173results about How to "Source security" patented technology

The invention relates to a lactobacillus plantarum strain having functions of effectively degrading nitrite and strongly producing acid and an application of the lactobacillus plantarum strain, and belongs to the technical field of bioengineering. The lactobacillus plantarum strain having the functions of effectively degrading nitrite and strongly producing acid is selected from conventional fermented pickled vegetables in Shenyang peasant families, wherein the strain is named FM-LP1-1, identified to be lactobacillus plantarum, and the strain is preserved in China General Microbiological Culture Collection Center with the preservation number of CGMCC No. 9488 on August 4, 2014. By taking FM-LP1-1 as a fermenting agent for fermenting vegetables, the content of nitrate in the fermented vegetable products is effectively reduced, the fermenting duration of the vegetables is shortened and the fermenting flavor of the vegetables is enhanced; and the strain disclosed by the invention is broad in application prospect.

The invention discloses a cosmetic containing oceanic shellfish active peptide and a preparation method and application thereof. The cosmetic contains 0.1 to 10 percent of oceanic shellfish active peptide, or 0.1 to 10 percent of oceanic shellfish active peptide and 1 to 10 percent of hyaluronic acid, or 0.1 to 10 percent of oceanic shellfish active peptide, 1 to 10 percent of hyaluronic acid and1 to 25 percent of vitamin C (VC), and the balance is cosmetic matrix components. The peptide is formed by boiling, ultrasonic, enzymolysis and ultra-filtration of oceanic shellfish meat; and besidesthe active peptide, the oceanic shellfish meat also contains free amino acid, glycosaminoglycan, polyunsaturated fatty acid, vitamins, trace elements and the like. The problems of application and thelike of the oceanic shellfish active peptide in the cosmetic are solved; and the prepared cream, emulsion, aqueous agent, gel, aerosol or mask has more complete and remarkable skin-care or hair-care effect. After the hyaluronic acid and the VC are added, the effects of preserving moisture, increasing the elasticity and the smoothness of the skin, removing flaws and improving the skin color are remarkably enhanced.

The invention discloses a secondary aluminium cell and a preparation method thereof. Foamed aluminium serves as a cathode. A carbon and sulfur composite material or sulfurized polyacrylonitrile serves as an anode active material. A halogen aluminate ionic liquid formed from halogen aluminium acid and any one of halogenated quaternary ammonium salt, quaternary alkylphosphonium salt or quaternary sulfosalt is obtained by preparing electrolyte. The cell has the advantages of high energy density, good cycle performance, safety, environmental protection, no pollution in the preparation process, low cost and simple process.

The invention discloses a rechargeable aluminum battery and a preparation method thereof. The rechargeable aluminum battery is prepared by selecting metal aluminum or an aluminum alloy, preferable foamed aluminum, as a cathode, taking vulcanized polyvinyl chloride or a carbon-sulfur compound material as an anode active substance and taking aluminic acid halide ionic liquid formed by aluminum halide and any one of quaternary ammonium salt or quaternary microcosmic salt or quaternary sulfosalt a electrolyte. The rechargeable aluminum battery has the advantages of high battery energy density, good circulation performance, safety, environmental protection, pollution-free preparation process, low cost, simple process and vast application prospect.

The invention discloses a rechargeable aluminum-sulfur battery and a preparation method thereof. The rechargeable aluminum-sulfur battery is prepared by adopting metallic aluminum or aluminum alloy, preferably foamed aluminum as cathode, polymeric organosulfides as anode active substances, and halogenated aluminic acid ionic liquid formed by halogenated aluminum with any one of quaternary ammonium salt, quaternary phosphor salt or quaternary sulfosalt as electrolyte. The rechargeable aluminum-sulfur battery has high energy density, good circulation performance, safety and environmental protection, no pollution in preparation process, low cost and simple technique, thus having good application prospect.

The invention discloses an aluminium polymer secondary battery and a preparation method thereof. The aluminium polymer secondary battery is prepared by taking metallic aluminium or aluminium alloy, preferably foamed aluminium as the negative pole, vulcanized polyvinyl chloride or carbon-sulfur composite materials as the positive active materials, and halogen aluminic acid ionic liquid formed by aluminum subhalide and one of hyamine, phosphonium actericideor, or sulfur actericide as the electrolyte solution. The battery has the advantages of high energy density, good cycle performance, environment protection, no pollution during the preparation, low cost, simple process and favorable application prospect.

The present invention relates to a suspension emulsion gel loaded with fat-soluble vitamins and a preparation method thereof, and belongs to the technical field of food processing. The suspension emulsion gel comprises the following components in parts by weight: 1-10 parts of fat-soluble vitamins, 1-10 parts of vegetable proteins, 0.5-3 parts of polysaccharides and 30-60 parts of vegetable oil rich in unsaturated fatty acids. The preparation raw materials are cheap in source, safe, good in biocompatibility, fast in biodegradation and easy for modification. The prepared suspension emulsion gelhas good stability, is simple in technology, low in cots, safe, environmentally protective, good in product stability and high in bioavailability, can be used in various foods to enhance sensory value, edible value and nutritional value, and can also be used for preparing healthy functional foods or healthy accessory ingredients.

The invention relates to a medical adhesive or hemostatic, which comprises aminated gelatin aqueous solution and aldehydic polysaccharide aqueous solution, wherein a method for preparing the aminated gelatin aqueous solution comprises the following steps of: adding gelatin, ethylenediamine and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide in a mass ratio of (2-20):(1-10):(1-5) into water for reaction, dialyzing, freezing and drying to obtain the aminated gelatin aqueous solution; and a method for preparing the aldehydic polysaccharide aqueous solution comprises the following steps of: mixing polysaccharide or starch aqueous solution and NaIO4 aqueous solution in a volume ratio of (1-2):(1-2), reacting at room temperature, dialyzing, freezing and drying to obtain the aldehydic polysaccharide aqueous solution. In the medical adhesive or hemostatic, the coagulation time is shorter than that of fibrin glue, and the bonding strength is higher than that of the fibrin glue; the fibrin glue bonds incisions on backs of rats with the length of 2cm successfully, and is used for the hemostasis of incisions of livers of the rats; and a preparation process for aminated gelatin and aldehydic polysaccharide is simple and low in cost.

The invention discloses a method for preparing lycium ruthenicum pigment microcapsules by using a piercing method. The method comprises the following steps of using lycium ruthenicum fresh fruits or lycium ruthenicum dried fruits to serve as raw materials, crushing, conducting acidity ethanol digestion, extracting by using a microwave extractor, extracting residues for 2-3 times by using the acidity ethanol after filtering, combining an extracting solution, and conducting decompression concentration to obtain a pigment concentrated solution; and conducting adsorption, elution, desorption and decompression concentration on the pigment concentrated solution through macroporous resin, adding an embedding medium in proportion, conducting solidification, filtering, laminating, filtering and return soaking, drying under ordinary pressure at the temperature of 40-50 DEG C to obtain the lycium ruthenicum pigment microcapsules. An embedding ratio of the lycium ruthenicum pigment microcapsules prepared by the process is 70%-80%, lycium ruthenicum pigments have a slow release function after microencapsulation, more than 70% pigments can be released within 24 hours, the shape is kept to be complete, stability of the lycium ruthenicum pigment microcapsules in an 75% of relative humidity environment is obviously improved, and the weight increase of the microcapsules caused by moisture absorption is lower than 10%.

The invention discloses micro-capsule preparation and a method for preparing the same, belonging to the microorganism field and the preparation field. The micro-capsule preparation uses sodium alginate as capsule material solution and CaCl2 as curing agent and contains Enterococcus Faecium the preservation number of which is CGMCC No.2516. The invention has the advantages that: the pre-embedding and after fermentation method is adopted, thereby thalli can be proliferated continuously under the micro-capsule microenvironment; the size of the cell density is controllable; the density of the micro-capsule thalli higher than that of the after embedding method can be reached; the pre-embedding method greatly reduces the utilization amount of cells during the encapsulization process, avoids the adverse factors of low encapsulization efficiency and productive rate due to impact of the cell density in the after embedding process, and more easily obtains encapsulated products with better shape and stability.

The invention relates to a brown sour milk beverage containing real fruit variants. The beverage is prepared by the following steps of: taking dried skim milk, glucose, white sugar, concentrated lemon juice, orange variants, pectin, guar gum, xanthan gum and edible essence as main raw materials, and fermenting lactobacillus casei. In the preparation method, orange variants and the brown sour milk beverage are effectively combined, and stabilizer compound combination is together used to ensure the quality of the final product, thus the brown sour milk beverage has the advantages of excellent steadiness and long quality guarantee period.

The invention relates to a stem cell repairing material as well as a preparation method and application thereof. The stem cell repairing material comprises a stem cell and a cell carrier, wherein the stem cell is an autologous adipose-derived mesenchymal stem cell (ADSC) with a concentration of 105-108 / ml, and the cell carrier is autoserum, normal saline (0.9%) or a glucose injection (5%). In the invention, adopted seed cells are prepared through separating and purifying autologous adipose tissues of patients, thereby avoiding the ethical disputes and the immunological rejection. The material drawing of the adipose tissues can be performed by using an instrument suction method, which is simple in operation and can bring less traumas and pains to the patients. The cell carrier used in the invention is the autoserum, normal saline (0.9%) or glucose injection (5%), therefore, the cell carrier mixed with mesenchymal stem cells and other ingredients can be injected into the patents by virtue of syringes. By using the stem cell repairing material provided by the invention, the operation process is simple, no scar is left, and the wounds and pains for the patients are avoided, therefore, the stem cell repairing material can be easily accepted by the patients.

The invention relates to the technical field of medical instruments, and in particular relates to an aquatic product fish skin gelatin sponge and a preparation method thereof. The aquatic product fish skin gelatin is used as a main material, chitosan is used as an auxiliary material, and glutaraldehyde is used as a cross-linking agent; the raw material liquids of different proportions are subjected to freeze drying to prepare fish skin gelatin sponges with different properties; the preparation process of the sponge is simple; the sponge is low in cost and readily available; some defects of the conventional collagen sponge are overcome. The prepared fish skin gelatin sponge has a uniform porous netlike microstructure and higher mechanical strength and good moisture absorption and is a better biomedical material.

The invention discloses a preparation method and application of a cinnamaldehyde submicroemulsion for injection. The cinnamaldehyde submicroemulsion is prepared from the following components in percentage by weight-volume: 5.1-30.6 percent of oil, 0.5-3.0 percent of emulsifier, 1-4 percent of osmotic pressure regulator, 0.1-0.3 percent of stabilizer, 0.05-0.2 percent of antioxidant and the balance of distilled water. The submicroemulsion has excellent stability, biocompatibility and safety; the preparation method is simple, the quality is controllable, and industrial production is possible; the submicroemulsion can be used for treating tumors and other diseases.

The invention discloses a method for improving storage stability of fruit wine. The method comprises the following steps: adding gelatin and tannin into the fruit wine to perform clarification, adding lactic acid bacteria extracellular polysaccharide therein, uniformly stirring and standing for 3-5 days and filtrating, wherein the lactic acid bacteria extracellular polysaccharide is prepared by fermentation of Lactobacillius plantarum CGMCC No. 10178. Compared with the prior art, the method disclosed the invention is safe, harmless, simple and effective and is capable of obviously improving the storage stability of fruit wine, so as to provide reliable strain resources to fruit wine processing and manufacturing as well as provide technical support to healthy development of the fruit processing industry.

The invention discloses lactobacillus rhamnosus and application thereof in preparing bacteriocin. The preservation taxonomic name of lactobacillus rhamnosus is lactobacillus rhamnosus zrx01, the lactobacillus rhamnosus is preserved in the China General Microbiological Culture Collection Center on December 22nd, 2017, and the preservation number of lactobacillus rhamnosus is CGMCC No.15076. The bacteriocin generated by the screened lactobacillus rhamnosus zrx01 can inhibit various harmful bacteria, especially has a strong inhibition function on gram-positive staphylococcus aureus, Listeria monocytogenes, bacillus anthraci, gram-negative escherichia coli, campylobacter jejuni and acid soil alicyclobacillus spp, has no inhibition function on lactobacillus plantarum, lactobacillus acidophilusand other probiotics, and also has no inhibition function on lactobacillus rhamnosus zrx01 and most bacteriocin.

The present invention discloses the composition of antioxidant of bamboo leaves (AOB) and its use. The purpose of present invention is to provide a new food additive which is natural, nutritional, and muti-functional, and which is of rich resources, safety, good effect, and low cost. AOB is yellow or brown powders or particles obtained from bamboo leaves, wherein the main antioxidative components include flavones, lactones, phenolic acids. AOB can either inhibit lipid autoxidation chain reaction, or chelate transitional metal ions, and can be used as primary and second antioxidant. AOB can eliminate nitrite and inhibit the synthesis of N-nitrosamine, and has anti-bacteria, bacteriostatic, deodorizing, aroma enhancing etc. functions. AOB can be commonly used in oil-containing food, meat product, fishery product, expanded food etc. food systems.

The invention relates to a method for preparing biodegradable super water absorbent gamma-polyglutamic acid (gamma-PGA) hydrogel. The method comprises the following steps: using transglutaminase (TG enzyme) as a crosslinking agent, and through acyl transfer reaction, performing crosslinking on gamma-PGA and casein protein to form composite protein super water absorbent hydrogel. The invention further relates to the biodegradable super water absorbent gamma-polyglutamic acid (gamma-PGA) hydrogel and application thereof. The method has the advantages that (1) the reaction conditions are mild, the time is short, the operation is simple and convenient, and requirements on equipment are low; (2) sources of raw materials are security, and the hydrogel is environmental-friendly and degradable; (3) lots of active sites are positioned inside the hydrogel, and can be combined with medicine to retain the activity of the medicine, so that an ideal biomedical material is provided; (4) the hydrogel has a stronger water absorption swelling property and a stable watering binding capacity.

The invention relates to the field of biological medicine, in particular to a molecular weight grading and purifying method of polyhydroxyalkanoate, which comprises the following steps: S1, dissolving a polyhydroxyalkanoate raw material in a good solvent, and adding a catalyst for heating degradation; S2, cooling the solution heated and degraded in the step S1 to room temperature, conducting mixing with a poor solvent, precipitating and separating out a degradation product, and collecting the degradation product after sedimentation; and S3, centrifuging the degradation product collected in the step S2, and washing and drying the degradation product to finish molecular weight grading and purification of the polyhydroxyalkanoate. The method is simple in step and convenient to operate, molecular weight grading can be carried out on polyhydroxyalkanoate while the polyhydroxyalkanoate is purified, and the polyhydroxyalkanoate obtained after grading and purification through the method is high in purity, uniform and accurate in molecular weight and narrow in molecular weight distribution and has a higher active terminal.

A pipe source of UV flux has an inner pipe made of UV transmissive material and coated on its outer surface with a UV emitting phosphor. An outer pipe has a cathode array disposed on or near its inner surface, such as an array of thermionic filament cathodes mounted longitudinally or transverse to the length of the pipe, cold cathode arrays formed on the inner surface of the pipe or cold cathode arrays formed on separate substrates which are then attached to the inner surface of the outer pipe. The ends of this two-pipe assembly are hermetically sealed with flanges or end plates at either end of the pipe and evacuated to a pressure below 1×10−3 Torr. Internal spacing rings may be used to provide additional separation between the inner and out pipes. Current from the cathode arrays is accelerated by an anode voltage to strike the UV phosphors when then emit UV light flux which illuminates the inside of the pipe and the fluid material flowing through the inner pipe.

The invention discloses a traditional Chinese medicinal moistening sugar-free drink and a preparation method thereof and belongs to the field of production of drinks. The traditional Chinese medicinal moistening sugar-free drink is prepared from raw materials of water, mesona chinensis benth, grosvener siraitia, lalang grass rhizome, chrysanthemum, spike, honeysuckle and liquorice. The drink is prepared by the following main steps of: weighing the mesona chinensis benth, the grosvener siraitia, the lalang grass rhizome, the chrysanthemum, the spike, the honeysuckle and the liquorice, adding water for decoction, keeping the slight boiling state for 30 to 150 minutes and filtering; adding water into filter liquor to reach the water amount before decoction, filtering to obtain filter liquor, and filling and sterilizing the filter liquor, wherein the filter liquor also can be concentrated into extract, is diluted by water, added with water to reach the water amount before decoction and is filled and sterilized. The traditional Chinese medicinal moistening sugar-free drink does not contain any sugar substitute or artificial sweetener, can clear heat and relieve summer heat in spring and summer and nourish Yin and moisten dryness in autumn and winter, and is particularly suitable for people with special requirements on sugar intake.

The invention discloses application of vibration stimulation in regulation of in vitro osteogenesis and adipogenic differentiation of bone marrow-derived mesenchymal stem cells and belongs to the technical field of cell biotechnology and bone tissue engineering. Osteogenesis differentiation culture and adipogenic differentiation culture are respectively carried out on bone marrow-derived mesenchymal stem cells of people in vitro, vibration stimulation is carried out once a day and every time is 20-45 minutes, and the in vitro osteogenesis differentiation and adipogenic differentiation of bone marrow-derived mesenchymal stem cells are facilitated to be promoted. Vibration stimulation is applied to the bone marrow-derived mesenchymal stem cells, formation of calcium of extracellular matrix and expression of osteogenesis differentiation marker genes of RUNX2 and COL1A1 in mRNA level can be upwards regulated, generation of lipid of epimatrix and expression of adipogenic differentiation marker genes of PPARG2 and CEBPA mRNA can be restrained, and a safe and efficient seed cell source is provided for promoting stem cell osteogenesis differentiation to generate more functional osteoblasts.

This invention exposed the production method of angiotensin-converting enzyme inhibitory petides. The steps are as follow: 1) mix the rice dregs and distiller in container by the proportion of 1:4 - 4.5, then adjust the pH value to 7.9 - 8.1 with alkali solutions of 2.0 - 3.0M density; 2) add trypsin in container then put it in constant temperature water bath to react; the temperature of water bath is 35 - 40DEG C, the time of reaction is 3.5 - 4.5 hours. The amount of trypsin is as much as 1.4 - 1.6% of the weight of rice dregs; 3) then put the container in boiling water to stop the reaction; separating the obtained product by centrifugal, then condensing, freezing and drying the supernatant, what obtained is angiotensin-converting enzyme inhibitory petides. This finally obtained angiotensin-converting enzyme inhibitory petides is safe, economy and effective.

The invention provides a tobacco stem field stacking and retting fermentation method. After tobaccos are matured and harvested, tobacco stems are collected on the spot and are broken according to a certain size; and then stacking and retting fermentation is performed on the tobacco stems by mixing the tobacco stems with water, soil, cow dung, phosphatic fertilizer, a ferment activator and lime according to proper proportions. The invention also provides a compost product produced by the tobacco stem field stacking and retting fermentation method. A using method for the compost product obtained by tobacco stem stacking and retting fermentation is characterized in that the compost product is fertilized every two crop planting periods preferably; the fermented compost product is used as a base fertilizer; and a ditch is formed, and the compost product is fertilized into the deep ditch. According to the tobacco stem field stacking and retting fermentation method, the application amount of chemical fertilizers can be reduced, a soil ecological environment is protected, the soil is improved, the soil fertility is improved, and the tobacco stem field stacking and retting fermentation method is safe and ecological. By the method, raw materials are readily available, convenient to collect, easy to operate, environmentally-friendly, sanitary and low in cost, and tobacco farmers can easily master the method; the cost is low, the expense is low, the production technology is simple ad can be easily popularized and applied in a large tobacco area; the quality of a product is stable; and the economical and ecological benefits are outstanding.

The invention provides application of melia azedarach leaf extract in a whitening product. A preparation method of the melia azedarach leaf extract comprises the following steps: smashing melia azedarach leaves; putting the melia azedarach leaves in a supercritical CO2 extracting device, and extracting for 2 hours, thus obtaining medicine residues; adding 75 percent of alcohol in the medicine residues; leaching twice under 60 DEG C; merging leaching solutions; carrying out vacuum concentration and freeze drying, thus obtaining the melia azedarach leaf extract. The melia azedarach leaf extract provided by the invention contains whitening active ingredients, is capable of obviously inhibiting the activity of tyrosinase and hindering the formation of melanin, is safe in source, and has very good whitening development application value.

The invention provides a garlic-specified fertilizer capable of increasing content of alliin. The garlic-specified fertilizer is prepared from the following effective components in parts by weight: 0.01 part of EM bacteria, 0.3-0.7 part of polyglutamic acid, 3-6 parts of humic acid, 20-24 parts of N, 5-10 parts of P2O5, 14-17 parts of K2O, 0.1-0.3 part of Fe, 0.2-0.5 part of Zn, 0.2-0.3 part of B and 0.1-0.5 part of Cu, wherein N is commonly provided by urea and monoammonium phosphate; P2O5 is provided by monoammonium phosphate; K2O is provided by potassium sulfate; Fe is provided by ferrous sulfate; Zn is provided by zinc sulfate; B is provided by borax; Cu is provided by copper sulfate. The garlic-specified fertilizer is capable of improving the growth condition of the garlic, increasing the content of alliin in the garlic, increasing the fertilizer utilization efficiency, increasing the yield of the garlic and improving the quality of the garlic.

The invention discloses a method for producing PUFA (polyunsaturated fatty acid) by utilizing potato in an Aspergillus niger xj strain. The method comprises the following steps of inoculating the Aspergillus niger xj strain in a PDA (potato dextrose agar) solid agarslantculture-medium to culture for three days at 27 DEG C and activate for three times, inoculating in a PDA liquid seed culture medium again to culture in a shake flask for three days at 27 DEG C and150rpm to obtain a seed liquid, transplanting the seed liquid in a 13L PDA fermentation medium according to a proportion of 5%(v / v) at 28+ / -1 DEG C and a stirring speed of 200-300rpm, controlling the ventilation amount within 1-2L / min, culturing for five days and stopping fermenting; and filtering through four layers of sterile gauze to remove mycelium, and collecting fermentation liquid to obtain the PUFA. According to the method for producing the PUFA, disclosed by the invention, the content of the obtained unsaturated fatty acid is high, the culture medium is easy to obtain, the fermentation conditions are stable, and the process is simple.

The invention discloses an all-plant beverage free of edible sugar and chemical additives and a production method thereof. The all-plant beverage is produced by of 5000-10000 parts of pure water, 5-50 parts of stevia rebaudiana leaves, 2-20 parts of siraitia grosvenori, 2-20 parts of hippophae rhamnoides leaves, 2-20 parts of roses, 2-20 parts of jasmine flowers, 2-20 parts of chrysanthemums, 2-20 parts of lotus leaves, 3-30 parts of lotus seeds, 3-30 parts of mulberry leaves, 2-20 parts of kudzu vine roots, 2-20 parts of ganoderma lucidum, 3-30 parts of mesona chinensis, 2-20 parts of honeysuckles, 2-20 parts of licorice roots, 2-20 parts of Chinese wolfberry fruits and 2-20 parts of cassia seeds. After the raw materials are subjected to ultrasonic cleaning, the roses, the jasmine flowers, the chrysanthemums, the hippophae rhamnoides leaves and the Chinese wolfberry fruits are directly decocted to obtain liquid A, the stevia rebaudiana leaves, the siraitia grosvenori, the lotus leaves, the lotus seeds, the kudzu vine roots, the ganoderma lucidum, the mesona chinensis, the honeysuckles, the licorice roots and the cassia seeds are graded and smashed and then are decocted to obtain liquid B, and the liquid A and the liquid B are mixed and then are processed to obtain the all-plant beverage. The beverage does not contain the edible sugar, essence and the chemical additives, contains beneficial ingredients, such as stevioside, flavone, vitamins and coarse fiber, is suitable for daily drinking of people and is especially suitable for diabetic patients to drink.

The invention provides a method for inactivating listeria monocytogenes on a preservative film. The method comprises the following steps of taking visible light from a bromine tungsten lamp as a light source, and utilizing a photosensitizer to inactivate the listeria monocytogenes on the preservative film. The method disclosed by the invention has the advantages that (1) a used photosensitizer has the characteristics of better photosensitive activity, wider wavelength of applicable visible light, increment of action depth, higher speed of internal secretion of an organism and increment of ROS (Reactive oxygen species) production; microorganisms on the surface of the preservative film can be effectively inactivated after the photosensitization of the photosensitizer, and a mediated photodynamic sterilization technology can be taken as a safe replacement method for a traditional sterilization technology; (2) the method is a novel, non-thermal, eco-friendly, economic and effective sterilization technology, is also a method for non-specifically inactivating the microorganisms on the surface of the preservative film, and has broad-spectrum sterilization characteristics; (3) the involved photodynamic sterilization technology is used for inactivating the microorganisms on the surface of the preservative film while the excellent flavor of food is furthest kept, the mouthfeel of the food is better, and the shelf life is effectively prolonged.