The invention discloses traditional Chinese medicine coffee with functions of improving immunity and relieving fatigue, and a preparation method thereof. The traditional Chinese medicine coffee comprises the following components: sword beans, hawthorns, sealwort, longans, medlar, red dates, momordica grosvenori, honeysuckle, platycodon grandiflorum, poria cocos, donkey-hide gelatin, folium mori, radix puerariae, honey, ground coffee and non-dairy creamer. The preparation method comprises the steps of washing all the raw materials except the honey, the ground coffee and the non-dairy creamer, draining off and then grinding the raw materials into a thick liquid; feeding ethyl alcohol into the obtained thick liquid, carrying out ultrasonic extraction twice, carrying out suction filtration on the product, mixing the filtrates, heating and concentrating to obtain the extract; and feeding the honey, the ground coffee and the non-dairy creamer into the obtained extract, evenly mixing, then concentrating, drying, pulverizing, and granulating to obtain the traditional Chinese medicine coffee with functions of improving immunity and relieving fatigue. The traditional Chinese medicines of the coffee drink derive from 84 medicines with homology of medicine and food issued by the ministry of health, so that the coffee has the characteristics of being multiple in functions and wide in appropriate crowd; and the traditional Chinese medicine coffee has a good effect especially for improving the immunity of a human body and relieving fatigue.