38 results about "Bacterial enzymes" patented technology

The invention discloses a bacterium-enzyme mixed preparation for PET plastic degradation and a preparation method thereof, and belongs to the technical field of enzyme preparations, a lanthanum metal framework is formed, loaded on the surface of titanium dioxide and carbonized to obtain a lanthanum-doped porous carbon layer, and then nitric acid treatment and silane coupling agent treatment are performed to significantly increase the specific surface area and roughness, so that the specific surface area is increased; the calcium-based diatomite is taken as a carrier to form a sodium alginate microcapsule taking an enzyme preparation as an inclusion, and the sodium alginate microcapsule is loaded on the surface of the calcium-based diatomite, so that the situation that titanium dioxide generates hydroxyl free radicals in photocatalysis to directly attack the enzyme preparation is avoided, and the degradation effect on the PET plastic is prolonged; calcium ions in the calcium-based diatomite can increase the crosslinking degree with the sodium alginate, the load rate of the sodium alginate microcapsule is increased, the strength of the microcapsule is improved, and the slow release effect of an enzyme preparation in the microcapsule is further improved.

The invention discloses a method for improving the quality of frozen dough by using lactic acid bacteria and saccharomycetes synergistic enzyme treatment technology, which comprises the following steps: (1) carrying out primary culture on lactic acid bacteria and saccharomycetes, and then carrying out inoculated culture again to obtain a bacterial suspension; (2) centrifuging the bacterial suspension to obtain bacterial sludge; (3) preparing dough: adding enzyme into a proper amount of wheat flour, water, salt, sugar and the like, inoculating lactic acid bacteria and saccharomycetes, and uniformly mixing; (4) uniformly stirring the flour batting, shaping, and carrying out primary fermentation; (5) after the preliminary fermentation is completed, quickly freezing at the temperature of-30 DEG C to-40 DEG C until the central temperature of the dough is reduced to-18 DEG C; (6) freezing and storing the frozen dough at-18 DEG C; according to the invention, the water loss of the frozen dough in the freezing storage process is improved, the problem of freeze drying loss is solved, the fermentation performance of the frozen dough is remarkably improved, the hardness of the frozen steamed buns is effectively reduced, the elasticity, specific volume and water holding rate are improved, and the quality and processing performance of the frozen dough are improved to a great extent.

This invention discloses a quantitative detection kit for arginine peptidase, a qualitative detection kit for arginine peptidase, and their usage methods. The quantitative detection kit utilizes the specific substrate Nα-benzoyl-DL-arginine-4-ylbenzamide hydrochloride, which can be hydrolyzed by arginine peptidase to 4-ylbenzamide. 4-ylbenzamide has a maximum absorption peak at 405 nm, and the specific arginine peptidase activity can be rapidly detected at room temperature using spectrophotometry. The qualitative kit utilizes the specific substrate Nα-benzoyl-DL-arginine-β-naphthamide hydrochloride, which can be hydrolyzed by arginine peptidase to p-β-naphthamide. β-naphthamide combines with 4-(dimethylamino)cinnamaldehyde to form a compound that is visible to the naked eye as a pink color. The disulfide bond cleaving agent cuts the mucus disulfide bonds, exposing bacterial enzyme sites, and the synergistic effect of the enzyme activator enhances the reaction rate of arginine peptidase hydrolysis of the substrate, significantly improving the detection sensitivity of arginine peptidase in tongue coating samples.

This invention relates to the field of low-temperature fermentation formulation technology. The invention provides a low-temperature resistant compound bacterial enzyme formulation, its preparation method, and its application in the co-fermentation of straw and manure. The compound bacterial enzyme formulation comprises the following raw materials in parts by weight: 8-10 parts of compound bacterial agent, 1-1.4 parts of compound enzyme agent, and 88.6-91 parts of excipients. The compound bacterial enzyme formulation of this invention can ferment straw and manure under conventional conditions as well as at low temperatures of around 15°C, effectively meeting the fermentation requirements in low-temperature regions. By combining the excipients with the bacteria and enzymes, substances and nutrients during the fermentation process can be effectively adsorbed, avoiding acid inhibition, and providing attachment sites for the bacteria and enzymes, thus prolonging their activity.

The present invention relates to an antimicrobial composite comprising a cationic antimicrobial compound and a bacterial enzyme degradable anionic copolymer wherein the bacterial enzyme degradable anionic copolymer and the cationic antimicrobial compound are linked to each other by electrostatic interaction. The invention also relates to a pharmaceutical composition comprising the antibacterial composite; a method of treating a subject suffering from a bacterial and / or fungal infection, comprising the step of administering to the subject a therapeutically effective amount of an antibacterial composite or pharmaceutical composition; the use of an antimicrobial composite in the preparation of a medicament for the treatment of bacterial and / or fungal infections in a subject in need of an antimicrobial composite; the use of the antibacterial composite or pharmaceutical composition for the treatment of bacterial and / or fungal infections; the invention also provides a method for preparing the antibacterial composite material.

The invention discloses a composite flora and activated mineral synergistic bio-organic fertilizer and a preparation method thereof. The preparation method comprises the following steps: crushing and mixing organic raw materials, adding a compound bacterium enzyme preparation, and sequentially carrying out two-stage temperature-controlled fermentation of high-temperature aerobic fermentation and medium-temperature curing to obtain a high-activity fermented material; the preparation method comprises the following steps: carrying out nano micropore activation treatment on monoclinoptilolite by using a hydrochloric acid solution to obtain activated zeolite with high specific surface area; and finally, mixing the fermented material with the activated zeolite in proportion, chelating at low temperature, granulating and drying to obtain the bio-organic fertilizer. Through composite flora construction, mineral nano activation and a step-by-step synergistic process, the effective viable count of the product reaches 2.0 billion CFU / g or above, the Pb < 2 + > adsorption rate exceeds 85%, and nutrient slow release and remarkable growth promoting effects are achieved. The product integrates the functions of soil improvement, disease inhibition, heavy metal restoration and nutrient slow release, and is suitable for ecological planting of various crops.

The invention discloses a bacterium-enzyme synergistic microbial preparation and a preparation method and application thereof, and the bacterium-enzyme synergistic microbial preparation comprises a mixed bacterium suspension containing bacillus velezensis and bacillus altitudinis; the thallus concentration ratio of the bacillus velezensis to the bacillus altitudinis is (3: 1)-(1: 3); the mixed bacterial suspension also contains cellulase with the mass fraction of 0.5%-3%. After bacteria-enzyme synergistic fermentation treatment, the coordination of conventional chemical components of the tobacco leaves is improved. Moreover, after the bacteria-enzyme synergistic fermentation treatment, the volatile aroma components of the tobacco leaves are remarkably increased, and the content of the volatile aroma components such as megastigmatrienone, benzyl alcohol, phenethyl alcohol and damascenone is remarkably increased.

The invention discloses a multi-bacteria enzyme composition for water purification treatment and application, and relates to the technical field of water purification. The microbial agent is prepared from the following components in parts by weight: 15 to 30 parts of rhodopseudomonas palustris, 15 to 30 parts of bacillus subtilis, 25 to 35 parts of serratia marcescens, 25 to 35 parts of denitrifying pseudomonas, 25 to 35 parts of lactic acid coccus, 25 to 35 parts of bacillus amyloliquefaciens and 25 to 35 parts of rhodotorula benthica. The synergistic effect of the flora can inhibit the outbreak of harmful algae, promote the growth of beneficial algae, improve the flocculation performance of a water body and effectively improve the transparency. Meanwhile, the composition has a remarkable removal effect on key pollution indexes such as ammonia nitrogen, COD (Chemical Oxygen Demand), BOD (Biochemical Oxygen Demand), suspended solids and total phosphorus, so that the systematic repair of the water body is realized.

The invention relates to fermented soft granules for livestock and poultry and a preparation method thereof, and belongs to the technical field of livestock and poultry feed. The granular feed comprises the following components: 30-60% of starch, 10-30% of iced fresh fish, 10-30% of protein, 0.5-1% of vitamins, 0.5-3% of minerals, 2-5% of fat and 0.5-2% of compound bacteria enzyme. The granular material achieves the purposes of reducing the breeding cost, increasing the absorption and utilization rate, improving the immunity of bred animals and being free of resistance, promotes healthy breeding, guarantees food safety, and also can improve the eating taste and flavor of the bred animals and the food quality. The problems that an existing feed is low in production ratio, and the yield is not easy to increase are solved.

The invention belongs to the technical field of bacterium-enzyme synergistic fermentation methods, and particularly relates to a bacterium-enzyme synergistic fermentation method with high fermentation quality, which comprises the following steps: taking fresh moringa oleifera and morinda citrifolia stems and leaves, crushing the moringa oleifera and morinda citrifolia stems and leaves by a cutting machine until the particle size is 1-2cm, drying the crushed moringa oleifera and morinda citrifolia stems and leaves in the shade for 6 hours, and controlling the moisture content of the materials dried in the shade to be 65-75%, an enzyme microbial agent is added to the material dried in the shade and mixed to be uniform, the enzyme microbial agent is composed of lactic acid bacteria, bacillus licheniformis, aspergillus niger, cellulase, phytase and xylanase, and the dosage of the components is that the lactic acid bacteria accounts for 0.15% of the total mass of the material dried in the shade, the bacillus licheniformis accounts for 0.15% of the total mass of the material dried in the shade, the aspergillus niger accounts for 0.15% of the total mass of the material dried in the shade, the cellulase accounts for 0.1% of the total mass of the material dried in the shade, the phytase accounts for 0.1% of the total mass of the material dried in the shade, and the xylanase accounts for 0.1% of the total mass of the material dried in the shade. The materials mixed with the enzyme microbial inoculum are loaded into polyethylene silage fermentation bags, vacuum packaging and sealing are conducted, the sealed polyethylene silage fermentation bags are placed in a shady and cool place, and fermentation is conducted for 28 days.

The invention relates to the technical field of flavor agents, in particular to a cream type bacterium-enzyme co-promoted cheese flavor agent with good sensory characteristics and a preparation method of the cream type bacterium-enzyme co-promoted cheese flavor agent. The flavor agent provided by the invention is composed of hydrolase and active lactic acid bacteria, the hydrolase is prepared from Protease AY 'Amano' 400SD protease, the active lactic acid bacteria are prepared from lactococcus lactis BL-19 (preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC 16242), and the fermentation substrate is cream.

The invention provides a special Chinese herbal medicine and attapulgite compound fermented feed for cattle in a fattening period as well as a preparation method and application thereof, and belongs to the technical field of feed processing. The Chinese herbal medicine and attapulgite compound fermented feed is prepared from the following raw materials: radix astragali leftover materials, radix bupleuri leftover materials, silverweed cinquefoil root leftover materials, paper mulberry leaves, liquorice leftover materials, fennel leftover materials, corncobs, mixed-vitamin attapulgite clay and a bacterium enzyme mixture. The Chinese herbal medicines and the attapulgite clay are subjected to mixed fermentation by utilizing various microorganisms and enzymes, so that the digestion and utilization rate of nutrient substances of the raw materials of the feed can be increased, anti-nutritional factors can be passivated, the production performance and meat quality of meat / yaks can be effectively improved, the immunity of the meat / yaks can be improved, diseases can be reduced, and the feeding cost can be reduced; the use amount of antibiotics is reduced, and the problems of antibiotic residues and environmental pollution are reduced.

The invention relates to the technical field of biological fermentation, in particular to a method for synergistically fermenting distillers' grains by bacteria and enzymes. The viable count of the three functional bacteria provided by the invention can reach 107CFU / g or above after the three functional bacteria are cultured for 5 days in an environment with the temperature of 50-60 DEG C and the pH value of 3.0-4.0. In the environment, indigenous flora naturally carried by the yellow wine lees are difficult to survive, interference of indigenous bacteria can be basically eliminated in the fermentation process, growth and reproduction of functional bacteria are facilitated, and the stability of large-scale fermentation can be improved. The bacterial enzyme synergistic process activates the metabolic diversity of microorganisms through enzymolysis, the content of propionic acid and acetic acid is remarkably increased, meanwhile, succinic acid is kept stable, and the antibacterial activity and metabolic balance of an organic acid spectrum can be optimized. Meanwhile, enzymolysis can promote degradation of medium-chain fatty acid, and enzymolysis continuously optimizes a lipid metabolism structure in the later stage. The proline content of a bacterium-enzyme synergistic fermentation group is far higher than that of a pure fermentation group, so that the palatability of the feed is remarkably improved. Moreover, methionine is kept stable in a bacterium-enzyme synergistic group, so that nutrition loss can be avoided.

The present application relates to a kind of high conversion rate degradation cotton stalk free gossypol enzyme combination, in view of the existing cotton stalk free gossypol strong toxicity, low degradation efficiency, lead to its resource utilization is limited, the present application obtains efficient degradation strain and compound specific enzyme preparation, constructs and forms bacteria enzyme combination system.The bacteria enzyme combination includes degradation strain fermentation liquor and composite enzyme preparation, wherein the degradation strain is the mixed strain of pseudomonas and bacillus, and the composite enzyme preparation is compounded by laccase, peroxidase and esterase according to a specific ratio.The degradation rate of the combination to free gossypol in cotton stalk can reach more than 95%, which is significantly better than the degradation effect of single strain or single enzyme preparation.

The invention discloses a compound bacterium enzyme preparation for efficiently degrading shrub lignocellulose and application of the compound bacterium enzyme preparation, and relates to the technical field of agricultural microorganisms. The preparation consists of efficient lignocellulose degrading bacteria obtained by screening and a compound enzyme system (cellulase, xylanase and laccase in a ratio of 5: 3: 2) in a specific ratio, and is prepared by an optimized fermentation process. The viable count of the product is greater than or equal to 1 * 10 < 8 > CFU / g, neutral detergent fibers and acid detergent fibers of shrubs can be respectively reduced by 6.4% and 8.27%, the in-vitro dry matter digestibility is improved by 13%, and the product can be stably preserved for more than 6 months at room temperature. The preparation method comprises fermentation liquor preparation and shrub raw material treatment, is simple and convenient to use, can significantly improve the nutritional value of the shrub feed, reduces the breeding cost, and provides an economic and effective solution for the feed utilization of shrub resources in desertification regions.

This invention belongs to the field of component detection technology and discloses a method and application for the detection of octadecyl vinyl ether. Using ginseng prepared by co-fermentation of ginseng powder and bacterial enzymes as the sample, a three-step pretreatment method is employed: ethanol extraction combined with liquid-liquid extraction of a toluene:n-hexane mixture (5:1 volume ratio) and PDMS solid-phase microextraction. This is combined with gas chromatography-tandem mass spectrometry (GC-MS) to achieve qualitative and quantitative analysis of octadecyl vinyl ether. The three-step pretreatment method uses ethanol extraction combined with liquid-liquid extraction of a toluene:n-hexane mixture and PDMS solid-phase microextraction. The ethanol extractant is consistent with the solvent used in the preparation of fermented ginseng, ensuring sufficient dissolution of the target analyte. The toluene-n-hexane mixed solvent efficiently separates the target analyte from polar matrix impurities, and the boiling point difference facilitates subsequent solvent removal. PDMS solid-phase microextraction further enriches trace amounts of the target analyte. Compared with the reduced pressure concentration method, the detection sensitivity is improved by more than 2 times, and matrix interference is removed more thoroughly.

The invention belongs to the crossing field of nanotechnology, synthetic biology and sewage treatment, and particularly relates to a hydroxylamine oxidation-reduction nano-enzyme, a bacterium-enzyme compound and application of the hydroxylamine oxidation-reduction nano-enzyme and the bacterium-enzyme compound in nitrite synthesis. The hydroxylamine redox nano-enzyme (Ir-coated PVP nano-enzyme) is obtained through a solvothermal method reaction of an iridium salt precursor and polyvinylpyrrolidone in an organic solvent, the molar ratio of the iridium salt precursor to the polyvinylpyrrolidone is (10-100): 1, and the Ir-coated PVP nano-enzyme can catalyze substrate hydroxylamine to be oxidized into nitrite. The engineering strain with the novel ammoxidation capacity is obtained through modification, ammonia nitrogen in culture or sewage can be converted into hydroxylamine, and a catalytic substrate is provided for nano-enzyme. The nano-enzyme is high in catalytic efficiency and stable in activity, and the use cost can be saved. Therefore, the Ir-coated PVP nano-enzyme and bacterial enzyme compound constructed by the invention has huge social benefits, economic benefits and good industrial application prospects.

The invention provides an antimicrobial composite material, comprising a bacterial-enzyme- degradable anionic copolymer, and a plurality of metal ions, wherein the bacterial-enzyme- degradable anionic copolymer and the plurality of metal ions form a complex. The invention also provides a pharmaceutical composition and a composition comprising the antimicrobial composite material. In addition, the invention provides the antimicrobial composite material and the pharmaceutical composition for use as a medicament.

The invention provides a solid-state bacterium-enzyme synergistic fermented feed as well as a preparation method and application thereof. By establishing and optimizing a synergistic fermentation process of complex microbial inoculants such as bacillus subtilis, lactobacillus plantarum, saccharomyces cerevisiae, aspergillus niger and the like and nine types of enzyme preparations, it is found that the fermentation can significantly improve the content of nutritional ingredients of the feed and effectively degrade toxins and anti-nutritional factors. 16S rRNA gene sequencing and flavor omics sequencing find that the fermentation can also significantly improve the abundance of microorganisms such as lactobacillus and pediococcus in the feed, and produced flavor substances endow the feed with outstanding sweet, fruity, green grass and waxy taste characteristics. In addition, through further observation of feeding growing pigs, the feed can significantly improve the growth performance of the pigs, enhance the oxidation resistance of the body, and effectively improve the small intestine tissue morphology and the intestinal barrier function. The feed effectively solves the problems of slow growth and high incidence of intestinal diseases of young pigs, and provides key technical support for antibiotic-free breeding.

The application discloses a kind of low-pressure steam explosion coupling bacterial enzyme synergistic fermentation of rape straw feed and its preparation method, it is related to agricultural waste resource utilization and feed processing technical field, including the following steps: after cutting, natural sun-dried rape straw is loaded into steam explosion equipment, under the condition of setting, steam explosion is carried out, then instantaneously decompression is obtained Steam explosion rape straw, collect steam explosion liquid, and wait for it to restore normal temperature for standby;Compound bacterial enzyme preparation is dissolved in the collected normal temperature steam explosion liquid, is fully stirred, and forms uniform bacterial enzyme-steam explosion liquid mixed preparation;Bacterial enzyme-steam explosion liquid mixed preparation is evenly sprayed to the surface of steam explosion rape straw, and it is ensured that mixed preparation and straw are fully contacted, evenly distributed while spraying while turning over;After mixing, the material is rapidly loaded into fermentation bag, compacts and seals, fermentation, and is obtained immediately.The application can effectively reduce the crude fiber and glucosinolate content of rape straw, reduce the loss of nutritional ingredients, improve the nutritional value, and promote the low-cost feed utilization of rape straw.

The invention relates to the technical field of pharmaceutical preparations, and discloses a degradable intestinal drug targeted release capsule and a preparation method thereof, the method comprises the following steps: preparation of an enteric shell layer, construction of an isolation layer, preparation of a drug core, and assembly and sealing of the capsule; by designing a multi-layer structure composed of an enteric shell layer, a pH / enzyme dual-response isolation layer and a drug loading core layer, targeted release of a drug at a colon part is realized; the enteric shell layer can resist a gastric acid environment, so that the medicine can safely pass through the stomach; after entering the intestinal tract, the shell is dissolved under a specific pH value, and the exposed isolation layer can respond to the higher pH value of the colon part and the specific flora enzyme environment to be degraded, so that the burst release of the drug at the front part of the colon is prevented, and the accuracy and reliability of targeted drug delivery are improved.

The application discloses a traditional Chinese medicine bacteria enzyme preparation for promoting intestinal health and stress resistance of piglets, and belongs to the technical field of feed additives. The traditional Chinese medicine bacteria enzyme preparation comprises the following components: compound traditional Chinese medicinal materials, i.e., eucommia ulmoides, astragalus membranaceus, anise, wild chrysanthemum and platycodon grandiflorum; composite enzymes, i.e., cellulase, xylanase and glutamine transaminase; probiotics, i.e., corynebacterium glutamicum, fusing weissella and bacteroide fragilis; base material, i.e., corn flour and soybean meal; intestinal nutrient, i.e., glutamine; and anti-stress preparation, i.e., vitamin C and tryptophan. The compound traditional Chinese medicinal materials, the cellulase, the xylanase and the glutamine transaminase, and the corynebacterium glutamicum are subjected to aerobic fermentation, then the fusing weissella and the bacteroide fragilis are added to perform anaerobic fermentation, finally, the intestinal nutrient and the anti-stress preparation are supplemented, so that the traditional Chinese medicine bacteria enzyme preparation capable of significantly promoting intestinal health and improving stress resistance of weaned piglets is obtained, and an important foundation is laid for health management and efficient production of weaned piglets.

The invention relates to the technical field of preparation of food flavor agents, in particular to a butter type bacterium-enzyme co-promoted cheese flavor agent and a processing technology. The flavor agent provided by the invention is composed of hydrolase and active lactic acid bacteria, the hydrolase is prepared from Lipozyme TLIM C lipase, the active lactic acid bacteria are prepared from lactococcus lactis BL-19, the fermentation substrate is butter, and the natural butter is subjected to aroma enhancement treatment to prepare the milk flavor base, so that the cost is reduced, the dosage is reduced, and the product grade is improved at relatively low cost.

The invention relates to a bacterium enzyme composite deodorant and application thereof, which can effectively improve the digestive function of the gastrointestinal tract of a piglet to promote the growth of the piglet, obviously reduce the emission of volatile malodorous substances in excrement, and provide a new feed additive for healthy breeding of the piglet and improvement of the breeding environment. Wherein the compound bacteria are prepared by uniformly mixing lactobacillus plantarum, saccharomyces cerevisiae and bacillus licheniformis according to the weight ratio of 3: 2: 1, the compound enzyme is prepared by uniformly mixing protease and amylase according to the weight ratio of 1: 25, and the compound bacteria and the compound enzyme are uniformly mixed according to the weight ratio of 2: 1. As a feed additive, the feed additive can significantly improve the average daily gain of piglets, improve the digestive function of intestinal tracts of the piglets, improve the growth performance of the piglets, reduce the feed-gain ratio, effectively reduce the pH value and the water content of excrement and reduce emission of volatile malodorous substances such as ammonia gas, hydrogen sulfide and methyl mercaptan, and is an efficient, green, ecological and safe feed additive. The method has popularization and application value in various pig farms.

The present application belongs to the field of microbial technology, and particularly relates to a micro-ecological preparation, a composite preparation thereof and application. The present application discloses a micro-ecological preparation and a composite preparation thereof. The composite preparation comprises the micro-ecological preparation and a composite enzyme preparation. The micro-ecological composite preparation comprises Lactobacillus plantarum CGMCC NO.12849, Bacillus subtilis CGMCC NO.25499 and Saccharomyces cerevisiae CGMCC NO.21678. The composite enzyme preparation comprises cellulase, xylanase and pectinase respectively. The endotoxin-degrading bacterial enzyme composite preparation realizes that the endotoxin degradation rate of feed reaches more than 95%, and solves the problems of animal health and production loss caused by feed endotoxin pollution.

Salmonid alphavirus (SAV) is an important pathogen affecting the aquaculture of Salmonid fish. Vaccines based on DNA plasmids expressing a SAV antigen have been described. However these are difficult to prepare at large scale and at the desired quality level. Also, they are commonly administered at relatively high amounts of plasmid per animal dose. This makes such DNA vaccines less affordable for this market. The invention discloses an improved plasmid that allows effective use as a DNA vaccine against SAV, but at a much reduced amount of DNA per dose. The plasmid contains a gene encoding a bacterial enzyme: Fab.

The invention discloses a method for preparing yoghourt-flavor egg white powder by utilizing milk-derived components to regulate bacterium-enzyme synergistic fermentation, and belongs to the technical field of food processing. According to the invention, the egg white powder with low fishy smell and yoghourt flavor is obtained by adding milk-derived components to regulate and control synergistic fermentation of bacteria and enzymes. Wherein the fermentation rate of the egg white can be remarkably increased by adding the sweet whey, and the yoghourt-flavor egg white powder compounded with the sweet whey has relatively high diacetyl and acetaldehyde contents, has a mellow yoghourt flavor, is fine and smooth in texture and high in stability, and provides a basis for increasing the additional value of the egg white and developing a novel flavor egg white powder food ingredient.

This invention relates to the field of bacterial enzyme formulation technology, specifically disclosing a bacterial enzyme synergistic formulation method based on carbon and nitrogen metabolic balance regulation, comprising the following steps: collecting organic waste samples from different sources and their corresponding degradation environment data, constructing a multi-source heterogeneous bacterial-substrate-metabolic feature database; using random forest, XGBoost, or deep neural network algorithms to train and analyze the constructed bacterial-substrate-metabolic feature database, identifying key nodes to be regulated; designing a screening strategy to reverse-screen key target enzymes and strains based on the identified "key nodes to be regulated"; constructing a "bacterial enzyme synergy" multidimensional feature experimental matrix, establishing a prediction model, predicting and solving to obtain the bacterial enzyme compounding ratio and optimizing it, which significantly reduces the overall cost of industrial application while improving degradation efficiency, and has good prospects for industrialization and promotion.

The present invention belongs to the field of microorganisms and genetic engineering technology, and specifically relates to a keratinase mutant and its application in the preparation of bio-compound fertilizer from animal waste. The present invention obtains a keratinase mutant with significantly improved enzyme activity and stability and significantly improved substrate adaptability by subjecting wild-type keratinase to directed mutation in vitro. The enzyme activity of the mutant is 7216.54 U / mL at 50°C and pH 7. When used in the degradation of animal waste, it can achieve rapid and efficient degradation of keratin in different parts such as hair, skin, hooves, and horns, greatly improving the efficiency of large-scale treatment of animal waste. By fermenting animal waste with a bacterial enzyme composite preparation containing the keratinase mutant of the present invention, a bio-compound fertilizer rich in amino acid content can be obtained. The bio-compound fertilizer can be used in crop cultivation to effectively improve the disease resistance and yield of crops.

The invention provides a seedling raising substrate and a preparation method and application thereof. The seedling raising substrate is prepared from the following raw materials in parts by weight: 30-50 parts of traditional Chinese medicine residues; 20 to 40 parts of gramineous crop residues; 15 to 30 parts of sulfhydrylation modified sugar beet pulp; 2-6 parts of a hydrolase preparation; 1-4 parts of a fermentation inoculant; wherein the sulfhydrylation modified sugar beet pulp is prepared by reacting sugar beet pulp with a sulfhydryl-containing compound. According to the seedling culture substrate, the traditional Chinese medicine residues, the gramineous crop residues and the sulfhydrylation modified sugar beet pulp serve as core raw materials, and efficient resource utilization of waste is achieved; the sulfhydrylation modified sugar beet pulp can effectively chelate heavy metal ions in the substrate, so that the environment-friendly safety and the use reliability of the seedling substrate are improved; the hydrolase preparation and the fermentation inoculant jointly form a bacterium-enzyme synergistic system, so that the mineralization and humification efficiency of organic matters is remarkably improved, the nutrient conversion efficiency and the bioavailability are improved, and continuous and balanced nutrition supply is provided for seedlings.