372 results about "Chitosan nanoparticles" patented technology

The present invention relates to a water soluble chitosan nanoparticle (WSC-NP) for delivering an anticancer agent and a preparing method thereof, more precisely, a water soluble chitosan nanoparticle for delivering an anticancer agent which has function of targeting on a wanted area by introducing a functional group in the location of highly reactive amine group and becomes an excellent gene carrier with the use of water soluble chitosan since the water soluble chitosan itself can combined with DNA having a negative electric charge(−) owing to the very strong positive electric charge(+) of its amine group, and a preparing method thereof. Therefore, a water-soluble chitosan nanoparticle for delivering an anticancer agent of the present invention can effectively envelope paclitaxel by introducing hydrophilic and hydrophobic groups in the position of highly reactive amine group of the water-soluble chitosan. A water soluble chitosan nanopaclitaxel prepared as the above has an excellent re-dispersion force, after freeze-drying, in distilled water and has an outstanding anticancer effect with its accumulation in tumor cells greater than that of the other anticancer agent carriers.

The present invention discloses a chitosan nanoparticle suspension preparation method and application thereof. The chitosan nanoparticle suspension preparation method comprises the following steps of: (1) dissolving a chitosan into a glacial acetic acid aqueous solution to prepare a chitosan aqueous acetic acid; (2) under stirring, adding a tripolyphosphate sodium aqueous solution dropwise to obtain a coarse chitosan nano suspension; and (3) after filtering the coarse chitosan nano suspension, processing homogeneously the filtered suspension with high pressure to obtain a nano chitosan suspension with a uniform particle size distribution. The invention has the advantages of simple operation, good scale-up preparation reproducibility, and low production cost, and is applicable to industrial production. The prepared nanoparticles are of a uniform morphology, small in particle diameter and applicable to preparing products with functions of antibiosis, hemostasis and promoting tissue repair.

The invention discloses a kernel-shell structural nanofiber membrane, a preparation method thereof and an application; the preparation method includes steps of (1), preparation of spinning; dissolving sodium alginate, polyoxyethylene and poloxamer F127 in distilled water, stirring evenly and acquiring shell layer spinning solution; mixing the chitosan nanoparticle solution loaded with active matters with polyving akohol solution evenly, and acquiring the kernel layer spinning solution; (2), coaxial electrostatic spinning: respectively injecting the shell layer spinning fluid and kernel layer spinning fluid in a static spinning device containing a coaxial needle, performing the coaxial electrostatic spinning under room temperature, performing solvent evaporation in the spinning process, and acquiring the kernel-shell structural nanofiber membrane loaded with the nanoparticle. The preparation method is simple in operation and gentle in reaction condition and can well control the slow release of the active matter; moreover, the prepared colon targeting controlled release system has obvious colon targeting property, thereby improving the utilization degree of the active matter. The colon targeting controlled release system can be applied to functional food domain.

The invention relates to a intraocular lens. At present the Poly-Methyl Methacrylate (PMMA) intraocular lens conventional for clinical treatment of cataract always causes inflammatory treaction after implantation. Posterior capsule opacification is also a major compalication after cataract surgery. The invention selects fluorouracil to solve the above problems. Based on weak penetrating force of chitosan nanoparticles in eyes and the relation between the phagocytosis amount of conjunctival epithelial cells to nanoparticles and the particle size of nanoparticles, the fluorouracil nanoparticles preparation is prepared using chitosan-poly(acrylic acid) as carrier, composite coated on the surface of PMMA intraocular lens. The invantion also provides a preparation method thereof. The said intraocular lens not only increases the biocompatibility of the intraocular lens, but also inhibites posterior capsule opacification pafter cataract surgery. The said intraocular lens can also prevent anterior membrane after implantation of intraocular lens and after-cataract.

The invention discloses a novel method for preparation of a chitosan nano carrier and functionalization thereof, which relates to the chitosan nano carrier. The invention provides a novel method for preparation of the chitosan nano carrier and functionalization thereof. The chitosan is dissolved in acetic acid solution, meanwhile, pH is adjusted to 4.5-5.5 with NaOH solution, STPP (sodium tripolyphosphate) solution is added to the chitosan solution to obtain nanogels, then, crosslinking glutaraldehyde is provided for the chitosan, centrifugation is conducted after ending of reaction, reduction reaction is conducted with excess sodium borohydride, then centrifugation is conducted again, then, the obtained compound is dispersed in hydrochloric acid solution to remove the unreacted sodium borohydride, then dialysis is conducted, in order to wash STPP to obtain nanoparticles; the folic acid is weighed to be dissolved in the phosphate buffer solution, then the solution is added to chitosan nanoparticles water solution, after adding EDCI to the solution, folic acid modified nanoparticles are obtained through reaction; PEG succinimidyl propionate is weighed to be dissolved in the phosphate buffer solution, then is added to the chitosan nanoparticles water solution, after reaction, PEG modified nanoparticles are obtained.

The invention relates to a preparation method and application of tea polyphenol chitosan nanoparticles, in particular to novel tea polyphenol nanoparticles. The nanoparticles are prepared from the following components in parts by weight: 3 to 6 parts of chitosan, 1 to 2 parts of porous silicon dioxide and 1 to 4 parts of tea polyphenol. The porous silicon dioxide is added into the tea polyphenol nanoparticles, so that the tea polyphenol content of the nanoparticles can be remarkably increased. The tea polyphenol chitosan nanoparticles are prepared by a sodium tripolyphosphate (TPP) ionic gel method. The nanoparticles can be widely applied to various fields of biomedicine and health care products, and can be used for lowering blood cholesterol, treating obesity, resisting oxidation, clearing free radicals and preventing and treating tumors.

The invention belongs to the field of drug nanopreparation, especially relates to a chitosan nanoparticle preparation of ceftiofur sodium, and a preparation method thereof. The chitosan nanoparticle preparation is characterized in that: the preparation comprises 3-7 mg / mL of chitosan, 1.13-2.63 mg / mL of sodium tripolyphosphate and 2-5 mg / mL of ceftiofur sodium. The preparation method adopts an ionic crosslinking method and comprises the following steps: dissolving the chitosan, dissolving the ceftiofur sodium in the sodium tripolyphosphate solution, adding the sodium tripolyphosphate solution containing the ceftiofur sodium to the chitosan solution under magnetic stirring, and the like. According to the present invention, the ceftiofur sodium chitosan nanoparticles have target distribution and slow release property, such that the action time of the drug can be prolonged, the efficacy can be increased, the toxic and side-effect of the drug can be reduced, the antibacterial stability of the drug can be improved; the preparation method has characteristics of simpleness, reliability and controllable quality, and is suitable for the industrial production.

The invention provides a magnetic chitosan nanoparticle adsorbent. The structural formula of the magnetic chitosan nanoparticle adsorbent is as shown in the specification. The invention also provides a preparation method of the magnetic chitosan nanoparticle adsorbent. The magnetic chitosan nanoparticle adsorbent provided by the invention is even in grain size, good in dispersibility, high in specific saturation magnetization and abundant in active group quantity has a good removal effect on natural organic matters. The preparation method is simple and easy to control the preparation process. The prepared magnetic chitosan nanoparticle adsorbent is stable in quality.

The present invention provides for curcumin nanoparticles and curcumin bound to chitosan nanoparticles and methods of producing the same. Bioavailability of curcumin in these formulations was shown to improve by more than 10 fold.

Belonging to the field of antibacterial preservatives and food fresh-keeping packaging materials, the invention in particular relates to a nisin / chitosan nano particle antibacterial film, a preparation method and application. Nisin crosslinks with gamma-polyglutamic acid, then chitosan is added to undergo crosslinking with gamma-polyglutamic acid so as to prepare nisin loaded gamma-polyglutamic acid / chitosan nano particles, thus improving the defect of bioactivity decrease of nisin during use and enhancing the utilization rate, then the nisin loaded chitosan nano particles are prepared into an antibacterial film by means of electrospinning so as to reduce the aggregation and drop phenomena of chitosan nano particles during direct sprinkling to food surfaces and avoid bioactivity decrease of encapsulated substances, thereby reaching the purposes of long-acting antibacterial properties and efficient utilization. According to the invention, the operation is simple, by applying the antibacterial film to fresh-keeping of cheese products, growth of pathogenic microorganisms can be effectively inhibited, and the shelf life of products can be prolonged.

The invention discloses abscisic acid-embedded chitosan nanoparticles and a preparation method thereof. The abscisic acid-embedded chitosan nanoparticles are nanoparticles formed by wrapping aqueous solution of abscisic acid by an embedding material which is formed by dilute solution of acetic acid of chitosan and a crosslinking agent. The preparation method comprises the following steps of: adding the chitosan into the dilute solution of acetic acid to be dissolved, and then adding solution of sodium hydroxide to adjust the pH value to obtain dilute solution of acetic acid of the chitosan; preparing abscisic acid into aqueous solution; preparing the crosslinking agent into aqueous solution; adding the solution of dilute acetic acid of the chitosan into the aqueous solution of abscisic acid, and then adding the aqueous solution of crosslinking agent drop by drop; and fully mixing uniformly to obtain the abscisic acid-embedded chitosan nanoparticles. The method is simple and easy, and has high embedding rate; the obtained embedding nanoparticles have no adhesion, good glomeration and round surface; after the embedding, the abscisic acid can be protected from external interference, and the stability of the abscisic acid can be improved; the abscisic acid is steadily and slowly released, which prolongs action time and strengthens induced resistance effect; and the chitosan serving as a wall material has disease-resistant and induced resistance effects.

The invention relates to chitosan (CS) vehicles with chitosan nanoparticles, as well as methods for making such chitosan vehicles and for using them to carry a DNA or proteins by forming CS-DNA or CS-protein complexes. The present invention also relates to CS-DNA or CS-protein complexes being useful for transdermal delivery of DNA or protein with a low-pressure gene gun. In another aspect, the present invention also relates to CS-DNA or CS-protein complexes being useful for transcutaneous delivery of a DNA or protein with a skin patch. Further aspects of the present invention relate to methods for making CS-DNA or CS-protein complexes and for using them for diagnostic, therapeutic and biological industrial applications.

A delivery system employing nanoparticles of thiolated chitosan which complex with nucleic acids or other drugs. The system utilizes an approximately 33 kDa thiol-modified chitosan derivative resulting in highly effective gene delivery. Thiolation of chitosan resulted in reduced density of positive charges and DNA binding capacity. However, thiolated chitosan carrying a plasmid DNA expressing a green fluorescent protein (GFP) showed significantly higher GFP expression in various cell lines and in vivo in mice. Sustained delivery of plasmid DNA from thiolated chitosan was achieved by crosslinking thiolated chitosan / plasmid DNA nanocomplexes through inter- as well as intramolecular disulfide bonds under the physiological conditions. Thiolated chitosan nanoparticles have a great potential for gene therapy and tissue engineering.

The invention discloses a CTC (circulating tumor cell) capturing and purifying substrate based on chitosan nanoparticles as well as a preparation method and an application of the CTC capturing and purifying substrate. Chitosan with good cytocompatibility is selected for constructing a three-dimensional nanostructure 'soft' substrate, anti-adhesion molecules such as PEG (polyethylene glycol) molecules are introduced to reduce the nonspecific adhesion of cells on an interface, affine CTC capturing molecules such as EpCAM (epithelial cell adhesion molecule) aptamers are used for high-specific capturing of CTCs, and the purity of target cells is further improved in an in-situ culturing manner of the captured cells on the basis of proliferation attribute difference of the CTCs and blood cells. The CTC capturing and purifying substrate has good cytocompatibility and can keep the activity of the captured cells, a preparation process is simple and convenient, the cost is low, in-situ culturing and purification of the captured cells can be realized, and large-scale implementation is easy to realize.

The invention relates to an enteric material coated chitosan nanoparticle which covers insulin and cell-penetrating peptide Tat and a preparation method of the chitosan nanoparticle. The chitosan nanoparticle is prepared from an enteric material, namely Eudragit S100, insulin, cell-penetrating peptide and chitosan. With the oral administration of the prepared enteric coated insulin chitosan nanoparticle, the amount of loaded contents released from the chitosan nanoparticle in the stomach and the upper part of the small intestine can be greatly reduced due to the protective effect of the enteric material, namely Eudragit S100. As the chitosan nanoparticle arrives at the lower end of the digestive tract, the enteric coating material on the surface of the chitosan nanoparticle begins to dissolve and the chitosan nanoparticle becomes exposed with the increase of pH value; the chitosan nanoparticle is damaged under a weakly alkaline condition, so that the cell-penetrating peptide and the insulin therein are released. The cell-penetrating peptide is capable of prompting the absorption of colonic epithelial cells to the insulin so as to improve the bioavailability of the insulin through oral administration.

The invention discloses a method for preparing magnetic chitosan nano particles and processing heavy metal wastewater, belonging to the technical field of material preparation. The method disclosed by the invention comprises the following steps: firstly, weighing ferrous salt and ferric salt as raw materials for preparing a magnetic material, wherein the raw materials are dispersed in a chitosan acetic acid solution after being dissolved; and dropping the mixed liquor into alkali liquor through an injector to obtain the magnetic chitosan particles, wherein the prepared magnetic chitosan particles are provided with a chitosan-nano ferroferric oxide core shell structure and a nano biological absorption material with excellent absorption performance. The method disclosed by the invention is simple and easy to operate. The nano biological absorption material prepared by the method disclosed by the invention is used for processing the heavy metal wastewater.

The invention provides an efficient solidifying and stabilizing repair agent aiming at heavy metal contaminated soil. The efficient solidifying and stabilizing repair agent comprises the following raw materials: gypsum, magnesium oxide, kieselguhr, carboxymethylcellulose-melamine formaldehyde resin and hydroxypropyl-modified SiO2 / chitosan nanoparticles. According to the agent provided by the invention, two organic matters are added, so that the additive amount of the inorganic matters can be greatly reduced, and the property of the soil is not greatly destroyed; meanwhile, the two organic matters are not easily decomposed in the soil, and the toxic level of the heavy metals in the environment still can be well reduced on the basis of reducing the damage to the property of the soil.

The invention belongs to the technical field of nanometer material preparation, and relates to a preparation method of chitosan nanoparticles, in particular to a preparation method and an application of the chitosan nanoparticles with pore structures. According to the preparation method and the application of the chitosan nanoparticles with the pore structures, first, chitosan is dissolved into acetic acid solution, and chitosan solution is obtained; second, span-80 is dissolved into organic solvent to obtain oil phase, third, the chitosan solution and the oil phase is cut and emulsified in a high speed, and opposite phase miniemulsion is obtained; the opposite phase miniemulsion is stirred and cross linker solution is dropped into the opposite phase miniemulsion to react; and then a system is decompressed, dehydrated, centrifugated, washed and vacuum dryed, so that the chitosan nanoparticles with the pore structures are obtained. According to the preparation method and the application of the chitosan nanoparticles with the pore structures, the grain diameters of the chitosan nanoparticles with the pore structures are ranged from 100 nm - 500 nm, and the chitisan nanoparticles with the pore structures are good carriers of medicines, and can be used for medicine absorption. Meanwhile, in preparation process, water phase and the oil phase inside the opposite phase miniemulsion are similar, so that the production efficiency of nanoparticles is high.

A method of making ultra-small chitosan nanoparticles having a size range of approximately 10-20 nm, includes preparing a first microemulsion containing effective amounts of cyclohexane, n-hexanol, chitosan polymer and a nonionic surfactant. A second microemulsion is prepared containing effective amounts of cyclohexane, n-hexanol, tartaric acid, EDC, n-hydroxysuccinimide, and a nonionic surfactant. The method continues by reacting the first and second microemulsions for a time sufficient to form the ultra-small chitosan nanoparticles and recovering the nanoparticles from the reacted microemulsion. The chitosan polymer may be crosslinked and may also be tagged with a fluorescent compound, a radio-opaque compound, a paramagnetic ion, a ligand specific for a predetermined biologic target, a drug, and combinations thereof.

The invention belongs to the field of antibacterial preservatives and food fresh-keeping packaging materials and particularly relates to a method for preparing a load D-amino acid / cinnamon essential oil nanofiber antibacterial film and application. By wrapping D-amino acid and cinnamon essential oil with chitosan nano particles, volatilization of the essential oil is reduced, and the essential oil utilization rate is improved. The D-amino acid / cinnamon essential oil / chitosan nano particles are added into an electrostatic spinning solution to prepare the nanofiber antibacterial film, so as to reduce gathering and descending phenomena arising when the chitosan nano particles are directly sprayed on the surface of a food, and then the antibacterial film undergoes auxiliary processing through a cold plasma and then is used for restraining formation of a staphylococcus aureus biological film on a bean product. The method is simple to operate, the biological film is applied to refreshment of soybean products, so growth of pathogenic microorganisms can be effectively restrained, the guarantee period of the products is prolonged, and the method has high market value.

The invention belongs to the technical field of medicines, and relates to an antigen covalently bound chitosan nanoparticle-based nasal immune carrier and a preparation method thereof. The carrier is composed of a nanoparticle and an antigen, the antigen is covalently bound to the surface of the nanoparticle, and the nanoparticle is prepared by adopting a raw material chitosan and its derivatives; and the antigen is selected from from infectious diseases or tumors related proteins or polypeptides. The carrier can significantly improve the neck lymph node targeting of people nasally dosed with the antigen proteins or polypeptides, enhances the mucosal and systemic immune response, has the advantages of simple preparation, stable process, high antigen utilization rate, difficult antigen shedding, benefiting of the improvement of the cervical lymph node transportation of the antigens, good nasal immune effect and no nasal mucosa toxicity, and is suitable for preventing respiratory infectious diseases.

The invention relates to a preparation method of water-soluble chitosan nano-particles which can be used as drug carriers. The chitosan nano-particles are prepared by adopting an emulsification cross-linking process, which comprises the following steps of: adding a chitosan aqueous solution into liquid paraffin added with an emulsifier at first, preparing W / O type emulsion, adding a genipin solution to crosslink, then, adjusting a pH value by using a NaHCO3 solution, rinsing by using petroleum ether after reacting, and drying in vacuum to obtain the chitosan nano-particles. The nano-particles prepared by using the method disclosed by the invention are good in sphericity and low in toxicity and can be used as the drug carriers.

The invention discloses a litopenaeus vannamei preservative which is characterized by being prepared from the following raw materials in parts by weight: 15 parts of nano chitosan, 5-7 parts of ascorbic acid (Vc), and 1 part of phytic acid, wherein the nano chitosan is prepared by adopting a following method: adding chitosan in 1% acetic acid solution to prepare 0.5mg / mL chitosan solution, regulating pH value to 3.4-4.8 by using 1 mol / L NaOH solution; and dropwise adding 1mg / mL sodium tripolyphosphate solution in the chitosan solution so as to obtain a mixed solution, ensuring that the mass ratio of the sodium tripolyphosphate to the chitosan reaches 1:2, magnetically stirring the mixed solution at a room temperature for 60min continuously, and crosslinking to chitosan nano particles through ionic reaction. The nano chitosan is compounded with the phytic acid and the ascorbic acid, so that a synergistic effect can be developed, and a relatively good refreshing effect is achieved in comparison with that of a manner of singly using the nano chitosan, the phytic acid and the ascorbic acid. The compound preservative disclosed by the invention is excellent in antibacterial effect, and is capable of enabling meat of litopenaeus vannamei to keep delicious and keeping the attractive appearance in a storage period.

The invention provides an active peptide embedded nanoparticle and a preparation method thereof and aims to solve the problem that albumen derived active peptides which are easily degradable in a gastrointestinal digestive system cannot be completely absorbed currently. By adoption of ionic gel, chitosan and sodium tripolyphosphate serve as nano coating materials to realize embedding of albumen derived active peptides, with four different molecular weights, obtained by enzymolysis, and consequently the albumen peptide-chitosan nanoparticle great in embedding performance is obtained. A gap in protection and promotion of biological absorption and utilization of the albumen derived active peptides is filled, a new idea is provided for deep processing in the industry of egg products, and resource integration and maximal reasonable application of the egg products are realized.

The invention belongs to the field of biological medicine, and particularly relates to a preparing method and application of a human mesenchymal stem cell source exosome bio-active preparation. The method comprises the steps of weighing and dissolving 50-400 mg of chitosan in 25-200 mL of dilute acetic acid to obtain chitosan solution, and weighing and dissolving 1-10 mg of human mesenchymal stem cell source exosome freeze-dried powder, 2-20 mg of glucosaminoglycans and 2-20 mg of hyaluronic acid in 10-100 mL of glycerin to obtain exosome solution; adding the exosome solution to the chitosan solution dropwise to obtain chitosan nanoparticle suspension of exosome; and conducting centrifugal treatment on the suspension, collecting sediment, and conducting washing and drying treatment to obtain the beautifying preparation. The problem of skin aging is solved, frequent use is avoided, adverse reaction of beautifying preparations is reduced, the effective concentration of active ingredients can be maintained for a long time, and the best treatment effect can be realized with the minimum dose.

An anti-infectious immunopotentiator for pig, ox, yak, etc is prepared through artificially synthesizing the CpG oligonucleotide sequce able to excite the reproductive activity of immune cell, preparing the chitosan nanoparticles from deacetyl chitosan, and using said chitosan nanoparticles for molecular packaging of CpG oligonucleotide. It can be used to immunize the experimental animal by intramuscular injection or oral applialtion.

The present invention belongs to the technical field of nanoparticle, and particularly relates to a mannose-modified thiolated chitosan quaternary ammonium salt nanoparticle, a preparation method and application thereof. The nanoparticle of the invention is generated through polyelectrolyte effect between mannose-modified thiolated chitosan quaternary ammonium salt with positive charges and mannose-modified thiolated chitosan quaternary ammonium salt with negative charges. Simultaneously the nanoparticle loads protein peptide medicine or nucleic acid medicine with negative charges, wherein the mannose-modified thiolated chitosan quaternary ammonium salt is obtained from amino-groups of the chitosan through quaternizing, mannose ligand and sulfhydrylation modification successively. The nanoparticle has the following advantages: no requirement for organic solvent in preparation process, and effectively improved medicine stability. The nanoparticle can actively target intestinal epithelial cells, M cells and macrophages. Intestinal absorption for the oral medicine and transfection efficiency of the nucleic acid medicine are effectively improved. The effect of the mannose-modified thiolated chitosan quaternary ammonium salt nanoparticle is much better than that of the prior-art chitosan nanoparticle.

The invention discloses a preparation method and application of chitosan particles. The chitosan particles comprise chitosan microspheres and chitosan nanoparticles. The invention provides the application of the chitosan particles in preparation of weight losing medicaments, fat lowering medicaments or foods; and by the conclusion of the researches, the chitosan microspheres have good effect in the application in the preparation of the medicaments or foods for suppressing appetite and the chitosan nanoparticles have good effect in the application in the preparation of the medicaments or foods for lowering the fat in the body. The invention provides technical support for applying the chitosan to the preparation of the medicaments or foods for losing weight better, and fulfilling the aims of effectively reducing dosage and achieving good weight losing effect.

The invention discloses a frequency-sweeping ultrasonic preparation method of rapeseed protein-chitosan nanoparticles, and relates to the technical field of composite nanoparticles of functional food. The rapeseed protein-chitosan nanoparticles are prepared from the following raw materials in parts by weight: 2-10 parts of active rapeseed protein and 1-2.5 parts of chitosan. The chitosan is added into the active rapeseed protein to change the advanced structure of the active rapeseed protein, so that the structure thereof is opened to expose active groups; meanwhile, the protein and polysaccharide form small aggregates, so that formation of the nanoparticles by the aggregates through hydrophobic interaction is promoted, and a foundation is laid for embedding a bioactive component. In the preparation process of the curcumin-embedded active rapeseed protein-chitosan composite nanoparticles, by a frequency-sweeping ultrasonic processing technology, cross-linking of the protein and the polysaccharide into the aggregates is promoted through the physical force of ultrasonic, so that the formation of the nanoparticles by the aggregates through the hydrophobic interaction is promoted and the foundation is laid for embedding the bioactive component.

The invention provides a chitosan nano drug carrier with pH and temperature response and a preparation method and application thereof. The nano drug carrier is of a core-shell structure, a core of thecore-shell structure is formed through ionic crosslinking of chitosan and sodium tripolyphosphate, and a shell is formed by accumulation of carboxyl derivative of cyclodextrin. The preparation methodcomprises the steps of activation of the carboxyl derivative of the cyclodextrin, synthesis of cyclodextrinated chitosan and preparation of cyclodextrinated chitosan nano particles. According to thechitosan nano drug carrier, hydrophobic drugs are carried to the nano particles through hydrophobic interaction, and the nano particle drug carrier has dual responsiveness to the pH and the temperature, and can be used for targeted drug delivery to improve utilization rate of the drugs.