35 results about "Enzyme Electrodes" patented technology

A temperature compensation method for an enzyme electrode by measuring an operating temperature of the enzyme electrode, measuring the current generated by the enzyme electrode determining a deviation in measurement between the current generated and a reference current at the operating temperature, determining an enzyme concentration corresponding to the measured current, and calibrating the enzyme concentration to compensate for the deviation in measurement.

The present invention relates to an enzyme electrode useful for estimation of cholesterol in aqueous medium, said electrode comprising: i. an electrically conductive base plate, ii. a film of sol gel derived material deposited thereon, said sol gel derived material of step b) being microencapsulated cholesterol oxidase with an electron mediator. The present invention also relates to a process for the preparation of an enzyme electrode by coating an immobilized cholesterol oxidase (ChOx) and mediator on a silicate sol gel by microencapsulation.

An enzyme electric couple catalyzing method for treating waste water containing phenol, aromatic amine and azo-dye is carried out by fixing horse-radish peroxidase or analog enzyme with enzyme activity on grinded pyrolyzed graphite electrode surface by surface activator and carboxy-carbon nano-pipe, making into solid-carried enzyme or analog enzyme electrode, taking solid-carried enzyme or analog enzyme electrode as cathode, taking graphite electrode as anode, pumping waste water containing phenol, aromatic amine and azo-dye into cathode chamber, inducing oxygen and air into cathode chamber to saturate, controlling cathode electric potential at -1.00--0.500V vs.SCE by constant potentiometer and constant-potential electrolyzing. It has fast degradation speed and high current efficiency.

The invention provides a glucose oxidase modified nitrogen doped titanium dioxide nanotube array enzyme electrode. The glucose oxidase modified nitrogen doped titanium dioxide nanotube array enzyme electrode comprises glucose oxidase, a nitrogen doped titanium dioxide nanotube array and a titanium substrate, wherein glucose oxidase is immobilized on the surface of a tube opening of the nitrogen doped titanium dioxide nanotube array which is longitudinally arrayed on the titanium substrate; the bottom of each nitrogen doped titanium dioxide nanotube is connected with the surface of the titanium substrate to form an integral structure. The invention also provides a preparation method of the enzyme electrode and application of the enzyme electrode to glucose electrochemical biosensors and glucose bio-enzyme fuel cells. The enzyme electrode is prepared by immobilizing glucose oxidase and a bovine serum albumin-glutaraldehyde crosslinking agent via crosslinking reaction with nitrogen doped titanium dioxide with favorable biocompatibility, hydrophilia and conductivity as an electrode substrate material. The preparation technology is simple, scale production can be realized with easiness in amplification, and the electrode has wide practical application value and prospect.

The present invention relates to an enzyme electrode including: a carbon particle; a metal particle held on the carbon particle, the metal particle having a catalytic activity against a redox reaction; a redox enzyme. The enzyme electrode of the present invention further includes a high-resistance particle enhancing an electrical resistance, the high-resistance particle being chemically stable. The high-resistance particle contains an inorganic substance, for example. The inorganic substance is aluminum oxide or smectite, for example.

The invention relates to a nanosilver modified titanium dioxide nanorod array enzyme electrode and a preparation method and application thereof. According to the method, carbon paper is utilized as asubstrate material. The method comprises the steps: firstly, growing a TiO2 nanorod array on the carbon paper; then soaking the carbon paper which the TiO2 nanorod array grows on into silver sol for 3to 15 hours, taking out, utilizing deionized water to clean the carbon paper and drying to obtain nanosilver modified titanium dioxide nanorod array; finally, utilizing bovine serum albumin-glutaraldehyde as a cross-linking agent and utilizing an improved cross-linking method to fix glucose oxidase on the surface of the nanosilver modified titanium dioxide nanorod array to form the nanosilver modified titanium dioxide nanorod array enzyme electrode. The prepared nanosilver modified titanium dioxide nanorod array enzyme electrode disclosed by the invention has better electrocatalytic activityand can be well applied to a glucose biosensor or an enzyme biological fuel cell.

The invention discloses a preparation method of a porous membrane layer. The preparation method comprises the steps that a precursor solution is placed in an airtight reaction chamber, and the precursor solution is subject to gelation to obtain porous gel. According to the preparation method, since the sol solution is subject to gelation in the airtight reaction chamber, pore collapse of the gel can be avoided, and the porous membrane layer with uniform pores and high porosity is obtained. The invention furthermore discloses an electrochemical sensor. The electrochemical sensor comprises a first electrode array and a second electrode array, wherein the first electrode array and the second electrode array share a first reference electrode, and the outer side face of the first reference electrode and the outer side face of an enzyme electrode are coated with hydrophobic porous membranes. The electrochemical sensor has high integrity; and since the two electrode arrays share the first reference electrode, the two electrode arrays can be arranged in the same detection channel, and the electrode structure of the senor is simplified. The invention furthermore discloses a preparation method of the electrochemical sensor. Through the preparation method of the electrochemical sensor, the preparation procedure is simplified, and preparation efficiency is high.

The invention relates to a temperature and pH value dual-stimulation responsive flexible laccase electrode and a construction method. According to the method, the dual-stimulation responsive laccase electrode is prepared on a flexible foam nickel conductive substrate through free radical electro-copolymerization, and laccase is fixed in a covalent bond manner, so that the electrode stability can be better met. One temperature-stimulation responsive monomer and two pH-stimulation responsive monomers are selected and subjected to free radical electro-copolymerization on the surface of the laccase electrode, and the prepared stimulation responsive laccase electrode has good on/off performance, has the catalytic peak current of 0.000771 A in a 20 mM glucose solution and has good catalytic performance. The electrode has the catalytic peak current only decreased by 6.49% in 10 times of on/off cycles and has good stability. The electrode can meet the cathode use requirement in EBFCs (enzymatic biofuel cells).

The invention provides a coenzyme factor compound, an enzyme electrode, an enzyme sensor as well as a preparation method and application thereof. The method comprises the following steps: chemically modifying a coenzyme factor small molecule NAD<+> non-active part through a chemical means, covalently connecting the chemically modified NAD<+> with a chitosan carrier to obtain an NAD<+>-chitosan compound, modifying a carbon nanotube on the surface of a substrate electrode, and detecting NADH by using the carbon nanotube as a substrate material; electrically depositing ABTS on the electrode, andrealizing NAD<+> in-situ regeneration by using the ABTS as an electron mediator; dropwise adding an NAD<+>-chitosan compound to realize fixation of NAD<+> on the surface of the electrode; connecting dehydrogenase to the chitosan carrier through glutaraldehyde cross-linking action to obtain a dehydrogenase electrode; and combining the immobilization and regeneration method of NAD<+> with differenttypes of dehydrogenases to prepare various dehydrogenase electrodes/biosensors. The coenzyme factor compound, the enzyme electrode, the enzyme sensor as well as the preparation method and applicationthereof have the great practical application value in the field of biosensor preparation.

A method for quantifying a substance, which method includes the steps of: introducing a sample containing a measurement target substance to a biosensor comprising an enzyme electrode comprising an electrode and an oxidoreductase placed on the electrode in a state where direct electron transfer with the electrode occurs, and a counter electrode; applying an AC voltage to the enzyme electrode to carry out impedance measurement; and calculating the substance concentration based on an index obtained by the impedance measurement; is provided.

Disclosed is an enzyme electrode in which an oxidation-reduction reaction proceeds by utilizing an enzyme as a catalyst. In the enzyme electrode, the enzyme immobilized thereon is so modified as to have increased affinity for a reaction substrate or an electron transfer mediator and/or an increased rate of reaction with the reaction substrate or the electron transfer mediator by adding or inserting at least one codon encoding a predetermined amino acid residue to a nucleotide sequence encoding the enzyme. On the enzyme electrode, an oxidation-reduction reaction can proceed with high efficiency. Therefore, an electric energy produced in the enzyme electrode can be output at a high level. For these reasons, the enzyme electrode can be used suitably in all types of fuel cells, biosensors and electronic devices.

The invention discloses an application of a nano enzyme electrode in detecting o-dihydroxybenzene. An electrode matrix of the nano-enzyme electrode is modified with a metal organic framework material, and a framework of the metal organic framework material contains a porphyrin ring. The nano-enzyme electrode realizes the detection of o-dihydroxybenzene in a solution to be detected, has high sensitivity and selectivity and a wide linear range, and is simple to manufacture and low in cost.

The invention discloses a preparation method and application of an enzyme electrode sensor for SAM detection. The preparation method is characterized by comprising the steps that 1, a glassy carbon electrode is polished with Al2O3 powder and cleaned by ethyl alcohol and water ultrasonic successively in an ultrasonic cleaner to obtain an activated glassy carbon electrode; 2, an electrochemical polymerization method is adopted on the activated glassy carbon electrode to obtain a poly-3,7-diamido-phenothiazine-5-onium modified electrode; 3, transmethylase is fixed to the poly-3,7-diamido-phenothiazine-5-onium modified electrode in a crosslinking mode to obtain a fixed transmethylase electrode sensor. SAM in a sample can be rapidly detected through the electrode, the method is high in sensitivity, good in selectivity, short in response time, low in interference and better than other detection methods, and is an SAM determination method which is simple, rapid, convenient and easy to implement. The obtained fixed transmethylase electrode sensor is low in cost, simple in preparation technology and good in specificity and has the potential for achieving automatic in-situ measurement.

The invention discloses a cationic dipeptide and gold hybridized microsphere and a preparation method and application thereof. The microsphere is prepared by biocompatible peptide biomolecules and chloroauric acid through reaction under conditions of stirring and heating. The preparation method comprises the following steps of: dissolving the peptide through a solvent; adding the dissolved peptideinto water solution of the chloroauric acid; stirring the mixture for a certain time; and heating the mixture again to form the microsphere. The preparation method of the microsphere has the characteristics of being simple and easy to do, mild in condition and good in repeatability; the microsphere has good stability and biocompatibility; the doping of gold in the prepared microsphere is capableof improving the conductivity of the microsphere and catalytic performance of the microsphere relative to biological detection application, and effectively improving the performance of electrochemicalsensors; the cationic dipeptide and gold hybridized microsphere prepared by the method can be applied to biological sensing analysis; and an obtained enzyme electrode can be used for cholesterol detection, and has relatively high sensitivity and rapid response performance.

The invention relates to a preparation method of a high-performance biosensor based on graphene aerogel. The preparation method is characterized by comprising the following steps: (1) preparing graphene aerogel/platinum nanoparticles: adding graphene oxide powder into de-ionized water; adding chloroplatinic acid and sealing in a polytetrafluoroethylene high-pressure reaction kettle; after reacting, naturally cooling to room temperature and taking out prepared graphene hydrogel/platinum nanoparticles; then treating by utilizing a CO2 supercritical extraction drying method to obtain the grapheneaerogel/platinum nanoparticles; (2) preparing an enzyme electrode: taking the graphene aerogel/platinum nanoparticles, adding absolute ethyl alcohol and adding a biological enzyme to prepare a graphene aerogel/platinum nanoparticle/biological enzyme solution; dropwise adding the solution to the surface of a glassy carbon electrode; dropwise adding a Nafion solution to obtain a graphene aerogel/platinum nanoparticle/biological enzyme glassy carbon electrode to replace a basic structure unit immobilized enzyme membrane of a traditional biosensor, so as to obtained the high-performance biosensorbased on the graphene aerogel.

The invention relates to a measuring method of the quality of inosine and belongs to the biosensor technical field. The preparing method is that: nucleoside phosphorylase and hypoxanthine dehydrogenase are co-immobilized and combined with the electrode of H202 to form an inosine enzyme electrode biosensor; phosphate buffer solution of 0.1 mmol.L< -1 >PH7.2 is adopted; the amount of hypoxanthine contained in the standard solution is 268mg.L< -1 >; 25ul standard solution is injected into a reaction tank for 25 seconds, then the instrument automatically shows the base liveness of enzyme membrane; a calibrate key is pressed to calibrate 100, and the sample can be measured after automatic washing.

A blood measuring instrument is provided for measuring a specific component of blood. The instrument includes a plate-like chip (2) for sucking sampled blood and a main detecting unit (1) provided with a connector (3) into which the chip (2) is plugged. The chip (2) has, on its one side, an enzyme electrode section for passing a response current upon reaction with a specific component of the blood, and lead terminals connected to the enzyme electrode section. The main detecting unit (1) has, within the connector, two sets of connection terminals (21,22) engageable with the lead terminals on the chip (2). These two sets of the connection terminals (21,22) are held in facing relation to each other. It is possible to insert the chip (2) into the connector (3) of the main detecting unit (1) whether one surface of the chip faces upward or downward. The lead terminals of the chip are properly connected to either one of the two sets of the connection terminals (21, 22) facing each other in the connector, thereby enabling desired measurement.