84 results about "Nanobiotechnology" patented technology

The invention relates to a biological cell ultrasonic atomic force microscopic detection system and method under the physiological environment. The system is composed of a detector, a piezoelectric transducer, a probe control module, a phase-locked amplifier and an ultrasonic ranging module. The detector is arranged in the physiological environment to scan biological cells, and the piezoelectric transducer vibrates at ultrasonic frequency. Ultrasonic signals penetrate through the biological cells to generate different signal responses. The system acquires the response signals so as to obtain the biological cell morphology and internal maps. The ultrasonic ranging module records and processes echo delay signals and quantitatively expresses an acoustic path of a vertical direction so as to reconstruct the depth map of the biological cells. Nanoscale ultra-micro internal structural analysis of the biological cells can be realized, a probe can be accurately located and guided to inject drug loading nanoparticles into cancer cells and the stress changes of the morphology and the internal structure can be observed so that the system and the method can be used for nanoscale lossless measurement of the living cells under the physiological environment and have a directive significance for nano-biotechnology and nano-medicine.

The invention discloses a method for rapidly preparing a nano-silver bacteriostatic agent through a ligustrum quihoui fruit extracting solution, and belongs to the technical field of nano-biology. Themethod comprises the following steps that the mature ligustrum quihoui fruit is collected, the ligustrum quihoui fruit is dried to the constant weight after being thoroughly cleaned, and the ligustrum quihoui fruit is ground into powder; the ligustrum quihoui fruit powder is taken, ultra pure water is added, heating and refluxing are conducted, after natural cooling, pressure-reduction suction filtration is conducted, centrifuging is conducted, and finally the ligustrum quihoui fruit extracting solution is obtained; the ligustrum quihoui fruit extracting solution is taken, the AgNO3 solutionis added, diluting to graduation is performed by using the ultra pure water, and the constant-volume solution is arranged in an ultrasound instrument to be subjected to ultrasound treatment; and meanwhile, sunlight irradiation is conducted, and the nano-silver bacteriostatic agent is obtained. According to the method, the ligustrum quihoui fruit extractive is utilized for biologically synthesizingnano-silver, the light-catalyzed reaction with the assistance of ultrasonic waves is adopted, the synthesizing speed is obviously increased, and synthesizing can be finished within 1-15 min. The method is environment-friendly, materials are easy to obtain, the products have high uniformity and dispersibility, the product quality is stable, an obvious antibacterial effect is achieved, the antimicrobial spectrum is wide, and popularization is facilitated.

The invention relates to a construction method of an ultra-sensitive surface-enhanced Raman spectrum (SERS) sensor for measuring Hg<2+>, belonging to the technical field of nano-biotechnology detection. According to the method, gold nanoparticles with length of 15nm are respectively modified by two aptamer sequences for capturing Hg<2+> and are assembled to form chain structures in the presence of Hg<2+>, and the chain structures are measured by virtue of an SERS. By virtue of high selectivity and capturing capacity of double-stranded DNA consisting of mismatched bases of thymine-thymine (T-T) to Hg<2+>, the gold nanoparticles are assembled to form the chain structures with different lengths, and Hg<2+> is detected by virtue of SERS signals. The method is high in Hg<2+> detection sensitivity, good in specificity and high in practicability and is an ideal ultra-sensitive detection method for trace Hg<2+>.

The invention belongs to the technical field of biomedical polymer material and nanobiomaterial, particularly relates to a magnetic nano-carrier with targeted hydrophobic drug delivery to tumor and a preparation method thereof. The magnetic nano-carrier is formed by encapsulating superparamagnetic nano-particles with amphiphilic segmented copolymer methoxy polye thylene glycol-poly(lactide- glycolide) (MePEG-PLGA) micelles. First, a large amount of MePEG-PLGA with good dispersion is synthesized by the improved solution polymerization process, and then the MePEG-PLGA is used as raw materials to encapsulate Fe3O4 nano-particles by the solvent evaporation process to obtain a magnetic nano drug carrier. The carrier is expected to solve the solubility problem of insoluble drugs, achieves slow release of drugs, and achieves the passive-targeting of the drugs by the EPR effect of tumor; Fe3O4 nano-particles are used in the active-targeting of the drugs to reduce the dosage and the side effect and improve the treatment efficiency; besides, the carrier can be used in the magnetic resonance imaging (MRI) of tumor.

The invention provides a method for carrying out DNA (deoxyribose nucleic acid) detection based on PCR (polymerase chain reaction) assembled magnetic nanoparticles and belongs to the field of nanobiotechnology detection. The method comprises the following steps of: coupling magnetic nanoparticles with an upstream primer and a downstream primer respectively, assembling the magnetic nanoparticles by applying PCR, and detecting a magnetic nanoparticle assembly by adopting a magnetic resonance signal. According to the method for detecting DNA based on the magnetic nanoparticle assembly, magnetic nanoparticles coupled with primers are used, assembling in different degrees is carried out by applying PCR in presence of a target DNA, and the target DNA is detected by the magnetic resonance signal by virtue of difference of aggregation extents of magnetic nanoparticle assemblies. The method for detecting DNA based on PCR-assembled magnetic nanoparticles can realize ultrasensitive detection on DNA, detection limit is low, detection sensitivity is high, specificity is good, and high throughout detection requirement can be realized.

The invention relates to a nano-sized release, control medicinal fertilizer and its preparation method, wherein the fertilizer comprises Fusuan'an (HA30-40%, N 5%) 250kg-400kg, organic nitrogen (N 46% in ultramide) 50kg-150kg, superphosphate (P2O5 12%-20%) 100kg-250kg, potassium calcium diphosphate (35-45%) 50kg-200kg, potassium sulphate (40-55%) 100kg-150kg, powdered glauconite ore 35-52% 100kg-500kg, auxiliary addition agent (amino acids, chitosan, india quasslawood othrine, Fe, Cu, Mn, Zn, B, Mo) 6kg-12kg, and industrial water 150-300kg.

The invention discloses a method and a system for carrying out magnetic micromanipulation on a cell in a physiological environment. The system for carrying out the magnetic micromanipulation on the cell in the physiological environment comprises a magnetic probe, a probe nanometer offset detection module, a three-dimensional displacement platform, an inverted / upright optical microscope, a magnetic probe control module and a displacement control module and realizes the in-vitro operation and real-time tracking observation of a biological cell by being placed into a fine controlled culture environment suitable for the in-vitro growth of the cell. The method for carrying out the magnetic micromanipulation on the cell is characterized by measuring a single cell which contains magnetic nanometer particles point by point according to a selected area by utilizing the magnetic probe on the basis that the magnetic micromanipulation on the cell is combined with the magnetic nanometer particles, recording the magnitude of magnetic force, simultaneously acquiring the magnetic force and shape dot images at a nanometer scale and operating, moving, carrying, injecting and testing the single cell by using the magnetic probe by accurately positioning the magnetic cell and the magnetic nanometer particles, thereby achieving the guiding significance on a nanometer biological technology and micro-nanomanipulation technologies.

A composite phytoalexin containing nano-class saccharide for improving and regulating the life quality of plants contains chitosan, biomembrane assistant, phytoalexin, abscisic acid and multi-vitamine phytosaccharide. Its preparing process is also disclosed, which features use of nano technology.

The invention belongs to the field of nanometer material preparation technologies and nanobiotechnology applications and relates to a preparation method of a magnetic upconversion nanocomposite material. According to the preparation method, a DNA molecule is used as a bridge, magnetic nanoparticles are connected with rare earth doped upconversion nanoparticles by utilizing base complementary pairing action to establish a magnetic upconversion nano-composite structure, and the structure both has superparamagnetism and upconversion luminescence characteristic. By adjusting pH value of a system, raising the temperature or adding competitive DNA to remove the magnetic nanoparticles, cancellation to upconversion luminescence by the magnetic components can be eliminated and the detection sensitivity of the upconversion luminescence characteristic can be improved. The method is simple, operation is convenient and quick, and the synthesized magnetic upconversion nanocomposite material can be applied to bio-medical fields of bioseparation, bioinstrumentation, upconversion luminescence imaging, and the like.

The invention relates to a method for detecting bisphenol A (which is abbreviated as BPA) by utilizing an aptamer-based chiral sensor and belongs to the field of the detection of nano biological technology. The method comprises the following steps of modifying a DNA (deoxyribonucleic acid) probe by utilizing gold nanoparticles, assembling an asymmetric dipolymer through the gold nanoparticles, and detecting the BPA through the gold nanoparticle assembly by utilizing a circular dichroism (CD). The method has the beneficial effects that the gold nanoparticle probe modified by BPA aptamer is applied, on the basis of alkali-base complementary pairing principle, the aptamer and the gold nanoparticles modified by the complementary sequence are assembled to form the chiral dipolymer under the hybridization effect of the DNA, and the BPA is detected through the difference of CD signals under different BPA concentrations. The method is simple, fast, high in sensitivity, good in specificity and capable of detecting the real sample.

The invention belongs to the field of nanometer biological technical detection, in particular to a method for detecting pathogenic microorganism based on phage surface display peptide ligands and biomimetic nanometer material. The method comprises the following steps of using a multifunctional biological nanometer probe and to-be-detected target pathogenic microorganism to incubate, and detecting under a chromogenic system, wherein the multifunctional biological nanometer probe uses a biological screening technique to screen phage monoclone specifically combined with the pathogenic microorganism from a scenery phage library, and further separate and purify to obtain protein at the corresponding site of the phage which displays specific peptide; assembling the capsid protein of the phage displaying specific peptide onto the biomimetic nanometer material. The method has the advantages that the probe can specifically identify and combine with the target microorganism, the signal can be amplified by the biomimetic activity, and then the immune colorimetric quantitative detection of vibrio parahaemolyticus is realized; the operation is simple, the cost is low, the specificity and sensitivity are higher, and an effective path is provided for the sensitivity detection of the pathogenic microorganisms.

The invention provides a construction method of a chiral sensor for detecting DNA enzymatic assembly of a lead ion, belonging to the field of nano-biotechnology detection. The method comprises the steps of modifying a silver nano particle of 10nm and a silver nano particle of 20nm with a pair of DNA probes respectively, and assembling the modified silver nano particles to form an asymmetrical chiral dimer under the action of DNA enzyme, wherein the silver nano particle assembly is taken as a detecting sensor for detecting Pb<2+> by circular dichroism (CD). The construction method is used for detecting the Pb<2+> through a CD signal by the means that the big silver nano particle and the small silver nano particle are assembled to form the asymmetrical chiral dimmer under the action of the Pb<2+>-dependent DNA enzyme and by virtue of the silver nano particle probe. The method can be used for performing ultra-sensitive detection on the Pb<2+>, is high in detecting sensitivity and good in specificity, and is capable of detecting a trace target object in a water solution.

The invention relates to a double-labeling kit for detecting troponin (Tn) and a compound and preparation and detection methods of the double-labeling kit, and belongs to the field of immunodetectionanalysis technologies and nano-biotechnologies. The kit comprises a reaction buffer solution integrated on a reagent strip, a cleaning solution, an enhancement solution, a magnetic particle solution coating a cTnI monoclonal antibody, a europium-labeled cTnC monoclonal antibody solution and a samarium-labeled cTnI monoclonal antibody solution and 2-6 bottles of additional freeze-dried calibrationproducts of natural cTnI antigens (containing cTnC) with different concentrations. The kit can provide a nearly-homogeneous double-labeling reaction system to ensure that the content of cTnI with complete fragments and the cTnI-C compound is detected simultaneously, the detection time is shortened, the efficiency is improved, and convenience is brought to clinical use.

The invention relates to a nanometer type natural biological protein bee products and manufacturing method thereof. It adopts the nanometer biological technology structure process, extracts and utilizes the pharmacody properties of the drug in the largest extent and optimize combines the supercritical extracted and purified biological active effective constituent. It has micro molecule effects for control releasing and targeting, and has duplex functions of healthy care and diet therapy for inhibiting the brain atrophy, high blood fat and diabetes. It provides a novel protection and treatment measures for lowering the blood sugar and blood fat, reversing the canceration. The invention comprises following constituents: propolis 5-50b, royal jelly 10-45b, bee pollen 15-40b, ganoderma lucidum 8-25b, gingko leaf 10-20b, aloe 8-25b, nervonic acid 4-15b, chitosan 4-10b, maple leaf 4-10b, honey suckle 10-15b, licorice 8-15b, hawthorn fruit 8-15b.(b is a weight unit)

The invention relates to a double-tagging time resolution fluoroimmunoassay reagent kit based on a PG magnetic particle and a preparing method and detecting method thereof and belongs to the technical field of immune detection analyzing techniques and nanometer biological techniques. The reagent kit comprises a reaction buffer solution, a concentrated cleaning solution, an enhancing solution, a magnetic particle solution coated with PGI monoclonal antibody, a magnetic particle solution coated with PGI monoclonal antibody, a PG calibrator solution, an europium-labeled PGII monoclonal antibody solution and a samarium-labeled PGI monoclonal antibody solution which are independently packaged. The reagent kit can provide a double-tagging reaction system close to a homogeneous phase, can guarantee that the content of PGI and the content of PGII can be detected at the same time, enables a detecting result of PGI and PGII in the same sample liquid to be high in accuracy and small in error, greatly shortens reaction time, shortens detection time, improves efficiency, improves detection sensitivity and specificity and brings great convenience to clinical application.

The invention belongs to the field of a nano-biotechnology and particularly relates to a method for marking immune globulin by a quantum dot. The method comprises the following steps: washing the quantum dot, activating, washing the quantum dot for the second time, washing the immune globulin, coupling and separating. According to the method, structural characteristics of the water-soluble quantum dot, the reaction characteristics of EDC (1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) activated carboxyl and the reaction characteristics of a condensation reaction are utilized; the carboxyl on the surface of the quantum dot is activated sufficiently by optimizing conditions of all the steps and is in a suitable marking environment; the quantum dot can be combined with the surface of the immune globulin by the condensation reaction through optimizing the condition of the coupling step, and the marking efficiency is high. The immune globulin which is not reacted can be recycled to be marked continually through exclusion chromatography, separation and purification by a reaction solution obtained by the coupling step so that the utilization rate of the immune globulin is improved and the cost is saved. According to the method provided by the invention, the activity of a prepared protein-quantum dot conjugate is high, the operation and used equipment are simple, and the production can be enlarged industrially.

The invention belongs to the technical field of nanobiology, particularly relating to a preparation method of a magnetic separation fluorescence quantitative detection kit of aflatoxin B1. The invention is a measuring method which combines the quick separation technology of an immunological detection system and magnetic nanometer material with the lightening characteristics. Firstly, magnetic isolating reagent, namely compound (I) is prepared; then, aflatoxin B1 is coupled to the compound (I); fluorescent particle solution (compound (II)) coupled with holoantigen is added; the aflatoxin B1 in the compound (II) and residual antibody on the compound (I) generate immune response to form the immunological sandwich compound (III) of the compound (I) and compound (II); and thus, the density of aflatoxin is in inverse proportion to the fluorescence intensity of the fluorescence particle solution of the final detected solution. The invention has the advantages of quick, accurate and obvious detection, high sensitivity and the like.

The invention provides a peroxide nanoenzyme and a preparation method thereof, belonging to the technical field of nano-biotechnology. According to the method, a gold nanoparticle is used as a carrier, biologically active pentapeptide (URGDC) is employed for self-assembly, and a mercapto group in a pentapeptide and the gold nanoparticle undergo coordination and covalent coupling so as to obtain the peroxide nanoenzyme with high GPx activity. The method is simple to operate and has good stability. The amino acid sequence of the biologically active pentapeptide is Sec-Arg-Gly-Asp-Cys (referred as URGDC for short), wherein Sec(U) contains the active center of the nanoenzyme, Arg-Gly-Asp (RGD) can promote bonding between the nanoenzyme and a substrate, and the mercapto group in a Cys (C) side chain can realize covalent connection with the gold nanoparticle. The gold nanoparticle is used as a carrier for immobilizing the biologically active pentapeptide, promotes full contact between the nanoenzyme and the substrate, and improves activity of glutathion peroxidase.

The invention discloses a method for labeling protein through quantum dots and belongs to the field of nano-biotechnology. The method is characterized by expressing a Histag on protein, obtaining a protein dimer containing two Histags through protein dimerization, mixing the protein with the quantum dots according to a certain ratio, and oscillating to enable the two Histags to combine with the quantum dots simultaneously so as to improve the stability of combination. The method is simple in operation, and the quantum dots can be applied to biomarker widely as a nano fluorescent probe.

The preparation method for chiral separating stationary phase with functional nano carbon tube bonded protein as microflow chip overcomes the defect of bad effect and unstable performance led by unstable chip electroosmosis, can be applied to modified chip effectively by means of the fixed phase in chip separation channel, and has very high success rate. Wherein, the fixed protein has high activity and well stability.

A nano-class hard hollow capsule is prepared from refined starch and active protein through mixing them with modifier, thermodecomposing reaction, washing, drying, superfine treating (including thermomodifying), regulating pH value, adding cross-linking agent, preparing sol, adding coagulant and coagulating aid, stirring, dipping resin by template, baking, pulling shell, cutting and finishing.

The invention discloses a preparation method of a nanomaterial for preventing decayed teeth and treating superficial caries, and relates to the fields of nano-biotechnologies and oral care technologies. The preparation method comprises the following steps: uniformly dispersing polydopamine particles in an aqueous solution system, adding an aqueous solution containing silver ions under a magnetic stirring condition, carrying out a reaction for a period of time, performing high-speed centrifuging, discarding the obtained supernatant, and washing the obtained reaction product with deionized water; and slowly adding an aqueous solution of molecules having the ability to recognize dental enamel to a high-speed stirred catalyst system solution, carrying out a reaction for a period of time, slowly adding the prepared antibacterial component-immobilized core aqueous solution to the obtained high-speed stirred solution system, carrying out a reaction for a period of time, performing high-speedcentrifugation, discarding the obtained supernatant, washing the obtained reaction product with deionized water to obtain the nanomaterial. The nanomaterial prepared by the preparation method is formed by the inner nanoparticle core having an induced mineralization effect, and an external containing the above broad-spectrum antibacterial component and the dental enamel recognition molecules.

The invention belongs to the technical field of nanometer biology, and specifically discloses a nucleic acid aptamer-suspension chip based on single-walled carbon nano-tubes, a preparation method and a detection method thereof. According to the present invention, with the application of the nucleic acid aptamer-suspension chip based on single-walled carbon nano-tubes to detect a target substance, the detected median fluorescence signal intensity is negatively correlated with the concentration of the substance to be detected, and the concentration of the substance to be detected in the sample to be detected can be obtained by establishing the standard curve between the concentration of the substance to be detected and the fluorescence signal; and the detection method has advantages of wide detection range, high detection sensitivity and high specificity, and has great advantages compared to other detection techniques.

The invention belongs to the nano-biotechnology and a nano-catalysis aquaponics method. The existing aquaponics technology has the disadvantages that the fish excrement decomposition speed is low, aquaponics starting time is long and production efficiency is low. The method includes the steps of preparing aquaponics nano-catalysts, smashing tourmaline, medical stone and zeolite till the density of meshes reaches 2500, accurately weighing purple grit mud, tourmaline, nanometer titania, nanometer magnesia, medical stone and zeolite, mixing all the compositions, pressing the mixture into a grain shape or a tablet shape with water content of 20%, sintering the mixture at a temperature of 1100 DEG C to 1200 DEG C for 10 hours, placing the nano-catalysts and aquaponics microbial strain bacillus into a fish breeding water body, pumping out fish breeding pool water to be used for vegetable planting, making water absorbed by vegetables flow back into the fish breeding water body, and repeating the circulating. The nano-catalysis aquaponics method has the advantages of being high in catalysis speed, quick in fish excrement decomposition, and capable of shortening the aquaponics starting time, saving resources and improving the aquaponics production efficiency.

The present disclosure is in relation to nanotechnology / nanobiotechnology, in particular to nano, meso and micro structures of Naphthalene diimide derivatives. The disclosure provides a method for supramolecular self-assembling of Naphthalene diimide derivatives, its characteristics and its applications. The present disclosure also relates to self assembled nano, meso or microstructures of the Naphthalene diimide derivatives.

The invention discloses a novel annular polypeptide ligand (ATTO-H6) used for modifying quantum dots, and belongs to the technical field of nano-biology. The novel annular polypeptide ligand comprises a dye ATTO 590 (1) used for undergoing FRET together with quantum dots, a sequence (2) used for polypeptide cyclization, histidine intervening sequences (3) used for combining the quantum dots, and sequences (4) used for polypeptide folding, and all sequences are combined through peptide bonds. The ligand is simple to synthesize, and the combination of six histidine intervening sequences and the quantum dots greatly improves the combination rate and stability of the ligand and the quantum dots, so the ligand can be widely used in biological marking as a fluorescence nano-probe.

The invention relates to a double-tagging time resolution fluoroimmunoassay reagent kit based on a PSA magnetic particle and a preparing method and detecting method thereof and belongs to the technical field of immune detection analyzing techniques and nanometer biological techniques. The reagent kit comprises a reaction buffer solution, a concentrated cleaning solution, an enhancing solution, a PSA monoclonal antibody coated magnetic particle solution, a PSA calibrator solution, an europium-labeled fPSA monoclonal antibody solution and a samarium-labeled tPSA monoclonal antibody solution which are independently packaged. The reagent kit can provide a double-tagging reaction system close to a homogeneous phase, can guarantee that the content of tPSA and the content of fPSA can be detected at the same time, enables a detecting result of tPSA and fPSA in the same sample liquid to be high in accuracy and small in error, greatly shortens reaction time, shortens detection time, improves efficiency, improves detection sensitivity and specificity and brings great convenience to clinical application.

The vaccine preparing process includes the steps of: analyzing the specific antigen of specific pathogen; obtaining the polynucleotide sequence encoding the specific antigen; obtaining polynucleotide sequence with sufficient difference from the polynucleotide sequence; and utilizing the polynucleotide sequence obtained in the last step to prepare vaccine. The present invention also provides EGFR molecular vaccine as an antitumor vaccine, and the EGFR molecular vaccine may be conventional preparation, such as aqua preparation, or nano preparation. EGFR molecular vaccine has antitumor effect capable of being strengthened under the synergistic effect of other immunity promoting factor.

The invention belongs to the field of the nanometer biological technology application, and discloses a troponin I autoantibody detection method based on gold nanoplates. The method includes the following steps: coating a cardiac troponin I antigen on the gold nanoplate, detecting gold nanoplates with a spectrometer using specific binding between the cardiac troponin I antigen coated on the gold nanoplate and a cardiac troponin I autoantibody in a sample to be measured, thereby realizing the quantitative determination of the cardiac troponin I autoantibody. The detection method has the advantages of good stability, repeatability and simple operation, and a new approach of gold nanoplate is developed in the field of biomedical science.