This invention relates to the field of biodetection technology, providing an
enzyme cascade reaction system for the detection of nucleic acids and
protein molecules, comprising BER
endonuclease and
Argonaute endonuclease; wherein the BER
endonuclease is one or more selected from APE1, FEN1, Fpg, UDG, and T7endonuclease, and the
Argonaute endonuclease is one or more selected from pfAgo, CpAgo, CbAgo, TtAgo, RasAgo, MjAgo, and NgAgo. This invention also provides a method for detecting nucleic acids and
protein molecules using the above
system, and the application of the above
system in the preparation of target
nucleic acid and
target protein molecule products from detection samples. This invention utilizes the synergistic
enzyme activity of APE1 and PfAgo in the
enzyme cascade reaction system to achieve real-time detection of
nucleic acid and
protein biomarkers without pre-amplification within 45 minutes.