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Enzyme electrode, enzyme biosensor as well as preparation methods and application thereof

An enzyme electrode and electrode technology, which is applied in the field of biosensors, can solve the problems of no biosensors, etc., and achieve the effect of enhancing electron transfer rate, reducing pollution, and promoting electron transfer

Active Publication Date: 2014-07-30
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no biosensors made of eggshell membrane materials and nanoparticles at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A method for preparing an enzyme electrode and an enzyme biosensor based on eggshell membrane modification, comprising the steps of:

[0049] (1) The glassy carbon electrode (GCE) is made of 1.0μm, 0.3μm and 0.05μm Al 2 o 3 The slurry is polished to a mirror surface on a suede polishing machine. After each polishing, the surface dirt is washed away with water, and then ultrasonically cleaned with ethanol and distilled water for 3 minutes respectively.

[0050] (2) The eggshell membrane is selected from the inner membrane of the eggshell, the inner membrane of the eggshell is peeled off and cleaned with water, cut into small pieces and placed on the surface of the polished glassy carbon electrode, and placed in an oven at room temperature, starting from room temperature Raise the temperature by 5°C every 5min, then raise the temperature to 80°C, and dry for 4h.

[0051] (3) Put the dried film-loaded glassy carbon electrode into a quartz glass tube, put the quartz glass...

Embodiment 2

[0056] A method for preparing an enzyme electrode and an enzyme biosensor based on eggshell membrane modification, comprising the steps of:

[0057] (1) Use 1.0μm, 0.3μm and 0.05μm Al for the pyrolytic graphite electrode (PG) 2 o 3 The slurry is polished to a mirror surface on a suede polishing machine. After each polishing, the surface dirt is washed away with water, and then ultrasonically cleaned with ethanol and distilled water for 5 minutes respectively.

[0058] (2) The eggshell membrane is selected from the duck eggshell inner membrane, the duck eggshell inner membrane is peeled off and cleaned with water, cut into small pieces and placed on the surface of the polished pyrolytic graphite electrode, and put into an oven at room temperature, from room temperature Start to raise the temperature, increase the temperature by 3°C every 5min, raise the temperature to 70°C, and dry for 5h.

[0059] (3) Put the dried film-loaded pyrolytic graphite electrode into the quartz gla...

Embodiment 3

[0064] A method for preparing an enzyme electrode and an enzyme biosensor based on eggshell membrane modification, comprising the steps of:

[0065] (1) The carbon paste electrode (CPE) was coated with 1.0μm, 0.3μm and 0.05μm Al 2 o 3 The slurry was polished to a mirror surface on a suede polishing machine. After each polishing, the surface dirt was washed away with water, and then ultrasonically cleaned with ethanol and distilled water for 4 minutes respectively.

[0066] (2) The eggshell membrane is selected from the inner membrane of the quail eggshell, the inner membrane of the quail eggshell is peeled off and cleaned with water, cut into small pieces, placed on the surface of the polished carbon paste electrode, and put into an oven at room temperature, from The temperature began to rise at room temperature, and the temperature was raised by 5°C every 10 minutes, and the temperature was raised to 75°C, and dried for 4 hours.

[0067] (3) Put the dried film-supported car...

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PUM

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Abstract

The invention relates to an enzyme electrode, an enzyme biosensor as well as preparation methods and application of the enzyme electrode and the enzyme biosensor. The enzyme electrode comprises a base electrode, wherein a carbonized eggshell membrane is attached on the surface of the base electrode; nano-metal particles and enzyme are cured on the carbonized eggshell membrane. According to the enzyme electrode, a composite material of the eggshell membrane and the nano-metal particles is used as a carrier, enzyme molecules are effectively fixed and direct electron transfer between enzyme and the electrode is realized, thereby improving the catalytic activity of the enzyme molecules and the sensitivity of the sensor. Compared with the prior art, the enzyme electrode has the advantages that the eggshell membrane is used as a material so that reutilization of wastes is realized, and the preparation method is simple and low in cost, and electron transfer rate of the electrode and the enzyme is high; the enzyme biosensor is fast and accurate and high in detection sensitivity, stability and repeatability.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to an enzyme electrode, an enzyme biosensor and a preparation method and application thereof. Background technique [0002] In the field of biosensors, enzyme electrodes play an important role. The third-generation sensor widely studied at present uses the direct electron transfer between the enzyme itself and the electrode to complete the signal conversion without the introduction of mediators, which greatly improves the performance of the sensor. In order to achieve more effective direct electron transfer between the enzyme and the electrode, it is necessary to construct a suitable thin film electrode interface. Materials that facilitate direct electron transfer are preferred. [0003] A variety of electrochemical sensors modified with enzymes or enzyme-mediators have been used for substrate determination. In the development of biosensors, the immobilization of enzymes is a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48
Inventor 赵赫曹宏斌范壮军刘晨明
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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