Microbial composite organic fertilizer for preventing and controlling wheat scab, and preparation method thereof
A microbial and organic fertilizer technology, which is applied in the field of microbial compound organic fertilizer for the prevention and treatment of wheat scab and its preparation, can solve the problems of human and livestock harm, affecting wheat quality, affecting wheat yield, etc., to increase disease resistance, improve The effect of plant disease resistance and disease resistance improvement
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Embodiment 1
[0053] A microbial compound organic fertilizer, which is composed of Trichoderma harzianum, Bacillus subtilis, Lactobacillus, and yeast to form a microbial compound agent, plus decomposed straw powder. The specific preparation steps are as follows:
[0054] 1. Preparation of microbial compound bacterial agent:
[0055] Inoculate Trichoderma harzianum on the PDA solid slant medium under aseptic conditions, and cultivate it in an incubator at 30°C for 48 hours. After the green hyphae grow on it, transfer it to PDA liquid medium, and cultivate it at 30°C, 180rpm 72h, then transfer the seed liquid to the fermenter for expansion cultivation, the fermenter volume is 50L, the medium is 30L, the temperature is 30°C, the stirring speed is 180rpm, and the aeration ratio is 0.6vvm. After 72h of fermentation, the fermented liquid Store at low temperature and mix with dried corn flour at a mass ratio of 1:10.
[0056] Inoculate Bacillus subtilis on LB solid medium, culture it in an incuba...
Embodiment 2
[0062] A microbial compound organic fertilizer, which is composed of Trichoderma harzianum, Bacillus subtilis, Lactobacillus, and yeast to form a microbial compound agent, plus decomposed straw powder. The specific preparation steps are as follows:
[0063] 1. Preparation of microbial compound bacterial agent:
[0064] Inoculate Trichoderma harzianum on PDA solid slant medium under aseptic conditions, and culture it in an incubator at 32°C for 46 hours. After the green hyphae grow on it, transfer it to PDA liquid medium, at 32°C, 180rpm Cultivate under conditions for 70 hours, and then transfer the seed liquid to a fermenter for expansion. The volume of the fermenter is 50L, the medium is 30L, and the conditions for expansion: temperature is 32°C, stirring speed is 180rpm, and the aeration ratio is 0.6vvm. After 70 hours of fermentation, the fermented liquid was stored at low temperature and mixed with dried corn flour at a mass ratio of 1:9.
[0065] Inoculate Bacillus subti...
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