100 results about "Chitosan nanoparticles" patented technology

The invention relates to the technical field of pharmaceutical preparations, and discloses a composition with liver protection and antioxidation effects and a preparation method thereof.The composition is prepared from galactosamine modified astragalus membranaceus exosome, schisandra chinensis nanocrystals, liquorice-chitosan nanoparticles, lactobacillus acidophilus freeze-dried powder, beta-(1, 3) / (1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate) sourced from saccharomyces cerevisiae, and beta-(1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate). And (6) a dextran, grape exosome-curcumin hybrid vesicle freeze-dried powder, a curcumin-mitochondrial targeting peptide compound, a resveratrol-hydroxypropyl-beta-cyclodextrin inclusion compound and reduced panthenol. According to the liver-protecting and anti-oxidation composition disclosed by the invention, multi-dimensional intervention on liver protection is realized through an elaborately designed ternary system. The system is composed of a traditional Chinese medicine extract, a microorganism-plant exosome combination and a high-bioavailability antioxidant, all the parts have a synergistic effect, and the liver protection effect is remarkably improved.

The invention discloses a preparation method and application of active cationic chitosan nanoparticles, which comprises the following steps: reacting a low-molecular chitosan triethylamine hydrogen sulfate ionic liquid ([(C2H5) 3NH] HSO4) solution with an aldehyde compound ethanol solution to form Schiff base protection on chitosan C2-site amino, and then reacting with 2, 3, 5, 6-tetramethyl-4-piperidine-2-one to obtain the active cationic chitosan nanoparticles. The preparation method comprises the following steps: carrying out nucleophilic substitution reaction on chitosan C6 in the presence of a basic catalyst by using 2, 3-epoxypropyl trimethyl ammonium chloride, then adding a chemical initiator, carrying out grafting reaction on chitosan C3 and acrylic acid, and then removing Schiff base protection on chitosan C2. Carboxylate anions of acrylic acid and cations of chitosan are subjected to cross-linking self-assembly through a hydrothermal reaction, and the chitosan nanoparticles which are high in stability, small in particle size, uniform in distribution and efficient and lasting in function are obtained. The prepared nanoparticles are high in reaction activity, contain a large amount of quaternary ammonium salt, amino dication groups and carboxyl groups, and have wide application prospects in the fields of dye adsorption, heavy metal adsorption, textile function finishing and the like.

The invention provides a chitosan-iron nanoparticle leaf surface resistance and control agent as well as a preparation method and application thereof, and belongs to the technical field of reduction of heavy metal pollution of crops. The preparation method comprises the following steps: dissolving chitosan in an organic solvent to obtain a chitosan solution, mixing the chitosan solution with ferrous sulfate, adding a sodium tripolyphosphate solution for crosslinking and stirring to obtain a solution containing precipitates, centrifuging, and collecting the precipitates to obtain the chitosan / ferrous sulfate composite material. The TPP and amino groups of the chitosan form ionic crosslinking through triphosphate groups, and Fe < 2 + > is successfully loaded on the surfaces of the chitosan nanoparticles. By complexing chitosan and Fe < 2 + >, the crystallinity of the material is reduced, and the adsorption activity of the amorphous structure is enhanced. Pot experiments show that by spraying the chitosan-iron nanoparticle leaf surface resistance and control agent on leaf surfaces, heavy metals of crops can be remarkably reduced, the antioxidant enzyme activity of plant tissues is improved, active oxygen is effectively removed, oxidative damage is relieved, and therefore plant growth is promoted, and the toxic effect of heavy metal stress on the crops is relieved.

The invention provides RU.521-EVs anti-radiation nanogel as well as a preparation method and application of the RU.521-EVs anti-radiation nanogel. The RU.521-EVs anti-radiation nanogel is a nanogel obtained by loading microalgae-derived extracellular vesicles and a small molecule compound RU.521 on chitosan nanoparticles formed by cross-linking chitosan, and the extracellular vesicles and the small molecule compound RU.521 are derived from microalgae. The RU.521-EVs anti-radiation nanogel has higher adhesion and permeability in the lung and effectively releases RU.521 and EVs, the RU.521 and EVs can synergistically play anti-inflammatory and anti-oxidation roles, local anti-oxidation and anti-inflammatory defense reactions of the lung are effectively started, the excellent anti-radiation effect is shown, and the RU.521-EVs anti-radiation nanogel can be used for preventing and treating radiation-induced lung injury.

The invention belongs to the technical field of biological medicines, discloses a preparation method of a pcDNA3.1-GH-loaded chitosan nanoparticle gene delivery system and an application of the pcDNA3.1-GH-loaded chitosan nanoparticle gene delivery system in fishes, and aims to solve the problems that the existing gene delivery technology is complicated in operation, the public are not trusted to transgenic foods and the growth rate of the fishes is low. The invention provides an oral chitosan nano-gene delivery system, which is characterized in that pcDNA3.1-GH-loaded chitosan nanoparticles are constructed by compounding a lateolabrax japonicus growth key gene gh with chitosan. Results show that the gene-loaded chitosan nanoparticles can effectively enter cells and realize the expression of exogenous genes, almost have no toxic effect, can achieve an instantaneous transgenosis effect without modifying the genome of an organism, and can significantly improve the growth performance of cultured fishes at the same time. The method has high efficiency, safety and economical efficiency, and provides a reproducible solution for industrialization of an aquaculture sex control technology.

The invention discloses skin antibacterial powder and a preparation method thereof, and relates to the technical field of skin antibacterial products. Comprising the following components in parts by weight: 1-3 parts of chlorhexidine acetate nanocrystals, 5-8 parts of radix sophorae flavescentis powder, 6-10 parts of cortex phellodendri powder, 3-6 parts of cortex dictamni powder, 2-4 parts of sail powder, 1-3 parts of tea polyphenol / chitosan nanoparticles, 15-25 parts of other Chinese herbal medicine extracts, 0.8-1.5 parts of borneol powder and 0.6-1 part of plant polysaccharide. The skin antibacterial powder is good in antibacterial activity, good in antibacterial durability, low in toxic and side effects and remarkable in wound repairing effect.

The invention relates to the technical field of medical preparations, and particularly discloses a selenium-based anti-postmenopausal osteoporosis medicine and a preparation method and application thereof.The selenium-based anti-postmenopausal osteoporosis medicine comprises a main active component, a secondary active component and an auxiliary active component, the main active component is a nano-selenium-phytoestrogen compound and is formed by covalent coupling of selenomethionine and soy isoflavone, the particle size of the main active component is 50-200 nm, and the particle size of the auxiliary active component is 100-200 nm; the mass of the selenium element accounts for 5-30% of the mass of the compound; the auxiliary active component is a collagen peptide-calcium chelate and is prepared from marine collagen peptide and calcium citrate through chelation reaction, and the mass ratio of the calcium element to the collagen peptide is (1: 3)-(1: 5); the targeted delivery carrier is folic acid modified chitosan nanoparticles and is used for loading the main active component and the auxiliary active component; the auxiliary materials comprise a disintegrating agent, a lubricating agent and a pH-sensitive enteric coating material; through the synergistic effect of the main active ingredient nano-selenium-phytoestrogen compound and the auxiliary active ingredient collagen peptide-calcium chelate, bone formation can be promoted and bone resorption can be inhibited at the same time, and dual-function treatment is achieved.

The present invention discloses a chitosan-starch composite Janus particle and its preparation method and application. The present invention uses starch modified with succinic anhydride as a particle stabilizer for Pickering emulsion, and saturated fatty acid as an oil phase to prepare a Pickering emulsion template, so that a part of the starch particles are locked in the solidified fatty acid oil phase, and a part is exposed to the water phase; then the natural polysaccharide chitosan is electrostatically adsorbed with starch, so that the surface of the starch particles exposed in the water phase is combined with chitosan nanoparticles to achieve the purpose of asymmetric modification. The present invention prepares Janus particles combined with two polysaccharides for the first time, which can combine the characteristics and advantages of two biomacromolecules, and further expand the application of Janus particles in the field of food or medicine. The raw materials used in the present invention are green, environmentally friendly and natural polysaccharide materials with good biocompatibility, and have broad application prospects.

The invention belongs to the field of pesticide preparation, and particularly relates to a porous chitosan-azoxystrobin nano slow-release agent as well as a preparation method and application thereof. Comprising the following steps: S1, weighing paraffin, Span-80 and Tween-80, stirring and mixing, and heating and stirring to obtain an oil phase; s2, weighing azoxystrobin raw medicine, water, 1% acetic acid and chitosan, and stirring and mixing to obtain a water phase; and S3, slowly dripping the water phase into the oil phase, and mixing and emulsifying to obtain the emulsion. And S4, slowly adding glutaraldehyde l and anhydrous acetic acid into the emulsion, and continuously heating and stirring at 60 DEG C for 1 hour to obtain the porous chitosan-azoxystrobin nano slow-release agent emulsion. And S5, centrifuging the emulsion, and alternately washing with petroleum ether, dichloromethane or trichloromethane to obtain the porous chitosan-azoxystrobin nano slow-release agent. According to the invention, porous chitosan nanoparticles with uniform morphology and particle size are prepared at room temperature by adopting an interfacial polymerization method, and when the porous chitosan nanoparticles are used in the field, the lasting period of the pesticide can be prolonged, the pesticide application frequency is reduced, and the pesticide application amount is reduced.

The present invention belongs to the technical field of contact lens preparation, and discloses an antibacterial coating for contact lenses and a contact lens with an antibacterial coating and a preparation method thereof. The antibacterial coating of the present invention comprises the following components by mass: 2 to 4 parts of hydroxypropyltrimethylammonium chloride chitosan nanoparticles, 5 to 8 parts of chlorhexidine acetate, 2 to 4 parts of asiaticoside, 10 to 15 parts of hyaluronic acid, 2 to 6 parts of γ-polyglutamic acid, 10 to 15 parts of polyvinyl alcohol and 1 to 5 parts of a buffer. The antibacterial coating for contact lenses of the present invention has excellent antibacterial properties, and can significantly reduce the number of fungi on the surface of the contact lens coated with the antibacterial coating; the angular contact angle of the antibacterial coating for contact lenses can be as low as 50°, so that the contact lenses coated with the antibacterial coating of the present invention have excellent moisturizing properties, thereby improving the comfort of the contact lenses when worn.

The invention relates to a preparation technology of an active bio-based food preservative film with high barrier property and high antibacterial rate, in particular to the field of food packaging and food preservation. The method comprises the following steps: by taking chitosan as a matrix, firstly, loading gallic acid by using nanoparticles prepared from the chitosan and sodium tripolyphosphate; and then, adding the chitosan nanoparticles loaded with gallic acid into a chitosan polymer network to prepare a chitosan-nanoparticle composite film, and preparing the film with high flexibility, high barrier property and high antibacterial activity. The aquatic product preservative has excellent mechanical property, barrier property, biological activity and biodegradability, can effectively prolong the shelf life of aquatic products, and has wide application prospects in the fields of food industry, preservation and the like.

The invention belongs to the technical field of medicines, and particularly relates to a composition for relieving and repairing testis injury caused by heat stress and application of the composition. The composition for alleviating and repairing testis injury caused by heat stress comprises the following raw materials: a lycopene inclusion compound, ginsenoside Re, modified chitosan nanoparticles and a solvent, the use amount of the modified chitosan nanoparticles is 10 to 15 weight percent of the ginsenoside Re. A combined formula with lycopene and ginsenoside Re as cores is innovatively designed, the two main effective components in the formula are natural plant or traditional Chinese medicine extracts, conflicts between traditional Chinese medicine properties are effectively avoided, and the lycopene and ginsenoside Re combined formula has good biological safety and is suitable for being used as a traditional Chinese medicine preparation. The composition can be used for remarkably repairing testis injury caused by heat stress and accelerating recovery after heat stress injury.

This invention belongs to the field of curcumin-loaded carrier technology, and discloses a modified chitosan carrier, its preparation and application, and modified chitosan nanoparticles loaded with curcumin. Preparation method: Under magnetic stirring in an ice-water bath, a modifier is added dropwise to a chitosan solution, and the reaction is carried out at room temperature with stirring to obtain a product liquid. This product liquid is mixed with glacial acetic acid, dialyzed, and filtered to obtain a clear and transparent solution. When the modifier is an aqueous solution of trimethylammonium chloride, the clear and transparent solution is concentrated by rotary evaporation and freeze-dried to obtain the modified chitosan carrier. When the modifier is an aqueous solution of acrylamide, the clear and transparent solution is concentrated by rotary evaporation and hydrolyzed to obtain a hydrolysate, which is mixed with glacial acetic acid, dialyzed, and freeze-dried to obtain the modified chitosan carrier. This invention significantly improves the encapsulation efficiency, drug loading, and delivery stability of curcumin in the modified chitosan nanoparticle carrier prepared by this invention.

The invention relates to the technical field of biopesticides, and provides a slow-release insecticide for landscaping and a preparation method thereof.The slow-release insecticide is prepared from, by weight, 5%-20% of clothianidin, 1%-5% of cyromazine, 1.5%-2.5% of metalaxyl hymexazol, 0.8%-1.2% of humic acid, 5%-8% of chitosan, 3%-4% of polylactic acid, 1.5%-2.5% of nanometer titania, 0.5%-0.8% of plant source auxiliaries and the balance water; compared with existing insecticides, a'stepped release 'system (clothianidin rapidly controls insects in the early stage and cyromazine is long-acting and stable in the later stage) is formed through a nano slow release technology (chitosan nanoparticles and PLA microspheres), synergistic prevention and control of overground and underground insects are effectively achieved, the instantaneous concentration of the insecticide in the environment is reduced, and the resistance selection pressure is reduced.

The invention provides a preparation method and equipment of a composition helpful for controlling carbon water absorption. According to the preparation method, the phyllanthus emblica, the sugarcane polyphenol, the white kidney bean extract, the mulberry leaf extract and the L-arabinose are respectively embedded by adopting chitosan nanoparticles, so that the stability of active ingredients is improved, and fixed-point release of intestinal tracts is realized. And uniformly mixing the obtained embedded particles, protein powder, dietary fibers and other raw materials through a three-dimensional mixing machine, and carrying out composite molding on the obtained mixture and a sugar-oil mixture to prepare the high-protein meal replacement bar which plays a role in synergistically controlling sugar and promoting lipid metabolism. According to the preparation equipment, an auxiliary mixing assembly is additionally arranged in a mixing barrel and comprises a guide bearing rod distributed along a barrel shaft and an auxiliary mixing part capable of sliding along the guide bearing rod, the mixing barrel moves to drive the auxiliary mixing part, and meanwhile reciprocating sliding is achieved through the gravity effect. According to the structure, the powder overturning and dispersing effects are enhanced, agglomeration is effectively broken, the mixing uniformity and stability are improved, and the mixing efficiency is remarkably improved under the condition of low energy consumption.

This application relates to an anti-icing coating, its preparation method, and its application, belonging to the field of protective coating technology. The anti-icing coating comprises a superhydrophobic self-cleaning coating and a hydrophobic and oleophobic layer stacked together. The superhydrophobic self-cleaning coating includes first SiO2 nanoparticles and fluorosilane. The hydrophobic and oleophobic layer includes second SiO2 nanoparticles, nanocellulose, chitosan nanoparticles, and a binder. The use of natural polymer materials such as nanocellulose and chitosan nanoparticles in the hydrophobic and oleophobic layer results in lower cost and better environmental friendliness. The superhydrophobic self-cleaning coating achieves superhydrophobic properties through the synergistic effect of a nano-dispersion system and a "papillary" micro-nano rough structure. The hydrophobic and oleophobic layer further enhances the coating's hydrophobic and oleophobic properties and surface lubricity, significantly reducing the adhesion of ice to the coating surface.

This invention is related to the fields of medical biotechnology, nanobiotechnology, and nanomedicine. It involves a nanomedicine product that can release HOC1 (hypochlorous acid) at the tumor site in a controlled manner to treat lung cancer. HOC1 is a potent antimicrobial and antitumor agent that can target tumor tissues while minimizing damage to healthy tissues. The produced nanoparticles have a two-layer structure: the inner layer contains alginate, PFP (perfluoropentane), and Ca(OCl)2 to release HOC1, and the outer layer has a chitosan coating with folate and salicylic acid for targeting and proton release. The production process involves nanoemulsion and coating with the ionic gelation technique. When the nanoparticles are delivered to the lungs and targeted to cancer cells with folate, ultrasound radiation triggers the release of HOC1 to induce apoptosis and destroy cancer cells effectively, reducing side effects and improving treatment outcomes for lung cancer patients.

The invention provides a drug delivery system, the drug delivery system is formed by cross-linking and curing hyaluronic acid conductive hydrogel and chitosan nanoparticles modified by chlorotoxin, the hyaluronic acid conductive hydrogel is formed by cross-linking and curing hyaluronic acid-methacrylic anhydride and reduced graphene oxide, and the chitosan nanoparticles modified by chlorotoxin are formed by cross-linking and curing hyaluronic acid-methacrylic anhydride and reduced graphene oxide. The chlorotoxin-modified chitosan nanoparticles are formed by loading a therapeutic drug on chitosan nanoparticles, coupling chlorotoxin by adopting an EDC / NHS activation method, and modifying by PEG (Polyethylene Glycol). The invention also provides a preparation method of the drug delivery system. The invention also provides application of the drug delivery system in preparation of drugs for treating tumors. According to the invention, two stages of carriers are used for synergistically and doubly loading therapeutic drugs, so that the conductive function, biocompatibility, drug loading stability and release controllability are considered, and the MGMT high-expression mediated tumor drug resistance is effectively reversed.

The invention relates to an anti-inflammatory plasma matrix as well as a preparation method and application thereof. The anti-inflammatory plasma matrix comprises a plasma matrix and aspirin chitosan nanoparticles, the preparation method of the anti-inflammatory plasma matrix comprises the step of pressing a mixture of the plasma matrix and the aspirin chitosan nanoparticles to obtain the anti-inflammatory plasma matrix. Leukocyte contained in the plasma matrix in the anti-inflammatory plasma matrix can play an antibacterial role, aspirin can be slowly released by the aspirin chitosan nano-particles, the function of regulating and controlling bone regenerative cycle in an inflammatory state is achieved, and a chitosan shell also has certain antibacterial and anti-inflammatory effects. The prepared anti-inflammatory plasma matrix can be applied to preparation of drugs for treating periodontitis and peri-implantitis.

The present invention relates to nucleic acid fragment-encapsulated polymer nanoparticles and a method of preparing the same. The polymer may be a mucoadhesive polymer and specifically chitosan, and the nucleic acid fragment may be polydeoxyribonucleotide (PDRN). The nucleic acid fragment-encapsulated polymer nanoparticles may be used as an eye drop. Since the nucleic acid fragment-encapsulated polymer nanoparticles is capable of adhering to the mucus in the eyes, nucleic acid fragments inside the nanoparticles can be slowly released into the ocular mucous membrane. In addition, since nucleic acid fragment-encapsulated polymer nanoparticles can exhibit high efficiency in the eyes even with a small dose of drug, patient convenience can be increased or costs can be reduced.

The invention belongs to the technical field of functional biopolymer materials, and relates to alkyl alkenyl succinic anhydride modified chitosan nanoparticles as well as a preparation method and application thereof. The nano-particles are formed by self-assembling after chitosan is subjected to hydrophobic modification through alkyl alkenyl succinic anhydride and the pH of a system is adjusted under the condition from acidity to neutrality or alkalescence. The degree of substitution of the nanoparticles is 1-40%. The invention also provides a preparation method and application of the nanoparticles. The nanoparticles are suitable for various alkyl alkenyl succinic anhydrides with the carbon chain length of C6-C16, and a new technical scheme is provided for constructing an oil-water dispersion system stabilized by bio-based particles.

The present invention, based on a non-bubble-based sonoporation technique, provides an ultrasound-assisted delivery platform for drug-loaded chitosan nanoparticles. This platform comprises: the administration of drug-loaded chitosan nanoparticles and the application of ultrasound stimulation. The drug-loaded chitosan nanoparticles are delivered to a target site, where ultrasound waves are directly applied to induce cellular membrane deformation. The ultrasound mechanical force temporarily alters membrane permeability, thereby facilitating the intracellular delivery of the drug-loaded chitosan nanoparticles.

The invention relates to the field of antifouling coatings, and particularly discloses a green environment-friendly water-based industrial antifouling coating and a preparation method thereof. The antifouling paint comprises 40-55% of bio-based FEVE resin, 8-15% of a bio-based functional additive, 5-8% of nano titanium dioxide, 8-12% of wollastonite powder, 5-7% of calcium carbonate, 3.0-4.5% of other auxiliaries and the balance of water, wherein the bio-based functional additive comprises palm oil fatty glyceride, chitosan nanoparticles and a bamboo fiber / nano cellulose compound. According to the invention, the vegetable oil modified FEVE resin is used as a core raw material to replace a traditional petroleum-based raw material, and is matched with a relatively small addition amount of the bio-based functional additive and fillers such as nano titanium dioxide, wollastonite powder and calcium carbonate, so that the antifouling paint with environmental friendliness, hydrophobicity, stain resistance and weather resistance is prepared, the purpose of long-acting antifouling is achieved, and the environment-friendly, hydrophobic and weather-resistant effects are achieved. Moreover, the production cost is lower, and the application prospect is very wide.

The invention discloses an environment-friendly marine antifouling nano coating and a preparation method thereof, and the nano coating comprises 10-20 parts of nano particles, 100 parts of aqueous film-forming resin, 10-20 parts of a low surface energy auxiliary agent, and 3-5 parts of a biological antifouling agent. The nano particles comprise modified inorganic nano particles grafted by 3-aminopropyltriethoxysilane and chitosan nano particles crosslinked by sodium tripolyphosphate, and the mass ratio of the modified inorganic nano particles to the chitosan nano particles is (2: 1)-(3: 1). According to the environment-friendly marine antifouling nano coating and the preparation method thereof, the characteristics of small size effect, high specific surface area and the like of a nano material are utilized, and the advantages of the nano material in the coating are fully embodied by controlling the particle size and the preparation process, such as improving the adhesive force, enhancing the strong acid and strong alkali resistance, blocking the invasion of chloride ions and the like; therefore, the overall quality of the coating is improved.

The application belongs to the technical field of medicine, and particularly relates to a composition for alleviating and repairing testicular damage caused by heat stress and application thereof. The composition for alleviating and repairing testicular damage caused by heat stress comprises the following raw materials: lycopene inclusion compound, ginsenoside Re, modified chitosan nanoparticles and a solvent. The amount of the modified chitosan nanoparticles is 10-15 wt% of the ginsenoside Re. The application innovatively designs a combination formula with lycopene and ginsenoside Re as the core. Both of the two main effective components in the formula are natural plant or traditional Chinese medicine extract, which effectively avoids the conflict between the medicinal properties of traditional Chinese medicines and has good biological safety. The composition can significantly repair the testicular damage caused by heat stress and accelerate the recovery after heat stress damage.

The present invention provides compositions and methods for detecting tumor tissues in a subject. Embodiments of the present invention provide biocompatible chitosan nanoparticles suitable for dual modality optical imaging. Embodiments of the present invention provide methods for fabricating chitosan nanoparticles. Embodiments of the present invention provide methods for detecting tumor tissues in a subject using compositions provided by the present invention.

The invention belongs to the technical field of biological medicines, and particularly discloses a nano drug-loaded catheter coating material based on TUBA1C gene expression regulation and a preparation method thereof. The coating material is composed of siRNA (small interfering ribonucleic acid) loaded with TUBA1C and PLGA (poly (lactic-co-glycolic acid)) / chitosan nanoparticles loaded with antibacterial peptide GL13K. The sequences of the siRNA are as shown in SEQ ID NO: 1 and SEQ ID NO: 2. Nanoparticles are prepared through a multiple emulsion-solvent evaporation method, and the surface of the catheter is coated with the nanoparticles. The coating provided by the invention can synergistically exert gene silencing and antibacterial effects, and siRNA silences a TUBA1C gene in urethral mucosa cells in a targeted manner and inhibits inflammatory response and fibrosis; the antibacterial peptide GL13K can kill pathogenic bacteria and prevent the formation of biological membranes. The coating material has antibacterial, anti-inflammatory and anti-biofilm functions, can effectively prevent and treat catheter-related urinary tract infection, and reduces the risk of long-term complications such as urethrostenosis.

The invention discloses a soluble microneedle array transdermal drug delivery freckle-removing mask and a preparation method of the soluble microneedle array transdermal drug delivery freckle-removing mask. The mask comprises a backing layer and a soluble micro-needle array arranged on the backing layer, the micro-needle array is composed of a needle tip and a base, and the needle tip comprises a freckle-removing active component, a nano-carrier material, a micro-needle matrix material and a stabilizer. The freckle-removing active components comprise tranexamic acid, sodium ascorbyl phosphate, Maojian green tea extract, licoflavone and nicotinamide; the nano carrier material is selected from hydroxyl cyclodextrin, chitosan nanoparticles and alginic acid-chitosan nanoparticles; the microneedle matrix material comprises hyaluronic acid, polyvinylpyrrolidone and polyvinyl alcohol. Through combination of a nano-carrier technology and a micro-needle system, the drug loading capacity, stability and transdermal efficiency of freckle-removing active ingredients are remarkably improved, and the freckle-removing micro-needle has the advantages of being good in freckle-removing effect, painless, noninvasive, convenient to use and the like, and is suitable for prevention and treatment of chloasma, freckles, hyperpigmentation after inflammation and other pigmented skin diseases.

The invention relates to the technical field of corn, and particularly discloses a biological fermentation cornflake and a preparation method thereof. The preparation method of the biological fermentation cornflake comprises the following steps: dissolving glucose, peptone and microbial growth factor mixed salt in water, adding lactobacillus plantarum freeze-dried powder, stirring, culturing, centrifuging, collecting supernate, adding a chitosan nanoparticle solution, stirring, adding algal polysaccharide-protein gel and an activated lactobacillus liquid, and stirring to obtain a pretreated bacterial liquid; and dispersing corn in water, standing, fishing out, steaming with a steamer, pressing with a tablet press, cooling, adding the pretreated bacterial liquid, stirring, fermenting, and drying to obtain the biologically fermented corn flakes. The biological fermentation cornflake is good in crispness, high in nutrient component retention rate and simple in preparation method.

The invention discloses a preparation method and application of folic acid modified beta-acid loaded chitosan nanoparticles. The preparation method comprises the following steps: (1) dissolving FA, NHS and EDC in DMSO according to a molar ratio of 1: 1.5: 1.5; (2) adding chitosan into a 1% acetic acid solution, and stirring for dissolving; (3) adding the activated folic acid active ester in the step (1) into the chitosan aqueous solution in the step (2), and mixing and stirring to obtain a mixed solution 1; (4) adding a beta-acid ethanol solution into the mixed solution 1 prepared in the step (3), and stirring to obtain a mixed solution 2; and (5) mixing and stirring a sodium tripolyphosphate solution and the mixed solution 2 prepared in the step (4), and centrifuging to obtain the folic acid modified beta-acid loaded chitosan nanoparticles. The nanoparticles prepared in the invention not only have a certain bacteriostatic effect on staphylococcus aureus, but also can be successfully ingested by HCT116 cells, and in-vivo safety experiments of mice find that the drug-loaded nanoparticles are safe.