66 results about "Chitosan nanoparticles" patented technology

The invention relates to the technical field of pharmaceutical preparations, and discloses a composition with liver protection and antioxidation effects and a preparation method thereof.The composition is prepared from galactosamine modified astragalus membranaceus exosome, schisandra chinensis nanocrystals, liquorice-chitosan nanoparticles, lactobacillus acidophilus freeze-dried powder, beta-(1, 3) / (1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate) sourced from saccharomyces cerevisiae, and beta-(1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate). And (6) a dextran, grape exosome-curcumin hybrid vesicle freeze-dried powder, a curcumin-mitochondrial targeting peptide compound, a resveratrol-hydroxypropyl-beta-cyclodextrin inclusion compound and reduced panthenol. According to the liver-protecting and anti-oxidation composition disclosed by the invention, multi-dimensional intervention on liver protection is realized through an elaborately designed ternary system. The system is composed of a traditional Chinese medicine extract, a microorganism-plant exosome combination and a high-bioavailability antioxidant, all the parts have a synergistic effect, and the liver protection effect is remarkably improved.

The invention provides a chitosan-iron nanoparticle leaf surface resistance and control agent as well as a preparation method and application thereof, and belongs to the technical field of reduction of heavy metal pollution of crops. The preparation method comprises the following steps: dissolving chitosan in an organic solvent to obtain a chitosan solution, mixing the chitosan solution with ferrous sulfate, adding a sodium tripolyphosphate solution for crosslinking and stirring to obtain a solution containing precipitates, centrifuging, and collecting the precipitates to obtain the chitosan / ferrous sulfate composite material. The TPP and amino groups of the chitosan form ionic crosslinking through triphosphate groups, and Fe < 2 + > is successfully loaded on the surfaces of the chitosan nanoparticles. By complexing chitosan and Fe < 2 + >, the crystallinity of the material is reduced, and the adsorption activity of the amorphous structure is enhanced. Pot experiments show that by spraying the chitosan-iron nanoparticle leaf surface resistance and control agent on leaf surfaces, heavy metals of crops can be remarkably reduced, the antioxidant enzyme activity of plant tissues is improved, active oxygen is effectively removed, oxidative damage is relieved, and therefore plant growth is promoted, and the toxic effect of heavy metal stress on the crops is relieved.

The invention relates to a preparation technology of an active bio-based food preservative film with high barrier property and high antibacterial rate, in particular to the field of food packaging and food preservation. The method comprises the following steps: by taking chitosan as a matrix, firstly, loading gallic acid by using nanoparticles prepared from the chitosan and sodium tripolyphosphate; and then, adding the chitosan nanoparticles loaded with gallic acid into a chitosan polymer network to prepare a chitosan-nanoparticle composite film, and preparing the film with high flexibility, high barrier property and high antibacterial activity. The aquatic product preservative has excellent mechanical property, barrier property, biological activity and biodegradability, can effectively prolong the shelf life of aquatic products, and has wide application prospects in the fields of food industry, preservation and the like.

This invention belongs to the field of curcumin-loaded carrier technology, and discloses a modified chitosan carrier, its preparation and application, and modified chitosan nanoparticles loaded with curcumin. Preparation method: Under magnetic stirring in an ice-water bath, a modifier is added dropwise to a chitosan solution, and the reaction is carried out at room temperature with stirring to obtain a product liquid. This product liquid is mixed with glacial acetic acid, dialyzed, and filtered to obtain a clear and transparent solution. When the modifier is an aqueous solution of trimethylammonium chloride, the clear and transparent solution is concentrated by rotary evaporation and freeze-dried to obtain the modified chitosan carrier. When the modifier is an aqueous solution of acrylamide, the clear and transparent solution is concentrated by rotary evaporation and hydrolyzed to obtain a hydrolysate, which is mixed with glacial acetic acid, dialyzed, and freeze-dried to obtain the modified chitosan carrier. This invention significantly improves the encapsulation efficiency, drug loading, and delivery stability of curcumin in the modified chitosan nanoparticle carrier prepared by this invention.

The invention relates to the technical field of biopesticides, and provides a slow-release insecticide for landscaping and a preparation method thereof.The slow-release insecticide is prepared from, by weight, 5%-20% of clothianidin, 1%-5% of cyromazine, 1.5%-2.5% of metalaxyl hymexazol, 0.8%-1.2% of humic acid, 5%-8% of chitosan, 3%-4% of polylactic acid, 1.5%-2.5% of nanometer titania, 0.5%-0.8% of plant source auxiliaries and the balance water; compared with existing insecticides, a'stepped release 'system (clothianidin rapidly controls insects in the early stage and cyromazine is long-acting and stable in the later stage) is formed through a nano slow release technology (chitosan nanoparticles and PLA microspheres), synergistic prevention and control of overground and underground insects are effectively achieved, the instantaneous concentration of the insecticide in the environment is reduced, and the resistance selection pressure is reduced.

The invention provides a preparation method and equipment of a composition helpful for controlling carbon water absorption. According to the preparation method, the phyllanthus emblica, the sugarcane polyphenol, the white kidney bean extract, the mulberry leaf extract and the L-arabinose are respectively embedded by adopting chitosan nanoparticles, so that the stability of active ingredients is improved, and fixed-point release of intestinal tracts is realized. And uniformly mixing the obtained embedded particles, protein powder, dietary fibers and other raw materials through a three-dimensional mixing machine, and carrying out composite molding on the obtained mixture and a sugar-oil mixture to prepare the high-protein meal replacement bar which plays a role in synergistically controlling sugar and promoting lipid metabolism. According to the preparation equipment, an auxiliary mixing assembly is additionally arranged in a mixing barrel and comprises a guide bearing rod distributed along a barrel shaft and an auxiliary mixing part capable of sliding along the guide bearing rod, the mixing barrel moves to drive the auxiliary mixing part, and meanwhile reciprocating sliding is achieved through the gravity effect. According to the structure, the powder overturning and dispersing effects are enhanced, agglomeration is effectively broken, the mixing uniformity and stability are improved, and the mixing efficiency is remarkably improved under the condition of low energy consumption.

This application relates to an anti-icing coating, its preparation method, and its application, belonging to the field of protective coating technology. The anti-icing coating comprises a superhydrophobic self-cleaning coating and a hydrophobic and oleophobic layer stacked together. The superhydrophobic self-cleaning coating includes first SiO2 nanoparticles and fluorosilane. The hydrophobic and oleophobic layer includes second SiO2 nanoparticles, nanocellulose, chitosan nanoparticles, and a binder. The use of natural polymer materials such as nanocellulose and chitosan nanoparticles in the hydrophobic and oleophobic layer results in lower cost and better environmental friendliness. The superhydrophobic self-cleaning coating achieves superhydrophobic properties through the synergistic effect of a nano-dispersion system and a "papillary" micro-nano rough structure. The hydrophobic and oleophobic layer further enhances the coating's hydrophobic and oleophobic properties and surface lubricity, significantly reducing the adhesion of ice to the coating surface.

This invention is related to the fields of medical biotechnology, nanobiotechnology, and nanomedicine. It involves a nanomedicine product that can release HOC1 (hypochlorous acid) at the tumor site in a controlled manner to treat lung cancer. HOC1 is a potent antimicrobial and antitumor agent that can target tumor tissues while minimizing damage to healthy tissues. The produced nanoparticles have a two-layer structure: the inner layer contains alginate, PFP (perfluoropentane), and Ca(OCl)2 to release HOC1, and the outer layer has a chitosan coating with folate and salicylic acid for targeting and proton release. The production process involves nanoemulsion and coating with the ionic gelation technique. When the nanoparticles are delivered to the lungs and targeted to cancer cells with folate, ultrasound radiation triggers the release of HOC1 to induce apoptosis and destroy cancer cells effectively, reducing side effects and improving treatment outcomes for lung cancer patients.

The invention provides a drug delivery system, the drug delivery system is formed by cross-linking and curing hyaluronic acid conductive hydrogel and chitosan nanoparticles modified by chlorotoxin, the hyaluronic acid conductive hydrogel is formed by cross-linking and curing hyaluronic acid-methacrylic anhydride and reduced graphene oxide, and the chitosan nanoparticles modified by chlorotoxin are formed by cross-linking and curing hyaluronic acid-methacrylic anhydride and reduced graphene oxide. The chlorotoxin-modified chitosan nanoparticles are formed by loading a therapeutic drug on chitosan nanoparticles, coupling chlorotoxin by adopting an EDC / NHS activation method, and modifying by PEG (Polyethylene Glycol). The invention also provides a preparation method of the drug delivery system. The invention also provides application of the drug delivery system in preparation of drugs for treating tumors. According to the invention, two stages of carriers are used for synergistically and doubly loading therapeutic drugs, so that the conductive function, biocompatibility, drug loading stability and release controllability are considered, and the MGMT high-expression mediated tumor drug resistance is effectively reversed.

The invention relates to an anti-inflammatory plasma matrix as well as a preparation method and application thereof. The anti-inflammatory plasma matrix comprises a plasma matrix and aspirin chitosan nanoparticles, the preparation method of the anti-inflammatory plasma matrix comprises the step of pressing a mixture of the plasma matrix and the aspirin chitosan nanoparticles to obtain the anti-inflammatory plasma matrix. Leukocyte contained in the plasma matrix in the anti-inflammatory plasma matrix can play an antibacterial role, aspirin can be slowly released by the aspirin chitosan nano-particles, the function of regulating and controlling bone regenerative cycle in an inflammatory state is achieved, and a chitosan shell also has certain antibacterial and anti-inflammatory effects. The prepared anti-inflammatory plasma matrix can be applied to preparation of drugs for treating periodontitis and peri-implantitis.

The present invention relates to nucleic acid fragment-encapsulated polymer nanoparticles and a method of preparing the same. The polymer may be a mucoadhesive polymer and specifically chitosan, and the nucleic acid fragment may be polydeoxyribonucleotide (PDRN). The nucleic acid fragment-encapsulated polymer nanoparticles may be used as an eye drop. Since the nucleic acid fragment-encapsulated polymer nanoparticles is capable of adhering to the mucus in the eyes, nucleic acid fragments inside the nanoparticles can be slowly released into the ocular mucous membrane. In addition, since nucleic acid fragment-encapsulated polymer nanoparticles can exhibit high efficiency in the eyes even with a small dose of drug, patient convenience can be increased or costs can be reduced.

The invention belongs to the technical field of functional biopolymer materials, and relates to alkyl alkenyl succinic anhydride modified chitosan nanoparticles as well as a preparation method and application thereof. The nano-particles are formed by self-assembling after chitosan is subjected to hydrophobic modification through alkyl alkenyl succinic anhydride and the pH of a system is adjusted under the condition from acidity to neutrality or alkalescence. The degree of substitution of the nanoparticles is 1-40%. The invention also provides a preparation method and application of the nanoparticles. The nanoparticles are suitable for various alkyl alkenyl succinic anhydrides with the carbon chain length of C6-C16, and a new technical scheme is provided for constructing an oil-water dispersion system stabilized by bio-based particles.

The present invention, based on a non-bubble-based sonoporation technique, provides an ultrasound-assisted delivery platform for drug-loaded chitosan nanoparticles. This platform comprises: the administration of drug-loaded chitosan nanoparticles and the application of ultrasound stimulation. The drug-loaded chitosan nanoparticles are delivered to a target site, where ultrasound waves are directly applied to induce cellular membrane deformation. The ultrasound mechanical force temporarily alters membrane permeability, thereby facilitating the intracellular delivery of the drug-loaded chitosan nanoparticles.

The invention relates to the field of antifouling coatings, and particularly discloses a green environment-friendly water-based industrial antifouling coating and a preparation method thereof. The antifouling paint comprises 40-55% of bio-based FEVE resin, 8-15% of a bio-based functional additive, 5-8% of nano titanium dioxide, 8-12% of wollastonite powder, 5-7% of calcium carbonate, 3.0-4.5% of other auxiliaries and the balance of water, wherein the bio-based functional additive comprises palm oil fatty glyceride, chitosan nanoparticles and a bamboo fiber / nano cellulose compound. According to the invention, the vegetable oil modified FEVE resin is used as a core raw material to replace a traditional petroleum-based raw material, and is matched with a relatively small addition amount of the bio-based functional additive and fillers such as nano titanium dioxide, wollastonite powder and calcium carbonate, so that the antifouling paint with environmental friendliness, hydrophobicity, stain resistance and weather resistance is prepared, the purpose of long-acting antifouling is achieved, and the environment-friendly, hydrophobic and weather-resistant effects are achieved. Moreover, the production cost is lower, and the application prospect is very wide.

The application belongs to the technical field of medicine, and particularly relates to a composition for alleviating and repairing testicular damage caused by heat stress and application thereof. The composition for alleviating and repairing testicular damage caused by heat stress comprises the following raw materials: lycopene inclusion compound, ginsenoside Re, modified chitosan nanoparticles and a solvent. The amount of the modified chitosan nanoparticles is 10-15 wt% of the ginsenoside Re. The application innovatively designs a combination formula with lycopene and ginsenoside Re as the core. Both of the two main effective components in the formula are natural plant or traditional Chinese medicine extract, which effectively avoids the conflict between the medicinal properties of traditional Chinese medicines and has good biological safety. The composition can significantly repair the testicular damage caused by heat stress and accelerate the recovery after heat stress damage.

The present invention provides compositions and methods for detecting tumor tissues in a subject. Embodiments of the present invention provide biocompatible chitosan nanoparticles suitable for dual modality optical imaging. Embodiments of the present invention provide methods for fabricating chitosan nanoparticles. Embodiments of the present invention provide methods for detecting tumor tissues in a subject using compositions provided by the present invention.

The invention belongs to the technical field of biological medicines, and particularly discloses a nano drug-loaded catheter coating material based on TUBA1C gene expression regulation and a preparation method thereof. The coating material is composed of siRNA (small interfering ribonucleic acid) loaded with TUBA1C and PLGA (poly (lactic-co-glycolic acid)) / chitosan nanoparticles loaded with antibacterial peptide GL13K. The sequences of the siRNA are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. Nanoparticles are prepared through a multiple emulsion-solvent evaporation method, and the surface of the catheter is coated with the nanoparticles. The coating provided by the invention can synergistically exert gene silencing and antibacterial effects, and siRNA silences a TUBA1C gene in urethral mucosa cells in a targeted manner and inhibits inflammatory response and fibrosis; the antibacterial peptide GL13K can kill pathogenic bacteria and prevent the formation of biological membranes. The coating material has antibacterial, anti-inflammatory and anti-biofilm functions, can effectively prevent and treat catheter-related urinary tract infection, and reduces the risk of long-term complications such as urethrostenosis.

The invention discloses a soluble microneedle array transdermal drug delivery freckle-removing mask and a preparation method of the soluble microneedle array transdermal drug delivery freckle-removing mask. The mask comprises a backing layer and a soluble micro-needle array arranged on the backing layer, the micro-needle array is composed of a needle tip and a base, and the needle tip comprises a freckle-removing active component, a nano-carrier material, a micro-needle matrix material and a stabilizer. The freckle-removing active components comprise tranexamic acid, sodium ascorbyl phosphate, Maojian green tea extract, licoflavone and nicotinamide; the nano carrier material is selected from hydroxyl cyclodextrin, chitosan nanoparticles and alginic acid-chitosan nanoparticles; the microneedle matrix material comprises hyaluronic acid, polyvinylpyrrolidone and polyvinyl alcohol. Through combination of a nano-carrier technology and a micro-needle system, the drug loading capacity, stability and transdermal efficiency of freckle-removing active ingredients are remarkably improved, and the freckle-removing micro-needle has the advantages of being good in freckle-removing effect, painless, noninvasive, convenient to use and the like, and is suitable for prevention and treatment of chloasma, freckles, hyperpigmentation after inflammation and other pigmented skin diseases.

The invention provides a drug-loaded intravascular stent and a preparation method thereof.The preparation method of the drug-loaded intravascular stent comprises the steps that drug-loaded microspheres are prepared; wherein the drug-loaded microspheres comprise chitosan nanoparticles loaded with an anti-inflammatory drug, an antibacterial drug layer coating the chitosan nanoparticles, and a PLGA (poly (lactic-co-glycolic acid)) shell layer coating the antibacterial drug layer; mixing the drug-loaded microspheres with PLGA (poly (lactic-co-glycolic acid)) microspheres to prepare a blended wire rod; and according to a digital model, carrying out 3D printing on the blended wire rod to form the drug-loaded intravascular stent.

The invention discloses galactosyl fluorinated chitosan-EGCG (epigallocatechin gallate) nanoparticles and application thereof, and belongs to the field of steady-state delivery of functional foods. The preparation method comprises the following steps: firstly, synthesizing galactosyl modified fluorinated chitosan by utilizing an amidation reaction between lactobionic acid carboxyl and fluorinated chitosan amino, and successfully preparing the tea polyphenol EGCG-loaded nano steady-state delivery carrier through an ionic gel method. The carrier can utilize the fluorine effect of the fluorinated group on the surface of the carrier and the interaction of the galactose-SGLT1 receptor to enhance the intestinal targeted delivery of EGCG (epigallocatechin gallate). The preparation method is simple to operate, the prepared galactosyl-modified fluorinated chitosan nanoparticle delivery carrier has high loading rate on EGCG (epigallocatechin gallate), the bioavailability of EGCG can be improved, and the galactosyl-modified fluorinated chitosan nanoparticle delivery carrier is of great significance in promoting development and application of functional food.

The invention discloses a novel anti-salmonella nano preparation CCJY as well as a preparation method and application thereof. The preparation comprises chitosan nano particles. The chitosan nanoparticle comprises cross-linked chitosan, and a lasso peptide MccJ25 and a lasso peptide MccY which are loaded on the chitosan. According to the invention, the synergistic antibacterial effect among different lasso peptides is exerted through a chemical cross-linking technology, on the basis of integrating the original antibacterial types of the lasso peptide MccJ25 and the lasso peptide MccY, the antibacterial types are expanded to serratia marcescens and enterococcus cloacae, and the broad-spectrum antibacterial effect is realized. Meanwhile, the invention further discloses a preparation method and application of the chitosan nano-particles and an anti-salmonella nano-preparation containing the chitosan nano-particles.

This application discloses a composite aerogel for controlling Nisin release, its preparation method, and its application, relating to the field of food engineering technology. By selecting chitosan nanoparticles and nanocellulose as two loading materials, Nisin is dual-loaded to improve the Nisin loading rate. The composite aerogel obtained by freeze-drying has a three-dimensional porous network structure inside, which can provide channels for Nisin delivery and release, prolong the Nisin release cycle, and realize the sustained and controlled release of Nisin.

The application belongs to the field of marine biotechnology, and particularly relates to natural polysaccharide-quaternized chitosan nanoparticles with immune stimulation and preparation and application thereof. The nanoparticles formed by natural polysaccharide with negative charge and quaternized chitosan with positive charge are obtained by polyelectrolyte complexation, the particle size is 316.4 nm, and the potential is 31.1 mV; the quaternized chitosan with positive charge and the natural polysaccharide with negative charge are mixed in a mass ratio of 6.25-10:1-4. After immunization of mice, the secretion amount of immunoglobulin IgG and immune factors in the mice is determined, which shows that the nanoparticles have good immune activity, thereby playing an effect in vaccine adjuvant. The application provides a method and guidance for the research on natural polysaccharide and chitosan derivatives as immune adjuvants in recent years.

The invention relates to the field of biotechnology, medicine and cosmetology, and more particularly to a hybrid matrix for use as a biological graft in the preparation of cardiac prostheses. The technical result of the invention includes improving the surface biocompatibility and the stability and durability of a biological graft which provides an antimicrobial response, and also preventing calcification. A method for producing a hybrid biomatrix comprises the following steps: pre-purifying a base of a biomatrix for 0.75-6 hours in two stages, consisting in the decellularization of xenopericardium in a 2-5 wt% water-alcohol solution of Tween-80, saturated with CO2, at a temperature of 20-400°C and a pressure of 10-30 MPa, and the subsequent decellularization of the xenopericardium in a multi-component system containing a solution based on supercritical CO2 and a 1-1.5 vol% addition of a 2-5 wt% water-alcohol solution of Tween-80, at a temperature of 20-400°C and a pressure of 10-30 MPa; stabilizing the biomatrix base by treatment with a 0.1-0.5 wt% aqueous buffer solution of genipin having a pH of 6.0-7.4 for 3-4 hours; and applying to the pre-purified biomatrix base a coating consisting of an aqueous solution of chitosan nanoparticles with an antimicrobial doping additive in the form of silver or vancomycin nanoparticles in a carbonic acid solution, at a pressure of 20-30 MPa and a temperature of 23-270°C for 1-6 hours.

Disclosed herein are compositions comprising chitosan and at least 1% (w / v) nitrogen. In some cases, at least 50% of the nitrogen is in the chemical form of amine and / or nitrate and / or ammonia. Also disclosed herein are methods of producing chitosan nanoparticles by dissolving chitosan in acidic water, wherein the water is acidic by an acid selected from the group consisting of acetic acid, nitric acid, sulfuric acid, hydrochloric acid, and combinations thereof; and forming nanoparticles from the dissolved chitosan. Further disclosed is the treatment of plants or plant parts using the compositions disclosed herein.

The application discloses a quercetin-chitosan nanoparticle suspension eye drop, a preparation method and application thereof. The eye drop contains the following components on the basis of 100ml of preparation: quercetin 0.001-0.006g; chitosan 0.05-0.3g; sodium tripolyphosphate 0.01-0.05g; sodium chloride 0.6-1.5g; benzalkonium bromide 0.005-0.02g; borate buffer 1-3g; and the rest is injection water, and the total amount is 100ml. Quercetin is loaded in nanoparticles formed by ion cross-linking of chitosan and sodium tripolyphosphate. The application solves the technical defects of poor water solubility and easy oxidation of quercetin by constructing a polyphenol-chitosan nanocomposite system. The application has a dual synergistic mechanism of ROS clearance and mucosa repair, significantly improves the ocular bioavailability of quercetin, and has a wide application prospect in drugs for preventing or treating dry eye.

The invention discloses an oral composition containing polysaccharide-based cinnamon oil antibacterial Pickering emulsion and a preparation method thereof, and belongs to the technical field of oral care products. The composition comprises Pickering emulsion and an orally acceptable carrier, wherein the Pickering emulsion takes cinnamon oil as a dispersion phase and polysaccharide-based solid particles as a stable phase, and the content of the Pickering emulsion is 20-60%; the polysaccharide-based solid particles are preferably chitosan nano-particles of which the surfaces are modified in situ by polydopamine, the emulsion is prepared by high-speed shearing and emulsifying, and the composition can be prepared into toothpaste and mouth wash. The Pickering emulsion is constructed through polysaccharide-based particles, the problem that cinnamon oil is easy to volatilize and degrade is solved, polydopamine in-situ modification endows the emulsion with excellent oral adhesion and retention capacity, saliva scouring and pH fluctuation can be resisted, and the long-acting effect of the cinnamon oil is achieved; meanwhile, the synergistic antibacterial and anti-biofilm effect of the polysaccharide-based particles, the cinnamon oil and the polydopamine is achieved, the preparation process is simple, the method is matched with an existing oral care product production system, and safety and industrialization feasibility are excellent.

System for the development of a pH-based biosensor using anthocyanins from red cabbage (Brassica oleracea) and chitosan nanoparticles for monitoring food quality, comprising: ◯ Glassware for collecting and processing red cabbage samples, using a system for extracting anthocyanins by: • Thoroughly wash red cabbage with tap water and distilled water; • Chop the washed cabbage into fine pieces of about 1-2 cm; • Soaking 2 kg of shredded cabbage in 100 ml of acidified ethanol solution (ethanol:water:acetic acid, 70:29:1 v / v / v) for 24 hours; • Filter the extract using a vacuum filter and concentrate it under reduced pressure; • Store the extract at 4°C in a dark bottle; ◯ a device for the synthesis of chitosan nanoparticles via ionic gelation, comprising: • Prepare a 0.1% (w / v) chitosan solution in 1% acetic acid, adjusted to pH 4.6; • Add 0.1% tripolyphosphate (TPP) solution dropwise while stirring; • Collecting the nanoparticles by centrifugation and washing with deionized water; ◯ a system for introducing anthocyanins into chitosan nanoparticles by mixing the anthocyanin extract with the chitosan solution prior to TPP addition; ◯ a solution casting system for the production of a pH-sensitive biofilm by mixing anthocyanin-loaded nanoparticles with poly(vinyl alcohol); ◯ analytical instruments, including an Alpha E ATR-FTIR (Bruker, Germany) and an FEI QUANTA 450 scanning electron microscope, for characterizing the nanoparticles and biofilm; ◯ Design of a well diffusion test to assess antibacterial activity against Staphylococcus aureus using Mueller-Hinton agar plates.

The invention discloses a compound fermented wine beverage and a preparation method thereof, and belongs to the technical field of functional beverage processing. The preparation method comprises the following steps: by taking radix angelicae, rhizoma polygonati, radix puerariae and ganoderma lucidum spore powder as mixed raw materials, carrying out superfine grinding, then adding chitosan nanoparticles, adding water, and uniformly mixing, so as to obtain a pretreated mixed solution; performing ultrasonic treatment on the pretreated mixed solution, performing first-stage enzymolysis by using a compound enzyme of cellulase and xylanase, performing second-stage enzymolysis by using a compound enzyme of pectinase and beta-glucosidase, and performing sugar degree adjustment after the second-stage enzymolysis to obtain an enzymolysis mixed solution; adding a fermentation strain into the enzymolysis mixed solution, sequentially carrying out aerobic fermentation, transition fermentation, anaerobic fermentation and pulse type curing, and adjusting the alcoholic strength to obtain a fermentation mixed solution; and filtering, sterilizing and filling the fermented mixed solution to obtain the compound fermented wine beverage. The compound fermented wine beverage can solve the problems that traditional fermented wine is low in active ingredient content, single in function and poor in taste and stability, and is wide in application prospect.

The application provides an RU.521-EVs anti-radiation nanogel and a preparation method and application thereof. The RU.521-EVs anti-radiation nanogel is obtained by loading microalgal-derived extracellular vesicles and a small molecule compound RU.521 on chitosan nanoparticles formed by cross-linking of chitosan. The RU.521-EVs anti-radiation nanogel has stronger adhesion and permeability in the lung and can effectively release RU.521 and EVs, and the RU.521 and EVs can synergistically exert anti-inflammatory and antioxidant effects, effectively start the local antioxidant and anti-inflammatory defense response of the lung, and exhibit excellent anti-radiation effect, and can be used for preventing and treating radiation-induced lung injury.