9 results about "Viable Cell Count" patented technology

This invention belongs to the field of microbial fermentation technology, specifically relating to a method for preparing microbial inoculants through mixed fermentation of Bacillus subtilis and Bacillus cereus. The method includes: separately activating Bacillus subtilis and Bacillus cereus to obtain a seed culture; adding a basic fermentation medium to a fermenter, first inoculating Bacillus subtilis, and then inoculating Bacillus cereus after it enters the mid-logarithmic growth phase, achieving sequential mixed inoculation; employing segmented temperature control, two-stage pH control, and dynamic sugar feeding during fermentation, while simultaneously adding malic acid in a coupled manner between 20 and 35 hours to alleviate oxidative stress; collecting the cells after fermentation, adding a protective agent, and drying to obtain the inoculant. This invention, through the synergistic effect of sequential inoculation and malic acid coupled feeding, achieves an effective viable cell count of up to 1.0 × 10⁻⁶. 13 With a CFU / mL or higher, the spore formation rate is ≥89%, which is significantly better than the existing mixed-strain fermentation process.

The application discloses an application of astragalus polysaccharide in promoting lactic acid bacteria metabolism and improving quality and stability of fermented products, and belongs to the technical field of fermented dairy product processing. The application adds 2 ‰ to 10 ‰ of astragalus polysaccharide and sucrose in skimmed milk with respect to the mass of the skimmed milk, inoculates lactic acid bacteria starter after pasteurization, and constant-temperature fermentation is carried out until the end point to prepare fermented products. The application utilizes the dual functions of astragalus polysaccharide in promoting lactic acid bacteria metabolism and strengthening milk protein gel network to realize the synergistic improvement of quality and stability of fermented products. Experiments show that when the addition amount of astragalus polysaccharide is 4 ‰, the sensory score of the fermented product reaches 87.3, and the water holding capacity is increased by 11.95 % compared with the control group; scanning electron microscopy shows that the gel network is dense and has few pores; when the addition amount is 6 ‰, the viable cell count reaches 1.23 x 10 8 CFU / ml; and when the addition amount is 8 ‰, the texture characteristics are optimal. The application is simple in operation, suitable for industrial production, and provides a new way for the development of functional fermented dairy products.

The present disclosure relates to: a culture medium composition for culturing lactic acid bacteria, which comprises superfine red pepper powder as an active ingredient; a method for culturing lactic acid bacteria using the culture medium composition for culturing lactic acid bacteria; a method for increasing a viable cell count of lactic acid bacteria; a lactic acid bacteria fermentation broth prepared using the culture method; a probiotic food composition comprising the lactic acid bacteria fermentation broth; and a probiotic beverage composition comprising the lactic acid bacteria fermentation broth.

The present application relates to the field of microbial technology, and particularly relates to a salt-tolerant and heat-tolerant Myceliophthora strain and application thereof, the strain is Myceliophthora thermophila MT-ST / GP, which has been preserved in CGMCC on October 30, 2024, and the preservation number is CGMCC No.41587. The strain is outstanding in salt tolerance, and when NaCl≤10 g / L, sodium bicarbonate≤5 g / L or 30% NaCl-70% sodium bicarbonate mixed salt≤5 g / L, the average viable cell count is≥20.3, and the strain can grow stably. The strain can be used for straw field return in saline-alkali land, fermentation of high-salt agricultural waste and degradation of high-salt wastewater, and can also be used for efficient fermentation and degradation of wheat straw.

This invention discloses a strain of *Bifidobacterium longum* SRU-1, possessing both bile salt hydrolase activity and α-glucosidase inhibitory activity, with accession number CGMCC No. 36397. This strain was isolated from infant feces and identified as *Bifidobacterium longum* through morphological and molecular biological analysis. It exhibits excellent acid and bile salt tolerance, is non-hemolytic, and demonstrates superior self-aggregation and hydrophobicity. It can scavenge DPPH free radicals and possesses potential for lowering cholesterol and assisting in blood sugar control. This invention also provides its culture method; optimizing the culture medium can significantly increase the bacterial density, with the viable cell count reaching 1.34 × 10⁻⁶ when 20% prickly pear juice is added. 11 The concentration of CFU / mL is 5.8 times that of TPY medium. This strain can be used to ferment yogurt, producing a product with an acidity of 100°T-110°T and pH 4.4, excellent water retention, and combining the functions of probiotics with the nutritional advantages of prickly pear. It fills the technological gap in prickly pear multifunctional yogurt and is suitable for industrial food production.

The present application relates to the technical field of soil remediation, and particularly relates to a biological composite soil remediation agent based on marine waste and a preparation method thereof.The soil remediation agent of the present application is composed of shell powder, seaweed extract and microbial inoculum, wherein the viable cell count of the microbial inoculum in the biological composite soil remediation agent based on marine waste is 1-5x10 8 CFU / g; the mass ratio of the shell powder and the seaweed extract is 1-10:0.1-1.The soil remediation agent provided by the present application is expected to achieve more significant and more durable remediation effect on acidic and severely cadmium-contaminated soil in a shorter time, with lower application amount and economic cost, and simultaneously achieve improvement of soil ecological function, thereby providing a brand-new and comprehensive solution for safe utilization of agricultural land.

This invention discloses a high-purity, multifunctional probiotic powder and its preparation method. Based on the different characteristics of lactic acid bacteria, Bacillus subtilis, and actinomycetes, this invention employs different methods for their respective proliferation. This not only avoids potential mutual interference and contamination during their individual proliferation processes but also achieves high viable cell counts while maintaining high purity, and the high viable cell count is maintained even after mixing. This invention mixes the proliferated bacterial powders in a reasonable ratio, allowing lactic acid bacteria to be the dominant strain while fully utilizing the functional characteristics of each of the three bacteria: lactic acid bacteria are anaerobic, while Bacillus subtilis and actinomycetes are aerobic. The latter can absorb oxygen released by the former, ensuring that in the anaerobic environment of the human intestine, Bacillus subtilis and actinomycetes remain active rather than dormant, generating resistant endospores through synergistic action with lactic acid bacteria.

This invention provides a microbial remediation agent for arid and semi-arid regions, its preparation method, and its application, belonging to the field of microbial fertilizers and soil improvement technology. The microbial remediation agent comprises a compound bacterial agent, modified biochar, and adjuvants; the compound bacterial agent is a mixture of Bacillus subtilis, Bacillus licheniformis, and Pseudomonas fluorescens; the modified biochar is prepared by mixing corn straw biochar and diatomaceous earth, then treating it with ultrasound in an aqueous solution of citric acid and fulvic acid. The preparation method of the above-mentioned microbial remediation agent includes: high-density fermentation of the three bacteria separately, determining the viable cell count in the fermentation broth, mixing them in a certain proportion, centrifuging to obtain a compound bacterial precipitate, mixing it with adjuvants, and then loading it onto the modified biochar through low-temperature vacuum adsorption. The microbial remediation agent of this invention can significantly improve microbial survival rate, enhance soil enzyme activity, and promote crop growth under drought stress, making it suitable for soil improvement in arid and semi-arid regions.

The present application relates to the technical field of microorganism and fermentation engineering, and particularly relates to a high-density fermentation method of lactobacillus fermentum. Seed liquid of lactobacillus fermentum HEW-A846 is inoculated into a fermentation medium containing a composite nitrogen source, a UiO-66-NH2 type MOF material is added in stages, and sugar feeding is performed based on a pH and DO double-signal coordinated triggering feeding strategy. The present application realizes high-density fermentation of lactobacillus fermentum HEW-A846 by in-situ selective adsorption of lactic acid by MOF to release product feedback inhibition, combined with precise feeding control. Under optimal process conditions, the viable cell count in a 5L fermenter can reach 3.28x10 10 CFU / mL, the lactic acid accumulation amount is reduced to 11.5 g / L, the process is scaled up to 20m 3 L, the scale-up deviation is less than 10%, and the process has good industrial application prospect.