14 results about "Bacterial enzymes" patented technology

This invention discloses a quantitative detection kit for arginine peptidase, a qualitative detection kit for arginine peptidase, and their usage methods. The quantitative detection kit utilizes the specific substrate Nα-benzoyl-DL-arginine-4-ylbenzamide hydrochloride, which can be hydrolyzed by arginine peptidase to 4-ylbenzamide. 4-ylbenzamide has a maximum absorption peak at 405 nm, and the specific arginine peptidase activity can be rapidly detected at room temperature using spectrophotometry. The qualitative kit utilizes the specific substrate Nα-benzoyl-DL-arginine-β-naphthamide hydrochloride, which can be hydrolyzed by arginine peptidase to p-β-naphthamide. β-naphthamide combines with 4-(dimethylamino)cinnamaldehyde to form a compound that is visible to the naked eye as a pink color. The disulfide bond cleaving agent cuts the mucus disulfide bonds, exposing bacterial enzyme sites, and the synergistic effect of the enzyme activator enhances the reaction rate of arginine peptidase hydrolysis of the substrate, significantly improving the detection sensitivity of arginine peptidase in tongue coating samples.

This invention relates to the field of low-temperature fermentation formulation technology. The invention provides a low-temperature resistant compound bacterial enzyme formulation, its preparation method, and its application in the co-fermentation of straw and manure. The compound bacterial enzyme formulation comprises the following raw materials in parts by weight: 8-10 parts of compound bacterial agent, 1-1.4 parts of compound enzyme agent, and 88.6-91 parts of excipients. The compound bacterial enzyme formulation of this invention can ferment straw and manure under conventional conditions as well as at low temperatures of around 15°C, effectively meeting the fermentation requirements in low-temperature regions. By combining the excipients with the bacteria and enzymes, substances and nutrients during the fermentation process can be effectively adsorbed, avoiding acid inhibition, and providing attachment sites for the bacteria and enzymes, thus prolonging their activity.

The present invention relates to an antimicrobial composite comprising a cationic antimicrobial compound and a bacterial enzyme degradable anionic copolymer wherein the bacterial enzyme degradable anionic copolymer and the cationic antimicrobial compound are linked to each other by electrostatic interaction. The invention also relates to a pharmaceutical composition comprising the antibacterial composite; a method of treating a subject suffering from a bacterial and / or fungal infection, comprising the step of administering to the subject a therapeutically effective amount of an antibacterial composite or pharmaceutical composition; the use of an antimicrobial composite in the preparation of a medicament for the treatment of bacterial and / or fungal infections in a subject in need of an antimicrobial composite; the use of the antibacterial composite or pharmaceutical composition for the treatment of bacterial and / or fungal infections; the invention also provides a method for preparing the antibacterial composite material.

The invention discloses a composite flora and activated mineral synergistic bio-organic fertilizer and a preparation method thereof. The preparation method comprises the following steps: crushing and mixing organic raw materials, adding a compound bacterium enzyme preparation, and sequentially carrying out two-stage temperature-controlled fermentation of high-temperature aerobic fermentation and medium-temperature curing to obtain a high-activity fermented material; the preparation method comprises the following steps: carrying out nano micropore activation treatment on monoclinoptilolite by using a hydrochloric acid solution to obtain activated zeolite with high specific surface area; and finally, mixing the fermented material with the activated zeolite in proportion, chelating at low temperature, granulating and drying to obtain the bio-organic fertilizer. Through composite flora construction, mineral nano activation and a step-by-step synergistic process, the effective viable count of the product reaches 2.0 billion CFU / g or above, the Pb < 2 + > adsorption rate exceeds 85%, and nutrient slow release and remarkable growth promoting effects are achieved. The product integrates the functions of soil improvement, disease inhibition, heavy metal restoration and nutrient slow release, and is suitable for ecological planting of various crops.

The invention discloses a bacterium-enzyme synergistic microbial preparation and a preparation method and application thereof, and the bacterium-enzyme synergistic microbial preparation comprises a mixed bacterium suspension containing bacillus velezensis and bacillus altitudinis; the thallus concentration ratio of the bacillus velezensis to the bacillus altitudinis is (3: 1)-(1: 3); the mixed bacterial suspension also contains cellulase with the mass fraction of 0.5%-3%. After bacteria-enzyme synergistic fermentation treatment, the coordination of conventional chemical components of the tobacco leaves is improved. Moreover, after the bacteria-enzyme synergistic fermentation treatment, the volatile aroma components of the tobacco leaves are remarkably increased, and the content of the volatile aroma components such as megastigmatrienone, benzyl alcohol, phenethyl alcohol and damascenone is remarkably increased.

The invention discloses a compound bacterium enzyme preparation for efficiently degrading shrub lignocellulose and application of the compound bacterium enzyme preparation, and relates to the technical field of agricultural microorganisms. The preparation consists of efficient lignocellulose degrading bacteria obtained by screening and a compound enzyme system (cellulase, xylanase and laccase in a ratio of 5: 3: 2) in a specific ratio, and is prepared by an optimized fermentation process. The viable count of the product is greater than or equal to 1 * 10 < 8 > CFU / g, neutral detergent fibers and acid detergent fibers of shrubs can be respectively reduced by 6.4% and 8.27%, the in-vitro dry matter digestibility is improved by 13%, and the product can be stably preserved for more than 6 months at room temperature. The preparation method comprises fermentation liquor preparation and shrub raw material treatment, is simple and convenient to use, can significantly improve the nutritional value of the shrub feed, reduces the breeding cost, and provides an economic and effective solution for the feed utilization of shrub resources in desertification regions.

This invention belongs to the field of component detection technology and discloses a method and application for the detection of octadecyl vinyl ether. Using ginseng prepared by co-fermentation of ginseng powder and bacterial enzymes as the sample, a three-step pretreatment method is employed: ethanol extraction combined with liquid-liquid extraction of a toluene:n-hexane mixture (5:1 volume ratio) and PDMS solid-phase microextraction. This is combined with gas chromatography-tandem mass spectrometry (GC-MS) to achieve qualitative and quantitative analysis of octadecyl vinyl ether. The three-step pretreatment method uses ethanol extraction combined with liquid-liquid extraction of a toluene:n-hexane mixture and PDMS solid-phase microextraction. The ethanol extractant is consistent with the solvent used in the preparation of fermented ginseng, ensuring sufficient dissolution of the target analyte. The toluene-n-hexane mixed solvent efficiently separates the target analyte from polar matrix impurities, and the boiling point difference facilitates subsequent solvent removal. PDMS solid-phase microextraction further enriches trace amounts of the target analyte. Compared with the reduced pressure concentration method, the detection sensitivity is improved by more than 2 times, and matrix interference is removed more thoroughly.

The application discloses a kind of low-pressure steam explosion coupling bacterial enzyme synergistic fermentation of rape straw feed and its preparation method, it is related to agricultural waste resource utilization and feed processing technical field, including the following steps: after cutting, natural sun-dried rape straw is loaded into steam explosion equipment, under the condition of setting, steam explosion is carried out, then instantaneously decompression is obtained Steam explosion rape straw, collect steam explosion liquid, and wait for it to restore normal temperature for standby;Compound bacterial enzyme preparation is dissolved in the collected normal temperature steam explosion liquid, is fully stirred, and forms uniform bacterial enzyme-steam explosion liquid mixed preparation;Bacterial enzyme-steam explosion liquid mixed preparation is evenly sprayed to the surface of steam explosion rape straw, and it is ensured that mixed preparation and straw are fully contacted, evenly distributed while spraying while turning over;After mixing, the material is rapidly loaded into fermentation bag, compacts and seals, fermentation, and is obtained immediately.The application can effectively reduce the crude fiber and glucosinolate content of rape straw, reduce the loss of nutritional ingredients, improve the nutritional value, and promote the low-cost feed utilization of rape straw.

The application discloses a traditional Chinese medicine bacteria enzyme preparation for promoting intestinal health and stress resistance of piglets, and belongs to the technical field of feed additives. The traditional Chinese medicine bacteria enzyme preparation comprises the following components: compound traditional Chinese medicinal materials, i.e., eucommia ulmoides, astragalus membranaceus, anise, wild chrysanthemum and platycodon grandiflorum; composite enzymes, i.e., cellulase, xylanase and glutamine transaminase; probiotics, i.e., corynebacterium glutamicum, fusing weissella and bacteroide fragilis; base material, i.e., corn flour and soybean meal; intestinal nutrient, i.e., glutamine; and anti-stress preparation, i.e., vitamin C and tryptophan. The compound traditional Chinese medicinal materials, the cellulase, the xylanase and the glutamine transaminase, and the corynebacterium glutamicum are subjected to aerobic fermentation, then the fusing weissella and the bacteroide fragilis are added to perform anaerobic fermentation, finally, the intestinal nutrient and the anti-stress preparation are supplemented, so that the traditional Chinese medicine bacteria enzyme preparation capable of significantly promoting intestinal health and improving stress resistance of weaned piglets is obtained, and an important foundation is laid for health management and efficient production of weaned piglets.

The invention relates to the technical field of preparation of food flavor agents, in particular to a butter type bacterium-enzyme co-promoted cheese flavor agent and a processing technology. The flavor agent provided by the invention is composed of hydrolase and active lactic acid bacteria, the hydrolase is prepared from Lipozyme TLIM C lipase, the active lactic acid bacteria are prepared from lactococcus lactis BL-19, the fermentation substrate is butter, and the natural butter is subjected to aroma enhancement treatment to prepare the milk flavor base, so that the cost is reduced, the dosage is reduced, and the product grade is improved at relatively low cost.

The invention relates to a bacterium enzyme composite deodorant and application thereof, which can effectively improve the digestive function of the gastrointestinal tract of a piglet to promote the growth of the piglet, obviously reduce the emission of volatile malodorous substances in excrement, and provide a new feed additive for healthy breeding of the piglet and improvement of the breeding environment. Wherein the compound bacteria are prepared by uniformly mixing lactobacillus plantarum, saccharomyces cerevisiae and bacillus licheniformis according to the weight ratio of 3: 2: 1, the compound enzyme is prepared by uniformly mixing protease and amylase according to the weight ratio of 1: 25, and the compound bacteria and the compound enzyme are uniformly mixed according to the weight ratio of 2: 1. As a feed additive, the feed additive can significantly improve the average daily gain of piglets, improve the digestive function of intestinal tracts of the piglets, improve the growth performance of the piglets, reduce the feed-gain ratio, effectively reduce the pH value and the water content of excrement and reduce emission of volatile malodorous substances such as ammonia gas, hydrogen sulfide and methyl mercaptan, and is an efficient, green, ecological and safe feed additive. The method has popularization and application value in various pig farms.

The present application belongs to the field of microbial technology, and particularly relates to a micro-ecological preparation, a composite preparation thereof and application. The present application discloses a micro-ecological preparation and a composite preparation thereof. The composite preparation comprises the micro-ecological preparation and a composite enzyme preparation. The micro-ecological composite preparation comprises Lactobacillus plantarum CGMCC NO.12849, Bacillus subtilis CGMCC NO.25499 and Saccharomyces cerevisiae CGMCC NO.21678. The composite enzyme preparation comprises cellulase, xylanase and pectinase respectively. The endotoxin-degrading bacterial enzyme composite preparation realizes that the endotoxin degradation rate of feed reaches more than 95%, and solves the problems of animal health and production loss caused by feed endotoxin pollution.

The invention provides a seedling raising substrate and a preparation method and application thereof. The seedling raising substrate is prepared from the following raw materials in parts by weight: 30-50 parts of traditional Chinese medicine residues; 20 to 40 parts of gramineous crop residues; 15 to 30 parts of sulfhydrylation modified sugar beet pulp; 2-6 parts of a hydrolase preparation; 1-4 parts of a fermentation inoculant; wherein the sulfhydrylation modified sugar beet pulp is prepared by reacting sugar beet pulp with a sulfhydryl-containing compound. According to the seedling culture substrate, the traditional Chinese medicine residues, the gramineous crop residues and the sulfhydrylation modified sugar beet pulp serve as core raw materials, and efficient resource utilization of waste is achieved; the sulfhydrylation modified sugar beet pulp can effectively chelate heavy metal ions in the substrate, so that the environment-friendly safety and the use reliability of the seedling substrate are improved; the hydrolase preparation and the fermentation inoculant jointly form a bacterium-enzyme synergistic system, so that the mineralization and humification efficiency of organic matters is remarkably improved, the nutrient conversion efficiency and the bioavailability are improved, and continuous and balanced nutrition supply is provided for seedlings.

Provided herein is the first commensal microbial gene encoding an enzyme that catalyzes the conversion of androstenedione to epitestosterone in the gut bacterium Clostridium scindens (desF). Also provided is an important and unrecognized capability for epitestosterone in androgen receptor-dependent prostate cancer cell proliferation and the potential clinical relevance of this bacterial enzymatic pathway (desF) in prostate cancer. Additionally, it was discovered that bacterial isolates from urine or prostatectomy tissue are capable of androgen production and that urinary tract bacteria can exhibit 17β-hydroxysteroid dehydrogenase activity The gene in urinary isolates encoding 17β-hydroxysteroid dehydrogenase that catalyzes the conversion of androstenedione to testosterone was identified and named desG.