749results about How to "Non-immunogenic" patented technology

The invention relates to the field of specific molecular identification and diagnosis reagent and in particular discloses a near infrared fluorescent dye with structural formulas I and II; and the invention also discloses a near infrared molecular probe which is obtained through covalent bonding between the near infrared fluorescent dye with the structural formulas I and II and a ligand of specific molecules. The near infrared molecular probe can be used for early diagnosis of turmour diseases.

The invention belongs to the field of pharmaceutical agents, relates to a polymer micelle lyophilized agent encapsulating an insoluble antitumor drug as well as a preparation method and an application thereof. The polymer micelle lyophilized agent is prepared by carrying out molecular self-assembly on a methoxy poly(ethylene glycol) 2000-polyester block copolymer to form micelles, and then encapsulating the insoluble antitumor drug in a hydrophobic core formed by the polyester. The lyophilized agent has high encapsulation rate, high drug loading and small particle size, can significantly improve the water solubility of the insoluble drug and result in passive targeting of more antitumor drugs to concentrate in the tumor tissues, thus improving an anti-tumor treatment effect and reducing the toxic and side effects of drugs, and can be used to prepare the drugs used for the treatment of lung cancer, intestinal cancer, mammary cancer, ovarian cancer, etc. The lyophilized agent can also be quickly dissolved and dispersed to form a transparent micellar solution after water for injection, normal saline solution and the like are added, and is used for the preparation of the drugs for treating primary intestinal cell carcinoma.

The invention relates to a tumor-targeted gene vector material and a preparation method and use thereof. In the invention, functional graphene oxide is used as a targeted transport vector of genomic molecules such as siRNA. The preparation method comprises: connecting folic acid tumor cell targeted molecules and amino-terminated polyethylene glycol by amido bonds; connecting the graphene oxide with the coupling material by amido bonds to obtain the tumor targeted functional graphene oxide; anchoring aminated molecules with an aromatic conjugated structure onto the surface of the graphene oxide through pi-pi conjugation; and finally, preparing the tumor targeted high-efficiency gene transport system by static attraction between the positively charged functional graphene oxide and the negatively charged genomic molecules. The vector material has a high targeting performance for tumor cells in which folic acid receptor is expressed more highly, and after being loaded with siRNA, the corresponding target gene level and related protein expression can be reduced. The invention provides a new method for the in-vivo targeted transportation of genes.

The invention discloses a long-acting recombinant human follicle-stimulating hormone-Fc fusion protein (hFSH-Fc) and a preparation method thereof. The hFSH-Fc is a dimerization fusion protein. An amino acid sequence of the hFSH-Fc comprises an hFSHbeta subunit, CTP (carboxy-terminal peptide), an hFSHalpha subunit, a flexible peptide linker and a human IgG (immunoglobulin G) Fc variant from the N terminal to the C terminal in sequence. The hFSH-Fc has longer half-life in vivo and smaller side effects than existing hFSH. The invention also relates to an application of a recombinant hFSH-Fc composition to preparation of drugs for treating and/or preventing infertility.

The present invention discloses a nucleic acid aptamer, wherein the sequence of the nucleic acid aptamer comprises a DNA segment represented by any one sequence selected from a sequence 1, a sequence 2 and a sequence 3. The nucleic acid aptamer can further be various similar sequences with high homology or a derivative obtained from the sequence of the present invention. The invention further discloses a nucleic acid aptamer screening method, which comprises: synthesizing a random single-stranded DNA library and primers, carrying out SELEX screening, carrying out PCR amplification of library, preparing a DNA single strand library, and finally carrying out repeated screening, negative screening and multi-round screening to obtain the nucleic acid aptamer. The nucleic acid aptamer and the derivative thereof can be used in recognition of the prostate cancer cell strain PC-3 or preparation of kits, molecular probes and targeted mediums for prostate cancer detection, and can further be used in design and preparation of prostate cancer treatment drugs. Compared with the protein antibody, the nucleic acid aptamer of the present invention has advantages of high affinity, high specificity, no immunogenicity, capability of being chemically synthesized, small molecular weight, stability, easy storage, easy labeling and the like.

A preparation method of water soluble chitosan group stanch wound sponge, which is characterized in dissolving water soluble chitosan derivatives in water, preparing 1%-10% weight percentage concentration of uniform colloid solution; subpackaging the colloid solution in mould, freezing in vacuum freeze drier at -20--60 DEG C, vacuum freeze drying 24-60 hours. The water soluble chitosan group stanch wound sponge of the invention is used for stanching and boosting wound healing of clinical operation, has advantages of convenient using, great mount of absorption solution, firm adhesion, better exerting function locally; selected material absorb water gradually to form gel in physiology pH condition during preparation, products have good local pasting property, exert stanching and boosting wound healing function so much the better; and products without immunogenicity in body, short degradation time, good compatibility with living body, better safety performance of clinic.

A bone tissue regeneration guiding membrane. The invention employs an antigen-extracted mammal tissue membrane, which has a double layer structure of a compact layer and a loosening layer. The compact layer is formed by tightly arranged collagen fibers, and an external side of the compact layer is composited with a collagen coating containing active polypeptides; the loosening layer is formed by interlaced collagen fibers and is composited with ossification active factors. The prepared guiding membrane can prevent soft tissue from evolving and promote new bone formation, has good biological compatibility, hydrophilism and bone induction capability, and can be completely degraded in body without generation of harmful substances. Besides, the guiding membrane has an effect of isolating cell evolving, a characteristic of slow degradation and a longer retaining time in body than a current guiding membrane prepared from natural high-molecular polymer, and can provide enough time and space for new bone growth. The method of the invention is simple, and raw materials have wide sources and low prices, so that the method is suitable for large-scale industrial production and beneficial to lowering medical costs and mitigating patient burdens.

The invention discloses a folic acid-modified O-carboxymethyl chitosan-deoxycholic acid complex which is an active-targeting nano-micelle carrier material prepared by using folic acid, O-carboxymethyl chitosan and deoxycholic acid as raw materials; O-carboxymethyl chitosan is used as a carrier material; deoxycholic acid is used for hydrophobicity reconstruction; folic acid is used for surface modification; and folic acid-mediated tumor tissue-targeting polymer micelle is prepared. Drug-loaded nano-micelle is prepared by a self-assembly method with paclitaxel as a model drug; experiment demonstrates that the drug-loaded nano-micelle has high drug load and encapsulation efficiency, has sustained release effect, is intaken into tumor cells through a folic acid acceptor approach, can increase the distribution of the drug in tumor tissue, thus improves curative effect, reduces toxic and side effect, and reaches the purpose of targeting treatment. The invention provides an ideal and novel drug carrier and preparation form for hard-soluble antitumor drugs.

The invention discloses clinic-level adipose-derived stem cell preparation and storage methods. Mesenchymal stem cell culture of a fat tissue source is performed by adopting a serum-free complete medium without animal source protein pollution, safety and effectiveness are realized, immunogenicity is avoided, the rate of increase of ADSCs can be better maintained, a plateau period is prolonged, an expansion multiple of each generation of cells is greatly improved, and the clinic treatment number is ensured; the serum-free complete medium is applied to preparation of clinic-level ADSCs for the first time; a fat tissue is collected from the abdomen and can be taken discontinuously repeatedly, thus avoiding damage to the adipose-derived stem cells in a liposuction process; the ADSCs are prepared by using a wall adherence method, thus increasing the primary cell yield and lowering the cost; an adopted novel serum-free cell frozen preservation solution meets a clinical application standard, reduces the use amount of DMSO, does not obviously influence the motility rate, phenotype and doubling time of the ADSCs, can be directly used in clinic infusion after reviving, is convenient to transport, and ensures the safety of clinic treatment.

A compound Cu(DDC)2 with relatively high tumor-inhibiting activity can be used for treating cancers such as melanoma, breast cancer, lung cancer, liver cancer, and the like. The treatment effect is broad, and the compound has certain selective killing effect against tumor cells. The invention relates to an Cu(DDC)2 injection protein nano-particle preparation used for treating tumor, and a preparation method thereof. Therefore, problems of poor water-solubility and difficulty in intravenous administration of Cu(DDC)2 are solved. According to the invention, with an ultrasonic method, a high-pressure homogenization method, and a micro-jet method, the Cu(DDC)2 injection protein nano-particles are prepared. The Cu(DDC)2 protein nano-particles are prepared from the components that (Cu(DDC)2 + organic solvent) :(protein + water) = 1:2-1:30, wherein the specification of Cu(DDC)2 is 0.01-5mg/mL, the specification of protein is 0.05-25% (w/v), and a ratio of organic solvent to water is 1:2-1:30. Proteins such as human serum albumin (HSA), bovine serum albumin (BSA), hydrophobic protein, glycoprotein, and lipoprotein or polypeptide is adopted as a carrier and a stabilizer for coating medicine particles, such that the particles have good biocompatibility and high security. Cu(DDC)2 tumor-inhibiting treatment effect is improved, and adverse reaction is reduced.

The invention discloses a dressed millimicron liposome of resveratrol, liposome comprises resveratrol, lecithin or soybean lecithin, cholesterin 50-85%, chloridized chitosan 0.5-1% and auxiliary materials 5-40%. The dressed liposome has the advantages of better adaptability for patients and simpler preparing process.

The invention belongs to the technical field of preparation of biomedical high molecular polymer materials and discloses a cholesterol modified amphiphilic pH response pennicuius copolymer and a preparation method and a micelle system prepared based thereof. The copolymer has a structure shown in the formula I in the specification, wherein x is 10-36, y is 15-40 and z is 8-30. The copolymer is obtained by virtue of irregular copolymerization of a hydrophilic block hydroxyethyl methylacrylate, hydrophobic cholesterol and pH response block methacrylic acid N, N-diethyl aminoethyl combined with hydrophilic poly(ethylene glycol) methyl ether methacrylate. The copolymer is dissolved in a solvent to obtain a nanoscale micelle system, wherein the inner layer is a cholesterol modified hydrophobic chain segment, the middle layer is a pH response chain segment and the shell is a hydrophilic chain segment, so that the function of high entrapment performance, stable existence of a neutral condition and quick release in a weak acidic condition is achieved. By adjusting the proportions of the blocks in the polymer, the release rates of medicines can be regulated to satisfy the release requirements on different drugs.

The invention discloses hydrochloric acid Fasudil liposome injection and new application thereof. The injection mainly comprises the following components according to parts by weight: 1 part of hydrochloric acid Fasudil, 2 to 20 parts of phospholipid, 0.5 to 10 parts of cholesterol and 1 to 8 parts of polysorbate 80. Simultaneously, the hydrochloric acid Fasudil liposome injection also can be used for treating vertebral artery type cervical spondylosis.

The invention relates to a hernia patch solid-supported with antibiotics and a preparation method. The antibiotic hernia patch solid-supported with drug-loaded microspheres comprises drug-loaded microspheres, a patch, and a supporting agent. The sandwich-type antibiotic hernia patch comprises a patch layer, a drug layer, and a film layer. The antibiotic hernia patch solid-supported with drug-loaded microspheres is prepared by solid-supporting the drug-loaded microspheres onto the surface of the patch material by the supporting agent. The drug-loaded microspheres are loaded with water-soluble drugs by a solvent evaporation method. The sandwich-type antibiotic hernia patch is prepared by sandwiching the drug layer between the patch layer and the film layer through a layer-by-layer superposition principle. The antibiotic hernia patch of the invention has a simple and practical preparation method, is suitable for different clinical medication requirements, has obvious slow release performance, has significant antibiotic effect in rats, solves the problem of acute and delayed infection after herniorrhaphy, has good market prospects, and is worthy of popularization and utilization.

The invention discloses a liposome solid preparation of a losartan potassium hydrochlorothiazide pharmaceutical composition and a preparation method thereof. In the invention, active components of losartan potassium and hydrochlorothiazide and specific combined hydrogenated yolk lecithin, cholesterol and poloxamer 188 are prepared into a liposome which is then mixed with other pharmaceutical accessories to prepare the solid preparation, thereby greatly improving the pharmaceutical stability and bioavailability and having stable and lasting effect, small side effect and obvious curative effect.

The invention discloses a redox-sensitive core-crosslinked Pulullan nano particle having dual-targeting property and a preparation method thereof, preparation of a drug carrying nano particle with the compound as a carrier, and in-vitro releasing researching of the drug carrying nano particle. The method includes the steps of: 1) performing esterification reaction to lipoic acid and Pulullan to convert the water-soluble Pulullan into a hydrophobic polymer, which is beneficial to preparation of nano particles and entrapment of a hydrophobic medicine; 2) conjugating the polymer with folic acid to form a folic acid-Pulullan-lipoic acid conjugate, and performing crosslinking to inner cores of the nano particles in DTT in catalytic quantity to prepare stable and reversible folic acid-Pulullan-lipoic acid (FA-Pull-LA) nano particles having dual-targeting property. The drug carrying nano particle is prepared with paclitaxel as a model medicine in a dialysis manner, wherein stability and reduction sensitivity of the drug carrying nano particle are inspected through an in-vitro releasing test. A result proves that the dialysis method for preparing the FA-Pull-LA nano particles is simple and has good repeatability, is easy to achieve expanded production and has high drug carrying rate. The nano particle is stable in vitro and can quickly release the drug in an intracellular reductive environment.

The invention discloses an instant collagen lyophilized ball which comprises the following components in percentage by mass: 14-20% of collagen, 10-15% of trehalose, 10-15% of pullulan, 30-56% of mannitol and 10-20% of sterile injection water. The invention further discloses a preparation method of the instant collagen lyophilized ball. The instant collagen lyophilized ball has the repairing function; the prepared collagen lyophilized ball has very strong hydrophilicity, can be quickly and uniformly dissolved in the use moment, and can be used for reparation and maintenance of skin suffered from medical plastic surgeries of laser, photon, refrigeration, high frequency, grinding, face-lifting and the like; the instant collagen lyophilized ball can be used for treating skin damages, caused by acne or trauma, of depressed scars, couperose skin symptom, skin atrophy, sun burn, heat injury and the like. The instant collagen lyophilized ball prepared according to the method has very strong hydrophilicity, can be uniformly dissolved instantly when in use, is more convenient and fast to use and long in guarantee period, can be stored at the room temperature, and is very convenient to store.