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A method for the determination of tobacco-specific n-nitrosamines in electronic cigarette liquid

A technology of electronic cigarette smoke liquid and determination method, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of inability to achieve accurate detection, detection sensitivity and removal of matrix interference, etc., so as to improve anti-interference ability and reduce detection. limit and improve sensitivity

Active Publication Date: 2016-09-07
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above determination methods are simple, they need to be improved in terms of detection sensitivity and removal of matrix interference. Therefore, accurate detection cannot be achieved for some tobacco-specific samples with low N-nitrosamine content.

Method used

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  • A method for the determination of tobacco-specific n-nitrosamines in electronic cigarette liquid
  • A method for the determination of tobacco-specific n-nitrosamines in electronic cigarette liquid
  • A method for the determination of tobacco-specific n-nitrosamines in electronic cigarette liquid

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] A method for the determination of tobacco-specific N-nitrosamines in electronic cigarette liquid:

[0024]1. Preparation of standard working solution: firstly, ethyl acetate / acetone (1 / 1, v / v) was used as solvent, and four tobacco-specific N-nitrosamine isotopes (NNN-d4, NAT-d4, NAB- d4 and NNK-d4) The standard substance is a solute, and a mixed internal standard solution with a concentration of 1 μg / mL is prepared; then ethyl acetate / acetone (1 / 1, v / v) is used as a solvent, and four kinds of tobacco-specific N -Nitrosamine (NNN, NAT, NAB, and NNK) standard products are solutes, and a series of mixed standard solutions with different concentrations containing internal standards are prepared, wherein the concentration gradients of NNN, NAT, and NAB in the mixed standard working solution are all 0.200, 0.500, 1.00, 2.00, 5.00, 10.0, 20.0, 50.0 ng / mL; the concentration gradient of NNK in the mixed standard working solution is 0.218, 0.545, 1.09, 2.18, 5.45, 10.9, 21.8, 54....

example 2

[0029] According to the determination method described in Example 1, another 21 commercially available e-cigarette liquids were selected, and the contents of tobacco-specific N-nitrosamines were determined. The results are shown in Table 5.

[0030]

[0031]

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PUM

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Abstract

The invention discloses a method for determining special tobacco N-nitrosamine in the cigarette liquid of an electronic cigarette. The method is characterized by comprising the following steps: diluting a cigarette liquid sample of the electronic cigarette by using a phosphate buffer solution containing ascorbic acid, and then performing extraction by using dichloromethane; concentrating an extracting solution, then performing determining by using online gel permeation chromatography-gas chromatography-tandem mass spectrometer, and performing quantification by using an internal standard method. The method for determining the special tobacco N-nitrosamine in the cigarette liquid of the electronic cigarette is brand new, and has the advantages of high sensitivity, good matrix removing effect and strong anti-jamming capability.

Description

technical field [0001] The invention belongs to the technical field of physical and chemical testing of electronic cigarettes, and specifically relates to a method for measuring the content of tobacco-specific N-nitrosamines in electronic cigarette liquid by using liquid-liquid extraction-gel permeation chromatography-gas chromatography-tandem mass spectrometry. Background technique [0002] N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitroso anatabine (NAB), and 4-(nitrosomethylamino)-1-beta -Pyridyl-1-butanone (NNK) is a ubiquitous carcinogen in cigarette smoke. Since these N-nitrosamines only exist in cigarette smoke or other tobacco products, they are called tobacco-specific N-nitrosamines. nitramines. As early as 1962, there were international articles reporting that tobacco contained N-nitrosamines and their potential carcinogenicity. Currently, tobacco-specific N-nitrosamines are listed as carcinogenic and probable carcinogens blacklisted by the Internat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 罗彦波陈小静姜兴益张洪非李雪刘洋朱风鹏李翔宇庞永强陈欢韩书磊侯宏卫
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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