9results about How to "Realize instant detection" patented technology

The invention discloses a split type micro-fluidic chip and a working method thereof. In the chip, a reagent storage module is connected with a reaction module through a connection module, and the reaction module is slidably arranged in a chute of the connection module; a plurality of solution containing cavities are formed in the reagent storage module, a sealing film is attached to the top of the reaction module, and a plurality of solution overflowing cavities are formed in the reaction module; during work, the reaction module and the reagent storage module abut against each other, and when the solution containing cavity is communicated with the atmosphere, directional flowing and releasing of a solution in the solution containing cavity in the micro-fluidic chip to the solution overflowing cavity are achieved through negative pressure pumping. Sample sampling, cleaning, nucleic acid adsorption, elution and reaction in the micro-fluidic chip can be achieved, and instant detection of pathogenic microorganisms is achieved.

The invention provides an MOG-IgG autoantibody detection kit based on a living cell CBA method, a substrate cell for detecting an MOG-IgG antibody is a living cell strain capable of stably expressing an MOG target antigen, the detection substrate cell GFP-MOG-HEK293 cell strain can stably express the MOG antigen, the living substrate cell can retain the complete spatial conformation of the MOG antigen during detection, and the detection sensitivity is high. The detection sensitivity of the MOG-IgG autoantibody is improved.

The invention discloses lateral flow immunochromatography test paper based on phenylboronic acid directional coupling antibody mediation and a detection method of the lateral flow immunochromatography test paper. According to the lateral flow immunochromatography test paper, the phenylboronic acid directional coupling antibody is adopted, the detection sensitivity of LFIA is improved, specific coupling is conducted on the Fc segment of the antibody, the antigen capture rate is increased, and signal amplification is achieved; meanwhile, the nano-particles are combined, and the detection line is colored by utilizing the color of the nano-particles, so that the portability and the sensitivity of the whole detection are greatly improved; the detection method based on the test paper can realize qualitative analysis on a target object by observing the color depth on the detection line with naked eyes or quantitative analysis on the content of the target object by measuring the RGB value of the detection line, has the advantages of strong specificity on the target object, high sensitivity, convenience in use, simple and rapid detection process and safe and reliable detection result, and has a great application prospect.

The invention relates to the technical field of biological detection, and particularly discloses immunochromatography detection test paper for rapid semi-quantitative detection of urine exosome soluble Fms-like tyrosine kinase receptor 1 and a detection method of the immunochromatography detection test paper. The immunochromatography detection test paper can rapidly detect the content of soluble Fms-like tyrosine kinase-1 in urine exosome; whether a patient suffers from preeclampsia or not can be judged through the test paper without depending on instruments and equipment. The immunochromatography test paper provided by the invention can be used for diagnosing and distinguishing the contents of soluble Fms-like tyrosine kinase-1 in pregnant women, namely 4ng / mL and 10ng / mL, and can be used for accurately judging high-risk groups. According to the application, the detection time is shortened, the detection accuracy is also improved, instant detection, mobile detection and household self-service detection are realized, the content and change of soluble Fms-like tyrosine kinase-1 can be automatically and timely mastered, and the risk of preeclampsia occurrence can be predicted in advance.

The invention relates to the field of cup body thickness testing, in particular to a cup body thickness compression molding process detection device and a detection method.The cup body thickness compression molding process detection device comprises sliding columns, compression columns and a cup which are arranged on a base plate, the sliding columns are arranged on the periphery of the base plate, a compression plate slides on the sliding columns jointly, and a plurality of compression shafts are arranged at the lower end of the compression plate; a pressing ring is arranged at the lower end of the pressing shaft, a pushing assembly is arranged on the base plate, an annular groove is formed in the lower end of the pressing ring, a rotating ring is rotationally arranged in the annular groove, a wiping assembly used for wiping a cup is arranged on the inner diameter of the rotating ring, and a detection unit is further arranged on the inner wall of the rotating ring; the wiping assembly automatically wipes the outer side of the cup body before detection, particle impurities are removed, the phenomenon that the impurities interfere with the detection process is avoided, and the detection unit adopts a contact detection design and is not affected by factors such as ambient light, temperature and dust.

The invention discloses a freeze-drying reagent for citric acid determination. The freeze-drying reagent is prepared from a first freeze-drying reagent ball and a second freeze-drying reagent ball which are obtained by freeze-drying liquid drops of a first citric acid determination reagent and a second citric acid determination reagent, the first citric acid determination reagent comprises citrate lyase, lactic dehydrogenase, malate dehydrogenase, a first buffer solution, an additive, a first cryoprotectant and a first surfactant; the second citric acid determination reagent comprises reduced coenzyme, a second surfactant, a second buffer solution and a second cryoprotectant. The biochemical analysis chip comprises a chip body, the chip body is provided with a colorimetric hole and a diluent groove, the first freeze-drying reagent ball and the second freeze-drying reagent ball are contained in the colorimetric hole, and diluent is contained in the diluent groove. The method for measuring the citric acid comprises the following steps: treating a sample, and adding the treated sample into a heated colorimetric hole to react with a freeze-drying reagent ball; testing the absorbance variation and / or the decline rate of the absorbance value of the solution in the colorimetric hole under the specific wavelength; based on this, a citric acid concentration value is determined.

The application discloses a kind of plate thickness and wedge-shaped edge detection device, with conveying assembly, cleaning assembly, laser detector and warning assembly;Conveying assembly is used to convey gypsum board, and the both sides of conveying assembly are provided with notch;Cleaning assembly is set to the upstream of conveying assembly, for removing water stain dust on the surface of gypsum board;Laser detector is symmetrically set to the both sides of conveying assembly, and the upper end and middle part of laser detector are respectively provided with two laser sensors and controller, and laser sensor is electrically connected to controller;Laser detector side is also provided with warning assembly, and controller can control warning assembly to send alarm when receiving abnormal signal.The application exposes the edge of gypsum board by setting notch on the side of conveying assembly, and sets laser detector at the notch, so that two laser sensors can detect the distance from the upper and lower surfaces of gypsum board when gypsum board passes through laser detector, so as to obtain the edge and thickness of gypsum board, and realize the instant detection of the edge thickness of gypsum board.

The invention relates to the technical field of copper electrolysis, in particular to a rapid colorimetric analysis method for the content of thiourea in copper electrolyte, which comprises the following steps: preparation before detection: preparing a reagent and preparing a sample; preparing a standard color card: taking different volumes of thiourea standard solutions, respectively adding reagents for reaction, inserting starch potassium iodide test paper for color development, and making the standard color card according to different color development conditions; treating a sample: taking a certain sample, diluting to a detection range, and adding a reagent for reaction; and colorimetric analysis of the thiourea content in the sample: inserting the starch potassium iodide test paper into a solution obtained after the reaction of the iodine solution and the thiourea, carrying out colorimetric analysis with a standard color card after color development so as to obtain the content range of the thiourea in the sample to be detected, and analyzing the gray value after photographing the color development test paper so as to further determine the accurate content of the thiourea in the sample. According to the method, the content of the thiourea in the copper electrolyte can be quickly, efficiently, simply and conveniently obtained on site without an instrument, and the content of the thiourea in the copper electrolyte can be judged in time.

This invention relates to the field of biodetection technology, providing an enzyme cascade reaction system for the detection of nucleic acids and protein molecules, comprising BER endonuclease and Argonaute endonuclease; wherein the BER endonuclease is one or more selected from APE1, FEN1, Fpg, UDG, and T7endonuclease, and the Argonaute endonuclease is one or more selected from pfAgo, CpAgo, CbAgo, TtAgo, RasAgo, MjAgo, and NgAgo. This invention also provides a method for detecting nucleic acids and protein molecules using the above system, and the application of the above system in the preparation of target nucleic acid and target protein molecule products from detection samples. This invention utilizes the synergistic enzyme activity of APE1 and PfAgo in the enzyme cascade reaction system to achieve real-time detection of nucleic acid and protein biomarkers without pre-amplification within 45 minutes.