Plant polyphenol bacteriostatic anti-allergic agent and extraction method thereof

Abstract

The invention relates to the technical field of skin care product raw materials, and particularly discloses a plant polyphenol bacteriostatic anti-allergic agent and an extraction method thereof. The extraction method of the plant polyphenol bacteriostatic and anti-allergic agent comprises the following steps: 1) crushing and mixing almonds, licorice roots, wild chrysanthemum flowers, radix sophorae flavescentis, sweet wormwood roots, magnolia flowers and stephania tetrandra to obtain a composite plant; 2) mixing the composite plant with water, heating and extracting to obtain a pre-extract; 3) cooling the pre-extract, adding the pre-extract into a compound zymophyte solution, and fermenting for 20-22 days to obtain a fermented compound; (4) filtering and sterilizing the fermentation compound to obtain fermentation liquor; 5) concentrating and drying the fermentation broth to obtain a composite plant extract, and 6) mixing the composite plant extract, water and a preservative to obtain the plant polyphenol bacteriostatic and anti-allergic agent, the composite fermentation broth is prepared by compounding pichia mannici, gibberella avenae, staphylococcus sciuri and a liquid culture medium, and the plant polyphenol bacteriostatic and anti-allergic agent has the advantage of further improving the bacteriostatic effect so as to better improve the acne removal effect.

Description

Technical Field

[0001] The present invention relates to the field of skin care product raw materials, and in particular to a plant polyphenol antibacterial and anti-allergic agent and an extraction method thereof. Background Art

[0002] Acne is one of the common skin problems, mainly caused by the growth of bacteria in hair follicles. Usually, antibacterial ingredients are added to acne-removing skin care products. However, chemically synthesized antibacterial ingredients such as triclosan and imidazolidinyl urea have strong antibacterial effects. However, long-term use may pose potential risks to human health, especially in people with sensitive skin, and can easily cause allergic reactions. Therefore, antibacterial ingredients derived from natural plants are gradually favored by consumers because of their mild and safe characteristics.

[0003] However, the antibacterial effect of existing natural plant extracts is relatively general, and it is difficult to quickly kill Propionibacterium acnes in the skin, resulting in a slow acne removal effect and difficulty in meeting the needs of consumers. Therefore, there is still room for improvement. Summary of the Invention

[0004] In order to further improve the antibacterial efficacy and better enhance the acne removal effect, the present application provides a plant polyphenol antibacterial and anti-allergic agent and an extraction method thereof.

[0005] In a first aspect, the present application provides a method for extracting plant polyphenol antibacterial and anti-allergic agents, which adopts the following technical solution: A method for extracting plant polyphenol antibacterial and anti-allergic agents, comprising the following steps: Step 1) crushing and mixing almonds, liquorice roots, wild chrysanthemums, sophora flavescens roots, artemisia annua roots, magnolia flowers, and stephania tetrandra to obtain a composite plant; Step 2), mixing the composite plant with water, heating and extracting, to obtain a pre-extract; Step 3), cooling the pre-extract and adding the composite fermentation liquid, and fermenting for 20-22 days to obtain a fermentation complex; Step 4), filtering and sterilizing the fermentation complex to obtain a fermentation broth; Step 5), concentrating and drying the fermentation liquid to obtain a composite plant extract; Step 6), mixing the composite plant extract, water, and preservative to obtain a plant polyphenol antibacterial and anti-allergic agent; The composite fermentation bacterial liquid is prepared by compounding Pichia mansoni, Gibberella avenae, Staphylococcus squirrel and liquid culture medium; The OD value of the composite fermentation bacterial liquid is ≥1.

[0006] By adopting the above technical scheme, by using a compound extraction of almond, licorice root, wild chrysanthemum, sophora flavescens root, artemisia annua root, magnolia flower, and stephania tetrandra and fermenting with Pichia mansoni, Gibberella avenae, and Staphylococcus squirrel, the obtained plant polyphenol antibacterial and anti-allergic agent has significant efficacy in inhibiting Propionibacterium acnes, and has a good soothing effect, can quickly relieve skin discomfort, and has good efficacy in removing acne and repairing skin.

[0007] Preferably, in step 1), the mass ratio of almond, liquorice root, wild chrysanthemum, sophora flavescens root, artemisia annua root, magnolia flower and stephania tetrandra is 2.5-3.5:2.5-3.5:1.5-2.5:1.5-2.5:1.5-2.5:1.5-2.5:0.5-1.5.

[0008] By adopting the above technical solution and specifically selecting the mass ratios of almond, licorice root, wild chrysanthemum, sophora flavescens root, artemisia annua root, magnolia flower, and stephania tetrandra, the active ingredients can better cooperate with each other, thereby achieving better antibacterial and soothing effects and faster acne repair effects.

[0009] Preferably, in step 2), the mass ratio of the composite plant to water is 1:5-6.

[0010] By adopting the above technical solution and specifically selecting the mass ratio of the composite plant and water, the extraction effect is better and the product quality is better.

[0011] Preferably, in step 2), the mixture is heated to 80-85° C. and extracted for 6-8 hours.

[0012] By adopting the above technical solution and specifically selecting the extraction temperature and extraction time, the extraction effect is improved, which can better cooperate with the fermentation process, and the obtained plant polyphenol antibacterial and anti-allergic agent has better efficacy.

[0013] Preferably, in step 3), the temperature is kept at 30-32° C. and the fermentation is carried out for 20-22 days.

[0014] By adopting the above technical solution and specifically selecting the fermentation temperature, the activity of each bacteria is higher and the fermentation effect is better, which can better improve the antibacterial and anti-allergic effects of the plant polyphenol antibacterial and anti-allergic agent.

[0015] Preferably, in step 6), the mass ratio of the composite plant extract, water, and preservative is 1.725-2.78:12.12-13.2:0.075-0.1.

[0016] By adopting the above technical solution and specifically selecting the mass ratio of the composite plant extract, water and preservatives, the product has higher stability, better product quality and is easy to be combined with cosmetics.

[0017] Preferably, in the composite fermentation broth, the mass ratio of Pichia mansoni, Gibberella avenae, Staphylococcus sciuri and liquid culture medium is 1-2:3-4:2-3:1000.

[0018] By adopting the above technical solution and specifically selecting the mass ratios of Pichia mansoni, Gibberella avenae, Staphylococcus squirrel and liquid culture medium, the bacteria are combined in a specific ratio, the fermentation effect is better, and the fermentation product can be better combined with the active ingredient in a specific ratio, thereby improving the antibacterial and anti-allergic effects of the plant polyphenol antibacterial and anti-allergic agent.

[0019] Preferably, the liquid culture medium is a mixture of water, peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, sodium chloride, lettuce juice, kale juice, and spinach juice.

[0020] By adopting the above technical solution, adding lettuce juice, kale juice and spinach juice to the liquid culture medium can help improve the activity of various bacteria, resulting in better fermentation effect, and the prepared plant polyphenol antibacterial and anti-allergic agent has better antibacterial and anti-allergic effects.

[0021] Preferably, the mass ratio of water, peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, sodium chloride, lettuce juice, kale juice and spinach juice in the liquid culture medium is 766.6-807.4:18-22:18-22:1.8-2.2:0.8-1.2:4-6:50-60:50-60:50-60.

[0022] By adopting the above technical solution and specifically selecting the mass ratios of water, peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, sodium chloride, lettuce juice, kale juice, and spinach juice in the liquid culture medium, the efficiency of bacterial culture is increased, the effect of fermenting the composite plant with the composite fermentation bacterial liquid is better, and the product quality is better.

[0023] Preferably, the mass ratio of the composite plant to the composite fermentation bacteria liquid is 10:1-2.

[0024] By adopting the above technical solution and specifically selecting the mass ratio of the composite plant and the composite fermentation bacterial liquid, a better fermentation effect can be ensured while reducing material waste, thereby having higher economic value.

[0025] In a second aspect, the present application provides a plant polyphenol antibacterial and anti-allergic agent, which adopts the following technical solution: A plant polyphenol antibacterial and anti-allergic agent is prepared by the above-mentioned plant polyphenol antibacterial and anti-allergic agent extraction method.

[0026] By adopting the above technical solutions, plant polyphenol antibacterial and anti-allergic agents can have significant antibacterial and anti-allergic effects, can quickly eliminate acne, relieve skin discomfort, and have better skin repair effects, thus meeting the needs of consumers well.

[0027] In summary, this application has the following beneficial effects: 1. Since the present application adopts a compound extraction of almond, licorice root, wild chrysanthemum, sophora flavescens root, artemisia annua root, magnolia flower, and stephania tetrandra and ferments it with Pichia mansoni, Gibberella avenae, and Staphylococcus squirrel, the obtained plant polyphenol antibacterial and anti-allergic agent has a significant effect of inhibiting Propionibacterium acnes, and has a good soothing effect, can quickly relieve skin discomfort, and has a good effect of removing acne and repairing skin.

[0028] 2. In this application, the mass ratio of almond, licorice root, wild chrysanthemum, sophora flavescens root, artemisia annua root, magnolia flower and stephania tetrandra is preferably selected specifically so that the active ingredients can better cooperate with each other, thereby achieving better antibacterial and soothing effects and faster acne repair effects.

[0029] 3. In this application, it is preferred to specifically select the mass ratio of Pichia mansoni, Gibberella avenae, Staphylococcus squirrel, and liquid culture medium so that each bacteria is combined in a specific ratio, the fermentation effect is better, and the fermentation product can be better combined with the active ingredient in a specific ratio, thereby making the antibacterial and anti-allergic effects of the plant polyphenol antibacterial and anti-allergic agent better. DETAILED DESCRIPTION

[0030] The present application is further described in detail below with reference to the embodiments.

[0031] Example 1 A method for extracting plant polyphenol antibacterial and anti-allergic agents comprises the following steps: Step 1) chop 2.5 kg of almonds, 2.5 kg of licorice root, 1.5 kg of wild chrysanthemum, 1.5 kg of sophora flavescens root, 1.5 kg of artemisia annua root, 1.5 kg of magnolia flower, and 0.5 kg of stephania tetrandra, pass through a 40-mesh sieve, put into a stirred tank, rotate at 200 r / min, and stir for 5 minutes to obtain a composite plant.

[0032] Step 2) 57.5 kg of water was added to the composite plant, the mixture was stirred continuously at a speed of 60 r / min, heated to 80° C., and extracted for 6 h to obtain a pre-extract.

[0033] Step 3) The pre-extract was cooled to room temperature, 1.15 kg of composite fermentation bacterial liquid was added, stirring was continued at a speed of 10 r / min, the temperature was kept constant at 30° C., and fermentation was carried out for 20 days to obtain a fermentation complex.

[0034] Step 4), the fermentation complex is filtered through a filter cloth to obtain a filtrate, and the filtrate is sterilized by filtering through a 0.22 μm filter membrane to obtain a fermentation broth.

[0035] Step 5) The fermentation liquid is concentrated by rotary evaporation at 60° C. and 0.01 MPa to a paste, and the paste is powdered and dried to obtain a composite plant extract.

[0036] Step 6) 1.725 kg of the composite plant extract, 13.2 kg of water, and 0.075 kg of a preservative are mixed evenly to obtain a plant polyphenol antibacterial and anti-allergic agent.

[0037] The preparation method of the composite fermentation bacterial liquid is as follows: In step 01), 807.4 g of water, 18 g of peptone, 18 g of glucose, 1.8 g of potassium dihydrogen phosphate, 0.8 g of magnesium sulfate, 4 g of sodium chloride, 50 g of lettuce juice, 50 g of kale juice, and 50 g of spinach juice were mixed to obtain a liquid culture medium.

[0038] Step 02) Add 1 g of Pichia mansoni, 3 g of Gibberella avenae, and 2 g of Staphylococcus sciuridae to the liquid culture medium, and culture at a constant temperature of 30° C. until the OD value is 1 to obtain a composite fermentation bacterial liquid.

[0039] The almonds are sun-dried and purchased from Chongqing Chinese herbal medicine market.

[0040] Licorice root is a sun-dried product purchased from Chongqing Chinese herbal medicine market.

[0041] The wild chrysanthemum flowers were sun-dried and purchased from Chongqing Hengsheng Chinese Medicine Market.

[0042] The root of Sophora flavescens was sun-dried and purchased from Chongqing Hengsheng Chinese Medicine Market.

[0043] The roots of Artemisia annua L. are sun-dried products and were purchased from the Bund Chinese herbal medicine market in Chongqing.

[0044] Magnolia flowers and Stephania tetrandra were sun-dried products purchased from the Bund Chinese herbal medicine market in Chongqing.

[0045] The preservative is phenoxyethanol, which was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0046] Peptone was purchased from Haibo Biotechnology Co., Ltd.

[0047] Glucose was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0048] Potassium dihydrogen phosphate was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0049] Magnesium sulfate was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0050] Sodium chloride was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0051] Lettuce juice is made from fresh lettuce.

[0052] Kale juice is made by juicing fresh kale.

[0053] Spinach juice is made from fresh spinach juice.

[0054] Pichia mansoni was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D25102.

[0055] Gibberella avenae was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D13369.

[0056] Staphylococcus sciuridae was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D17780.

[0057] Example 2 A method for extracting plant polyphenol antibacterial and anti-allergic agents comprises the following steps: Step 1) chop 3kg of almonds, 3kg of licorice root, 2kg of wild chrysanthemum, 2kg of sophora flavescens root, 2kg of artemisia annua root, 2kg of magnolia flower, and 1kg of stephania tetrandra, pass through a 40-mesh sieve, put into a stirred tank, rotate at 200r / min, and stir for 5min to obtain a composite plant.

[0058] Step 2) 82.5 kg of water was added to the composite plant, stirred continuously at a speed of 60 r / min, heated to 83° C., and extracted for 7 h to obtain a pre-extract.

[0059] Step 3) The pre-extract was cooled to room temperature, 2.25 kg of composite fermentation bacterial liquid was added, stirring was continued at a speed of 10 r / min, the temperature was kept constant at 31° C., and fermentation was carried out for 21 days to obtain a fermentation complex.

[0060] Step 4), the fermentation complex is filtered through a filter cloth to obtain a filtrate, and the filtrate is sterilized by filtering through a 0.22 μm filter membrane to obtain a fermentation broth.

[0061] Step 5) The fermentation liquid is concentrated by rotary evaporation at 60° C. and 0.01 MPa to a paste, and the paste is powdered and dried to obtain a composite plant extract.

[0062] Step 6) 2.21 kg of the composite plant extract, 12.7 kg of water, and 0.09 kg of the preservative are mixed evenly to obtain a plant polyphenol antibacterial and anti-allergic agent.

[0063] The preparation method of the composite fermentation bacterial liquid is as follows: Step 01) 787 g of water, 20 g of peptone, 20 g of glucose, 2 g of potassium dihydrogen phosphate, 1 g of magnesium sulfate, 5 g of sodium chloride, 55 g of lettuce juice, 55 g of kale juice, and 55 g of spinach juice were mixed to obtain a liquid culture medium.

[0064] Step 02) Add 1.5 g of Pichia mansoni, 3.5 g of Gibberella avenae, and 2.5 g of Staphylococcus sciuridae to the liquid culture medium, and culture at a constant temperature of 31° C. until the OD value is 1, to obtain a composite fermentation bacterial liquid.

[0065] The almonds are sun-dried and purchased from Chongqing Chinese herbal medicine market.

[0066] Licorice root is a sun-dried product purchased from Chongqing Chinese herbal medicine market.

[0067] The wild chrysanthemum flowers were sun-dried and purchased from Chongqing Hengsheng Chinese Medicine Market.

[0068] The root of Sophora flavescens was sun-dried and purchased from Chongqing Hengsheng Chinese Medicine Market.

[0069] The roots of Artemisia annua L. are sun-dried products and were purchased from the Bund Chinese herbal medicine market in Chongqing.

[0070] Magnolia flowers and Stephania tetrandra were sun-dried products purchased from the Bund Chinese herbal medicine market in Chongqing.

[0071] The preservative is phenoxyethanol, which was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0072] Peptone was purchased from Haibo Biotechnology Co., Ltd.

[0073] Glucose was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0074] Potassium dihydrogen phosphate was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0075] Magnesium sulfate was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0076] Sodium chloride was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0077] Lettuce juice is made from fresh lettuce.

[0078] Kale juice is made by juicing fresh kale.

[0079] Spinach juice is made from fresh spinach juice.

[0080] Pichia mansoni was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D25102.

[0081] Gibberella avenae was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D13369.

[0082] Staphylococcus sciuridae was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D17780.

[0083] Example 3 A method for extracting plant polyphenol antibacterial and anti-allergic agents comprises the following steps: Step 1) 3.5 kg of almonds, 3.5 kg of liquorice root, 2.5 kg of wild chrysanthemum, 2.5 kg of sophora flavescens root, 2.5 kg of artemisia annua root, 2.5 kg of magnolia flower, and 1.5 kg of stephania tetrandra were chopped, passed through a 40-mesh sieve, and put into a stirred tank at a speed of 200 r / min and stirred for 5 minutes to obtain a composite plant.

[0084] Step 2) 111 kg of water was added to the composite plant, the mixture was stirred continuously at a speed of 60 r / min, heated to 85° C., and extracted for 8 h to obtain a pre-extract.

[0085] Step 3) The pre-extract was cooled to room temperature, 3.7 kg of composite fermentation bacterial liquid was added, stirring was continued at a speed of 10 r / min, the temperature was kept constant at 32° C., and fermentation was carried out for 22 days to obtain a fermentation complex.

[0086] Step 4), the fermentation complex is filtered through a filter cloth to obtain a filtrate, and the filtrate is sterilized by filtering through a 0.22 μm filter membrane to obtain a fermentation broth.

[0087] Step 5) The fermentation liquid is concentrated by rotary evaporation at 60° C. and 0.01 MPa to a paste, and the paste is powdered and dried to obtain a composite plant extract.

[0088] Step 6) 2.78 kg of the composite plant extract, 12.12 kg of water, and 0.1 kg of a preservative are mixed evenly to obtain a plant polyphenol antibacterial and anti-allergic agent.

[0089] The preparation method of the composite fermentation bacterial liquid is as follows: In step 01), 766.6 g of water, 22 g of peptone, 22 g of glucose, 2.2 g of potassium dihydrogen phosphate, 1.2 g of magnesium sulfate, 6 g of sodium chloride, 60 g of lettuce juice, 60 g of kale juice, and 60 g of spinach juice were mixed to obtain a liquid culture medium.

[0090] Step 02) Add 2g of Pichia mansoni, 4g of Gibberella avenae, and 3g of Staphylococcus sciuridae to the liquid culture medium, and culture at a constant temperature of 32°C until the OD value is 1 to obtain a composite fermentation bacterial liquid.

[0091] The almonds are sun-dried and purchased from Chongqing Chinese herbal medicine market.

[0092] Licorice root is a sun-dried product purchased from Chongqing Chinese herbal medicine market.

[0093] The wild chrysanthemum flowers were sun-dried and purchased from Chongqing Hengsheng Chinese Medicine Market.

[0094] The root of Sophora flavescens was sun-dried and purchased from Chongqing Hengsheng Chinese Medicine Market.

[0095] The roots of Artemisia annua L. are sun-dried products and were purchased from the Bund Chinese herbal medicine market in Chongqing.

[0096] Magnolia flowers and Stephania tetrandra were sun-dried products purchased from the Bund Chinese herbal medicine market in Chongqing.

[0097] The preservative is phenoxyethanol, which was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0098] Peptone was purchased from Haibo Biotechnology Co., Ltd.

[0099] Glucose was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0100] Potassium dihydrogen phosphate was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0101] Magnesium sulfate was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0102] Sodium chloride was purchased from Shanghai Tengzhun Biotechnology Co., Ltd.

[0103] Lettuce juice is made from fresh lettuce.

[0104] Kale juice is made by juicing fresh kale.

[0105] Spinach juice is made from fresh spinach juice.

[0106] Pichia mansoni was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D25102.

[0107] Gibberella avenae was purchased from Shanghai Collection Biotechnology Center, strain number: SHBCC D13369.

[0108] Staphylococcus sciuridae was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D17780.

[0109] Comparative Example 1 A method for extracting plant polyphenol antibacterial and anti-allergic agents, compared with Example 2, differs only in that: Bacillus subtilis was used to replace Pichia mansoni in equal amounts.

[0110] Bacillus subtilis was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D11378.

[0111] Comparative Example 2 A method for extracting plant polyphenol antibacterial and anti-allergic agents, compared with Example 2, differs only in that: An equal amount of Bacillus palmitofermentans was used to replace Gibberella avenae.

[0112] Palm fermentation Bacillus was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D51614.

[0113] Comparative Example 3 A method for extracting plant polyphenol antibacterial and anti-allergic agents, compared with Example 2, differs only in that: Lactobacillus fermentum was used to replace Staphylococcus sciurii in equal amounts.

[0114] Lactobacillus fermentum was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D24519.

[0115] Comparative Example 4 A method for extracting plant polyphenol antibacterial and anti-allergic agents, compared with Example 2, differs only in that: Bacillus subtilis was used to replace Pichia mansoni in equal amounts, Bacillus palmitolyticus was used to replace Gibberella avenae in equal amounts, and Lactobacillus fermentum was used to replace Staphylococcus sciuri.

[0116] Bacillus subtilis was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D11378.

[0117] Palm fermentation Bacillus was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D51614.

[0118] Lactobacillus fermentum was purchased from Shanghai Biotechnology Collection Center with the strain number: SHBCC D24519.

[0119] Comparative Example 5 A method for extracting plant polyphenol antibacterial and anti-allergic agents, compared with Example 2, differs only in that: Replace lettuce juice with an equal amount of apple juice, replace kale juice with an equal amount of pear juice, and replace spinach juice with an equal amount of cucumber juice.

[0120] Apple juice is made from freshly squeezed apples.

[0121] Snow pear juice is made from fresh snow pear juice.

[0122] Cucumber juice is made from fresh cucumber juice.

[0123] Experiment 1 The antibacterial properties of the test samples prepared from the plant polyphenol antibacterial and anti-allergic agents of the embodiments and comparative examples were tested using the inhibition zone method-punch method.

[0124] The plate culture medium used was: peptone 10 g / L, beef extract 10 g / L, yeast powder 5 g / L, glucose 20 g / L, NaCl 10 g / L, agar powder 20 g / L, pH 7.5, and the solvent was distilled water.

[0125] The diameter of the culture medium hole is 6 mm.

[0126] Propionibacterium acnes was spread on the culture medium.

[0127] Inject the sample to be tested into the wells of the culture medium, filling the wells without overflowing.

[0128] The cells were cultured at 37°C for 24 h, and the diameter of the inhibition zone was measured.

[0129] Test sample: 10g plant polyphenol antibacterial and anti-allergic agent, 90g deionized water, mix well.

[0130] Experiment 2 According to T / JSRH 003-2023 "Hyaluronidase Inhibition Method for Anti-allergic Activity Test of Cosmetic Raw Materials", the hyaluronidase inhibition rate (%) of the test samples prepared from the plant polyphenol antibacterial and anti-allergic agents of each embodiment and comparative example was tested.

[0131] Test sample: 10g plant polyphenol antibacterial and anti-allergic agent, 90g deionized water, mix well.

[0132] The specific experimental data of Experiments 1-2 are shown in Table 1.

[0133] Table 1 According to the data comparison of each embodiment and comparative example in Table 1, the antibacterial performance and soothing performance of each embodiment are significantly better than those of each comparative example. It can be seen that the bacterial liquid obtained by culturing Pichia mansoni, Gibberella avenae, and Staphylococcus sciuri in a special liquid culture medium in each embodiment to ferment the composite plant can have a more significant antibacterial and anti-allergic effect, can repair acne faster, and well meet the needs of consumers.

[0134] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make non-creative modifications to the present embodiment as needed, but as long as they are within the scope of the claims of the present application, they are protected by the patent law.

Claims

1. A method for extracting plant polyphenol antibacterial and anti-allergic agents, characterized by: The following steps are involved: Step 1) crushing and mixing apricot kernel, liquorice root, wild chrysanthemum, sophora flavescens root, artemisia annua root, magnolia flower, and stephania tetrandra to obtain a composite plant; Step 2), mixing the composite plant with water, heating and extracting, to obtain a pre-extract; Step 3), after cooling the pre-extract, adding the composite fermentation bacterial solution and fermenting for 20-22 days to obtain a fermentation complex; Step 4), filtering and sterilizing the fermentation complex to obtain a fermentation broth; Step 5), concentrating and drying the fermentation liquid to obtain a composite plant extract; Step 6), mixing the composite plant extract, water, and preservative to obtain a plant polyphenol antibacterial and anti-allergic agent; The composite fermentation bacterial liquid is prepared by compounding Pichia mansoni, Gibberella avenae, Staphylococcus squirrel and liquid culture medium; The OD value of the composite fermentation bacterial liquid is ≥1.

2. The method for extracting a plant polyphenol antibacterial and anti-allergic agent according to claim 1, wherein: In the step 1), the mass ratio of almond, liquorice root, wild chrysanthemum, sophora flavescens root, artemisia annua root, magnolia flower and stephania tetrandra is 2.5-3.5:2.5-3.5:1.5-2.5:1.5-2.5:1.5-2.5:1.5-2.5:0.5-1.

5.

3. The method for extracting a plant polyphenol antibacterial and anti-allergic agent according to claim 2, wherein: In the step 2), the mass ratio of the composite plant to water is 1:5-6.

4. The method for extracting a plant polyphenol antibacterial and anti-allergic agent according to claim 1, wherein: In the step 2), the mixture is heated to 80-85° C. and extracted for 6-8 hours.

5. The method for extracting a plant polyphenol antibacterial and anti-allergic agent according to claim 1, wherein: In the step 3), the temperature is kept at 30-32° C. and the fermentation is carried out for 20-22 days.

6. The method for extracting a plant polyphenol antibacterial and anti-allergic agent according to claim 1, characterized in that: In step 6), the mass ratio of the composite plant extract, water, and preservative is 1.725-2.78:12.12-13.2:0.075-0.

1.

7. A method for extracting a plant polyphenol antibacterial and anti-allergic agent according to any one of claims 1 to 6, characterized in that: In the composite fermentation bacterial liquid, the mass ratio of Pichia mansoni, Gibberella avenae, Staphylococcus sciuri and liquid culture medium is 1-2:3-4:2-3:1000.

8. The method for extracting a plant polyphenol antibacterial and anti-allergic agent according to claim 7, characterized in that: The liquid culture medium is a compound of water, peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, sodium chloride, lettuce juice, kale juice, and spinach juice; the mass ratios of water, peptone, glucose, potassium dihydrogen phosphate, magnesium sulfate, sodium chloride, lettuce juice, kale juice, and spinach juice in the liquid culture medium are 18-22:18-22:1.8-2.2:0.8-1.2:4-6:50-60:50-60:50-60:766.6-807.

4.

9. The method for extracting a plant polyphenol antibacterial and anti-allergic agent according to claim 8, characterized in that: The mass ratio of the composite plant to the composite fermentation bacteria liquid is 10:1-2.

10. A plant polyphenol antibacterial and anti-allergic agent, characterized in that: The invention is prepared by the extraction method of the plant polyphenol antibacterial and anti-allergic agent according to any one of claims 1 to 9.

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