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Extraction and determination method of tobacco special N-nitrosamine in electronic cigarette liquid based on hydrophobic ion exchange solid phase extraction

An electronic cigarette liquid and ion exchange technology, applied in the field of tobacco chemical inspection, can solve problems such as swelling, affecting filler enrichment and purification effect, etc., to achieve the effect of improving accuracy and precision, good purification effect, and high removal efficiency

Inactive Publication Date: 2017-08-18
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid-phase extraction filler in this scheme is N-vinylpyrrolidone-benzenesulfonic acid copolymer, and the organic polymer filler will swell in the organic solvent, which will affect the enrichment and purification effect of the filler to a certain extent
In addition, the program did not optimize various parameters that affect the extraction effect

Method used

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  • Extraction and determination method of tobacco special N-nitrosamine in electronic cigarette liquid based on hydrophobic ion exchange solid phase extraction
  • Extraction and determination method of tobacco special N-nitrosamine in electronic cigarette liquid based on hydrophobic ion exchange solid phase extraction
  • Extraction and determination method of tobacco special N-nitrosamine in electronic cigarette liquid based on hydrophobic ion exchange solid phase extraction

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Experimental program
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Effect test

Embodiment 1

[0057] In this embodiment, the extraction method of tobacco-specific N-nitrosamines in electronic cigarette liquid based on hydrophobic ion exchange solid-phase extraction includes the following steps:

[0058] 1) Weigh 1.0000g of e-cigarette liquid (accurate to 0.1mg), put it into a 15mL stoppered centrifuge tube, accurately add 10mL of ammonium acetate aqueous solution with a concentration of 10mmol / L, and place it in an ultrasonic generator (purchased from Kunshan Ultrasonic Instrument Co., Ltd. Company, model: KQ-700DB) on ultrasonic for 10min, ultrasonic power 700W, frequency 40kHz, stand still for 5min, get the diluent of e-cigarette liquid, adjust the pH value of the diluent of e-cigarette liquid to 4, and use it as the loading solution for later use; Weigh 500mg of HiCapt MCX solid-phase extraction filler into an empty solid-phase extraction column tube with a sieve plate, beat continuously to fill the filler evenly, put the sieve plate on the upper end cover and press ...

Embodiment 2

[0080] According to the determination method described in Example 1, another 8 kinds of e-cigarette liquids were selected, and the content of tobacco-specific N-nitrosamines was determined, and the results were as follows: figure 2 shown.

[0081] In other embodiments of the present invention, the amount of HiCapt MCX solid-phase extraction filler can also be 50 mg, 100 mg, 200 mg, 300 mg or 1000 mg, and the others are the same as in Embodiment 1. Depend on image 3 The optimization results of HiCapt MCX solid phase extraction filler quality shows that the quality of the filler is in the range of 50-1000mg. With the continuous improvement of the filler quality, the peak area of ​​the target analyte increases significantly. When the quality of the HiCapt MCX solid phase extraction filler reaches At 500 mg, continue to increase the mass of the filler, but the peak area of ​​the target analyte does not increase significantly. Considering the extraction effects of all target ana...

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Abstract

The invention relates to an extraction and determination method of tobacco special N-nitrosamine in electronic cigarette liquid based on hydrophobic ion exchange solid phase extraction, and belongs to the technical field of tobacco chemical inspection. According to the extraction method of tobacco special N-nitrosamine in electronic cigarette liquid, dilution liquid of the electronic cigarette liquid flows through an activated HiCapt MCX solid phase extraction column; a methanol water solution is used for performing leaching; then, any one kind of materials from acetone, acetone and ammonia water mixed solution and acetone and formic acid mixed solution is used for elution; a finished product is obtained. The extraction method of tobacco special N-nitrosamine in electronic cigarette liquid has the advantages that impurities and interferents can be effectively removed; the accuracy and the precision of the determination method can be improved. The relative recovery rate of the method is between 92.2 and 108.9 percent; the within-day precision is lower than 4.6 percent; the intra-day precision is lower than 4.2 percent.

Description

technical field [0001] The invention relates to a method for extracting and measuring tobacco-specific N-nitrosamines in electronic cigarette liquid based on hydrophobic ion-exchange solid-phase extraction, and belongs to the technical field of tobacco chemical inspection. Background technique [0002] N-nitrosamine is a general term for a class of compounds containing -N-NO in structure, which can be divided into volatile N-nitrosamine and non-volatile N-nitrosamine according to the difference in physical properties. Among them, volatile N-nitrosamines are a class of carcinogens that have received widespread attention. They are not only important substances in the Hoffman list and the US Food and Drug Administration's "List of Harmful and Potentially Harmful Substances in Tobacco Products and Smoke" , is also an important ingredient in the list of "28 harmful substances in smokeless tobacco products" of the International Agency for Research on Cancer. Therefore, the determ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 罗彦波庞永强姜兴益朱风鹏李翔宇张洪非胡少东闫瑞波陈小静
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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