Application of melia azedarach leaf extract in whitening product

A technology of neem leaves and extracts is applied in the application field of neem leaf extracts in whitening products, and achieves the effects of whitening development and application value, inhibitory activity, and good development and application value.

Inactive Publication Date: 2017-01-25
广东真丽斯化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Neem leaves have the functions of regulating qi and removing dampness, detoxifying and killing insects. The extracts of neem leaves have been widely used as botanical pesticides and integrated pest management, but there are few reports about the active ingredients in neem leaves used in whitening products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preparation method of neem leaf extract is as follows: the neem leaf is pulverized, placed in supercritical CO 2 In the extraction device, the temperature of the extraction tank is controlled to be 50°C, the pressure is 40Mpa, the temperature of the first separation tank is 40°C, the pressure is 5Mpa, the temperature of the second separation tank is 35°C, the pressure is 4Mpa, CO 2 The flow rate is 32L / h, extract for 2 hours to obtain medicinal residues, add 75% ethanol to the medicinal residues, the volume mass ratio of ethanol and medicinal residues is 8mL: 1g, extract twice at 60°C for 1 hour each time, combine the leaching The extract was concentrated under reduced pressure at 70°C and 0.2 bar, and freeze-dried to obtain the obtained product.

Embodiment 2

[0018] Neem leaf extract inhibits tyrosinase activity experiment:

[0019] Take 5 test tubes, wherein No. 1 and No. 2 test tubes add 1 mL of 0.05wt% neem leaf extract solution, No. 3 and No. 4 test tubes add 1 mL of phosphate buffer (pH=6.8) respectively, 1 mL of 0.05 wt% arbutin solution. Add 0.5 mL of tyrosinase solution (enzyme activity unit 125u / mL) to No. 1, No. 3 and No. 5 test tubes, add 0.5 mL of phosphate buffer (pH=6.8) to No. 2 and No. 4 test tubes, and 5 Shake the test tubes evenly, put them in a water bath at 35°C for 10 minutes, add 2 mL of 0.05 wt% tyrosine solution to it, react for 10 minutes, measure the absorbance T of each test tube at 475 nm, and mark them as T1, T2, T3, T4, T5, And calculate the inhibition rate.

[0020] The calculation formula of inhibition rate is: I(%)=[1-(T1-T2) / (T3-T4)]×100%

[0021] It is calculated that the inhibition rate of 0.05wt% neem leaf extract solution to tyrosinase can reach 81.1%, while the inhibition rate of arbutin as...

Embodiment 3

[0023] Inhibitory effect of neem leaf extract on melanin:

[0024] The improved method of Hosoi et al. was used to determine the inhibitory effect of neem leaf extract on melanin. B16 melanocytes (purchased from Shanghai Yansheng Industrial Co., Ltd.) were cultured in a 96-well plate at a density of 1 × 105. After 24 hours, the medium was changed, and 0.05 wt% neem leaf extract solution was added. After 72 hours, the cells were incubated with phosphate buffer ( pH = 6.8) and washed twice, the sample was air-dried, dissolved in 200 μL 1mol / L NaOH (containing 1% DMSO), cooled after heating at 80°C to 1h, and read the absorbance value on an enzyme-linked immunosorbent assay with a wavelength of 475nm. Calculate the inhibition rate; use the same method to measure the absorbance of 0.05wt% arbutin and calculate the inhibition rate.

[0025] It is calculated that the inhibition rate of melanin by 0.05wt% neem leaf extract solution can reach 69.8%, while the inhibition rate of arbut...

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PUM

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Abstract

The invention provides application of melia azedarach leaf extract in a whitening product. A preparation method of the melia azedarach leaf extract comprises the following steps: smashing melia azedarach leaves; putting the melia azedarach leaves in a supercritical CO2 extracting device, and extracting for 2 hours, thus obtaining medicine residues; adding 75 percent of alcohol in the medicine residues; leaching twice under 60 DEG C; merging leaching solutions; carrying out vacuum concentration and freeze drying, thus obtaining the melia azedarach leaf extract. The melia azedarach leaf extract provided by the invention contains whitening active ingredients, is capable of obviously inhibiting the activity of tyrosinase and hindering the formation of melanin, is safe in source, and has very good whitening development application value.

Description

technical field [0001] The invention relates to the technical field of natural cosmetics, in particular to the application of neem leaf extract in whitening products. Background technique [0002] In recent years, due to the increasing pressure of life and work and the aggravation of environmental pollution, people are facing more and more skin problems. Spots and pigmentation caused by various reasons have become common problems. Whitening and antioxidant products Has become a mainstream variety. As people's consumption concepts become increasingly mature, consumers pay more attention to whitening products not only in their efficacy but also in their safety. [0003] There are many reasons for skin spots and pigmentation, such as genetic factors and environmental factors such as ultraviolet rays, exhaust gas and active oxygen. According to current research results, melanin is mainly produced by melanocytes in the basal layer of human skin epidermis, and its content and di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/02
CPCA61K8/97A61K2800/782A61K2800/805A61Q19/02
Inventor 黄楚明黄楚佳黄楚杰
Owner 广东真丽斯化妆品有限公司
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