Preparation method of high-performance biosensor based on graphene aerogel

A graphene aerogel, biosensor technology, applied in the field of high-performance biosensor preparation, can solve the problems of low sensitivity, expensive processing, poor specificity, etc.

Inactive Publication Date: 2018-06-15
JINAN DEV ZONE XINGHUO SCI & TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to effectively prevent these diseases caused by abnormal concentrations of human metabolites, people need a simple and effective detection method, while the existing medical detection mostly requires expensive equipment, professional operations and complex processing...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Preparation of graphene oxide: Use 25ml of concentrated sulfuric acid with a volume fraction of more than 98%, add 1g of potassium nitrate, stir in an ice bath, add 2g of graphite and stir in an ice bath for 30min, slowly add 20g of 50wt% potassium permanganate aqueous solution, ice Stir in a bath for 30 minutes, transfer to a 20°C water bath and stir for 30 minutes, slowly add 200ml of deionized water, transfer to a 90°C water bath and stir for 10 minutes, then slowly add 500ml of deionized water and 40ml of 20% hydrogen peroxide and stir for 10 minutes. Until the color of the mixture turns bright yellow, the mixture is centrifuged and washed to neutrality, and the graphene oxide powder is obtained after drying.

[0015] (2) Preparation of graphene hydrogel / platinum nanoparticles: Take 100mg of graphene oxide powder, add 30ml of deionized water, use an ultrasonic stirrer to ultrasonically stir for about 0.5h, then heat the solution in a water bath for about 0.5h, an...

Embodiment 2

[0023] (1) Preparation of graphene oxide: Use 50ml of concentrated sulfuric acid with a volume fraction above 98%, add 1.5g of potassium nitrate, stir in an ice bath, add 2g of graphite in an ice bath and stir for 45min, slowly add 25g of 50wt% potassium permanganate aqueous solution, Stir in an ice bath for 45 minutes, transfer to a 25°C water bath and stir for 45 minutes, slowly add 200ml of deionized water, transfer to a 100°C water bath and stir for 20 minutes, then slowly add 500ml of deionized water and 40ml of 25% hydrogen peroxide and stir for 10 minutes. Until the color of the mixture turns bright yellow, the mixture is centrifuged and washed to neutrality, and the graphene oxide powder is obtained after drying.

[0024] (2) Preparation of graphene hydrogel / platinum nanoparticles: Take 200mg of graphene oxide powder, add 100ml of deionized water, use an ultrasonic stirrer to ultrasonically stir for about 0.5h, then heat the solution in a water bath for about 0.5h, and ...

Embodiment 3

[0032](1) Preparation of graphene oxide: Use 100ml of concentrated sulfuric acid with a volume fraction of more than 98%, add 2g of potassium nitrate, stir in an ice bath, add 2g of graphite and stir in an ice bath for 60min, slowly add 30g of 50wt% potassium permanganate aqueous solution, ice Stir in a bath for 60 minutes, transfer to a 30°C water bath and stir for 60 minutes, slowly add 200ml of deionized water, transfer to a 110°C water bath and stir for 30 minutes, then slowly add 500ml of deionized water and 40ml of 30% hydrogen peroxide and stir for 10 minutes. Until the color of the mixture turns bright yellow, the mixture is centrifuged and washed to neutrality, and the graphene oxide powder is obtained after drying.

[0033] (2) Preparation of graphene hydrogel / platinum nanoparticles: Take 300mg of graphene oxide powder, add 100ml of deionized water, use an ultrasonic stirrer to ultrasonically stir for about 0.5h, then heat the solution in a water bath for about 0.5h, ...

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PUM

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Abstract

The invention relates to a preparation method of a high-performance biosensor based on graphene aerogel. The preparation method is characterized by comprising the following steps: (1) preparing graphene aerogel/platinum nanoparticles: adding graphene oxide powder into de-ionized water; adding chloroplatinic acid and sealing in a polytetrafluoroethylene high-pressure reaction kettle; after reacting, naturally cooling to room temperature and taking out prepared graphene hydrogel/platinum nanoparticles; then treating by utilizing a CO2 supercritical extraction drying method to obtain the grapheneaerogel/platinum nanoparticles; (2) preparing an enzyme electrode: taking the graphene aerogel/platinum nanoparticles, adding absolute ethyl alcohol and adding a biological enzyme to prepare a graphene aerogel/platinum nanoparticle/biological enzyme solution; dropwise adding the solution to the surface of a glassy carbon electrode; dropwise adding a Nafion solution to obtain a graphene aerogel/platinum nanoparticle/biological enzyme glassy carbon electrode to replace a basic structure unit immobilized enzyme membrane of a traditional biosensor, so as to obtained the high-performance biosensorbased on the graphene aerogel.

Description

technical field [0001] The invention relates to the field of biosensor preparation, in particular to a method for preparing a high-performance biosensor based on graphene aerogel. Background technique [0002] Clinical medicine has shown that many human diseases are related to the abnormal concentration of metabolites in the human body. For example, excessive concentration of uric acid can cause ventilation, and high concentration of cholesterol can cause cardiovascular and cerebrovascular diseases. In order to effectively prevent these diseases caused by the abnormal concentration of human metabolites, people need a simple and effective detection method, and the existing medical detection mostly requires expensive instruments and equipment, professional operations and complicated processing procedures. At present, the common biosensors on the market have disadvantages such as low sensitivity, poor specificity, low interference ability, and slow response speed, so the prepar...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 车春玲
Owner JINAN DEV ZONE XINGHUO SCI & TECH RES INST
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