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Method for inactivating listeria monocytogenes on preservative film

A technology of Listeria monocytogenes and plastic wrap, which is applied in the field of inactivation of Listeria monocytogenes on plastic wrap, can solve the problems of no food plastic wrap, etc., and achieve good sterilization effect, low cost, and wide source of raw materials Effect

Inactive Publication Date: 2016-06-01
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The non-thermal photodynamic sterilization technology developed by using visible light as the excitation light source is not only used in the medical field, but also used in the inactivation of bacteria and viruses, but there is no report on the application of food preservation film

Method used

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  • Method for inactivating listeria monocytogenes on preservative film
  • Method for inactivating listeria monocytogenes on preservative film
  • Method for inactivating listeria monocytogenes on preservative film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Photosensitizer concentration experiment

[0037] Pretreatment: The aseptic cling film sample used for the photosensitivity test is divided into discs with a radius of 2.0 cm each to simplify the experimental process and minimize the lighting area. Each package sample is in 25mL Listeria monocytogenes suspension (1×10 7 CFU / mL) is soaked in water so that bacteria can adhere to the surface of the plastic wrap. To make the bacterial cells adhere better, place the soaked sample in a 6-well cell culture plate for 10 minutes.

[0038] Treatment of photosensitizer concentration: dilute curcumin with PBS, prepare curcumin solutions with final concentrations of 0, 1, 5, 10, 25, 50, and 75 mg / L, and prepare for use; then, the liquid with Listeria monocytogenes adhered The packaged samples were incubated with 1-75mg / L curcumin solution for 10 minutes under dark conditions. The blank sample was incubated with PBS buffer for the same length of time. After incubating with the curcumin...

Embodiment 2

[0041] Light time test

[0042] Pretreatment: The aseptic cling film sample used for the photosensitivity test is divided into discs with a radius of 2.0 cm each to simplify the experimental process and minimize the lighting area. Each package sample is in 25mL Listeria monocytogenes suspension (1×10 7 CFU / mL) is soaked in water so that bacteria can adhere to the surface of the plastic wrap. To make the bacterial cells adhere better, place the soaked sample in a 6-well cell culture plate for 10 minutes.

[0043] Illumination time treatment: Incubate the package sample with the Listeria monocytogenes bacterial solution in a 10mg / L curcumin solution for 10 minutes under dark conditions. The blank sample was incubated with PBS buffer for the same length of time. After incubating with the curcumin solution, place all the packaged samples in the sample chamber of the bromine tungsten lamp and irradiate them for 0, 5, 10, 20, 30, 45, and 60 minutes. The blank sample does not require l...

Embodiment 3

[0046] Incubation time test

[0047] Pretreatment: The aseptic cling film sample used for the photosensitivity test is divided into discs with a radius of 2.0 cm each to simplify the experimental process and minimize the lighting area. Each package sample is in 25mL Listeria monocytogenes suspension (1×10 7 CFU / mL) is soaked in water so that bacteria can adhere to the surface of the plastic wrap. To make the bacterial cells adhere better, place the soaked sample in a 6-well cell culture plate for 10 minutes.

[0048] Incubation time treatment: Incubate the package sample with the Listeria monocytogenes bacterial solution in a 10mg / L curcumin solution under dark conditions for 0, 1, 5, 10, 15, 20, 30 minutes. The blank sample was incubated with PBS buffer for the same length of time. After incubating with the curcumin solution, place all the packaged samples in the sample chamber of the bromine tungsten lamp and illuminate for 10 minutes. The blank sample does not require light. ...

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Abstract

The invention provides a method for inactivating listeria monocytogenes on a preservative film. The method comprises the following steps of taking visible light from a bromine tungsten lamp as a light source, and utilizing a photosensitizer to inactivate the listeria monocytogenes on the preservative film. The method disclosed by the invention has the advantages that (1) a used photosensitizer has the characteristics of better photosensitive activity, wider wavelength of applicable visible light, increment of action depth, higher speed of internal secretion of an organism and increment of ROS (Reactive oxygen species) production; microorganisms on the surface of the preservative film can be effectively inactivated after the photosensitization of the photosensitizer, and a mediated photodynamic sterilization technology can be taken as a safe replacement method for a traditional sterilization technology; (2) the method is a novel, non-thermal, eco-friendly, economic and effective sterilization technology, is also a method for non-specifically inactivating the microorganisms on the surface of the preservative film, and has broad-spectrum sterilization characteristics; (3) the involved photodynamic sterilization technology is used for inactivating the microorganisms on the surface of the preservative film while the excellent flavor of food is furthest kept, the mouthfeel of the food is better, and the shelf life is effectively prolonged.

Description

Technical field [0001] The invention relates to the technical field of food processing, in particular to a method for inactivating Listeria monocytogenes on a fresh-keeping film. Background technique [0002] As the pathogen of the four major food-borne diseases, Listeria monocytogenes is a zoonotic pathogen. It can sterilize whole milk, fast food, instant food, aquatic products, raw vegetables, Fresh-cut fruits, cooked meat products, cheese, rice, salad dressings, and all raw meats grow in large numbers. The most risky foods are dairy products / cooked meat products / refrigerated meat products. Listeria monocytogenes not only contaminates the food itself, but also often separates from the surface of the plastic wrap, the conveyor belt that carries the food, the storage box, and the hand truck. This is because Listeria monocytogenes tends to contaminate products during packaging, transportation and storage. [0003] At present, the new technologies for controlling the external micro...

Claims

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Application Information

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IPC IPC(8): A61L2/08A61L101/34
CPCA61L2/088A61L2/084
Inventor 林少玲黄志伟崔建婷朱子瑶黄晨楹陈妮王佩瑶孔令华
Owner FUJIAN AGRI & FORESTRY UNIV
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