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Ampere type biosensor electrode and method for making same

A biosensor and electrode technology, applied in instruments, scientific instruments, and material analysis through electromagnetic means, can solve problems such as theoretical immaturity, achieve increased anti-interference ability, high specific surface area, and widened linear response range Effect

Inactive Publication Date: 2008-10-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The third-generation sensor has completely eliminated external interference, but due to the immaturity of the theory, it is still under research and exploration.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) The acrylonitrile copolymer containing acrylic acid (viscosity average molecular weight 100,000, comonomer mole percentage is 5%) and ferrocene are co-dissolved in dimethylformamide, wherein the mass fraction of acrylonitrile copolymer is 2% , the mass of ferrocene accounts for 5% of the acrylonitrile copolymer. The platinum electrode was used as the receiving plate, and the fiber film was collected when the voltage was 10 kV, the extrusion rate was 0.5 ml / hour, and the receiving distance was 10 cm. Electrodes for iron composite nanofibrous membranes.

[0027] 2) Immerse the electrode surface deposited with acrylonitrile copolymer and ferrocene composite nanofiber film into 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinyl In the buffer solution of imine, the concentration of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride is 5 mg / ml, and the concentration of N-hydroxysuccinimide is 3 mg / ml, shake and activate at 25°C for...

Embodiment 2

[0029] 1) The acrylonitrile copolymer containing methacrylic acid (viscosity-average molecular weight 30,000, comonomer mole percentage is 20%) and porphyrin are co-dissolved in dimethylformamide, wherein the mass fraction of acrylonitrile copolymer is 15% %, the mass of the electronic mediator accounts for 80% of the acrylonitrile copolymer. The gold electrode was used as the receiving plate, and the fiber film was collected when the voltage was 20 kV, the extrusion rate was 3.0 ml / hour, and the receiving distance was 20 cm. The spinning time lasted 10 hours, and the deposited acrylonitrile copolymer and electronic media were obtained. Electrodes of bulk composite nanofibrous membranes.

[0030] 2) Immerse the electrode surface deposited with acrylonitrile copolymer and porphyrin composite nanofiber film into 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide amine buffer solution, wherein the concentration of 1-(3-dimethylaminopropyl)-3-eth...

Embodiment 3

[0032] 1) the acrylonitrile copolymer containing itaconic acid (30,000 viscosity-average molecular weight, 20% comonomer mole percent) and iron porphyrin are co-dissolved in dimethylformamide, wherein the acrylonitrile copolymer mass fraction is 15%, the mass of iron porphyrin accounts for 80% of the acrylonitrile copolymer. The glassy carbon electrode was used as the receiving plate, and the fiber film was collected when the voltage was 20 kV, the extrusion rate was 3.0 ml / h, and the receiving distance was 20 cm. The spinning time lasted 10 hours, and the deposited acrylonitrile copolymer and iron Electrodes for porphyrin composite nanofibrous membranes.

[0033]2) Immerse the electrode surface deposited with acrylonitrile copolymer and iron porphyrin composite nanofiber film into 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinyl In the buffer solution of imine, wherein the concentration of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydroch...

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Abstract

The invention discloses an ampere type biosensor electrode and a method for making the same. Dimethylformamide is taken as solvent, acrylonitrile copolymer and an electric mediator are taken as raw materials, an electrochemical electrode is taken as an accepting electrode, a composite fiber membrane of the acrylonitrile copolymer and the electric mediator is prepared on the surface of the electrode through electrostatic spinning, then a covalent method is used to fix xidoreductase on the surface of fibers to form an activated layer of the fibers on the surface of the electrode. The activated layer of the fibers has large specific surface area, high porosity rate and a through-hole structure; at the same time, the electric mediator is provided, so the electrode has higher response strength, linear detection range and stability. The mediator electrochemical enzyme electrode has the characteristics of simple preparation process, repeated use, large surface area of an effective enzyme membrane, strong anti-jamming ability and so on, and is suitable for batch production.

Description

technical field [0001] The invention relates to an ampere-type biosensor electrode and a preparation method thereof, in particular to a method for preparing an activation layer of a composite electron mediator by electrospinning. Background technique [0002] In electrochemical biosensors, the biosensitive membrane is a key part, which determines the quality and function of the sensor. Electrochemical sensors based on redox proteins and enzymes (hereinafter collectively referred to as enzymes) have been the mainstream of biosensor research. According to the electron transfer mechanism between enzymes and electrodes, electrochemical enzyme sensors can be roughly divided into three generations. Taking the glucose oxidase electrode as an example, the first generation of electrochemical sensors uses dissolved oxygen as the electronic channel between the enzyme and the electrode; the second generation uses artificially synthesized electronic mediators instead of dissolved oxygen...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 徐志康王振刚黄小军
Owner ZHEJIANG UNIV
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