47 results about "Viable Cell Count" patented technology

The present invention relates to a composite microbial agent and its use in the remediation or management of chlorinated hydrocarbon contaminated sites. The composite microbial agent comprises composite microorganisms, wherein the composite microorganisms include Gordonia, Pseudomonas, Pseudomonas, and Terrestria in a viable cell count ratio of 100:(25-600):(10-900):(10-500). The present invention effectively addresses the difficulty in remediating chlorinated hydrocarbon contaminated sites, overcomes the problems of low mass transfer efficiency and limited microbial activity of traditional remediation technologies, and improves the efficiency of remediation and risk management of chlorinated hydrocarbon contaminated sites. The agent is particularly suitable for green and efficient remediation of soil and groundwater in silty soil sites, pollution source reduction, and risk management. Furthermore, the agent offers advantages such as low cost and ease of operation, and thus has significant application prospects.

This invention discloses a multifunctional Pediococcus pentosaccharide strain and its applications. Pediococcus pentosaceus The strain, named *Pediococcus pentosaceus* A07, has the accession number CCTCC NO: M 2025777 and is deposited at the China Center for Type Culture Collection (CCTCC), located at Wuhan University, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province. This strain is capable of degrading nitrite. Using *Pediococcus pentosaceus* A07 to reduce nitrite content can produce high-quality pickles with good flavor and texture, low nitrite content, and high viable cell count. The *Pediococcus pentosaceus* A07 provided by this invention also possesses cholesterol-lowering, zinc protoporphyrin-forming, antibacterial, and antioxidant capabilities, and produces a high biofilm.

The present invention provides a method for modulation of afucosylation level of Pertuzumab by using pH shift of cell culture media, when cell culture achieves viable cell count (18-22) x 106 cells / mL.

The present invention discloses a fusion strain of Lactobacillus paracasei and Bifidobacterium adolescentis, as well as its preparation method and application, belonging to the field of strain breeding technology. A fusion strain of Lactobacillus paracasei and Bifidobacterium adolescentis was successfully prepared using the method provided by the present invention. The prepared fusion strain has a new morphology, a broadened carbon source utilization range, and significantly improved inhibitory effects on three pathogens. Furthermore, it is resistant to both vancomycin and mupirocin lithium salts. It also has good fermentation characteristics, with a pH of 4.05 and a titer of 114°T after 28 days of storage, and the viable cell count can still be maintained at 1×10 7 The protoplasts have a CFU / mL value of above 2260 mPa·s, a viscosity value of 37.462 g, a hardness of 37.462 g, and a consistency of 282.04 g·s, and can be used for the development and application of dairy products. The preparation method provided provides protoplasts with strong regeneration ability, high vitality, and high fusion rate, providing support for the selection of excellent lactic acid bacteria strains using protoplast fusion technology.

The invention discloses a multi-strain active lactobacillus beverage and a preparation method thereof. The multi-strain active lactobacillus beverage disclosed by the invention is good in flavor and mouth feel, fresh and cool in mouth feel, sour, sweet and delicious, and capable of maintaining the constant viable count within the shelf life and still maintaining the viable count gt after being refrigerated at 2-10 DEG C for 30 days; the method has the advantages of high purity, high purity, 1 * 10 < 9 > CFU / ml, good stability, no layering and precipitation phenomena, and centrifugal precipitation rate maintained within 2%.

This invention belongs to the field of microbial fermentation technology, specifically relating to a method for preparing microbial inoculants through mixed fermentation of Bacillus subtilis and Bacillus cereus. The method includes: separately activating Bacillus subtilis and Bacillus cereus to obtain a seed culture; adding a basic fermentation medium to a fermenter, first inoculating Bacillus subtilis, and then inoculating Bacillus cereus after it enters the mid-logarithmic growth phase, achieving sequential mixed inoculation; employing segmented temperature control, two-stage pH control, and dynamic sugar feeding during fermentation, while simultaneously adding malic acid in a coupled manner between 20 and 35 hours to alleviate oxidative stress; collecting the cells after fermentation, adding a protective agent, and drying to obtain the inoculant. This invention, through the synergistic effect of sequential inoculation and malic acid coupled feeding, achieves an effective viable cell count of up to 1.0 × 10⁻⁶. 13 With a CFU / mL or higher, the spore formation rate is ≥89%, which is significantly better than the existing mixed-strain fermentation process.

The invention discloses a composite flora and activated mineral synergistic bio-organic fertilizer and a preparation method thereof. The preparation method comprises the following steps: crushing and mixing organic raw materials, adding a compound bacterium enzyme preparation, and sequentially carrying out two-stage temperature-controlled fermentation of high-temperature aerobic fermentation and medium-temperature curing to obtain a high-activity fermented material; the preparation method comprises the following steps: carrying out nano micropore activation treatment on monoclinoptilolite by using a hydrochloric acid solution to obtain activated zeolite with high specific surface area; and finally, mixing the fermented material with the activated zeolite in proportion, chelating at low temperature, granulating and drying to obtain the bio-organic fertilizer. Through composite flora construction, mineral nano activation and a step-by-step synergistic process, the effective viable count of the product reaches 2.0 billion CFU / g or above, the Pb < 2 + > adsorption rate exceeds 85%, and nutrient slow release and remarkable growth promoting effects are achieved. The product integrates the functions of soil improvement, disease inhibition, heavy metal restoration and nutrient slow release, and is suitable for ecological planting of various crops.

To provide a method for appropriately setting the intensity of ultraviolet rays at a flow rate at which optimum power efficiency is obtained in order to prevent infection to individuals in other water tanks even if an individual in one of a plurality of water tanks develops an infectious disease in a water circulation system.SOLUTION: The management device 100 is configured to, based on a sterilization target value including a safe maximum viable cell count and an allowable immersion time, bacteria information including a viable cell count increase amount indicating the number of bacteria released into water per unit time by the aquatic organisms having developed infectious diseases and ultraviolet light sensitivity information of bacteria to be sterilized, and rearing condition information including a plurality of water tank 20A, a water amount of 20B, the number of water tanks, and a lower limit flow amount of a pump, A specifying unit configured to set an ultraviolet target intensity of the ultraviolet light source so that the number of viable bacteria in at least one of the plurality of water tanks falls below the safe maximum number of viable bacteria within the allowable immersion time when the set flow rate of the pump is set to a target flow rate based on a lower limit flow rate.SELECTED DRAWING: Figure 1

The invention provides bacillus velezensis and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis ND-J with a remarkable inhibition effect on main pathogenic bacteria of the ginger root rot is successfully obtained, and the fermentation viable count of the bacillus velezensis ND-J is greatly increased through system optimization, so that a reliable strain resource and a mature fermentation process foundation are provided for developing an efficient and stable ginger root rot biological control preparation. The application of the technology is helpful to reduce the use of chemical pesticides, promotes the green and sustainable development of the ginger industry, and has positive significance in guaranteeing the yield and quality of ginger and increasing the economic benefits of ginger farmers.

The application discloses an application of astragalus polysaccharide in promoting lactic acid bacteria metabolism and improving quality and stability of fermented products, and belongs to the technical field of fermented dairy product processing. The application adds 2 ‰ to 10 ‰ of astragalus polysaccharide and sucrose in skimmed milk with respect to the mass of the skimmed milk, inoculates lactic acid bacteria starter after pasteurization, and constant-temperature fermentation is carried out until the end point to prepare fermented products. The application utilizes the dual functions of astragalus polysaccharide in promoting lactic acid bacteria metabolism and strengthening milk protein gel network to realize the synergistic improvement of quality and stability of fermented products. Experiments show that when the addition amount of astragalus polysaccharide is 4 ‰, the sensory score of the fermented product reaches 87.3, and the water holding capacity is increased by 11.95 % compared with the control group; scanning electron microscopy shows that the gel network is dense and has few pores; when the addition amount is 6 ‰, the viable cell count reaches 1.23 x 10 8 CFU / ml; and when the addition amount is 8 ‰, the texture characteristics are optimal. The application is simple in operation, suitable for industrial production, and provides a new way for the development of functional fermented dairy products.

The present disclosure relates to: a culture medium composition for culturing lactic acid bacteria, which comprises superfine red pepper powder as an active ingredient; a method for culturing lactic acid bacteria using the culture medium composition for culturing lactic acid bacteria; a method for increasing a viable cell count of lactic acid bacteria; a lactic acid bacteria fermentation broth prepared using the culture method; a probiotic food composition comprising the lactic acid bacteria fermentation broth; and a probiotic beverage composition comprising the lactic acid bacteria fermentation broth.

The invention belongs to the technical field of biology, and particularly relates to a plant lactobacillus FMBL L23251FJX and application thereof.The plant lactobacillus FMBL L23251FJX is preserved in the China Center for Type Culture Collection on June 26, 2023, the preservation number is CCTCC NO: M20231100, and the plant lactobacillus FMBL L23251FJX has the advantages of being resistant to oxidation, resistant to acid, sensitive to antibiotics and good in antibacterial performance and has a good application prospect. The lactobacillus plantarum FMBL L23251FJX is compounded with prebiotics to prepare yoghourt, and the obtained prebiotic yoghourt is high in viable count in the storage period, obviously improved in acidity and sensory score, richer and richer in taste, high in volatile flavor substance content and wide in application prospect.

Disclosed herein are impedance-based biosensor systems comprising an impedance-based biosensor having an electrode, a meter in electrical communication with the electrode, a processor in electrical communication with the impedance-based biosensor and the meter, and a memory storing instructions to be executed by the processor. The instructions can cause the impedance-based biosensor system to measure the impedance of the electrode, derive one or more electrical properties of the impedance-based biosensor from the impedance of the electrode, and calculate a viable cell count based on the one or more electrical properties.

The invention discloses bacillus licheniformis YHD2 and application thereof in degrading nitrite and inhibiting aquatic pathogenic bacteria, and belongs to the technical field of microorganisms. The preservation number of the bacillus licheniformis YHD2 is CCTCC NO: M 20251259, and the bacterial colony of the bacillus licheniformis YHD2 is milk white, opaque, irregular in edge and rough in surface; the viable count of the prepared bacterial powder is at least 1 * 10 < 9 > CFU / g, and the bacillus licheniformis YHD2 has the capability of rapidly degrading nitrite and the capability of inhibiting aquatic pathogenic bacteria. Experiments prove that the novel bacillus licheniformis YHD2 has important significance on development of aquaculture water quality purification and preparation of a bacteriostatic agent for aquatic pathogenic bacteria, the application range of the bacillus licheniformis YHD2 is further widened, and the bacillus licheniformis YHD2 has wide economic value and development prospects in aquaculture.

The invention discloses a fermented feed additive for replacing soybean meal as well as a preparation method and application of the fermented feed additive. The fermented feed additive disclosed by the invention is prepared by performing acid protease enzymolysis on a mixed fermentation raw material and fermenting the mixed fermentation raw material with a complex microbial inoculant, wherein the mixed fermentation raw materials comprise the following components in parts by weight: 40-80 parts of corn protein powder, 30-60 parts of corn steep liquor, 7-12 parts of corn germ meal, 4-15 parts of molasses and 2-5 parts of extruded soybeans; the complex microbial inoculant is composed of pediococcus acidilactici, candida utilis and bacillus subtilis according to the viable count ratio of (1-3): (1-3): (0.1-1). The fermented feed additive can effectively replace soybean meal in animal daily ration, the utilization rate of nutrients by animals is increased, the health level of the animals is improved, and the production performance of the animals is improved.

The invention provides a culture medium additive for promoting growth of bacteroides microorganisms and application of the culture medium additive. The culture medium additive comprises an organic carbon source, an organic nitrogen source, an inorganic nitrogen source, magnesium salt, a regulator and a reducing agent. By adding the culture medium additive into a commercial culture medium, the rapid growth of bacteroides can be promoted, the viable count is increased, the culture period is shortened, a thought can be provided for the research on high-density fermentation of bacteroides in the future, and the research and development period is shortened.

The invention provides heat-resistant pediococcus acidilactici and application of the heat-resistant pediococcus acidilactici in pellet feed production. The heat-resistant pediococcus acidilactici is named as pediococcus acidilactici YJ and is preserved in the China Center for Type Culture Collection on May 19, 2025, and the preservation number of the heat-resistant pediococcus acidilactici is CCTCC (China Center for Type Culture Collection) NO: M 20251096. The strain YJ has heat-resistant and extrusion-resistant functions, and can be added into a feed production process before particle forming to obtain a feed product with high viable count and uniform addition, so that the growth and intestinal development of chicks are promoted, intestinal injury is repaired, and the production performance of the chicks is improved. The strain YJ can tolerate the high temperature of 90 DEG C, and can be directly added in a mixing stage, so that the strain YJ can be uniformly added in powdery, hard pellet feed and expanded pellet feed, meanwhile, the magnitude order of viable count can reach 107 cfu / g feed, and meanwhile, the variable coefficient of mixing uniformity is less than 5.0%.

The application provides a grape juice enzyme fermentation method and application, and relates to the technical field of fermentation.The grape juice enzyme fermentation method provided by the application comprises the following steps: inoculating fermentation bacteria in a culture medium containing grape juice to perform fermentation, and preparing grape juice enzyme; the fermentation bacteria comprise lactobacillus plantarum Zhang-LL and kloeckera apiculata M3; the preservation number of the lactobacillus plantarum Zhang-LL is CGMCC No.6936; and the preservation number of the kloeckera apiculata M3 is CGMCC No.1811.The fermentation method uses the lactobacillus plantarum Zhang-LL and the kloeckera apiculata M3 fermentation bacteria, and through the synergistic cooperation of the two, the fermentation time is shortened, and a high viable cell count can be achieved; the grape juice enzyme can be significantly improved in terms of antioxidant property and total protease activity; and the application provides a theoretical basis for industrialized preparation of grape enzyme and subsequent research and development of multifunctional products.

This invention discloses a high SOD activity *Gnaphalium affine* juice, a *Gnaphalium affine* juice probiotic fermented product, and its preparation method. Using *Gnaphalium affine* as raw material, this invention obtains a high SOD activity *Gnaphalium affine* juice through a combination of repeated low-temperature treatment, pulping, ultrasonic crushing pretreatment, and heat treatment. Fermentation is carried out using *Lactobacillus mucilaginosus* Grx07, with the addition of a commercial fermenting agent to regulate the fermentation process. This achieves a synergistic effect between probiotics and *Gnaphalium affine* juice in terms of SOD activity, improving the SOD activity, viable cell count, and sensory quality of the fermented product. It has broad application prospects in the preparation of probiotic fermented foods with high antioxidant activity.

The invention relates to the technical field of soil improvement. The invention provides a saline-alkali soil improving microbial agent and a preparation method thereof. The saline-alkali soil improving microbial agent is prepared from the following raw materials in parts by weight: 15 to 20 parts of bacillus megatherium, 10 to 15 parts of bacillus subtilis, 8 to 12 parts of bacillus mucilaginosus, 5 to 8 parts of pseudomonas fluorescens, 6 to 10 parts of trichoderma harzianum, 5 to 8 parts of arbuscular mycorrhizal fungi and 3 to 5 parts of rhizobium. Through cooperative use of the strains, the salt content of the coastal salinized soil can be effectively reduced, the pH value can be adjusted, the nutrient index of the salinized soil can be improved, the content of microorganisms in the soil can be improved, and the root development of crops can be promoted. Meanwhile, the sectional fermentation process can solve the problem of strain antagonism, and the salt tolerance and viable count of the strain are improved in cooperation with a special composite culture medium, so that the saline-alkali soil improvement effect is further enhanced.

The application discloses application of Pseudomonas pseudoalcaligenes 94-6Y in removing pollutants in wastewater, and belongs to the technical field of microorganisms. The Pseudomonas pseudoalcaligenes 94-6Y has the preservation number of CGMCC No.32718, has the characteristic of tolerating penicillin, can grow in penicillin wastewater with the concentration of 0.1-2.5 mg / L, and the effective viable cell count can reach 5x10 8 CFU / mL after 72 hours in the penicillin-containing wastewater, and it is also proved that the Pseudomonas pseudoalcaligenes 94-6Y has the characteristic of efficiently reducing pollutants in wastewater, wherein the removal rate of chemical oxygen demand reaches 68.41%, the removal rate of ammonia nitrogen reaches 85.92%, and the removal rate of total phosphorus reaches 21.3%, which has a significant effect on improving the water quality of tail water of antibiotic production industry and reaching the discharge standard.

The present invention discloses a fermentation-assisted fruit and vegetable flavored beverage production process, and particularly relates to the technical field of fruit and vegetable flavored beverage preparation, and the process comprises the following steps: S1, raw material compatibility, S2, double-bacterium inoculation, S3, pulse type ventilation fermentation, S4, intelligent compounding, and S5, post-treatment. According to the preparation method disclosed by the invention, various fruit and vegetable raw materials such as mulberries and pomegranates are scientifically proportioned, and the prepared fruit and vegetable flavor beverage is excellent in indexes such as viable count, ethanol content, precipitation rate, anthocyanin and characteristic flavor substance content and the like through double-bacterium synergistic fermentation, pulse type ventilation four-stage precise temperature control ventilation, intelligent dynamic compounding and a specific post-treatment process, and is high in sensory score and high in quality. Compared with a contrast product in which a strain inoculation mode is changed, key steps are omitted or fermentation parameters are adjusted, the quality is remarkably improved.

The present application relates to the field of microbial technology, and particularly relates to a salt-tolerant and heat-tolerant Myceliophthora strain and application thereof, the strain is Myceliophthora thermophila MT-ST / GP, which has been preserved in CGMCC on October 30, 2024, and the preservation number is CGMCC No.41587. The strain is outstanding in salt tolerance, and when NaCl≤10 g / L, sodium bicarbonate≤5 g / L or 30% NaCl-70% sodium bicarbonate mixed salt≤5 g / L, the average viable cell count is≥20.3, and the strain can grow stably. The strain can be used for straw field return in saline-alkali land, fermentation of high-salt agricultural waste and degradation of high-salt wastewater, and can also be used for efficient fermentation and degradation of wheat straw.

The present invention relates to a method for in-situ microbial remediation or management of petroleum-contaminated sites using micro-nano aeration. The method comprises the following steps: 1) injecting micro-nano bubbles into soil or groundwater for continuous aeration for 3-5 days, with a daily aeration time of 6-10 hours; the air source of the micro-nano bubbles is one or a combination of air and oxygen; and 2) injecting a composite microbial inoculum into the soil or groundwater 12-24 hours after step 1), wherein the composite microbial inoculum comprises composite microorganisms, including Pseudomonas, Reysella, Phenylobacter, and Bacillus brevis, with a viable cell count ratio of 1-25:1-15:1 to 5:1. This method has low energy consumption, does not produce secondary pollution, and has excellent remediation or management effects. After the remediation, the concentration of total petroleum hydrocarbon (C10-C40) pollutants in the contaminated site is significantly reduced.

This invention discloses a strain of *Bifidobacterium longum* SRU-1, possessing both bile salt hydrolase activity and α-glucosidase inhibitory activity, with accession number CGMCC No. 36397. This strain was isolated from infant feces and identified as *Bifidobacterium longum* through morphological and molecular biological analysis. It exhibits excellent acid and bile salt tolerance, is non-hemolytic, and demonstrates superior self-aggregation and hydrophobicity. It can scavenge DPPH free radicals and possesses potential for lowering cholesterol and assisting in blood sugar control. This invention also provides its culture method; optimizing the culture medium can significantly increase the bacterial density, with the viable cell count reaching 1.34 × 10⁻⁶ when 20% prickly pear juice is added. 11 The concentration of CFU / mL is 5.8 times that of TPY medium. This strain can be used to ferment yogurt, producing a product with an acidity of 100°T-110°T and pH 4.4, excellent water retention, and combining the functions of probiotics with the nutritional advantages of prickly pear. It fills the technological gap in prickly pear multifunctional yogurt and is suitable for industrial food production.

This invention relates to a high-density, high-activity mixed-culture fermentation method, its products, and applications. The method includes: inoculating a seed culture of a mixed strain of different strains of lactic acid bacteria into a fermentation medium, and fermenting to obtain the product. The mixed strains of different strains of lactic acid bacteria include a mixed strain of different strains of Streptococcus thermophilus, a mixed strain of different strains of Lactobacillus delbrueckii subsp. bulgaricus, or a mixed strain of different strains of Lactobacillus acidophilus. The fermentation method of this invention improves production efficiency and reduces costs while increasing the yield and viable cell count of the bacterial powder, and the fermentation activity of the obtained bacterial powder is stronger than that of single-strain fermentation.

This invention belongs to the field of plant protein modification and processing technology, specifically relating to a set-type pure walnut-based plant-based yogurt and its preparation method. The yogurt uses peeled walnut kernels as the main raw material, and is produced through protein glutaminase enzymatic hydrolysis, enzyme inactivation, centrifugation, homogenization, and sterilization, followed by inoculation with a commercial starter culture, fermentation, and cold-curing. The method of this invention uses protein glutaminase to treat walnut milk, effectively improving the solubility of walnut protein, significantly improving the water-holding capacity and whey separation rate of the walnut yogurt, and optimizing its texture. Through screening different commercial starter cultures, a suitable starter culture for preparing walnut yogurt was determined, increasing the acidity and viable cell count of the walnut yogurt.

The present invention discloses a compost-promoting, phosphorus-dissolving, and deodorizing bacterial agent, as well as its preparation method and application, belonging to the technical field of bio-agriculture and related industries. The bacterial agent is composed of the following strains in a mass ratio: Bacillus licheniformis DY-1: Paenibacillus zeae C-A: Bacillus chestnutii SC = 1.5:(1-3):(1.5-4); the viable cell count of each strain is ≥5×10 8 CFU / g; the total viable count of the bacterial agent is ≥1×10 9 CFU / g. Its preparation method includes the steps of (1) strain activation, (2) strain expansion culture, (3) bacterial powder preparation, and (4) strain compounding. Its application in clean composting of organic waste. The present invention has the following advantages: not only do the three bacterial strains in the microbial agent not produce antagonism, but they can also significantly accelerate the conversion of insoluble phosphorus, promote decomposition, and enhance the removal of NH3 and H2S.

The invention belongs to the technical field of veterinary biology, and discloses an escherichia coli NK2 strain and application thereof in preparation of a mycoplasma hyopneumoniae culture medium. According to the invention, an Escherichia coli NK2 is screened, and the preservation number of the Escherichia coli NK2 is CCTCC (China Center For Type Culture Collection) NO: M20252722. The mycoplasma hyopneumoniae high-efficiency culture medium is prepared by the applicant by taking fermentation supernate of an endotoxin-removed escherichia coli NK2 strain as an auxiliary culture medium, the growth speed of the mycoplasma hyopneumoniae can be increased by adding the auxiliary culture medium, the culture time of the mycoplasma hyopneumoniae can be shortened, and the yield of the mycoplasma hyopneumoniae can be increased. Therefore, generation of aged bacteria is reduced, the viable count during culture harvesting is remarkably increased, the thallus antigen content during culture harvesting is remarkably improved, and the method has the advantages of being convenient to use, easy to obtain, low in cost, high in economic benefit and the like.

To provide a technique capable of suppressing reduction in viable cell count of Bifidobacterium bacteria in a fermented composition containing whey protein and the Bifidobacterium bacteria.SOLUTION: A fermented composition comprising the following components (A) to (C): (A) a whey protein, (B) a bacterium of the genus Bifidobacterium, and (C) an ash content of 0.05% by mass or more. A method for producing a fermented composition containing the components (A) to (C), the method including a step of blending the component (C) ash. A survival rate-improving agent for improving a survival rate of a bacterium of the genus Bifidobacterium contained in a fermented composition containing a whey protein and the bacterium of the genus Bifidobacterium, wherein the agent contains an ash component as an active ingredient.SELECTED DRAWING: None