Application of autologous adipose-derived mesenchymal stem cells in preparation of medicines for treating joint diseases

A technology for mesenchymal stem cells and joint diseases, which is used in the extraction of autologous adipose-derived mesenchymal stem cells and the treatment of joint diseases. It can solve the problems of losing the therapeutic effect of mesenchymal stem cells, mutation, mutation, etc., so as to avoid immune rejection and disease cross-infection. , the effect of good differentiation potential

Inactive Publication Date: 2014-02-05
南京优而生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These allogeneic mesenchymal stem cells must go through processes such as cell cryopreservation recovery, culture, expansion and passaging, which change the quality of the cells, thus losing the therapeutic effect of mesenchymal stem cells, and even produce serious adverse consequences such as mutations and mutations.

Method used

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  • Application of autologous adipose-derived mesenchymal stem cells in preparation of medicines for treating joint diseases
  • Application of autologous adipose-derived mesenchymal stem cells in preparation of medicines for treating joint diseases
  • Application of autologous adipose-derived mesenchymal stem cells in preparation of medicines for treating joint diseases

Examples

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Embodiment 1

[0047] 1. A 63-year-old male patient suffered from traumatic knee arthritis for 15 years. Under the condition of local anesthesia, collect 100mL of fat from the lower abdomen of the patient, and transfer it to a 500mL closed sterile container; add 200mL of PBS buffer at 37 o C. Shake the incubator on an air bath at 100 rpm, repeat three times of rinsing, each time for 30 minutes, and use a pipette to suck out the liquid in the lower layer of the tissue. Do this until the sucked liquid is clear;

[0048] 2. Add adipose tissue digestion solution containing 0.175mg / mL type I collagenase of the same volume as the tissue, the total amount at this time is 200mL;

[0049] 3. Seal, place in a constant temperature shaker at 37°C, and digest with shaking at 200rpm for 30 minutes;

[0050] 4. Add 11mL FBS (final concentration: 5%) to stop the digestion, transfer the digested fat into 50mL centrifuge tubes in 4 parts, and centrifuge at room temperature at 500 x g for 15 minutes. At this...

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Abstract

The invention relates to an application of autologous adipose-derived mesenchymal stem cells in preparation of medicines for treating joint diseases. The application is characterized in that adipose-derived mesenchymal stem cells (Ad-MSCs) separated and extracted from autologous adipose tissues of the patient are injected to the diseased articular cavity in a large dose once. The adopted mesenchymal stem cells have immunophenotyping and representing biological marks of Ad-MSC through flow cytometry authentication, and can be directionally induced and differentiated to osteoblast and chondrocyte. Primary cells are directly injected to the articular cavity of the diseased joint to treat various joint diseases. With the adoption of the autologous adipose-derived mesenchymal stem cells as the primary cells, the cells have better differentiative potential and the ability of inhibiting inflammation and immunoregulation ability. The adipose-derived mesenchymal stem cell transplant provides a novel effective means for treating osteoarticular diseases. The method is simple and feasible, and the quantity of cells collected is great and the trauma is small. The autologous mesenchymal stem cells of the patient are applied without invading related ethics, so that the risk of immunological rejection, infectious diseases and the like is avoided.

Description

technical field [0001] The invention relates to the field of cell transplantation therapy, in particular to a method for extracting autologous adipose-derived mesenchymal stem cells and treating joint diseases. Background technique [0002] Arthritis is a common chronic disease, common arthritis includes osteoarthritis such as senile degenerative arthritis, sports injury arthritis; rheumatoid arthritis, causing joint swelling and pain, limited mobility, seriously affecting the Quality of Life. According to statistics, there are more than 100 million arthritis patients in my country at present. Half of people over the age of 50 suffer from osteoarthritis; 90% of women and 80% of men over 65 years of age suffer from osteoarthritis; the prevalence of rheumatoid arthritis in my country is 0.34% to 0.36%. At present, there is no specific treatment method. In the late stage, joint deformation may develop, requiring surgical treatment or even joint replacement. [0003] Stem cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/34A61P19/02A61P29/00A61P19/08A61K35/28
Inventor 金星亮郑菊芬杨五宁刘洁
Owner 南京优而生物科技发展有限公司
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