Preparation method of oceanic ossein peptide calcium chelate biological calcium supplement

A technology of collagen peptide calcium and collagen peptide, applied in food preparation, food science, application and other directions, can solve the problems of no chelate, unacceptable land animal food, etc., to achieve safe source, easy absorption and utilization, avoidance of The effect of restrictions on lifestyle

Active Publication Date: 2013-01-16
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The second is that for religious reasons, people in many areas cannot accept food from land animals, etc.
[0013] At present,

Method used

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  • Preparation method of oceanic ossein peptide calcium chelate biological calcium supplement

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Raw material pretreatment: Weigh 1.0kg of fresh salmon bones and wash them repeatedly with clean water. Wash and mince, add 7 times the volume of alkaline water (pH=9), and emulsify at 50°C.

[0049] Enzymolysis: transfer the emulsified raw materials into an enzymolysis tank, heat to 55°C, and adjust the pH to 8.5. Add 2.2g alkaline protease and mix well. Under the conditions of 55°C and pH=8.5, the enzyme hydrolysis was carried out for 3 hours. Then the temperature was lowered to 40° C., the pH was adjusted to 6.5, 5.0 g of papain was added and mixed evenly. The temperature was adjusted to 60°C and reacted for 2h. Inactivate the enzyme at 100°C for 5 minutes.

[0050] Spray drying: the fish bone collagen peptide clarified liquid is evaporated and concentrated to a solid content of 30-50%. Fish collagen peptides were dried using a centrifugal spray dryer. Obtain light yellow powdery marine collagen peptide.

[0051] Configuration of the peptide solution: Weigh 4....

Embodiment 2

[0057] Raw material pretreatment: Weigh 2.0kg of tuna bones and wash them repeatedly with clean water. Wash and mince, add 7 times the volume of alkaline water (pH=9), and emulsify at 50°C.

[0058] Enzymolysis: transfer the emulsified raw materials into an enzymolysis tank, heat to 55°C, and adjust the pH to 8.5. Add 5.0g alkaline protease and mix well. Under the conditions of 55°C and pH=8.5, the enzyme hydrolysis was carried out for 3 hours. Then the temperature was lowered to 40° C., the pH was adjusted to 6.5, 10.0 g of papain was added, and the mixture was evenly mixed. The temperature was adjusted to 60°C and reacted for 2h. Inactivate the enzyme at 100°C for 5 minutes.

[0059] Calcium ion chelation: Weigh 1.5g of calcium hydroxide solid, dissolve it in the polypeptide solution, and make the concentration reach 1.5% by weight. , stir to dissolve. Hydrochloric acid was added to adjust the pH to 6, and the reaction solution was placed in a reaction tank at 60°C for...

Embodiment 3

[0063] The marine collagen chelated calcium powder prepared in Example 1 was scanned by a Fourier transform infrared spectrometer.

[0064] The detection and analysis results of the marine bone collagen peptide calcium chelate by Fourier transform infrared spectroscopy, the analysis of Fourier transform infrared spectroscopy shows that: 3066mc -1 Absorption peak appears at (OH and NH stretching vibration frequencies overlap), at the same time at 925mc -1 There is an absorption peak in one place, indicating that it contains -COOH structure; 1315mc -1 , an absorption peak appears (metaphosphate PO 2 anti-symmetric telescoping); 1085mc -1 A strong absorption peak appears. Chelate Fourier Transform Infrared Spectrum 3066mc -1 Absorption peak narrows at 925mc -1 The absorption peak disappears, indicating that calcium ions bind to the marine collagen peptide at the -COOH position. 3200-3000mc -1 There is a broad absorption peak, and after chelation, the absorption peak here b...

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Abstract

A preparation method of an oceanic ossein peptide calcium chelate biological calcium supplement includes the steps of enzymolysis, chelation and precipitation. Enzymolysis includes adding alkaline protease in fish bones for enzymolysis, and adding papain for enzymolysis to obtain ossein peptide. Chelation includes adding calcium source into ossein peptide solution for chelation reaction. Precipitation includes precipitating chelation liquid with anhydrous ethanol to obtain oceanic ossein peptide calcium chelate. Waste fish bones of oceanic fish product processing are used as raw material to prepare faint yellow powdered fish ossein peptide without fishy smell, and the fish ossein peptide is chelated with calcium chloride. The oceanic ossein peptide calcium chelate is dissolved into faint yellow liquid under meta-acid conditions. Structural identification of the chelate with Fourier transform infrared spectrum shows that calcium ions are combined with -COOH and amino in polypeptide to form coordinate bonds, and accordingly a novel oceanic ossein peptide calcium chelate is generated. Waste fish bones of oceanic fish product processing are used as raw material to prepare ossein peptide, resources are fully utilized, raw material is safe and nontoxic, no harmful substances are participated and generated in the preparation, process is simple, cost is low, and the oceanic ossein peptide calcium chelate is applicable to preparation of health care products and drugs for calcium supplement.

Description

technical field [0001] The invention relates to a preparation method of a marine collagen peptide calcium chelate biological calcium supplement, which belongs to the field of modern food processing and biotechnology. Background technique [0002] Calcium is a mineral element necessary for human life activities and the source of body metabolism. Calcium is also the most abundant mineral element in the human body, and plays an irreplaceable role in maintaining the normal physiological functions of the human body's circulation, breathing, endocrine and bone systems. The dietary composition of Chinese residents is mainly plant food, which contains high content of phytate, cellulose, oxalic acid, etc., and the calcium absorption rate of residents is low, especially the phenomenon of calcium deficiency in pregnant women, the elderly, and children is more serious. Calcium deficiency in the human body can lead to serious consequences such as osteoporosis, rickets, and susceptibilit...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23L33/00
Inventor 蔡木易鲁军高菲谷瑞增易维学董哲马勇陈亮潘兴昌徐亚光马永庆林峰金振涛刘文颖陆路刘艳魏颖张海欣
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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