39 results about "Autologous Fat Graft" patented technology

A method is disclosed for preparing a soft tissue site, and augmenting the soft tissue site, such as the breast(s) of a subject through use of devices that exert a distractive force on the breast(s) and grafting of autologous fat tissue such as domes with sealing rims for surrounding each of the breasts and a regulated pump. The method for preparing the soft tissue site, and enhancing fat graft results, entails application of the distracting force to the targeted soft tissue site for several weeks prior to the graft procedure. A related aspect of the invention includes following the preparation steps by transfer of fat from other areas of the subject to the subject's breast(s), and then reapplication of the distractive force to the breast(s) that received the autologous fat graft. Alternatively, fat from genetically related sources may be used, and the fat may be further processed prior to injection. Substantial breast augmentation, high rates of graft survival and negligible graft necrosis (data demonstrating 80% survival and only 20% necrosis is presented) or calcification result from the practice of these methods.

The invention details a cryoprotectant and the methods to prepare biological tissue and related business methods and systems. The invention is directed to methods for collecting, washing, cyropreserving, recovering, and return of lipoaspirates to physicians for autologous adipose tissue transfer procedures in patients. In an embodiment, the invention is directed to a cryoprotectant solution for cryopreserving biological tissue essentially of a polyol and a crysalloid.

The invention relates to an application of autologous adipose-derived mesenchymal stem cells in preparation of medicines for treating joint diseases. The application is characterized in that adipose-derived mesenchymal stem cells (Ad-MSCs) separated and extracted from autologous adipose tissues of the patient are injected to the diseased articular cavity in a large dose once. The adopted mesenchymal stem cells have immunophenotyping and representing biological marks of Ad-MSC through flow cytometry authentication, and can be directionally induced and differentiated to osteoblast and chondrocyte. Primary cells are directly injected to the articular cavity of the diseased joint to treat various joint diseases. With the adoption of the autologous adipose-derived mesenchymal stem cells as the primary cells, the cells have better differentiative potential and the ability of inhibiting inflammation and immunoregulation ability. The adipose-derived mesenchymal stem cell transplant provides a novel effective means for treating osteoarticular diseases. The method is simple and feasible, and the quantity of cells collected is great and the trauma is small. The autologous mesenchymal stem cells of the patient are applied without invading related ethics, so that the risk of immunological rejection, infectious diseases and the like is avoided.

The invention relates to a stem cell repairing material as well as a preparation method and application thereof. The stem cell repairing material comprises a stem cell and a cell carrier, wherein the stem cell is an autologous adipose-derived mesenchymal stem cell (ADSC) with a concentration of 105-108 / ml, and the cell carrier is autoserum, normal saline (0.9%) or a glucose injection (5%). In the invention, adopted seed cells are prepared through separating and purifying autologous adipose tissues of patients, thereby avoiding the ethical disputes and the immunological rejection. The material drawing of the adipose tissues can be performed by using an instrument suction method, which is simple in operation and can bring less traumas and pains to the patients. The cell carrier used in the invention is the autoserum, normal saline (0.9%) or glucose injection (5%), therefore, the cell carrier mixed with mesenchymal stem cells and other ingredients can be injected into the patents by virtue of syringes. By using the stem cell repairing material provided by the invention, the operation process is simple, no scar is left, and the wounds and pains for the patients are avoided, therefore, the stem cell repairing material can be easily accepted by the patients.

The invention discloses a preparation method and an application of a biological scaffold material and SVF assistant adipose tissue. Under an aseptic condition, the biological scaffold material, a stromal vascular fraction (SVF) prepared by digesting purified adipose granules with a medical-graded collagenase, and the purified adipose particles are evenly mixed according to a certain proportion to prepare an adipose graft for filling. The SVF is a mixture of adipose mesenchymal stem cells and various cells, is transplanted after being mixed with autogenous adipose particles, and can be continued to be developed into a mature adipose tissue in vivo; the biological scaffold material can provide a support for transplanted adipose, allows the transplanted tissue to be prone to revascularization, and improves the survival rate of the transplanted tissue; the transplanted tissue is suitable for being injected with a 16 G or 18 G needle, facilitates clinical application, and can be used for repairing of concave deformity (such as tempora and cheek concave), beautifying packing of faces and chests, soft tissue function reconstruction and the like; and especially in the beautifying packing, the prepared transplanted adipose tissue can promote shape recovery and prevent liquefactive necrosis.

The invention relates to a human autologous adipose mesenchymal stem cell serum-free culture method which particularly includes the steps: selecting sterile adipose tissues and performing first digestion to obtain first cell suspension; performing second digestion on undigested adipose tissues to obtain second cell suspension; combining the first cell suspension and the second cell suspension andthen performing culture, passage and identification to obtain human autologous adipose mesenchymal stem cells. According to the adipose mesenchymal stem cell culture method, primary cell separation, multiplication culture and cryopreservation systems are serum-free culture media, and the risk of animal derived ingredient addition is completely avoided. The method is a two-step enzymic digestion method, by the aid of the specific proportion of digestive enzyme and the adipose tissues, the adipose tissues sufficiently contact with enzyme solution and can be sufficiently digested, the cells cannot be damaged, primary cells are high in yield, good in activity, high in repeatability and good in safety, and the method meets clinical application standards.

Methods and kits for producing cellular fractions enriched in adipose derived stem cells. Methods are provided where adipose tissue obtained from liposuction is enzymatically treated using a solution containing collagenase and divalent cations prior to the application of traditional methods of stromal-vascular fraction isolation. The enzymatic solutions may contain collagenase types I and II to a final concentration of about 0.001 mg / ml to 0.010 mg / ml. The divalent cations may be present as calcium, magnesium, and zinc chloride. The final concentration of calcium, magnesium, and zinc may range from about 0.001 to 0.1 micromolar; about 0.005 to 0.5 micromolar; and about 0.0015 to 0.15 micromolar, respectively. The enzymatic solutions may be generated using a kit where the collagenase and divalent components are held in separate containers until just prior to use. The cellular fractions isolated in this manner may be used in autologous fat grafts in therapeutic applications.

The invention discloses a tissue engineering biological material for transplanting and restoring a peripheral nerve defect. The tissue engineering biological material is prepared through the following steps: firstly, preparing an acellular nerve allograft scaffold by using a chemical extraction method; then culturing an adipose derived stem cell by using fatty tissues collected by a target receptor in an early debridement; finally carrying out induction in vitro by using an exogenous schwann cell endogenus neurotrophic factor to construct a tissue engineered peripheral nerve for later use. The acellular nerve allograft scaffold subject to chemical extraction has no immunogenicity and has a network structure most similar to an organism nerve and excellent biocompatibility, and meanwhile, a stem cell is controllably induced in vitro by using the schwann cell endogenus neurotrophic factor and is differentiated into schwann-liked cells in combination with an ideal scaffold and then transplanted in vivo to become effectiveness.

The invention belongs to the technical field of regenerative medicine and discloses a method for preparing an adipose-derived ECM (extracellular matrix) through built-in ultrasonic waves, which comprises steps of centrifugation, ultrasonic crushing, centrifugal collection and the like. According to the preparation method, most mature adipose cells in adipose tissue are crushed through ultrasonic waves, manure adipose cells and SVF cells are removed, the ECM is prepared, and too low ECM content and cell factor loss caused by complex operation are avoided effectively. According to the method, the ECM rich in cell factors is prepared without addition of exogenous chemical substances, the prepared ECM contains no exogenous substances, and adipose autotransplantation and effectiveness of the ECM as a biological filling material can be improved. The adipose-derived ECM material prepared with the method can be applied to autotransplantation stem cell therapy, reduces complications caused by adipose transplantation, increases the survival rate of transplanted adipose and has broad application prospects.

The invention discloses an autologous adipose stem cell, and specifically relates to an autologous adipose stem cell experimental method of PRP for treating osteoarthritis. The method includes the following steps: selecting the components of autologous stem cells as the patient's autologous adipose stem cells and high-concentration platelet plasma, isolating and culturing the adipose stem cells for 2 to 4 weeks, obtaining cultured adipose stem cells, adding the high-concentration platelet plasma into the adipose stem cells to obtain a mixture of adipose stem cells, adding growth factors and conversion factors, uniformly mixing, filtering, removing filter residue, taking a clear filtrate as an injection, and selecting injection points at joints where the patient suffers from osteoarthritisfor injection. The adipose stem cell mixture can effectively reduce the inflammatory response of osteoarthritis and the patient's own immune response, and has a significant effect on the treatment ofosteoarthritis.

The invention provides a culture method for a stent-free three-dimensional gel culture system of mesenchymal stem cells. The culture method comprises the following steps that the adipose mesenchymal stem cells are inoculated into a culture dish, inducing culture is carried out in an inducing medium containing ascorbic acid-2-phosphate (ASC2P) gel, collagen is secreted from the adipose mesenchymalstem cells under the action of the inducing medium to complete intercellular bonding, and the stent-free three-dimensional gel of the mesenchymal stem cells with a hydrogel state with a three-dimensional structure is formed. According to the culture method, autologous fat is used as a source of stem cells, the stent-free three-dimensional gel of the mesenchymal stem cells of an external organizational framework is not added, the hydrogel state is showed, cartilage damages of different shapes can be adopted, a rejection reaction is avoided, and cartilage is advantageously regenerated and repaired.

The invention provides a hair growth injection solution based on an autologous fat stem cell exosome, and a preparation method. The hair growth injection solution consists of the autologous fat stem cell exosome and water used for injection. The preparation method of the hair growth injection solution comprises the following steps of: S1: selecting autologous fat; S2: preparing fat stem cells; S3: preparing the fat stem cell exosome; and S4: preparing the hair growth injection solution of the fat stem cell exosome. The hair growth injection solution based on the autologous fat stem cell exosome can effectively utilize autologous tissues and has the advantages that the hair growth injection solution is easy in operation, does not have an immunogenicity reaction risk, is high in activity, has an obvious effect, has a low preparation cost, is high in operability and the like.

The invention provides a preparation method of an exosome, the exosome prepared by the preparation method and application of the exosome. The preparation method comprises the following steps: (1) digesting autologous adipose tissues with trypsin digestive juice, and carrying out cleaning to obtain adipose cells; (2) culturing the adipose cells obtained in the step (1) in a culture medium to obtain a cell suspension; and (3) mixing the cell suspension obtained in the step (2) with tranexamic acid, then, carrying out culturing, and carrying out collecting to obtain the tranexamic-acid-modified exosome generated by autologous mesenchymal adipose-derived stem cells. Effective components of the exosome preparation comprise the exosome generated by the autologous mesenchymal adipose-derived stem cells and modification of the tranexamic acid, and the exosome preparation for repairing burn wounds needs to be capable of promoting wound healing, reducing melanin deposition of the wounds and reducing traces after wound healing.

The invention provides an extraction reagent and extraction method of human autologous stromal vascular fractions SVF. The extraction method comprises the steps of collecting adipose tissue, performing cleaning and centrifuging, adding isopyknic SVF extraction reagents, performing vibration and digestion, performing centrifuging to obtain precipitates, and performing cleaning, centrifuging and filtering on the precipitates so as to obtain SVF cells. A trehalose solution has protection effects on cell membrane proteins, a collagenase solution and a gelatine solution can promote cell proliferation and migration, the consistency of a solution can be improved, the shearing force of the solution can be reduced, and the objectives of protecting the cells and increasing the survival rate of the cells in the adipose tissue can be achieved.

The invention relates to a nerve / blood vessel network based on animal adipose tissue-derived hydrogel and a construction method and application thereof, the nerve / blood vessel network comprises a three-dimensional neural network and an artificial blood vessel, the three-dimensional neural network uses hydrogel prepared from an acellular matrix of animal adipose tissue as a substrate to culture nerve cells, the neural network with at least two layers of cell structures is constructed in a stacking manner, and the preparation material of the hydrogel can be taken from autologous adipose tissue, so that the neural / vascular network based on the animal adipose tissue source hydrogel constructed by the invention can be transplanted to the autologous body and is free from immunological rejection. The neural / vascular network based on the animal adipose tissue-derived hydrogel can accurately simulate the microenvironment of neural network growth, can monitor the electrical signal change of neuron activity in real time, and is of great significance in promoting brain science research.

The invention discloses application of mesenchymal stem cells combined with PRP (platelet-rich plasma) in preparation of a medicine for treating vulva atrophy. According to the invention, the autologous adipose-derived mesenchymal stem cells and the PRP are combined for treating the vulva atrophy, the autologous adipose-derived mesenchymal stem cells can be differentiated into adipose cells subcutaneously, the PRP can promote the improvement of capillary vessels, and the autologous adipose-derived mesenchymal stem cells and the PRP interact with each other, so that the atrophic vulva cells can be repaired and regenerated; the traditional Chinese medicine composition can effectively treat and improve symptoms of a patient remarkably. compared with an existing operation of repairing vulva atrophy, the traditional Chinese medicine composition is simpler in treatment and more simplified in operation steps, autologous cells and PRP are adopted, the rejection risk is eliminated, and the safety is higher.

The invention relates to the technical field of medical cosmetology, in particular to a full-automatic autologous fat breast enlargement system and technology.The full-automatic autologous fat breast enlargement system comprises a shell, moving mechanisms are arranged on the front side and the rear side of the shell, first motors are fixedly connected to the outer sides of the moving mechanisms, and output shafts of the first motors penetrate through the moving mechanisms and are fixedly connected with driving wheels; the bottom of the inner cavity of the shell is fixedly connected with a gear plate; by arranging the shell, the moving mechanism, the first motor, the driving wheel, a gear plate, a hydraulic rod, a gear disc and an adjusting mechanism, the device has the advantages of being capable of automatically moving, flexibly adjusting the injection angle and preventing an injection needle from deviating, the injection efficiency can be improved, fat particles are washed and sterilized, the needle angle can be kept unchanged, and the injection efficiency is improved. And therefore, the working intensity of the medical beauty staff is effectively reduced, and the problems that the needle angle is unstable, the injection position deviates and the body tissue is easily injured due to manual operation at present are solved.

The invention relates to the field of stem cells, and provides a method for repairing diseased pancreatic islets by specific stem cells, which is used to improve the repairing effect of pancreatic islets. A method for repairing diseased islets with specific stem cells provided by the present invention, comprising: S11. The cells obtained after centrifuging autologous adipose-derived mesenchymal cells are cultured with adipose-derived stem cells and passed to the P3 generation; S12. The P3-generation adipose-derived mesenchymal Stem cells were digested and resuspended in adipose-derived mesenchymal stem cell culture medium, cultured for 36 hours, then half of the culture medium was replaced every 72 hours, cultured for 8 days, and cell fluid was obtained, and the medium contained pancreatic islet decellularized matrix; S13. In PBS The nanoporous micro-stent is added into the solution, dispersed and mixed with the cell solution to obtain the islet repair solution, and the islet repair solution is injected into the islet. The specific stem cells selected from the autologous body can improve the success rate of islet repair and promote the timely transformation of diseased islets into normal tissues.

Methods and kits for producing cellular fractions enriched in adipose derived stem cells. Methods are provided where adipose tissue obtained from liposuction is enzymatically treated using a solution containing collagenase and divalent cations prior to the application of traditional methods of stromal-vascular fraction isolation. The enzymatic solutions may contain collagenase types I and II to a final concentration of about 0.001 mg / ml to 0.010 mg / ml. The divalent cations may be present as calcium, magnesium, and zinc chloride. The final concentration of calcium, magnesium, and zinc may range from about 0.001 to 0.1 micromolar; about 0.005 to 0.5 micromolar; and about 0.0015 to 0.15 micromolar, respectively. The enzymatic solutions may be generated using a kit where the collagenase and divalent components are held in separate containers until just prior to use. The cellular fractions isolated in this manner may be used in autologous fat grafts in therapeutic applications.

The present invention relates to the technical field of reconstructive plastic surgery and cosmetology, and particularly to a biomimetic chitosan filler for preventing capsular contracture and preparation method thereof. The biomimetic chitosan filler for preventing capsular contracture includes chitosan gel, cross-linked sodium hyaluronate, surfactant, isoosmotic regulator, autologous adipose-derived stem cells, pH adjusting agent and water. The amount of the pH adjusting agent is to adjust a pH value of the chitosan filler to be within a range of 6.5-7.5. The chitosan filler prepared according to the present invention can reduce the inflammation caused by post-operative silica gel exudation, and prevent the occurrence and development of capsular contracture.

The invention discloses a clinically ready-to-use vital preservation method for adipose-derived stem cells. The method comprises the following steps of suspending the adipose-derived stem cells into a non-immunogenicity human-derived serum or a human platelet rich plasma with the volume fraction being 10 + / -0.5 percent, and standing and preserving at 4 DEG C. The clinically ready-to-use vital preservation method for the adipose-derived stem cells provided by the invention is applicable to a ready-to-use ADSCs clinic treatment applying technique, and the ADSCs are provided with the optimal preservation temperature and the most suitable cell preservation medium during transportation or a short-time preservation process, so that the clinically ready-to-use ADSCs maintains the optimal cell activity before being applied in a human body, the effectiveness and the safety of the application of the clinical adipose-derived stem cells are improved, and clinical application and a clinical technology of the autologous adipose-derived stem cells are conveniently popularized. The preservation method provided by the invention has the advantages of conformation to theclinical standard, economy, simplicity, convenience, and capability of better maintaining cell activity. With popularization and application of the clinically ready-to-use vital preservation method for the adipose-derived stem cells, the effectiveness and the safety of the clinical application of cell products can be improved, and great economic and social benefits can be achieved.

The invention discloses a method for improving infertility by employing stem cells. Firstly, autologous adipose-derived stem cells of a patient are extracted in a hospital; then, the autologous adipose-derived stem cells are transported under cold storage to a cell preparation center by cold-chain transportation; then, the autologous adipose-derived stem cells are cultured in a culture flask for passaging and proliferation in a good manufacturing practice (GMP) lab; after the autologous adipose-derived stem cells are cultured for 29 days to 35 days, the proliferated autologous adipose-derivedstem cells are transported back to the hospital in a manner of cold-chain transportation; the proliferated autologous adipose-derived stem cells are injected back into the body of the infertility patient; and the number of the autologous adipose-derived stem cells injected each time is 20000-60000 units per kg, the autologous adipose-derived stem cells are injected once at intervals of 8-10 days,every four times of injection is taken as a course of treatment, and the autologous adipose-derived stem cells need to be continuously injected for 10-20 courses of treatment in total. The method disclosed by the invention can bring new hope for infertility patients; the stem cells can repair the endometrium of an infertility woman to make the endometrium regenerated, and premature ovarian failureis effectively improved, thus being conducive to androlepsis.

A triple-chambered container includes: a main container body with a first chamber which has an opening at its distal end; a main barrel formed and movable longitudinally within the first chamber, the main barrel defining therein a second chamber for receiving fluids, the main barrel further having an apertured stopper at its distal end; a second barrel formed within the main barrel, the second barrel defining a third chamber for receiving fluids, the second barrel being movable longitudinally within and with respect to the main barrel, the second barrel having a distal end which is engageable and disengageable with the apertured stopper; a shaft adapted to fit within the second barrel, the shaft being movable longitudinally within and with respect to the second barrel, the shaft having a distal end which is engageable and disengageable with an aperture in the second barrel; a device for controlling engaging and disengaging of the distal end of the shaft with the aperture of the second barrel. The first chamber, the second chamber and the third chamber may be selectively moved to receive and discharge fluids with respect to one another.

A method of curing (or at least treating) HIV, by obtaining small tissue mesenchymal stem cells (stMSC) from adipose tissue of a patient with HIV or from a syngeneic donor or an immuno-compatible donor, wherein said stMSC are i) 4-6 μm in diameter, ii) pluripotent, and iii) have CD11b−, CD34−, CD45−, CD 29, CD 49, Oct 4 and SSEA 4 surface markers when harvested. Preferred sources are autologous adipose tissue. Next those stMSCs are gene edited to provide CCR5− stMSCs and optionally amplified by growth in culture and / or by cell selection for gene edited stMSCs and then re-introducing into the patient. Preferably, the CCR5− stMSCs will re-populate the patient with stem cells that can no longer act as latent HIV reservoirs and can differentiate into immune cells that are HIV resistant. If desired, the patient can be first treated, e.g., by radiation, to destroy the bone marrow cells before the re-introduction. However, this may not be necessary for patient who already has significant destruction of immunity by HIV.

The invention discloses a boosting device with dominant pressure adjustment and an injection and sucking system. The boosting device with dominant pressure adjustment is used for assisting an injectorto inject and suck autologous lipid on the basis of dominant pressure adjustment; the injector comprises a work drum, a needle head which is mounted on one side of the work drum, and a core rod whichis moveably embedded into the work drum; skit sides extending in a circumferential direction are arranged on sides, far away from the needle head, of the core rod and the work drum; and the boostingdevice with dominant pressure adjustment comprises a display setting unit and a booster in wireless communication connection with the display setting unit. The boosting device is capable of intelligently sucking lipid of a human body on the basis of pressure adjustment, and injury to the human body can be reduced in the liposuction process; and the lipid can be injected intelligently on the basisof pressure adjustment to implement a cosmetic surgery, the number and part of injected lipid can be adjusted according to pressure variation in the injection process, survival of the lipid in a bodycan be promoted, and the healing speed of wounds can be increased.

The invention discloses a breast augmentation prosthesis based on a five-line inner edge and an implantation method of the breast augmentation prosthesis. The breast augmentation prosthesis comprisesa prosthesis body, a convex shell is arranged on the outer side of the prosthesis body, a concave area is arranged on the inner side of the convex shell, implanter fixing membranes are arranged at thetwo ends of the inner side of the concave area, and a filling area is arranged in the middle of the inner side of the concave area. The implantation method comprises the steps of anesthesia, incision, separation, implantation and suturing. According to personal breast form conditions and requirements of an aesthetic person, an ideal breast form meeting modern aesthetic appreciation is created according to personal characteristics, two methods of prosthesis breast augmentation and autologous fat breast augmentation are combined, breast form and hand feeling creation is focused, dynamic, touchand texture indexes of breasts are effectively improved after an operation, the formed breasts naturally fluctuate along with the body postures during sitting, lying, jumping and standing, the radianis natural, the dynamic rhythm beauty is manifested, the operation is safer, the whole process is clear, blood vessels, nerves and surrounding tissues are not damaged, and the breastfeeding cannot beinfluenced.