Method for simultaneously testing specific N-nitrosamine and polycyclic aromatic hydrocarbon of tobacco in main stream smoke of cigarette on basis of tip-microextraction

A polycyclic aromatic hydrocarbon and determination method technology, applied in the field of cigarette smoke chemical inspection, can solve the problems affecting the maintenance frequency and maintenance cost of the instrument

Active Publication Date: 2016-04-27
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Abstract
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  • Application Information

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Problems solved by technology

This method directly filters the mainstream smoke extract and then conducts line gel chromatography-gas chromatography-tandem mass spect

Method used

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  • Method for simultaneously testing specific N-nitrosamine and polycyclic aromatic hydrocarbon of tobacco in main stream smoke of cigarette on basis of tip-microextraction
  • Method for simultaneously testing specific N-nitrosamine and polycyclic aromatic hydrocarbon of tobacco in main stream smoke of cigarette on basis of tip-microextraction
  • Method for simultaneously testing specific N-nitrosamine and polycyclic aromatic hydrocarbon of tobacco in main stream smoke of cigarette on basis of tip-microextraction

Examples

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Embodiment 1

[0023] A method for simultaneous determination of tobacco-specific N-nitrosamines and polycyclic aromatic hydrocarbons in cigarette mainstream smoke after purification by tip-micro-solid-phase extraction. The specific steps of the operation process are:

[0024] The first step is the preparation of standard series solutions: using ethyl acetate and acetone with a volume ratio of 1:1 as solvents, four tobacco-specific N-nitrosamines (NAB, NAT, NNN and NNK) and three polycyclic aromatic hydrocarbons (BaP, BaA, and Chry) standard products are solutes, NAB-d4, NAT-d4, NNN-d4, NNK-d4, and BaP-d12 are internal standards, and standard series solutions containing internal standards with different concentrations are prepared;

[0025] The second step, the preparation of the sample solution: smoke the balanced and screened cigarettes with a linear smoking machine according to the standard conditions specified in GB / T19609-2004, ISO4387:2000, and smoke 5 cigarettes for each experiment. P...

Embodiment 2

[0042] According to the method described in Example 1, the cigarettes were sucked and the tip-micro solid phase extraction was purified, and the standard series solution and the sample solution were analyzed by gas chromatography-tandem mass spectrometer. The release of tobacco-specific N-nitrosamines and polycyclic aromatic hydrocarbons in mainstream smoke of different brands of cigarettes were determined by the above method and the standard method. The results are shown in Table 6: there is no significant difference between the detection results of the method of the present invention and the standard method.

[0043] Table 6. The detection results of tobacco-specific N-nitrosamines and polycyclic aromatic hydrocarbons in different brands of cigarettes (ng / cigarette, the results of standard methods are in brackets)

[0044] Numbering

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Abstract

The invention belongs to the technical field of chemical examination of cigarette smoke and particularly discloses a method for simultaneously testing specific N-nitrosamine and polycyclic aromatic hydrocarbon of tobacco in main stream smoke of a cigarette on the basis of tip-microextraction. According to the method, a smoking machine is used for smoking the cigarette, a Cambridge filter is used for capturing the main stream smoke of the cigarette, a solution containing internal labels is used for extracting the filter, and an extracting solution is detected after being subjected to tip-micro solid-phase extraction purification. Compared with a standard method, the method is used for testing two compounds simultaneously, and the testing efficiency is improved; detection can be performed after tip-micro solid-phase extraction purification of the extracting solution of the smoke, and the method has the advantages of simplicity and convenience in operation, fastness and efficiency.

Description

technical field [0001] The invention belongs to the technical field of chemical inspection of cigarette smoke, and in particular relates to a method for simultaneously measuring tobacco-specific N-nitrosamines and polycyclic aromatic hydrocarbons in cigarette mainstream smoke based on tip-microextraction. Background technique [0002] Polycyclic aromatic hydrocarbons are the products of incomplete combustion of tobacco under high temperature and anoxic conditions. At present, about 30 carcinogenic condensed aromatic hydrocarbons have been identified in mainstream cigarette smoke. In 1967, Hoffmann et al. proved that polycyclic aromatic hydrocarbons Carcinogenicity of aromatic hydrocarbons, and subsequently published medical reports identified benzo[a]pyrene as the most important carcinogen among polycyclic aromatic hydrocarbons. Tobacco-specific N-nitrosamines mainly refer to N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabatin (NAB) and 4-( Nitrosomethyl...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 罗彦波陈小静张洪非李雪刘洋李翔宇姜兴益朱风鹏庞永强
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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