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Granular bacillus compound microbial fertilizer as well as preparation method and application thereof

A technology of compound microorganism and bacillus, applied in application, organic fertilizer, fertilizer mixture, etc., can solve problems such as inconvenient mechanized application, and achieve the effect of protecting health, simple preparation method, and stable release of fertilizer effect.

Pending Publication Date: 2019-03-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of these products on the market are powders, and the field effect is good; however, it is not convenient to cooperate with mechanized application in large-scale intensive planting areas such as Northeast China and Xinjiang

Method used

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  • Granular bacillus compound microbial fertilizer as well as preparation method and application thereof
  • Granular bacillus compound microbial fertilizer as well as preparation method and application thereof
  • Granular bacillus compound microbial fertilizer as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 The dynamic change of functional bacteria quantity in the production and preservation process of granular compound microbial fertilizer

[0036] 1. Obtaining granular organic-inorganic compound fertilizer

[0037] Market purchases or self-produced products require total nutrients (N-P 2 o 5 -K 2 O) content ≥ 15%, organic matter content ≥ 20%, moisture content ≤ 5%.

[0038] Such as the granular organic-inorganic compound fertilizer produced by Hubei Yishizhuang Agricultural Technology Co., Ltd.

[0039] 2. Production of Bacillus liquid inoculum

[0040] Inoculate Bacillus amyloliquefaciens SQR9 (Bacillus amyloliquefaciens SQR9) with the preservation number CGMCC NO.5808 into LB culture medium (peptone 10g, yeast powder 5g, NaCl 10g, distilled water 1L, sterilized at 121°C for 20min), carry out liquid fermentation, and ferment The conditions are: the culture temperature is 37°C, the stirring speed is 180 rpm, spores are formed in the middle and late stages o...

Embodiment 2

[0045] Embodiment 2 Greenhouse pot experiment

[0046] 1. Pepper pot experiment

[0047] The pot experiment was carried out in the greenhouse of the College of Resources and Environmental Sciences, Nanjing Agricultural University. The test crop was hot pepper, and the variety was Sujiao No. 5. A total of 5 treatments were set up, namely, no fertilizer (CK), chemical fertilizer (CF), Granular organic-inorganic compound fertilizer (OIF), granular compound microbial fertilizer (CMF) and sterilized granular compound microbial fertilizer (SCMF) were applied. Each treatment includes 5 pots of repetitions, 2 kg of soil per pot, OIF, CMF and SCMF are treated with a fertilizer addition of 0.5% (w / w), and CF is treated with urea, superphosphate and potassium sulfate to make NPK The total nutrients were the same as the above three treatments. Fertilizer and soil are fully mixed and evenly managed. Cultivate seedlings in seedling trays, and after they grow to two leaves and one heart, ...

Embodiment 3

[0055] 1. Obtaining granular organic-inorganic compound fertilizer

[0056] Market purchases or self-produced products require total nutrients (N-P 2 o 5 -K 2 O) content ≥ 15%, organic matter content ≥ 20%, moisture content ≤ 5%.

[0057] Such as the granular organic-inorganic compound fertilizer produced by Hubei Yishizhuang Agricultural Technology Co., Ltd.

[0058] 2. Production of Bacillus liquid inoculum

[0059] Inoculate the Bacillus bacterium NJAU-5 of CGMCC No.12338 into LB culture medium (peptone 10g, yeast powder 5g, NaCl 10g, distilled water 1L, sterilize at 121°C for 20min), and carry out liquid fermentation. The fermentation conditions are: culture temperature 37°C, stirring speed 180 rpm, spores are formed in the middle and late stages of fermentation, so that the amount of bacteria or spores in the fermentation broth is ≥7×10 8 CFU / mL.

[0060] 3. Production of granular compound microbial fertilizer

[0061] The above-mentioned bacillus fermentation liqu...

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Abstract

The invention provides granular bacillus compound microbial fertilizer as well as a preparation method and application thereof. According to the process, bacillus fermentation broth (with abacteria density of more than or equal to 7*108 CFU / g) is fully and evenly mixed with granular organic-inorganic compound fertilizer (with total nutrient content (N-P2O5-K2O) of more than or equal to 15%, organic matter content of more than or equal to 20% and water content of less than or equal to 5%) in a proportion of 3-10% (v / w), and the obtained mixture is air-dried, so that the granular compound microbial fertilizer is prepared. The product has a viable cell count of more than 5*107 CFU / g after being stored for 1 year under normal temperature conditions, and is in line with national standards; theproduct shows a significant growth-promoting effect compared with fertilizer containing the same nutrients in pot experiments of chilies and tomatoes. The novel fertilizer combines the quick-acting effect of chemical fertilizer, the long-acting effect of organic fertilizer and the synergistic function of biological fertilizer, is good in growth-promoting effect, low in production cost and convenient to apply, and can be applied to food crops, vegetable crops and the like.

Description

technical field [0001] The invention belongs to the field of biological fertilizers, and relates to a granular bacillus compound microbial fertilizer and a preparation method and application thereof. Background technique [0002] Since the reform and opening up, the transformation of my country's traditional agriculture into modern efficient and intensive agriculture has provided a guarantee for increasing agricultural production and farmers' income. Among them, the extensive use of chemical fertilizers is an important factor supporting the increase in grain production. my country accounts for 8% of the world's cultivated land. More than 30% of the world's chemical fertilizers. At the same time, the long-term excessive application of chemical fertilizers has also caused many negative effects, specifically manifested as severe damage to the soil structure, a sharp drop in organic matter content, and a serious imbalance in the structure of the soil microbial community, which ev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C05G3/80
CPCC05F11/08C05G3/00C05G3/80C05G1/00
Inventor 沈其荣张楠黄启为
Owner NANJING AGRICULTURAL UNIVERSITY
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