15 results about "Nanobiotechnology" patented technology

The application discloses silica nano composite fertilizer, a preparation method and application thereof, and belongs to the field of nanobiotechnology. The silica nano composite fertilizer is prepared by taking modified dendritic mesoporous silica as a carrier and loading ionic compounds. The silica nanoparticles prepared by the application have excellent nanometer size, and the formed nano organic fertilizer can better help the transportation of element ions in plants. After modification, the loading and penetration guiding effect of the silica nanoparticles is improved, so that the yield and quality of vegetables are improved, the transportation performance of various elements in the vegetables is improved, and the utilization rate of element fertilizers is improved.

This invention is related to the fields of medical biotechnology, nanobiotechnology, and nanomedicine. It involves a nanomedicine product that can release HOC1 (hypochlorous acid) at the tumor site in a controlled manner to treat lung cancer. HOC1 is a potent antimicrobial and antitumor agent that can target tumor tissues while minimizing damage to healthy tissues. The produced nanoparticles have a two-layer structure: the inner layer contains alginate, PFP (perfluoropentane), and Ca(OCl)2 to release HOC1, and the outer layer has a chitosan coating with folate and salicylic acid for targeting and proton release. The production process involves nanoemulsion and coating with the ionic gelation technique. When the nanoparticles are delivered to the lungs and targeted to cancer cells with folate, ultrasound radiation triggers the release of HOC1 to induce apoptosis and destroy cancer cells effectively, reducing side effects and improving treatment outcomes for lung cancer patients.

This invention discloses a multi-stimulus-responsive upconversion luminescent nanocrystalline magnetic microsphere and its preparation method, belonging to the field of biomedical polymer materials and nanobiotechnology. The microsphere's main body is a porous copolymer matrix, encapsulating a core of magnetite (Fe3O4) magnetic nanocrystals. Rare-earth-doped fluoride upconversion luminescent nanocrystals are coupled to the surface and pores. The copolymer side chains of the microsphere incorporate azide groups, acidic stimulus-responsive groups, and photostimulation-responsive groups, constructing an independent and non-interfering triple orthogonal logic gating system on the surface. The preparation method includes preparing an oil phase containing copolymers, magnetic nanoparticles, and a volatile porogen; pre-emulsifying and homogenizing the oil phase in an acidic aqueous phase using an SPG membrane; inducing phase separation by controlled solvent evaporation to form porous microspheres; and finally coupling the upconversion nanocrystals via a click chemistry reaction. The magnetic microspheres of this invention eliminate background fluorescence interference from complex samples from a physical perspective. Their porous structure endows the microspheres with excellent quasi-levitation properties and magnetic responsiveness, effectively solving problems such as single surface function and signal crosstalk of microspheres. They are suitable for in vitro clinical diagnosis and complex analysis of multiple targets.

The invention belongs to the technical field of nano biology, and particularly discloses sea-buckthorn-derived extracellular vesicles and a preparation method and application thereof.The preparation method comprises the following steps that sea-buckthorn raw pulp is taken and centrifuged, and an upper-layer oil phase is removed; centrifuging the absorbed middle-layer water phase, and removing an upper-layer oil phase; absorbing a middle-layer water phase and centrifuging; removing an upper-layer oil phase and a middle-layer water phase, fully resuspending the precipitate by using a buffer solution, centrifuging, retaining the precipitate, and fully resuspending by using the buffer solution; and centrifuging the resuspended turbid liquid, discarding the supernate, resuspending the precipitate by using a buffer solution, and centrifuging the resuspended solution, thereby obtaining the solution of the sea-buckthorn-derived extracellular vesicles. According to the sea-buckthorn-derived extracellular vesicle as well as the preparation method and the application thereof, the complete structure and the biological activity of the extracellular vesicle are reserved. Meanwhile, the application of the extracellular vesicle in improvement of mitochondrial dysfunction and anti-aging is defined, and a material basis and technical support are provided for developing novel anti-aging drugs.

The invention discloses a method for preparing a high-interfacial-activity phospholipid-olein nano-composite, and belongs to the crossing field of a food technology, a colloid interface chemical technology and a nano-biotechnology. The method comprises two steps of co-dissolution of phospholipid and oleosin and self-assembly of phospholipid and oleosin: dissolving 5mg of phospholipid with the saturation degree of 0-2 in 10mL of distilled water, performing vortex for 3 minutes, then performing ultrasonic treatment for 30-90 seconds under the power of 200-400W to obtain a phospholipid stock solution, then mixing 1mg of oleosin solid powder, 2mL of phospholipid stock solution and 8mL of distilled water, performing vortex for 5 minutes, and performing centrifugal separation to obtain the phospholipid and oleosin self-assembly. The preparation method comprises the following steps: carrying out ultrasonic treatment for 30-90 seconds at the temperature of 25 DEG C, standing for 1-3 hours at the power of 200-400 W, then centrifuging for 5-30 minutes at the speed of 3000-5000 rpm, and collecting supernate obtained after centrifuging, namely the high-interfacial-activity phospholipid-oleosin nano-composite. The particle size of the prepared high-interfacial-activity phospholipid-olein nano-composite ranges from 200 nm to 600 nm, and interfacial tension can be effectively reduced when the high-interfacial-activity phospholipid-olein nano-composite is adsorbed to an oil-water interface.

This invention provides a composition containing Dendrobium exosomes, its preparation method, and its application, belonging to the field of nanobiotechnology. This invention efficiently obtains highly active Dendrobium exosomes through a combination of differential centrifugation and ultracentrifugation, and innovatively combines them with specific proportions of moisturizing agents such as dipropylene glycol and erythritol, and anti-inflammatory components such as Centella asiatica extract, forming a composition system that can be stably stored at room temperature. This composition not only effectively protects the activity of exosomes and solves their storage problems, but also utilizes the natural transmembrane transport function of exosomes to synergistically enhance the penetration of small molecule active ingredients into the skin, achieving multiple synergistic effects of whitening, moisturizing, anti-oxidation, and anti-inflammation. This provides a new strategy for developing plant exosome cosmetics that are both highly effective and practical.

The application belongs to the field of nanobiotechnology, and discloses a metal organic framework peroxidase nanoenzyme, a preparation method and application thereof. The metal organic framework peroxidase nanoenzyme is prepared by using azole compounds as organic ligands, iron series elements as metal centers, and a mixture of deionized water and organic solvents in a certain proportion as a solvent. After mixing a metal ion solution and an organic ligand solution at room temperature, the metal organic framework peroxidase nanoenzyme is formed by reaction and coordination assembly through a solvothermal method. By adjusting the proportion of water and organic solvents in the preparation process, the metal organic framework peroxidase nanoenzyme with an optimal pH of neutral or near-neutral conditions is constructed. The nanoenzyme preparation method is simple and controllable, low in cost, and easy to synthesize in large quantities, thereby providing a train of thought for the design, synthesis and application of new MOF nanoenzymes.

The invention belongs to the technical field of nano biology, and particularly discloses sea-buckthorn nano vesicles and an extraction method and application thereof.The extraction method comprises the following steps that sea-buckthorn raw pulp is taken and placed in a sterile centrifugal tube to be centrifuged, and an upper-layer oil phase is removed; absorbing a middle-layer water phase, centrifuging, and removing an upper-layer oil phase; absorbing a middle-layer water phase and centrifuging; discarding a small amount of oil phase at the upper layer, discarding a water phase at the middle layer, fully resuspending the precipitate, centrifuging, retaining the precipitate, and fully resuspending; and centrifuging the resuspended turbid liquid, discarding the supernate, resuspending and precipitating, centrifuging the resuspended solution, and taking the light yellow transparent liquid which is the PBS solution of the sea-buckthorn nano vesicles. According to the sea-buckthorn nano-vesicle as well as the extraction method and the application thereof, the complete structure and the biological activity of the extracellular vesicle are reserved. The application of the vesicles in medicines for promoting skin injury repair is defined, so that the extraction problem of extracellular vesicles in grease-rich plants is solved, and novel effective components are provided for skin injury treatment.

The invention relates to the technical field of biological pharmacy and nano biology, and provides mRNA-lipid nanoparticles targeting macrophages, a preparation method of the mRNA-lipid nanoparticles and application of the mRNA-lipid nanoparticles in preparation of drugs for treating cardiovascular diseases. The surface of LNP is modified by folic acid (FA) modified polyethylene glycol (PEG) lipid (FA-PEG-2000), so that specific targeting of macrophages is realized, mRNA (mod-mRNA) of a burial regulatory factor RAB11FIP1 is entrapped, and the macrophages are used for enhancing the burial effect of the macrophages, inhibiting pro-inflammatory phenotypic polarization and promoting repairable phenotypic transformation, thereby alleviating cardiac inflammatory response and improving prognosis of cardiovascular diseases. Compared with the prior art, the macrophages are effectively targeted, heart recruitment of inflammatory cells is achieved, the intercellular effect and the anti-inflammatory effect of the macrophages are enhanced, inflammation immunity is targeted, and a new treatment strategy is provided for treatment of cardiovascular diseases.

The application belongs to the field of nanobiotechnology, and discloses a super-small size cerium metal organic framework nanometer enzyme and a preparation method and application thereof. The nanometer enzyme is prepared by rapid self-assembly of cerium ions and azole ligands in an aqueous phase at room temperature. The catalyst is regulated by MOF nanoparticles, and the catalytic activity is enhanced. Compared with large size Ce-MOF, the super-small size (preferably 5-10 nm) Ce-MOF nanometer enzyme has more defect structures and dense active sites. Compared with natural enzymes, the Ce-MOF nanometer enzyme has the characteristics of simple synthesis, high yield, good stability, strong tolerance to extreme environments, high enzyme-like catalytic activity, and the like, and can complete the hydrolysis of organophosphorus pesticides, organic nerve toxic agents and phosphate biomolecules. The nanometer enzyme has high activity utilization rate in recycling, can adapt to various phosphate compounds, and provides an efficient catalyst for environmental treatment and biomedical treatment applications.

The invention relates to the field of nano biotechnology and medical detection, and particularly discloses a synthesis method and application of a lipid nanoparticle (LNP) mRNA molecular probe based on a 31P magnetic resonance spectrum (MRS). According to the probe, mRNA is entrapped by ionizable lipid, DSPC, cholesterol and DMG-PEG2000, the ratio and parameters are optimized by adopting a microfluidic technology, and LNP with uniform particle size and stable structure is prepared. According to the method, an mRNA phosphate skeleton is used as a 31P MRS signal source, Triton X-100 and R Nase A are used for processing and simulating release and splitting decomposition states of mRNA-LNP in vivo, and a 400-600MHz nuclear magnetic resonance spectrometer is used for detecting a phosphorus spectrum signal. Based on 31P signal intensity and chemical shift changes in different states, visual identification of the in-vivo metabolic state of mRNA-LNP is realized. The probe does not need additional marking, is simple to operate and good in repeatability, and has relatively high detection sensitivity and biological adaptability.

The invention belongs to the technical field of crossing of medical instruments and nanometer biotechnology, and particularly relates to a nanometer enzyme functionalized acupuncture needle and a preparation method and application thereof.The surface of a needle body of the acupuncture needle is fixedly provided with palladium nanoparticles through a polydopamine adhesion layer, and a composite coating is formed; the palladium nanoparticles have catalase-like and superoxide dismutase-like activities, and are used as carriers to load hydrogen and a therapeutic drug tolbuzumab. The preparation method comprises the steps of polydopamine coating deposition, palladium nanoparticle in-situ reduction, hydrogen loading and drug loading. When electrical stimulation is applied, the acupuncture needle can synergistically enhance enzyme activity, controllably release hydrogen and drugs and regulate and control pyroptosis of cells in a multi-channel mode, so that the acupuncture needle is used for preparing medical instruments for treating rheumatoid arthritis and has the advantages of being high in local effect and small in system side effect.

The invention discloses a physical unclonable anti-counterfeiting material based on a DNA (Deoxyribose Nucleic Acid) nanostructure as well as a preparation method and application of the physical unclonable anti-counterfeiting material, and belongs to the technical field of information security and nano biology. According to the anti-counterfeiting material, three regular tetrahedron DNA monomers are connected through flexible double chains to form a DNA tripolymer, double-chain thermodynamic flexibility endows the anti-counterfeiting material with a nanoscale random geometric configuration, multi-dimensional encryption is achieved through different spectrum fluorophores modified randomly, and the information entropy reaches 4.3 bits / pixel. By combining deterministic self-assembly of the DNA nano-structure with dynamic flexibility, unification of deterministic preparation and random anti-counterfeiting characteristics is realized, and the method has excellent biocompatibility, high safety and strong expansibility, can be seamlessly compatible with a DNA computing system and a data storage system, and has wide application prospects. Multiple scenes such as information encryption authentication, biomedical authentication, flexible electronic anti-counterfeiting and DNA storage chip anti-counterfeiting are adapted, and an innovative platform is provided for the next generation of security authentication technology.

The application discloses a kind of defective metal organic framework antioxidant nanoenzymes and preparation method and application thereof, belong to nanobiotechnology field.The preparation method is that copper source, zirconium source and organic ligand containing pyrazole and carboxyl are dissolved in organic solvent, then competitive ligand is added, after being mixed uniformly, solvent thermal reaction is carried out at 80 DEG C~120 DEG C, and defective metal organic framework is prepared;Defective metal organic framework aqueous solution and kidney targeting molecule solution are mixed uniformly, and through coordination and electrostatic adsorption, defective metal organic framework antioxidant nanoenzyme is obtained.The application is prepared by adjusting particle size and defect by organic ligand containing pyrazole and carboxyl, competitive ligand containing carboxylic acid and organic solvent, and metal organic framework with small particle size and containing defect is prepared, antioxidant activity is improved, and kidney targeting molecule is loaded to obtain defective metal organic framework antioxidant nanoenzyme, to realize the targeting effect of kidney.

The invention discloses an ochrobactrum strain capable of efficiently tolerating selenate and biologically synthesizing nano-selenium and application of the ochrobactrum strain, and belongs to the technical field of microbial fermentation and nano-biology. The screened ochrobactrum strain which is efficient, selenium-resistant and capable of biologically synthesizing nano-selenium is classified and named as Ochrobactrum sp. RS1 and is preserved in the China Center for Type Culture Collection on December 15, 2025, the preservation date is December 15, 2025, and the preservation number is CCTCC NO: M20252876. After the strain is subjected to selenium-resistant domestication, the highest tolerance concentration of the strain to sodium selenite can reach 300 nM, the sodium selenite can be reduced into uniform spherical nano-selenium particles in a liquid culture medium, and the average particle size is 190.0-282.5 nm. By optimizing fermentation conditions (PH, temperature and culture time), the conversion rate of the domesticated ochrobactrum anthropi RS1 to sodium selenite reaches 70.20% + / -0.39%. Compared with a traditional chemical and physical nano-selenium synthesis method, the production efficiency of nano-selenium is improved by fermenting sodium selenite with ochrobactrum anthropi RS1, the production cost is reduced, and the method has the advantages of being environmentally friendly, easy and convenient to operate and the like.