751results about How to "Non-immunogenic" patented technology

The invention relates to the field of specific molecular identification and diagnosis reagent and in particular discloses a near infrared fluorescent dye with structural formulas I and II; and the invention also discloses a near infrared molecular probe which is obtained through covalent bonding between the near infrared fluorescent dye with the structural formulas I and II and a ligand of specific molecules. The near infrared molecular probe can be used for early diagnosis of turmour diseases.

The invention belongs to the field of pharmaceutical agents, relates to a polymer micelle lyophilized agent encapsulating an insoluble antitumor drug as well as a preparation method and an application thereof. The polymer micelle lyophilized agent is prepared by carrying out molecular self-assembly on a methoxy poly(ethylene glycol) 2000-polyester block copolymer to form micelles, and then encapsulating the insoluble antitumor drug in a hydrophobic core formed by the polyester. The lyophilized agent has high encapsulation rate, high drug loading and small particle size, can significantly improve the water solubility of the insoluble drug and result in passive targeting of more antitumor drugs to concentrate in the tumor tissues, thus improving an anti-tumor treatment effect and reducing the toxic and side effects of drugs, and can be used to prepare the drugs used for the treatment of lung cancer, intestinal cancer, mammary cancer, ovarian cancer, etc. The lyophilized agent can also be quickly dissolved and dispersed to form a transparent micellar solution after water for injection, normal saline solution and the like are added, and is used for the preparation of the drugs for treating primary intestinal cell carcinoma.

The invention relates to hydrogel wound dressing for treating laser cauma and burns and scalds. The active components of the hydrogel wound dressing include an antibacterial agent, a humectant and a stabilizer, wherein the antibacterial agent inhibits adhesion and growth of the wound of laser cauma and surrounding bacteria and can kill various pathogenic bacteria at the cauma part, so as to effectively promote union of the laser cauma. The antibacterial agent adopted by the invention does not generate drug resistance, the humectant can maintain moisture of the wound, promote union of the wound surface, prevent formation of scars and alleviate wound pain. With the adoption of the stabilizer, the hydrogel maintains long-time antibacterial effectiveness. Experiments verify that the hydrogel dressing provided by the invention has the effects of effectively relieving wound pain, controlling local infection, maintaining moisture of wound skins, protecting skins, accelerating and promoting union of wound surface and wound and preventing formation of scars, and can be used for healing laser-burned skin wound surfaces (such as laser mole removal and the like) and wound surfaces of burns and scalds.

The invention relates to a tumor-targeted gene vector material and a preparation method and use thereof. In the invention, functional graphene oxide is used as a targeted transport vector of genomic molecules such as siRNA. The preparation method comprises: connecting folic acid tumor cell targeted molecules and amino-terminated polyethylene glycol by amido bonds; connecting the graphene oxide with the coupling material by amido bonds to obtain the tumor targeted functional graphene oxide; anchoring aminated molecules with an aromatic conjugated structure onto the surface of the graphene oxide through pi-pi conjugation; and finally, preparing the tumor targeted high-efficiency gene transport system by static attraction between the positively charged functional graphene oxide and the negatively charged genomic molecules. The vector material has a high targeting performance for tumor cells in which folic acid receptor is expressed more highly, and after being loaded with siRNA, the corresponding target gene level and related protein expression can be reduced. The invention provides a new method for the in-vivo targeted transportation of genes.

The invention relates to a folate-targeted reduction sensitive drug-carrying polymer nano-micelle as well as a preparation method and application thereof. The delivery carrier is prepared by taking anamphiphilic triblock copolymer PCL-ss-PEG-ss-PCL as a material, and a chemotherapeutic drug adriamycin amycin and a photosensitizer indocyanine green are entrapped in a hydrophobic core of the micelle. Besides, phospholipid DSPE-PEG-NH2 with an active group is introduced into the preparation process, the DSPE end of the phospholipid has strong hydrophobic property and is inserted into the hydrophobic PCL core of the polymer micelle, the flexible hydrophilic PEG long chain exists on the outer surface of the micelle, FA with a targeting effect is connected to a PEG active distal end of the surface of the polymer micelle, and functions of active tumor targeting and reduction response drug release are integrated. The folate-targeted reduction sensitive drug-carrying polymer nano-micelle disclosed by the invention has the advantages of being small in particle size, high in dispersion property, high in drug loading capacity and encapsulation efficiency and excellent in photothermal conversion effect, can realize reduced trigger drug release, fluorescence imaging of tumor sites, tumor targeting drug delivery and chemotherapy-photothermal combination therapy and improve the tumor inhibition effect, and has wide application prospects in the targeted combination therapy aspect of tumors.

The invention relates to a pH / reduction dual-sensitive multifunctional nano-micelle for tumor chemotherapy and photothermal combined therapy and application of the pH / reduction dual-sensitive multifunctional nano-micelle. The pH / reduction dual-sensitive multifunctional nano-micelle is characterized in that a PCL-acetal-PEG polymer with pH sensitive characteristic, a PCL-ss-PEG-ss-PCL polymer withreduction sensitive characteristic and a phospholipid DSPE-PEG-NH2 with an active group are used as raw materials and are blended to prepare a pH / reduction intelligent responsive polymer nano-micelleas a carrier; a chemotherapeutic drug and a photosensitizer coat a hydrophobic core of the micelle; a casing is made of hydrophilic PEG and has the characteristic of long circulation and space stability; a folic acid targeting group is modified on the surface of the micelle by a covalent bond; the drug loading capacity is 4 to 10 percent, and the mass ratio of the chemotherapeutic drug to the photosensitizer is 0.5 to 2; the particle size is smaller than 200 nm. The pH / reduction dual-sensitive multifunctional nano-micelle disclosed by the invention has the advantages of good dispersity, smallparticle size, better photothermal conversion effect and high drug loading capacity and encapsulation efficiency; the effect of improving tumor suppression by tumor targeted administration, fluorescence imaging of a tumor site, pH / reduction triggered drug release and chemotherapy and photothermal combined therapy can be realized; the pH / reduction dual-sensitive multifunctional nano-micelle has a broad clinical application prospect in combined targeting therapy of tumors.

The invention discloses a long-acting recombinant human follicle-stimulating hormone-Fc fusion protein (hFSH-Fc) and a preparation method thereof. The hFSH-Fc is a dimerization fusion protein. An amino acid sequence of the hFSH-Fc comprises an hFSHbeta subunit, CTP (carboxy-terminal peptide), an hFSHalpha subunit, a flexible peptide linker and a human IgG (immunoglobulin G) Fc variant from the N terminal to the C terminal in sequence. The hFSH-Fc has longer half-life in vivo and smaller side effects than existing hFSH. The invention also relates to an application of a recombinant hFSH-Fc composition to preparation of drugs for treating and / or preventing infertility.

The invention relates to a fusion protein of a human glucagon-like peptide -1(hGLP-1) analogues and the preparation method for the fusion protein, wherein, the fusion protein is a pro-drug with high biological stability and long half-life in vivo that releases active GLP-1 molecule after being degraded by enzyme in vivo and then brings into playing the pharmacological action. The scope of the invention extends to the treatment application of the products made by the production technique. The fusion protein can be used for treating and preventing the diseases or disorders relating the GLP-1 activity, in particular to the 2 Diabetes Mellitus. The fusion protein of a human glucagon-like peptide -1(hGLP-1) analogues and the preparation method for the fusion protein relates to the biotechnological field, with the fusion protein prepared by the gene engineering method, which has the advantages of much lower production cost than the chemical synthetic method, simple operation, and easy acquisition of raw material, and has possibility for commercial production.

The present invention discloses a nucleic acid aptamer, wherein the sequence of the nucleic acid aptamer comprises a DNA segment represented by any one sequence selected from a sequence 1, a sequence 2 and a sequence 3. The nucleic acid aptamer can further be various similar sequences with high homology or a derivative obtained from the sequence of the present invention. The invention further discloses a nucleic acid aptamer screening method, which comprises: synthesizing a random single-stranded DNA library and primers, carrying out SELEX screening, carrying out PCR amplification of library, preparing a DNA single strand library, and finally carrying out repeated screening, negative screening and multi-round screening to obtain the nucleic acid aptamer. The nucleic acid aptamer and the derivative thereof can be used in recognition of the prostate cancer cell strain PC-3 or preparation of kits, molecular probes and targeted mediums for prostate cancer detection, and can further be used in design and preparation of prostate cancer treatment drugs. Compared with the protein antibody, the nucleic acid aptamer of the present invention has advantages of high affinity, high specificity, no immunogenicity, capability of being chemically synthesized, small molecular weight, stability, easy storage, easy labeling and the like.

A preparation method of water soluble chitosan group stanch wound sponge, which is characterized in dissolving water soluble chitosan derivatives in water, preparing 1%-10% weight percentage concentration of uniform colloid solution; subpackaging the colloid solution in mould, freezing in vacuum freeze drier at -20--60 DEG C, vacuum freeze drying 24-60 hours. The water soluble chitosan group stanch wound sponge of the invention is used for stanching and boosting wound healing of clinical operation, has advantages of convenient using, great mount of absorption solution, firm adhesion, better exerting function locally; selected material absorb water gradually to form gel in physiology pH condition during preparation, products have good local pasting property, exert stanching and boosting wound healing function so much the better; and products without immunogenicity in body, short degradation time, good compatibility with living body, better safety performance of clinic.

A bone tissue regeneration guiding membrane. The invention employs an antigen-extracted mammal tissue membrane, which has a double layer structure of a compact layer and a loosening layer. The compact layer is formed by tightly arranged collagen fibers, and an external side of the compact layer is composited with a collagen coating containing active polypeptides; the loosening layer is formed by interlaced collagen fibers and is composited with ossification active factors. The prepared guiding membrane can prevent soft tissue from evolving and promote new bone formation, has good biological compatibility, hydrophilism and bone induction capability, and can be completely degraded in body without generation of harmful substances. Besides, the guiding membrane has an effect of isolating cell evolving, a characteristic of slow degradation and a longer retaining time in body than a current guiding membrane prepared from natural high-molecular polymer, and can provide enough time and space for new bone growth. The method of the invention is simple, and raw materials have wide sources and low prices, so that the method is suitable for large-scale industrial production and beneficial to lowering medical costs and mitigating patient burdens.

The invention relates to hyperbranched polyamino acid. The chemical composition is as follows: in the formula of (Lys-)a-(-Arg-)b-(-His-)c-(-X)d, Lys, Arg and His are respectively lysine, arginine and histidine, X is one of or a plurality of any amino acids except for the amino acids above, wherein a is not less than 0.2 and not more than 0.95, b is not less than 0 and not more than 0.8, c is not less than 0 and not more than 0.8, d is not less than 0 and not more than 0.3, and the sum of a, b, c and d is 1. The preparation method is as follows: uniformly mixing lysine or lysine salt with other amino acids or the amino acid salts contained in the hyperbranched polyamino acid to react for 2-64h at the temperature of 130-180 DEG C and then obtain yellow solid, and finally purifying the coarse product with a precipitation method to obtain the hyperbranched polyamino acid. The hyperbranched polyamino acid obtained in the invention can be used as nonviral gene carrier for the transduction of huamn or animal cell gene, and the hyperbranched polyamino acid nonviral gene carrier provided by the invention is reasonable in design, simple in preparation method, high in transfection efficiency, low cytotoxicity and favorable transduction efficiency in the presence of blood serum.

The invention discloses chitosan-hydroxylapatite in-situ loaded icariin composite microspheres and a preparation method thereof. The preparation method is characterized in that a micro-capsule forming device is adopted, chitosan is used as an organic substrate, and soluble calcium salt and soluble phosphate are used as precursors of inorganic phase nanometer hydroxylapatite; the in-situ crystal induction effects of hydroxyl radicals and amino radicals in the chitosan on the hydroxylapatite are used for adjusting and controlling the in-site heterogeneous nucleation crystallization of an inorganic mineral on the organic substrate; and meanwhile, a traditional Chinese medicine, namely icariin which has the effect of promoting proliferation and differentiation on osteoblast is compounded, and the chitosan-hydroxylapatite in-situ loaded icariin composite microspheres are prepared through in-situ simulation. The chitosan-hydroxylapatite in-situ loaded icariin composite microspheres are bone filling materials, have good biocompatibility and biodegradability and are expected to serve as repair materials for bone tissue defects to be widely applied in clinic; the preparation processes are simple and easy; and the method is mild.

A composition for nursing the vagina and treating the gynopathic inflammation such as vaginitis contains the chitosan or its cationic derivative (0.1-99 Wt%). Its preparing process is also disclosed.

The invention discloses a wild antheraea pernyi silk fibroin microsphere and a preparation method of the microsphere. The preparation method comprises the steps of degumming and dissolving tussah silk; subjecting the above processed tussah silk to dialysis treatment to obtain a solution of the wild antheraea pernyi silk fibroin; adjusting the concentration of the solution to 10-100mg / ml; adding in a citric acid solution or an acetic acid buffer solution at the temperature of 10-60 DEG C to adjust the pH value to 3-6; subjecting the resulting solution to ultrasonic oscillation and stirring treatment to obtain a suspension of the wild antheraea pernyi silk fibroin microsphere; centrifugalizing, freezing and drying the suspension to obtain the biodegradable wild antheraea pernyi silk fibroin microsphere, the diameter of which is 0.1-10mu m. The preparation method of the invention prepares the wild antheraea pernyi silk fibroin microsphere by means of in-situ self-assembly generation and is simple in process procedures; the prepared microsphere is uniform in size distribution, keeps the RGD sequence of the wild antheraea pernyi silk fibroin, promotes the cell recognition of the microsphere, improves the targeting property and bioavailability of drugs, can function as a carrier with bioactive substance to carry an enzyme drug, a nucleic acid drug, a polypeptide drug, a protein drug and the like, and can be applied to diagnosing and treating diseases and the like.

This invention describes a method whereby human autoimmune antibodies are used as carrier compounds to deliver imaging agents and pharmaceutical drugs to the tumor in the human patient. These autoantibodies have the propensity to localize in necrotic areas of tumors but not in healthy normal tissues. By combining various pharmaceutical agents with these carrier proteins it is possible to localize these agents within the necrotic areas of tumors in cancer patients. The carrier proteins may be combined with a variety of imaging agents for detection and diagnosis of tumors, and / or with a variety of radioactive or cytotoxic compounds for cancer treatment.

The invention discloses a folic acid-modified O-carboxymethyl chitosan-deoxycholic acid complex which is an active-targeting nano-micelle carrier material prepared by using folic acid, O-carboxymethyl chitosan and deoxycholic acid as raw materials; O-carboxymethyl chitosan is used as a carrier material; deoxycholic acid is used for hydrophobicity reconstruction; folic acid is used for surface modification; and folic acid-mediated tumor tissue-targeting polymer micelle is prepared. Drug-loaded nano-micelle is prepared by a self-assembly method with paclitaxel as a model drug; experiment demonstrates that the drug-loaded nano-micelle has high drug load and encapsulation efficiency, has sustained release effect, is intaken into tumor cells through a folic acid acceptor approach, can increase the distribution of the drug in tumor tissue, thus improves curative effect, reduces toxic and side effect, and reaches the purpose of targeting treatment. The invention provides an ideal and novel drug carrier and preparation form for hard-soluble antitumor drugs.

The invention discloses clinic-level adipose-derived stem cell preparation and storage methods. Mesenchymal stem cell culture of a fat tissue source is performed by adopting a serum-free complete medium without animal source protein pollution, safety and effectiveness are realized, immunogenicity is avoided, the rate of increase of ADSCs can be better maintained, a plateau period is prolonged, an expansion multiple of each generation of cells is greatly improved, and the clinic treatment number is ensured; the serum-free complete medium is applied to preparation of clinic-level ADSCs for the first time; a fat tissue is collected from the abdomen and can be taken discontinuously repeatedly, thus avoiding damage to the adipose-derived stem cells in a liposuction process; the ADSCs are prepared by using a wall adherence method, thus increasing the primary cell yield and lowering the cost; an adopted novel serum-free cell frozen preservation solution meets a clinical application standard, reduces the use amount of DMSO, does not obviously influence the motility rate, phenotype and doubling time of the ADSCs, can be directly used in clinic infusion after reviving, is convenient to transport, and ensures the safety of clinic treatment.

A compound Cu(DDC)2 with relatively high tumor-inhibiting activity can be used for treating cancers such as melanoma, breast cancer, lung cancer, liver cancer, and the like. The treatment effect is broad, and the compound has certain selective killing effect against tumor cells. The invention relates to an Cu(DDC)2 injection protein nano-particle preparation used for treating tumor, and a preparation method thereof. Therefore, problems of poor water-solubility and difficulty in intravenous administration of Cu(DDC)2 are solved. According to the invention, with an ultrasonic method, a high-pressure homogenization method, and a micro-jet method, the Cu(DDC)2 injection protein nano-particles are prepared. The Cu(DDC)2 protein nano-particles are prepared from the components that (Cu(DDC)2 + organic solvent) :(protein + water) = 1:2-1:30, wherein the specification of Cu(DDC)2 is 0.01-5mg / mL, the specification of protein is 0.05-25% (w / v), and a ratio of organic solvent to water is 1:2-1:30. Proteins such as human serum albumin (HSA), bovine serum albumin (BSA), hydrophobic protein, glycoprotein, and lipoprotein or polypeptide is adopted as a carrier and a stabilizer for coating medicine particles, such that the particles have good biocompatibility and high security. Cu(DDC)2 tumor-inhibiting treatment effect is improved, and adverse reaction is reduced.

The invention discloses a dressed millimicron liposome of resveratrol, liposome comprises resveratrol, lecithin or soybean lecithin, cholesterin 50-85%, chloridized chitosan 0.5-1% and auxiliary materials 5-40%. The dressed liposome has the advantages of better adaptability for patients and simpler preparing process.

The invention belongs to the technical field of preparation of biomedical high molecular polymer materials and discloses a cholesterol modified amphiphilic pH response pennicuius copolymer and a preparation method and a micelle system prepared based thereof. The copolymer has a structure shown in the formula I in the specification, wherein x is 10-36, y is 15-40 and z is 8-30. The copolymer is obtained by virtue of irregular copolymerization of a hydrophilic block hydroxyethyl methylacrylate, hydrophobic cholesterol and pH response block methacrylic acid N, N-diethyl aminoethyl combined with hydrophilic poly(ethylene glycol) methyl ether methacrylate. The copolymer is dissolved in a solvent to obtain a nanoscale micelle system, wherein the inner layer is a cholesterol modified hydrophobic chain segment, the middle layer is a pH response chain segment and the shell is a hydrophilic chain segment, so that the function of high entrapment performance, stable existence of a neutral condition and quick release in a weak acidic condition is achieved. By adjusting the proportions of the blocks in the polymer, the release rates of medicines can be regulated to satisfy the release requirements on different drugs.

The invention discloses hydrochloric acid Fasudil liposome injection and new application thereof. The injection mainly comprises the following components according to parts by weight: 1 part of hydrochloric acid Fasudil, 2 to 20 parts of phospholipid, 0.5 to 10 parts of cholesterol and 1 to 8 parts of polysorbate 80. Simultaneously, the hydrochloric acid Fasudil liposome injection also can be used for treating vertebral artery type cervical spondylosis.

The invention discloses a preparation method of a tumor targeted nanoparticle carrier co-loaded with breast cancer chemotherapeutic drug MTDH siRNA. A PEI-PLGA (polyethyleneimine-poly(lactic-co-glycolic acid)) polymer is dissolved in dichloromethane, deionized water is added, the solution is subjected to ultrasonic crushing and emulsified into a homogenous emulsion, vinyl alcohol, hydrophobic taxol and dichloromethane are mixed and added to the emulsion, the mixture is subjected to ultrasonic crushing and emulsified, the emulsion is evaporated, a nanoparticle suspension is obtained, nanoparticle cores coated with taxol are prepared from the nanoparticle suspension, and then, bleaching, stirring and centrifugation are performed. The breast cancer chemotherapeutic drug and nucleic acid are carried into breast cancer tumor cells with high-expression MTDH genes to inhibit cell proliferation and have significant in-vitro and in-vivo tumor targeting. The carrier has clear anti-tumor effects,used carrier materials have high safety, and the carrier has good biocompatibility and biodegradability and has no biotoxicity or immunogenicity. The preparation process is simple, easy to operate, time-saving, energy-saving and suitable for mass production.

The invention relates to a hernia patch solid-supported with antibiotics and a preparation method. The antibiotic hernia patch solid-supported with drug-loaded microspheres comprises drug-loaded microspheres, a patch, and a supporting agent. The sandwich-type antibiotic hernia patch comprises a patch layer, a drug layer, and a film layer. The antibiotic hernia patch solid-supported with drug-loaded microspheres is prepared by solid-supporting the drug-loaded microspheres onto the surface of the patch material by the supporting agent. The drug-loaded microspheres are loaded with water-soluble drugs by a solvent evaporation method. The sandwich-type antibiotic hernia patch is prepared by sandwiching the drug layer between the patch layer and the film layer through a layer-by-layer superposition principle. The antibiotic hernia patch of the invention has a simple and practical preparation method, is suitable for different clinical medication requirements, has obvious slow release performance, has significant antibiotic effect in rats, solves the problem of acute and delayed infection after herniorrhaphy, has good market prospects, and is worthy of popularization and utilization.

The invention discloses a nucleic acid aptamer with a sequence containing DNA (deoxyribonucleic acid) segments shown by any of a sequence 1 and a sequence 2. The nucleic acid aptamer can also be derivatives obtained by various similar sequences high in homology or the sequence. The invention further discloses a screening method of the nucleic acid aptamer. The method includes: synthesizing a random single-strand DNA library and a primer, performing Cell-SELEX screening and PCR (polymerase chain reaction) library amplification, preparing a DNA single-strand library, and obtaining the nucleic acid aptamer through repeated screening, negative screening and several rounds of screening. The nucleic acid aptamer and derivatives thereof can be applied to recognizing human biliary duct carcinoma cell line QBC-939 or preparing a reagent box for detecting the human biliary duct carcinoma cell line QBC-939, has affinity and specificity higher than anti-keratin antibodies, and is free of immunogenicity, capable of being chemically synthesized, small in molecular weight, stable, easy to store and mark, and the like.

The invention discloses a preparation method of composite styptic powder. The composite styptic powder is obtained by emulsifying and crosslinking carboxymethyl chitosan and sodium carboxymethylcellulose. Specifically, the preparation method comprises the steps of firstly crosslinking starch, then blending the crosslinked starch with carboxymethyl chitosan and sodium carboxymethylcellulose, emulsifying, and crosslinking again to obtain the composite styptic powder. The grain size of the composite styptic powder is 30-100 Mu m, the adsorption property is good, the surface is rippled, the safety is high, and the styptic property is good.

The invention discloses a liposome solid preparation of a losartan potassium hydrochlorothiazide pharmaceutical composition and a preparation method thereof. In the invention, active components of losartan potassium and hydrochlorothiazide and specific combined hydrogenated yolk lecithin, cholesterol and poloxamer 188 are prepared into a liposome which is then mixed with other pharmaceutical accessories to prepare the solid preparation, thereby greatly improving the pharmaceutical stability and bioavailability and having stable and lasting effect, small side effect and obvious curative effect.

The invention discloses a redox-sensitive core-crosslinked Pulullan nano particle having dual-targeting property and a preparation method thereof, preparation of a drug carrying nano particle with the compound as a carrier, and in-vitro releasing researching of the drug carrying nano particle. The method includes the steps of: 1) performing esterification reaction to lipoic acid and Pulullan to convert the water-soluble Pulullan into a hydrophobic polymer, which is beneficial to preparation of nano particles and entrapment of a hydrophobic medicine; 2) conjugating the polymer with folic acid to form a folic acid-Pulullan-lipoic acid conjugate, and performing crosslinking to inner cores of the nano particles in DTT in catalytic quantity to prepare stable and reversible folic acid-Pulullan-lipoic acid (FA-Pull-LA) nano particles having dual-targeting property. The drug carrying nano particle is prepared with paclitaxel as a model medicine in a dialysis manner, wherein stability and reduction sensitivity of the drug carrying nano particle are inspected through an in-vitro releasing test. A result proves that the dialysis method for preparing the FA-Pull-LA nano particles is simple and has good repeatability, is easy to achieve expanded production and has high drug carrying rate. The nano particle is stable in vitro and can quickly release the drug in an intracellular reductive environment.

The invention discloses an instant collagen lyophilized ball which comprises the following components in percentage by mass: 14-20% of collagen, 10-15% of trehalose, 10-15% of pullulan, 30-56% of mannitol and 10-20% of sterile injection water. The invention further discloses a preparation method of the instant collagen lyophilized ball. The instant collagen lyophilized ball has the repairing function; the prepared collagen lyophilized ball has very strong hydrophilicity, can be quickly and uniformly dissolved in the use moment, and can be used for reparation and maintenance of skin suffered from medical plastic surgeries of laser, photon, refrigeration, high frequency, grinding, face-lifting and the like; the instant collagen lyophilized ball can be used for treating skin damages, caused by acne or trauma, of depressed scars, couperose skin symptom, skin atrophy, sun burn, heat injury and the like. The instant collagen lyophilized ball prepared according to the method has very strong hydrophilicity, can be uniformly dissolved instantly when in use, is more convenient and fast to use and long in guarantee period, can be stored at the room temperature, and is very convenient to store.