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Tissue engineering biological material for transplanting and restoring peripheral nerve defect

A peripheral nerve and tissue engineering technology, applied in the biological field, can solve the problems of short half-life, difficult and long-term matching of neurotrophic factors, and achieve long-term induction and differentiation, good biocompatibility, and meet the needs of clinical treatment. Effect

Inactive Publication Date: 2015-05-13
PLA NAVY GENERAL HOSIPTAL
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Problems solved by technology

However, the half-life of neurotrophic factors is short, it is difficult to play a long-term role in the body, and it cannot match the long-term need for nerve regeneration

Method used

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Embodiment Construction

[0022] Hereinafter, the present invention will be specifically described by taking dogs as experimental subjects.

[0023] The specific technical method comprises the following process steps:

[0024] one. Preparation of decellularized nerve allografts

[0025] The sciatic nerve of the dog within 1 hour of death was used as the material. After the material was collected, the nerve was placed in PBS solution and stored at 4°C for 10 hours.

[0026] 1. Soak in sterile distilled water for 12 hours;

[0027] 2. Shake in 5% Triton X-100 solution for 12 hours to degrade and remove some cell disintegration fragments;

[0028] 3. Soak again in sterile distilled water for 3 hours to further remove cell debris;

[0029] 4. Shake in 5% sodium deoxycholate solution for 12 hours;

[0030]5. Soak the peripheral nerve material treated in step 4 in sterile distilled water again, and repeat the process of steps 1-4 twice. The four process cycles of "soaking in sterile distilled water → s...

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Abstract

The invention discloses a tissue engineering biological material for transplanting and restoring a peripheral nerve defect. The tissue engineering biological material is prepared through the following steps: firstly, preparing an acellular nerve allograft scaffold by using a chemical extraction method; then culturing an adipose derived stem cell by using fatty tissues collected by a target receptor in an early debridement; finally carrying out induction in vitro by using an exogenous schwann cell endogenus neurotrophic factor to construct a tissue engineered peripheral nerve for later use. The acellular nerve allograft scaffold subject to chemical extraction has no immunogenicity and has a network structure most similar to an organism nerve and excellent biocompatibility, and meanwhile, a stem cell is controllably induced in vitro by using the schwann cell endogenus neurotrophic factor and is differentiated into schwann-liked cells in combination with an ideal scaffold and then transplanted in vivo to become effectiveness.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to biomaterials prepared by tissue engineering methods. This tissue engineered biomaterial is used to implant into the human body to repair peripheral nerve defects in the human body. Background technique [0002] Peripheral nerves refer to all nerves other than the brain and spinal cord relative to the central nervous system. Peripheral nerve damage or loss encountered in clinical medicine generally occurs in the body, resulting in sensory and motor dysfunction in patients. Patients with peripheral nerve injuries often require multiple surgeries for treatment. In the first-stage operation, debridement, bone reduction and fixation are performed, and nerve repair is postponed. At present, the commonly used and effective method of peripheral nerve repair in clinic is autologous nerve transplantation. After weighing the pros and cons, the method is to "tear down the east wall to make up...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/074C12N11/02A61L27/38A61L27/36A61F2/02
Inventor 李威阮狄克白雪东
Owner PLA NAVY GENERAL HOSIPTAL
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