37 results about "Autologous Fat Graft" patented technology

A method is disclosed for preparing a soft tissue site, and augmenting the soft tissue site, such as the breast(s), scar, depression, or other defect, of a subject through use of devices that exert a distractive force on the breast(s) and grafting of autologous fat tissue such as domes with sealing rims for surrounding each of the soft tissue site and a regulated pump. The method for preparing the soft tissue site, and enhancing fat graft results, entails application of the distracting force to the targeted soft tissue site at least intermittently for some period of time and preferably several weeks prior to the graft procedure. A related aspect of the invention includes following the preparation steps by transfer of fat from other areas of the subject to the subject's soft tissue site, and then reapplication of the distractive force to the soft tissue site that received the autologous fat graft. Alternatively, fat from genetically related sources may be used, and the fat may be further processed prior to injection. Substantial soft tissue augmentation, high rates of graft survival and negligible graft necrosis (data demonstrating 80% survival and only 20% necrosis is presented) or calcification result from the practice of these methods.

The invention relates to an application of autologous adipose-derived mesenchymal stem cells in preparation of medicines for treating joint diseases. The application is characterized in that adipose-derived mesenchymal stem cells (Ad-MSCs) separated and extracted from autologous adipose tissues of the patient are injected to the diseased articular cavity in a large dose once. The adopted mesenchymal stem cells have immunophenotyping and representing biological marks of Ad-MSC through flow cytometry authentication, and can be directionally induced and differentiated to osteoblast and chondrocyte. Primary cells are directly injected to the articular cavity of the diseased joint to treat various joint diseases. With the adoption of the autologous adipose-derived mesenchymal stem cells as the primary cells, the cells have better differentiative potential and the ability of inhibiting inflammation and immunoregulation ability. The adipose-derived mesenchymal stem cell transplant provides a novel effective means for treating osteoarticular diseases. The method is simple and feasible, and the quantity of cells collected is great and the trauma is small. The autologous mesenchymal stem cells of the patient are applied without invading related ethics, so that the risk of immunological rejection, infectious diseases and the like is avoided.

Methods and kits for producing cellular fractions enriched in adipose derived stem cells. Methods are provided where adipose tissue obtained from liposuction is enzymatically treated using a solution containing collagenase and divalent cations prior to the application of traditional methods of stromal-vascular fraction isolation. The enzymatic solutions may contain collagenase types I and II to a final concentration of about 0.001 mg / ml to 0.010 mg / ml. The divalent cations may be present as calcium, magnesium, and zinc chloride. The final concentration of calcium, magnesium, and zinc may range from about 0.001 to 0.1 micromolar; about 0.005 to 0.5 micromolar; and about 0.0015 to 0.15 micromolar, respectively. The enzymatic solutions may be generated using a kit where the collagenase and divalent components are held in separate containers until just prior to use. The cellular fractions isolated in this manner may be used in autologous fat grafts in therapeutic applications.

The invention relates to the field of stem cells, and provides a method for repairing diseased pancreatic islets by specific stem cells, which is used to improve the repairing effect of pancreatic islets. A method for repairing diseased islets with specific stem cells provided by the present invention, comprising: S11. The cells obtained after centrifuging autologous adipose-derived mesenchymal cells are cultured with adipose-derived stem cells and passed to the P3 generation; S12. The P3-generation adipose-derived mesenchymal Stem cells were digested and resuspended in adipose-derived mesenchymal stem cell culture medium, cultured for 36 hours, then half of the culture medium was replaced every 72 hours, cultured for 8 days, and cell fluid was obtained, and the medium contained pancreatic islet decellularized matrix; S13. In PBS The nanoporous micro-stent is added into the solution, dispersed and mixed with the cell solution to obtain the islet repair solution, and the islet repair solution is injected into the islet. The specific stem cells selected from the autologous body can improve the success rate of islet repair and promote the timely transformation of diseased islets into normal tissues.

The present invention relates to a method for using a freeze-dried hyaline cartilage powder to produce a composition for regenerating cartilage, and a composition for regenerating cartilage produced by using the method, the method comprising: A) a step for preparing hyaline cartilage; B) a step for freeze-drying and crushing the hyaline cartilage, and producing a freeze-dried hyaline cartilage powder; C) a step for producing an adipose tissue extract from autologous adipose tissue; and D) a step for producing a composition which is for regenerating cartilage and including the freeze-dried hyaline cartilage powder and the adipose tissue extract.

Methods and kits for producing cellular fractions enriched in adipose derived stem cells. Methods are provided where adipose tissue obtained from liposuction is enzymatically treated using a solution containing collagenase and divalent cations prior to the application of traditional methods of stromal-vascular fraction isolation. The enzymatic solutions may contain collagenase types I and II to a final concentration of about 0.001 mg / ml to 0.010 mg / ml. The divalent cations may be present as calcium, magnesium, and zinc chloride. The final concentration of calcium, magnesium, and zinc may range from about 0.001 to 0.1 micromolar; about 0.005 to 0.5 micromolar; and about 0.0015 to 0.15 micromolar, respectively. The enzymatic solutions may be generated using a kit where the collagenase and divalent components are held in separate containers until just prior to use. The cellular fractions isolated in this manner may be used in autologous fat grafts in therapeutic applications.

The present invention relates to the technical field of reconstructive plastic surgery and cosmetology, and particularly to a biomimetic chitosan filler for preventing capsular contracture and preparation method thereof. The biomimetic chitosan filler for preventing capsular contracture includes chitosan gel, cross-linked sodium hyaluronate, surfactant, isoosmotic regulator, autologous adipose-derived stem cells, pH adjusting agent and water. The amount of the pH adjusting agent is to adjust a pH value of the chitosan filler to be within a range of 6.5-7.5. The chitosan filler prepared according to the present invention can reduce the inflammation caused by post-operative silica gel exudation, and prevent the occurrence and development of capsular contracture.

The invention discloses a clinically ready-to-use vital preservation method for adipose-derived stem cells. The method comprises the following steps of suspending the adipose-derived stem cells into a non-immunogenicity human-derived serum or a human platelet rich plasma with the volume fraction being 10 + / -0.5 percent, and standing and preserving at 4 DEG C. The clinically ready-to-use vital preservation method for the adipose-derived stem cells provided by the invention is applicable to a ready-to-use ADSCs clinic treatment applying technique, and the ADSCs are provided with the optimal preservation temperature and the most suitable cell preservation medium during transportation or a short-time preservation process, so that the clinically ready-to-use ADSCs maintains the optimal cell activity before being applied in a human body, the effectiveness and the safety of the application of the clinical adipose-derived stem cells are improved, and clinical application and a clinical technology of the autologous adipose-derived stem cells are conveniently popularized. The preservation method provided by the invention has the advantages of conformation to theclinical standard, economy, simplicity, convenience, and capability of better maintaining cell activity. With popularization and application of the clinically ready-to-use vital preservation method for the adipose-derived stem cells, the effectiveness and the safety of the clinical application of cell products can be improved, and great economic and social benefits can be achieved.

A triple-chambered container includes: a main container body with a first chamber which has an opening at its distal end; a main barrel formed and movable longitudinally within the first chamber, the main barrel defining therein a second chamber for receiving fluids, the main barrel further having an apertured stopper at its distal end; a second barrel formed within the main barrel, the second barrel defining a third chamber for receiving fluids, the second barrel being movable longitudinally within and with respect to the main barrel, the second barrel having a distal end which is engageable and disengageable with the apertured stopper; a shaft adapted to fit within the second barrel, the shaft being movable longitudinally within and with respect to the second barrel, the shaft having a distal end which is engageable and disengageable with an aperture in the second barrel; a device for controlling engaging and disengaging of the distal end of the shaft with the aperture of the second barrel. The first chamber, the second chamber and the third chamber may be selectively moved to receive and discharge fluids with respect to one another.

The invention discloses a tissue engineering biological material for transplanting and restoring a peripheral nerve defect. The tissue engineering biological material is prepared through the following steps: firstly, preparing an acellular nerve allograft scaffold by using a chemical extraction method; then culturing an adipose derived stem cell by using fatty tissues collected by a target receptor in an early debridement; finally carrying out induction in vitro by using an exogenous schwann cell endogenus neurotrophic factor to construct a tissue engineered peripheral nerve for later use. The acellular nerve allograft scaffold subject to chemical extraction has no immunogenicity and has a network structure most similar to an organism nerve and excellent biocompatibility, and meanwhile, a stem cell is controllably induced in vitro by using the schwann cell endogenus neurotrophic factor and is differentiated into schwann-liked cells in combination with an ideal scaffold and then transplanted in vivo to become effectiveness.

The invention discloses a method for making a fully autologous tissue double-coated conformable breast prosthesis. According to the pre-estimated resection range and volume, the skin epidermis and dermis tissue, as well as subcutaneous fat tissue are obtained from the patient's own body, and then The epidermis and skin-removed adipose tissue are placed in a sterile container, placed in a water bath box at 50-60 degrees Celsius for 20-40 minutes to inactivate, and the inactivated autologous adipose tissue is trimmed and sewn into a desired shape and size. Conformal breast prosthesis content, according to the shape and size of the conformal breast prosthesis content, the inactivated skin surface tissue is sewn into the inner capsule of the conformal breast prosthesis content, and the dermis is sewn The outer capsule that forms the conformal breast implant contents. The method of the present invention can prevent the infiltration of inflammatory cells in the surrounding tissue after the prosthesis is implanted, prevent the possible inflammatory reaction of the prosthesis content, have no risk of prosthesis stabbing and rupture, and improve the toughness, elasticity, Shape maintenance and anti-extrusion ability.