea, and obtaining a solution a after stirring and dissolution; 2) placing the solution a in an ice-bath, still placing the solution a in the ice-bath after ultrasonic treatment, and slowly dropwise adding a DMSO (dimethylsulfoxide) solution containing H2SO4 while stirring to obtain a solution b; 3) placing the solution b in an oil-bath constant-temperature vibrator for an esterification reaction, quickly cooling an esterified solution to the room temperature, adding deionized water, performing microfiltration by the aid of a polyester film after dilution, and then sequentially performing ultrafiltration treatment, concentration and drying to obtain the sulphated yeast beta-D-glucan. The sulphating method is combined with ultrasound, and the prepared yeast beta-D-glucan has the advantages of good solubility, high production safety, wide application range, capability of effectively enhancing body immune functions and the like.

The invention provides a preparation method of an oat fermentation extract, which comprises the following steps of: step 1, taking oat as a fermentation substrate, inoculating bifidobacteria, and carrying out anaerobic fermentation to obtain fermentation liquid; and step 2, inoculating saccharomycetes into the fermentation liquid, and carrying out aerobic fermentation to obtain the oat fermentation extract. According to the preparation method provided by the invention, the oat raw material is sequentially subjected to anaerobic and aerobic fermentation by utilizing bifidobacteria and saccharomycetes, and a fermentation control process is optimized, so that the oat extract rich in various active ingredients such as oat beta-glucan, glutathione, amino acid, lactic acid, total phenols, saponin and flavone is prepared, especially the content of beta-glucan and glutathione is obviously increased, and the content of amyloid sugar in the oat is reduced, so that the oat fermentation extract is particularly suitable for cosmetic raw materials, and an effective oat fermentation extract production process which is simple to operate is formed.

The invention relates to the technical field of detection of 1-3-beta-D glucan, and particularly discloses a simplified fungus 1-3-beta-D glucan detection kit. The kit comprises a kit body, an integrated reagent filling and reaction device, a reaction main agent, a main agent reconstitution fluid, a sample treating fluid and a quality control product; the integrated reagent filling and reaction device is composed of a reaction cup, a rear bin, a front bin and a push rod; and an integrated detection device can be formed by adding the reaction main agent, the main agent reconstitution solution and the sample treating fluid into the reaction cup, the rear bin and the front bin separately. The experiment operation is convenient, experiment tools are reduced, professionals are not needed, experiments are simple and rapid, and the detection accuracy is higher.