The invention relates to the technical field of chemical analysis, and discloses a method for extracting, separating and detecting N-
nitrosamine, which comprises the following steps: obtaining a sample to be detected; a
sample purification process; preparing an extraction solution; an extraction solution
separation process; concentrating the extracted solution; performing chromatographic detection and quantitative analysis; the method comprises the following steps: crushing a to-be-detected sample, crushing the to-be-detected sample into 0.01 g of
powder, and mixing the
powder with
sodium chloride,
barium hydroxide and water. According to the invention, by carrying out step-by-step sample treatment procedures on the to-be-detected sample before the detection work, the interference factors of the to-be-detected sample can be effectively reduced, the influence on a detection instrument is reduced, so that the obtained detection result and detection data are more accurate, and in addition, when the method is used for detecting, the detection accuracy is improved. The method does not need to weigh a large number of samples like a traditional detection mode, the whole operation is more convenient, and the detection requirement of the N-
nitrosamine in the smoked and cured food is met.