Method for preparing low-molecular-weight sheep bone collagen polypeptide calcium chelate microcapsules

A collagen peptide and low molecular weight technology, applied in the biological field, can solve the problems of not taking into account the molecular weight and chelation rate of the peptide, and the inability to supplement collagen and calcium together, and achieve low cost, high solubility, and operability strong effect

Inactive Publication Date: 2012-05-02
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the shortcomings of the above-mentioned calcium supplement preparations have been solved, it cannot achieve the same supplementation of collagen and calcium.
Application No. 200810044574.3 for "Silver Bovine Bone Chelate and Its Preparation Method and Application" and Application No. 201110129187.1 for "Deer

Method used

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  • Method for preparing low-molecular-weight sheep bone collagen polypeptide calcium chelate microcapsules
  • Method for preparing low-molecular-weight sheep bone collagen polypeptide calcium chelate microcapsules

Examples

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Embodiment 1

[0020] A preparation method of low molecular weight sheep bone collagen polypeptide calcium chelate microcapsules, said method comprising the following steps:

[0021] (1) Preparation of sheep bone powder: Take fresh sheep bones, wash them after crushing, cook at 121°C under high pressure for 40 minutes, then take them out, and dry them at 50°C until the moisture content is less than 6%. The dried sheep bones are pulverized by a grinder and passed through a 100-mesh sieve for later use;

[0022] (2) Preparation of collagen polypeptide: Soak the sheep bone powder prepared in step (1) with 5% acetic acid for 48 hours, take the soaked sheep bone powder and add it to clean water to prepare a 2% mass concentration Sheep bone meal substrate solution, adjust the pH value to 2.0, add pepsin according to the weight ratio of enzyme and substrate solution at 1:100, enzymolyze at 55°C for 3 hours, and put the reaction solution after enzymolysis at 100°C Inactivate the enzyme in a water b...

Embodiment 2

[0035] (1) Preparation of sheep bone powder: Take fresh sheep bones, wash them after breaking, and then repeatedly boil at 100°C to degrease for 3 hours, then make sheep bone paste by ultrafine grinding, then take them out, and dry them at 60°C When the moisture content is less than 6%, the dried sheep bones are pulverized by a grinder and passed through a 100-mesh sieve for later use;

[0036] (2) Preparation of collagen polypeptide: Soak the sheep bone powder prepared in step (1) with 5% acetic acid for 48 hours, take the soaked sheep bone powder and add it to clean water to prepare a 2% mass concentration Sheep bone meal substrate solution, adjust the pH value to 2.0, add pepsin according to the weight ratio of enzyme and substrate solution at 1:100, enzymolyze at 55°C for 3 hours, and put the reaction solution after enzymolysis at 100°C Inactivate the enzyme in a water bath for 5 minutes, then adjust the pH value to 5.5, add papain according to the ratio of enzyme to subst...

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Abstract

The invention relates to a method for preparing low-molecular-weight sheep bone collagen polypeptide calcium chelate microcapsules, which comprises the following steps of: 1, preparing sheep bone meal, namely grinding fresh sheep bones, degreasing, drying, and grinding to prepare sheep bone paste; 2, performing enzymolysis on the sheep bone paste to prepare bone collagen polypeptides; 3, separating and extracting by using an ultrafiltration membrane to obtain low-molecular-weight bone collagen polypeptides; 4, chelating the low-molecular-weight bone collagen polypeptides and a calcium source to prepare low-molecular-weight collagen polypeptide calcium chelates; and 5, preparing the low-molecular-weight sheep bone collagen polypeptide calcium chelate microcapsules. By the method, the addedvalue of sheep bone resources is improved, the comprehensive development and utilization of collagen and bone calcium in pig bones is realized, and the prepared microcapsule calcium supplement has high solubility in water, and has the highest solubility in a range close to human body temperature; and the microcapsule product can cover the peculiar smell of calcium salt.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a low molecular weight sheep bone collagen polypeptide [0002] A preparation method of calcium chelate microcapsules. Background technique [0003] Bone is mainly composed of collagen network structure and calcium salts adhered in it, and the ratio of calcium to phosphorus is 2:1, which is very close to the optimal ratio of calcium absorption by the human body, and is an ideal natural calcium source. my country is a large animal husbandry country. With the development of my country's livestock and poultry breeding industry, nearly 15 million tons of livestock and poultry bones are produced in my country every year. The comprehensive development of livestock and poultry bone resources directly affects the economic benefits of livestock and poultry breeding; the processing and utilization of bones will produce great economic and social benefits, and has great development prospects. ...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K38/39C12P21/06A61P3/02
Inventor 刘丽莉任广跃段续康怀彬唐浩国张慧芸陈俊亮赵胜娟
Owner HENAN UNIV OF SCI & TECH
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