A composition for targeted removal of senescent cells and uses thereof

Abstract

The application discloses a composition for targeted removal of senescent cells and application thereof, and relates to the cosmetic technical field.The application provides a composition, which comprises component A and component B;the component A comprises camellia chrysantha extract, camellia pitardii flower extract and catechu extract; and the component B is oat beta-glucan.The camellia chrysantha extract, the camellia pitardii flower extract and the catechu extract are synergized with the oat beta-glucan, small molecules such as terpenes in the extract can be used as a natural penetration enhancer, and form a molecular channel with the oat beta-glucan; meanwhile, the above components can synergize to strengthen the removal of senescent cells, further strengthen cell regeneration, effectively remove cutin, brighten the skin, and play a role of mild anti-aging and brightening on sensitive skin.

Description

TECHNICAL FIELD

[0001] The present application relates to the cosmetic technology field, and in particular to a composition for targeted removal of senescent cells and application thereof. BACKGROUND

[0002] In recent years, skin anti-aging technology research mainly revolves around the following three technical paths: (1) antioxidant protection technology, based on the free radical oxidative damage theory, by exogenous supplementation of antioxidants such as vitamin C, vitamin E derivatives, tea polyphenols, etc. to neutralize active oxygen free radicals and reduce oxidative stress levels; (2) skin barrier repair technology, by supplementing ceramide, hyaluronic acid and essential fatty acids, etc. to reconstruct the lipid bilayer of the stratum corneum, maintain the water content and mechanical barrier function of the epidermis; (3) collagen regeneration promotion technology, using active ingredients such as retinoids, signal peptides and epidermal growth factor to stimulate dermal fibroblast proliferation, and improve collagen synthesis efficiency.

[0003] However, the existing technical system still has the following technical bottlenecks: first, the antioxidant ingredients have the technical defects of short half-life and low transdermal efficiency, which cannot effectively block the continuous generation of endogenous free radicals by the mitochondrial electron transport chain; second, the current scheme is mostly limited to epidermal structure repair, lacking systematic intervention in dermal extracellular matrix reconstruction, especially not involving targeted removal of senescent cells; third, a single mechanism is difficult to achieve multidimensional regulation of the aging process, such as not synchronously solving the intelligent construction of the transdermal channel, achieving dynamic balance of keratin metabolism and cell renewal, etc. Deep aging causes, resulting in short-term and superficial characteristics of anti-aging effect. The existing technical defects are mainly: 1) lack of recognition and removal strategy for deep senescent cells; 2) not synchronously solving the intelligent construction of the transdermal channel, not realizing intelligent exfoliation, etc.

[0004] Therefore, developing a new anti-aging system that can penetrate the epidermal barrier, target removal of senescent cells, and synchronously intelligent exfoliation has become a technical problem to be solved in the field. SUMMARY

[0005] Based on this, the purpose of the present application is to overcome the deficiencies of the prior art and provide a composition for targeted removal of senescent cells and application thereof.

[0006] To achieve the above purpose, the technical solution adopted by the present application is: a composition comprising component A and component B.

[0007] The component A comprises Camellia nitidissima extract, Camellia reticulata flower extract and catechin extract.

[0008] The component B is oat beta-glucan.

[0009] The present application is synergistic through Camellia sinensis var. assamica extract, Camellia pitardii flower extract and catechu extract. The polyphenols in Camellia sinensis var. assamica extract have the effect of scavenging free radicals, which can effectively slow down the cell aging process caused by oxidative stress. At the same time, flavonoids can inhibit the expression of aging-related genes. Tea polyphenols in Camellia pitardii flower extract have strong antioxidant effect, which can scavenge excess free radicals and reduce the damage to the skin caused by oxidative stress. The structure of tea polyphenols contains phenolic hydroxyl groups, which can effectively react with free radicals to form stable intermediates, thereby neutralizing the damage of free radicals to cells. In addition, flavonoids in Camellia pitardii flower have anti-inflammatory effect, which can reduce the inflammatory response of aging cells. Catechu extract is rich in EGCG (epigallocatechin gallate), ECG (epicatechin gallate) and EGA, which can improve the appearance of the skin, increase the elasticity of the skin, slow down the formation of wrinkles and reduce aging caused by free radicals through antioxidant, anti-inflammatory and skin cell regeneration promotion effects. Oat beta-glucan is a polysaccharide compound composed of multiple glucose units connected by beta-1,3 and beta-1,4 glycosidic bonds, which can form a protective film on the surface of the skin to lock in moisture.

[0010] The above three extracts are synergistic with oat beta-glucan. Small molecule terpenes in the extracts can act as natural penetration enhancers and form molecular channels with oat beta-glucan. At the same time, the above components can synergistically enhance the removal of aging cells and further enhance cell regeneration, intelligent exfoliation, skin lightening, and mild anti-aging and lightening effects on sensitive skin.

[0011] Preferably, the weight ratio of component A and component B is (2-15):1.

[0012] Further preferably, the weight ratio of component A and component B is (5-8):1.

[0013] The inventors found in actual experiments that the weight ratio of component A and component B further affects the effect of the composition in targeted removal of aging cells and intelligent exfoliation. When the weight ratio of component A and component B is within the above range, the composition has better effects in targeted removal of aging cells and intelligent exfoliation.

[0014] Preferably, the weight ratio of Camellia sinensis var. assamica extract, Camellia pitardii flower extract and catechu extract in component A is Camellia sinensis var. assamica extract: Camellia pitardii flower extract: catechu extract = (1-5): (1-5): (0.1-0.5).

[0015] Further preferably, the weight ratio of Camellia sinensis var. assamica extract, Camellia pitardii flower extract and catechu extract in component A is Camellia sinensis var. assamica extract: Camellia pitardii flower extract: catechu extract = (3-4): (2-3): (0.3-0.4).

[0016] The inventor found in the actual experiment that the weight ratio of the extract of Camellia chrysantha, the extract of Camellia pitardii and the extract of catechu in the component A would further affect the effect of the composition on targeted removal of senescent cells and intelligent exfoliation, and the effect of the composition on targeted removal of senescent cells and intelligent exfoliation was better when the weight ratio of the extract of Camellia chrysantha, the extract of Camellia pitardii and the extract of catechu in the component A was within the above range.

[0017] In the present application, the extract of Camellia chrysantha can be a water extract and / or an alcohol extract, which can be prepared according to the methods known in the art or obtained through commercial channels.

[0018] Preferably, the preparation method of the extract of Camellia chrysantha comprises the following steps: refluxing extraction of the whole plant of Camellia chrysantha with ethanol and / or water to obtain the extract of Camellia chrysantha.

[0019] In an embodiment, when the whole plant of Camellia chrysantha is extracted by refluxing with ethanol, the volume fraction of the ethanol is 50%-80%; and / or, the temperature of the refluxing is 60-80℃, and the time is 1-2h; and / or, the weight ratio of the whole plant of Camellia chrysantha to the ethanol is 1: (10-30).

[0020] In an embodiment, when the whole plant of Camellia chrysantha is extracted by refluxing with deionized water, the temperature of the refluxing is 60-80℃, and the time is 1-2h; and / or, the weight ratio of the whole plant of Camellia chrysantha to the deionized water is 1: (10-30).

[0021] The extract of Camellia chrysantha is mainly derived from the flowers, leaves, stems and roots of Camellia chrysantha, and the inventor found in the actual experiment that when the whole plant of Camellia chrysantha is extracted as raw material, flavonoids and polyphenols can be integrated, and further potential active ingredients such as amino acids, trace elements, theanine and polysaccharides in stems and roots can be integrated, thereby further enhancing the effect of intelligent cell renewal. When the whole plant of Camellia chrysantha is extracted as raw material, the effect of intelligent exfoliation is better when it is coordinated with the extract of Camellia pitardii, the extract of catechu and oat β-glucan.

[0022] Preferably, the number average molecular weight of the oat β-glucan is 40-100kDa.

[0023] The inventors found in practical experiments that the oat beta-glucan has a better synergistic effect with the Camellia nitidissima Chang extract, the Camellia reticulata Lindl extract and the catechu extract when the number average molecular weight of the oat beta-glucan is 40,000-100,000 Da, and the oat beta-glucan can better play the effect of intelligent exfoliation. When the number average molecular weight of the oat beta-glucan is too large, the molecular structure of the oat beta-glucan is complex, which leads to a decrease in the permeability of the oat beta-glucan on the skin surface or the cell membrane, and the oat beta-glucan is difficult to effectively penetrate the skin or combine with the cell receptors, thereby limiting the exertion of the biological activity of the oat beta-glucan. When the number average molecular weight of the oat beta-glucan is too small, the oat beta-glucan can more easily penetrate the skin, but the activity of the oat beta-glucan is also weakened. The small molecules lack sufficient three-dimensional structural stability and cannot form the required network structure to provide sustained protection, resulting in a decrease in the efficiency of the oat beta-glucan. When the number average molecular weight of the oat beta-glucan is 40,000-100,000 Da, the oat beta-glucan has a better effect of intelligent exfoliation.

[0024] In addition, the application provides application of the composition in preparation of cosmetics.

[0025] Further, the application provides a cosmetic comprising the composition; the cosmetic is one of the following: cosmetic water, emulsion, eye cream, face cream, face mask, essence, sunscreen, neck cream; and the dosage form of the cosmetic is one of the following: water agent, emulsion, jelly, spray, cream, essence.

[0026] The application provides an emulsion comprising the composition; specifically, the application provides an emulsion comprising the following components by weight percentage: thickening agent 0.05-0.5 parts, humectant 3-20 parts, emulsifier 0.5-10 parts, preservative 0.2-3 parts, pH regulator 0.05-0.1 parts, the composition 1-3 parts, and deionized water in excess.

[0027] Exemplarily, the emulsion comprises at least one of the following (a)-(e):

[0028] (a) the thickening agent comprises at least one of the following: xanthan gum, Carbopol 940, AVC, and high molecular cellulose;

[0029] (b) the humectant comprises at least one of the following: glycerol, butylene glycol, and low molecular sodium hyaluronate;

[0030] (c) the pH regulator comprises at least one of the following: arginine and EDTA-disodium;

[0031] (d) the emulsifier comprises at least one of the following: coco-caprylate / caprate, dimethicone, hydrogenated palm kernel oil, cetylstearyl alcohol, polyglyceryl-6 diisostearate, and methicone;

[0032] (e) the preservative comprises at least one of the following: 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0033] Compared with the prior art, the beneficial effects of the present application are: the present application synergizes the Camellia chrysantha Chang extract, the Camellia chrysantha Chang flower extract and the catechu extract, the polyphenols in the Camellia chrysantha Chang extract have the effect of scavenging free radicals, which can effectively slow down the cell aging process caused by oxidative stress, at the same time, the flavonoids can inhibit the expression of aging-related genes. The tea polyphenols in the Camellia chrysantha Chang flower extract have strong antioxidant effect, which can scavenge excess free radicals and reduce the damage to the skin caused by oxidative stress. The structure of tea polyphenols contains phenolic hydroxyl groups, which can effectively react with free radicals to form stable intermediates, thereby neutralizing the damage of free radicals to cells. In addition, the flavonoids in the Camellia chrysantha Chang flower have anti-inflammatory effect, which can reduce the inflammatory response of aging cells. The catechu extract is rich in EGCG (epigallocatechin gallate), ECG (epicatechin gallate), EGA, etc., which can improve the appearance of the skin, increase the elasticity of the skin, slow down the formation of wrinkles and reduce aging caused by free radicals through antioxidant, anti-inflammatory and promotion of skin cell regeneration. Oat beta-glucan is a polysaccharide compound composed of multiple glucose units connected by β-1, 3 and β-1, 4 glycosidic bonds, which can form a protective film on the surface of the skin to lock in moisture.

[0034] The above three extracts synergize with oat beta-glucan, the small molecule terpenes in the extracts can act as natural penetration enhancers and form molecular channels with oat beta-glucan; at the same time, the above components can synergize to enhance the removal of aging cells and further enhance cell regeneration, intelligent exfoliation, skin lightening, and have a mild anti-aging and lightening effect on sensitive skin. BRIEF DESCRIPTION OF DRAWINGS

[0035] Figure 1 Picture of β-galactosidase content in zebrafish in the model group, Example 1 and Comparative Example 1 in Performance Test-1;

[0036] Figure 2 Picture of wrinkle improvement before and after use of the application example 1, the comparative application example 10 and the blank application example in Performance Test-3. DETAILED DESCRIPTION

[0037] For better illustrating the purpose, technical scheme and advantages of the present application, the present application will be further explained in combination with the drawings and specific examples. The purpose is to understand the content of the present application in detail, instead of limiting the present application. All other examples obtained by the ordinary skilled in the art without making creative efforts are within the protection scope of the present application. The experimental reagents and instruments designed in the implementation and comparative examples of the present application are common reagents and instruments unless specially stated, and can be obtained from commercial channels. The experimental methods used in the implementation and comparative examples of the present application are conventional methods unless specially stated, and the raw materials used in parallel experiments are the same batch of raw materials unless specially stated.

[0038] The raw materials of the present application will be further explained as follows, but are not limited to the following raw materials:

[0039] Yunnan camellia flower extract: purchased from Hanshengyuan (Shaanxi) Biotechnology Co., Ltd.;

[0040] Catechu extract: purchased from Ningxia Xiangcao Biotechnology Co., Ltd.;

[0041] Camellia sinensis var. assamica extract-1: self-made, and the preparation method is as follows: collecting the whole plant of Camellia sinensis var. assamica, cleaning and drying in a 50℃ oven until constant weight, grinding and crushing through a No. 5 sieve, taking 1g, adding 20mL of 70% ethanol by volume, and refluxing at 70℃ for 1h to obtain the Camellia sinensis var. assamica extract;

[0042] Camellia sinensis var. assamica extract-2: self-made, and the preparation method is as follows: only the raw material is different, and the rest of the preparation method is completely the same; specifically, collecting the flowers of Camellia sinensis var. assamica.

[0043] Oat β-glucan-1: with a number average molecular weight of 60,000 Da, purchased from Mingsen Co., Ltd.;

[0044] Oat β-glucan-2: with a number average molecular weight of 10,000 Da, purchased from Huarui Biotechnology (Shanxi) Co., Ltd.;

[0045] Oat β-glucan-3: with a number average molecular weight of 200,000 Da, purchased from Shaanxi Zelang Biotechnology Co., Ltd.;

[0046] Honeysuckle extract: purchased from Xi'an Bingguo Biological Chemical Technology Co., Ltd.;

[0047] Black tea extract: purchased from Shaanxi Yuanbeibe Biological Technology Co., Ltd.;

[0048] Yeast β-glucan: purchased from Shaanxi Zelang Biotechnology Co., Ltd.

[0049] Examples and comparative examples

[0050] The present application provides a composition, the components and weight parts of the composition are selected as shown in Table 1 and Table 2, wherein the ratio M is the weight ratio of component A and component B, i.e. the total weight of Camellia nitidissima Chang extract, Camellia reticulata Lindl. flower extract and catechin extract to oat beta-glucan, i.e. (Camellia nitidissima Chang extract, Camellia reticulata Lindl. flower extract and catechin extract): oat beta-glucan; a preparation method of the composition, comprising the following steps: uniformly mixing the components to obtain the composition.

[0051] Table 1

[0052]

[0053] Table 2

[0054]

[0055] Application example

[0056] The present application provides an emulsion, the components (mass percentage) of the emulsion are shown in Table 3 wherein the compositions used in application examples 1-9 are respectively prepared in examples 1-9, and the compositions used in comparative application examples 1-9 are respectively prepared in comparative examples 1-9; for example, the composition used in application example 1 is prepared in example 1, the composition used in application example 2 is prepared in example 2, the composition used in application example 10 is prepared in example 1, and the composition used in comparative application example 1 is prepared in comparative example 1, and other analogies can be applied.

[0057] Table 3

[0058]

[0059] Performance test-1 removing senescent cells

[0060] Experimental materials: 15 mg of phenylthiocarbamide was dissolved in 10 mL of water to prepare a 10 mM stock solution, which was stored at 2-8°C in the dark. The working solution was prepared by diluting the phenylthiocarbamide stock solution 50 times with fish embryo culture solution to 0.2 mM, and freshly prepared before use to inhibit melanin production. Senescence-associated β-galactosidase kit (Cat No. KAA002RF, EMD Millipore Corp). Water for breeding parent fish: 4 g of sea salt was weighed and dissolved in 10 L of water to prepare, the salinity was 0.25%, the conductivity was 500 µS / cm, the dissolved oxygen was ≥80% saturation, the pH value was 7.0, and the hardness was 300 mg / L (calculated as calcium carbonate). Fish embryo culture solution: 2940 mg of anhydrous calcium chloride, 1233 mg of magnesium sulfate heptahydrate, 630 mg of sodium bicarbonate, and 55 mg of potassium chloride were dissolved in 10 L of water to prepare, the pH value was 6.5-8.5, and all chemicals were of analytical purity.

[0061] Experimental content: Wild-type AB strain zebrafish (Danio rerio) from a reliable source (such as the China National Zebrafish Resource Center) were used for oviposition testing. Healthy zebrafish embryos were selected and cultured at a density of no more than 1 embryo per 200 μL of fish embryo culture solution at 28°C until 2 days after fertilization.

[0062] Test blank control group: 24 fish embryos were randomly selected and placed in a 24-well plate, and 5 mL of fish embryo culture solution containing 0.2 mM phenylthiocarbamide was added. Model control group: 24 fish embryos were randomly selected and placed in a 24-well plate, and 5 mL of fish embryo culture solution containing 0.2 mM phenylthiocarbamide was added. Test group: 24 fish embryos were randomly selected and placed in a 24-well plate, and 5 mL of fish embryo culture solution containing 0.2 mM phenylthiocarbamide and the composition was added. Each test group of fish embryos was fixed in paraformaldehyde for 1 h, then treated with PBS for 5 min, and then washed with PBS for 1 h. The blank control group was not stained, and the rest of the test groups were stained with beta-gal for 4 h. Wash with PBS for 3 times, 5 min each time. Place the fish embryos on their sides. Then take photos under the same settings using a body microscope. The lighter the color, the less β-galactosidase content.

[0063] Data analysis: Open the photos with analysis software such as Image J, mark the yolk sac and intestinal region of each zebrafish embryo, then select "Measure Average Intensity" in the "Measure" column, and select the "average signal intensity" in the test results as the senescence-associated β-galactosidase level. Calculate the inhibition rate of senescence-associated β-galactosidase:

[0064] Inhibition rate = (S - M) / (B - M) x 100%

[0065] In the formula: S is the average value of the average signal intensity of the zebra fish embryos in the test group; M is the average value of the average signal intensity of the zebra fish embryos in the model control group; and B is the average value of the average signal intensity of the zebra fish embryos in the blank control group.

[0066] Test results: The compositions prepared in Examples 1-9 and Comparative Examples 1-9 were subjected to the removal of senescent cell test, and the test results are shown in Table 4 and Table 5. Figure 1 and Table 4.

[0067] Table 4

[0068]

[0069] As can be seen from the above table, the composition prepared in the embodiment of the present application has a good effect of targeted removal of senescent cells, and the inhibition rate of senescence-related β-galactosidase is more than 40%. Figure 1 As can be seen from the above table, the composition prepared in the embodiment of the present application has a good effect of targeted removal of senescent cells, and the inhibition rate of senescence-related β-galactosidase is more than 40%. Figure 1 As can be seen from the above table, the composition prepared in the embodiment of the present application has a good effect of targeted removal of senescent cells, and the inhibition rate of senescence-related β-galactosidase is more than 40%.

[0070] As can be seen from the comparison of Examples 1-6, the weight ratio of the component A and the component B, and the weight ratio of the Laurus nobilis extract, the Camellia reticulata extract and the catechin extract in the component A will further affect the effect of targeted removal of senescent cells of the composition, and the effect of targeted removal of senescent cells is better when the weight ratio is in the preferred range. As can be seen from the comparison of Examples 1, 7-9, the extraction raw material of the Laurus nobilis extract and the number average molecular weight of the oat β-glucan in the composition have little effect on the effect of targeted removal of senescent cells, and all have a good effect of targeted removal of senescent cells, and the inhibition rate of senescence-related β-galactosidase is more than 50%.

[0071] As can be seen from the comparison of Examples 1 and Comparative Examples 1-6, when one of them is not added, or when the other components are added to make up for the missing part, the effect of the present application cannot be achieved; as can be seen from Examples 1 and Comparative Examples 7-9, when other similar components are used to replace, the corresponding effect of the present application cannot be achieved.

[0072] Performance test-2 intelligent exfoliation

[0073] Test process: cells: human epidermal keratinocytes (KC), purchased from Guangdong Boxi Biological Technology Co., Ltd.; main reagent: KC Growth culture medium; main instrument: CO2 incubator, clean bench, inverted microscope; cell inoculation: 4×10 5Inoculation density: Cells were inoculated to 6-well plates at a density of 1x10~4 cells / well and incubated in an incubator (37℃, 5% CO2) for 24h. The sample was diluted to 0.5% (v / v) with the culture medium. After the sample was added, the cells were incubated for 1min, 5min, 10min, 15min and 30min, respectively. After the incubation, the cell culture solution was collected and centrifuged to calculate the cell number. The specific operation was based on the laboratory method SOP-FUN-058 in vitro keratolytic efficacy standard operating procedure.

[0074] Test results: The compositions prepared in Examples 1-9 and Comparative Examples 1-9 were subjected to keratolytic tests, and the test results are shown in Table 5.

[0075] Table 5

[0076]

[0077] As can be seen from the above table, the compositions prepared in the examples have good intelligent keratolytic effects. In the keratolytic test process, the cell number was 40x10~4 after the sample was added and incubated for 15min, which was 10x10~4 higher than that after the sample was added and incubated for 30min. 4 The difference between the cell number after the sample was added and incubated for 30min and the cell number after the sample was added and incubated for 15min was less than 10x10 4 The difference was very small, which could achieve the intelligent effect of effectively cleaning keratin without over-cleaning keratin.

[0078] As can be seen from the comparison of Examples 1-6, the weight ratio of the component A and the component B, and the weight ratio of the Laurus nobilis extract, the Camellia chingii extract and the catechin extract in the component A further affect the intelligent keratolytic effect of the composition. When the weight ratio is in the preferred range, the intelligent keratolytic effect is better. Specifically, when the weight ratio is in the preferred range, the cell number increases more obviously after the sample is added and incubated for 15min, and the difference between the cell number after the sample is added and incubated for 30min and the cell number after the sample is added and incubated for 15min is less obvious, which basically remains unchanged, and the intelligent effect of effectively cleaning keratin without over-cleaning keratin can be better achieved. As can be seen from the comparison of Examples 1, 7-9, the extraction raw material of the Laurus nobilis extract and the number average molecular weight of the oat beta-glucan in the composition have certain influence on the intelligent keratolytic effect. When the whole plant of Laurus nobilis is used as the raw material for extraction and / or the number average molecular weight of the oat beta-glucan is 40-100kDa, the cell number increases more obviously after the sample is added and incubated for 15min, and the cell number after the sample is added and incubated for 30min and the cell number after the sample is added and incubated for 15min basically remain unchanged, and the intelligent effect of effectively cleaning keratin without over-cleaning keratin can be better achieved.

[0079] From the comparison of Example 1 and Comparative Examples 1-6, when one or two components are not added, or when other components are added to make up the parts, the effects of the present application cannot be achieved. From Example 1 and Comparative Examples 7-9, when other similar components are used to replace, the corresponding effects of the present application cannot be achieved.

[0080] Clinical test of Test Example 3

[0081] According to the test of the “Cosmetic Safety Technical Specification” (2015), 30-55 year-old sensitive skin volunteers (lactic acid stinging score > 3) were selected and randomly divided into 4 groups, 10 people in each group. The test area was washed with water without applying any product, and the volunteers sat in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes to test the skin condition before use. The volunteers used an appropriate amount of emulsion on their faces every morning and evening after cleansing and moisturizing, massaged and absorbed, once in the morning and once in the evening, and returned on the 7th, 14th and 28th days. The volunteers visited the test area and washed their faces with water without applying any product, and sat in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 20 minutes. The instrument was used to quantify and take pictures of the improvement of facial indicators. Including Cutometer 2mm MPA 580 test skin elasticity, Primos CR(5.8E) test skin wrinkles, VC 20 Plus test skin roughness.

[0082] Improvement rate before and after use = |X mean before use - X mean after use| / X mean before use x 100%.

[0083] Test results: The experimental results of Application Example 1, Application Example 10, blank application example and Comparative Application Example 1 are shown in Table 5 and Table 6. Figure 2

[0084] Table 6

[0085]

[0086] As can be seen from the above table, when the composition of the present application is applied to the emulsion, the mass percentage content of 1-3% can achieve good effects of improving skin elasticity, improving wrinkles and reducing skin roughness. From the comparison of Application Example 1 and Comparative Application Example 1, when one of them is not added, the effects of improving skin elasticity, improving wrinkles and reducing skin roughness cannot be achieved. Figure 2 For the wrinkle improvement chart of Application Example 1, Comparative Application Example 10 and blank application example before and after use, it can be seen that the emulsion prepared by the application example has the effects of improving skin elasticity, improving wrinkles and reducing skin roughness.

[0087] ​Finally, it should be noted that the above examples are merely intended to illustrate the technical solutions of the present application and not to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A composition, characterized in that, It is composed of component A and component B, wherein the weight ratio of component A to component B is (5-8):1; Component A consists of Camellia chrysantha extract, Camellia yunnanensis flower extract, and catechu extract, wherein the weight ratio of Camellia chrysantha extract, Camellia yunnanensis flower extract, and catechu extract in component A is Camellia chrysantha extract: Camellia yunnanensis flower extract: Catechu extract = (1-5): (1-5): (0.1-0.5). Component B is oat β-glucan; The preparation method of the Camellia chrysantha extract includes the following steps: extracting the whole Camellia chrysantha plant by reflux with ethanol and / or water to obtain the Camellia chrysantha extract.

2. The composition according to claim 1, characterized in that, The weight ratio of Camellia chrysantha extract, Camellia yunnanensis flower extract and catechu extract in component A is Camellia chrysantha extract: Camellia yunnanensis flower extract: catechu extract = (3-4): (2-3): (0.3-0.4).

3. The composition according to claim 1, characterized in that, The volume fraction of the ethanol is 50%-80%; And / or, the reflux temperature is 60-80℃ and the time is 1-2h.

4. The composition according to claim 1, characterized in that, The number-average molecular weight of the oat β-glucan is 40,000-100,000 Da.

5. The use of a composition according to any one of claims 1-4 in the preparation of cosmetics.

6. A cosmetic product, characterized in that, The cosmetic includes the composition according to any one of claims 1-4; the cosmetic is one of toner, lotion, eye cream, face cream, face mask, serum, sunscreen, and neck cream.

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