83results about How to "Realize instant detection" patented technology

The invention relates to a double-tagging time resolution fluoroimmunoassay reagent kit based on a PG magnetic particle and a preparing method and detecting method thereof and belongs to the technical field of immune detection analyzing techniques and nanometer biological techniques. The reagent kit comprises a reaction buffer solution, a concentrated cleaning solution, an enhancing solution, a magnetic particle solution coated with PGI monoclonal antibody, a magnetic particle solution coated with PGI monoclonal antibody, a PG calibrator solution, an europium-labeled PGII monoclonal antibody solution and a samarium-labeled PGI monoclonal antibody solution which are independently packaged. The reagent kit can provide a double-tagging reaction system close to a homogeneous phase, can guarantee that the content of PGI and the content of PGII can be detected at the same time, enables a detecting result of PGI and PGII in the same sample liquid to be high in accuracy and small in error, greatly shortens reaction time, shortens detection time, improves efficiency, improves detection sensitivity and specificity and brings great convenience to clinical application.

The invention provides a time resolution fluorescence immunoassay method based on magnetic separation; the method mainly comprises the following steps of firstly, coupling magnetic beads with an antibody to form immunomagnetic beads; meanwhile, enabling time resolution fluorescent microsphere to be coupled with antibody to form immunofluorescent microspheres; then enabling the immunomagnetic beadsand the immunofluorescent microspheres and the antigen in a sample to be oscillated and incubated in a reaction tube to form an immunomagnetic bead-antigen-immunofluorescent microsphere compound; andfinally, testing the fluorescence intensity, emitted by excitation of the compound at 360 nm excitation, by a time resolution instrument, wherein a standard curve is used as reference for determiningthe amount of the antigen in the sample. According to the analysis method, the reaction time is greatly shortened, and the detection efficiency and sensitivity are improved.

The invention relates to a double-tagging time resolution fluoroimmunoassay reagent kit based on a PSA magnetic particle and a preparing method and detecting method thereof and belongs to the technical field of immune detection analyzing techniques and nanometer biological techniques. The reagent kit comprises a reaction buffer solution, a concentrated cleaning solution, an enhancing solution, a PSA monoclonal antibody coated magnetic particle solution, a PSA calibrator solution, an europium-labeled fPSA monoclonal antibody solution and a samarium-labeled tPSA monoclonal antibody solution which are independently packaged. The reagent kit can provide a double-tagging reaction system close to a homogeneous phase, can guarantee that the content of tPSA and the content of fPSA can be detected at the same time, enables a detecting result of tPSA and fPSA in the same sample liquid to be high in accuracy and small in error, greatly shortens reaction time, shortens detection time, improves efficiency, improves detection sensitivity and specificity and brings great convenience to clinical application.

The invention relates to an industrial product defect detection method and an industrial intelligent camera. The industrial product defect detection method comprises the steps: S1, starting an industrial intelligent camera, and an onboard camera chip adjusting controllable light source illumination parameters according to imaging requirements; S2, photographing an industrial product by using the low-distortion lens, and transmitting a photographed product image to the embedded processor through the onboard camera chip; and S3, the embedded processor performing defect identification on the product image by using the deep learning defect detection model, and outputting an inference result to the outside. The industrial intelligent camera carries the deep learning defect detection model, defects with complex structures and complex production environments can be efficiently recognized, meanwhile, the deep learning defect detection model capable of adapting to different workpieces and environments can be loaded and updated through the server, and the industrial intelligent camera is used for collecting images of industrial products. Therefore, instant detection of product defects can berealized.

The invention discloses a method for immediate detection of telomerase activity based on gas pressure change caused by H2O2 (hydrogen peroxide) decomposition catalyzed by a platinum nanoparticle. The method comprises the steps: when active telomerase exists, a telomerase primer modified on the surface of a magnetic bead is bonded to telomerase, thus the telomerase primer is extended along a 3' end of the telomerase primer and a DNA single strand with TTAGGG repeats is formed; the long DNA single strand can hybridize with multiple cDNAs, and by modifying the surface of the platinum nanoparticle with the cDNA, the platinum nanoparticle is fixed to the surface of the magnetic bead; then the platinum nanoparticle is utilized to catalyze H2O2 decomposition to produce oxygen, and the gas pressure of a system changes; then a portable barometer is adopted for detecting a gas pressure value of the system, so that the immediate detection of the telomerase activity can be realized. The detection method disclosed by the invention is simple, rapid and sensitive, and can be used for detecting the expression level of telomerase activity in different tumor cells, and the detection method is of great significance in the diagnosis and treatment of cancers with telomerase as a target point.

The invention discloses a colloidal gold chromatography anti-SSB antibody detection test paper and a preparation method thereof. The colloidal gold chromatography anti-SSB antibody detection test paper comprises a sample pad, a combination pad, a cellulose nitrate coated film and a water absorption pad, and the sample pad, the combination pad, the cellulose nitrate coated film and the water absorption pad are pasted on a base plate sequentially from one side of the base plate to the other side of the base plate, wherein the combination pad is coated with a gold-labeled antibody a and a gold-labeled antibody b; and the cellulose nitrate coated film is provided with a detection line and a quality control line, the detection line is coated with an SSB antigen protein, and the quality control line is coated with a gold-labeled antibody c. By adopting indirect immunoassay to introduce the SSB antigen protein in the invention to carry out process optimization on the combination pad and the sample pad, so high sensitivity, high specificity and high accuracy detection performances of the anti-SSB antibody are realized, and a reference basis is provided for the auxiliary diagnosis of primary biliary cirrhosis.

The invention discloses a colloidal gold chromatography anti-Ro52 antibody detection test paper and a preparation method thereof. The colloidal gold chromatography anti-Ro52 antibody detection test paper comprises a sample pad, a combination pad, a cellulose nitrate coated film and a water absorption pad, and the sample pad, the combination pad, the cellulose nitrate coated film and the water absorption pad are pasted on a base plate sequentially from one side of the base plate to the other side of the base plate, wherein the combination pad is coated with a gold-labeled antibody a and a gold-labeled antibody b; and the cellulose nitrate coated film is provided with a detection line and a quality control line, the detection line is coated with an Ro52 antigen protein, and the quality control line is coated with a gold-labeled antibody c. By adopting indirect immunoassay to introduce the Ro52 antigen protein in the invention to carry out process optimization on the combination pad and the sample pad, so high sensitivity, high specificity and high accuracy detection performances of the anti-Ro52 antibody are realized, and a reference basis is provided for the auxiliary diagnosis of Sjogren's syndrome.

The invention discloses a composite electrode and a preparation method and application thereof, and belongs to the technical field of electrochemical biosensors. A composite electrode includes: a conductive substrate; a polyaniline/graphene composite layer; a polyaniline/graphene composite layer which coats the surface of the conductive substrate; and gold-platinum bimetallic nanoparticles, wherein gold-platinum bimetallic nanoparticles are deposited on the surface of the polyaniline/graphene composite layer. According to the composite electrode provided by the invention, through the synergistic effect of all the parts, the stability of the composite electrode and the detection sensitivity to hormones are improved, and dopamine, 5-hydroxytryptamine and melatonin can be detected at the same time.

The invention provides an immunomagnetic bead-based time-resolved fluorescence immunoassay kit of cardiac myosin binding protein C (cMyBP-C). The kit includes a calibrator, immunomagnetic beads, immunofluorescence microspheres, analysis buffering liquid and wash liquid. The magnetic beads are coupled with antibodies, and the time-resolved fluorescence microspheres are coupled with the antibodies;thus the two parts form immunomagnetic beads-cMyBP-C antigen-immunofluorescence microsphere complexes with cMyBP-C antigens in a sample in a reaction tube after shaking incubation; a time-resolved instrument is used to determine intensity of fluorescence emitted thereby under excitation of ultraviolet light; and comparison of a standard curve is carried out to determine the amount of the cMyBP-C antigens in the sample. The kit of the invention greatly shortens reaction time, and improves efficiency and sensitivity of detection.

The invention discloses a colloidal gold chromatography anti-Sp100 antibody detection test paper and a preparation method thereof. The colloidal gold chromatography anti-Sp100 antibody detection test paper comprises a sample pad, a combination pad, a cellulose nitrate coated film and a water absorption pad, and the sample pad, the combination pad, the cellulose nitrate coated film and the water absorption pad are pasted on a base plate sequentially from one side of the base plate to the other side of the base plate, wherein the combination pad is coated with a gold-labeled antibody a and a gold-labeled antibody b; and the cellulose nitrate coated film is provided with a detection line and a quality control line, the detection line is coated with an Sp100 antigen protein, and the quality control line is coated with a gold-labeled antibody c. By adopting indirect immunoassay to introduce the Sp100 antigen protein in the invention to carry out process optimization on the combination pad and the sample pad, so high sensitivity, high specificity and high accuracy detection performances of the anti-Sp100 antibody are realized, and a reference basis is provided for the auxiliary diagnosis of primary biliary cirrhosis.

The present invention relates to the field of reagent transport, and provides a pipetting needle and pipetting device. The pipetting needle and pipetting device comprises a fixed block, a needle sheath, a needle and an electrode; the fixed block is provided with a fixed block through-hole for putting the needle sheath; fixed block through-hole comprises a first through-hole and a second through-hole; the second through-hole is located on the first through-hole; the needle is vertically fixed on the needle sheath and passed through the first through-hole; the needle sheath is movebly fixed to the second through-hole; the electrode comprises a first needle sheath electrode and a fixed block through-hole electrode; the first needle sheath electrode is arranged on the side of the needle sheath; the fixed block through-hole electrode is arranged on the inner wall of the fixed block, and corresponding to the first needle sheath electrode; and there is gaps between the first needle sheath electrode and the fixed block through-hole electrode, and when collision occurs to the needle, the two electrodes are communicated. According to the pipetting needle and pipetting device disclosed by the present invention, immediate detection of collision occurring to the needle is realized, collision resistance is improved, damage of the pipetting needle and pipetting device is avoided, and a service life of the pipetting needle and pipetting device is prolonged.

The invention relates to the field of a medical appliance, in particular to a chemiluminescence fast diagnosis preincubation device, a preincubation assembly and a method. Through the arrangement of an independent heating preincubation disc and a reagent disc, reagents and magnetic beads are preheated in advance; through the matching of a suction needle assembly and the preincubation device, the instant detection of the sample is realized; the sample detection time can be saved; the sample detection efficiency is effectively improved. During the sample detection, the preheated reagents and the magnetic beads can be directly added into a sample for chemical reaction; the incubation time of the reagents and the magnetic beads is greatly shortened. The method belongs to a fast diagnosis method.

The invention provides an HLA-B*5801 genetype rapid detection kit based on a POCT mode. The kit at least contains fluorogenic quantitative PCR primers and probes (SEQ ID NO:1-4) as well as a cell lysis solution, and can further comprise dNTPs, DNA polymerase, Mg<2+>, a reaction buffer, a standard positive template, a sampling bar, a sample collecting tube and the like, wherein the fluorogenic quantitative PCR primers and the probes are used for detecting the HLA-B*5801 (rs9263726) genetypes. The kit can realize instant detection, DNA purification is not needed, a sample can be directly added into a reagent for carrying out a PCR reaction, the kit is particularly suitable for rapidly and accurately detecting samples (such as shedding buccal cells) with low content of DNA, the detection accuracy rate achieves 99% or above, the detection sensitivity is high, and genomic DNA of which the amount is as low as 0.125ng can be accurately detected; and the whole detection consumes short time, adetection result can be obtained within 1h, an administration basis can be provided for a doctor at first time, and thus the administration risk of patients is reduced.

The invention relates to a biological detection kit, in particular to a time-resolved fluorescence immunoassay kit for detecting a tissue plasminogen activator/plasminogen activator inhibitor 1 compound (t-PAI-C) and application, and belongs to the technical field of immunoassay analysis and nano-biology. The time-resolved fluoroimmunoassay kit for detecting a tissue plasminogen activator/plasminogen activator inhibitor 1 compound (t-PAI-C) comprises a reaction buffer solution, a cleaning solution, an enhancement solution, a t-PAI-C antigen-coated magnetic particle solution, a europium-labeledgoat anti-human IgG antibody solution and a t-PAI-C freeze-dried calibration product which are integrated on a reagent strip. The kit provided by the invention can provide a reaction system close toa homogeneous phase, detect the content of tPAIC, shorten the detection time, improve the efficiency and provide convenience for clinical use.

The invention provides a silicon carbide metal oxide semiconductor field effect transistor and a manufacturing method thereof. A temperature sensor P+ ion implantation region and a temperature sensor N+ ion implantation region are formed in a third P well region through ion implantation; and the temperature sensor P+ ion implantation region and the temperature sensor N+ ion implantation region form a PN junction diode. Real-time detection of an internal temperature of a silicon carbide device is achieved through integrating a PN junction temperature sensor in a P well of a silicon carbide VDMOS; the silicon carbide metal oxide semiconductor field effect transistor can be applied to temperature detection at a high temperature; the influence of the sensor on reverse withstand voltage of the silicon carbide VDMOS is eliminated; the layout overhead is minimized; and the silicon carbide metal oxide semiconductor field effect transistor has good compatibility with an existing VDMOS manufacturing technology.

The invention discloses a kit for detecting keratoprotein 19 fragment and carcino-embryonic antigen contents in a sample based on a luminous quantum dot nanometer microemulsion probe. The kit comprises an immunochromatography detection test strip and the functional quantum dot nanometer microemulsion probe. The preparation method of the functional quantum dot nanometer microemulsion probe comprises the following steps of embedding or wrapping oil soluble quantum dots with emission wavelength between 520-620nm into modified polystyrene nanoparticles to acquire quantum dot nanometer microemulsion, covalently linking the quantum dot nanometer microemulsion with a detection antibody, and performing microemulsion treatment on the surface of the microemulsion to acquire the functional quantum dot nanometer microemulsion probe. The kit provided by the invention is based on novel fluorescence immunochromatography, avoids errors caused by identification by naked eyes, and can rapidly, synchronously, quantitatively and accurately detect a plurality of biological targets.

The invention discloses a colloidal gold chromatography anti-nucleosome antibody detection test paper and a preparation method thereof. The colloidal gold chromatography anti-nucleosome antibody detection test paper comprises a sample pad, a combination pad, a cellulose nitrate coated film and a water absorption pad, and the sample pad, the combination pad, the cellulose nitrate coated film and the water absorption pad are pasted on a base plate sequentially from one side of the base plate to the other side of the base plate, wherein the combination pad is coated with a gold-labeled antibody a and a gold-labeled antibody b; and the cellulose nitrate coated film is provided with a detection line and a quality control line, the detection line is coated with a nucleosome antigen protein, and the quality control line is coated with a gold-labeled antibody c. By adopting indirect immunoassay to introduce the nucleosome antigen protein in the invention to carry out process optimization on the combination pad and the sample pad, so high sensitivity, high specificity and high accuracy detection performances of the anti-nucleosome antibody are realized, and a reference basis is provided for the auxiliary diagnosis of systemic lupus erythematosus.