14 results about "Gibberella" patented technology

The invention provides a vanillin compound spliced by polyheterocycles. The polyheterocycles are one of a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring; the vanillin compound spliced by polyheterocycles is one of vanillin derivatives containing a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring, and the chemical general formula is shown in the specification. The polyheterocycle spliced vanillin compound is prepared through a condensation reaction and a condensation-cyclization reaction, and the compound has good antibacterial activity on valsa mali, red kidney bean root rot, fusarium graminearum, millet pyricularia grisea, botrytis cinerea, alternaria solani and watermelon fusarium oxysporum. The compound can be used for preparing a novel efficient antibacterial agent and is applied to prevention and treatment of the pathogenic bacteria.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The application provides a streptomyces LZZY-S3 and application thereof, and belongs to the field of microorganisms.The streptomyces LZZY-S3 provided by the application enriches strains for biological control, and realizes the dual functions of plant resistance to pathogenic bacteria and plant promotion.The streptomyces LZZY-S3 has the function of inhibiting fungi in a wide range, and can be applied to plant resistance to pathogenic bacteria, and the pathogenic bacteria include Exobasidium vexans, Botryosphaeria spp., Gibberella, Fusarium oxysporum, Pythium, Fusarium graminearum, Didymella, Fusarium pseudograminearum, Trichothecium roseum, Coniella diplodiella, Colletotrichum graminicola or Rhizoctonia cerealis, wherein the inhibition rate of Exobasidium vexans reaches 91.2%.The streptomyces LZZY-S3 can also be applied to plant promotion, preparation of nitrogen-fixing bacterial agents and preparation of ACC deaminase bacterial agents and the like.

The utility model relates to the technical field of gibberella fermentation, in particular to a waste residue treatment device for gibberella fermentation, which comprises a support table, an outer cylinder is mounted at the top of the support table in a penetrating manner, a cover plate is placed at the top of the outer cylinder, a support frame is connected to the center of the top of the cover plate, and a variable frequency motor is mounted on the support frame in a penetrating manner. The output end of the variable frequency motor and the interior of the outer cylinder are jointly connected with a processing part. According to the utility model, the variable frequency motor runs slowly, and meanwhile, the telescopic end of the electric telescopic rod II extends out to further open the electromagnetic valve on the discharge pipe; when the telescopic end of the second electric telescopic rod corresponds to the arc-shaped notch, the follow-up variable frequency motor runs at a high speed, so that liquid in the gibberella fermentation waste residues is thrown out and discharged from the discharging pipe. The gibberellic acid fermentation waste residues are crushed by the main heating crushing rod and the auxiliary heating crushing rod, and the gibberellic acid fermentation waste residues are dried by the heating column, the main heating crushing rod and the auxiliary heating crushing rod.

The invention provides genetic engineering construction and application of a gibberellin GA4 + 7 efficient production strain. According to the method, the speed limiting step of a metabolic pathway of gibberella zeylanica is explored, key genes fpps, gs2, cps / ks and thmgr of the gibberella zeylanica are subjected to editing expression, the screened key genes ggs2, cps / ks and thmgr are subjected to co-expression, screening markers are recycled by using an inducible recombinase-based gibberella zeylanica screening marker recycling system, and finally, the strain OE: thmgr-gs2-cps / ks with the highest yield is obtained. The yield of GA4 + 7 in an optimized fermentation culture medium reaches 3.6 g / L.

The invention belongs to the technical field of crop variety innovation, and particularly relates to a breeding method and application of wheat capable of resisting gibberellic disease and herbicide-tolerant starter propagation. According to the method disclosed by the invention, through combination of molecular marker-assisted selection and backcross breeding, excellent characters from different donors are rapidly introduced into a genetic background of an excellent koji-making variety, so that accurate and efficient polymerization of target characters is realized, and the screening efficiency and accuracy can be improved; meanwhile, the screening index of starter propagation quality runs through the breeding process, so that the bred material is not only excellent in resistance and agronomic character, but also can meet the specific requirements of the terminal industry. The variety bred by the method provided by the invention can reduce the use of pesticides for preventing and treating gibberellic disease and reduce the pollution risk of fungaltoxin; the herbicide-resistant characteristic facilitates field weed management, and the yield and quality stability are improved; the special quality guarantees the high additional value in the starter propagation industry, and creates the value of the whole industrial chain from the field to the workshop.

The invention relates to the technical field of microorganisms and application thereof, and discloses a piggyBac transposon system, a recombinant vector, a gene editing method, a recombinant strain and application of the piggyBac transposon system, the piggyBac transposon system contains piggyBac transposase, an inverted terminal repeat sequence, a target load gene sequence and a selective marker gene, the vector gene is a functional marker gene and / or a reporter gene, and the expression of the transposase is controlled through a promoter PtrpC; wherein the amino acid sequence of the piggyBac transposase is as shown in SEQ ID NO. 1; and the piggyBac transposase system also contains a vector plasmid. The gene editing method of the piggyBac transposon system can be suitable for efficiently and accurately inserting exogenous genes into gibberella zeylanica, traceless and residue-free genome modification is realized, the problem of exogenous gene residue is avoided, and the method has relatively good industrial value.

This invention discloses a molecular breeding method, molecular markers, and primers for selecting varieties highly resistant to Fusarium head blight. The molecular markers include those with flanking sequences as shown in SEQ ID NO.3. TaFhb‑2DL The KASP marker. This invention utilizes fine-mapping populations to narrow down Fusarium head blight resistance sites. QFhb-2DL By analyzing the physical regions of the genome, candidate genes were identified, and the genes most associated with Fusarium head blight resistance were screened using the phenotypes and genotypes of natural populations. A new linkage marker, KASP.TaFhb-2DL, was developed to enable... QFhb-2DL The utilization is made more efficient. This invention utilizes genes carrying resistance to Fusarium head blight. TaFhb‑ 2DL Varieties with disease-resistant genotypes, used as parents, carry... Fhb1 Gene varieties serve as donors, because TaFhb‑2DL It has been effectively utilized in wheat scab resistance breeding in the middle and lower reaches of the Yangtze River, and on this basis, a major scab resistance gene was introduced. Fhb1 It can enhance resistance to Fusarium head blight to "high resistance," surpassing that of Sumai 3. This is achieved through the use of effective molecular markers. TaFhb‑2DL and Fhb1 The aggregation of these technologies can quickly and effectively breed varieties highly resistant to Fusarium head blight.

This invention discloses molecular markers, primers, and their applications closely linked to the major quantitative trait locus for wheat resistance to Fusarium head blight (Fusarium head blight) Qfhb.jaas-7DS, belonging to the field of agricultural biotechnology. This invention identifies a novel, stable major quantitative trait locus (QTL) for Fusarium head blight resistance on the short arm of chromosome 7D, named Qfhb.jaas-7DS. To effectively utilize this resistance locus, two KASP markers, KASP-109827800 and KASP-109891380, closely linked to this locus, were designed and developed based on the 7D chromosome genome sequence and SNP sequence information. These two markers serve as molecular markers for the Fusarium head blight resistance gene on the short arm of wheat chromosome 7D, and can be used for high-throughput detection of this locus, as well as assisted selection of Fusarium head blight resistant wheat varieties carrying this gene, thereby accelerating the breeding process of disease-resistant varieties.

The invention provides an engineering bacterium for high-yield gibberellic acid GA3 as well as a construction method and application thereof, and belongs to the technical field of gene engineering. According to the invention, gibberella fujikouensis QJ47-9 is used as an original strain, and a DES gene overexpressed strain marked as a DESOE strain is obtained by overexpressing and amplifying a gene DES encoding DES dehydrogenase. On the basis of the DESOE strain, the P450-3 monooxygenase is subjected to key amino acid mutation, the selected mutation sites are F97Y, T220A, V236I and D350Q, and the mutant strain is marked as DESOE / p450-3 delta. After fermentation culture is carried out for 7 days at the temperature of 28 DEG C and the speed of 250 rpm, the yield of GA3 is increased to 2.51 g / L from 1.53 g / L of an initial strain, and the increasing rate is 64.05%; meanwhile, the yield of a byproduct GA1 is reduced to 0.06 g / L.

The invention discloses wheat gibberella and application thereof, and belongs to the technical field of biology. A 26SrDNA (deoxyribonucleic acid) of the gene has a gene sequence as shown in SEQ ID No. 1; the gibberella zearalenone obtained through separation, screening and mutation can be efficiently synthesized into the gibberella zearalenone through liquid fermentation and solid fermentation, the highest yields of the gibberella zearalenone reach 11.5 g / L and 8.3 g / kg respectively, the production efficiency of the gibberella zearalenone is remarkably improved, and a foundation is laid for application of the gibberella zearalenone as a medicine raw material.

The invention relates to the technical field of prevention and control of gibberellic disease, in particular to a lysozyme compound preparation for targeted degradation of DON toxin and application of the lysozyme compound preparation in prevention and control of wheat gibberellic disease. The lysozyme compound preparation for targeted degradation of DON toxin is prepared from the following raw materials in parts by weight: 10-30 parts of a first enzyme source, 3-10 parts of a second enzyme source, 2-8 parts of carboxymethyl chitosan, 1-4 parts of an auxiliary agent and 1-3 parts of trehalose, the lysozyme compound preparation for targeted degradation of DON toxin prepared by the invention not only synchronously inhibits growth of gibberella and degrades DON toxin, but also improves the adhesion and infiltration capacity on the surface of wheat ears, the lasting period of pesticide effect reaches 21 days due to embedding of carboxymethyl chitosan, the DON residue in grains is less than 0.5 ppm, the European Union standard is met, the thousand seed weight and the yield increase per mu are improved, and the application prospect is wide. And the quality is effectively ensured.

The application discloses an arecoline ester derivative, a preparation method and application thereof, relates to the technical field of chemical pesticides, and has a brand-new molecular structure, and the bacteriostatic activity and insecticidal activity of the arecoline ester derivative are significantly enhanced compared with arecoline; the arecoline ester derivative has certain inhibiting effects on Puccinia recondita, Diplodia natalensis, Gibberella saubinetii, Pyricularia grisea, Phomopsis vexans, Physalospora piricola, Pestalotiopsis versiflorea, and Colletotrichum gloeosporioides; and the arecoline ester derivative also has certain insecticidal activity on Aedes aegypti larvae.