21 results about "Enzyme membrane" patented technology

The utility model provides a membrane separation system for an active biological enzyme, and belongs to the technical field of membrane separators. The membrane separation system for the active biological enzyme comprises a membrane separator and a heating and cooling assembly. The heating and cooling assembly comprises a first outer tank, the first outer tank is arranged on the outer side of the membrane separator, the inner wall of the first outer tank and the outer wall of the membrane separator form a cooling cavity, the bottom of the first outer tank is communicated with a cold injection pipe, a second outer tank is arranged outside the first outer tank, and the second outer tank is communicated with the cooling cavity. A heating cavity is formed by the inner wall of the second outer tank and the outer wall of the first outer tank, and an electric heater is installed in the heating cavity. Through the cooling cavity and the heating cavity formed by the first outer tank and the second outer tank, the temperature of the membrane separator can be reduced, so that the temperature can be adjusted according to the temperature requirement during actual biological enzyme membrane separation, and the biological enzyme is prevented from being inactivated or degraded during membrane separation.

The application belongs to the technical field of natural substance separation and extraction, and particularly relates to a kind of Haematococcus pluvialis multi-product cascade separation method based on eutectic solvent sequential extraction and enzyme membrane reactor coupling, comprising the following steps: (1) cell wall weakening pretreatment step; (2) water-soluble component extraction step; (3) pigment component extraction step; (4) astaxanthin extraction step; (5) enzyme conversion step. Through the synergistic effect of eutectic solvent sequential extraction and enzyme membrane reactor, efficient, green and continuous cascade separation of water-soluble components, pigments, astaxanthin and xylo-oligosaccharides in Haematococcus pluvialis is realized, and product purity and resource utilization are improved.

This invention relates to an immobilized enzyme membrane, its preparation method, and its application in the preparation of rare sugars, belonging to the field of enzyme catalysis technology. This invention prepares an immobilized enzyme membrane by controlling the surface charge of the membrane and then crosslinking it with RDPE and TMC. The positively charged membrane is more conducive to the loading of negatively charged RDPE. This immobilized enzyme membrane greatly improves the stability of the enzyme and enables its reuse and recovery. Free enzyme membranes and immobilized enzyme membranes were tested in positive osmosis mode. The immobilized enzyme membrane can achieve in-situ separation of products and can break the limitations of reaction equilibrium.

The invention belongs to the technical field of photosensitive enzyme membrane reactors, and particularly relates to a transition metal-based porphyrin photosensitive enzyme membrane reactor based on 2-methylimidazole regulation and a preparation method and application thereof. The porphyrin photosensitive enzyme membrane reactor comprises a transition metal-based porphyrin photosensitive membrane doped with 2-methylimidazole and light decarboxylase loaded on the photosensitive membrane, and the transition metal-based porphyrin photosensitive membrane doped with 2-methylimidazole is a tetra (4-hydroxyphenyl) zinc porphyrin photosensitive membrane doped with 2-methylimidazole; the light decarboxylase is fatty acid light decarboxylase of chlorella variants NC64A (chlorella vulgaris NC64A). According to the transition metal-based porphyrin photosensitive enzyme membrane reactor based on 2-methylimidazole regulation and control, the technical problems that the enzyme stability is poor and the recovery is difficult due to the fact that a traditional porphyrin membrane immobilized enzyme is easy to fall off are effectively solved; and the transition metal-based porphyrin photosensitive enzyme membrane enables the catalytic efficiency of the immobilized photo-decarboxylase to be improved to a great extent.

The application provides a method for continuously producing chitooligosaccharide with specific polymerization degree by using an immobilized enzyme membrane reactor. The method is as follows: 1. preparing a PLGA nanofiber-temperature-sensitive hydrogel composite carrier, fixing the carrier in the inner wall of a ceramic membrane to obtain a composite carrier membrane; 2. assembling the composite carrier membrane into a folding enzyme membrane reactor with temperature control, pressure monitoring and material switching units; 3. introducing enzyme solution to fix chitosanase, and then introducing pretreated chitosan substrate to catalyze the reaction; 4. monitoring the polymerization degree on line to control the reaction process, washing the reactor after the reaction to realize continuous production, and concentrating and spray drying the product to obtain the target product. The application fuses the characteristics of multiple carriers to construct a “rigid support-flexible regulation” structure: the ceramic provides stable mechanical support, the temperature-sensitive hydrogel adjusts the swelling degree with temperature, the enzyme active site is exposed at low temperature to improve the efficiency, and the product is released by shrinking at a later stage, so that the problem of product retention and side reaction is solved.

This invention relates to a biochemical sample processing and analysis device, comprising a housing and a display module, a sample processing module, a detection and analysis module, and a control system disposed within the housing. The sample processing module includes a sample box, a stock solution tank, a dilution tank, and a pipetting platform located on the upper part of the housing. A temperature control module is disposed below the sample box. The pipetting platform includes a three-dimensional motion mechanism, a pipette, and a pipetting pump. The detection and analysis module includes one or more of a photoelectric detection component, an ion detection component, and an enzyme membrane detection component. The control system is communicatively connected to the sample processing module, the detection and analysis module, and the display module.

The invention discloses a method for extracting collagen peptide from fish byproducts, which comprises the following steps: putting minced fish bones into an EDTA (Ethylene Diamine Tetraacetic Acid) solution for decalcification treatment to obtain decalcified minced fish bones; putting the minced fish skin into an ethanol solution for degreasing treatment to obtain degreased minced fish skin; putting the minced fish scales into an organic acid solution for deashing treatment to obtain deashed minced fish scales; mixing the decalcified minced fish bone, the degreased minced fish skin and the deashed minced fish scale, then adding a compound enzyme for enzymolysis treatment, and filtering to obtain an enzymolysis mixture; recovering the compound enzyme in the enzymolysis mixture to obtain enzymolysis liquid, then sequentially performing microfiltration, ultrafiltration and nanofiltration to obtain nanofiltration interception liquid, and then desalting and concentrating to obtain collagen peptide; according to the method, a pretreatment process combining microwave-assisted EDTA decalcification, ultrasonic-assisted ethanol degreasing and enzymolysis is adopted, and an immobilized enzyme membrane reactor and a multi-stage membrane separation technology are matched, so that efficient extraction and purification of the fish collagen peptide and resource utilization of byproducts are realized.

The invention relates to the field of electrochemical biosensors, in particular to a crosslinking process method of an enzyme membrane in a biosensor and a preparation method of an enzyme electrode. A cross-linking process method of an enzyme membrane in a biosensor comprises the following steps: spraying a cross-linking solution to the surface of the enzyme membrane, so that the fogdrop-shaped cross-linking solution permeates from the surface of the enzyme membrane to the inside of the enzyme membrane, and realizing different cross-linking densities of the inside and the outside of the enzyme membrane under the support of a polymer during incubation to obtain an enzyme electrode. According to the cross-linking process method of the enzyme membrane, the cross-linking reagent is atomized and evenly arranged on the enzyme membrane in a spraying mode, the prepared enzyme electrode is high in sensitivity and good in stability, meanwhile, operation is easy, batch production is easy, and the production cost is low.

The invention relates to a preparation method of an enzyme membrane sensor for detecting glutamine, and aims to provide a biosensor which can be matched with different redox electrodes for use, has general applicability and good performance and is suitable for online monitoring of glutamine. The enzyme membrane takes a polycarbonate filter membrane as a basement membrane, and is prepared by the following steps: fixing a nuclear track filter membrane on an O-shaped rubber ring, sequentially dropwise adding an enzyme solution on the filter membrane, and finally covering a layer of nuclear track filter membrane. The glutaminase membrane disclosed by the invention has relatively short response time and relatively stable operability, and meanwhile, the preparation process of the glutaminase membrane is simple and relatively low in cost.

This invention discloses an oligomeric wheat peptide and its preparation method, belonging to the field of plant peptide technology. The method uses wheat bran as raw material, which undergoes extrusion puffing and ultrafine grinding pretreatment, followed by alkaline extraction combined with amylase hydrolysis to obtain a wheat bran protein extract. Subsequently, it undergoes low-temperature controlled denaturation pretreatment, followed by freeze-thaw cycles to partially unfold the protein. Cysteine ​​is added as a conformation-locking agent to form reversible mixed disulfide bonds with exposed free thiol groups to stabilize the metastable conformation. Then, site-specific enzymatic hydrolysis is performed in an ultrasound-assisted enzyme membrane reactor, with ultrafiltration separating the oligopeptides in real time. Finally, electrodialysis desalination, nanofiltration concentration, and spray drying yield high-purity wheat bran oligomeric wheat peptides with gastric mucosal protective activity. This invention achieves high-value utilization of wheat bran components, with a green and efficient process, and produces peptides with high uniformity and strong activity, significantly improving the quality and market competitiveness of wheat peptide products.

The utility model relates to a biochemical sample treatment and analysis device, which comprises a box body, and a display module, a sample treatment module, a detection analysis module and a control system which are arranged in the box body, the sample treatment module comprises a sample box, a stock solution pool, a dilution pool and a pipetting platform positioned at the upper part of the box body, and a temperature control module is arranged below the sample box; the pipetting platform comprises a three-dimensional motion mechanism, a pipettor and a pipetting pump, the detection and analysis module comprises one or more of a photoelectric detection assembly, an ion detection assembly and an enzyme membrane detection assembly, and the control system is in communication connection with the sample processing module, the detection and analysis module and the display module.

The invention discloses a preparation method of high-stability potassium aspartate based on enzyme membrane reaction. The preparation method comprises the following steps: firstly, carrying out fermentation culture and immobilization treatment on enzyme-producing thalli; then, the immobilized microspheres are put into a conversion solution for low-temperature enzymatic reaction, a reaction system intercepts a target product in real time through membrane separation, and finally, stability enhancement and drying forming of the product are achieved under mild conditions through biological active molecule wrapping treatment. According to the process, the defects of high energy consumption, thermosensitive degradation and stability of a traditional method are overcome, and a closed-loop manufacturing system from biotransformation and resource recovery to product stability is formed.

A method for making an enzyme membrane for a working electrode of a continuous biological monitor includes making an aqueous silicone dispersion and making an acrylic polyol emulsion. The silicone dispersion and the acrylic polyol emulsion are mixed to make a base emulsion. An enzyme is added to the base emulsion to create an enzyme / base emulsion dispersion. The enzyme is selected according to a biological function to be monitored. The enzyme / base emulsion dispersion is applied to the working electrode. The applied enzyme / base emulsion dispersion is cured.

The utility model provides an enzymolysis-membrane filtration circulating device, particularly relates to the technical field of biological catalytic separation, and aims to solve the technical problem that the process of cleaning accumulated membrane pollution by regularly opening a reaction tank in the use process of an immobilized enzyme membrane reactor is very inconvenient. The device comprises a controller as well as a substrate solution tank, an immobilized enzyme reaction mechanism, a membrane separation mechanism and a product collection tank which are sequentially connected through pipelines, a pump body is arranged between the substrate solution tank and the immobilized enzyme reaction mechanism, and the front end of the membrane separation mechanism is connected with a waste liquid collection tank through a pipeline; a three-way reversing valve a is arranged between the membrane separation mechanism and the product collection tank, and one end of the three-way reversing valve a is connected with a backwashing mechanism. According to the device, through the arrangement of the backwashing mechanism and the three-way reversing valve, switching between a production mode and a membrane cleaning mode can be realized, and membrane pollution can be cleaned without opening the reaction tank through the backwashing mechanism in the membrane cleaning mode.

To measure diacetyl by simple biosensing.SOLUTION: Provided is a method for measuring diacetyl, including: in a state of infiltrating an enzyme membrane having diol dehydrogenase immobilized on a support membrane with an acidic solution containing reduced nicotinamide dinucleotide (NADH) as a coenzyme, and bringing the membrane into contact with a specimen fluid assumed to contain diacetyl as a substrate, irradiating the membrane with excitation light that excites NADH; detecting fluorescence emitted from NADH that is reduced by the enzyme reaction; and analyzing the concentration of diacetyl in the specimen fluid on the basis of the degree of decrease in the intensity of the detected fluorescence.SELECTED DRAWING: Figure 11

The invention discloses a method for preparing flaxseed oligosaccharide based on an enzyme membrane reactor, and belongs to the technical field of grain deep processing. According to the method for preparing the flaxseed oligosaccharide based on the enzyme membrane reactor technology, disclosed by the invention, the technical principle of the method is as follows: in the process of enzymolysis of flaxseed polysaccharide, oligosaccharide with a specific polymerization degree can be timely separated from a hydrolysate through a membrane with a specific molecular interception amount, that is, separation is carried out while enzymolysis is carried out so as to prevent excessive enzymolysis; therefore, the flaxseed oligosaccharide with high biological activity and uniform molecular weight distribution can be obtained. The method is simple and efficient, the production efficiency of obtaining the flaxseed oligosaccharides through enzymolysis is improved, and the obtained flaxseed oligosaccharides have high biological activity and uniform molecular weight distribution, have the advantages of high flux, low energy consumption, low processing cost, easiness in amplification and the like, and have great potential in the process of preparing the oligosaccharides on an industrial scale.

The invention belongs to the technical field of natural substance separation and extraction, and particularly relates to a haematococcus multiproduct gradient separation method based on deep eutectic solvent sequential extraction and enzyme membrane reactor coupling, which comprises the following steps: (1) cell wall weakening pretreatment; (2) extracting water-soluble components; (3) extracting pigment components; (4) an astaxanthin extraction step; and (5) enzymatic conversion. Through the synergistic effect of sequential extraction of the eutectic solvent and the enzyme membrane reactor, efficient, green and continuous gradient separation of water-soluble components, pigments, astaxanthin and xylooligosaccharide in haematococcus is realized, and the product purity and the resource utilization rate are improved.

The invention discloses a ginseng peptide liquid composition with a sleep improving function and a preparation method of the ginseng peptide liquid composition, and belongs to the technical field of health-care food. The composition is prepared from the following components in parts by weight: 15 to 20 parts of water boiling extracting solution of Chinese date, wheat and liquorice root, 0.01 to 0.05 part of ginseng nano peptide, 0.01 to 0.05 part of poria cocos peptide, 0.01 to 0.05 part of walnut peptide, 0.35 to 0.5 part of spina date seed extract and 0.5 to 1 part of passion flower nano powder. The preparation method integrates advanced technologies of dynamic countercurrent extraction, membrane separation, enzyme membrane bioreactor, nanocrystallization, non-thermal sterilization and the like, and specifically comprises the following steps: dynamic countercurrent extraction of a basic solution, microfiltration-ultrafiltration-nanofiltration membrane method combined refining, synergistic enzymolysis of an enzyme membrane reactor, nanocrystallization and compounding of key components, purification, purification and purification. And finally, sterilizing by ultrahigh pressure or ultraviolet-pulsed light, and carrying out sterile filling. The product is high in bioavailability, good in taste, good in stability, capable of remarkably shortening the sleep latency period and prolonging the sleep time, high in safety and free of traditional chemical drug dependence side effects, and an ideal medicine and food homology solution is provided for solving sleep disorders.

This invention relates to the field of bio-enzyme catalysis equipment technology, specifically a continuous flow enzyme catalysis reactor coupling online separation and enzyme membrane recovery. The reactor includes a reactor shell, the internal space of which is sequentially divided into a mixing zone, an enzyme membrane coupling zone, and a discharge zone along the material flow direction. A mixing component is disposed within the mixing zone; a separation component is disposed within the enzyme membrane coupling zone. The separation component includes multiple membrane filaments with enzyme catalysts fixed on their outer surfaces. Each membrane filament forms an independent product permeation channel, which is connected to the discharge zone. By placing a spiral stirring blade inside the membrane bundle formed by the membrane filaments, a gradient turbulence that gradually decreases from the inside out can be formed within the enzyme membrane coupling zone. This flow field can efficiently flush the enzyme-loaded layer on the outer surface of the membrane filaments, preventing substrate residues and enzyme aggregates from depositing and clogging micropores, maintaining high flux and high activity. It also avoids strong shear forces directly acting on the enzyme layer on the membrane surface, effectively protecting the stability of the enzyme.

The invention relates to the technical field of enzyme membrane reactors, and discloses a natural product enzyme membrane reactor, a stirring mechanism comprises: a rotating block connected to the top end of a movable shaft, the rotating block being elliptical; the movable rod is connected to the outer side of the rotating block; the stirring rod is connected to the inner side of the movable rod, and the stirring rod is cylindrical; the stirring block is arranged on the outer surface of the stirring rod. A movable shaft is driven by a working mounting motor of a mounting motor to contract, a connecting shaft pushes a movable plate to the outer side, and then the movable plate moves on a motor shaft to perform transverse position adjustment, so that the position of a stirring rod is adjusted, and the stirring rod is adjusted to a proper position; meanwhile, a driving unit in a rotating block drives a movable rod to rotate, the movable rod drives a stirring rod to rotate, and a stirring block arranged on the outer surface of the stirring rod stirs the material enzyme.

The invention discloses an immobilized enzyme membrane reaction system and application thereof in preparation of flaxseed oligosaccharides, and an immobilized enzyme membrane assembly comprises a polyethersulfone ultrafiltration membrane and polygalacturonase which are connected through a covalent bond; an integrated system with high catalytic efficiency, excellent operation stability and good membrane function retentivity is constructed through collaborative design and fine regulation and control of a carrier interface, immobilized chemistry and reaction-separation coupling conditions, so that the existing limitation is broken through, and the method is suitable for industrial production. And a feasible technical scheme is provided for green, efficient and continuous production of flaxseed oligosaccharide and other products.