Method for rapid preparation of nano-silver bacteriostatic agent using privet privet fruit extract

Abstract

The invention discloses a method for rapidly preparing a nano-silver bacteriostatic agent through a ligustrum quihoui fruit extracting solution, and belongs to the technical field of nano-biology. Themethod comprises the following steps that the mature ligustrum quihoui fruit is collected, the ligustrum quihoui fruit is dried to the constant weight after being thoroughly cleaned, and the ligustrum quihoui fruit is ground into powder; the ligustrum quihoui fruit powder is taken, ultra pure water is added, heating and refluxing are conducted, after natural cooling, pressure-reduction suction filtration is conducted, centrifuging is conducted, and finally the ligustrum quihoui fruit extracting solution is obtained; the ligustrum quihoui fruit extracting solution is taken, the AgNO3 solutionis added, diluting to graduation is performed by using the ultra pure water, and the constant-volume solution is arranged in an ultrasound instrument to be subjected to ultrasound treatment; and meanwhile, sunlight irradiation is conducted, and the nano-silver bacteriostatic agent is obtained. According to the method, the ligustrum quihoui fruit extractive is utilized for biologically synthesizingnano-silver, the light-catalyzed reaction with the assistance of ultrasonic waves is adopted, the synthesizing speed is obviously increased, and synthesizing can be finished within 1-15 min. The method is environment-friendly, materials are easy to obtain, the products have high uniformity and dispersibility, the product quality is stable, an obvious antibacterial effect is achieved, the antimicrobial spectrum is wide, and popularization is facilitated.

Description

Technical field [0001] The invention relates to the field of nano-biotechnology, in particular to a method for quickly preparing a nano-silver bacteriostatic agent using an extract of Ligustrum lucidum fruit, which can be popularized and applied in the fields of agriculture and aquaculture. Background technique [0002] In recent years, with the large-scale application of antibacterial agents in agricultural production, it has caused harms such as increased microbial resistance, serious drug residues, and increased environmental pollution. Therefore, it is very important to research and develop new bacteriostatic agents that are safe, environmentally friendly, and resistant to drug resistance. With the continuous development of nanotechnology, nano-metal antibacterial agents have received widespread attention. This kind of metal particles with a particle size between 1-100nm has a strong inhibitory and killing effect on a variety of pathogenic microorganisms, and has the advanta...

AI Technical Summary

Problems solved by technology

However, in actual production, it is found that the reaction time of many plant materials to prepare nano-silver is long, and the reproducibility and stability of nano-silver synthesized by different batches of plant materials are not good, and the uniformity of particle size is not ideal.

Images