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Double-tagging time resolution fluoroimmunoassay reagent kit based on PG magnetic particle

A time-resolved fluorescence and immunoassay technology, applied in the field of immunoassay analysis technology and nanobiology, can solve the problems of large error of PGI/PGII ratio, low efficiency, unsuitable for sample screening, etc.

Inactive Publication Date: 2016-07-20
JIANGSU INST OF NUCLEAR MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved in the present invention is that PGI and PGII cannot be detected simultaneously in the prior art, the calculated PGI / PGII ratio error is large, the accuracy of the detection result is low, and the detection time is long, the sensitivity and specificity are poor, and the efficiency is low. It is not suitable for a large number of sample screening, and then provide a double-labeled time-resolved fluorescence immunoassay kit and its detection based on PG magnetic particles that can simultaneously detect PGI and PGII, with accurate and reliable detection results, high sensitivity, high specificity and short detection time method and use

Method used

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  • Double-tagging time resolution fluoroimmunoassay reagent kit based on PG magnetic particle
  • Double-tagging time resolution fluoroimmunoassay reagent kit based on PG magnetic particle
  • Double-tagging time resolution fluoroimmunoassay reagent kit based on PG magnetic particle

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Embodiment 1

[0075] This embodiment provides a dual-labeled time-resolved fluorescence immunoassay kit based on PG magnetic particles, including individually packaged reaction buffer, concentrated cleaning solution, enhancement solution, magnetic particle solution coated with PGI monoclonal antibody, and package Magnetic particle solution of PGII monoclonal antibody, PG calibrator solution, europium-labeled PGII monoclonal antibody solution, and samarium-labeled PGI monoclonal antibody solution, where:

[0076] The europium-labeled PGII monoclonal antibody solution is Eu 3+ -N 2 -[P-isocyanic acid-benzyl]-diethylenetriaminetetraacetate-labeled PGII monoclonal antibody is prepared, and the PGII monoclonal antibody is compatible with the Eu 3+ -N 2 -The mass ratio of [P-isocyanate-benzyl]-diethylenetriaminetetraacetate sodium is 5:1;

[0077] The samarium-labeled PGI monoclonal antibody solution is Sm 3+ -N 2 -[P-Isocyanic acid-benzyl]-diethylenetriaminetetraacetate labeled PGI monoclonal antibody...

Embodiment 2

[0103] This embodiment provides a dual-labeled time-resolved fluorescence immunoassay kit based on PG magnetic particles, including individually packaged reaction buffer, concentrated cleaning solution, enhancement solution, magnetic particle solution coated with PGI monoclonal antibody, and package Magnetic particle solution of PGII monoclonal antibody, PG calibrator solution, europium-labeled PGII monoclonal antibody solution, and samarium-labeled PGI monoclonal antibody solution, where:

[0104] The europium-labeled PGII monoclonal antibody solution is Eu 3+ -N 2 -[P-isocyanic acid-benzyl]-diethylenetriaminetetraacetate-labeled PGII monoclonal antibody is prepared, and the PGII monoclonal antibody is compatible with the Eu 3+ -N 2 -The mass ratio of [P-isocyanate-benzyl]-diethylenetriaminetetraacetate sodium is 5:1;

[0105] The samarium-labeled PGI monoclonal antibody solution is Sm 3+ -N 2 -[P-Isocyanic acid-benzyl]-diethylenetriaminetetraacetate labeled PGI monoclonal antibody...

Embodiment 3

[0131] This embodiment provides a dual-labeled time-resolved fluorescence immunoassay kit based on PG magnetic particles, including individually packaged reaction buffer, concentrated cleaning solution, enhancement solution, magnetic particle solution coated with PGI monoclonal antibody, and package Magnetic particle solution of PGII monoclonal antibody, PG calibrator solution, europium-labeled PGII monoclonal antibody solution, and samarium-labeled PGI monoclonal antibody solution, where:

[0132] The europium-labeled PGII monoclonal antibody solution is Eu 3+ -N 2 -[P-isocyanic acid-benzyl]-diethylenetriaminetetraacetate-labeled PGII monoclonal antibody is prepared, and the PGII monoclonal antibody is compatible with the Eu 3+ -N 2 -The mass ratio of [P-isocyanate-benzyl]-diethylenetriaminetetraacetate sodium is 5:1;

[0133] The samarium-labeled PGI monoclonal antibody solution is Sm 3+ -N 2 -[P-Isocyanic acid-benzyl]-diethylenetriaminetetraacetate labeled PGI monoclonal antibody...

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Abstract

The invention relates to a double-tagging time resolution fluoroimmunoassay reagent kit based on a PG magnetic particle and a preparing method and detecting method thereof and belongs to the technical field of immune detection analyzing techniques and nanometer biological techniques. The reagent kit comprises a reaction buffer solution, a concentrated cleaning solution, an enhancing solution, a magnetic particle solution coated with PGI monoclonal antibody, a magnetic particle solution coated with PGI monoclonal antibody, a PG calibrator solution, an europium-labeled PGII monoclonal antibody solution and a samarium-labeled PGI monoclonal antibody solution which are independently packaged. The reagent kit can provide a double-tagging reaction system close to a homogeneous phase, can guarantee that the content of PGI and the content of PGII can be detected at the same time, enables a detecting result of PGI and PGII in the same sample liquid to be high in accuracy and small in error, greatly shortens reaction time, shortens detection time, improves efficiency, improves detection sensitivity and specificity and brings great convenience to clinical application.

Description

Technical field [0001] The invention belongs to the fields of immunodetection analysis technology and nano-biotechnology, and specifically relates to a dual-label time-resolved fluorescence immunoassay kit based on PG magnetic particles, and a preparation method and a detection method thereof. Background technique [0002] Pepsinogen (PG) is the inactive precursor of pepsin in gastric juice. Human pepsinogen can be divided into two pepsinogen groups with different biochemical and immunological activity characteristics: pepsinogen I (referred to as PG I) and stomach Proteaseogen II (referred to as PGII), PGI is mainly secreted by the main cells of the fundus glands and cervical mucus cells, most of which enter the gastric cavity, and a small amount enters the blood circulation. PGII comes from the whole stomach gland and Brunner's gland of the distal duodenum. PGII synthesized by the gastric mucosa is about 25% of the total. Most of PGII enters the digestive tract, and a small am...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/573G01N33/543
CPCG01N33/577G01N33/54326G01N33/54346G01N33/573G01N2333/96477G01N2800/06
Inventor 黄飚范俊周彬张艺张珏郭明明邓黎莉朱岚
Owner JIANGSU INST OF NUCLEAR MEDICINE
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