65 results about "Bacterial enzymes" patented technology

The invention relates to the technical field of biology, in particular to a method and application of fungus-enzyme synergistic fermentation of bagasse pith, and the method comprises the following steps: (1) adjusting the water content of the bagasse pith to 60-70%, and adjusting the pH to 4.0; (2) adding the following components according to dry matter mass: 3% of phanerochaete chrysosporium liquid with the concentration of 1.5 * 10 < 8 > CFU / ml, and uniformly mixing; 1% of sucrose; the cellulase liquid is 20000U / kg, and the cellulase liquid is prepared by dissolving crude enzyme obtained by centrifuging after trichoderma hedgehog is fermented to produce enzyme in a PBS (phosphate buffer solution); and (3) putting the mixture into a fermentation tank, and performing sealed low-oxygen fermentation for 30 days to obtain the bagasse pith feed. According to the bagasse pith feed obtained by the method, the nutritional value of the bagasse pith can be improved, the fermentation quality is improved, the digestion potential is improved, the storage time is prolonged, and high-quality feed application of the bagasse is realized.

The invention discloses a compound bacterium enzyme preparation for ensiling and application of the compound bacterium enzyme preparation, and belongs to the field of bacterium enzyme compound preparations. The bacterial enzyme preparation disclosed by the invention comprises lactobacillus plantarum, lactobacillus buchneri, cellulase and xylanase, has the effects of improving the fermentation quality of silage, promoting the improvement of the organism immunity and the anti-stress capability of ruminants and the like, and has wide market value.

The invention discloses a bacterium-enzyme mixed preparation for PET plastic degradation and a preparation method thereof, and belongs to the technical field of enzyme preparations, a lanthanum metal framework is formed, loaded on the surface of titanium dioxide and carbonized to obtain a lanthanum-doped porous carbon layer, and then nitric acid treatment and silane coupling agent treatment are performed to significantly increase the specific surface area and roughness, so that the specific surface area is increased; the calcium-based diatomite is taken as a carrier to form a sodium alginate microcapsule taking an enzyme preparation as an inclusion, and the sodium alginate microcapsule is loaded on the surface of the calcium-based diatomite, so that the situation that titanium dioxide generates hydroxyl free radicals in photocatalysis to directly attack the enzyme preparation is avoided, and the degradation effect on the PET plastic is prolonged; calcium ions in the calcium-based diatomite can increase the crosslinking degree with the sodium alginate, the load rate of the sodium alginate microcapsule is increased, the strength of the microcapsule is improved, and the slow release effect of an enzyme preparation in the microcapsule is further improved.

The invention discloses a method for improving the quality of frozen dough by using lactic acid bacteria and saccharomycetes synergistic enzyme treatment technology, which comprises the following steps: (1) carrying out primary culture on lactic acid bacteria and saccharomycetes, and then carrying out inoculated culture again to obtain a bacterial suspension; (2) centrifuging the bacterial suspension to obtain bacterial sludge; (3) preparing dough: adding enzyme into a proper amount of wheat flour, water, salt, sugar and the like, inoculating lactic acid bacteria and saccharomycetes, and uniformly mixing; (4) uniformly stirring the flour batting, shaping, and carrying out primary fermentation; (5) after the preliminary fermentation is completed, quickly freezing at the temperature of-30 DEG C to-40 DEG C until the central temperature of the dough is reduced to-18 DEG C; (6) freezing and storing the frozen dough at-18 DEG C; according to the invention, the water loss of the frozen dough in the freezing storage process is improved, the problem of freeze drying loss is solved, the fermentation performance of the frozen dough is remarkably improved, the hardness of the frozen steamed buns is effectively reduced, the elasticity, specific volume and water holding rate are improved, and the quality and processing performance of the frozen dough are improved to a great extent.

The present invention relates to the technical field of bacterial enzyme fermentation, and provides a bacterial enzyme synergistic fermentation method for removing antinutritional factors in soybean meal, the method comprises the following steps: S1, soybean meal pretreatment: soybean meal, water and an auxiliary agent are mixed and then subjected to steam explosion treatment, and the auxiliary agent comprises citric acid, a hydrogen peroxide solution and polyphenol; s2, bacterial enzyme fermentation: mixing the pretreated soybean meal with molasses and water to obtain a raw material, adding a complex microbial inoculant and a complex enzyme, uniformly stirring, and fermenting; the complex microbial inoculants comprise saccharomycetes, bacillus subtilis, lactobacillus rhamnosus and pediococcus pentosaceus; the compound enzyme comprises acid protease, pectinase, phytase, glucanase, alpha-galactosidase and keratinase; and S3, after fermentation is finished, drying the materials to obtain the fermented soybean meal. According to the fermentation method, the glycinin degradation rate can reach 79% or above, the acid soluble protein content is increased to 26% or above, the anti-nutritional factor content is remarkably reduced, and the nutritional value is greatly increased.

The invention relates to a high-performance antibacterial PP plate and a preparation method thereof, and belongs to the technical field of high polymer materials. The high-performance antibacterial PP board comprises the following components in parts by weight: 60-80 parts of PP resin, 1-4 parts of a dispersing agent, 0.5-1.5 parts of a modified antibacterial agent, 2-6 parts of a modified flame retardant and 0.1-0.5 part of an antioxidant, nano-zinc oxide in the modified antibacterial agent is excited to have strong oxidizing property under ultraviolet light, destroys cell membranes, releases zinc ions to be combined with proteins and interferes bacterial enzyme activity, quaternary ammonium salt groups destroy cell membrane structures, and the quaternary ammonium salt groups and chitosan cooperate to destroy cell wall structures and permeate into the interior to inhibit bacterial activity; boron-based components in the modified flame retardant are heated to dehydrate, carbon formation is accelerated, a glassy protective layer is formed, a Si-O-B structure is constructed, incombustible gas is released, heat and oxygen are insulated, and the modified flame retardant and phosphorus-oxygen double bonds are synergistically flame-retardant; the high-performance antibacterial PP board prepared by the invention not only has a good antibacterial effect, but also has an excellent flame-retardant effect.

The invention relates to the technical field of livestock breeding, and provides a microcapsule and a preparation method thereof, a prepared feed and a feed production method, the microcapsule comprises a bacterial enzyme mixture, a sealing isolation layer, a pH response layer and a lipid outer layer; the bacterial enzyme mixture is arranged as a core, the peripheral side of the bacterial enzyme mixture is coated with the sealing isolation layer, the peripheral side of the sealing isolation layer is coated with the pH response layer, the peripheral side of the pH response layer is coated with the lipid outer layer, and the bacterial enzyme mixture comprises SOD compound enzyme containing Mn3O nanoparticles and a probiotic preparation. Through manganese-rich domestication and a multilayer embedding technology, the gastric acid tolerance of the microcapsule is improved. According to the present invention, the bacterial enzyme mixture prepared from the SOD compound enzyme containing the Mn3O nano-particles and the probiotic preparation can achieve the functions of bacteriostasis, oxidation resistance, growth promotion, deodorization and meat quality improvement, such that the characteristics of the SOD compound enzyme can synchronously improve the livestock and poultry muscle ratio and the meat quality so as to improve the comprehensive benefits of livestock and poultry breeding.

The invention provides a compound feed for promoting beef cattle growth and improving meat quality and a production method thereof, and belongs to the technical field of feed preparation. The compound feed comprises the following components in parts by weight: 100 parts of basal feed, 7-10 parts of a functional additive and 0.3-0.5 part of disodium inosinate, and the functional additive comprises a postbiotic compound, a fatty acid regulating agent, a bacterium enzyme synergist and trace elements, wherein the metagen complex comprises a lactobacillus plantarum metagen extract and a bacillus coagulans metagen extract, the fatty acid regulating agent comprises conjugated linoleic acid glyceride and medium-chain fatty acid, and the bacterial enzyme synergist comprises beta-mannase and bacillus subtilis. According to the compound feed, the growth performance and the meat quality can be synchronously improved, and a reliable processing technical guarantee is provided for improving the growth performance and the meat quality of beef cattle.

This invention discloses a quantitative detection kit for arginine peptidase, a qualitative detection kit for arginine peptidase, and their usage methods. The quantitative detection kit utilizes the specific substrate Nα-benzoyl-DL-arginine-4-ylbenzamide hydrochloride, which can be hydrolyzed by arginine peptidase to 4-ylbenzamide. 4-ylbenzamide has a maximum absorption peak at 405 nm, and the specific arginine peptidase activity can be rapidly detected at room temperature using spectrophotometry. The qualitative kit utilizes the specific substrate Nα-benzoyl-DL-arginine-β-naphthamide hydrochloride, which can be hydrolyzed by arginine peptidase to p-β-naphthamide. β-naphthamide combines with 4-(dimethylamino)cinnamaldehyde to form a compound that is visible to the naked eye as a pink color. The disulfide bond cleaving agent cuts the mucus disulfide bonds, exposing bacterial enzyme sites, and the synergistic effect of the enzyme activator enhances the reaction rate of arginine peptidase hydrolysis of the substrate, significantly improving the detection sensitivity of arginine peptidase in tongue coating samples.

This invention relates to the field of low-temperature fermentation formulation technology. The invention provides a low-temperature resistant compound bacterial enzyme formulation, its preparation method, and its application in the co-fermentation of straw and manure. The compound bacterial enzyme formulation comprises the following raw materials in parts by weight: 8-10 parts of compound bacterial agent, 1-1.4 parts of compound enzyme agent, and 88.6-91 parts of excipients. The compound bacterial enzyme formulation of this invention can ferment straw and manure under conventional conditions as well as at low temperatures of around 15°C, effectively meeting the fermentation requirements in low-temperature regions. By combining the excipients with the bacteria and enzymes, substances and nutrients during the fermentation process can be effectively adsorbed, avoiding acid inhibition, and providing attachment sites for the bacteria and enzymes, thus prolonging their activity.

The invention discloses a pig breeding feed composition based on bacterium-enzyme synergy. The pig breeding feed composition comprises a piglet feed, a growing pig feed and a fattening pig feed, the piglet feed, the growing pig feed and the fattening pig feed at least comprise bacillus coagulans, clostridium butyricum, phytase, amylase, trypsin and NSP enzyme, and the bacillus coagulans can produce lactic acid and bacteriostats to enhance the intestinal barrier function of bred pigs; the clostridium butyricum can produce butyric acid and can regulate the balance of intestinal flora; phytase can decompose phytic acid and release mineral substances such as phosphorus, calcium and zinc; amylase can hydrolyze starch into soluble sugar, so that the energy utilization rate is increased; trypsin is used for degrading protein into small peptide and amino acid, is adaptive to the high-protein requirement of bred pigs, and can degrade cell wall polysaccharide of plant feed, reduce chyme viscosity and release embedded nutrients together with NSP enzyme; the invention also discloses an application of the breeding feed composition for pigs in breeding pig products.

Disclosed is an additive manufacture material made from polymers and designed to be biodegradeable in a landfill or oceanic environment. The material may be made of bio-based polymers made from caster beans, cellulose, corn, starch, sugarcane, etc., such as nylon 11, bio-based polyethylene, polylactic acid, polyhydroxyalkanote, polyvinyl acetate, etc., to which is added microorganism, such as a bacteria, an enzyme or other additive to facilitate / accelerate the decomposition of the polymer in an environment where the object made through AM has been disposed, e.g., discarded after useful life. The microorganism or other additives that facilitate / accelerate the decomposition of polymers can also be added to petroleum-based, non bio-based polymers.

The present invention relates to an antimicrobial composite comprising a cationic antimicrobial compound and a bacterial enzyme degradable anionic copolymer wherein the bacterial enzyme degradable anionic copolymer and the cationic antimicrobial compound are linked to each other by electrostatic interaction. The invention also relates to a pharmaceutical composition comprising the antibacterial composite; a method of treating a subject suffering from a bacterial and / or fungal infection, comprising the step of administering to the subject a therapeutically effective amount of an antibacterial composite or pharmaceutical composition; the use of an antimicrobial composite in the preparation of a medicament for the treatment of bacterial and / or fungal infections in a subject in need of an antimicrobial composite; the use of the antibacterial composite or pharmaceutical composition for the treatment of bacterial and / or fungal infections; the invention also provides a method for preparing the antibacterial composite material.

The invention discloses a composite flora and activated mineral synergistic bio-organic fertilizer and a preparation method thereof. The preparation method comprises the following steps: crushing and mixing organic raw materials, adding a compound bacterium enzyme preparation, and sequentially carrying out two-stage temperature-controlled fermentation of high-temperature aerobic fermentation and medium-temperature curing to obtain a high-activity fermented material; the preparation method comprises the following steps: carrying out nano micropore activation treatment on monoclinoptilolite by using a hydrochloric acid solution to obtain activated zeolite with high specific surface area; and finally, mixing the fermented material with the activated zeolite in proportion, chelating at low temperature, granulating and drying to obtain the bio-organic fertilizer. Through composite flora construction, mineral nano activation and a step-by-step synergistic process, the effective viable count of the product reaches 2.0 billion CFU / g or above, the Pb < 2 + > adsorption rate exceeds 85%, and nutrient slow release and remarkable growth promoting effects are achieved. The product integrates the functions of soil improvement, disease inhibition, heavy metal restoration and nutrient slow release, and is suitable for ecological planting of various crops.

The invention provides a feed additive for increasing the content of n-3 polyunsaturated fatty acids in pork and a compound feed. The feed additive consists of spartina alterniflora powder and a bacterial enzyme additive, the bacterial enzyme additive is prepared from lactobacillus plantarum, aspergillus niger, yarrowia lipolytica, bacillus subtilis, cellulase, beta-mannase and protease; the compound feed comprises a basic ration and the feed additive. Through development and utilization of spartina alterniflora powder and practice, waste is turned into wealth, a new source of the feed additive is expanded, and the feed additive is added into daily ration, so that the digestibility of neutral detergent fibers of the daily ration is improved, the antioxidant capacity of serum is enhanced, and the contents of alpha-linolenic acid, arachidonic acid and eicosapentaenoic acid in pork are increased.

The preparation method comprises the following steps: uniformly mixing and stirring corn steep liquor, bran, bran shell powder and puffed soybean meal to obtain a mixture, and taking the mixture as a fermentation substrate; mixing the compound enzyme preparation and the compound probiotics to obtain a zymophyte enzyme agent; and inoculating the zymocyte enzyme agent into the fermentation substrate, uniformly mixing, sealing, standing and fermenting to obtain the fermented feed additive. The fermented feed additive is prepared from the corn steep liquor as a protein raw material by adopting a bacterium-enzyme synergistic fermentation technology, so that the problem that the feed protein raw material is high in price is relieved, the finished product of the fermented feed additive does not need to be dried, the production cost is low, and cost reduction and benefit improvement are facilitated.

The invention discloses a bacterium-enzyme synergistic microbial preparation and a preparation method and application thereof, and the bacterium-enzyme synergistic microbial preparation comprises a mixed bacterium suspension containing bacillus velezensis and bacillus altitudinis; the thallus concentration ratio of the bacillus velezensis to the bacillus altitudinis is (3: 1)-(1: 3); the mixed bacterial suspension also contains cellulase with the mass fraction of 0.5%-3%. After bacteria-enzyme synergistic fermentation treatment, the coordination of conventional chemical components of the tobacco leaves is improved. Moreover, after the bacteria-enzyme synergistic fermentation treatment, the volatile aroma components of the tobacco leaves are remarkably increased, and the content of the volatile aroma components such as megastigmatrienone, benzyl alcohol, phenethyl alcohol and damascenone is remarkably increased.

The invention discloses a multi-bacteria enzyme composition for water purification treatment and application, and relates to the technical field of water purification. The microbial agent is prepared from the following components in parts by weight: 15 to 30 parts of rhodopseudomonas palustris, 15 to 30 parts of bacillus subtilis, 25 to 35 parts of serratia marcescens, 25 to 35 parts of denitrifying pseudomonas, 25 to 35 parts of lactic acid coccus, 25 to 35 parts of bacillus amyloliquefaciens and 25 to 35 parts of rhodotorula benthica. The synergistic effect of the flora can inhibit the outbreak of harmful algae, promote the growth of beneficial algae, improve the flocculation performance of a water body and effectively improve the transparency. Meanwhile, the composition has a remarkable removal effect on key pollution indexes such as ammonia nitrogen, COD (Chemical Oxygen Demand), BOD (Biochemical Oxygen Demand), suspended solids and total phosphorus, so that the systematic repair of the water body is realized.

The present invention discloses a kind of bacterial enzyme synergistic debittering pea protein beverage and preparation method thereof, belong to the field of food processing technology.The method adopts pea protein as raw material, utilizes alkaline protease, flavor protease enzymolysis and plant lactobacillus, thermophilic streptococcus, Lactobacillus helveticus composite bacterial fermentation synergistic effect, compared with using lactic acid bacteria fermentation debittering time alone is shorter, overall debittering efficiency is higher, sensory evaluation is better, overall acceptance is high; The pea-related odor-related volatile compounds of the prepared product include hexanal, 2 nonanone, (E, E) 2,4 nonadienal, 2 amylfurans, (E, E) 2,4 decadienal etc. are reduced or completely eliminated.The debittering pea protein beverage provided by the present invention and preparation method thereof are conducive to broadening pea protein utilization approach and promoting the development of my country's pea protein beverage industry.

The invention relates to fermented soft granules for livestock and poultry and a preparation method thereof, and belongs to the technical field of livestock and poultry feed. The granular feed comprises the following components: 30-60% of starch, 10-30% of iced fresh fish, 10-30% of protein, 0.5-1% of vitamins, 0.5-3% of minerals, 2-5% of fat and 0.5-2% of compound bacteria enzyme. The granular material achieves the purposes of reducing the breeding cost, increasing the absorption and utilization rate, improving the immunity of bred animals and being free of resistance, promotes healthy breeding, guarantees food safety, and also can improve the eating taste and flavor of the bred animals and the food quality. The problems that an existing feed is low in production ratio, and the yield is not easy to increase are solved.

The invention belongs to the technical field of bacterium-enzyme synergistic fermentation methods, and particularly relates to a bacterium-enzyme synergistic fermentation method with high fermentation quality, which comprises the following steps: taking fresh moringa oleifera and morinda citrifolia stems and leaves, crushing the moringa oleifera and morinda citrifolia stems and leaves by a cutting machine until the particle size is 1-2cm, drying the crushed moringa oleifera and morinda citrifolia stems and leaves in the shade for 6 hours, and controlling the moisture content of the materials dried in the shade to be 65-75%, an enzyme microbial agent is added to the material dried in the shade and mixed to be uniform, the enzyme microbial agent is composed of lactic acid bacteria, bacillus licheniformis, aspergillus niger, cellulase, phytase and xylanase, and the dosage of the components is that the lactic acid bacteria accounts for 0.15% of the total mass of the material dried in the shade, the bacillus licheniformis accounts for 0.15% of the total mass of the material dried in the shade, the aspergillus niger accounts for 0.15% of the total mass of the material dried in the shade, the cellulase accounts for 0.1% of the total mass of the material dried in the shade, the phytase accounts for 0.1% of the total mass of the material dried in the shade, and the xylanase accounts for 0.1% of the total mass of the material dried in the shade. The materials mixed with the enzyme microbial inoculum are loaded into polyethylene silage fermentation bags, vacuum packaging and sealing are conducted, the sealed polyethylene silage fermentation bags are placed in a shady and cool place, and fermentation is conducted for 28 days.

The invention relates to the technical field of flavor agents, in particular to a cream type bacterium-enzyme co-promoted cheese flavor agent with good sensory characteristics and a preparation method of the cream type bacterium-enzyme co-promoted cheese flavor agent. The flavor agent provided by the invention is composed of hydrolase and active lactic acid bacteria, the hydrolase is prepared from Protease AY 'Amano' 400SD protease, the active lactic acid bacteria are prepared from lactococcus lactis BL-19 (preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC 16242), and the fermentation substrate is cream.

The invention provides a special Chinese herbal medicine and attapulgite compound fermented feed for cattle in a fattening period as well as a preparation method and application thereof, and belongs to the technical field of feed processing. The Chinese herbal medicine and attapulgite compound fermented feed is prepared from the following raw materials: radix astragali leftover materials, radix bupleuri leftover materials, silverweed cinquefoil root leftover materials, paper mulberry leaves, liquorice leftover materials, fennel leftover materials, corncobs, mixed-vitamin attapulgite clay and a bacterium enzyme mixture. The Chinese herbal medicines and the attapulgite clay are subjected to mixed fermentation by utilizing various microorganisms and enzymes, so that the digestion and utilization rate of nutrient substances of the raw materials of the feed can be increased, anti-nutritional factors can be passivated, the production performance and meat quality of meat / yaks can be effectively improved, the immunity of the meat / yaks can be improved, diseases can be reduced, and the feeding cost can be reduced; the use amount of antibiotics is reduced, and the problems of antibiotic residues and environmental pollution are reduced.

The invention relates to the technical field of biological fermentation, in particular to a method for synergistically fermenting distillers' grains by bacteria and enzymes. The viable count of the three functional bacteria provided by the invention can reach 107CFU / g or above after the three functional bacteria are cultured for 5 days in an environment with the temperature of 50-60 DEG C and the pH value of 3.0-4.0. In the environment, indigenous flora naturally carried by the yellow wine lees are difficult to survive, interference of indigenous bacteria can be basically eliminated in the fermentation process, growth and reproduction of functional bacteria are facilitated, and the stability of large-scale fermentation can be improved. The bacterial enzyme synergistic process activates the metabolic diversity of microorganisms through enzymolysis, the content of propionic acid and acetic acid is remarkably increased, meanwhile, succinic acid is kept stable, and the antibacterial activity and metabolic balance of an organic acid spectrum can be optimized. Meanwhile, enzymolysis can promote degradation of medium-chain fatty acid, and enzymolysis continuously optimizes a lipid metabolism structure in the later stage. The proline content of a bacterium-enzyme synergistic fermentation group is far higher than that of a pure fermentation group, so that the palatability of the feed is remarkably improved. Moreover, methionine is kept stable in a bacterium-enzyme synergistic group, so that nutrition loss can be avoided.

The present invention relates to the field of feed additives, specifically a bacterial enzyme peptide feed additive for preventing pathogenic stunted cow disease and stunted sheep disease, and a preparation method thereof. The present invention uses soybean meal, corn stalks, wheat bran, and rapeseed meal as raw materials, and pre-treats the raw materials by crushing, acid, and alkali to obtain pre-enzyme hydrolysis raw materials; the obtained pre-enzyme hydrolysis raw materials are mixed with coral mucus, and enzymolyzed with a composite enzyme to obtain an enzymatic solution; the enzymatic solution is then inoculated with a composite fermentation bacteria for fermentation to obtain a fermentation liquid; and finally, the fermentation liquid is spray-dried to obtain a bacterial enzyme peptide feed additive for preventing pathogenic stunted cow disease and stunted sheep disease with high stability, good preventive effect against pathogenic stunted cow disease, and low cost.

The present application relates to a kind of high conversion rate degradation cotton stalk free gossypol enzyme combination, in view of the existing cotton stalk free gossypol strong toxicity, low degradation efficiency, lead to its resource utilization is limited, the present application obtains efficient degradation strain and compound specific enzyme preparation, constructs and forms bacteria enzyme combination system.The bacteria enzyme combination includes degradation strain fermentation liquor and composite enzyme preparation, wherein the degradation strain is the mixed strain of pseudomonas and bacillus, and the composite enzyme preparation is compounded by laccase, peroxidase and esterase according to a specific ratio.The degradation rate of the combination to free gossypol in cotton stalk can reach more than 95%, which is significantly better than the degradation effect of single strain or single enzyme preparation.

The invention discloses a compound bacterium enzyme preparation for efficiently degrading shrub lignocellulose and application of the compound bacterium enzyme preparation, and relates to the technical field of agricultural microorganisms. The preparation consists of efficient lignocellulose degrading bacteria obtained by screening and a compound enzyme system (cellulase, xylanase and laccase in a ratio of 5: 3: 2) in a specific ratio, and is prepared by an optimized fermentation process. The viable count of the product is greater than or equal to 1 * 10 < 8 > CFU / g, neutral detergent fibers and acid detergent fibers of shrubs can be respectively reduced by 6.4% and 8.27%, the in-vitro dry matter digestibility is improved by 13%, and the product can be stably preserved for more than 6 months at room temperature. The preparation method comprises fermentation liquor preparation and shrub raw material treatment, is simple and convenient to use, can significantly improve the nutritional value of the shrub feed, reduces the breeding cost, and provides an economic and effective solution for the feed utilization of shrub resources in desertification regions.

This invention belongs to the field of component detection technology and discloses a method and application for the detection of octadecyl vinyl ether. Using ginseng prepared by co-fermentation of ginseng powder and bacterial enzymes as the sample, a three-step pretreatment method is employed: ethanol extraction combined with liquid-liquid extraction of a toluene:n-hexane mixture (5:1 volume ratio) and PDMS solid-phase microextraction. This is combined with gas chromatography-tandem mass spectrometry (GC-MS) to achieve qualitative and quantitative analysis of octadecyl vinyl ether. The three-step pretreatment method uses ethanol extraction combined with liquid-liquid extraction of a toluene:n-hexane mixture and PDMS solid-phase microextraction. The ethanol extractant is consistent with the solvent used in the preparation of fermented ginseng, ensuring sufficient dissolution of the target analyte. The toluene-n-hexane mixed solvent efficiently separates the target analyte from polar matrix impurities, and the boiling point difference facilitates subsequent solvent removal. PDMS solid-phase microextraction further enriches trace amounts of the target analyte. Compared with the reduced pressure concentration method, the detection sensitivity is improved by more than 2 times, and matrix interference is removed more thoroughly.

The invention relates to the technical field of bamboo treatment, and discloses a bamboo crude fiber bacterial enzyme decomposition method which comprises the following steps: S1, preparing raw materials; s2, cultivating synthetic bacteria enzyme; s3, a first stage of fermentation; step S4, a second stage of fermentation; and S5, a third stage of fermentation. According to the bacterial enzyme decomposition method for the bamboo crude fibers, bamboo decomposition bacterial enzyme groups are cultivated in a synthetic biological mode, indigestible components such as the bamboo crude fibers of animals are converted into nutrient substances easy to absorb, the nutritive value and digestibility of feed using the bamboo components are improved, use of antibiotics in the feed is greatly reduced, and the production cost of finished feed is reduced; the method has the advantages that requirements on staple food grains of traditional feed raw materials are reduced, imported forage grass is reduced, breeding cost is reduced, effective components and probiotic living bodies in bamboos can be utilized by the aid of synthetic biological bacterium enzyme technologies, and intestinal microecological balance of livestock and poultry, fish, shrimp, crab, sea cucumber and shellfish can be effectively conditioned in a manner of developing resistance-reducing feed by the aid of bamboos instead of food grains.

The invention belongs to the crossing field of nanotechnology, synthetic biology and sewage treatment, and particularly relates to a hydroxylamine oxidation-reduction nano-enzyme, a bacterium-enzyme compound and application of the hydroxylamine oxidation-reduction nano-enzyme and the bacterium-enzyme compound in nitrite synthesis. The hydroxylamine redox nano-enzyme (Ir-coated PVP nano-enzyme) is obtained through a solvothermal method reaction of an iridium salt precursor and polyvinylpyrrolidone in an organic solvent, the molar ratio of the iridium salt precursor to the polyvinylpyrrolidone is (10-100): 1, and the Ir-coated PVP nano-enzyme can catalyze substrate hydroxylamine to be oxidized into nitrite. The engineering strain with the novel ammoxidation capacity is obtained through modification, ammonia nitrogen in culture or sewage can be converted into hydroxylamine, and a catalytic substrate is provided for nano-enzyme. The nano-enzyme is high in catalytic efficiency and stable in activity, and the use cost can be saved. Therefore, the Ir-coated PVP nano-enzyme and bacterial enzyme compound constructed by the invention has huge social benefits, economic benefits and good industrial application prospects.