6results about How to "Non-immunogenic" patented technology

The invention discloses an oligomeric amino acid dendritic macromolecular material as well as a preparation method and application thereof, and relates to the technical field of nano materials. The general formula of the oligomeric amino acid dendritic macromolecular material is TPE-(PEG) n-(Lys) x-(Arg) y, wherein TPE is a hydrophobic core, has an aggregation-induced emission characteristic, and can enhance the self-assembly capability and the tracing function of the nano-carrier; pEG is a connecting arm, so that the water solubility and biocompatibility of the material can be improved; lys is a branch skeleton and is a main construction unit of a dendritic macromolecule; arg is a functional terminal, guanidyl of Arg has strong positive charges, electrostatic binding with negatively charged nucleic acid can be promoted, and meanwhile cellular uptake can be enhanced possibly through the membrane penetration effect. The oligomeric amino acid dendritic macromolecular material gives consideration to stability, modifiability and targeting, provides a new scheme for small nucleic acid delivery, and is expected to have far-reaching influence in the fields of precise tumor treatment, gene therapy and multi-modal diagnosis and treatment.

The present application relates to the field of medical supplies, and specifically provides a recombinant keratin injectable gel and a preparation method thereof. The injectable gel can form a recombinant keratin gel with reliable quality without adding a cross-linking agent while maintaining the mass ratio α of keratin and cysteine ​​at 5:1≤α<15:1 and adjusting to an alkaline pH value. The application of the injectable gel in medical products includes soft tissue filling, drug delivery, and cosmetic surgery. The injectable gel can also be used to promote fibroblast proliferation and migration, and promote fibroblast expression and secretion of collagen.

This invention belongs to the field of molecular biology and medical detection technology, and discloses a bladder cancer-specific nucleic acid aptamer, nucleic acid aptamer derivatives, and their applications. The nucleotide sequences of the nucleic acid aptamer are shown in SEQ ID NO: 1, 2, and 3. This invention screened three monoclonal nucleic acid aptamer DNA chains from bladder cancer tissue samples using the exponential enrichment systematic evolution (SELEX) technique and identified two target proteins that bind to these three nucleic acid aptamers. The nucleic acid aptamers specifically target the target proteins CAPRIN1 and FXR1 on the surface of bladder tumor cells. The nucleic acid aptamers obtained by this invention have advantages such as high affinity and specificity, no immunogenicity, simple chemical synthesis, stability, and ease of storage and labeling, providing a new molecular tool for the early diagnosis and targeted therapy of bladder cancer.

The invention relates to drug-loaded polypeptide hydrogel as well as a preparation method and application thereof, in particular to polypeptide hydrogel which is formed by self-assembling polypeptide containing a specific amino acid sequence and doxorubicin active drugs, the polypeptide hydrogel disclosed by the invention can realize rapid hemostasis in an operation, precise targeted drug delivery after the operation and long-acting drug sustained release, effectively reduces toxic and side effects, and has the advantages of safe and reliable components, simple preparation method, good biocompatibility and the like.

The invention relates to the technical field of biomolecular engineering, in particular to a DNA aptamer capable of specifically recognizing natural CRP and application, and the DNA aptamer capable of specifically recognizing natural CRP contains at least one of the following sequences: (1) a nucleic acid sequence as shown in SEQ ID NO: 1 and a functional derivative thereof; and (2) a nucleic acid sequence as shown in SEQ ID NO: 2 and a functional derivative thereof. The DNA aptamer provided by the invention can be highly specifically combined with natural CRP in a complex biological sample, so that a novel biological detection method is provided; besides, compared with a traditional antibody method for recognizing the natural CRP, the DNA aptamer has the advantages of low cost, no immunogenicity, high in-vivo stability and the like, and is suitable for capturing the nCRP in a complex system, so that accurate detection, purification, in-vivo imaging, diagnosis, drug delivery and the like of the nCRP are realized.

The invention discloses a recombinant humanized XVII type collagen COL1502 and a preparation method thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the protein is as shown in SEQ ID NO.2, and the coding gene sequence of the protein comprises a sequence as shown in SEQ ID NO.4 or SEQ ID NO.5. The preparation method comprises the following steps: constructing a recombinant bacterium Pichia.pastoris GS115COL1502, and carrying out fermentation culture, purification and the like, so as to obtain the recombinant humanized XVII type collagen COL1502. The protein can realize stable high-yield expression in saccharomycetes, has good biocompatibility, safety and no immunogenicity, can promote cell proliferation and migration, can be widely applied to the fields of medicines, biological materials and the like, and has a wide application prospect.