Extraction and determination method of special N-nitrosamine of tobacco in tobacco or tobacco product based on hydrophobic ionic exchange solid phase extraction
A technology of tobacco products and ion exchange, applied in the field of tobacco chemical inspection, can solve problems such as affecting the enrichment and purification effect of fillers, swelling and other problems, achieve good purification effect, improve accuracy and precision, and remove impurities and interfering substances. Effect
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Embodiment 1
[0074] In this embodiment, the method for extracting tobacco-specific N-nitrosamines based on hydrophobic ion exchange solid-phase extraction in tobacco or tobacco products comprises the following steps:
[0075] 1) Weigh 1.0 g of tobacco (accurate to 0.1 mg), put it into a 30 mL centrifuge tube with a stopper, accurately add 30 mL of ammonium acetate aqueous solution with a concentration of 10 mmol / L, and place it in an ultrasonic generator (purchased from Kunshan Ultrasound Instrument Co., Ltd., Model: KQ-700DB) for ultrasonic treatment, ultrasonic extraction for 30min, ultrasonic power 700W, frequency 40kHz, after standing for 5min, placed on a centrifuge for 5min, centrifugal speed 10000rpm, take the supernatant to obtain the crude extract; The pH value of the extract was adjusted to 4 before loading as the loading solution;
[0076] 2) Accurately weigh 500mg of HiCapt MCX solid-phase extraction filler into an empty solid-phase extraction column tube with a sieve plate, be...
Embodiment 2
[0100] According to the assay method described in Example 1, select other hundreds of tobacco or tobacco products, and measure the content of tobacco-specific N-nitrosamines, the results are as follows: figure 2 shown.
[0101] In other embodiments of the present invention, the pH of the crude extract can be adjusted to 3.4-11.0, further the pH of the crude extract in other embodiments of the present invention can be selected as 3.4, 6, 10.5 or 11, and the others are the same as in the implementation example 1. Since the filler in the HiCaptMCX solid-phase extraction column extracts the target analyte mainly by hydrophobic and cation exchange, most of the target analyte molecules exist in the protonated form when the pH is 4.0-6.0, and the cation exchange group in the filler In the form of negative ions, the hydrophobic interaction and cation exchange force between the filler and the target analyte are strong at this time, so it has the best extraction effect. Such as ima...
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