The invention relates to beta-1, 4-
glucanase and application thereof, and belongs to the technical field of
biology. A novel beta-1, 4-
glucanase is screened and identified in
Pleurotus tuber-regium, and the beta-1, 4-
glucanase has wide
substrate specificity, can specifically hydrolyze beta-
glucan (such as
sodium carboxymethyl cellulose) containing beta-1, 4 glucosidic bonds, and can hydrolyze beta-
glucan (such as
sodium carboxymethyl cellulose) containing beta-1, 3-1, 4-glucanase) containing beta-1, 3-1, 4-glucanase. The beta-1, 4-glucanase disclosed by the invention can be used for preparing beta-
glucan (such as barley beta-glucan and oat beta-glucan) containing beta-1, 3-1, 4 glucosidic bonds, so that the beta-1, 4-glucanase disclosed by the invention has good
enzyme activity on the beta-glucan containing the beta-1, 4 glucosidic bonds and the beta-glucan containing the beta-1, 3-1, 4 glucosidic bonds. Besides, the
enzyme activity of the beta-1, 4-glucanase disclosed by the invention can be kept at 70% or above under the condition that the pH value is 4.0-7.0, the
enzyme activity of the beta-1, 4-glucanase can be kept at 88% or above under the temperature of 40 DEG C, and the beta-1, 4-glucanase has good stability.