Double-tagging time resolution fluoroimmunoassay reagent kit based on PG magnetic particle

Abstract

The invention relates to a double-tagging time resolution fluoroimmunoassay reagent kit based on a PG magnetic particle and a preparing method and detecting method thereof and belongs to the technical field of immune detection analyzing techniques and nanometer biological techniques. The reagent kit comprises a reaction buffer solution, a concentrated cleaning solution, an enhancing solution, a magnetic particle solution coated with PGI monoclonal antibody, a magnetic particle solution coated with PGI monoclonal antibody, a PG calibrator solution, an europium-labeled PGII monoclonal antibody solution and a samarium-labeled PGI monoclonal antibody solution which are independently packaged. The reagent kit can provide a double-tagging reaction system close to a homogeneous phase, can guarantee that the content of PGI and the content of PGII can be detected at the same time, enables a detecting result of PGI and PGII in the same sample liquid to be high in accuracy and small in error, greatly shortens reaction time, shortens detection time, improves efficiency, improves detection sensitivity and specificity and brings great convenience to clinical application.

Description

Technical field [0001] The invention belongs to the fields of immunodetection analysis technology and nano-biotechnology, and specifically relates to a dual-label time-resolved fluorescence immunoassay kit based on PG magnetic particles, and a preparation method and a detection method thereof. Background technique [0002] Pepsinogen (PG) is the inactive precursor of pepsin in gastric juice. Human pepsinogen can be divided into two pepsinogen groups with different biochemical and immunological activity characteristics: pepsinogen I (referred to as PG I) and stomach Proteaseogen II (referred to as PGII), PGI is mainly secreted by the main cells of the fundus glands and cervical mucus cells, most of which enter the gastric cavity, and a small amount enters the blood circulation. PGII comes from the whole stomach gland and Brunner's gland of the distal duodenum. PGII synthesized by the gastric mucosa is about 25% of the total. Most of PGII enters the digestive tract, and a small am...

AI Technical Summary

Problems solved by technology

[0006] Therefore, the technical problem to be solved in the present invention is that PGI and PGII cannot be detected simultaneously in the prior art, the calculated PGI/PGII ratio error is large, the accuracy of the detection result is low, and the detection time is long, the sensitivity and specificity are poor, and the efficiency is low. ...

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