83results about How to "Realize instant detection" patented technology

The invention relates to a primer composite for detecting respiratory pathogens. The primer composite comprises at least one group of a mycoplasma pneumoniae group, a chlamydia pneumoniae primer group, an influenza A / B virus primer group, a parainfluenza virus primer group, an adenovirus primer group and a respiratory syncytial virus primer group. The invention further relates to a kit comprising the primer composite. The kit further comprises a micro-fluidic chip wrapping primers, and a macroscopic indicator. The invention further relates to a detection method adopting the primer composite. The detection method comprises the steps of primer composite coating, to-be-detected sample nucleic acid extraction, LAMP reaction and visual result interpretation. The kit and the method are applied to the micro-fluidic chip for visual judgment, instant detection of the seven respiratory pathogens is rapidly and accurately realized, and a result is judged by a naked eye by color change, so that the kit and the method are simpler, more convenient and quicker in practical applications, are easy to operate, and are suitable for site operation.

The invention discloses a magnetic bead time resolution fluorescence immunoassay quantitative determination CK-MB kit. The CK-MB kit comprises an immunomagnetic bead coating a CK-MB monoclonal antibody, a CK-MB standardized product solution, a europium-marked CK-MB monoclonal antibody solution, washing liquid and enhancement liquid. The immunomagnetic bead coating the CK-MB monoclonal antibody isa covalent conjugate of a superpara magnetic bead modified by a functional group and with the diameter being 1-3 microns and the CK-MB monoclonal antibody. The kit has the high sensibility, the sensibility of CK-MB is 1ng / mL, and a blood serum (plasma) does not need to be diluted; the determination time is short, and a report can be resulted within 30 minutes; the demanding amount of the sample isless, and only 50 microliters are needed for one-time sample loading; and the kit is equipped with a full-automatic time resolution immune analysis meter, operation is easy, no artificial error exists, and labor is saved. The kit reasonably utilizes the space of a reagent strip, the structure of the reagent strip is more compact, the reagent strip can be transported more easily, and used conveniently, the operation is simple, and the stability is good.

The invention relates to a double-tagging time resolution fluoroimmunoassay reagent kit based on a PG magnetic particle and a preparing method and detecting method thereof and belongs to the technical field of immune detection analyzing techniques and nanometer biological techniques. The reagent kit comprises a reaction buffer solution, a concentrated cleaning solution, an enhancing solution, a magnetic particle solution coated with PGI monoclonal antibody, a magnetic particle solution coated with PGI monoclonal antibody, a PG calibrator solution, an europium-labeled PGII monoclonal antibody solution and a samarium-labeled PGI monoclonal antibody solution which are independently packaged. The reagent kit can provide a double-tagging reaction system close to a homogeneous phase, can guarantee that the content of PGI and the content of PGII can be detected at the same time, enables a detecting result of PGI and PGII in the same sample liquid to be high in accuracy and small in error, greatly shortens reaction time, shortens detection time, improves efficiency, improves detection sensitivity and specificity and brings great convenience to clinical application.

The invention discloses a colloidal gold chromatography anti-Jo-1 antibody detection test paper and a preparation method thereof. The colloidal gold chromatography anti-Jo-1 antibody detection test paper comprises a sample pad, a combination pad, a cellulose nitrate coated film and a water absorption pad, and the sample pad, the combination pad, the cellulose nitrate coated film and the water absorption pad are pasted on a base plate sequentially from one side of the base plate to the other side of the base plate, wherein the combination pad is coated with a gold-labeled antibody a and a gold-labeled antibody b; and the cellulose nitrate coated film is provided with a detection line and a quality control line, the detection line is coated with a Jo-1 antigen protein, and the quality control line is coated with a gold-labeled antibody c. By adopting indirect immunoassay to introduce the Jo-1 antigen protein in the invention to carry out process optimization on the combination pad and the sample pad, so high sensitivity, high specificity and high accuracy detection performances of the anti-Jo-1 antibody are realized, and a reference basis is provided for the auxiliary diagnosis of dermatomyositis / polymyositis.

The invention provides a time resolution fluorescence immunoassay method based on magnetic separation; the method mainly comprises the following steps of firstly, coupling magnetic beads with an antibody to form immunomagnetic beads; meanwhile, enabling time resolution fluorescent microsphere to be coupled with antibody to form immunofluorescent microspheres; then enabling the immunomagnetic beadsand the immunofluorescent microspheres and the antigen in a sample to be oscillated and incubated in a reaction tube to form an immunomagnetic bead-antigen-immunofluorescent microsphere compound; andfinally, testing the fluorescence intensity, emitted by excitation of the compound at 360 nm excitation, by a time resolution instrument, wherein a standard curve is used as reference for determiningthe amount of the antigen in the sample. According to the analysis method, the reaction time is greatly shortened, and the detection efficiency and sensitivity are improved.

The invention relates to a double-tagging time resolution fluoroimmunoassay reagent kit based on a PSA magnetic particle and a preparing method and detecting method thereof and belongs to the technical field of immune detection analyzing techniques and nanometer biological techniques. The reagent kit comprises a reaction buffer solution, a concentrated cleaning solution, an enhancing solution, a PSA monoclonal antibody coated magnetic particle solution, a PSA calibrator solution, an europium-labeled fPSA monoclonal antibody solution and a samarium-labeled tPSA monoclonal antibody solution which are independently packaged. The reagent kit can provide a double-tagging reaction system close to a homogeneous phase, can guarantee that the content of tPSA and the content of fPSA can be detected at the same time, enables a detecting result of tPSA and fPSA in the same sample liquid to be high in accuracy and small in error, greatly shortens reaction time, shortens detection time, improves efficiency, improves detection sensitivity and specificity and brings great convenience to clinical application.

The invention discloses an electrochemical sensor, a preparation method and application thereof in quick detection of aflatoxin B1 (AFB1). The electrochemical sensor comprises a three-electrode system and a detection cell, wherein the three-electrode system comprises a working electrode, an Ag / AgCl reference electrode and a platinum wire counter electrode, and the working electrode is a modified gold electrode; the detection cell is filled with a base solution formed by an electroactive indicator and a buffer solution; the electrodes are modified by utilizing DAN to perform electrochemical detection on AFB1, expensive monoclonal AFB1 antibody is not needed, the problems of complicated operation, high cost, high sample amount, long time consumption and the like of general chromatography and immunological detection methods can be solved, and instant detection of AFB1 is realized. The electrochemical sensor is convenient and quick, has the advantages of high detection sensitivity, 10 ng / mL low detection limit, small required sample amount, extremely low detection cost, small interference and the like, and can be used for quickly, easily, conveniently and accurately quantitatively detecting AFB1.

The invention relates to an industrial product defect detection method and an industrial intelligent camera. The industrial product defect detection method comprises the steps: S1, starting an industrial intelligent camera, and an onboard camera chip adjusting controllable light source illumination parameters according to imaging requirements; S2, photographing an industrial product by using the low-distortion lens, and transmitting a photographed product image to the embedded processor through the onboard camera chip; and S3, the embedded processor performing defect identification on the product image by using the deep learning defect detection model, and outputting an inference result to the outside. The industrial intelligent camera carries the deep learning defect detection model, defects with complex structures and complex production environments can be efficiently recognized, meanwhile, the deep learning defect detection model capable of adapting to different workpieces and environments can be loaded and updated through the server, and the industrial intelligent camera is used for collecting images of industrial products. Therefore, instant detection of product defects can berealized.

The invention discloses a method for immediate detection of telomerase activity based on gas pressure change caused by H2O2 (hydrogen peroxide) decomposition catalyzed by a platinum nanoparticle. The method comprises the steps: when active telomerase exists, a telomerase primer modified on the surface of a magnetic bead is bonded to telomerase, thus the telomerase primer is extended along a 3' end of the telomerase primer and a DNA single strand with TTAGGG repeats is formed; the long DNA single strand can hybridize with multiple cDNAs, and by modifying the surface of the platinum nanoparticle with the cDNA, the platinum nanoparticle is fixed to the surface of the magnetic bead; then the platinum nanoparticle is utilized to catalyze H2O2 decomposition to produce oxygen, and the gas pressure of a system changes; then a portable barometer is adopted for detecting a gas pressure value of the system, so that the immediate detection of the telomerase activity can be realized. The detection method disclosed by the invention is simple, rapid and sensitive, and can be used for detecting the expression level of telomerase activity in different tumor cells, and the detection method is of great significance in the diagnosis and treatment of cancers with telomerase as a target point.

The invention discloses a colloidal gold chromatography anti-SSB antibody detection test paper and a preparation method thereof. The colloidal gold chromatography anti-SSB antibody detection test paper comprises a sample pad, a combination pad, a cellulose nitrate coated film and a water absorption pad, and the sample pad, the combination pad, the cellulose nitrate coated film and the water absorption pad are pasted on a base plate sequentially from one side of the base plate to the other side of the base plate, wherein the combination pad is coated with a gold-labeled antibody a and a gold-labeled antibody b; and the cellulose nitrate coated film is provided with a detection line and a quality control line, the detection line is coated with an SSB antigen protein, and the quality control line is coated with a gold-labeled antibody c. By adopting indirect immunoassay to introduce the SSB antigen protein in the invention to carry out process optimization on the combination pad and the sample pad, so high sensitivity, high specificity and high accuracy detection performances of the anti-SSB antibody are realized, and a reference basis is provided for the auxiliary diagnosis of primary biliary cirrhosis.

The invention provides a rapid detection kit for CYP2C19*2 or *3 genotype based on a POCT mode. The kit contains at least a fluorescent quantitative PCR primer and probe for detecting a CYP2C19*2 (rs4244285, SEQ ID NO: 1-4) or CYP2C19*3 (rs4986893, SEQ ID NO: 5-8) genotype of and a cell lysate. In addition, the kit may comprise dNTPs, DNA polymerase, Mg<2+>, a reaction buffer, a standard positivetemplate, a sampling rod, a sample collection tube and the like. The kit can realize real-time detection without DNA purification. A sample can be directly added into a reagent for a PCR reaction, thekit is especially suitable for rapid accurate detection of samples with low DNA content (such as oral exfoliated cells), and the detection accuracy is 99% or more. The detection sensitivity is high,and the detection limit of genomic DNA as low as 0.125 ng can be accurately detected. The whole detection takes a short time, and a detection result can be obtained within one hour and provides a medicine use basis for a doctor at the first time, thereby reducing the risk of medicine administration.

The invention relates to the field of microbiological detection, in particular to a microbiological detection system and method. The microbiological detection system realizes real-time detection of microbial metabolism and reproduction states under cooperation between a control device and a light-emitting source device, a fluorescence receiving device and a microbial culture medium placement device. The microbiological detection system is high in reliability and good in accuracy. The microbiological detection method comprises the following steps: at first, a light-emitting source device emits ultraviolet light, and a fluorescent effect is generated between the ultraviolet light and a microbial culture medium; then the fluorescence receiving device receives fluorescence generated by the fluorescent effect, and transmits the concentration data of a microbial metabolite corresponding to the fluorescence to the control device; finally, the control device receives the concentration data, transmitted by the fluorescence receiving device, of the microbial metabolite and performs analysis to obtain the concentration of the microbial metabolite. The microbiological detection method is high in detection precision and efficiency.

The invention discloses a colloidal gold chromatography anti-Ro52 antibody detection test paper and a preparation method thereof. The colloidal gold chromatography anti-Ro52 antibody detection test paper comprises a sample pad, a combination pad, a cellulose nitrate coated film and a water absorption pad, and the sample pad, the combination pad, the cellulose nitrate coated film and the water absorption pad are pasted on a base plate sequentially from one side of the base plate to the other side of the base plate, wherein the combination pad is coated with a gold-labeled antibody a and a gold-labeled antibody b; and the cellulose nitrate coated film is provided with a detection line and a quality control line, the detection line is coated with an Ro52 antigen protein, and the quality control line is coated with a gold-labeled antibody c. By adopting indirect immunoassay to introduce the Ro52 antigen protein in the invention to carry out process optimization on the combination pad and the sample pad, so high sensitivity, high specificity and high accuracy detection performances of the anti-Ro52 antibody are realized, and a reference basis is provided for the auxiliary diagnosis of Sjogren's syndrome.

The invention discloses a composite electrode and a preparation method and application thereof, and belongs to the technical field of electrochemical biosensors. A composite electrode includes: a conductive substrate; a polyaniline / graphene composite layer; a polyaniline / graphene composite layer which coats the surface of the conductive substrate; and gold-platinum bimetallic nanoparticles, wherein gold-platinum bimetallic nanoparticles are deposited on the surface of the polyaniline / graphene composite layer. According to the composite electrode provided by the invention, through the synergistic effect of all the parts, the stability of the composite electrode and the detection sensitivity to hormones are improved, and dopamine, 5-hydroxytryptamine and melatonin can be detected at the same time.

The invention provides an immunomagnetic bead-based time-resolved fluorescence immunoassay kit of cardiac myosin binding protein C (cMyBP-C). The kit includes a calibrator, immunomagnetic beads, immunofluorescence microspheres, analysis buffering liquid and wash liquid. The magnetic beads are coupled with antibodies, and the time-resolved fluorescence microspheres are coupled with the antibodies;thus the two parts form immunomagnetic beads-cMyBP-C antigen-immunofluorescence microsphere complexes with cMyBP-C antigens in a sample in a reaction tube after shaking incubation; a time-resolved instrument is used to determine intensity of fluorescence emitted thereby under excitation of ultraviolet light; and comparison of a standard curve is carried out to determine the amount of the cMyBP-C antigens in the sample. The kit of the invention greatly shortens reaction time, and improves efficiency and sensitivity of detection.

The present invention relates to the field of reagent transport, and provides a pipetting needle and pipetting device. The pipetting needle and pipetting device comprises a fixed block, a needle sheath, a needle and an electrode; the fixed block is provided with a fixed block through-hole for putting the needle sheath; fixed block through-hole comprises a first through-hole and a second through-hole; the second through-hole is located on the first through-hole; the needle is vertically fixed on the needle sheath and passed through the first through-hole; the needle sheath is movebly fixed to the second through-hole; the electrode comprises a first needle sheath electrode and a fixed block through-hole electrode; the first needle sheath electrode is arranged on the side of the needle sheath; the fixed block through-hole electrode is arranged on the inner wall of the fixed block, and corresponding to the first needle sheath electrode; and there is gaps between the first needle sheath electrode and the fixed block through-hole electrode, and when collision occurs to the needle, the two electrodes are communicated. According to the pipetting needle and pipetting device disclosed by the present invention, immediate detection of collision occurring to the needle is realized, collision resistance is improved, damage of the pipetting needle and pipetting device is avoided, and a service life of the pipetting needle and pipetting device is prolonged.

The invention provides a PAR lamp fixed focus detection device and a fixed focus detection method thereof. The device comprises a test power supply, an illumination meter, a test tube, a tripod, a frame, a vacuum pipeline, a vacuum test chamber, a vacuum pump and a vacuum meter. The vacuum pump is arranged and fixed in the frame. The vacuum test chamber is connected with the vacuum pump through the vacuum pipeline. The vacuum meter is connected with the vacuum pipeline. The tripod is located on the front end of the vacuum test chamber. The test tube is fixedly arranged at the top of the tripod. A photosensitive probe body is located in the test tube. The test power supply and the illumination meter are placed on the frame. The illumination meter is connected with the photosensitive probe. The test power supply is connected with the vacuum test chamber. According to the invention, fixed focus quality real-time detection is carried out; a detected unqualified fixed focus product can be reworked; and the problem that the unqualified fixed focus product cannot be reworked but scrapped due to the fact that a follow-up process is completed in a traditional process is effectively avoided.

The invention discloses an adjustable glass curtain wall support which is formed by combination of glass fixing blocks, a connecting base and connecting arms, wherein the glass fixing blocks are used for fixing corners of mutually combined glass panes which are then fixedly connected through the connecting base. According to the adjustable glass curtain wall support, piezoelectric ceramic bodies are arranged inside the connecting arms, and the acting directions of the piezoelectric ceramic bodies are parallel to the lines connecting the connecting base with the glass fixing blocks; when glass expands under heat or contracts under cold, the connecting arms can stretch or retract slightly by changing the electric field of the piezoelectric ceramic bodies, and then deformation generated when the glass expands or contracts is corrected, and breakage of the glass due to excessive deformation is prevented. The adjustable glass curtain wall support is high in social benefit and economic benefit.

The invention relates to the field of a medical appliance, in particular to a chemiluminescence fast diagnosis preincubation device, a preincubation assembly and a method. Through the arrangement of an independent heating preincubation disc and a reagent disc, reagents and magnetic beads are preheated in advance; through the matching of a suction needle assembly and the preincubation device, the instant detection of the sample is realized; the sample detection time can be saved; the sample detection efficiency is effectively improved. During the sample detection, the preheated reagents and the magnetic beads can be directly added into a sample for chemical reaction; the incubation time of the reagents and the magnetic beads is greatly shortened. The method belongs to a fast diagnosis method.

The invention belongs to the technical field of instant detection of polymer microfluidic chips, and particularly relates to a POCT microfluidic chip with integrated self-aligning lenses and a preparation method of the POCT microfluidic chip. The microfluidic chip comprises a cover sheet layer, a microchannel and lens layer and a bottom sheet layer; the microchannel and lens layer is provided witha microchannel and the integrated self-aligning lenses on both sides of the microchannel; the integrated self-aligning lenses are of a specific lens arc-surface structure, the radius of curvature andthe focal length of the lenses are determined according to the geometric optical theory, and it is ensured that the focuses of the lens are located in a detection area of the microchannel; the thickness of the microchannel and lens layer of the microfluidic chip is 50-5,000 microns; the shapes, sizes and relative positions of the microchannel and the lens arc structures are designed in laser drawing software, processing and forming are conducted at a time through laser machining, the self-aligning property is achieved, and the complicated optical aligning process is avoided. The lenses are integrated on the microfluidic chip, the size and cost of detection equipment are reduced, and important significance is provided for the achievement of real instant detection.

The invention discloses a TRFIA (time-resolved fluorescence immunoassay) method based on magnetic particles. The method comprises the following steps: coating a magnetic bead with a specific antigen or antibody to form an immune magnetic bead; labelling a chelate formed by polyamino polycarboxy and rare earth ions with the specific antigen or antibody to form a fluorescent label; adding a sample for immune reaction to enable the immune magnetic bead, the tested sample and the fluorescent label to form a complex, adding a fluorescent enhancer after cleaning, enabling fluorescence value to be enhanced a million times with a dissociation-enhancement technology, and finally performing detection to obtain information such as concentration of the tested sample. One or more samples can be simultaneously detected, and multiple indexes can be detected in one experiment, so that operation is simple and quick. Sensitivity, repeatability and flexibility of detection are far better than those of traditional microporous plate TRFIA and chromatography methods.

The invention relates to a biological detection kit, in particular to a time-resolved fluorescence immunoassay kit for detecting a tissue plasminogen activator / plasminogen activator inhibitor 1 compound (t-PAI-C) and application, and belongs to the technical field of immunoassay analysis and nano-biology. The time-resolved fluoroimmunoassay kit for detecting a tissue plasminogen activator / plasminogen activator inhibitor 1 compound (t-PAI-C) comprises a reaction buffer solution, a cleaning solution, an enhancement solution, a t-PAI-C antigen-coated magnetic particle solution, a europium-labeledgoat anti-human IgG antibody solution and a t-PAI-C freeze-dried calibration product which are integrated on a reagent strip. The kit provided by the invention can provide a reaction system close toa homogeneous phase, detect the content of tPAIC, shorten the detection time, improve the efficiency and provide convenience for clinical use.

The invention provides a silicon carbide metal oxide semiconductor field effect transistor and a manufacturing method thereof. A temperature sensor P+ ion implantation region and a temperature sensor N+ ion implantation region are formed in a third P well region through ion implantation; and the temperature sensor P+ ion implantation region and the temperature sensor N+ ion implantation region form a PN junction diode. Real-time detection of an internal temperature of a silicon carbide device is achieved through integrating a PN junction temperature sensor in a P well of a silicon carbide VDMOS; the silicon carbide metal oxide semiconductor field effect transistor can be applied to temperature detection at a high temperature; the influence of the sensor on reverse withstand voltage of the silicon carbide VDMOS is eliminated; the layout overhead is minimized; and the silicon carbide metal oxide semiconductor field effect transistor has good compatibility with an existing VDMOS manufacturing technology.

The invention belongs to the technical field of H2S detection, relates to preparation of a fluorescent probe for the H2S detection, a fluorescent test strip for the H2S detection as well as a preparation method and a using method thereof, and aims to solve the technical problems that by the existing conventional method for detecting hydrogen sulfide in a laboratory, the cost is relatively high andthe hydrogen sulfide cannot be detected in real time, the existing hydrogen sulfide detecting fluorescent probe has no identification specificity and is relatively poor in anti-interference ability,and at present, fluorescent test strips which can be applied to detection of the hydrogen sulfide are fewer and the production process is complicated and cumbersome. The fluorescent probe for detecting hydrogen sulfide provided by the invention has a structural formula as shown in the specification. The fluorescent probe is obtained by performing a reaction on 2-(1-phenyl-1H-phenanthro[9,10-d]imidazol-2-)phenol and 2,4-dinitrofluorobenzene under an alkali condition. The fluorescent test strip is prepared in a system comprising methanol (MeOH) and N-2-hydroxyethylpiperazine-N'-2-ethanesulfonicacid (HEPES) (pH of 7.0) according to a volume ratio of 2:8. When the fluorescent test strip is used for detecting a to-be-detected sample, if the test strip is in yellowish green under irradiation ofan ultraviolet lamp, a to-be-detected solution A contains H2S. The fluorescent test strip for detecting hydrogen sulfide can be applied to the field of hydrogen sulfide detection.

The invention discloses a sensing device capable of instantly analyzing the content of tetracycline in liquid. The system is composed of a smart phone, a grating, an optical fiber coupler, a convex lens and a titanium dioxide nanotube structure. The device needs to be exposed to natural light. Firstly, liquid to be detected is dripped on the titanium dioxide nanotube, and the titanium dioxide nanotube reacts with tetracycline in the liquid under the action of ultraviolet light focused by the convex lens; then the flashlight of the mobile phone is used as a light source to enter the end face ofthe optical fiber, irradiates the reacted liquid and is received by the camera of the mobile phone after being reflected; and finally, the captured image is processed by using mobile phone software,and the tetracycline content is detected according to the obtained spectral data. The sensitivity, the detection range and the like of the device can be adjusted by controlling the surface appearanceof the titanium dioxide nanotube. The device has the advantages of integration, intelligence, low cost and mass production, and has important application value in the fields of environmental protection and food safety.

The invention discloses a kit for detecting keratoprotein 19 fragment and carcino-embryonic antigen contents in a sample based on a luminous quantum dot nanometer microemulsion probe. The kit comprises an immunochromatography detection test strip and the functional quantum dot nanometer microemulsion probe. The preparation method of the functional quantum dot nanometer microemulsion probe comprises the following steps of embedding or wrapping oil soluble quantum dots with emission wavelength between 520-620nm into modified polystyrene nanoparticles to acquire quantum dot nanometer microemulsion, covalently linking the quantum dot nanometer microemulsion with a detection antibody, and performing microemulsion treatment on the surface of the microemulsion to acquire the functional quantum dot nanometer microemulsion probe. The kit provided by the invention is based on novel fluorescence immunochromatography, avoids errors caused by identification by naked eyes, and can rapidly, synchronously, quantitatively and accurately detect a plurality of biological targets.

The invention discloses a colloidal gold chromatography anti-nucleosome antibody detection test paper and a preparation method thereof. The colloidal gold chromatography anti-nucleosome antibody detection test paper comprises a sample pad, a combination pad, a cellulose nitrate coated film and a water absorption pad, and the sample pad, the combination pad, the cellulose nitrate coated film and the water absorption pad are pasted on a base plate sequentially from one side of the base plate to the other side of the base plate, wherein the combination pad is coated with a gold-labeled antibody a and a gold-labeled antibody b; and the cellulose nitrate coated film is provided with a detection line and a quality control line, the detection line is coated with a nucleosome antigen protein, and the quality control line is coated with a gold-labeled antibody c. By adopting indirect immunoassay to introduce the nucleosome antigen protein in the invention to carry out process optimization on the combination pad and the sample pad, so high sensitivity, high specificity and high accuracy detection performances of the anti-nucleosome antibody are realized, and a reference basis is provided for the auxiliary diagnosis of systemic lupus erythematosus.

The invention discloses a CEA TRFIA (time-resolved fluoroimmunoassay) kit based on IMB (immunomagnetic beads). The kit includes a calibrator, immunomagnetic beads connected with CEA antibodies, europium labeled CEA antibodies, an assay buffer solution, a cleaning solution and an enhanced solution. Through double antibody sandwich immunoreaction, IMB-CEA-europium labeled-CEA monoclonal antibody complexes are formed, IMB which adsorb CEA and supernate are separated through magnetic seperation and washed, the enhanced solution is added, and the value is measured through a time-resolved instrument. Besides the TRFIA technology, the invention further has the advantages as follows: through IMB enrichment and full diffusion of IMB in the solution, the combination superficial area is enlarged, the reaction time is greatly shortened, and the detection sensitivity is improved. IMB and antibodies are directionally connected through chemical groups, so that the consumption of paired antibodies is greatly reduced and the detection precision is improved. The technology realizes automation easily, and overcomes the problem that the traditional micropore plate type TRFIA technology can only carry out detection after samples are accumulated to a certain number, thereby realizing real-time sample detection.