Lysozyme compound preparation for targeted degradation of DON toxin and application of lysozyme compound preparation in prevention and control of wheat scab

Abstract

The invention relates to the technical field of prevention and control of gibberellic disease, in particular to a lysozyme compound preparation for targeted degradation of DON toxin and application of the lysozyme compound preparation in prevention and control of wheat gibberellic disease. The lysozyme compound preparation for targeted degradation of DON toxin is prepared from the following raw materials in parts by weight: 10-30 parts of a first enzyme source, 3-10 parts of a second enzyme source, 2-8 parts of carboxymethyl chitosan, 1-4 parts of an auxiliary agent and 1-3 parts of trehalose, the lysozyme compound preparation for targeted degradation of DON toxin prepared by the invention not only synchronously inhibits growth of gibberella and degrades DON toxin, but also improves the adhesion and infiltration capacity on the surface of wheat ears, the lasting period of pesticide effect reaches 21 days due to embedding of carboxymethyl chitosan, the DON residue in grains is less than 0.5 ppm, the European Union standard is met, the thousand seed weight and the yield increase per mu are improved, and the application prospect is wide. And the quality is effectively ensured.

Description

Technical Field

[0001] This invention relates to the field of Fusarium head blight control technology, specifically to a lysozyme compound preparation that targets and degrades DON toxin and its application in the control of wheat Fusarium head blight. Background Technology

[0002] Wheat scab, a global agricultural problem often referred to as "wheat cancer," is caused by the pathogen *Fusarium graminearum*, which not only drastically reduces wheat yields but also produces highly toxic deoxynivalenol (DON), posing a serious threat to food safety. This toxin is a colorless, needle-like crystal with extremely high heat and acid stability, making it ineffective in conventional food processing methods. It can only be partially degraded through strong alkali or high-pressure treatment. Ingestion of contaminated grains by humans or animals can cause symptoms known as "grain intoxication," including vomiting and diarrhea.

[0003] For the prevention and control of wheat scab, the main methods are chemical control and biological control. Among them, chemical control is still the main means of controlling scab. Commonly used agents such as tebuconazole and cyazofamid can inhibit mycelial growth to a certain extent, but they have three major defects: First, they have no detoxification function, they can only prevent the spread of pathogens, but cannot degrade the DON toxins that have been produced, and are ineffective in controlling toxin residues; Second, resistance outbreaks occur, and long-term single use leads to the rapid evolution of pathogen resistance; Third, the risk of pesticide residues is prominent, and the residues of the chemical agents themselves and the inhibitory effect on toxin degradation can easily lead to the exceedance of grain safety indicators.

[0004] Wildlife control technologies have garnered significant attention due to their environmental friendliness, but existing solutions all have obvious shortcomings: 1. Antibacterial agents: Represented by Bacillus subtilis strain B6, these agents inhibit the growth of Fusarium graminearum by secreting antibacterial proteins, and their field control efficacy can reach a moderate level. However, they do not have the ability to degrade DON toxins and cannot achieve synergistic control of "disease prevention + detoxification". 2. Detoxification agents: Although biodegradable agents such as lactic acid bacteria fermentation broth can degrade DON under laboratory conditions, their actual control efficacy is less than 40% due to problems such as poor field stability and long action period, which is difficult to meet production needs. 3. Enzyme preparation improvement: Existing lysozyme and chitosan compound solutions are mainly designed for fruit and vegetable diseases, without taking into account the special structure of wheat ears - the thick waxy layer of the husk and the narrow gaps, which makes it difficult for the solution to spread and penetrate. Furthermore, the enzyme activity has not been optimized for the structural characteristics of Fusarium graminearum cell walls, which are rich in β-1,3-glucan, resulting in a significant reduction in the control effect.

[0005] Based on this, the present invention provides a lysozyme compound preparation for targeted degradation of DON toxin and its application in the control of wheat scab, so as to solve the above-mentioned technical problems. Summary of the Invention

[0006] The purpose of this invention is to provide a lysozyme compound preparation for targeted degradation of DON toxin and its application in the control of wheat scab. The prepared lysozyme compound preparation for targeted degradation of DON toxin not only simultaneously inhibits the growth of Fusarium graminearum and degrades DON toxin, but also enhances its adhesion and penetration ability on the surface of wheat ears. Carboxymethyl chitosan encapsulation makes the efficacy last for 21 days, with DON residue in grains <0.5ppm, which meets EU standards. The thousand-grain weight and yield per acre are improved, effectively ensuring its quality and efficacy.

[0007] To achieve the above objectives, the present invention provides the following technical solution: The first aspect of the present invention provides a lysozyme complex preparation for targeted degradation of DON toxin, which is composed of the following raw materials in parts by weight: 10-30 parts of a first enzyme source, 3-10 parts of a second enzyme source, 2-8 parts of carboxymethyl chitosan, 1-4 parts of adjuvants and 1-3 parts of trehalose. The preparation process of the lysozyme complex formulation for targeting and degrading DON toxin is as follows: Accurately weigh the first enzyme source, the second enzyme source, carboxymethyl chitosan, the auxiliary agent, and trehalose, and set aside. The second enzyme source and trehalose were freeze-dried, and then the first enzyme source was added and mixed in a stirring device to obtain a mixture. Carboxymethyl chitosan is dissolved in deionized water to prepare a carboxymethyl chitosan solution. Then, a mixture is added to the solution and stirred until homogeneous. The solution is then sprayed into a drying device using a high-pressure sprayer to evaporate the deionized water and obtain the coating. Adding adjuvants to the encapsulated material, stirring and mixing, and then adjusting the volume, a lysozyme complex preparation for targeted degradation of DON toxin was prepared.

[0008] A further feature of this invention is that the first enzyme source is selected from either recombinant lysozyme or marine cryogenic lysozyme.

[0009] A further feature of this invention is that the second enzyme source is selected from either DON degrading enzyme or Nocardioides strain extract.

[0010] The present invention further specifies that the specific extraction process of the Nocardioides strain extract is as follows: Strain cultivation: Nocardioides strain was inoculated into a 250 mL Erlenmeyer flask containing 50 mL of medium (10.0 g tryptone, 5.0 g yeast extract, 5.0 g NaCl, 0.1 g (concentration 100 mg / L) DON, and diluted to 1 L with deionized water) and cultured at 30℃ and 180 r / min for 24 h with shaking. At an inoculation rate of 5% (v / v), the obtained activated bacterial solution was inoculated into a 2 L Erlenmeyer flask containing 500 mL of culture medium and cultured at 30℃ and 190 r / min for 16 h to obtain a highly active seed solution. Fermentation for enzyme production: At an inoculation rate of 10% (v / v), the highly active seed culture was inoculated into a fermenter containing 500 mL of fermentation medium (15.0 g glucose, 3.0 g ammonium nitrate, 1.0 g KH2PO4, 2.0 g K2HPO4·3H2O, 0.5 g MgSO4·7H2O, 0.5 g (concentration 50 mg / L) DON, with the volume adjusted to 10 L by deionized water, and the pH adjusted to 7.2 by 1 mol / L NaOH). Fermentation was carried out at 30℃ and 200 r / min for 34 h to obtain the highly active seed culture. The obtained fermentation broth was centrifuged at 8200 r / min and 4℃ for 17 min, and the bacterial cells were collected. The homogenizer was used for crushing at a pressure of 800 bar, and the crushing was repeated 3 times. The resulting crushed liquid was centrifuged at 11000 r / min and 4℃ for 22 min, and the supernatant was collected. Ammonium sulfate was slowly added to a saturation of 50%, and the mixture was stirred at 4°C for 3 hours. Then, it was centrifuged at 13000 r / min for 15 minutes, the precipitate was collected, and the precipitate was reconstituted with 50 mL of pH 7.0 phosphate buffer (0.02 mol / L) to obtain the concentrated solution. The concentrate was placed into a dialysis bag with a molecular weight cutoff of 10 kDa and dialyzed in 1200 ml of phosphate buffer at 4°C for 25 h to obtain the dialysate. Equilibrate the DEAE-52 ion exchange chromatography column with pH 7.0 phosphate buffer (0.02 mol / L); The dialysate was loaded at a flow rate of 1 mL / min. First, the impurities were eluted with the equilibration buffer, and then the equilibration buffer containing 0.3 mol / L NaCl was used for gradient elution (flow rate of 1 mL / min). The DON degradation activity peak solution was collected. The active peak solution was concentrated using an ultrafiltration membrane with a molecular weight cutoff of 10 kDa to obtain the Nocardioides strain extract.

[0011] A further feature of this invention is that the auxiliary agent is selected from either the first composite agent or the second composite agent.

[0012] The present invention is further configured such that the first composite agent is composed of tea saponin and polyglycerol ester in a mass ratio of 1:2.

[0013] The present invention is further configured such that: the second composite agent is composed of sophorolipid and ultraviolet protectant in a mass ratio of 1:0.2 to 0.4.

[0014] A further feature of this invention is that the ultraviolet protectant is 2-hydroxy-4-methoxybenzophenone.

[0015] A further feature of the present invention is that the mass concentration of the carboxymethyl chitosan solution is 2-5%.

[0016] A further feature of this invention is that the solution used for volume adjustment is a phosphate buffer solution with a pH of 5.5.

[0017] A second aspect of the present invention also provides the application of the above-mentioned lysozyme complex preparation that targets and degrades DON toxin in the control of wheat scab.

[0018] Compared with the prior art, the beneficial effects of the present invention are: This invention uses a first enzyme source, a second enzyme source, carboxymethyl chitosan, adjuvants, and trehalose as raw materials to prepare a lysozyme complex formulation that targets and degrades DON toxin. In the control of wheat scab, the lysozyme destroys the cell wall of Fusarium graminearum, promotes the entry of DON-degrading enzyme into the fungus, hydrolyzes the toxin to synthesize the key enzyme Tri5, and the carboxymethyl chitosan microcapsules release the dual enzymes slowly to resist rainwater erosion on the surface of the wheat ear. Tea saponin reduces the tension of the waxy layer on the surface of the wheat ear and increases the spreading area of ​​the pesticide solution. Polyglycerol esters enhance the penetration of the pesticide into the glumes. The DON-degrading enzyme specifically recognizes the epoxy groups of the toxin and breaks the C12-C13 bonds to achieve detoxification. The prepared lysozyme compound formulation targeting the degradation of DON toxin not only simultaneously inhibits the growth of Fusarium graminearum and degrades DON toxin, but also enhances its adhesion and penetration ability on the surface of wheat ears. Carboxymethyl chitosan encapsulation extends the efficacy to 21 days, with DON residue in the grains <0.5 ppm, meeting EU standards. Thousand-grain weight and yield per acre are improved, effectively ensuring the quality and efficacy of the product. This indicates that the lysozyme compound formulation targeting the degradation of DON toxin provided by this invention has a broader market prospect and is more suitable for widespread application. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the dual-enzyme action mechanism in Example 1.

[0020] Figure 2 The image shows a SEM image of the lysozyme complex preparation prepared in Example 1. Detailed Implementation

[0021] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0022] Example 1: This embodiment provides a lysozyme complex formulation for targeted degradation of DON toxin, which is composed of the following raw materials in parts by weight: 10 parts of first enzyme source, 3 parts of second enzyme source, 2 parts of carboxymethyl chitosan, 1 part of adjuvant and 1 part of trehalose.

[0023] The first enzyme source is recombinant lysozyme.

[0024] The second enzyme source is DON degrading enzyme.

[0025] The additive is selected from the first composite agent.

[0026] Furthermore, the first composite agent is composed of tea saponin and polyglycerol ester in a mass ratio of 1:2.

[0027] Furthermore, this embodiment provides a method for preparing the lysozyme complex formulation that targets and degrades DON toxin, the specific process of which is as follows: Accurately weigh the first enzyme source, the second enzyme source, carboxymethyl chitosan, the auxiliary agent, and trehalose, and set aside. The second enzyme source and trehalose were freeze-dried, and then the first enzyme source was added and mixed in a stirring device at a speed of 20 r / min for 30 min to obtain a mixture. Carboxymethyl chitosan was dissolved in deionized water to prepare a carboxymethyl chitosan solution with a mass concentration of 2%. Then, a mixture was added to the solution and stirred until homogeneous. The stirring speed was 40 r / min and the stirring time was 30 min. The mixture was then sprayed into a drying device using a high-pressure spray device to evaporate the deionized water and obtain the encapsulated material. The high-pressure spraying equipment sprays into the drying equipment at a flow rate of 1 mL / min at a temperature of 80°C. The hot air in the drying equipment enters from the bottom and comes into full contact with the droplets at a flow rate of 0.5 m / s. Additives were added to the package, and the mixture was stirred and then brought to a final volume. The solution used for final volume bringing was a phosphate buffer solution with a pH of 5.5. This yielded a lysozyme complex preparation that targets and degrades DON toxin.

[0028] Example 2: This embodiment provides a lysozyme complex formulation for targeted degradation of DON toxin, which is composed of the following raw materials in parts by weight: 20 parts of first enzyme source, 6 parts of second enzyme source, 5 parts of carboxymethyl chitosan, 2 parts of adjuvant and 2 parts of trehalose.

[0029] The first enzyme source is recombinant lysozyme.

[0030] The second enzyme source is DON degrading enzyme.

[0031] The additive is selected from the first composite agent.

[0032] Furthermore, the first composite agent is composed of tea saponin and polyglycerol ester in a mass ratio of 1:2.

[0033] Furthermore, this embodiment provides a method for preparing the lysozyme complex formulation that targets and degrades DON toxin, the specific process of which is as follows: Accurately weigh the first enzyme source, the second enzyme source, carboxymethyl chitosan, the auxiliary agent, and trehalose, and set aside. The second enzyme source and trehalose were freeze-dried, and then the first enzyme source was added and mixed in a stirring device at a speed of 30 r / min for 35 min to obtain a mixture. Carboxymethyl chitosan was dissolved in deionized water to prepare a carboxymethyl chitosan solution with a mass concentration of 3%. Then, a mixture was added to the solution and stirred until homogeneous. The stirring speed was 50 r / min and the stirring time was 35 min. The mixture was then sprayed into a drying device using a high-pressure spray device to evaporate the deionized water and obtain the encapsulated material. The high-pressure spraying equipment sprays the air into the drying equipment at a flow rate of 2 mL / min at a temperature of 100°C. The hot air in the drying equipment enters from the bottom and comes into full contact with the droplets at a flow rate of 1.0 m / s. Additives were added to the package, and the mixture was stirred and then brought to a final volume. The solution used for final volume bringing was a phosphate buffer solution with a pH of 5.5. This yielded a lysozyme complex preparation that targets and degrades DON toxin.

[0034] Example 3: This embodiment provides a lysozyme complex formulation for targeted degradation of DON toxin, which is composed of the following raw materials in parts by weight: 30 parts of first enzyme source, 10 parts of second enzyme source, 8 parts of carboxymethyl chitosan, 4 parts of adjuvants and 3 parts of trehalose.

[0035] The first enzyme source is recombinant lysozyme.

[0036] The second enzyme source is DON degrading enzyme.

[0037] The additive is selected from the first composite agent.

[0038] Furthermore, the first composite agent is composed of tea saponin and polyglycerol ester in a mass ratio of 1:2.

[0039] Furthermore, this embodiment provides a method for preparing the lysozyme complex formulation that targets and degrades DON toxin, the specific process of which is as follows: Accurately weigh the first enzyme source, the second enzyme source, carboxymethyl chitosan, the auxiliary agent, and trehalose, and set aside. The second enzyme source and trehalose were freeze-dried, and then the first enzyme source was added and mixed in a stirring device at a speed of 40 r / min for 40 min to obtain a mixture. Carboxymethyl chitosan was dissolved in deionized water to prepare a carboxymethyl chitosan solution with a mass concentration of 5%. Then, a mixture was added to the solution and stirred until homogeneous. The stirring speed was 60 r / min and the stirring time was 40 min. The mixture was then sprayed into a drying device using a high-pressure spray device to evaporate the deionized water and obtain the encapsulated material. The high-pressure spraying equipment sprays the air into the drying equipment at a temperature of 120°C at a flow rate of 3 mL / min. The hot air in the drying equipment enters from the bottom and comes into full contact with the droplets at a flow rate of 1.5 m / s. Additives were added to the package, and the mixture was stirred and then brought to a final volume. The solution used for final volume bringing was a phosphate buffer solution with a pH of 5.5. This yielded a lysozyme complex preparation that targets and degrades DON toxin.

[0040] Example 4: This embodiment provides a lysozyme complex formulation for targeted degradation of DON toxin, which is composed of the following raw materials in parts by weight: 20 parts of first enzyme source, 6 parts of second enzyme source, 5 parts of carboxymethyl chitosan, 2 parts of adjuvant and 2 parts of trehalose.

[0041] The first enzyme source is marine low-temperature lysozyme.

[0042] The second enzyme source was an extract from the Nocardioides strain.

[0043] The auxiliary agent is selected from the second compound agent.

[0044] Furthermore, the second compound agent is composed of sophorolipid and ultraviolet protectant in a mass ratio of 1:0.3.

[0045] Furthermore, this embodiment provides a method for preparing the lysozyme complex formulation that targets and degrades DON toxin, the specific process of which is as follows: Accurately weigh the first enzyme source, the second enzyme source, carboxymethyl chitosan, the auxiliary agent, and trehalose, and set aside. The second enzyme source and trehalose were freeze-dried, and then the first enzyme source was added and mixed in a stirring device at a speed of 30 r / min for 35 min to obtain a mixture. Carboxymethyl chitosan was dissolved in deionized water to prepare a carboxymethyl chitosan solution with a mass concentration of 3%. Then, a mixture was added to the solution and stirred until homogeneous. The stirring speed was 50 r / min and the stirring time was 35 min. The mixture was then sprayed into a drying device using a high-pressure spray device to evaporate the deionized water and obtain the encapsulated material. The high-pressure spraying equipment sprays the air into the drying equipment at a flow rate of 2 mL / min at a temperature of 100°C. The hot air in the drying equipment enters from the bottom and comes into full contact with the droplets at a flow rate of 1.0 m / s. Additives were added to the package, and the mixture was stirred and then brought to a final volume. The solution used for final volume bringing was a phosphate buffer solution with a pH of 5.5. This yielded a lysozyme complex preparation that targets and degrades DON toxin.

[0046] Comparative Example 1: The difference from Example 1 is that this example does not contain any additives.

[0047] Comparative Example 2: The difference from Example 1 is that this example does not contain carboxymethyl chitosan.

[0048] Comparative Example 3: The difference from Example 1 is that 25% cyazofamid suspension concentrate (commercially available, Jiangsu Provincial Pesticide Research Institute Co., Ltd.) is used instead of lysozyme compound preparation in this example.

[0049] Performance testing: Experimental conditions: Huang-Huai wheat region, flowering stage, temperature 20–28℃, relative humidity 60–70%, sunshine 9–11 h / day; wheat variety: 'Jimai 22', density 180,000 plants / mu; pathogen background: artificial inoculation with Fusarium graminearum, sprayed one day before flowering stage, concentration 1×10⁻⁶. 6 Spores / mL.

[0050] Application procedures for Examples 1-4 and Comparative Examples 1-3: Spray once 3 days before flowering and once 7 days after flowering; use drones for aerial spraying at a flight altitude of 1.5m and a speed of 4m / s, focusing on spraying the panicle; dosage: 2L per mu (667 square meters), the concentration of Examples 1-4 and Comparative Examples 1-2 is 10X (formulation diluted 10 times), and 40mL per mu is used in Comparative Example 3, diluted to 2L; re-spray: if it rains 4 hours after the application 7 days after flowering, re-spray once the next day, with the same dosage as the first time, and a blank control group is set up at the same time.

[0051] Fusarium wilt inhibition rate test: Inhibition rate = (Disease rate of blank control - Disease rate of treated control) / Disease rate of blank control × 100%, where disease rate = number of diseased ears / total number of ears × 100%; Duration of effect: number of days when the inhibition rate is ≥ 85%.

[0052] DON Degradation and Residue: Degradation Rate = (Blank Residue - Treated Residue) / Blank Residue × 100%; 1000-grain weight: average weight (g) of 1000 grains; Yield per mu = yield per plot × 666.7 / plot area; Increase in yield = (Processed output - Blank output) / Blank output × 100%; The obtained test data are recorded in Tables 1 and 2 below: Table 1. Performance test results of inhibition rate of each group against Fusarium moniliforme. Group Example 1 group Example 2 group Example 3 group Example 4 group Comparison 1 group Comparison 2 group Comparison 3 group 7 days after application 85.8 86.5 88.1 86.3 72.4 82.3 83.4 14 days after application 90.3 92.2 93.4 91.4 75.1 77.5 86.1 21 days after application 01.5 93.3 94.4 92.4 76.5 65.3 88.0 Effective period 21 21 21 21 21 14 21 Table 2. Test results of each group 21 days after drug administration. Group Example 1 group Example 2 group Example 3 group Example 4 group Comparison 1 group Comparison 2 group Comparison 3 group DON degradation rate (%) 85.6 87.2 89.1 86.7 72.3 78.2 0 Grain DON residue (ppm) 0.43 0.42 0.37 0.45 0.82 1.2 2.3 Thousand seed weight (g) 42.5 43.2 43.5 43.6 40.1 41.3 39.8 Yield per mu (kg) 485 497 503 491 443 457 445 Yield increase rate (%) 17.6 20.3 21.2 19.3 9.9 13.4 10.1 By comparing and analyzing the relevant data in Tables 1 and 2, it can be seen that the lysozyme compound preparation for targeted degradation of DON toxin prepared in this invention not only simultaneously inhibits the growth of Fusarium wilt and degrades DON toxin, but also enhances its adhesion and penetration ability on the surface of wheat ears. The carboxymethyl chitosan encapsulation extends the efficacy to 21 days, with DON residue in the grains <0.5 ppm, meeting EU standards. The thousand-grain weight and yield per acre are improved, effectively ensuring its quality and efficacy. This indicates that the lysozyme compound preparation for targeted degradation of DON toxin provided by this invention has a broader market prospect and is more suitable for promotion.

[0053] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0054] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A lysozyme complex formulation for targeted degradation of DON toxin, characterized in that, It is composed of the following raw materials in parts by weight: 10-30 parts of primary enzyme source, 3-10 parts of secondary enzyme source, 2-8 parts of carboxymethyl chitosan, 1-4 parts of adjuvants and 1-3 parts of trehalose; The preparation process of the lysozyme complex formulation for targeting and degrading DON toxin is as follows: Accurately weigh the first enzyme source, the second enzyme source, carboxymethyl chitosan, the auxiliary agent, and trehalose, and set aside. The second enzyme source and trehalose were freeze-dried, and then the first enzyme source was added and mixed in a stirring device to obtain a mixture. Carboxymethyl chitosan is dissolved in deionized water to prepare a carboxymethyl chitosan solution. Then, a mixture is added to the solution and stirred until homogeneous. The solution is then sprayed into a drying device using a high-pressure sprayer to evaporate the deionized water and obtain the coating. Adding adjuvants to the encapsulated material, stirring and mixing, and then adjusting the volume, a lysozyme complex preparation for targeted degradation of DON toxin was prepared.

2. The lysozyme complex formulation for targeted degradation of DON toxin according to claim 1, characterized in that, The first enzyme source can be either recombinant lysozyme or marine cryogenic lysozyme.

3. The lysozyme complex formulation for targeted degradation of DON toxin according to claim 1, characterized in that, The second enzyme source can be selected from either DON degrading enzyme or Nocardioides strain extract.

4. The lysozyme complex formulation for targeted degradation of DON toxin according to claim 1, characterized in that, The additive is selected from either the first composite agent or the second composite agent.

5. The lysozyme complex formulation for targeted degradation of DON toxin according to claim 4, characterized in that, The first composite agent is composed of tea saponin and polyglycerol ester in a mass ratio of 1:

2.

6. The lysozyme complex formulation for targeted degradation of DON toxin according to claim 4, characterized in that, The second compound agent is composed of sophorolipid and ultraviolet protectant in a mass ratio of 1:0.2 to 0.

4.

7. The lysozyme complex formulation for targeted degradation of DON toxin according to claim 1, characterized in that, The mass concentration of the carboxymethyl chitosan solution is 2-5%.

8. The lysozyme complex formulation for targeted degradation of DON toxin according to claim 1, characterized in that, The solution used for volume adjustment is a phosphate buffer solution with a pH of 5.

5.

9. The application of the lysozyme complex preparation for targeting and degrading DON toxin according to any one of claims 1 to 8 in the prevention and control of wheat scab.

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