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Method for measuring tobacco-specific N-nitrosamine in tobacco

A determination method and technology of nitrosamines, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of shortage and good separation effect, achieve good accuracy and precision, improve detection efficiency, and strong chromatographic separation ability Effect

Inactive Publication Date: 2015-02-18
SHANGHAI TOBACCO GRP CO LTD
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  • Abstract
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Problems solved by technology

[0004] In view of the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a kind of assay method of tobacco-specific N-nitrosamine in tobacco, be used for solving the problem in the prior art that the processing process is simple, the separation effect is good, selectivity and Problems with the Determination Method of Tobacco Specific N-Nitrosamines in Tobacco with High Sensitivity

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  • Method for measuring tobacco-specific N-nitrosamine in tobacco
  • Method for measuring tobacco-specific N-nitrosamine in tobacco
  • Method for measuring tobacco-specific N-nitrosamine in tobacco

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Embodiment 1

[0049] 1 Reagents and instruments

[0050] 1.1 Reagents

[0051] Target objects: NNN, NAT, NAB, NNK (0.5mg / ml, AccuStandard company); internal standard: d4-NNN, d4-NAT, d4-NAB, d4-NNK (100ug / mL, AccuStandard company); methanol, di Chloromethane (chromatographically pure, Merck Company); sodium hydroxide (analytical pure, Sinopharm Chemical Reagent Co., Ltd.); anhydrous sodium sulfate (analytical pure, Sinopharm Chemical Reagent Co., Ltd.)

[0052] 1.2 Instruments

[0053] AG104 electronic balance (sensitivity: 0.0001g, Mettler Toledo, Switzerland); 7000B gas chromatography-triple quadrupole tandem mass spectrometer (Agilent Technology Co., Ltd., USA); SW12H ultrasonic instrument (SONO, Switzerland); LDZ-4 Centrifuge (Beijing Harada Centrifuge Factory)

[0054] 2 Determination method

[0055] 2.1 Preparation of standard solution

[0056] Pipette 1ml of standard products of NAB, NAT, NNN, and NNK with a concentration of 0.5mg / ml respectively, place them in a 100mL volumetri...

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Abstract

The invention provides a method for measuring tobacco-specific N-nitrosamine in tobacco. The method adopts gas chromatography triple quadrupole mass spectrometry (GC-QQQ-MS / MS) to measure the content of tobacco-specific N-nitrosamine in tobacco and specifically comprises the following steps: 1, preparation of a standard solution; 2, sample pretreatment; 3, qualitative detection of samples; 4, quantitative detection of samples. According to the method for measuring tobacco-specific N-nitrosamine in tobacco, an optimally-selected mixed solvent is used for extraction, so that the sample pretreatment method is optimized; besides, simple and fast measurement is performed on the basis of high chromatographic fractionation capability and good selectivity and sensitivity of the GC-QQQ-MS / MS, so that higher sensitivity, accuracy and precision can be achieved, and the detection efficiency of four kinds of tobacco-specific N-nitrosamine in tobacco can be improved effectively.

Description

technical field [0001] The invention belongs to the technical field of detection of harmful substance components in tobacco, and in particular relates to a method for measuring tobacco-specific N-nitrosamines in tobacco. Background technique [0002] Tobacco-specific N-nitrosamines (TSNAs, Tobacco-Specific Nitrosamines) are non-volatile nitrosamines found only in tobacco and smoke. It is generally believed that tobacco-specific N-nitrosamines are formed through nitrification of tobacco alkaloids during tobacco curing. Among them, tobacco mainly contains four kinds, namely, N-nitroso demethylnicotine (NNN), 4-(methylnitrosoamino)-l-(3-pyridyl)-1-butanone (NNK ), N-nitrosoanabatin (NAB) and N-nitrosoanatabine (NAT). The weight analysis of previous animal or human toxicology studies shows that TSNA is one of the most carcinogenic substances in tobacco, and they have always been a hot topic in tobacco industry research. [0003] At present, the actual tobacco samples usually ...

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Application Information

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IPC IPC(8): G01N30/88
Inventor 刘鸿顾文博谢雯燕陆怡峰王磊君
Owner SHANGHAI TOBACCO GRP CO LTD
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