Enzymic electrode method quantitative ethanol test strip

A method of quantitative ethanol and enzyme electrode technology, applied in the field of biosensing, can solve the problems of high production cost of ethanol tester, resistance of subjects, and hygiene problems, etc., achieve intuitive and convenient results, reduce process requirements, and improve accuracy high effect

Inactive Publication Date: 2008-08-06
QUICKING BIOTECH
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Problems solved by technology

The main disadvantage of this method is that the production cost of the ethanol tester is relatively high, and there are hygiene problems in repeated use, which is not conducive to rapid judgment of ethanol concentration on the spot and large-scale promotion. Hygienic problems are likely to cause resistance to the subjects

Method used

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  • Enzymic electrode method quantitative ethanol test strip

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Experimental program
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Embodiment

[0013] Example: polyester sheet material, pre-cut into 80×300 mm substrates for later use.

[0014] (1) Add 15% hydroxymethyl cellulose, 20% electronic conductive carrier material potassium ferricyanide and diluent in the conductive carbon ink, mix for subsequent use;

[0015] (2) Install the electrode template on the stationary machine for standby;

[0016] (3) Alcohol oxidase with an activity of 3000 international units per milliliter is added to 120 millimoles per liter of phosphate buffered water, and at the same time, 600 milligrams of potassium ferricyanide per liter and 15% hydroxymethyl cellulose are added to fully dissolve Put it aside in the refrigerator at 4°C for later use.

[0017] Add conductive paste to the above-mentioned fixing machine to fix the conductive circuit; after drying, add the above-mentioned mixed conductive carbon ink to the automatic fixing machine to cover the previously printed silver ink circuit to make an electrode. The printed electrodes n...

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Abstract

An enzyme electrode quantitative alcohol test strip belongs to the biosensor field. An electrode and an electric circuit are printed on the basal plate of the test paper, a lead wire connected with a readout instrument is printed on one end of the basal plate, the electrode is a porous layer which is printed on a basal plate conductive layer, and biological enzyme, medium and protective agent are fixed on the electrode. The invention adopts the enzyme catalysis oxidation reduction reaction and electrochemical principle and prepares the test paper through printing. As long as the test paper provided by the invention touches test solution, a quantitative test result can be visually and conveniently read out on a corresponding matching testing apparatus. The enzyme electrode quantitative alcohol test strip with simple preparation method facilitates large-scale production.

Description

Technical field [0001] The invention relates to an enzyme electrode quantitative test strip, in particular to an ethanol test strip, which belongs to the technical field of biosensing. Background technique [0002] At present, most of the ethanol content test products on the market adopt the design method of ethanol fuel cell, and use the relationship between the ethanol concentration and the output result of the fuel cell to judge the ethanol concentration. The main disadvantage of this method is that the production cost of the ethanol tester is relatively high, and there are sanitary problems in repeated use, which is not conducive to rapid judgment of ethanol concentration on the spot and large-scale promotion. Sanitary problems are likely to cause resistance among the subjects. Contents of the invention [0003] The purpose of the present invention is to overcome the deficiencies in the existing products and provide a quantitative test strip for ethanol by the enzyme e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N33/98
Inventor 王学生冯吉
Owner QUICKING BIOTECH
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