5 results about "Bacterial enzymes" patented technology

This invention relates to the field of low-temperature fermentation formulation technology. The invention provides a low-temperature resistant compound bacterial enzyme formulation, its preparation method, and its application in the co-fermentation of straw and manure. The compound bacterial enzyme formulation comprises the following raw materials in parts by weight: 8-10 parts of compound bacterial agent, 1-1.4 parts of compound enzyme agent, and 88.6-91 parts of excipients. The compound bacterial enzyme formulation of this invention can ferment straw and manure under conventional conditions as well as at low temperatures of around 15°C, effectively meeting the fermentation requirements in low-temperature regions. By combining the excipients with the bacteria and enzymes, substances and nutrients during the fermentation process can be effectively adsorbed, avoiding acid inhibition, and providing attachment sites for the bacteria and enzymes, thus prolonging their activity.

This invention belongs to the field of component detection technology and discloses a method and application for the detection of octadecyl vinyl ether. Using ginseng prepared by co-fermentation of ginseng powder and bacterial enzymes as the sample, a three-step pretreatment method is employed: ethanol extraction combined with liquid-liquid extraction of a toluene:n-hexane mixture (5:1 volume ratio) and PDMS solid-phase microextraction. This is combined with gas chromatography-tandem mass spectrometry (GC-MS) to achieve qualitative and quantitative analysis of octadecyl vinyl ether. The three-step pretreatment method uses ethanol extraction combined with liquid-liquid extraction of a toluene:n-hexane mixture and PDMS solid-phase microextraction. The ethanol extractant is consistent with the solvent used in the preparation of fermented ginseng, ensuring sufficient dissolution of the target analyte. The toluene-n-hexane mixed solvent efficiently separates the target analyte from polar matrix impurities, and the boiling point difference facilitates subsequent solvent removal. PDMS solid-phase microextraction further enriches trace amounts of the target analyte. Compared with the reduced pressure concentration method, the detection sensitivity is improved by more than 2 times, and matrix interference is removed more thoroughly.

The application discloses a kind of low-pressure steam explosion coupling bacterial enzyme synergistic fermentation of rape straw feed and its preparation method, it is related to agricultural waste resource utilization and feed processing technical field, including the following steps: after cutting, natural sun-dried rape straw is loaded into steam explosion equipment, under the condition of setting, steam explosion is carried out, then instantaneously decompression is obtained Steam explosion rape straw, collect steam explosion liquid, and wait for it to restore normal temperature for standby;Compound bacterial enzyme preparation is dissolved in the collected normal temperature steam explosion liquid, is fully stirred, and forms uniform bacterial enzyme-steam explosion liquid mixed preparation;Bacterial enzyme-steam explosion liquid mixed preparation is evenly sprayed to the surface of steam explosion rape straw, and it is ensured that mixed preparation and straw are fully contacted, evenly distributed while spraying while turning over;After mixing, the material is rapidly loaded into fermentation bag, compacts and seals, fermentation, and is obtained immediately.The application can effectively reduce the crude fiber and glucosinolate content of rape straw, reduce the loss of nutritional ingredients, improve the nutritional value, and promote the low-cost feed utilization of rape straw.

The present application belongs to the field of microbial technology, and particularly relates to a micro-ecological preparation, a composite preparation thereof and application. The present application discloses a micro-ecological preparation and a composite preparation thereof. The composite preparation comprises the micro-ecological preparation and a composite enzyme preparation. The micro-ecological composite preparation comprises Lactobacillus plantarum CGMCC NO.12849, Bacillus subtilis CGMCC NO.25499 and Saccharomyces cerevisiae CGMCC NO.21678. The composite enzyme preparation comprises cellulase, xylanase and pectinase respectively. The endotoxin-degrading bacterial enzyme composite preparation realizes that the endotoxin degradation rate of feed reaches more than 95%, and solves the problems of animal health and production loss caused by feed endotoxin pollution.

Provided herein is the first commensal microbial gene encoding an enzyme that catalyzes the conversion of androstenedione to epitestosterone in the gut bacterium Clostridium scindens (desF). Also provided is an important and unrecognized capability for epitestosterone in androgen receptor-dependent prostate cancer cell proliferation and the potential clinical relevance of this bacterial enzymatic pathway (desF) in prostate cancer. Additionally, it was discovered that bacterial isolates from urine or prostatectomy tissue are capable of androgen production and that urinary tract bacteria can exhibit 17β-hydroxysteroid dehydrogenase activity The gene in urinary isolates encoding 17β-hydroxysteroid dehydrogenase that catalyzes the conversion of androstenedione to testosterone was identified and named desG.