56 results about "Calcium supplement" patented technology

The invention discloses a peptide with a bone strengthening effect as well as a preparation method and application thereof, and relates to the field of biology. The casein is used as a raw material, the high-activity calcium ion chelating peptide and the directional preparation technology thereof are provided, the technology has the advantages of being high in efficiency, low in cost, easy to operate, good in stability and the like, the high-activity calcium ion chelating peptide can be prepared on a large scale, the utilization value of the casein is improved, and a new way is provided for development of functional natural calcium supplement products.

The present application relates to a kind of calcium peptide chelate based on defatted sheep bone powder homologous recombination and its preparation method and application, preparation method, including the following steps, defatted sheep bone powder is mixed with water, flavourzyme is added to carry out enzymolysis, enzyme is inactivated after enzymolysis, and the enzymatic hydrolysate containing bone-derived peptide is obtained;Lactic acid is added in enzymatic hydrolysate and is hydrolyzed, so that calcium ion is dissolved, and the mixed solution containing bone-derived peptide and calcium ion is obtained;Mixed solution is carried out chelation under neutral condition, after reaction, aftertreatment is carried out, and calcium peptide chelate is obtained;Calcium peptide chelate prepared according to the above preparation method is used as salty taste enhancer, salt-reducing auxiliary agent or flavor enhancer, and its application in food.The present application has the advantages of homologous resource recombination, improves chelation rate, raw material utilization rate is high, realizes sheep bone by-product high value, process safety, can add chelate to clear soup base material, so as to reduce sodium, the effect of calcium supplement, salty seasoning.

This invention discloses a method for in-situ preparation of calcium chelate peptides that promote intestinal calcium absorption and osteoblast development from fish scales using high-pressure homogenization-assisted enzymatic hydrolysis. The method uses fish scales as raw material, which are pulverized and then pretreated with high-pressure homogenization to break down the dense cross-linked structure of the scales. Pepsin is used as the hydrolytic enzyme to achieve efficient enzymatic hydrolysis of collagen and in-situ chelation with natural calcium, resulting in a one-step preparation of collagen calcium chelate peptides. The product has a calcium retention rate of ≥84.16%, is rich in affinity amino acids, and shows a ≥62.97% increase in calcium transport compared to calcium chloride in a small intestinal epithelial cell absorption and transport model, while being safe and non-cytotoxic. This invention is a green and efficient process that eliminates the need for decalcification steps, achieving a collagen calcium chelate peptide yield of ≥83.4%. It realizes the high-value utilization of aquatic by-products and can be directly applied to various calcium supplements such as food and health products that enhance intestinal calcium absorption and promote bone development, solving problems such as low bioavailability of traditional calcium preparations and severe calcium loss in existing fish scale utilization processes.

The invention discloses a method for preparing a phosphorylated salmon collagen peptide-calcium chelate by taking salmon collagen peptide as a raw material, preparing high-calcium binding active peptide (P-CP) through phosphorylation modification and chelating the high-calcium binding active peptide with calcium ions. The method comprises the following steps: (1) enzymolysis preparation of salmon bone collagen; (2) phosphorylation modification of a peptide fragment mediated by sodium tripolyphosphate; and (3) optimizing a calcium ion chelation process. The chelating rate of the obtained P-CP-Ca calcium reaches 91.21%, and structural characterization proves that a phosphate group (PO4 < 2->) is combined with calcium ions through ionic bonds. Animal experiments show that P-CP-Ca can significantly improve the serum calcium level of a calcium-deficiency mouse (the H group recovers to a normal range), reduce the activity of a bone resorption marker TRAP (Target Related Amplified Protein) (which is 25.4% lower than that of a model group), and improve the bone trabecula microstructure (the bone mineral density is improved by 32.7%). The invention provides a new technology for valuable utilization of fishery by-products and development of functional calcium supplements.

The invention belongs to the technical field of medicinal preparation processing, and particularly relates to oral liquid for supplementing calcium, vitamin D and vitamin K and a preparation method of the oral liquid. The fruit and vegetable powder is prepared from the following raw materials in parts by weight: 100 to 500 parts of calcium citrate, 0.2 to 2.5 parts of vitamin D3 emulsion, 0.4 to 5.5 parts of vitamin K2, 0 to 100 parts of casein phosphopeptides, 0.1 to 200 parts of fruit and vegetable powder, 0 to 5 parts of citric acid, 0 to 5 parts of malic acid, 0.1 to 400 parts of a sweetening agent, 0.01 to 10 parts of a thickening agent, 0.1 to 5 parts of an emulsifying agent, 0 to 2 parts of stevioside and 665 to 1395 parts of water. According to the invention, the vitamin D3 emulsion process is improved, the proper antioxidant is added, and the proper consistency is adjusted, so that the stability of the vitamin D3 in the oral liquid product is ensured, and the dosage form application range of the calcium supplement is enlarged.

The invention discloses a cultivation method for enhancing the total amount of bio-available calcium of edible parts of pakchoi, and relates to the technical field of Chinese cabbage cultivation, and the method comprises the following steps: variety screening, seedling raising, transplanting, foliar fertilizer spraying, cultivation and picking. Through the design of high-calcium variety screening, calcium nutrition pretreatment in the seedling raising period, foliar calcium supplement and potassium fertilizer synergistic application, the calcium content of the edible part of the pakchoi is increased, more importantly, the absorption, transport and accumulation process of calcium in the plant body is optimized, the total amount of bio-available calcium which can be actually absorbed and utilized by the human body in the edible part is increased, and the yield of the pakchoi is increased. The problem that an existing calcium fertilizer application and biological enhancement technology cannot guarantee effective calcium absorption of a human body is solved. Aiming at the problems of low absorption efficiency of traditional soil calcium fertilizer application and lack of systematicness of a foliar calcium fertilizer technology, through pretreatment of an EDTA calcium complex in a seedling raising period, precipitation which is difficult to absorb and formed by combination of calcium and other substances in soil or a matrix is avoided, and an efficient calcium absorption basis is constructed for seedlings.

The invention discloses a preparation method of a high-absorptivity calcium supplement of vitamin D3 (VD3) and casein phosphopeptide chelated calcium (CPP-Ca), which comprises the following steps: firstly, dissolving casein phosphopeptide (CPP) in ultrapure water, adding calcium chloride, adjusting the pH value, stirring and reacting at a certain temperature to generate CPP-Ca, then precipitating by using ethanol, washing for multiple times to remove free calcium ions, and drying to obtain the high-absorptivity calcium supplement of vitamin D3 (VD3) and casein phosphopeptide chelated calcium (CPP-Ca). Centrifuging, collecting the precipitate, and freeze-drying to obtain purified CPP-Ca; the preparation method comprises the following steps: preparing CPP-Ca into a solution, adjusting the pH value, dropwise adding VD3 dissolved in ethanol into the solution, stirring and mixing at room temperature under a certain concentration to finally form the VD3 and CPP-Ca compound, and the compound prepared by the method can significantly promote calcium absorption and effectively improve the stability of VD3 and CPP.

This invention belongs to the field of medical materials, specifically relating to a patterned bio-barrier membrane with self-adhesive function and its preparation method. The preparation method includes: dissolving the adhesive matrix in a buffer solution, then adding reactive amino acids and a stabilizer to obtain a first mixture; adding ethanol and a hydrophobic agent to the first mixture, and stirring under heating conditions to obtain a second mixture; adding a thickener, a calcium supplement, and a reinforcing agent to the second mixture, continuing stirring, and then adding a protein precipitation reagent to precipitate the mixture, obtaining a hydrogel; covering a collagen membrane with a patterned porous mold, and adding the hydrogel to allow it to flow into the pores and solidify. This invention achieves precise adhesive loading through patterned design, combining advantages such as simple operation, reliable fixation, good biocompatibility, and high osteogenic efficiency, effectively solving the barrier membrane fixation problem in GBR technology, and is suitable for bone defect treatment in dental implant prosthesis restoration.

The present application relates to the technical field of aquaculture, and particularly relates to a denitrification agent for aquaculture and a preparation method thereof, which comprises the following raw materials in 100% by weight: modified polymeric ferric sulfate 24-26%, iron hydroxide 36-38%, polymeric aluminum ferric silicate 1.5-2%, calcium supplement 3-3.5%, and the rest is maifanite powder. The denitrification agent has a good water improvement effect, and can effectively remove pathogenic bacteria in water for a long time.

The present application provides a kind of polycyclic carbamoyl pyridone derivative pharmaceutical preparation and preparation method thereof, the pharmaceutical preparation includes the compound shown in formula (I) or its pharmaceutically acceptable salt and diluent, wherein the particle size D90 of the compound shown in formula (I) or its pharmaceutically acceptable salt is 0.5-50 μm.The polycyclic carbamoyl pyridone derivative pharmaceutical preparation obtained by the present application has high dissolution rate, good stability, oral absorption is not affected by food, can be used with dairy products, calcium supplement food, good compliance, suitable for industrial production.

This invention discloses a lactobridged protein-derived calcium chelating peptide and its applications, belonging to the field of bioactive small molecule peptide technology. The calcium chelating peptide provided by this invention is safe and non-toxic, exhibiting better physicochemical activity compared to traditional calcium supplements. Its calcium chelating capacity reaches over 419.36 μg / mg, and it can promote calcium transport in the Caco-2 cell monolayer, thereby improving calcium absorption and bioavailability in the human body, making it suitable for use as a calcium supplement. This invention can develop a new protein source for calcium supplements and makes a significant contribution to the high-value utilization of dairy products.

The invention belongs to the field of calcium protein, and discloses sea cucumber source high-absorbability calcium protein and a preparation method thereof.The sea cucumber source high-absorbability calcium protein is prepared from sea cucumbers, and viscera of the sea cucumbers are not removed when the calcium protein is prepared; 1 part by weight of calcium protein is prepared from 50 parts by weight of sea cucumbers; the preparation method comprises the following steps: S1, raw material pretreatment; s2, primary processing; s3, performing low-temperature freeze-drying; s4, crushing and sterilizing; s5, removing fishy smell and granulating; s6, filling and packaging capsules; according to the technical scheme, 100% pure natural sea cucumbers are used as the unique raw material to prepare the calcium protein, no chemical additives, preservatives, sweeteners and the like are used, the method is green and healthy, and the practicability and safety are improved; meanwhile, the applicability of the calcium protein is greatly improved, the calcium supplement product is suitable for different people to use, the problem that a conventional calcium supplement product cannot be absorbed is solved, the price is reasonable, the preparation process is simple and convenient, and industrial production and popularization are easy.

The invention provides a micro-fat calcium supplement carrier, which is prepared from the following raw materials in parts by weight: 30 to 35 parts of standard index calcium, 1 to 5 parts of chondroitin, 1 to 5 parts of glucosamine, 8 to 12 parts of pure camel milk, 0.7 part of vitamin D, 2 to 70.3 parts of vitamin K, 1 to 3 parts of phospholipid complex and 8 to 10 parts of excipient. According to the invention, standard calcium, chondroitin, glucosamine, pure camel milk, vitamin D, vitamin K2-7, a phospholipid complex and an excipient are organically mixed by adopting a liposome carrier technology, so that the digestion and absorption of calcium in a body and the distribution of calcium in a specific region are effectively improved, and further the effects of rapid bone growth, increase of bone length (promotion of height increase) and increase of bone density are achieved; the health-care food has the health-care functions of reducing the fracture probability, preventing tooth decay, reducing the death rate of cardiovascular diseases and the like, so that the calcium absorption rate is remarkably improved and reaches up to 95%.

This invention discloses an ultrafine organic calcium supplement solid beverage and its preparation method. It comprises a carrier powder derived from natural plants, ultrafine organic calcium source powder, and ultrafine low glycemic index sweetener powder. The particle size distribution of the organic calcium source ultrafine powder is D90≤10μm. All three components form a uniform mixed powder system. This invention significantly increases the specific surface area of ​​the calcium powder through physical ultrafine pulverization technology. Combined with a specific dry mixing process, it solves the technical problems of existing calcium supplement beverages, such as complex formulations, high calcium source irritation, uneven raw material dispersion, and poor reconstitution performance. The product formula of this invention is extremely simple and pure. Using an organic calcium source, it can be efficiently absorbed without the participation of gastric acid, is gentle and non-irritating to the stomach and intestines, and has a smooth, clumpy taste after reconstitution. Its preparation method is simple, energy-efficient, and easily scaled up industrially. The resulting solid beverage is suitable for all age groups, especially children, people controlling their blood sugar, and middle-aged and elderly people with sensitive stomachs, as a daily dietary calcium supplement.

The invention provides a calcium supplement and a preparation method thereof, the calcium supplement comprises sugar alcohol, calcium salt, an aqueous solvent and an oily solvent, C sugar alcohol represents the concentration of the sugar alcohol in the calcium supplement, CH < + > represents the hydrogen ion concentration in the calcium supplement, Ksp represents the dissolution equilibrium constant of the calcium salt at 25 DEG C, the calcium supplement meets the condition that Ksp * CH + / C sugar alcohol is equal to 3.7 * 10 <-33 >-1.4 * 10 <-8 >. According to the calcium supplement, by controlling the Ksp value of calcium salt and comprehensively regulating and controlling the concentration of sugar alcohol and hydrogen ions, synergistic defoaming can be achieved, the quality stability is improved, and the shelf life is prolonged.

The invention provides a mushroom-sourced functional polypeptide capable of being combined with calcium ions and promoting calcium absorption, relates to the technical field of biology, and particularly provides a separated polypeptide which has an amino acid sequence as shown in SEQ ID NO: 2, 3 and / or 4. The polypeptide not only can be efficiently combined with calcium ions, but also has the activity of promoting calcium ion absorption. Therefore, the polypeptide can be further developed into functional foods such as polypeptide-calcium supplements and the like to meet the requirements of people on novel supplements.

The invention discloses a preparation method of glycosylated bamboo shoot peptide chelated calcium. Combining the bamboo shoot cleaning solution with the filtrate, carrying out alkali dissolution and acid precipitation, collecting precipitates, dispersing the precipitates in ultrapure water, and carrying out spray drying to obtain bamboo shoot protein powder; taking the bamboo shoot protein, adding alkaline protease, carrying out enzymolysis reaction, and carrying out ultrafiltration and freeze drying; the preparation method comprises the following steps: mixing BSH with six different saccharides, and dissolving in deionized water to prepare a solution; preparing BSH with similar grafting degrees and same time; cooling, filtering, and freeze-drying trapped fluid to obtain a BSH sample; mixing the glycosylated BSH solution with calcium chloride, and adding absolute ethyl alcohol to obtain glycosylated BSH-Capowder; the calcium binding capacity of the bamboo shoot peptide is effectively improved, an experimental basis is provided for development of BSH-Ca and XY-BSH-Ca as calcium supplements and functional peptide additives, the high-value utilization approach of bamboo shoot resources is expanded, and a new thought is provided for research and development of novel efficient calcium supplements.

This invention discloses a collagen calcium chelating peptide and its application in the preparation of calcium supplements and osteoporosis prevention products, belonging to the field of food biotechnology. Osteogenic activity evaluation confirmed that the collagen calcium chelating peptide significantly promoted MC3T3-E1 cell proliferation, increased alkaline phosphatase activity, and enhanced mineralized nodule formation within a concentration range of 1-100 μg / mL, exhibiting a clear concentration-dependent effect. Intestinal calcium absorption promotion experiments showed a significant increase in calcium transport compared to the calcium chloride control group, and fluorescence imaging confirmed its effective enhancement of calcium ion uptake in small intestinal epithelial cells. This invention clarifies for the first time the structural characteristics and dual biological activities of a fish scale-derived calcium chelating peptide, revealing its mechanism of action in regulating osteoblasts through the EGFR signaling pathway. This provides a new pathway for the high-value utilization of aquatic by-products and offers a scientific basis and high-quality candidate raw materials for the development of novel functional calcium supplements.

The invention relates to the technical field of aquaculture, in particular to a denitrification agent for aquaculture and a preparation method thereof, and the denitrification agent is prepared from the following raw materials in percentage by weight: 24-26% of modified polyferric sulfate, 36-38% of ferric hydroxide, 1.5-2% of polyaluminum ferric silicate, 3-3.5% of calcium supplement and the balance of medical stone powder. The nitrogen removal agent disclosed by the invention has a relatively good water body improvement effect and can effectively remove pathogenic bacteria in a water body for a long time.

The invention provides a biological nano calcium supplement capsule and a preparation method thereof. The biological nano calcium supplement capsule comprises egg shells and oatmeal. According to the biological nano calcium supplement capsule prepared by the method disclosed by the invention, the solubility of calcium is relatively high, and the calcium can be quickly absorbed by a human body.

The invention provides a method for preparing a calcium-rich product through fermentation of enterococcus faecium and application, calcium is enriched through fermentation of enterococcus faecium, ultrasonic treatment is carried out in the fermentation process, the calcium-rich capacity of enterococcus faecium is improved, the influence of calcium ions on the physiological activity of enterococcus faecium is researched, and the calcium-rich product is prepared by adjusting the variety and concentration of a carbon source and a nitrogen source. The formula of the fermentation culture medium is optimized, growth of the enterococcus faecium is promoted, the capability of converting inorganic calcium into organic calcium is enhanced, meanwhile, the antioxidant activity can be improved, and the enterococcus faecium fermentation culture medium has important significance on development of novel calcium supplements.

The application provides a calcium supplement and a product using the same, the calcium supplement comprising xanthan gum, a calcium salt, an aqueous solvent, and an oily solvent, the xanthan gum having an acetone group content of 1-10 wt%, an acetyl group content of 2-5.5 wt%, and the calcium supplement having a pH value of 3.0-5.0. The calcium supplement balances the processing fluidity and storage stability of the calcium supplement by regulating the acetone group content and the acetyl group content of the xanthan gum and the pH value of the calcium supplement, so that the calcium supplement exhibits a low viscosity characteristic of easy flow under the required shear force for filling, facilitating the filling operation, and in a static storage state, the system can quickly recover and maintain a high viscosity, thereby effectively preventing the calcium salt particles from settling and the oil phase from floating, and significantly improving the stability and uniformity of the product.

This invention discloses a method for preparing viscous bone cement, comprising the following steps: S1: mixing a silk fibroin solution with a natural polymer solution to obtain a mixed solution; S2: processing the mixed solution obtained in step S1 to obtain powder A; S3: selecting a calcium-containing inorganic compound as a basic calcium salt, combining it with calcium supplement raw materials to adjust the calcium-to-phosphorus ratio to 1.67, and mixing the basic calcium salt with the calcium supplement raw materials to obtain calcium salt powder B; S4: mixing powder A obtained in step S2 with calcium salt powder B obtained in step S3 according to the mixing ratio to obtain solid powder C; this invention uses tricalcium phosphate and calcium dihydrogen phosphate as the curing substrate, introducing biodegradable silk fibroin and gelatin into the curing system, and the gelatin and silk fibroin carrying positive and negative charges respectively, and their electrostatic interaction giving them viscosity, providing a viscous substrate.

Disclosed is the use of dithiocarbamate compounds in the preparation of a drug for preventing and / or treating osteoporosis. The dithiocarbamate compounds can effectively prevent and / or treat osteoporosis, improve bone mineral density in patients with osteoporosis, and reduce the risk of fractures. Moreover, the dithiocarbamate compounds, when used in combination with a calcium supplement, can achieve better preventive and / or therapeutic effects on osteoporosis.

The invention discloses a preparation method of viscous bone cement, which comprises the following steps: S1, mixing a silk fibroin solution with a natural polymer material solution to obtain a mixed solution; s2, treating the mixed solution prepared in the step S1 to obtain powder A; s3, selecting a calcium-containing inorganic compound as a basic calcium salt, matching the basic calcium salt with a calcium supplement raw material to regulate the ratio of calcium to phosphorus to 1.67, and mixing the basic calcium salt with the calcium supplement raw material to obtain calcium salt powder B; s4, the powder A prepared in the step S2 and the calcium salt powder B prepared in the step S3 are mixed according to the mixing proportion, and solid-phase powder C is obtained; tricalcium phosphate and monocalcium phosphate are selected as curing substrates, degradable silk fibroin and gelatin are introduced into a curing system, and the gelatin and the silk fibroin respectively have positive and negative charges, so that the adhesive has viscidity through electrostatic interaction, and a viscidity substrate is provided.

The invention discloses application of animal bifidobacterium in products for improving bone health and growth and development, and belongs to the technical field of microorganisms. The action mechanism of a traditional calcium supplement mainly depends on physical supplement of exogenous calcium, but the improvement of the absorption efficiency of intestinal tract calcium is limited, and a molecular regulation and control network of bone metabolism cannot be intervened, the invention provides a bifidobacterium animalis BB211 with the preservation number of GDMCC NO: 67124, and the bifidobacterium animalis BB211 can be used for preparing a bifidobacterium animalis strain BB211 with the preservation number of GDMCC NO: 67124. The strain can significantly improve the serum calcium level without depending on exogenous calcium supplement, synchronously improves the balance of bone formation and absorption, and has a unique metabolic regulation advantage.

The invention discloses a preparation method of a citric acid grafted casein phosphopeptide calcium compound. The preparation method comprises the following steps: 1, dissolving casein phosphopeptide in a 0.1 mol / L MES buffer solution with the pH value of 5.5; 2, dissolving citric acid according to a mass ratio of the citric acid to the casein phosphopeptides of 1: 1, adding a reagent according to a ratio of CA: EDC: NHS of 1: 1.5: 1.2, and activating in an ice bath at 4 DEG C for 30 minutes; 3, mixing the two solutions, reacting at room temperature for 24 hours, dialyzing, and freeze-drying to obtain CA-CPP; and 4, dissolving CA-CPP in water, adding calcium acetate monohydrate to adjust the pH value to 7.0, carrying out a reaction at 50 DEG C for 60 min, carrying out ethanol precipitation, and carrying out centrifugation and freeze-drying to obtain the product. The calcium chelating ability and stability are improved, the process is simple and safe, and the method is suitable for functional food and calcium supplements.

The invention provides a high-dissolution-rate and high-absorptivity liquid calcium preparation as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: carrying out superfine grinding on food-grade calcium citrate, carrying out ultrasonic treatment, loading in milk exosome, mixing with liposome loaded with vitamin K2 and vitamin D3, carrying out ultrasonic treatment, incubating to obtain a fusion body, mixing the fusion body with collagen peptide chelated with calcium, carrying out ultrahigh-pressure homogenization treatment, filtering, sterilizing and filling to obtain the collagen peptide containing vitamin K2 and vitamin D3. The liquid calcium preparation with the high dissolution rate and the high absorption rate is obtained. By improving the production process and the preparation, the liquid calcium preparation product which can stably exist for a long time and is high in dissolution rate and absorptivity is prepared, the absorptivity of calcium supplement function nourishment is improved, the calcium supplement effect of the product is further improved, and the preparation has a good application prospect.

The invention discloses an application of a calcium-reinforced layered network structure emulsion in preparation of a product for promoting calcium absorption and enhancing bone mineral density, and aims to solve the technical bottlenecks of low absorption rate and insufficient bone targeting of a traditional calcium supplement. According to the emulsion, the insoluble calcium salt is uniformly dispersed in an oil phase by utilizing the density difference between the insoluble calcium salt and the oil phase, and is embedded into a layered network structure formed by proteoglycan. Hydrophobic molecules or hydrophobic chain segments spontaneously gather to form a hydrophobic region, and free diffusion of water molecules and water-soluble ions is effectively prevented, so that solubilization and stable dispersion of insoluble calcium salt are realized. The obtained emulsion has good processing stability and tolerance, the absorption rate of calcium is remarkably improved, and the bone mineral density is favorably enhanced. The system can be used as a calcium fortifying preparation to realize on-line addition, the process is simple and convenient, the system is suitable for various foods such as dairy products, biscuits, ice cream and plant milk, and preparation and application of high-calcium nutritious foods are realized.

The invention belongs to the technical field of functional food and functional active substances, and particularly relates to a high-calcium binding capacity active peptide as well as a screening method and application thereof. The amino acid sequence of the active peptide with the high calcium binding capacity is as shown in SEQ ID NO. 1, or as shown in SEQ ID NO. 2, or as shown in SEQ ID NO. 3. The high-calcium binding capacity active peptide provided by the invention comprises a peptide YEGVIL containing one glutamic acid, a peptide YEVIL containing two glutamic acids, and a peptide YEVEIL containing two glutamic acids. Compared with peptides from other sources, the active peptide provided by the invention has more excellent calcium chelation capacity, the calcium binding capacity reaches 270 mu g / mg or above, and the peptide-calcium chelate prepared from the active peptide is easier to absorb, is suitable for preparation and use of the peptide-calcium chelate, and can provide better raw material selection for a novel calcium supplement.