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Method for rapidly preparing nano-silver bacteriostatic agent through ligustrum quihoui fruit extracting solution

A technology of silver bacteriostatic agent and extract solution, which is applied in the field of nanobiology, can solve the problems of unsatisfactory particle size uniformity, poor reproducibility of nano silver, long reaction time of nano silver, etc., achieve significant antibacterial effect, and benefit The effect of promotion and product quality stability

Active Publication Date: 2018-02-09
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual production, it is found that the reaction time of many plant materials to prepare nano-silver is long, and the reproducibility and stability of nano-silver synthesized by different batches of plant materials are not good, and the uniformity of particle size is not ideal.

Method used

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  • Method for rapidly preparing nano-silver bacteriostatic agent through ligustrum quihoui fruit extracting solution
  • Method for rapidly preparing nano-silver bacteriostatic agent through ligustrum quihoui fruit extracting solution
  • Method for rapidly preparing nano-silver bacteriostatic agent through ligustrum quihoui fruit extracting solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Preparation of Nano-silver from Ligustrum lucidum fruit extract

[0021] a, the preparation of Ligustrum lucidum fruit extract

[0022] Ripe privet privet fruits were collected, washed with tap water, rinsed with ultrapure water three times, dried at 60°C to constant weight, and stored for later use.

[0023] Grind the dried privet privet fruit into powder, weigh 1 g of the powder, add 10 mL of ultrapure water, and heat to reflux at 90 ° C for 30 min. After natural cooling, filter under reduced pressure, centrifuge at 12,000 rpm for 10 min, collect the supernatant to obtain Ligustrum lucidum extract, store it at 4°C, and keep it for later use.

[0024] b. Biosynthesis of silver nanoparticles

[0025] Add 1mM AgNO to 1mL Ligustrum microphylla fruit extract 3 19 mL of the solution was stirred evenly, and treated with 100W ultrasonic wave, light, and 100W ultrasonic wave plus light for 30 minutes respectively.

[0026] c. Visible-ultraviolet characterization ...

Embodiment 2

[0028] Example 2 Preparation of nano-silver from privet privet leaf extract

[0029] a, preparation of privet privet leaf extract

[0030] The leaves of Ligustrum lobata were collected, washed with tap water, rinsed with ultrapure water three times, dried at 60°C to constant weight, and stored for later use.

[0031] Weigh 2 g of privet privet fruit powder, add 10 mL of ultrapure water, and heat to reflux at 50 ° C for 2 h. After natural cooling, filter under reduced pressure, centrifuge at 12,000 rpm for 10 min, collect the supernatant to obtain Ligustrum lucidum extract, store it at 4°C, and keep it for later use.

[0032] b. Biosynthesis of silver nanoparticles

[0033] Add 5mM AgNO to 2mL of Ligustrum lucidum fruit extract 3 The solution was 19mL, and after stirring evenly, it was treated with 100W ultrasonic wave and light for 1min, 3min, 5min, 7min, 9min, and 12min respectively, and then samples were taken.

[0034] c. Visible-ultraviolet characterization of silver n...

Embodiment 3

[0036] Example 3 Preparation of nano-silver from extracts from different tissues of Ligustrum lucidum

[0037] a, preparation of privet privet extract

[0038] The leaves, flowers, and fruits of Ligustrum lobata were collected separately, washed with tap water, rinsed with ultrapure water three times, dried at 60°C to constant weight, and stored for later use.

[0039] Weigh the above three kinds of Ligustrum lobata tissues and grind them into powder, take 1g, add 10mL of ultrapure water, and heat to reflux at 90°C for 1h. After natural cooling, filter under reduced pressure, centrifuge at 12,000 rpm for 10 min, collect the supernatant to obtain Ligustrum lucidum extract, store it at 4°C, and keep it for later use.

[0040] b. Biosynthesis of silver nanoparticles

[0041] Take 3 mL of the above-mentioned three kinds of privet privet extracts, add 1 mM AgNO 3 19mL of the solution was stirred evenly, and treated with 100W ultrasonic wave and light for 10min respectively.

[...

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Abstract

The invention discloses a method for rapidly preparing a nano-silver bacteriostatic agent through a ligustrum quihoui fruit extracting solution, and belongs to the technical field of nano-biology. Themethod comprises the following steps that the mature ligustrum quihoui fruit is collected, the ligustrum quihoui fruit is dried to the constant weight after being thoroughly cleaned, and the ligustrum quihoui fruit is ground into powder; the ligustrum quihoui fruit powder is taken, ultra pure water is added, heating and refluxing are conducted, after natural cooling, pressure-reduction suction filtration is conducted, centrifuging is conducted, and finally the ligustrum quihoui fruit extracting solution is obtained; the ligustrum quihoui fruit extracting solution is taken, the AgNO3 solutionis added, diluting to graduation is performed by using the ultra pure water, and the constant-volume solution is arranged in an ultrasound instrument to be subjected to ultrasound treatment; and meanwhile, sunlight irradiation is conducted, and the nano-silver bacteriostatic agent is obtained. According to the method, the ligustrum quihoui fruit extractive is utilized for biologically synthesizingnano-silver, the light-catalyzed reaction with the assistance of ultrasonic waves is adopted, the synthesizing speed is obviously increased, and synthesizing can be finished within 1-15 min. The method is environment-friendly, materials are easy to obtain, the products have high uniformity and dispersibility, the product quality is stable, an obvious antibacterial effect is achieved, the antimicrobial spectrum is wide, and popularization is facilitated.

Description

technical field [0001] The invention relates to the field of nano-biotechnology, in particular to a method for rapidly preparing a nano-silver bacteriostatic agent by using the extract of privet privet fruit, which can be popularized and applied in the fields of agriculture and aquatic products. Background technique [0002] In recent years, with the extensive use of bacteriostatic agents in agricultural production, it has caused increased microbial resistance, serious drug residues, and increased environmental pollution. Therefore, it is very important to research and develop new antibacterial agents that are safe, environmentally friendly and not prone to drug resistance. With the continuous development of nanotechnology, nano-metal bacteriostatic agents have received widespread attention. This kind of metal particles with a particle size between 1 and 100nm has a strong inhibitory and killing effect on a variety of pathogenic microorganisms, and has the advantages of lar...

Claims

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Application Information

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IPC IPC(8): B22F9/24B82Y40/00A01N59/16A01P1/00
CPCA01N59/16B22F9/24B82Y40/00
Inventor 王磊宿红艳坚天才梁徐健戴昕彤
Owner LUDONG UNIVERSITY
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