37 results about "Enzyme Electrodes" patented technology

A non-mediated enzyme electrode comprises a base substrate (2) on which is provided an electrically conductive base layer (8) comprising finely divided platinum group metal or oxide bonded together by a resin; a top layer on the base layer (8), the top layer comprising a buffer. A catalytically active quantity of an oxidoreductase enzyme is provided in at least one of the base layer and the top layer. The invention also provides a biosensor (20) which includes an enzyme electrode, and methods of manufacturing the enzyme electrode and biosensor.

A temperature compensation method for an enzyme electrode by measuring an operating temperature of the enzyme electrode, measuring the current generated by the enzyme electrode determining a deviation in measurement between the current generated and a reference current at the operating temperature, determining an enzyme concentration corresponding to the measured current, and calibrating the enzyme concentration to compensate for the deviation in measurement.

The present invention relates to an enzyme electrode useful for estimation of cholesterol in aqueous medium, said electrode comprising: i. an electrically conductive base plate, ii. a film of sol gel derived material deposited thereon, said sol gel derived material of step b) being microencapsulated cholesterol oxidase with an electron mediator. The present invention also relates to a process for the preparation of an enzyme electrode by coating an immobilized cholesterol oxidase (ChOx) and mediator on a silicate sol gel by microencapsulation.

An enzyme electrode comprises a conductive member and an enzyme, wherein a first mediator and a second mediator are immobilized by a carrier onto the conductive member, the first mediator and the second mediator having different redox potentials (reduction-oxidation potentials). A sensor employs the enzyme electrode as a detection portion for detecting a substance. A fuel cell employs the enzyme electrode as at least one of anode and a cathode. An electrochemical reactor employs the enzyme electrode as a reaction electrode.

An enzyme electric couple catalyzing method for treating waste water containing phenol, aromatic amine and azo-dye is carried out by fixing horse-radish peroxidase or analog enzyme with enzyme activity on grinded pyrolyzed graphite electrode surface by surface activator and carboxy-carbon nano-pipe, making into solid-carried enzyme or analog enzyme electrode, taking solid-carried enzyme or analog enzyme electrode as cathode, taking graphite electrode as anode, pumping waste water containing phenol, aromatic amine and azo-dye into cathode chamber, inducing oxygen and air into cathode chamber to saturate, controlling cathode electric potential at -1.00--0.500V vs.SCE by constant potentiometer and constant-potential electrolyzing. It has fast degradation speed and high current efficiency.

The invention provides a glucose oxidase modified nitrogen doped titanium dioxide nanotube array enzyme electrode. The glucose oxidase modified nitrogen doped titanium dioxide nanotube array enzyme electrode comprises glucose oxidase, a nitrogen doped titanium dioxide nanotube array and a titanium substrate, wherein glucose oxidase is immobilized on the surface of a tube opening of the nitrogen doped titanium dioxide nanotube array which is longitudinally arrayed on the titanium substrate; the bottom of each nitrogen doped titanium dioxide nanotube is connected with the surface of the titanium substrate to form an integral structure. The invention also provides a preparation method of the enzyme electrode and application of the enzyme electrode to glucose electrochemical biosensors and glucose bio-enzyme fuel cells. The enzyme electrode is prepared by immobilizing glucose oxidase and a bovine serum albumin-glutaraldehyde crosslinking agent via crosslinking reaction with nitrogen doped titanium dioxide with favorable biocompatibility, hydrophilia and conductivity as an electrode substrate material. The preparation technology is simple, scale production can be realized with easiness in amplification, and the electrode has wide practical application value and prospect.

The invention provides an enzyme electrode capable of detecting aiming objects with high sensitivity, high speed, good stability and long service life, and an enzyme sensor using the enzyme electrode. The enzyme electrode (1) comprises: an electrode (2); a carbon nanotube layer (L) including a plurality of carbon nanotubes (3) extending directly from the electrode (2) and / or a metallic catalyst immobilized on the electrode (2); and an enzyme immobilized in the carbon nanotube layer (L) by being sandwiched between the carbon nanotubes (3).

The invention provides a method and a reactor for removing organic matters by enzyme electrode coupled electroflocculation. In the method, the same electrochemical system in combination with an enzyme-modified electrode and electroflocculation synergistically removes organic matters in water; certain electrochemical parameters are controlled, so that polymerization reaction is performed on hydrogen peroxide generated by cathode reaction and an enzyme electrode-catalyzed organic matter; after the reaction, the organic product enters an anode chamber through a pump; the organic product and different electroflocculation hydrolyzates are precipitated and removed through compression of double electric layers, adsorption and electric neutralization, and precipitation netting capture and other coagulating / flocculating effects; and ultimately, the aim of removing the organic matters through cathode enzyme catalytic polymerization and anode electroflocculation synergistically is achieved. Themethod and the reactor have a strong selectivity for low-concentration toxic organic pollutants and a good effect in removing small-molecular hydrophilic organic matters; the enzyme adding amount is low; the cathode and the anode are fully used; the cost is reduced; the operation is simple; the applicable is wide; therefore, the method and the reactor is applicable to a variety of water and wastewater containing the organic matters, and contaminated water.

The present invention relates to an enzyme electrode including: a carbon particle; a metal particle held on the carbon particle, the metal particle having a catalytic activity against a redox reaction; a redox enzyme. The enzyme electrode of the present invention further includes a high-resistance particle enhancing an electrical resistance, the high-resistance particle being chemically stable. The high-resistance particle contains an inorganic substance, for example. The inorganic substance is aluminum oxide or smectite, for example.

An enzyme electrode quantitative alcohol test strip belongs to the biosensor field. An electrode and an electric circuit are printed on the basal plate of the test paper, a lead wire connected with a readout instrument is printed on one end of the basal plate, the electrode is a porous layer which is printed on a basal plate conductive layer, and biological enzyme, medium and protective agent are fixed on the electrode. The invention adopts the enzyme catalysis oxidation reduction reaction and electrochemical principle and prepares the test paper through printing. As long as the test paper provided by the invention touches test solution, a quantitative test result can be visually and conveniently read out on a corresponding matching testing apparatus. The enzyme electrode quantitative alcohol test strip with simple preparation method facilitates large-scale production.

The invention relates to a nanosilver modified titanium dioxide nanorod array enzyme electrode and a preparation method and application thereof. According to the method, carbon paper is utilized as asubstrate material. The method comprises the steps: firstly, growing a TiO2 nanorod array on the carbon paper; then soaking the carbon paper which the TiO2 nanorod array grows on into silver sol for 3to 15 hours, taking out, utilizing deionized water to clean the carbon paper and drying to obtain nanosilver modified titanium dioxide nanorod array; finally, utilizing bovine serum albumin-glutaraldehyde as a cross-linking agent and utilizing an improved cross-linking method to fix glucose oxidase on the surface of the nanosilver modified titanium dioxide nanorod array to form the nanosilver modified titanium dioxide nanorod array enzyme electrode. The prepared nanosilver modified titanium dioxide nanorod array enzyme electrode disclosed by the invention has better electrocatalytic activityand can be well applied to a glucose biosensor or an enzyme biological fuel cell.

The invention discloses a preparation method of a porous membrane layer. The preparation method comprises the steps that a precursor solution is placed in an airtight reaction chamber, and the precursor solution is subject to gelation to obtain porous gel. According to the preparation method, since the sol solution is subject to gelation in the airtight reaction chamber, pore collapse of the gel can be avoided, and the porous membrane layer with uniform pores and high porosity is obtained. The invention furthermore discloses an electrochemical sensor. The electrochemical sensor comprises a first electrode array and a second electrode array, wherein the first electrode array and the second electrode array share a first reference electrode, and the outer side face of the first reference electrode and the outer side face of an enzyme electrode are coated with hydrophobic porous membranes. The electrochemical sensor has high integrity; and since the two electrode arrays share the first reference electrode, the two electrode arrays can be arranged in the same detection channel, and the electrode structure of the senor is simplified. The invention furthermore discloses a preparation method of the electrochemical sensor. Through the preparation method of the electrochemical sensor, the preparation procedure is simplified, and preparation efficiency is high.

The invention relates to a temperature and pH value dual-stimulation responsive flexible laccase electrode and a construction method. According to the method, the dual-stimulation responsive laccase electrode is prepared on a flexible foam nickel conductive substrate through free radical electro-copolymerization, and laccase is fixed in a covalent bond manner, so that the electrode stability can be better met. One temperature-stimulation responsive monomer and two pH-stimulation responsive monomers are selected and subjected to free radical electro-copolymerization on the surface of the laccase electrode, and the prepared stimulation responsive laccase electrode has good on / off performance, has the catalytic peak current of 0.000771 A in a 20 mM glucose solution and has good catalytic performance. The electrode has the catalytic peak current only decreased by 6.49% in 10 times of on / off cycles and has good stability. The electrode can meet the cathode use requirement in EBFCs (enzymatic biofuel cells).

A flux limiting layer for an intravenous amperometric biosensor is formed on a substrate to limit a diffusion rate of an analyte from blood to an enzyme electrode. The layer may be formed from ethylene vinylacetate (EVA) dissolved in a solvent such as paraxylene, spray-coated to cover a portion of the electrode, and cured to seal the electrode to the substrate. In a glucose sensor having glucose oxidase disposed on the electrode, thickness and concentration of the EVA layer are optimized to promote a linear output of electrode current as a function of blood glucose concentration.

The invention provides a coenzyme factor compound, an enzyme electrode, an enzyme sensor as well as a preparation method and application thereof. The method comprises the following steps: chemically modifying a coenzyme factor small molecule NAD<+> non-active part through a chemical means, covalently connecting the chemically modified NAD<+> with a chitosan carrier to obtain an NAD<+>-chitosan compound, modifying a carbon nanotube on the surface of a substrate electrode, and detecting NADH by using the carbon nanotube as a substrate material; electrically depositing ABTS on the electrode, andrealizing NAD<+> in-situ regeneration by using the ABTS as an electron mediator; dropwise adding an NAD<+>-chitosan compound to realize fixation of NAD<+> on the surface of the electrode; connecting dehydrogenase to the chitosan carrier through glutaraldehyde cross-linking action to obtain a dehydrogenase electrode; and combining the immobilization and regeneration method of NAD<+> with differenttypes of dehydrogenases to prepare various dehydrogenase electrodes / biosensors. The coenzyme factor compound, the enzyme electrode, the enzyme sensor as well as the preparation method and applicationthereof have the great practical application value in the field of biosensor preparation.

The present invention relates to an enzyme electrode for transporting electric charge formed by an oxidation-reduction reaction in an enzyme to a conductive member at a high speed. Specifically, the enzyme electrode has a π-conjugated metal complex molecule immobilized on the conductive member and the enzyme immobilized on the conductive member through the π-conjugated metal complex molecule.

A method for quantifying a substance, which method includes the steps of: introducing a sample containing a measurement target substance to a biosensor comprising an enzyme electrode comprising an electrode and an oxidoreductase placed on the electrode in a state where direct electron transfer with the electrode occurs, and a counter electrode; applying an AC voltage to the enzyme electrode to carry out impedance measurement; and calculating the substance concentration based on an index obtained by the impedance measurement; is provided.

An enzyme electrode having excellent sensitivity, excellent stability, and a longer operating life, and an enzyme sensor using the enzyme electrode are provided. The enzyme electrode includes an electrode 2, a mesoporous silica material 3 formed on the electrode 2, and enzyme 4 immobilized in a small cavity of the mesoporous silica material 3. The size of the small cavity of the mesoporous silica material 3 is set to be 0.5 to 2.0 times the size of the enzyme 4.

Disclosed is an enzyme electrode in which an oxidation-reduction reaction proceeds by utilizing an enzyme as a catalyst. In the enzyme electrode, the enzyme immobilized thereon is so modified as to have increased affinity for a reaction substrate or an electron transfer mediator and / or an increased rate of reaction with the reaction substrate or the electron transfer mediator by adding or inserting at least one codon encoding a predetermined amino acid residue to a nucleotide sequence encoding the enzyme. On the enzyme electrode, an oxidation-reduction reaction can proceed with high efficiency. Therefore, an electric energy produced in the enzyme electrode can be output at a high level. For these reasons, the enzyme electrode can be used suitably in all types of fuel cells, biosensors and electronic devices.

The invention discloses a novel cloud intelligent insulin pump system which comprises an intelligent insulin pump body, a cloud storage station and a remote monitoring terminal, wherein the intelligent insulin pump body can transmit physical sign data to the cloud storage station; the cloud storage station can transmit the physical sign data to the remote monitoring terminal; and the intelligent insulin pump body comprises a minitype thrust electric pump, a medicine storage capsule cabin, a needle head matrix, a CPLD controller, an enzyme electrode sensor, a wireless module, a key module, a liquid crystal module, an alarm module and a battery. Due to the special design of the needle head, medicine injection is realized while stimulation of the needle head on rich subcutaneous nerves, and pain and stimulation are avoided; furthermore, a large number of physical sign information can be acquired and the acquired data is subjected to cloud sharing, so that supervision of medical workers and monitoring of the body condition are facilitated, a multiple abnormity alarm mechanism enables the system to be safe and reliable, and the system has high response speed, realizes quick adjustment and has a good regulation and control effect on the blood sugar of patients.

An enzyme electrode and its producing method are disclosed. The method includes providing a substrate having a carbon surface; forming a gold surface on the carbon surface and forming an electrode; modifying the gold surface by L-cysteine; modifying the gold surface by N,N′-dicyclohexylcarbodiimide; and chemically bonding the modified gold surface with a glucose oxidase.

The invention discloses an application of a nano enzyme electrode in detecting o-dihydroxybenzene. An electrode matrix of the nano-enzyme electrode is modified with a metal organic framework material, and a framework of the metal organic framework material contains a porphyrin ring. The nano-enzyme electrode realizes the detection of o-dihydroxybenzene in a solution to be detected, has high sensitivity and selectivity and a wide linear range, and is simple to manufacture and low in cost.

The invention discloses a preparation method and application of an enzyme electrode sensor for SAM detection. The preparation method is characterized by comprising the steps that 1, a glassy carbon electrode is polished with Al2O3 powder and cleaned by ethyl alcohol and water ultrasonic successively in an ultrasonic cleaner to obtain an activated glassy carbon electrode; 2, an electrochemical polymerization method is adopted on the activated glassy carbon electrode to obtain a poly-3,7-diamido-phenothiazine-5-onium modified electrode; 3, transmethylase is fixed to the poly-3,7-diamido-phenothiazine-5-onium modified electrode in a crosslinking mode to obtain a fixed transmethylase electrode sensor. SAM in a sample can be rapidly detected through the electrode, the method is high in sensitivity, good in selectivity, short in response time, low in interference and better than other detection methods, and is an SAM determination method which is simple, rapid, convenient and easy to implement. The obtained fixed transmethylase electrode sensor is low in cost, simple in preparation technology and good in specificity and has the potential for achieving automatic in-situ measurement.

The invention discloses a cationic dipeptide and gold hybridized microsphere and a preparation method and application thereof. The microsphere is prepared by biocompatible peptide biomolecules and chloroauric acid through reaction under conditions of stirring and heating. The preparation method comprises the following steps of: dissolving the peptide through a solvent; adding the dissolved peptideinto water solution of the chloroauric acid; stirring the mixture for a certain time; and heating the mixture again to form the microsphere. The preparation method of the microsphere has the characteristics of being simple and easy to do, mild in condition and good in repeatability; the microsphere has good stability and biocompatibility; the doping of gold in the prepared microsphere is capableof improving the conductivity of the microsphere and catalytic performance of the microsphere relative to biological detection application, and effectively improving the performance of electrochemicalsensors; the cationic dipeptide and gold hybridized microsphere prepared by the method can be applied to biological sensing analysis; and an obtained enzyme electrode can be used for cholesterol detection, and has relatively high sensitivity and rapid response performance.

The invention relates to a preparation method of a high-performance biosensor based on graphene aerogel. The preparation method is characterized by comprising the following steps: (1) preparing graphene aerogel / platinum nanoparticles: adding graphene oxide powder into de-ionized water; adding chloroplatinic acid and sealing in a polytetrafluoroethylene high-pressure reaction kettle; after reacting, naturally cooling to room temperature and taking out prepared graphene hydrogel / platinum nanoparticles; then treating by utilizing a CO2 supercritical extraction drying method to obtain the grapheneaerogel / platinum nanoparticles; (2) preparing an enzyme electrode: taking the graphene aerogel / platinum nanoparticles, adding absolute ethyl alcohol and adding a biological enzyme to prepare a graphene aerogel / platinum nanoparticle / biological enzyme solution; dropwise adding the solution to the surface of a glassy carbon electrode; dropwise adding a Nafion solution to obtain a graphene aerogel / platinum nanoparticle / biological enzyme glassy carbon electrode to replace a basic structure unit immobilized enzyme membrane of a traditional biosensor, so as to obtained the high-performance biosensorbased on the graphene aerogel.

The invention relates to a measuring method of the quality of inosine and belongs to the biosensor technical field. The preparing method is that: nucleoside phosphorylase and hypoxanthine dehydrogenase are co-immobilized and combined with the electrode of H202 to form an inosine enzyme electrode biosensor; phosphate buffer solution of 0.1 mmol.L< -1 >PH7.2 is adopted; the amount of hypoxanthine contained in the standard solution is 268mg.L< -1 >; 25ul standard solution is injected into a reaction tank for 25 seconds, then the instrument automatically shows the base liveness of enzyme membrane; a calibrate key is pressed to calibrate 100, and the sample can be measured after automatic washing.

The invention discloses an enterotoxin bioelectrode, which comprises: a conductive rod-shaped substrate, a platinum black plating layer electroplated on the outer surface of the rod end of the substrate, and an oxidase film fixed on the platinum black plating layer. On the oxidase membrane, an enterotoxin antibody membrane is coupled with glutaraldehyde, and the matrix, platinum black coating, oxidase membrane, and enterotoxin antibody membrane constitute a device for specifically detecting the content of enterotoxin antigen in the sample solution. Enterotoxin enzyme immunoelectrode. The preparation steps are as follows: (1) matrix pretreatment; (2) preparation of platinum black electrode; (3) preparation of enzyme electrode; (4) preparation of enterotoxin enzyme immune electrode. The order of magnitude of the electrode used to detect the sample concentration is ug / ml. It has both the catalytic amplification effect of the enzyme electrode and the specificity of the molecular recognition reaction between the antigen and the antibody of the immune electrode. It has biological activity, high sensitivity and stability, and has the advantages of simple sample preparation, small size, and portability. The test time is short, the response speed is fast, the detection sensitivity is high, and the characteristics are easy to control.