35 results about "Viable Cell Count" patented technology

This invention discloses a multifunctional Pediococcus pentosaccharide strain and its applications. Pediococcus pentosaceus The strain, named *Pediococcus pentosaceus* A07, has the accession number CCTCC NO: M 2025777 and is deposited at the China Center for Type Culture Collection (CCTCC), located at Wuhan University, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province. This strain is capable of degrading nitrite. Using *Pediococcus pentosaceus* A07 to reduce nitrite content can produce high-quality pickles with good flavor and texture, low nitrite content, and high viable cell count. The *Pediococcus pentosaceus* A07 provided by this invention also possesses cholesterol-lowering, zinc protoporphyrin-forming, antibacterial, and antioxidant capabilities, and produces a high biofilm.

The invention discloses a multi-strain active lactobacillus beverage and a preparation method thereof. The multi-strain active lactobacillus beverage disclosed by the invention is good in flavor and mouth feel, fresh and cool in mouth feel, sour, sweet and delicious, and capable of maintaining the constant viable count within the shelf life and still maintaining the viable count gt after being refrigerated at 2-10 DEG C for 30 days; the method has the advantages of high purity, high purity, 1 * 10 < 9 > CFU / ml, good stability, no layering and precipitation phenomena, and centrifugal precipitation rate maintained within 2%.

This invention belongs to the field of microbial fermentation technology, specifically relating to a method for preparing microbial inoculants through mixed fermentation of Bacillus subtilis and Bacillus cereus. The method includes: separately activating Bacillus subtilis and Bacillus cereus to obtain a seed culture; adding a basic fermentation medium to a fermenter, first inoculating Bacillus subtilis, and then inoculating Bacillus cereus after it enters the mid-logarithmic growth phase, achieving sequential mixed inoculation; employing segmented temperature control, two-stage pH control, and dynamic sugar feeding during fermentation, while simultaneously adding malic acid in a coupled manner between 20 and 35 hours to alleviate oxidative stress; collecting the cells after fermentation, adding a protective agent, and drying to obtain the inoculant. This invention, through the synergistic effect of sequential inoculation and malic acid coupled feeding, achieves an effective viable cell count of up to 1.0 × 10⁻⁶. 13 With a CFU / mL or higher, the spore formation rate is ≥89%, which is significantly better than the existing mixed-strain fermentation process.

The invention discloses a composite flora and activated mineral synergistic bio-organic fertilizer and a preparation method thereof. The preparation method comprises the following steps: crushing and mixing organic raw materials, adding a compound bacterium enzyme preparation, and sequentially carrying out two-stage temperature-controlled fermentation of high-temperature aerobic fermentation and medium-temperature curing to obtain a high-activity fermented material; the preparation method comprises the following steps: carrying out nano micropore activation treatment on monoclinoptilolite by using a hydrochloric acid solution to obtain activated zeolite with high specific surface area; and finally, mixing the fermented material with the activated zeolite in proportion, chelating at low temperature, granulating and drying to obtain the bio-organic fertilizer. Through composite flora construction, mineral nano activation and a step-by-step synergistic process, the effective viable count of the product reaches 2.0 billion CFU / g or above, the Pb < 2 + > adsorption rate exceeds 85%, and nutrient slow release and remarkable growth promoting effects are achieved. The product integrates the functions of soil improvement, disease inhibition, heavy metal restoration and nutrient slow release, and is suitable for ecological planting of various crops.

To provide a method for appropriately setting the intensity of ultraviolet rays at a flow rate at which optimum power efficiency is obtained in order to prevent infection to individuals in other water tanks even if an individual in one of a plurality of water tanks develops an infectious disease in a water circulation system.SOLUTION: The management device 100 is configured to, based on a sterilization target value including a safe maximum viable cell count and an allowable immersion time, bacteria information including a viable cell count increase amount indicating the number of bacteria released into water per unit time by the aquatic organisms having developed infectious diseases and ultraviolet light sensitivity information of bacteria to be sterilized, and rearing condition information including a plurality of water tank 20A, a water amount of 20B, the number of water tanks, and a lower limit flow amount of a pump, A specifying unit configured to set an ultraviolet target intensity of the ultraviolet light source so that the number of viable bacteria in at least one of the plurality of water tanks falls below the safe maximum number of viable bacteria within the allowable immersion time when the set flow rate of the pump is set to a target flow rate based on a lower limit flow rate.SELECTED DRAWING: Figure 1

The invention provides bacillus velezensis and application thereof, and belongs to the technical field of microorganisms. The bacillus velezensis ND-J with a remarkable inhibition effect on main pathogenic bacteria of the ginger root rot is successfully obtained, and the fermentation viable count of the bacillus velezensis ND-J is greatly increased through system optimization, so that a reliable strain resource and a mature fermentation process foundation are provided for developing an efficient and stable ginger root rot biological control preparation. The application of the technology is helpful to reduce the use of chemical pesticides, promotes the green and sustainable development of the ginger industry, and has positive significance in guaranteeing the yield and quality of ginger and increasing the economic benefits of ginger farmers.

The application discloses an application of astragalus polysaccharide in promoting lactic acid bacteria metabolism and improving quality and stability of fermented products, and belongs to the technical field of fermented dairy product processing. The application adds 2 ‰ to 10 ‰ of astragalus polysaccharide and sucrose in skimmed milk with respect to the mass of the skimmed milk, inoculates lactic acid bacteria starter after pasteurization, and constant-temperature fermentation is carried out until the end point to prepare fermented products. The application utilizes the dual functions of astragalus polysaccharide in promoting lactic acid bacteria metabolism and strengthening milk protein gel network to realize the synergistic improvement of quality and stability of fermented products. Experiments show that when the addition amount of astragalus polysaccharide is 4 ‰, the sensory score of the fermented product reaches 87.3, and the water holding capacity is increased by 11.95 % compared with the control group; scanning electron microscopy shows that the gel network is dense and has few pores; when the addition amount is 6 ‰, the viable cell count reaches 1.23 x 10 8 CFU / ml; and when the addition amount is 8 ‰, the texture characteristics are optimal. The application is simple in operation, suitable for industrial production, and provides a new way for the development of functional fermented dairy products.

The present disclosure relates to: a culture medium composition for culturing lactic acid bacteria, which comprises superfine red pepper powder as an active ingredient; a method for culturing lactic acid bacteria using the culture medium composition for culturing lactic acid bacteria; a method for increasing a viable cell count of lactic acid bacteria; a lactic acid bacteria fermentation broth prepared using the culture method; a probiotic food composition comprising the lactic acid bacteria fermentation broth; and a probiotic beverage composition comprising the lactic acid bacteria fermentation broth.

Disclosed herein are impedance-based biosensor systems comprising an impedance-based biosensor having an electrode, a meter in electrical communication with the electrode, a processor in electrical communication with the impedance-based biosensor and the meter, and a memory storing instructions to be executed by the processor. The instructions can cause the impedance-based biosensor system to measure the impedance of the electrode, derive one or more electrical properties of the impedance-based biosensor from the impedance of the electrode, and calculate a viable cell count based on the one or more electrical properties.

The invention discloses bacillus licheniformis YHD2 and application thereof in degrading nitrite and inhibiting aquatic pathogenic bacteria, and belongs to the technical field of microorganisms. The preservation number of the bacillus licheniformis YHD2 is CCTCC NO: M 20251259, and the bacterial colony of the bacillus licheniformis YHD2 is milk white, opaque, irregular in edge and rough in surface; the viable count of the prepared bacterial powder is at least 1 * 10 < 9 > CFU / g, and the bacillus licheniformis YHD2 has the capability of rapidly degrading nitrite and the capability of inhibiting aquatic pathogenic bacteria. Experiments prove that the novel bacillus licheniformis YHD2 has important significance on development of aquaculture water quality purification and preparation of a bacteriostatic agent for aquatic pathogenic bacteria, the application range of the bacillus licheniformis YHD2 is further widened, and the bacillus licheniformis YHD2 has wide economic value and development prospects in aquaculture.

The invention discloses a fermented feed additive for replacing soybean meal as well as a preparation method and application of the fermented feed additive. The fermented feed additive disclosed by the invention is prepared by performing acid protease enzymolysis on a mixed fermentation raw material and fermenting the mixed fermentation raw material with a complex microbial inoculant, wherein the mixed fermentation raw materials comprise the following components in parts by weight: 40-80 parts of corn protein powder, 30-60 parts of corn steep liquor, 7-12 parts of corn germ meal, 4-15 parts of molasses and 2-5 parts of extruded soybeans; the complex microbial inoculant is composed of pediococcus acidilactici, candida utilis and bacillus subtilis according to the viable count ratio of (1-3): (1-3): (0.1-1). The fermented feed additive can effectively replace soybean meal in animal daily ration, the utilization rate of nutrients by animals is increased, the health level of the animals is improved, and the production performance of the animals is improved.

The invention provides heat-resistant pediococcus acidilactici and application of the heat-resistant pediococcus acidilactici in pellet feed production. The heat-resistant pediococcus acidilactici is named as pediococcus acidilactici YJ and is preserved in the China Center for Type Culture Collection on May 19, 2025, and the preservation number of the heat-resistant pediococcus acidilactici is CCTCC (China Center for Type Culture Collection) NO: M 20251096. The strain YJ has heat-resistant and extrusion-resistant functions, and can be added into a feed production process before particle forming to obtain a feed product with high viable count and uniform addition, so that the growth and intestinal development of chicks are promoted, intestinal injury is repaired, and the production performance of the chicks is improved. The strain YJ can tolerate the high temperature of 90 DEG C, and can be directly added in a mixing stage, so that the strain YJ can be uniformly added in powdery, hard pellet feed and expanded pellet feed, meanwhile, the magnitude order of viable count can reach 107 cfu / g feed, and meanwhile, the variable coefficient of mixing uniformity is less than 5.0%.

The application provides a grape juice enzyme fermentation method and application, and relates to the technical field of fermentation.The grape juice enzyme fermentation method provided by the application comprises the following steps: inoculating fermentation bacteria in a culture medium containing grape juice to perform fermentation, and preparing grape juice enzyme; the fermentation bacteria comprise lactobacillus plantarum Zhang-LL and kloeckera apiculata M3; the preservation number of the lactobacillus plantarum Zhang-LL is CGMCC No.6936; and the preservation number of the kloeckera apiculata M3 is CGMCC No.1811.The fermentation method uses the lactobacillus plantarum Zhang-LL and the kloeckera apiculata M3 fermentation bacteria, and through the synergistic cooperation of the two, the fermentation time is shortened, and a high viable cell count can be achieved; the grape juice enzyme can be significantly improved in terms of antioxidant property and total protease activity; and the application provides a theoretical basis for industrialized preparation of grape enzyme and subsequent research and development of multifunctional products.

This invention discloses a high SOD activity *Gnaphalium affine* juice, a *Gnaphalium affine* juice probiotic fermented product, and its preparation method. Using *Gnaphalium affine* as raw material, this invention obtains a high SOD activity *Gnaphalium affine* juice through a combination of repeated low-temperature treatment, pulping, ultrasonic crushing pretreatment, and heat treatment. Fermentation is carried out using *Lactobacillus mucilaginosus* Grx07, with the addition of a commercial fermenting agent to regulate the fermentation process. This achieves a synergistic effect between probiotics and *Gnaphalium affine* juice in terms of SOD activity, improving the SOD activity, viable cell count, and sensory quality of the fermented product. It has broad application prospects in the preparation of probiotic fermented foods with high antioxidant activity.

The invention relates to the technical field of soil improvement. The invention provides a saline-alkali soil improving microbial agent and a preparation method thereof. The saline-alkali soil improving microbial agent is prepared from the following raw materials in parts by weight: 15 to 20 parts of bacillus megatherium, 10 to 15 parts of bacillus subtilis, 8 to 12 parts of bacillus mucilaginosus, 5 to 8 parts of pseudomonas fluorescens, 6 to 10 parts of trichoderma harzianum, 5 to 8 parts of arbuscular mycorrhizal fungi and 3 to 5 parts of rhizobium. Through cooperative use of the strains, the salt content of the coastal salinized soil can be effectively reduced, the pH value can be adjusted, the nutrient index of the salinized soil can be improved, the content of microorganisms in the soil can be improved, and the root development of crops can be promoted. Meanwhile, the sectional fermentation process can solve the problem of strain antagonism, and the salt tolerance and viable count of the strain are improved in cooperation with a special composite culture medium, so that the saline-alkali soil improvement effect is further enhanced.

The application discloses application of Pseudomonas pseudoalcaligenes 94-6Y in removing pollutants in wastewater, and belongs to the technical field of microorganisms. The Pseudomonas pseudoalcaligenes 94-6Y has the preservation number of CGMCC No.32718, has the characteristic of tolerating penicillin, can grow in penicillin wastewater with the concentration of 0.1-2.5 mg / L, and the effective viable cell count can reach 5x10 8 CFU / mL after 72 hours in the penicillin-containing wastewater, and it is also proved that the Pseudomonas pseudoalcaligenes 94-6Y has the characteristic of efficiently reducing pollutants in wastewater, wherein the removal rate of chemical oxygen demand reaches 68.41%, the removal rate of ammonia nitrogen reaches 85.92%, and the removal rate of total phosphorus reaches 21.3%, which has a significant effect on improving the water quality of tail water of antibiotic production industry and reaching the discharge standard.

The present application relates to the field of microbial technology, and particularly relates to a salt-tolerant and heat-tolerant Myceliophthora strain and application thereof, the strain is Myceliophthora thermophila MT-ST / GP, which has been preserved in CGMCC on October 30, 2024, and the preservation number is CGMCC No.41587. The strain is outstanding in salt tolerance, and when NaCl≤10 g / L, sodium bicarbonate≤5 g / L or 30% NaCl-70% sodium bicarbonate mixed salt≤5 g / L, the average viable cell count is≥20.3, and the strain can grow stably. The strain can be used for straw field return in saline-alkali land, fermentation of high-salt agricultural waste and degradation of high-salt wastewater, and can also be used for efficient fermentation and degradation of wheat straw.

This invention discloses a strain of *Bifidobacterium longum* SRU-1, possessing both bile salt hydrolase activity and α-glucosidase inhibitory activity, with accession number CGMCC No. 36397. This strain was isolated from infant feces and identified as *Bifidobacterium longum* through morphological and molecular biological analysis. It exhibits excellent acid and bile salt tolerance, is non-hemolytic, and demonstrates superior self-aggregation and hydrophobicity. It can scavenge DPPH free radicals and possesses potential for lowering cholesterol and assisting in blood sugar control. This invention also provides its culture method; optimizing the culture medium can significantly increase the bacterial density, with the viable cell count reaching 1.34 × 10⁻⁶ when 20% prickly pear juice is added. 11 The concentration of CFU / mL is 5.8 times that of TPY medium. This strain can be used to ferment yogurt, producing a product with an acidity of 100°T-110°T and pH 4.4, excellent water retention, and combining the functions of probiotics with the nutritional advantages of prickly pear. It fills the technological gap in prickly pear multifunctional yogurt and is suitable for industrial food production.

This invention relates to a high-density, high-activity mixed-culture fermentation method, its products, and applications. The method includes: inoculating a seed culture of a mixed strain of different strains of lactic acid bacteria into a fermentation medium, and fermenting to obtain the product. The mixed strains of different strains of lactic acid bacteria include a mixed strain of different strains of Streptococcus thermophilus, a mixed strain of different strains of Lactobacillus delbrueckii subsp. bulgaricus, or a mixed strain of different strains of Lactobacillus acidophilus. The fermentation method of this invention improves production efficiency and reduces costs while increasing the yield and viable cell count of the bacterial powder, and the fermentation activity of the obtained bacterial powder is stronger than that of single-strain fermentation.

This invention belongs to the field of plant protein modification and processing technology, specifically relating to a set-type pure walnut-based plant-based yogurt and its preparation method. The yogurt uses peeled walnut kernels as the main raw material, and is produced through protein glutaminase enzymatic hydrolysis, enzyme inactivation, centrifugation, homogenization, and sterilization, followed by inoculation with a commercial starter culture, fermentation, and cold-curing. The method of this invention uses protein glutaminase to treat walnut milk, effectively improving the solubility of walnut protein, significantly improving the water-holding capacity and whey separation rate of the walnut yogurt, and optimizing its texture. Through screening different commercial starter cultures, a suitable starter culture for preparing walnut yogurt was determined, increasing the acidity and viable cell count of the walnut yogurt.

The invention belongs to the technical field of veterinary biology, and discloses an escherichia coli NK2 strain and application thereof in preparation of a mycoplasma hyopneumoniae culture medium. According to the invention, an Escherichia coli NK2 is screened, and the preservation number of the Escherichia coli NK2 is CCTCC (China Center For Type Culture Collection) NO: M20252722. The mycoplasma hyopneumoniae high-efficiency culture medium is prepared by the applicant by taking fermentation supernate of an endotoxin-removed escherichia coli NK2 strain as an auxiliary culture medium, the growth speed of the mycoplasma hyopneumoniae can be increased by adding the auxiliary culture medium, the culture time of the mycoplasma hyopneumoniae can be shortened, and the yield of the mycoplasma hyopneumoniae can be increased. Therefore, generation of aged bacteria is reduced, the viable count during culture harvesting is remarkably increased, the thallus antigen content during culture harvesting is remarkably improved, and the method has the advantages of being convenient to use, easy to obtain, low in cost, high in economic benefit and the like.

To provide a technique capable of suppressing reduction in viable cell count of Bifidobacterium bacteria in a fermented composition containing whey protein and the Bifidobacterium bacteria.SOLUTION: A fermented composition comprising the following components (A) to (C): (A) a whey protein, (B) a bacterium of the genus Bifidobacterium, and (C) an ash content of 0.05% by mass or more. A method for producing a fermented composition containing the components (A) to (C), the method including a step of blending the component (C) ash. A survival rate-improving agent for improving a survival rate of a bacterium of the genus Bifidobacterium contained in a fermented composition containing a whey protein and the bacterium of the genus Bifidobacterium, wherein the agent contains an ash component as an active ingredient.SELECTED DRAWING: None

The present application relates to the technical field of soil remediation, and particularly relates to a biological composite soil remediation agent based on marine waste and a preparation method thereof.The soil remediation agent of the present application is composed of shell powder, seaweed extract and microbial inoculum, wherein the viable cell count of the microbial inoculum in the biological composite soil remediation agent based on marine waste is 1-5x10 8 CFU / g; the mass ratio of the shell powder and the seaweed extract is 1-10:0.1-1.The soil remediation agent provided by the present application is expected to achieve more significant and more durable remediation effect on acidic and severely cadmium-contaminated soil in a shorter time, with lower application amount and economic cost, and simultaneously achieve improvement of soil ecological function, thereby providing a brand-new and comprehensive solution for safe utilization of agricultural land.

The invention relates to the technical field of food microorganisms, in particular to a high-activity probiotic double-layer tablet with a decayed tooth resisting function and a preparation method of the high-activity probiotic double-layer tablet. According to the probiotic double-layer tablet, fructo-oligosaccharide is further compounded on the basis of strain screening and compounding, the strain activity is better maintained, and then the decayed tooth resisting effect is enhanced. On the basis of probiotics and prebiotics, natural plant extracts are scientifically matched, streptococcus mutans are better inhibited, and the decayed tooth resisting effect is further enhanced. Meanwhile, the stability of the probiotic double-layer tablet is inspected, the viable count is more than 1 billion CFU / g at 20 DEG C for 12 months, and the viable survival rate is more than 35%. The obtained probiotic double-layer tablet is high in pressure resistance, good in stability, high in probiotic survival rate, low in product cost and beneficial to large-scale industrial production.

The invention discloses a Mingster small strange bacterium with a potassium dissolving function as well as a fungicide and application of the Mingster small strange bacterium. The preservation number of the Mingster small strange bacterium is CCTCC (China Center For Type Culture Collection) NO: M 20252079. The strain has multiple plant growth promoting functions of dissolving insoluble potassium, dissolving inorganic phosphorus and secreting indoleacetic acid (IAA) and siderophores. The invention also provides a potassium-dissolving bacterial agent prepared from the bacterial strain and a preparation method of the potassium-dissolving bacterial agent, wherein the bacterial agent is preferably a liquid preparation of which the viable count is not less than 1.0 * 10 < 8 > CFU / mL. The microbial inoculum is widely applied in agriculture, and is especially suitable for promoting tomato growth, increasing the potassium content of tomato plants and activating a soil potassium bank. A pot experiment shows that the biomass and potassium absorption of tomatoes can be remarkably improved by applying the microbial inoculum; in plant-free soil, the content of rapidly available potassium in the soil can be increased by 147.8% after the fertilizer is applied for 14 days. The invention provides an efficient microbial fertilizer resource for green agriculture.

The invention provides pediococcus pentosaceus RP-24, application of the pediococcus pentosaceus RP-24 and aquaculture feed. The pediococcus pentosaceus RP-24 is preserved in the China Center for Type Culture Collection on April 8, 2025, and the preservation number of the pediococcus pentosaceus RP-24 is CCTCC M 2025728. The pediococcus pentosaceus RP-24 is derived from a new lactic acid bacterium separated from soy sauce brewed by an ancient method in a Qinanhan vegetable market in Qinzhou City in Guangxi Zhuang Autonomous District, is classified and named as the pediococcus pentosaceus RP-24, has excellent salt resistance and acid resistance, also has strong antioxidant activity and excellent cell surface characteristics, and can be used for preparing soy sauce for a long time, so that the pediococcus pentosaceus RP-24 can be used for preparing soy sauce for a long time. The Pediococcus pentosaceus RP-24 fermented feed has relatively high viable count and thermal stability, and compared with original feed, the fermented feed has relatively high proline and other amino acid molecules capable of improving the salt stress ability of aquatic organisms, which indicates that the Pediococcus pentosaceus RP-24 fermented feed can relieve the salt stress response of aquatic animals and improve the salt stress ability of the aquatic animals. And the damage to aquatic organisms caused by salt stress can be relieved.

This invention discloses a high-purity, multifunctional probiotic powder and its preparation method. Based on the different characteristics of lactic acid bacteria, Bacillus subtilis, and actinomycetes, this invention employs different methods for their respective proliferation. This not only avoids potential mutual interference and contamination during their individual proliferation processes but also achieves high viable cell counts while maintaining high purity, and the high viable cell count is maintained even after mixing. This invention mixes the proliferated bacterial powders in a reasonable ratio, allowing lactic acid bacteria to be the dominant strain while fully utilizing the functional characteristics of each of the three bacteria: lactic acid bacteria are anaerobic, while Bacillus subtilis and actinomycetes are aerobic. The latter can absorb oxygen released by the former, ensuring that in the anaerobic environment of the human intestine, Bacillus subtilis and actinomycetes remain active rather than dormant, generating resistant endospores through synergistic action with lactic acid bacteria.

The invention relates to the technical field of microbial fermentation freeze-drying, and discloses a high-density fermentation freeze-drying preparation method of high-activity lactobacillus paracasei KFY202407, which comprises the following steps: carrying out activation culture and amplification culture on the lactobacillus paracasei KFY202407 by using an exclusive culture medium to obtain a seed solution; inoculating the seed solution into a fermentation culture medium for high-density anaerobic fermentation culture to obtain a fermentation solution; and carrying out centrifugal enrichment treatment on the fermentation liquid, then carrying out gradient freeze-drying, and after the freeze-drying is finished, crushing and sieving to obtain the high-activity lactobacillus paracasei KFY202407 high-density fermentation freeze-dried powder. A special fermentation culture medium matched with the strain is adopted, a pure plant source and a multi-stage nitrogen source are compounded, synergistic components such as astragalus polysaccharide and xylooligosaccharide are accurately added, a balanced and lasting nutrient flow matched with the metabolic characteristics of the strain is provided for the strain, a two-stage fermentation process is combined, metabolite inhibition is effectively relieved, and the yield of the strain is increased. Finally, the viable count of the fermentation liquor stably reaches 2.0 * 10 < 10 > CFU / mL or above.

The application belongs to the technical field of microbial fermentation, and particularly relates to a high-density fermentation metabolic regulation method of lactic acid bacteria. The application provides a high-density culture method of lactic acid bacteria. The formula of the high-density fermentation culture medium is as follows: 10-20 g / L of proteose peptone, 10-15 g / L of beef extract, 5-10 g / L of yeast powder, 8-15 g / L of D-sorbitol, 5-10 g / L of L-proline, 3-7 g / L of sodium chloride, 0.3-0.6 g / L of magnesium sulfate 7H2O, 0.1-0.2 g / L of manganese sulfate 1H2O, 0.1-0.2 g / L of ferrous sulfate 7H2O, and pH is 6.0-7.0. The fermentation culture medium significantly improves the viable cell count of Lactobacillus fermentum CQPC04, Lactobacillus rhamnosus 4F225, Lactobacillus plantarum H6 and Lactobacillus gasseri GS18, realizes high-density culture of lactic acid bacteria, and lays a foundation for industrialized production of lactic acid bacteria.

The invention discloses application of pseudobacillus Australii 94-6Y in removal of pollutants in wastewater, and belongs to the technical field of microorganisms. The preservation number of the pseudomonas australis is CGMCC No.32718, the pseudomonas australis 94-6Y has the characteristic of penicillin tolerance and can grow in penicillin wastewater with the concentration of 0.1-2.5 mg / L, the effective viable count of the pseudomonas australis 94-6Y can reach 5 * 10 < 8 > CFU / mL after the pseudomonas australis 94-6Y is put in the penicillin-containing wastewater for 72 hours, and the pseudomonas australis 94-6Y has the characteristic of penicillin tolerance and can grow in the penicillin wastewater with the concentration of 0.1-2.5 mg / L. Meanwhile, it is also proved that the pseudobacillus Australiae 94-6Y has the characteristic of efficiently reducing pollutants in wastewater, the chemical oxygen demand removal rate reaches 68.41%, the ammonia nitrogen removal rate reaches 85.92%, the total phosphorus removal rate reaches 21.3%, and the pseudobacillus Australiae 94-6Y has remarkable effects on improving the quality of tail water of the antibiotic production industry and achieving the emission standard.