48 results about "Viable Cell Count" patented technology

The invention discloses microorganism-containing compound fertilizer and a preparation method thereof. The microorganism-containing compound fertilizer has a water content of less than or equal to 5.0%; based on a mass fraction, the total nutrient of nitrogen, phosphorus and potassium is more than or equal to 35%, and the effective viable cell count is not less than 2.0*10<7> CFU/g; the compound fertilizer is mainly prepared from 10-40% of urea, 10-35% of monoammonium phosphate, 10-40% of ammonium sulfate, 10-40% of potassium sulfate, 10-35% of potassium chloride, 2-15% of calcium-magnesium-phosphate fertilizer, 2-15% of clay, 0.3-0.5% of coating material and 0.01-1% of microbial powder. The preparation method provided by the invention effectively avoids the influence of high temperature on effective viable counts of microorganisms in a fertilizer production process; the yield, single plant weight and Vc content of lettuce treated by using the compound fertilizer provided by the invention are generally higher than those of lettuce treated by using the common compound fertilizer; the effect of the compound fertilizer is significantly higher than that of the similar products in the market; the compound fertilizer can increase the yield and improve the quality, maintains the survival rate of the microbial agent, and has certain stability.

The invention provides a process of preparing fermented milk beverage keeping high viable cell count at ambient temperature. The process includes the steps of adding regular yoghurt lactobacillus into milk for fermentation, fermenting until pH value to 3.8-4.8, diluting, mixing and sterilizing with conventional method, adding concentrated culture, concentrated frozen culture or freeze dried culture containing Lactobacillus rhamnosus ATCC 53103 into mixed milk beverage at aseptic condition. According to various pH value of finished product, it can be stored at ambient temperature for 1-6 months, and the viable cell count will not be less than 10⁵ cfu/ml milk beverage. The indexes of storage period and viable cell count are much higher than fermented milk beverage produced by conventional process. Thereby the defect that fermented milk beverage has short shelf life and low viable cell count at ambient temperature is overcome efficiently.

An object of the invention is to provide a lactic acid bacteria fermentation product, which has been obtained by culturing lactic acid bacteria on a medium containing an extract of at least one food material selected from the group consisting of rice bran, persimmon leaves, perilla, Houttuynia cordata Thunb, Eucommia ulmoides Oliv., turmeric, clove, cinnamon and Rubus suavissimus S. Lee (Rosaceae). By adding and mixing the extract used for the preparation of the fermentation product to a medium, it is possible to increase simply the viable cell count of lactic acid bacteria, without affecting the flavor of the product. It is possible, by using the extract, to obtain a lactic acid bacteria fermentation product which contains many viable lactic acid bacteria with their activities highly maintained, and further to provide beverages or foods using the product.

The invention relates to a cell viability detection kit and a preparation method and an application thereof. The invention is applicable to live cell counting, and is a determining method for directly detecting the cell proliferation, and is also a detection method for estimating the cell totoxicity of the drugs, peptide growth factors and other substances, and is applicable to detect the suspended cell and the anchorage-dependent cell cultured in the cell culture plates. The invention consists of a WST-8 mixed solution storage solution, a reaction stop solution and a buffer diluent solution, wherein the WST-8 mixed solution storage solution contains 50mmol/L WST-8 solution, 2mmol/L PMS and 1mmol/L PIPES buffer solution; the reaction stop solution is 10%(W/V) sodium dodecyl sulfate; and the buffer diluent solution is 1mmol/L PIPES solution.

Clogging of membrane by slime adhesion is efficiently prevented and stable treatment can be carried out for a long period of time at a low cost by a small amount of chemicals without the membrane deterioration and trihalomethane formation even if applied to the water having a large number of viable cell counts and having water quality of harsh. The method comprises supplying water to be treated to a membrane separation apparatus 4, adding intermittently to the water to be treated a combined chlorine agent comprising sulfamic compound, and repeating a non-addition feeding period in which water to be treated is supplied for 6-120 hours without addition of combined chlorine agent, and an intermittent addition feeding period in which water to be treated is supplied for 0.5-40 hours under addition of a combined chlorine agent at biofilm exfoliating concentration in an early stage of biofilm formation during the non-addition feeding period, wherein viable cell count (log CFU/mL) of water to be treated is 3 or more, and the concentration of the combined chlorine agent in the water in the intermittent addition feeding period is 0.5-20 mg/L as total chlorine, and additive amount of the combined chlorine agent added in the intermittent addition feeding period is the amount in which R represented by the following Formula [4] is 3 or more.

R=(Intermittent addition feeding period (h)×[1000×Intermittent addition concentration (mg-Cl/L)]^2.5/(Non-addition feeding period (h)^{3.0×10log CFU/mL})  [4]

The invention relates to composite microbial agents, in particular to a composite microbial agent for water environment treatment and an application thereof. The composite microbial agent comprises: Pseudomonas otitidis, Pseudomonas kunmingensis, Pseudomonas asiatica, and Alcaligenes sp., and the total viable cell count of the microbial agent is not less than 1 x 10<9> cfu/mL. The composite microbial agent has low cost and no pollution to environment, a polluted water body is treated at an adding ratio of 0.01%-0.1%, after 1-2 weeks, removal efficiency of ammonia nitrogen is close to 100%, andtotal nitrogen elimination is up to 60% or more. At the same time, no accumulation of nitrites and nitrates is caused, the functional strain is traced in situ after repairing is completed, and thus relatively strong adaptability of the microbial agent in an actual water environment is verified. The composite microbial agent can be used for water environment treatment, and a feasible method for elimination of black odorous water body in China is provided.

The invention provides a composite microecological preparation and a preparation method and application thereof. The composite microecological preparation is prepared from lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae. The composite microecological preparation as a feed additive for laying hens has a higher viable cell count, has the advantages of improving the productionperformance of the laying hens, delaying the decline of an egg production rate, and improving the quality of eggs, and has broad application prospects and great market value.

The present invention relates to a mixed inoculant agent for effective degradation of mushroom bran. The mixed inoculant agent is prepared by mixing a straw fermentation broth with a crude fiber degrading broth by 1:5-5:1 to prepare a mixed inoculant stock solution, then inoculating 0.25%-1.0% of the mixed stock solution into sterile distilled water, adding a mixed broth containing Aspergillus niger and Penicillium and accounting for 1.0-5.0 wt.% of the sterile distilled water, and stably culturing for 2-3 d. The preparation method of the mixed broth is as follows: inoculating Aspergillus niger and Penicillium to a PDA slant medium for 3-4 d respectively, washing the spores on the slant with sterile water to prepare a spore suspension with viable cell count of 106 CFU/mL; and conducting liquid fermentation culture on the Aspergillus niger and Penicillium according to the proportion of 15%-25% for 7-8 d to obtain a mixed fermentation broth. The mixed inoculant agent of the present invention to is convenient for the mushroom bran degradation, and has obvious degradation effect.

A rapid method for the quantitation of various live cell types is described. The method may include a variety of steps including: 1) suspending the cells in a detergent-like compound, 2) isolating the washed cells by centrifugation or filtration, 3) resuspending the cells in a solution that contains a preservative, a fluorescent dye and a compound such as dequalinium which can be taken up by the cells, 4) measuring the fluorescence increase over time of the cell-dye mixture with a simple fluorometer, and 5) measuring the native fluorescence of the cells. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.

The invention relates to the field of biology, in particular to a composite microbial treatment agent for fermenting silage and hay silages and a preparation method thereof. The composite microbial treatment agent is characterized by being prepared from, by weight, 10-30 parts of lactobacillus plantarum, 15-30 parts of bacillus coagulans, 15-30 of lactobacillus rhamnosus and 10-30 parts of lactobacillus buchneri, and the effective viable cell count of the microbial treatment agent is 1.0 to 9.9*109 CFU/mL. Compared with the prior art, the method uses the bacillus coagulans, the lactobacillus plantarum, the lactobacillus rhamnosus and the lactobacillus buchneri composite microbial agent for silage/hay silage fermentation, and can effectively inhibit the growth of hybrid bacteria, after fermentation, the pH can be increased to 3.7-4.1, the total organic acid content such as acetic acid, propionic acid and butyric acid is increased, the ammonia nitrogen content is reduced, a finished product has an aromatic fruit flavor, thereby reducing a taste beneficial to improving the feeds, and the nutritional value of the feeds is improved.

The invention discloses a straw decomposing agent which comprises components of Paenibacillus macerans CGMCC No. 14564 and Bacillus subtilis CICC No. 10089; the total effective viable cell count in the decomposing agent is 0.5*10<8>-2*10<8>/g, and the effective viable cell count of the Paenibacillus macerans is 65%-70% of the total effective viable cell count; the invention also discloses a preparation method of the straw decomposing agent, compared with use of various strains to prepare the decomposing agent in the prior art, the preparation method has a small variety of raw materials, and has a simple preparation process, the Bacillus subtilis whoch is not used in the prior art is used in the preparation method as one of the raw materials to interact with the Paenibacillus macerans, andthe use effect is remarkable, so that the preparation method can be promoted in related enterprises.

To shorten a long aging time required for meat processing and reducing the viable cell count of a carcass before being cooked, an electric stimulus is imparted by applying an AC voltage via from electrode needles to at least the neck and legs of the meat carcass. The AC voltage is turned on for a time Ton and then turned off for a time Toff, and the application of the voltage is repeated a predetermined number of cycles. The Ton period is not longer than the Toff period, although the first Ton period can be longer than the Toff period. The voltage is not higher than 100V, and the frequency is not higher than the commercial frequency (maximum, 60 Hz). The aging effect is improved by stimulating the entire meat carcass electrically from at most three points.

The invention discloses a preparation method of multifunctional ready-to-eat fermented yoghurt powder. Raw material milk is prepared according to a specific ratio; introduction of a preferred freeze-drying carrier effectively ensures the efficient effects of the yoghurt powder in a viable cell count, brewing reconstitution properties and a preservation period, and ensures that the yoghurt powder has high nutritional value, good solubility, high stability, and delicate taste after brewing; and in addition, flavor functional powder is prepared independently, so that flavor and nutritional functions can be changed according to consumer preferences and desired functions, and therefore, the multifunctional ready-to-eat fermented yoghurt powder has good market prospects. The multifunctional ready-to-eat yogurt powder disclosed by the invention has a protein content of 30.38-48.25% and a fat content of 5.6-11.3%.

A cell testing device according to one aspect of the present invention includes: an impedance sensor configured to measure impedance of a culture solution; a storage unit configured to store a coefficient for estimating the number of viable cells present in the culture solution during a prescribed period using the impedance for each of a plurality of periods into which the prescribed period within a culture period from the start of cell culture to death is divided; and a viable cell count estimation unit configured to acquire the impedance and estimate the number of viable cells using at least one of the acquired impedance and a coefficient for each period stored in the storage unit.

The invention relates to a lactobacillus plantarum preservative for a low-salt meat product. A strain for the lactobacillus plantarum preservative is lactobacillus plantarum, and is preserved in the CGMCC (China General Microbiological Culture Collection Center). The lactobacillus plantarum preservative is a light white semitransparent suspended bacterium fluid; the viable cell count of the lactobacillus plantarum preservative is 109 CFU/mL; the lactobacillus plantarum preservative is uniformly inoculated on the surface of the low-salt meat product by adopting a spraying method; and the inoculated low-salt meat product is quickly packed in vacuum, and is stored at the temperature of 2 to 6 DEG C. The lactobacillus plantarum preservative remarkably inhibits the growth of putrefying bacteriatherein, delays the fat oxidation of the product, and prolongs the shelf life of the low-salt meat product.

The present invention provides means for evaluating harmfulness of a chemical substance before occurrence of cell death, that is, more quickly and sensitively compared to conventional methods wherein the remaining viable cell count is determined using a reductive coloring reagent after a period of time required for occurrence of cell death due to the chemical substance. A double-stranded RNA probe comprising an RNA strand labeled with a fluorescent dye A that emits fluorescence, and an RNA strand labeled with a fluorescent dye B that quenches emission from a fluorescent dye in the vicinity thereof, wherein the fluorescence is quenched in a double-stranded state due to occurrence of fluorescence resonance energy transfer (FRET) between the two kinds of fluorescent dyes. When the probe is introduced into a cell and the cell is in a normal state, the double-stranded RNA is quickly degraded by activity of intracellular ribonuclease to cause cancellation of the FRET state, allowing fluorescence emission of the fluorescent dye A. On the other hand, in cases where the cell is harmfully influenced, activity of intracellular ribonuclease is suppressed, so that the double-stranded RNA remains undegraded, and the fluorescent dye A remains quenched due to the influence of the fluorescent dye B. By detecting a decrease in the degradation rate of intracellular RNA based on this principle, harmfulness of a chemical substance can be quickly and sensitively evaluated before occurrence of cell death.